Current Research in Biotechnology最新文献

{"title":"Genomic insights on gene clusters and pathways for the biodegradation of plastic compounds: Unravelling the metabolic versatility in a Dietzia kunjamensis IITR165","authors":"Saurabh Singh ,&nbsp;Srikrishna Subramanian ,&nbsp;Neha Gupta ,&nbsp;Abhay Bajaj ,&nbsp;Natesan Manickam","doi":"10.1016/j.crbiot.2024.100258","DOIUrl":"10.1016/j.crbiot.2024.100258","url":null,"abstract":"<div><div>The <em>Dietzia kunjamensis</em> IITR165 bacterium, capable of degrading dibutyl phthalate (DBP), terephthalate (TPA), and polyethylene terephthalate (PET), was studied to uncover its metabolic pathways. Whole-genome analysis revealed a circular chromosome of 3,477,711 bp and a plasmid of 58,850 bp with 70.6 % GC content. Among 3,311 functional genes, phthalate dioxygenase/decarboxylase (<em>pad</em>Aa1, <em>pad</em>Ab1, <em>pht</em>B, <em>pht</em>C), alkane monooxygenase (<em>alk</em>B), di-and mono-alkyl phthalate hydrolase, and extra-diol dioxygenase were identified. Gene clusters for terephthalate (<em>tph</em>A1A2A3 and <em>tph</em>B), benzoic acid (<em>ben</em>ABCD), and catechol (<em>cat</em>ABCD) were also found. Strain IITR165 metabolized of 1000 mg/L of TPA in 96 h with a half-life of 15.36 h⁻¹, producing phthalic acid (PA), benzoic acid (BA), and catechol as metabolites based on Q-TOF LC/MS-MS analysis. Scanning electron micrograph reveals the extensive biofilm and surface modification of PET sheet after bacterial treatment. A novel PET-hydrolase (PET165) protein, sharing 45.70 % amino acid homology with reported PETases, was discovered, with docking studies showing a conserved catalytic triad (Serine-128, Aspartate-261, and Histidine-287) interacting with the PET ligand. The presence of this novel PET hydrolase and the <em>tpa</em> gene cluster, along with genes involved in nylon, and polystyrene metabolism, indicates versatility of the bacterium useful in treatment of a mixed plastic contaminated ecological niches.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"8 ","pages":"Article 100258"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142421327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the potential of marine-derived diterpenes from the order Alcyonacea as promising anti-obesity agents","authors":"Mohamed A. Tammam ,&nbsp;Omnia Aly ,&nbsp;Florbela Pereira ,&nbsp;Aldoushy Mahdy ,&nbsp;Amr El-Demerdash","doi":"10.1016/j.crbiot.2024.100175","DOIUrl":"10.1016/j.crbiot.2024.100175","url":null,"abstract":"<div><p>Metabolic syndrome (MS) represents a global health challenge characterized by various metabolic disorders, including HOMA-IR (insulin resistance), obesity, dyslipidemia, and hypertension. In our pursuit of identifying natural alternatives for the development of effective and safe anti-obesity medications, we examined the potential of the methanolic extract of the Red Sea derived soft coral <em>Sarcophyton glaucum</em>, where serum levels of glucose, insulin, HOMA-IR, lipid profile, fetuin A and B, PTP1Β (Protein tyrosine phosphatase 1B), adropin and omentin were determined. Furthermore, the expression of the UCP1 (Uncoupling protein 1) and PPARGC1A (Peroxisome proliferator–activated receptor-g coactivator-1a) genes have been assessed, to evaluate the anti-obesity potential of <em>S. glaucum</em> organic extract. Our findings demonstrated a significant decrease in glucose, HOMA-IR, cholesterol, triglyceride, LDL-C, fetuin A and B, and PTP1Β levels, accompanied by a significant increase in insulin, HDL-C, adropin, omentin, UCP1, and PPARGC1A expression after treatment with the soft coral extract. These promising outcomes can be attributed to the remarkable ingredients present in the extract, which were further supported by histopathological findings. In addition, a virtual screening protocol including molecular docking (MDock) and Structure-Activity Relationships (SARs) of 27 marine diterpenes was also explored to identify potential PTP1Β inhibitors targeting simultaneously the catalytic site and allosteric site, as well as fetuin A modulators. Moreover, the six most promising predicted marine diterpenes (<strong>4</strong>, <strong>8</strong>, <strong>9</strong>, <strong>10</strong>, <strong>13</strong> and <strong>14</strong>) were investigated for their pharmacokinetic properties, druglike nature and medicinal chemistry friendliness using the SwissADME platform. Of these, four marine diterpenes (<strong>4</strong>, <strong>8</strong>, <strong>9</strong>, and <strong>10</strong>) were predicted to exhibit the appropriate drug-like properties.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100175"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000017/pdfft?md5=2846ee09908cfe478758346b5c71fdf9&pid=1-s2.0-S2590262824000017-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139454753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Melanoma efficacy of traditional multi-herbal extracts from mongolian ethnomedicine on B16F10 murine cells","authors":"Yerkegul Dauletkhan ,&nbsp;Tae Young Han ,&nbsp;Janbolat Ashim ,&nbsp;Shukherdorj Baasanmunkh ,&nbsp;Altantsetseg Khajidsuren ,&nbsp;Wookyung Yu ,&nbsp;Purevjargal Naidansuren ,&nbsp;Uteubayeva Gulzada ,&nbsp;Kang Duk Choi ,&nbsp;Baatartsogt Oyungerel","doi":"10.1016/j.crbiot.2024.100217","DOIUrl":"10.1016/j.crbiot.2024.100217","url":null,"abstract":"<div><p>Multi-herbal formulation is an attractive approach to developing novel therapeutic strategies to manage advanced forms of melanoma. This research aims to evaluate the anti-melanoma potential of Traditional Multi-Herbal (G4) Extracts sourced from Mongolian Ethnomedicine utilizing both cellular and xenograft models. In vitro and ex vivo experiments employing B16F10 melanoma cells were conducted to evaluate the anti-cancer effect of the G4 extract. Furthermore, in vivo experiments utilizing BALB/C nu/nu mice xenograft models were carried out to gauge the extract's effectiveness. A comprehensive analysis encompassing various assays, such as cell viability, migration and invasion assays, cellular phase analysis, and key indicators of apoptosis, was performed. These indicators included activation of the caspase-3 cascade, genomic DNA fragmentation, nuclear staining alterations, and levels of cell cycle and apoptotic regulatory markers analysis. Our Results showed that the G4 extract exhibited potent anti-cancer effects on B16F10 melanoma cells, notably inhibiting cell migration and vascular sprouting in a concentration-dependent manner, suggesting its potential to impede melanoma metastasis. This investigation underscores the promising anti-cancer potential of the G4 extract against melanoma cells through the modulation of apoptotic pathways and suppression of tumor xenograft growth. Ultimately, our findings suggest that the G4 extract holds promise as a candidate for the development of future melanoma chemotherapeutics.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100217"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000431/pdfft?md5=6be3d5482a58c8afeca187622f7aff5b&pid=1-s2.0-S2590262824000431-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140774086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery of novel plant biomass conversion associated fungal transcription factors using a network-based approach","authors":"Mao Peng ,&nbsp;Astrid Mueller ,&nbsp;Joanna E. Kowalczyk ,&nbsp;Roland S. Kun ,&nbsp;Ronald P. de Vries","doi":"10.1016/j.crbiot.2024.100230","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100230","url":null,"abstract":"<div><p>Fungal plant biomass conversion (FPBC) is an important component of the global carbon cycle and has been widely applied for the production of biofuels, enzymes and biochemicals. Identification of transcription factors (TFs) governing FPBC is crucial for genetic engineering of industrial fungi towards sustainable production of high-value bioproducts from renewable lignocellulose. Here, we developed a bioinformatics framework for the identification of FPBC related TFs based on reconstructed gene regulatory networks and enrichment analysis of manually curated<!--> <!-->FPBC gene sets. Applying this approach to model fungi <em>Aspergillus niger</em> and <em>Neurospora crassa,</em> we successfully identified both known TFs and promising candidates. The function of one identified TF, HapX, has been experimentally validated, and several candidates were supported by literature, transcriptome data and initial growth analysis. Our new approach will accelerate the identification of novel TFs involved in FPBC, and facilitate the further improvement of fungal cell factories.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"8 ","pages":"Article 100230"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S259026282400056X/pdfft?md5=c721f7d92590436a920a033573fda5c4&pid=1-s2.0-S259026282400056X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141438434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnolol alleviates inflammation and oxidative stress in COPD via MAPK and Nrf2 signaling pathways","authors":"Simei Liu ,&nbsp;Chonggang Huang ,&nbsp;Zhen Wu ,&nbsp;Xiaoli Mei ,&nbsp;Sheng Li ,&nbsp;Yajing Xing ,&nbsp;Yadong Zhong ,&nbsp;Benzhong Mi ,&nbsp;Yongde Wang ,&nbsp;Xin Tang","doi":"10.1016/j.crbiot.2024.100264","DOIUrl":"10.1016/j.crbiot.2024.100264","url":null,"abstract":"<div><div>Chronic obstructive pulmonary disease (COPD) is a chronic and progressive lung disease which is closely related to inflammation and oxidative-stress. Magnolol (MAG) is a polyphenolic compound that possesses extensive pharmacological actions, including anti-inflammatory and anti-oxidative activities. However, the therapeutic role of MAG in COPD remains unclear. This study aimed to explore the effect and underlying mechanism of MAG in COPD using lipopolysaccharide (LPS) and cigarette smoke (CS)-induced rats model, LPS-induced RAW264.7 cells, and CS extract (CSE)-induced epithelial BEAS-2B cells. Here, MAG significantly alleviated the symptoms and lung pathological changes of COPD rats by regulating the inflammatory response and oxidative stress. Moreover, it attenuated inflammation by suppressing the production of pro-inflammatory cytokines (NO, IL-6, IL-1β, and TNF-α) in LPS-induced RAW264.7 cells, and relieved CSE-induced oxidative stress by reducing ROS accumulation and increasing the levels of antioxidant enzymes (SOD, HO-1, and NQO1) in BEAS-2B cells. Mechanistically, MAG inhibited MAPK signaling pathway via down-regulating the phosphorylation level of p38, ERK1/2 and JNK, and activated Nrf2 signaling pathway via promoting Nrf2 nucleus translocation and subsequently up-regulating the protein expressions of HO-1, NQO1, and Keap1 <em>in vitro</em> and <em>in vivo</em>. Collectively, these findings demonstrated that MAG may be a promising candidate for COPD treatment.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"8 ","pages":"Article 100264"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142703866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumin prevents cadmium or H2O2-induced oxidative stress via Nrf2/ARE signaling and autophagy in myeloid cells","authors":"Maria Russo,&nbsp;Annamaria Di Giacomo ,&nbsp;Federica Fiore ,&nbsp;Carmela Spagnuolo,&nbsp;Virginia Carbone,&nbsp;Paola Minasi,&nbsp;Gian Luigi Russo","doi":"10.1016/j.crbiot.2024.100266","DOIUrl":"10.1016/j.crbiot.2024.100266","url":null,"abstract":"<div><div>The evidence linking high levels of environmental pollutants to chronic degenerative diseases is alarming, with heavy metals (HM) identified as a key factor. Research suggests that certain phytochemicals in the diet can reduce HM levels and mitigate their adverse health effects.Curcumin (Curc), a natural polyphenol, is particularly effective in protecting against Cadmium (Cd) toxicity. The present study demonstrates that preincubation with low doses of Curc (1 μM) in differentiated HL-60 and K-562 human myeloid cells can significantly protect against cytotoxicity induced by Cd and or hydrogen peroxide (H₂O₂). Curc reduced the increased levels of Reactive Oxygen Species (ROS) generated by Cd or H₂O₂ by inducing a protective form of autophagy, a relevant process for the cellular homeostasis. Curc activated mild oxidative stress that triggers the expression of Nrf2-dependent transcripts, such as Nuclear factor erythroid 2-related factor 2 (Nrf2)-dependent transcripts, such as human NADPH Quinone Oxidoreductase 1 (NQO1) and Heme Oxygenase (HO1). The potential chemopreventive effects of Curc against redox stress have been strengthened by the observation that free and unmetabolized Curc is detectable inside the cells after 5 min of treatment, and its presence parallels with increased levels of intracellular Glutathione (GSH). These findings suggest that supplementation with Curc in the form of nutraceuticals may represent a promising way to protect people living in highly polluted areas against the adverse effects of HM contaminants.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"8 ","pages":"Article 100266"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142703868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Postbiotics, a natural feed additive for growth performance, gut microbiota and quality of poultry products","authors":"Jakub Urban ,&nbsp;Karwan Yaseen Kareem ,&nbsp;Atanas G. Atanasov ,&nbsp;Arkadiusz Matuszewski ,&nbsp;Damian Bień ,&nbsp;Patrycja Ciborowska ,&nbsp;Anna Rygało-Galewska ,&nbsp;Monika Michalczuk","doi":"10.1016/j.crbiot.2024.100247","DOIUrl":"10.1016/j.crbiot.2024.100247","url":null,"abstract":"<div><p>Implementing global strategies for sustainable poultry production is imperative, considering the continuous growth of the human population and the subsequent rise in demand for poultry products, which must be fully safe for future consumers. Identifying and implementing safe, environmentally friendly, and cost-effective alternatives such as postbiotics have become imperative in sustainable poultry farming. This is primarily due to the increasing apprehensions surrounding antimicrobial resistance, the need to minimise or eliminate the use of antibiotic growth promoters, and the growing consumer preference for products that are free from chemicals and antibiotics. Postbiotics refer to bioactive compounds that are synthesised by lactic acid bacteria during the process of metabolising prebiotics. These compounds, also known as biogenics or metabolites, are soluble factors that offer notable health benefits. This concept is a recent development in the field of animal nutrition. Postbiotics possess modes of action and capabilities similar to probiotics, albeit lacking live cells, owing to the presence of probiotic secondary metabolites. These encompass several pharmacologically active compounds that positively influence the performance and quality of poultry products. This article examines the concept, impact and approaches associated with using an innovative solution such as postbiotics in the context of sustainable poultry production.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"8 ","pages":"Article 100247"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S259026282400073X/pdfft?md5=9078633b45cb341b0a8a85186f57cd48&pid=1-s2.0-S259026282400073X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142129132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"All-trans-retinoic acid and valproic acid: A combinatorial approach for the treatment of nephrotic syndrome","authors":"Rini Varghese,&nbsp;Anuradha Majumdar","doi":"10.1016/j.crbiot.2024.100181","DOIUrl":"10.1016/j.crbiot.2024.100181","url":null,"abstract":"<div><p>Massive proteinuria, oedema, hypoalbuminemia, and hyperlipidemia are the hallmarks of nephrotic syndrome (NS). Recently, epigenetic pathways in renal diseases have been identified. The present work hypothesizes using the combination of two epigenetic drugs all-trans retinoic acid (ATRA) and valproic acid (VPA) as a prospective treatment method to lessen NS-related glomerulosclerosis, fibrosis, and increased renal function parameters along with attenuating inflammation and improving overall mitochondrial health. To induce NS, doxorubicin (8.5 mg/kg, n = 6) was injected intravenously into female Sprague Dawley rats. After 28 days, ATRA and VPA were orally administered to the rats, alone and in combination at a dose of 5 mg/kg (n = 6) and 200 mg/kg (n = 6) in sesame oil and saline, respectively. Prednisolone (3 mg/kg in saline; n = 6) was used as standard. Following 21-day<!--> <!-->treatment period, the rats were sacrificed prior to which 24<!--> <!-->hrs urine samples were obtained. Blood samples were collected and kidneys were extracted for further analyses. Renal function parameters (proteinuria, BUN, albumin, creatinine), levels of tissue reengineering and fibrosis markers (TGF-β, MMP2 activity), cholesterol and triglyceride levels were significantly improved in the ATRA and VPA combination group as compared to positive control group. Histopathological analyses revealed a reduction in inflammation, glomerulosclerosis and fibrosis. The inflammatory markers, namely TNF-α, IL-1β, IL-6, NF-κB, determined by ELISA were downregulated. Mitochondrial biogenesis markers viz. PGC-1α, TFAM, NRF1, Nrf2, PPAR-γ, KEAP-1, analysed by RT-qPCR were upregulated thereby showing a significant improvement in the combination group as compared to positive control and standard group. The study overall contributes to a novel approach to treating NS and our findings will surely drive additional exploratory preclinical and clinical studies.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100181"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000078/pdfft?md5=2946a84ad7538fc9bb75fd3e0e7870f9&pid=1-s2.0-S2590262824000078-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139631574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungal pellets of Pleurotus ostreatus and Rhizopus stolonifer for biotechnological applications: Characterization and production optimization using Taguchi methodology","authors":"Thabata Montserrat Hernández-Cruz ,&nbsp;Adriana Jazmín Legorreta-Castañeda ,&nbsp;Karina García-Gutiérrez ,&nbsp;Marco Polo Carballo-Sánchez ,&nbsp;Guadalupe Guerra-Sánchez ,&nbsp;Dario Rafael Olicón-Hernández","doi":"10.1016/j.crbiot.2024.100226","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100226","url":null,"abstract":"<div><p>Filamentous fungi are known for their significant potential in biotechnology, thanks to their versatile enzyme systems with various applications. However, dealing with their growth patterns and structural configurations presents significant challenges. To tackle these issues, fungal pellets are emerging as potential solutions, providing compact biomass aggregates that offer distinct advantages for bioprocesses. This study delves into characterization and optimizing pellet formation for <em>Pleurotus ostreatus</em> and <em>Rhizopus stolonifer</em> using the Taguchi methodology, aiming to enhance their biotechnological applications. By systematically varying parameters such as agitation level (AL), glucose concentration (GC), and inoculum size (IS), we identified key factors influencing pellet formation. Results indicate that <em>P. ostreatus</em> forms pellets in rich media from mycelium, while <em>R. stolonifer</em> requires a minimal medium with pH modifications for pelletization via a coagulative mechanism. The optimization process reveals that agitation level is a crucial factor for maximizing pellet production in both models, while the other factors do not seem to influence the process significantly but impact the morphology and quantity of pellets. The results suggest that by optimizing parameters using the Taguchi method, it is possible to achieve acceptable pellet formation performance in both fungi. Understanding these factors is essential for improving the efficiency of biotechnological processes involving fungal biomass, providing valuable insights into enhancing fungal pellet production for various applications.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100226"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000522/pdfft?md5=ef90fc2feefac7698810534899bd7b76&pid=1-s2.0-S2590262824000522-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140918270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Weak electrostimulation to enhance planktonic and biofilm microbial interactions on complex carbon degradation for biogas recovery","authors":"Zhe Yu ,&nbsp;Wenzong Liu ,&nbsp;Xiqi Li ,&nbsp;Bin Liang ,&nbsp;Jiexu Ye ,&nbsp;Aijie Wang","doi":"10.1016/j.crbiot.2024.100214","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100214","url":null,"abstract":"<div><p>Electrode biofilm and planktonic microorganisms are two important parts in bioelectrochemical system, but the mechanism of planktonic microorganisms affect biofilms is not clear. Waste sludge lysate produced from sludge pyrolysis contains melanoidins as complex carbon source that are refractory. Anaerobic digestion companied with weak electrostimulation is adopted to treat the wastewater and explore the mechanism in electron transfer, biofilm activity and microbial community structure. In the presence of planktonic microorganisms, electrode biofilm still plays a major role that about 70% of organics was removed by biofilm, the energy recovery efficiency increased by 10.81%, and the main electron transfer mechanism of biofilm is unchanged, but extra electron transfer pathway (E_f = 0.404 V vs Ag/AgCl) is generated. More cytochrome is produced in biofilm and charge transfer resistance decreases to facilitate electron transfer. The planktonic microorganisms cause more electrochemically active microorganisms and methanogens enrich in the electrode biofilm to enhance the direct interspecies electron transfer.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100214"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000406/pdfft?md5=82570747994afc6292937e1c847329fe&pid=1-s2.0-S2590262824000406-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140650690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}