Current Research in Biotechnology最新文献

{"title":"Insights into the world of edible vaccines: From lab to reality","authors":"Baveesh Pudhuvai ,&nbsp;Bhupendra Koul ,&nbsp;Awdhesh Kumar Mishra","doi":"10.1016/j.crbiot.2025.100290","DOIUrl":"10.1016/j.crbiot.2025.100290","url":null,"abstract":"<div><div>Edible vaccines have emerged as a groundbreaking advancement in the field of immunization, offering a unique and promising approach to combat infectious diseases. These are subunit vaccines wherein plants are genetically engineered to synthesise specific proteins to induce disease resistance. This idea of plant-based edible vaccines (PBVs) shall prove to be a boon for society, in the health sector as they are affordable, simple to administer and store. The use of edible vaccines has demonstrated a boost in mucosal and systemic immunity. Plants like potato, banana, lettuce, corn, soybean, rice, and legumes have been utilized for this application and have resulted in reduced diseases such as cholera, hepatitis, measles, diarrhoea. This review provides an overview of edible vaccines, exploring their development, advantages, challenges, and future potential. This further explores the processes involved in transforming plants using both direct and indirect gene delivery methods and provides an in-depth analysis of the background and clinical evidence for each plant candidate. Additionally, it also highlights the scientific advancements, ethical considerations, regulatory aspects, and potential impact of edible vaccines on global health and shall benefit researchers globally, in the field of developmental therapeutics, alternative medicine, and green chemistry.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100290"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable remediation of persistent organic Pollutants: A review on Recent innovative technologies","authors":"Fatihu Kabir Sadiq ,&nbsp;Abdulalim Ahovi Sadiq ,&nbsp;Tiroyaone Albertinah Matsika ,&nbsp;Barikisu Ahuoyiza Momoh","doi":"10.1016/j.crbiot.2025.100293","DOIUrl":"10.1016/j.crbiot.2025.100293","url":null,"abstract":"<div><div>Persistent organic pollutants (POPs) are harmful chemicals that remain in the environment for a long time due to their resistance to degradation. They represent a significant challenge to sustainable development due to their enduring presence and potential for bioaccumulation in the environment. This review examines emerging technologies for POP remediation, assessing conventional methods while emphasizing the potential of innovative approaches. Advanced technologies, including nanotechnology and advanced oxidation processes (AOPs), show potential for effective, cost-efficient and environmentally friendly POP degradation. Among AOPs, methods such as UV/H₂O₂, Fenton’s reaction, photo-Fenton, UV/Ozone, and photocatalysis with semiconductors like TiO₂ are particularly effective in breaking down POPs into less harmful substances. Nanotechnology-based methods provide high efficiency, selectivity, and reduced environmental impact, while photocatalysis offers a sustainable and eco-friendly approach to POP degradation. To enhance remediation effectiveness, integrating innovative materials, optimized processes, and artificial intelligence (AI) is crucial. Advancements in eco-friendly nanomaterials, hybrid AOPs, and AI-driven process optimization can significantly improve degradation efficiency, selectivity, and sustainability. Future research should focus on scaling up these technologies, improving cost-effectiveness, and incorporating AI-powered smart monitoring systems for long-term environmental management. A holistic and sustainable remediation strategy must also align with circular economy principles, promote eco-friendly innovations, and address the influence of climate change on POP behavior. By fostering interdisciplinary research, policy support, and international collaboration, the development of accessible and sustainable remediation solutions can be accelerated, contributing to a cleaner environment and a healthier future for all.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100293"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity and population structure of Cnaphalocrocis medinalis across India and South Asia: Insights from COI and ITS2 gene analyses","authors":"Soumya Shephalika Dash ,&nbsp;Prasanthi Golive ,&nbsp;C. Parameswaran ,&nbsp;Prakash Chandra Rath ,&nbsp;Hirak Chatterjee ,&nbsp;Arup Kumar Mukherjee ,&nbsp;Partha Sarathi Tripathy ,&nbsp;Anjan Kumar Nayak ,&nbsp;Sucharita Mohapatra ,&nbsp;Bijay Kumar Behera ,&nbsp;Shyamaranjan Das Mohapatra","doi":"10.1016/j.crbiot.2025.100281","DOIUrl":"10.1016/j.crbiot.2025.100281","url":null,"abstract":"<div><div>Rice leaffolder, <em>Cnaphalocrocis medinalis</em>, a major pest of rice, poses a significant threat to crop yield across Asia. This is why the spread of this pest across Southeast Asia needs to be studied to understand its population dynamics, genetic diversity, and potential impact on regional rice cultivation. In this study, the genetic diversity and population structure of <em>C. medinalis</em> were examined using mitochondrial <em>COI</em> and nuclear <em>ITS2</em> gene sequences collected from 15 Indian states and several South Asian countries. A total of 100 <em>COI</em> and 63 <em>ITS2</em> sequences were analyzed, including new and publicly available data on NCBI, to assess nucleotide diversity, haplotype variation, and phylogenetic relationships. The DNA extraction, PCR amplification, sequencing, and bioinformatics analyses were done. Results revealed higher haplotype and nucleotide diversity in Indian populations compared to other Asian regions, indicating genetic variations and potential historical bottlenecks followed by population expansions. Median-joining haplotype networks showed widespread haplotypes, suggesting gene flow, while region-specific haplotypes indicated localized adaptation. AMOVA highlighted substantial genetic differentiation among populations for <em>COI</em> (62.51% variation), whereas <em>ITS2</em> showed moderate differentiation (43.86%). Phylogenetic analyses identified polyphyletic and monophyletic clades, emphasizing complex population dynamics. Neutrality tests and mismatch distributions suggested demographic expansions in Indian populations. These findings underscore the evolutionary history, possible migration pattern and genetic structure of <em>C. medinalis</em>, providing insights into pest management strategies. Future research should explore environmental and anthropogenic factors driving genetic differentiation to enhance integrated pest management practices, ensuring sustainable rice production across diverse agro-climatic regions.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100281"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143806872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Life cycle and environmental impact assessment of vegetation-activated sludge process (V-ASP) for decentralized wastewater treatment","authors":"Pu Li ,&nbsp;Xiaomeng Hu ,&nbsp;Jiajia Yuan ,&nbsp;Feiyun Sun ,&nbsp;Pengfei Li ,&nbsp;Wenyi Dong ,&nbsp;Erdeng Du ,&nbsp;Mingguo Peng","doi":"10.1016/j.crbiot.2023.100172","DOIUrl":"10.1016/j.crbiot.2023.100172","url":null,"abstract":"<div><p>The integrated Vegetation-Activated Sludge Process (V-ASP) has emerged as a promising solution for decentralized wastewater treatment, offering a unique combination of landscape vegetation with the traditional Anaerobic-Anoxic-Oxic process (AAO). The experiments displayed that V-ASP consistently demonstrated remarkable treatment performance, maintaining stable removal efficiency exceeding 90 % for COD, NH₄⁺-N, and TP. The vegetation growth is helpful for the removal of pollutants and the microbial community in the allocated vegetation root, while the bulk suspended sludge was changed significantly by using PCR test. To comprehensively evaluate the environmental footprint of V-ASP, a Life Cycle Assessment (LCA) was conducted. The mass balance calculation containing wastewater treatment performance, energy consumption, vegetation growth behavior, greenhouse gas (GHG) emissions, treated water, and sludge discharge was carried out to establish the life cycle inventories (LCI). SimaPro 9.0 software and the ReCiPe (H) midpoint impact assessment method were employed, which revealed that the V-ASP system boasts low GHG emissions and freshwater eutrophication potential compared to the traditional AAO process. In essence, this study provides a comprehensive understanding of the V-ASP system, especially the environmental impacts, guiding its potential for sustainable decentralized sewage treatment applications.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100172"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262823000540/pdfft?md5=b199791f2cdc6d6f47b7448ee381f95e&pid=1-s2.0-S2590262823000540-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139023335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green extract surface-coated electrospun cellulose nanofibers as an efficient, reversible, and reusable smart colorimetric sensor for real-time monitoring of chicken freshness","authors":"Muhammad Asim Iqbal ,&nbsp;Sabeen Gohar ,&nbsp;Yoshinori Yabuta ,&nbsp;Liang Fanghua ,&nbsp;Wang Ling ,&nbsp;Muhammad Farooq ,&nbsp;Muhammad Nauman Sarwar ,&nbsp;Gopiraman Mayakrishnan ,&nbsp;Ick Soo Kim","doi":"10.1016/j.crbiot.2024.100192","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100192","url":null,"abstract":"<div><p>Smart colorimetric sensor for the naked-eye detection of food freshness is considered as the most attractive tool in food safety. Herein, we prepared curcumin (CUR) coated electrospun regenerated cellulose nanofiber (RCA) composites (CUR-Cot), to monitor the real-time spoilage of raw chicken. The physicochemical properties of the CUR-Cot sensor were completely studied. The surface modification, morphology, crystalline nature, and thermal stability of CUR-Cot were investigated by using various spectral, analytical, and microscopic techniques. Based on the results, the successful coating of CUR on the surface of RCA was confirmed. Interestingly, the CUR-Cot showed a significant change in total color difference value (ΔE, 0 days − 0.0–14.93, after 1 day – 14.93–23.64, after 2 days – 23.64–44.78, after 3 days- 44.78–55.22, and after 4 days – 55.22–60.96, detectable by the naked eye) in the real-time monitoring for chicken freshness. In addition, the present CUR-Cot smart colorimetric sensor is reversible with change in pH, and the reversed sensor can be reused. Furthermore, the hydrophobic nature of the CUR-Cot was confirmed by water contact angle analysis (WCA, contact angle of 90 ± 3.00), which increases its application and adaptability. Good antibacterial, barrier, and optical properties of the present CUR-Cot were also found. Overall, the results confirm that the usage of CUR-Cot sensor can be highly efficient, user-friendly, and non-destructive for the real-time monitoring of chicken freshness.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100192"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000182/pdfft?md5=273c453ff8f94e676def0bd2a303fe31&pid=1-s2.0-S2590262824000182-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140042235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of a prognostic model based on cuproptosis and ferroptosis-related genes in patients with head and neck squamous cell carcinoma","authors":"Yingjie Wu ,&nbsp;Tao Zhou ,&nbsp;Juanjuan Hu ,&nbsp;Lisha Wu ,&nbsp;Yao Hu ,&nbsp;Liuqing Zhou","doi":"10.1016/j.crbiot.2024.100253","DOIUrl":"10.1016/j.crbiot.2024.100253","url":null,"abstract":"<div><h3>Background</h3><p>Head and neck squamous cell carcinoma (HNSCC) is a common invasive malignant tumor that lacks powerful predictive or prognostic biomarkers. Ferroptosis and cuproptosis are two new forms of programmed cell death. Our study was aimed at constructing a prognostic model with a combination of cuproptosis and ferroptosis-related genes (CFRGs) for the early clinical detection of HNSCC.</p></div><div><h3>Methods</h3><p>We obtained the information of CFRGs, including the RNASeq data and corresponding clinical data in HNSCC patients from the TCGA and GEO databases. We assessed 28 CFRGs, and analyzed the relationship between those genes and their clinical features and prognosis of HNSCC. The consensus cluster analysis was employed to generate three CFRGclusters. Then, we investigated the association of molecular patterns and prognostic significance in these subtypes. The clinical indicators of the prognosis-related genes were identified and prognostic CFRG_score were constructed. We then built a predictive nomogram with confirmed consistency and reliability by calibration curve analysis. At last, we verified the expression of CFRGs in HNSCC tissues by qRT-PCR and immunohistochemical results.</p></div><div><h3>Results</h3><p>The DEGs were different between the normal and HNSCC tumor tissues and we screened out 28 CFRGs related to the prognosis in HNSCC. Associations between the clinical information and prognosis were found in the molecular subtypes related to prognosis. We utilized enrichment analysis of the differential genes and showed that those DEGs were mostly enriched in the biological processes associated with the pathways of neurodegeneration-multiple diseases, Alzheimer disease, Prion disease, Parkinson disease and Amyotrophic lateral sclerosis. CFRG_score was established to predict the survival of HNSCC patients and found that higher CFRG_score suggested favorable OS for patients, indicating the prediction of better prognosis. Moreover, we created highly reliable nomogram which could predict well for the expected prognosis. In addition, we confirmed that the expression of EGFR, VEGFA, HSPA5, SLC3A2, CAV1 and CD44 were consistent with qRT-PCR and immunohistochemical analysis in HNSCC tissues by qRT-PCR.</p></div><div><h3>Conclusions</h3><p>This prognostic model based on prognostic differential CFRG_score is strongly related to clinical characteristics, prognosis, and therapy in HNSCC patients and could be used as a promising tool which is dedicated to guiding the treatment of HNSCC.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"8 ","pages":"Article 100253"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000790/pdfft?md5=3b344592f4a8895e4773a2d0fd9babcf&pid=1-s2.0-S2590262824000790-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142232291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review: Anti-obesity drug discovery from natural plant metabolites and endogenous peptides","authors":"Xiaomu Zhu ,&nbsp;Dongdong Wang ,&nbsp;Atanas G. Atanasov","doi":"10.1016/j.crbiot.2024.100259","DOIUrl":"10.1016/j.crbiot.2024.100259","url":null,"abstract":"<div><div>Obesity is a condition of abnormal or excessive body fat accumulation. It is a chronic and degenerative disease that is a global health problem and causes large societal and economic burdens. However, until now, long-term pharmacotherapies to lower body weight to normal along with suitable tolerability and fewer side effects have remained a challenge. Natural products from plants are a promising source for new lead structures for drug discovery. Additionally, endogenous natural peptides have attracted increasing attention for fighting obesity recently. In this review, we first summarize recent findings on promising signaling/targets for managing body weight, including inhibition of appetite, nutrient absorption and energy expenditure. We also discuss pure natural compounds (such as betulinic acid) and endogenous peptides (such as GLP1, amylin, GIP, ghrelin) and their derivatives (such as orlistat) with anti-obesity effects.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"8 ","pages":"Article 100259"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142526113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic comparisons of preparative protocols to generate various types of stone-related crystals for in vitro study of renal calculi","authors":"Niracha Koeipudsa,&nbsp;Paleerath Peerapen,&nbsp;Visith Thongboonkerd","doi":"10.1016/j.crbiot.2024.100239","DOIUrl":"10.1016/j.crbiot.2024.100239","url":null,"abstract":"<div><p><em>In vitro</em> studies of stone-related crystals and crystals-cell interactions have been extensively done to investigate cellular, molecular and pathogenic mechanisms leading to renal calculi. Effective preparation of various types of stone-related crystals is thus crucial for such studies. Nevertheless, various protocols for preparing these stone-related crystals were scatteredly reported without comparative analysis of their efficacies and yields. Herein, we systematically compared our protocols (with the suffix “-Si”) for preparing calcium oxalate (CaOx) monohydrate (COM), CaOx dihydrate (COD), magnesium ammonium phosphate (struvite), uric acid (UA), calcium phosphate dihydrate (brushite), hydroxyapatite (HAP), and calcium carbonate (CaCO₃) crystals with other protocols published previously. The morphological evaluation revealed that our protocols provided the most homogeneous and most typical monoclinic prismatic, bipyramidal, coffin lid and rectangle shapes of COM, COD, struvite and UA crystals, respectively. There were comparable morphological results for brushite, HAP and CaCO₃ crystals generated by different protocols. Our protocols provided the greatest yield for generating brushite crystals but with lower yields for others. Chemical analysis by Fourier transform infrared (FTIR) spectroscopy revealed comparable results among different protocols to generate each crystal type. In summary, all these protocols can be used to generate each crystal type. But our protocols offer the best quality, in terms of homogeneity and typical shape, for generating COM, COD, struvite and UA crystals.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"8 ","pages":"Article 100239"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000650/pdfft?md5=dd85f9bd8d02cea1de220d5e9b696334&pid=1-s2.0-S2590262824000650-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141852088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the anti-inflammatory and anti-arthritis effects of fucoidan from a brown seaweed","authors":"Preethy P. Raj ,&nbsp;Rajesh Kanna Gopal ,&nbsp;Elumalai Sanniyasi","doi":"10.1016/j.crbiot.2024.100220","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100220","url":null,"abstract":"<div><p>Severe inflammation in joints caused by the detrimental effects of the immune system is termed Rheumatoid arthritis. The unconstrained proliferation of immune cells and pro-inflammatory cytokines deteriorates Synovium which secretes synovial fluid to lubricate joints and cartilage. Non-steroidal anti-inflammatory drugs (NSAIDs) are the only therapeutics for treating rheumatoid arthritis, and long-term intake causes serious side effects on the organs. Fucoidan, a sulfated polysaccharide found on the cell walls of brown algae shows bioactive potential. In our study, fucoidan was extracted from <em>Padina pavonica</em> (PD), <em>Stoechospermum marginatum</em> (StM), <em>Spatolossum macrodontum</em> (SpM), <em>Dictyota bartayresiana</em> (DD), and <em>Turbinaria decurrens</em> (TD) and evaluated for anti-inflammatory and anti-arthritis activities. Fucoidan was extracted and evaluated for anti-inflammatory activity <em>in vitro</em> using RAW 264.7 macrophage cell lines, followed by <em>in vivo</em> anti-arthritis activity on Wistar male rats. Nitric oxide suppression was comparatively high in fucoidan from TD (IC₅₀ − 12.93 µg/mL). Purified fucoidan from TD, significantly reduced inflammation, size of paw edema, downregulated proinflammatory cytokines (IL-6, IL-1β, TNF-α), and upregulated anti-inflammatory cytokine (IL10) in CFA-induced arthritis in Wistar male rats. Biochemical parameters like SOD, CAT, GSH, GPX, and GST and haematological parameters like total-protein, albumin, haemoglobin, and RBC were upregulated, and other parameters like urea, uric acid, creatinine, bilirubin, SGOT, SGPT, ALP, WBC, ESR, RF, and CRP were downregulated. Histopathology of the liver, kidney, and ankle joints reveals that fucoidan intake restrained inflammation and tissue damage. Therefore, fucoidan extracted from TD is a potential candidate for the treatment of rheumatoid arthritis.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100220"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000467/pdfft?md5=e3746c4e2d04e126ed16f7ed27d36772&pid=1-s2.0-S2590262824000467-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140901381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selection of aptamer for creatine Kinase-MM as a marker for muscular dystrophy and myocardial infarction","authors":"Albert M Liao ,&nbsp;Shailaja Agrawal ,&nbsp;James Cory Benson ,&nbsp;G. Thomas Caltagirone","doi":"10.1016/j.crbiot.2024.100229","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100229","url":null,"abstract":"<div><p>Creatine kinase-MM (CK-MM) is a relatively muscle-specific enzyme with a plasma half-life of approximately 2 h. Total creatine kinase elevation is evident in several conditions associated with acute muscle injury, severe muscular exertion, and myocardial infarction. The presence of a large amount of the CK-MM enzyme in blood serum is a biomarker of muscular injuries and cardiac assault. In this study, we developed a structure-switching aptamer that can be immobilized on a sensor to detect CK-MM. CK-BB was used as a counter-target to ensure the specific targeting of CK-MM. Melting-Off SELEX was employed to develop aptamers exhibiting significant structural changes on binding. The selected aptamer shows a high affinity towards CK-MM with a K_d value of 14.7 nM.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100229"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000558/pdfft?md5=f3b0eaa258043b204aeb1269ea2335fb&pid=1-s2.0-S2590262824000558-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141240229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}