Current Research in Biotechnology最新文献_第10页

Targeting the UCP1-dependent thermogenesis pathway with CRISPR/Cas9: a new approach to obesity management 利用CRISPR/Cas9靶向ucp1依赖性产热通路：肥胖管理的新途径

IF 3.6

Current Research in Biotechnology Pub Date : 2025-01-01 Epub Date: 2025-05-03 DOI: 10.1016/j.crbiot.2025.100295

Esmail Karami , Fatemeh Rostamkhani , Maasoume Abdollahi , Mohamadreza Ahmadifard

{"title":"Targeting the UCP1-dependent thermogenesis pathway with CRISPR/Cas9: a new approach to obesity management","authors":"Esmail Karami , Fatemeh Rostamkhani , Maasoume Abdollahi , Mohamadreza Ahmadifard","doi":"10.1016/j.crbiot.2025.100295","DOIUrl":"10.1016/j.crbiot.2025.100295","url":null,"abstract":"<div><div>Obesity is a complex, multifactorial disease characterized by excessive body fat accumulation, which negatively impacts health. Its increasing prevalence has led to a global epidemic, emphasizing the urgent need for innovative and effective treatment strategies. This study aims to explore the potential of CRISPR/Cas9-mediated gene editing to enhance UCP1-dependent thermogenesis, offering a novel approach to obesity management. Uncoupling protein 1 (UCP1), primarily located in the inner mitochondrial membrane of brown adipose tissue (BAT), plays a crucial role in thermogenesis and energy expenditure. By converting stored energy into heat, UCP1 activation enhances calorie burning, helping to regulate body temperature and mitigate obesity-related health risks. Recent advancements in genome editing technologies, particularly CRISPR/Cas9, provide a precise method to modify genes involved in UCP1 expression and activity. This approach holds significant promise for sustainable obesity management by enhancing metabolic efficiency and energy expenditure. This study examines the feasibility of using CRISPR/Cas9 to target the UCP1-dependent thermogenesis pathway for obesity treatment. It explores the mechanisms of CRISPR/Cas9, the role of UCP1 in energy regulation, and potential strategies to enhance thermogenic activity. Our findings highlight the promise of CRISPR-based interventions in metabolic regulation. However, further research is necessary to optimize safety, efficacy, and regulatory considerations before translating these findings into clinical applications.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100295"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143916719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Marine-derived secondary metabolites in oncology: A comprehensive review 肿瘤中的海洋次生代谢物：综合综述

IF 3.6

Current Research in Biotechnology Pub Date : 2025-01-01 Epub Date: 2025-05-22 DOI: 10.1016/j.crbiot.2025.100300

Samah S. Abuzahrah , Serag Eldin I. Elbehairi , Tahani Bakhsh , Ahmed Atwa , Nouf Juaid , Reham Hassan Mekky

{"title":"Marine-derived secondary metabolites in oncology: A comprehensive review","authors":"Samah S. Abuzahrah , Serag Eldin I. Elbehairi , Tahani Bakhsh , Ahmed Atwa , Nouf Juaid , Reham Hassan Mekky","doi":"10.1016/j.crbiot.2025.100300","DOIUrl":"10.1016/j.crbiot.2025.100300","url":null,"abstract":"<div><div>Marine-derived secondary metabolites are emerging as promising anticancer agents due to their structural diversity and potent bioactivity. Within the scope of this all-encompassing study, the most recent advancements in the identification, characterization, and therapeutic applications of marine-derived compounds in oncology are studied. In this review, we discuss various types of bioactive metabolites that are significant, such as macrolactins, polyketides, terpenes, and peptides that are derived from diverse marine organisms <em>viz</em>., bacteria, fungi, actinomycetes, microalgae, macroalgae, mangroves, sponges, coral reefs, mollusks, and echinodermata Unique compounds from marine organisms exhibit diverse mechanisms that selectively target cancer cells. This minimizes harm to healthy tissues and reduces side effects.“These metabolites interfere with key cancer progression pathways such as immune modulation, apoptosis, angiogenesis, metastasis, and cell cycle regulation. Additionally, they enhance conventional treatments like chemotherapy and immune therapy by overcoming drug resistance, particularly multidrug resistance (MDR) and the persistence of cancer stem cells (CSCs), major contributors to therapy failure. CSCs, characterized by self-renewal and therapy resistance, play a central role in tumor recurrence and drug resistance. This review highlights the novelty of marine metabolites, providing a comprehensive inventory of their clinical and commercial applications while emphasizing their role in advancing green medicine through sustainable drug discovery practices. This review shows the promise of marine-derived secondary metabolites in building the future of cancer therapies by incorporating insights from current studies. It also inspires further exploration of the development of these metabolites as clinically practicable treatments.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"10 ","pages":"Article 100300"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144169563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Workflow for evaluating enzyme immobilization and performance for continuous flow manufacturing 评估酶固定和连续流程生产性能的工作流程

IF 4

Current Research in Biotechnology Pub Date : 2025-01-01 Epub Date: 2025-08-16 DOI: 10.1016/j.crbiot.2025.100327

Emily M. Luteran, Marc R. Aloisi, Wendi L. Akerley, Robert D. Gilbertson

{"title":"Workflow for evaluating enzyme immobilization and performance for continuous flow manufacturing","authors":"Emily M. Luteran, Marc R. Aloisi, Wendi L. Akerley, Robert D. Gilbertson","doi":"10.1016/j.crbiot.2025.100327","DOIUrl":"10.1016/j.crbiot.2025.100327","url":null,"abstract":"<div><div>Enzymes have shown promise in various industries due to their functional specificity, catalytic efficiency, and environmental sustainability. These biological catalysts can be a pivotal component of manufacturing pipelines like continuous flow chemistry. For this, there exists a need to robustly immobilize enzymes on solid supports and assess the effects of the solid supports on catalytic performance and stability. Here, we use an industrially relevant model enzyme, <em>C. ensiformis</em> (Jack bean) urease, to demonstrate immobilization and assess performance in the context of continuous flow manufacturing. Various immobilization strategies were screened focusing on immobilization efficiency, protocol simplicity, and urease biocatalyst kinetics. Based on this, CDI-agarose and NHS-agarose resins were identified as the best-performing immobilization strategies for urease. CDI-agarose-urease and NHS-agarose-urease were then scaled up and applied to a large-scale continuous flow reactor to evaluate product yields, operational stability, and long-term stability. These experiments identified differences in stability and performance depending on the immobilization method tested. This highlights the importance of screening immobilization methods and subsequent enzyme performance for each candidate biocatalyst used in manufacturing to promote optimal performance and stability. As such, this work provides a framework for evaluating enzyme biocatalyst immobilization approaches to improve performance and enable transition into industrial processes.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"10 ","pages":"Article 100327"},"PeriodicalIF":4.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144866518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancing recombinant protein production by bacteria: strategies and challenges in heterologous systems 推进重组蛋白的细菌生产：策略和挑战在异源系统

IF 4

Current Research in Biotechnology Pub Date : 2025-01-01 Epub Date: 2025-10-17 DOI: 10.1016/j.crbiot.2025.100342

Devtulya Chander , Diksha Koul , Arushe Tickoo , Asha Chaubey

{"title":"Advancing recombinant protein production by bacteria: strategies and challenges in heterologous systems","authors":"Devtulya Chander , Diksha Koul , Arushe Tickoo , Asha Chaubey","doi":"10.1016/j.crbiot.2025.100342","DOIUrl":"10.1016/j.crbiot.2025.100342","url":null,"abstract":"<div><div>Recombinant protein production in bacteria is pivotal in industrial biotechnology, which enables scalable, cost-effective, and efficient synthesis of therapeutic proteins and enzymes. Present review explores key bacterial hosts including <em>Escherichia coli</em>, <em>Bacillus subtilis</em>, <em>Bacillus licheniformis</em>, and <em>Brevibacillus choshinensis</em> and more with an emphasis on extracellular heterologous protein production. Herein,we focus on secretion pathways such as Sec, Twin-arginine translocation (Tat) pathway, and ATP Binding Cassette (ABC) transporter systems, which govern protein export across membranes. The molecular strategies ranging from promoter optimization and codon adaptation to signal peptide engineering, strain modification, as well as protein fusion techniques have been highlighted that lead to improved secretion efficiency and product yields. Comparative insights into Gram-positive and Gram-negative systems, along with case studies, as well as importance of tailored approaches for different hosts and proteins have been reviewed. The review further discusses the growing relevance of CRISPR/Cas9n-based genetic interventions and the role of microbial physiology in facilitating optimized protein folding and transport. Overall, the integration of synthetic biology, secretion engineering, and host optimization offers promising avenues for advancing the extracellular production of high-value recombinant proteins.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"10 ","pages":"Article 100342"},"PeriodicalIF":4.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145415383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the innovation landscape of ginger: Insights from patent documentation analysis 探索生姜的创新前景：来自专利文件分析的见解

IF 3.6

Current Research in Biotechnology Pub Date : 2025-01-01 Epub Date: 2025-05-11 DOI: 10.1016/j.crbiot.2025.100299

Maima Matin , Bhupinder Kapoor , Monica Gulati , Michel-Edwar Mickael , Farhan Bin Matin , Agnieszka Kamińska , Joanna Bensz , Dariusz Sołdacki , Agnieszka Wierzbicka , Monika Marcinkowska-Lesiak , Ahmed Fatimi , Hong-Yan Liu , Ren-You Gan , Olga Adamska , Artur Stolarczyk , Jarosław Olav Horbańczuk , Artur Jóźwik , Atanas G. Atanasov

{"title":"Exploring the innovation landscape of ginger: Insights from patent documentation analysis","authors":"Maima Matin , Bhupinder Kapoor , Monica Gulati , Michel-Edwar Mickael , Farhan Bin Matin , Agnieszka Kamińska , Joanna Bensz , Dariusz Sołdacki , Agnieszka Wierzbicka , Monika Marcinkowska-Lesiak , Ahmed Fatimi , Hong-Yan Liu , Ren-You Gan , Olga Adamska , Artur Stolarczyk , Jarosław Olav Horbańczuk , Artur Jóźwik , Atanas G. Atanasov","doi":"10.1016/j.crbiot.2025.100299","DOIUrl":"10.1016/j.crbiot.2025.100299","url":null,"abstract":"<div><div><em>Zingiber officinale</em>, commonly known as ginger, holds a prominent place in both culinary and medicinal practices worldwide. This study is the first to present a comprehensive analysis of ginger-related patents, aiming to elucidate patterns and trends in innovation surrounding this versatile plant. The analysis, conducted using The Lens database, encompasses 7193 patent documents retrieved on April 26, 2025. Annual patent counts illustrate a significant increase in ginger-related patents over the years, with a notable rise observed since 2010. Analysis of the Cooperative Patent Classification system provides a structured framework for organizing these patents, revealing predominant covered categories such as food compositions, plant extracts, and tea substitutes. Additionally, the top-cited patents underscore the diverse applications of ginger, ranging from therapeutic formulations to food and beverage additives. Furthermore, examination of frequently mentioned diseases in ginger patents identifies respiratory ailments, digestive disorders, and skin conditions as key therapeutic areas. These findings show the multifaceted nature of ginger’s pharmacological properties and its potential in addressing various health challenges. Overall, by focusing on ginger as a key food and medicine plant, this study highlights the significance of patent analysis in understanding the innovation landscape of medicinal plants and nutraceuticals, and is specifically opening the ways for future research and development in ginger utilization for global health promotion and sustainable development.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100299"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144084190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Edible domestic animal blood products for biomedical and disease treatment applications 用于生物医学和疾病治疗的食用家畜血液制品

IF 3.6

Current Research in Biotechnology Pub Date : 2025-01-01 Epub Date: 2025-06-24 DOI: 10.1016/j.crbiot.2025.100308

Zhi Cao , Imran Mahmood Khan , Xiangrui Niu , Yang Hu , Zhuang Duan , Qiang Fang , Zhouping Wang , Junsong Yang

{"title":"Edible domestic animal blood products for biomedical and disease treatment applications","authors":"Zhi Cao , Imran Mahmood Khan , Xiangrui Niu , Yang Hu , Zhuang Duan , Qiang Fang , Zhouping Wang , Junsong Yang","doi":"10.1016/j.crbiot.2025.100308","DOIUrl":"10.1016/j.crbiot.2025.100308","url":null,"abstract":"<div><div>Edible blood products from domestic livestock are high-value derivatives produced during slaughter, with significant potential in biomedicine and disease treatment. Livestock blood is rich in proteins, peptides, iron, vitamins and other bioactive substances. Among them, functional proteins and peptides have been widely studied for their prominent roles in drug delivery, immune regulation, anti-infection and antioxidation. They significantly impact cancer treatment and immune regulation, providing valuable resources for innovation in the food and pharmaceutical industries. The development and utilization of livestock blood products offer innovative solutions for disease treatment and promote the sustainable development of the food and pharmaceutical industries by reducing the environmental burden of blood waste. However, challenges such as the complexity of blood composition, the variability of bioactive compound effectiveness, and the need for advanced purification techniques limit their broader applications. Additionally, regulatory and safety concerns must be addressed for therapeutic suitability. This review highlights the recent advancements in the biomedical applications of livestock blood products in biomedicine, addressing these challenges and emphasizing their potential to reduce environmental waste and promote sustainable development. This review consolidates current knowledge to provide a foundation for future research, fostering innovation in disease intervention and therapy while addressing utilization barriers.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"10 ","pages":"Article 100308"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144492004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenetically distinct fungal expansins show different binding preferences towards cellulosic materials and enhance cellulase activity 系统发育上不同的真菌扩张蛋白对纤维素材料表现出不同的结合偏好，并增强纤维素酶的活性

IF 3.6

Current Research in Biotechnology Pub Date : 2025-01-01 Epub Date: 2025-05-07 DOI: 10.1016/j.crbiot.2025.100296

Taru Koitto , Deepika Dahiya , Martina Huusela , Merja Penttilä , Emma Master

{"title":"Phylogenetically distinct fungal expansins show different binding preferences towards cellulosic materials and enhance cellulase activity","authors":"Taru Koitto , Deepika Dahiya , Martina Huusela , Merja Penttilä , Emma Master","doi":"10.1016/j.crbiot.2025.100296","DOIUrl":"10.1016/j.crbiot.2025.100296","url":null,"abstract":"<div><div>Expansins and expansin-like proteins are found in plants and microbes, and can disrupt the cellulosic microfibril network of plant cell walls. While plant expansins play a role in cell wall formation, microbial expansin-like proteins reportedly enhance the activity of lignocellulolytic enzymes. Herein, two novel fungal expansin-like proteins, <em>Ama</em>EXLX1 from <em>Allomyces macrogynus</em> and <em>Apu</em>EXLX1 from <em>Aureobasidium pullulans</em>, were recombinantly produced in <em>Pichia pastoris</em>. While both <em>Ama</em>EXLX1 and <em>Apu</em>EXLX1 retain typical expansin structure, they share low sequence identity (22.5 %) and different predicted pI values (5.8 and 8.8, respectively), which was expected to impact their action on cellulosic substrates. Accordingly, adsorption of the proteins on cellulose nanofibrils (CNF) and the impact of the expansin-like proteins on the hydrolysis of CNF by an endoglucanase (Cel7B) was investigated using quartz crystal microbalance with dissipation (QCM-D). <em>Ama</em>EXLX1 showed higher affinity towards cellulose compared to <em>Apu</em>EXLX1, which was correlated to missing key aromatic residues in the polysaccharide binding surface of <em>Apu</em>EXLX1. The pretreatment of a CNF film with <em>Ama</em>EXLX1 and <em>Apu</em>EXLX1 increased the initial rate of Cel7B activity. This study underscores similarities between the impacts that bacterial and fungal expansin-like proteins can have on the enzymatic deconstruction of cellulose, and sequence properties that could impact expansin performance.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100296"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Termitomyces mushroom extract-mediated synthesis of silver nanoparticles and its in-vitro activity against drug-resistant Candida species 白蚁菌蘑菇提取物介导的纳米银合成及其对耐药念珠菌的体外活性研究

IF 3.6

Current Research in Biotechnology Pub Date : 2025-01-01 Epub Date: 2025-02-14 DOI: 10.1016/j.crbiot.2025.100279

Naheem Adekilekun Tijani , Joseph Hokello , Emmanuel Eilu , Saheed Adekunle Akinola , Abdullateef Opeyemi Afolabi , Ibrahim Ntulume , Ismail Abiola Adebayo

{"title":"Termitomyces mushroom extract-mediated synthesis of silver nanoparticles and its in-vitro activity against drug-resistant Candida species","authors":"Naheem Adekilekun Tijani , Joseph Hokello , Emmanuel Eilu , Saheed Adekunle Akinola , Abdullateef Opeyemi Afolabi , Ibrahim Ntulume , Ismail Abiola Adebayo","doi":"10.1016/j.crbiot.2025.100279","DOIUrl":"10.1016/j.crbiot.2025.100279","url":null,"abstract":"<div><div>Green nanotechnology has continued to gain popularity as a novel and alternative strategy to overcome the menace caused by drug-resistant pathogens. For the first time, this study explores an efficient, eco-friendly, and economical approach for the mycogenic synthesis of silver nanoparticles (AgNPs) by utilizing the aqueous extract of wild <em>Termitomyces</em> species of edible mushroom. The mushroom-assisted AgNPs synthesis was validated with visual colour observation and characterized with UV–Vis spectrophotometer, SEM, EDX, XRD, FTIR and DLS. The potential anticandidal efficacy of the synthesized AgNPs was investigated against six clinical isolates of resistant pathogenic <em>Candida</em> species. A typical Ag surface plasmon resonance (SPR) had absorbance maxima wavelength within 371–404 nm range, with a spherical shape particulate structure in the size range of 28 to 45 nm according to UV–Vis and SEM analyses respectively. Remarkable antifungal activity was recorded against a good number of the <em>Candida</em> isolates with MICs values in the range of 0.0122–0.0976 mg/mL. We conclude that wild <em>Termitomyces</em> mushroom is a suitable biomaterial for AgNPs synthesis and an effective antifungal agent which could be adopted as a novel therapeutic agent for efficient management of drug-resistant <em>Candida</em> pathogens.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100279"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The public discourse on genetics in autism communities: a content analysis of Reddit posts 自闭症群体中关于遗传学的公开讨论：对Reddit帖子的内容分析

IF 4

Current Research in Biotechnology Pub Date : 2025-01-01 Epub Date: 2025-10-22 DOI: 10.1016/j.crbiot.2025.100346

Kamil R. Hiralal , Anneke Louwerse , Matea Skojo , Manon H.J. Hillegers , Hugo G. Schnack , Sabine E. Mous , Gwendolyn C. Dieleman

{"title":"The public discourse on genetics in autism communities: a content analysis of Reddit posts","authors":"Kamil R. Hiralal , Anneke Louwerse , Matea Skojo , Manon H.J. Hillegers , Hugo G. Schnack , Sabine E. Mous , Gwendolyn C. Dieleman","doi":"10.1016/j.crbiot.2025.100346","DOIUrl":"10.1016/j.crbiot.2025.100346","url":null,"abstract":"<div><h3>Purpose</h3><div>There is ongoing debate in the autism community on the role of genetics. Most research examining the discourse on genetics in autism have focused primarily on caregivers. This study explored the public discourse on genetics within the broader autism community.</div></div><div><h3>Methods</h3><div>We collected 30,014 Reddit posts on genetics within autism communities, since the platform’s inception through August 1, 2024. Using topic modelling, we identified key topics and we conducted sentiment analysis to compare the emotional tone across these topics. Finally, we examined how user characteristics were related to the sentiment of a post.</div></div><div><h3>Results</h3><div>We identified seven topics related to genetics: <em>personal stories, place in society, parental support, familial inheritance, healthcare, research,</em> and <em>moderator messages.</em> Threads on healthcare and personal stories were relatively negative, whereas research and moderator messages threads were more positive. Comments on healthcare were relatively negative, while parental support and moderator messages comments were more positive. Users who generally post more positively also expressed more positive sentiment when discussing genetics in the context of autism.</div></div><div><h3>Conclusions</h3><div>The online discourse on genetics in online autism communities encompasses multiple topics. Researchers and clinicians should continuously engage with the autism community to address concerns regarding genetics in autism.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"10 ","pages":"Article 100346"},"PeriodicalIF":4.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145361513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of network pharmacology integrated with experimental approaches in natural products drug discovery 网络药理学与实验方法在天然产物药物发现中的作用

IF 4

Current Research in Biotechnology Pub Date : 2025-01-01 Epub Date: 2025-09-29 DOI: 10.1016/j.crbiot.2025.100338

Anoop Kumar, Devesh Tewari

引用次数: 0