Current Research in Biotechnology最新文献

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Eco-engineered remediation: Microbial and rhizosphere-based strategies for heavy metal detoxification 生态工程修复:基于微生物和根际的重金属解毒策略
IF 3.6
Current Research in Biotechnology Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100297
Arun Karnwal , Gaurav Kumar , Alaa El Din Mahmoud , Joydeep Dutta , Rattandeep Singh , Abdel Rahman Mohammad Said Al-Tawaha , Tabarak Malik
{"title":"Eco-engineered remediation: Microbial and rhizosphere-based strategies for heavy metal detoxification","authors":"Arun Karnwal ,&nbsp;Gaurav Kumar ,&nbsp;Alaa El Din Mahmoud ,&nbsp;Joydeep Dutta ,&nbsp;Rattandeep Singh ,&nbsp;Abdel Rahman Mohammad Said Al-Tawaha ,&nbsp;Tabarak Malik","doi":"10.1016/j.crbiot.2025.100297","DOIUrl":"10.1016/j.crbiot.2025.100297","url":null,"abstract":"<div><div>Heavy metal (HM) contamination significantly threatens ecosystems and human health. This review explores eco-engineered bioremediation strategies, focusing on the pivotal role of rhizosphere-associated microorganisms in detoxifying heavy metals. Rhizobacteria deploy diverse mechanisms—including biosorption, bioaccumulation, biotransformation, and biomineralization—to immobilize or convert toxic metals, with their efficiency strongly influenced by environmental factors such as pH and metal speciation. Plant Growth-Promoting Rhizobacteria (PGPR) further enhance phytoremediation by mitigating metal-induced phytotoxicity and promoting plant resilience under stress. Various scalable approaches, including in-situ and ex-situ remediation techniques, biosorbents, microbial consortia, and genetically engineered microbes (GEMs), show promising potential but raise essential ecological and regulatory concerns. Key challenges such as scalability, environmental variability, and the possible formation of toxic intermediates must be carefully addressed. Advances in omics technologies and a deeper exploration of native microbial communities offer promising avenues to optimize bioremediation outcomes. Moreover, a detailed understanding of plant–microbe interactions and the role of secondary metabolite signalling in the rhizosphere is essential to improve remediation efficiency. Future strategies should prioritize the application of functional genomics, developing bioinoculants tailored to specific environmental conditions, and implementing robust ecological risk assessments for GEMs. This review underscores the need for a multidisciplinary approach- integrating microbial ecology, plant sciences, and environmental engineering- to drive the development of sustainable, effective HM remediation technologies worldwide.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100297"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Peptides and peptidomimetics in the development of hydrogels towards the treatment of diabetic wounds 水凝胶在糖尿病伤口治疗中的应用
IF 3.6
Current Research in Biotechnology Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100292
Ana Gomes, Paula Gomes
{"title":"Peptides and peptidomimetics in the development of hydrogels towards the treatment of diabetic wounds","authors":"Ana Gomes,&nbsp;Paula Gomes","doi":"10.1016/j.crbiot.2025.100292","DOIUrl":"10.1016/j.crbiot.2025.100292","url":null,"abstract":"<div><div>Diabetic foot ulcers are a major complication of diabetes and a particularly complex type of chronic wounds, as most diabetes-related physiological dysregulations impair healing and increase the risk of infection. Despite many health literacy initiatives have been deployed to promote both the prevention and the proper management of diabetic foot ulcers, these remain a serious healthcare problem whose incidence is rising. This demands not only for a continuous updating of guidelines for adequate interventions in healthcare facilities, but also for new topical treatments able to improve the health and well-being of the patients. In this later case, current options include topical oxygen therapy, negative pressure wound therapy, wound dressings of different types, and topical gels. Due to the tremendous potential of hydrogel-based dressings for diabetic wound treatment, several hydrogels from either natural or synthetic origin have been explored. The hydrogel polymeric matrices can be loaded with many diverse molecules (cargoes) eventually combining distinct therapeutic actions. In this context, peptides are gaining relevance, which is not limited to those used as bioactive cargoes, since self-assembling peptides able to form hydrogels are also under the spotlight. This review focuses on the studies reported over the last decade where peptide-loaded or peptide-derived hydrogels have been tested <em>in vivo</em> for topical treatment of diabetic wounds and further explores the potential of peptidomimetics to expand the portfolio of hydrogel-based treatments for diabetic foot ulcers.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100292"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143879009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Construction of targeting GPR77+CD10+ lipid nanoparticles and validation of targeting capability in vitro and in vivo 靶向GPR77+CD10+脂质纳米颗粒的构建及体外和体内靶向能力的验证
IF 3.6
Current Research in Biotechnology Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100291
Junyue Fang , Qiongchao Jiang , Xinyu Yang , Weifan Li , Li Lin , Meng Zhang , Phei Er Saw , Xiaoyun Xiao
{"title":"Construction of targeting GPR77+CD10+ lipid nanoparticles and validation of targeting capability in vitro and in vivo","authors":"Junyue Fang ,&nbsp;Qiongchao Jiang ,&nbsp;Xinyu Yang ,&nbsp;Weifan Li ,&nbsp;Li Lin ,&nbsp;Meng Zhang ,&nbsp;Phei Er Saw ,&nbsp;Xiaoyun Xiao","doi":"10.1016/j.crbiot.2025.100291","DOIUrl":"10.1016/j.crbiot.2025.100291","url":null,"abstract":"<div><div>Dual-receptor targeting strategies hold promise for improving nanocarrier specificity in complex tumor microenvironments. Herein, we engineered lipid nanoparticles (LNPs) functionalized with GPR77 and CD10 antibodies to exploit receptor co-expression as a mechanism for enhanced targeting. To rigorously validate this approach, we developed a GPR77<sup>+</sup>CD10<sup>+</sup> overexpressing CHO cell model, which served as a controlled system to dissect ligand-receptor interactions. The dual-targeting LNPs (DOPE(GPR77/CD10)) exhibited significantly higher cellular uptake in receptor-positive CHO cells compared to single-targeted or non-targeted formulations, demonstrating synergistic binding efficacy. These LNPs also showed excellent drug encapsulation and prolonged circulation. In a CHO xenograft model, dual-targeting LNPs achieved higher tumor accumulation than non-targeted controls, with minimal off-target organ retention. Biosafety assessments confirmed negligible hemolysis and no hepatorenal toxicity. While this study focused on mechanistic validation in a simplified model, our findings establish a generalizable platform for dual-receptor targeting, with potential applications in stromal or tumor cell-specific drug delivery.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100291"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Insights into the world of edible vaccines: From lab to reality 洞察可食用疫苗的世界:从实验室到现实
IF 3.6
Current Research in Biotechnology Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100290
Baveesh Pudhuvai , Bhupendra Koul , Awdhesh Kumar Mishra
{"title":"Insights into the world of edible vaccines: From lab to reality","authors":"Baveesh Pudhuvai ,&nbsp;Bhupendra Koul ,&nbsp;Awdhesh Kumar Mishra","doi":"10.1016/j.crbiot.2025.100290","DOIUrl":"10.1016/j.crbiot.2025.100290","url":null,"abstract":"<div><div>Edible vaccines have emerged as a groundbreaking advancement in the field of immunization, offering a unique and promising approach to combat infectious diseases. These are subunit vaccines wherein plants are genetically engineered to synthesise specific proteins to induce disease resistance. This idea of plant-based edible vaccines (PBVs) shall prove to be a boon for society, in the health sector as they are affordable, simple to administer and store. The use of edible vaccines has demonstrated a boost in mucosal and systemic immunity. Plants like potato, banana, lettuce, corn, soybean, rice, and legumes have been utilized for this application and have resulted in reduced diseases such as cholera, hepatitis, measles, diarrhoea. This review provides an overview of edible vaccines, exploring their development, advantages, challenges, and future potential. This further explores the processes involved in transforming plants using both direct and indirect gene delivery methods and provides an in-depth analysis of the background and clinical evidence for each plant candidate. Additionally, it also highlights the scientific advancements, ethical considerations, regulatory aspects, and potential impact of edible vaccines on global health and shall benefit researchers globally, in the field of developmental therapeutics, alternative medicine, and green chemistry.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100290"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sustainable remediation of persistent organic Pollutants: A review on Recent innovative technologies 持久性有机污染物的可持续修复:最新创新技术综述
IF 3.6
Current Research in Biotechnology Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100293
Fatihu Kabir Sadiq , Abdulalim Ahovi Sadiq , Tiroyaone Albertinah Matsika , Barikisu Ahuoyiza Momoh
{"title":"Sustainable remediation of persistent organic Pollutants: A review on Recent innovative technologies","authors":"Fatihu Kabir Sadiq ,&nbsp;Abdulalim Ahovi Sadiq ,&nbsp;Tiroyaone Albertinah Matsika ,&nbsp;Barikisu Ahuoyiza Momoh","doi":"10.1016/j.crbiot.2025.100293","DOIUrl":"10.1016/j.crbiot.2025.100293","url":null,"abstract":"<div><div>Persistent organic pollutants (POPs) are harmful chemicals that remain in the environment for a long time due to their resistance to degradation. They represent a significant challenge to sustainable development due to their enduring presence and potential for bioaccumulation in the environment. This review examines emerging technologies for POP remediation, assessing conventional methods while emphasizing the potential of innovative approaches. Advanced technologies, including nanotechnology and advanced oxidation processes (AOPs), show potential for effective, cost-efficient and environmentally friendly POP degradation. Among AOPs, methods such as UV/H<sub>2</sub>O<sub>2</sub>, Fenton’s reaction, photo-Fenton, UV/Ozone, and photocatalysis with semiconductors like TiO<sub>2</sub> are particularly effective in breaking down POPs into less harmful substances. Nanotechnology-based methods provide high efficiency, selectivity, and reduced environmental impact, while photocatalysis offers a sustainable and eco-friendly approach to POP degradation. To enhance remediation effectiveness, integrating innovative materials, optimized processes, and artificial intelligence (AI) is crucial. Advancements in eco-friendly nanomaterials, hybrid AOPs, and AI-driven process optimization can significantly improve degradation efficiency, selectivity, and sustainability. Future research should focus on scaling up these technologies, improving cost-effectiveness, and incorporating AI-powered smart monitoring systems for long-term environmental management. A holistic and sustainable remediation strategy must also align with circular economy principles, promote eco-friendly innovations, and address the influence of climate change on POP behavior. By fostering interdisciplinary research, policy support, and international collaboration, the development of accessible and sustainable remediation solutions can be accelerated, contributing to a cleaner environment and a healthier future for all.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100293"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic diversity and population structure of Cnaphalocrocis medinalis across India and South Asia: Insights from COI and ITS2 gene analyses 印度和南亚稻纵卷叶蝉的遗传多样性和种群结构:来自COI和ITS2基因分析的见解
IF 3.6
Current Research in Biotechnology Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100281
Soumya Shephalika Dash , Prasanthi Golive , C. Parameswaran , Prakash Chandra Rath , Hirak Chatterjee , Arup Kumar Mukherjee , Partha Sarathi Tripathy , Anjan Kumar Nayak , Sucharita Mohapatra , Bijay Kumar Behera , Shyamaranjan Das Mohapatra
{"title":"Genetic diversity and population structure of Cnaphalocrocis medinalis across India and South Asia: Insights from COI and ITS2 gene analyses","authors":"Soumya Shephalika Dash ,&nbsp;Prasanthi Golive ,&nbsp;C. Parameswaran ,&nbsp;Prakash Chandra Rath ,&nbsp;Hirak Chatterjee ,&nbsp;Arup Kumar Mukherjee ,&nbsp;Partha Sarathi Tripathy ,&nbsp;Anjan Kumar Nayak ,&nbsp;Sucharita Mohapatra ,&nbsp;Bijay Kumar Behera ,&nbsp;Shyamaranjan Das Mohapatra","doi":"10.1016/j.crbiot.2025.100281","DOIUrl":"10.1016/j.crbiot.2025.100281","url":null,"abstract":"<div><div>Rice leaffolder, <em>Cnaphalocrocis medinalis</em>, a major pest of rice, poses a significant threat to crop yield across Asia. This is why the spread of this pest across Southeast Asia needs to be studied to understand its population dynamics, genetic diversity, and potential impact on regional rice cultivation. In this study, the genetic diversity and population structure of <em>C. medinalis</em> were examined using mitochondrial <em>COI</em> and nuclear <em>ITS2</em> gene sequences collected from 15 Indian states and several South Asian countries. A total of 100 <em>COI</em> and 63 <em>ITS2</em> sequences were analyzed, including new and publicly available data on NCBI, to assess nucleotide diversity, haplotype variation, and phylogenetic relationships. The DNA extraction, PCR amplification, sequencing, and bioinformatics analyses were done. Results revealed higher haplotype and nucleotide diversity in Indian populations compared to other Asian regions, indicating genetic variations and potential historical bottlenecks followed by population expansions. Median-joining haplotype networks showed widespread haplotypes, suggesting gene flow, while region-specific haplotypes indicated localized adaptation. AMOVA highlighted substantial genetic differentiation among populations for <em>COI</em> (62.51% variation), whereas <em>ITS2</em> showed moderate differentiation (43.86%). Phylogenetic analyses identified polyphyletic and monophyletic clades, emphasizing complex population dynamics. Neutrality tests and mismatch distributions suggested demographic expansions in Indian populations. These findings underscore the evolutionary history, possible migration pattern and genetic structure of <em>C. medinalis</em>, providing insights into pest management strategies. Future research should explore environmental and anthropogenic factors driving genetic differentiation to enhance integrated pest management practices, ensuring sustainable rice production across diverse agro-climatic regions.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100281"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143806872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A systematic review on Indian Acacia species 文章题目印度金合欢种的系统综述
IF 3.6
Current Research in Biotechnology Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100274
Bharat Singh , Jagdish Prasad , Ram Avtar Sharma
{"title":"A systematic review on Indian Acacia species","authors":"Bharat Singh ,&nbsp;Jagdish Prasad ,&nbsp;Ram Avtar Sharma","doi":"10.1016/j.crbiot.2025.100274","DOIUrl":"10.1016/j.crbiot.2025.100274","url":null,"abstract":"<div><div>Acacia plants are evergreen, perennial, short, flat-crowned to long and straight-bole trees. A total of 34 species of Acacia genus are distributed in different states of India. Acacia plants are used in the treatment of chronic dysentery, diarrhoea, throat troubles, conjunctivitis and stomatitis. The critical data of ethnopharmacological properties, phytochemical and pharmacological attributes of 27 species were obtained through extensive review of relevant reference books, M. Sc./M. Tech dissertations, PhD theses, Web of Science, PubMed, Google Scholar, ScienceDirect, Wiley, Taylors &amp; Francis, Bentham, Thieme, Springer, and eFloras. The flavone glycosides, flavonoids, lignans, anthraquinones, monoterpenes, and alkaloids have been identified from 20 species of Indian Acacia species. Indian Acacia species possess antimicrobial, antidiabetic, antiarthritic, antistress, anti-inflammatory, antioxidant, anticancer, and wound healing properties. However, various reports are available in literature on phytochemistry and biological properties of 27 Indian Acacia species but, still 7 species have not been evaluated for their pharmacological studies. The review discusses updated knowledge of ethnopharmacological applications, phytochemical and pharmacological attributes, and pharmacokinetic profiles of Indian Acacia species.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100274"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143136675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Curcumin dietary supplementation enhances serum carbohydrate-degrading enzyme activities in buffaloes 饲粮中添加姜黄素可提高水牛血清碳水化合物降解酶的活性
IF 3.6
Current Research in Biotechnology Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100276
Maima Matin , Natalia Ksepka , Kamil Wysocki , Michel-Edwar Mickael , Alpana Pande , Jarosław Olav Horbańczuk , Artur Jóźwik , Olga Adamska , Artur Stolarczyk , Laszlo Barna Iantovics , Yordanka Ilieva , Pencho Penchev , Atanas G. Atanasov
{"title":"Curcumin dietary supplementation enhances serum carbohydrate-degrading enzyme activities in buffaloes","authors":"Maima Matin ,&nbsp;Natalia Ksepka ,&nbsp;Kamil Wysocki ,&nbsp;Michel-Edwar Mickael ,&nbsp;Alpana Pande ,&nbsp;Jarosław Olav Horbańczuk ,&nbsp;Artur Jóźwik ,&nbsp;Olga Adamska ,&nbsp;Artur Stolarczyk ,&nbsp;Laszlo Barna Iantovics ,&nbsp;Yordanka Ilieva ,&nbsp;Pencho Penchev ,&nbsp;Atanas G. Atanasov","doi":"10.1016/j.crbiot.2025.100276","DOIUrl":"10.1016/j.crbiot.2025.100276","url":null,"abstract":"<div><div>Curcumin, the major bioactive compound found in turmeric (<em>Curcuma longa</em>) is a subject of intensive research, due to its multiple bioactivities. Previous studies have investigated metabolic effects of curcumin in humans or established animal research models such as mice, but studies with farm animals have been scarce. In this work, we aimed to study the effects of curcumin supplementation in Bulgarian Murrah buffaloes (<em>Bubalus bubalis</em>), on the serum activities of seven important carbohydrate-metabolizing enzymes, β-glucuronidase, α-galactosidase, α-glucosidase, β-glucosidase, β-galactosidase, N-acetyl-hexosaminidase, and mannosidase. Curcumin was supplemented at a dose of 50 g per animal, per day, and serum activities of the studied enzymes were determined at the start (day 0), middle (day 14), and end (day 28) of the supplementation period. A tendency for increased activity of all studied enzymes was observed upon supplementation with curcumin, with the activity levels of five of the enzymes displaying statistically significant upregulation at day 28 (β-glucuronidase, α-galactosidase, β-galactosidase, N-acetyl-hexosaminidase, and mannosidase). These upregulated activities are in general indicative of increased carbohydrates turnover and detoxification processes, and might provide clues for a better understanding of the mechanisms of action of curcumin <em>in vivo</em>, as well as for novel approaches to rationally impact animal or human health and wellbeing through targeted supplementation.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100276"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143136749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Neurotensin(8-13) analogs targeting NTS1 and NTS2 receptors: A comparative in vitro and molecular modeling study 靶向NTS1和NTS2受体的神经紧张素(8-13)类似物:体外和分子模型的比较研究
IF 3.6
Current Research in Biotechnology Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100298
Kiril Kirilov , Maria Ponticelli , Toni Kühl , Harald Hübner , Maya G. Georgieva , Matthias Vogel , Aneliya A. Balacheva , Bodo Haas , Tamara I. Pajpanova , Maima Matin , Luigi Milella , Peter Gmeiner , Diana Imhof , Nikolay T. Tzvetkov
{"title":"Neurotensin(8-13) analogs targeting NTS1 and NTS2 receptors: A comparative in vitro and molecular modeling study","authors":"Kiril Kirilov ,&nbsp;Maria Ponticelli ,&nbsp;Toni Kühl ,&nbsp;Harald Hübner ,&nbsp;Maya G. Georgieva ,&nbsp;Matthias Vogel ,&nbsp;Aneliya A. Balacheva ,&nbsp;Bodo Haas ,&nbsp;Tamara I. Pajpanova ,&nbsp;Maima Matin ,&nbsp;Luigi Milella ,&nbsp;Peter Gmeiner ,&nbsp;Diana Imhof ,&nbsp;Nikolay T. Tzvetkov","doi":"10.1016/j.crbiot.2025.100298","DOIUrl":"10.1016/j.crbiot.2025.100298","url":null,"abstract":"<div><div>The simultaneous activation of both neurotensin type 1 and 2 receptors (NTS1R and NTS2R) through the neuronal peptide neurotensin (NT), activating the dopamine (DA) release and DA signaling within the dopaminergic system in the brain, suggest that NTS1R/NTS2R dual-specific NT analogs may represent an attractive tool in the treatment of Parkinson’s disease (PD) and/or other related conditions. Herein, we report <em>in silico</em> exploration of NTS1R and NTS2R driven by <em>in vitro</em> pharmacological evaluation of the linear hexapeptide NT analogs <strong>3</strong> (sequence Lys<sup>8</sup>-Cav<sup>9</sup>-Pro<sup>10</sup>-Tyr<sup>11</sup>-Ile<sup>12</sup>-Leu<sup>13</sup>) and <strong>6</strong> (Arg<sup>8</sup>-Cav<sup>9</sup>-Pro<sup>10</sup>-Tyr<sup>11</sup>-Ile<sup>12</sup>-Leu<sup>13</sup>), both active towards the human NTS1R and NTS2R. Compared to the parent peptide NT(8–13) (<strong>2</strong>), compounds <strong>3</strong> and <strong>6</strong> showed improved <em>in vitro</em> human plasma stability and BBB permeability. Moreover, <em>in silico</em> ADMET evaluation indicated that both NT-analogs have strong pharmacological properties combined with good safety profiles, highlighting their potential for further structural improvements. Furthermore, we applied an AI-based approach to generate the homology models of hNTS1R and hNTS2R, followed by MD simulations of their ligand-free state and molecular docking in order to estimate the most probable protein–ligand complexes of peptides <strong>3</strong> and <strong>6</strong>. Binding interaction/affinity analysis of the best-ranked docking modes, obtained with selected time-frames from the respective MD trajectories, suggest that the receptor activation occurs via a ligand-receptor binding into the initial “entry” conformation of hNTS1R and hNTS2R. This assumption is supported by additional HYDE analysis confirming the binding affinities of peptides <strong>3</strong> and <strong>6</strong> towards hNTS1R and hNTS2R obtained by radioligand binding experiments. The reported study may serve as a ready-to-use <em>in silico</em> approach for further development of therapeutic options against PD and potentially other neurological disorders.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100298"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144089151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Life cycle and environmental impact assessment of vegetation-activated sludge process (V-ASP) for decentralized wastewater treatment 用于分散式污水处理的植被活性污泥法(V-ASP)的生命周期和环境影响评估
IF 5.6
Current Research in Biotechnology Pub Date : 2024-01-01 DOI: 10.1016/j.crbiot.2023.100172
Pu Li , Xiaomeng Hu , Jiajia Yuan , Feiyun Sun , Pengfei Li , Wenyi Dong , Erdeng Du , Mingguo Peng
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