Current Research in Biotechnology最新文献

{"title":"Bridging the translational divide: nanotechnological platforms for overcoming endolysin limitations against antibiotic-resistant infections","authors":"Marjan Rashidan,&nbsp;Roya Moravej,&nbsp;Mina Owrang","doi":"10.1016/j.crbiot.2026.100410","DOIUrl":"10.1016/j.crbiot.2026.100410","url":null,"abstract":"<div><div>Antibiotic resistance is a major global health threat, driving the need for alternative antimicrobial strategies. Bacteriophage-derived endolysins are promising agents due to their rapid and specific bactericidal activity, but their clinical application is limited by poor stability, short half-life, and inability to penetrate Gram-negative outer membranes. Nanotechnology-based delivery systems offer potential solutions to these limitations. This review summarizes recent advances in polymeric nanoparticles, liposomal systems, metal-based nanoparticles, and self-assembling peptides for enhancing endolysin efficacy. These platforms improve stability, bioavailability, and membrane penetration while enabling controlled and targeted delivery. Despite promising preclinical outcomes, clinical translation remains limited due to regulatory, manufacturing, and immunological challenges. Addressing these barriers is essential for advancing nanotechnology-enhanced endolysins toward clinical application.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"12 ","pages":"Article 100410"},"PeriodicalIF":6.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148477292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meta-analysis reveals significant microbial community differentiation between plastisphere and surrounding water in aquatic environments","authors":"Qu-Yi Zhao,&nbsp;Bin-Yuan Gao,&nbsp;Ke Shi,&nbsp;Shu-Hao Du,&nbsp;Bin Liang","doi":"10.1016/j.crbiot.2026.100415","DOIUrl":"10.1016/j.crbiot.2026.100415","url":null,"abstract":"<div><div>Aquatic microplastics harbor a unique biofilm termed the plastisphere. However, study-level quantitative synthesis of paired plastisphere–surrounding water differences in microbial diversity, community composition, and immigration-related assembly parameters remains limited. We performed a meta-analysis of 15 16S rRNA gene sequencing studies (1091 samples) comparing plastisphere and surrounding water microbiomes. Community α-diversity indices showed a non-significant tendency to be higher in the plastisphere than in the surrounding water. Importantly, β-diversity analysis revealed consistent and significant differentiation between habitat types, with habitat type explaining a moderate but statistically significant proportion of compositional variance. Taxonomic analysis identified enriched taxa dominated by Proteobacteria, along with depleted genera primarily affiliated with Actinobacteria and Bacteroidetes. A random forest classifier distinguished the two habitats with excellent performance (accuracy: 96%). Neutral community modeling revealed that the immigration rate (m) was significantly lower in the plastisphere (pooled lnRR = −1.39, 95% CI: −2.09 to −0.70), indicating reduced dispersal from the regional pool. These findings do not support a universal reduction in microbial α-diversity on microplastic surfaces and instead indicate that α-diversity responses are strongly context dependent, where estimated reduction in immigration and lower stochasticity may contribute to distinct and variable microbial assemblages. This study highlights the necessity of accounting for contextual drivers and assembly mechanisms in assessing ecological impacts of microplastic pollution.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"12 ","pages":"Article 100415"},"PeriodicalIF":6.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148770258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A bioactive enriched fraction derived from malts and hops, enhances keratinocyte antioxidant defenses and modulates psoriasis-like inflammation","authors":"Eva Adabbo,&nbsp;Giacomo De Palma,&nbsp;Idolo Tedesco,&nbsp;Maria Recine,&nbsp;Gianluca Picariello,&nbsp;Francesco Siano,&nbsp;Fabrizio Tarricone,&nbsp;Carla Cicala,&nbsp;Carmela Spagnuolo,&nbsp;Gian Luigi Russo","doi":"10.1016/j.crbiot.2026.100416","DOIUrl":"10.1016/j.crbiot.2026.100416","url":null,"abstract":"<div><div>A (poly)phenol enriched fraction, named Aliophen-XP, was obtained from a patented malt and hop derived formulation (Aliophen®). Here, we combined chemical profiling with in vitro and in vivo assays to evaluate its antioxidant and anti-inflammatory activity in models relevant to psoriasis. Aliophen-XP was obtained by protein/carbohydrate depletion and C18 solid phase extraction (35% acetonitrile) and characterized by HPLC UV FLD ESI Q TOF MS/MS and nano LC Q-Orbitrap MS/MS. The fraction displayed a high abundance of barley phenolamides, predominantly hordatines and their glycosylated derivatives, together with minor flavonoid C glycosides and malt derived peptides. Total (poly)phenols and antioxidant capacity were 36.30 ± 2.15 mg quercetin equivalents/g (dw) and 81.92 ± 9.06 mg Fe²⁺/g (dw), respectively. In HaCaT keratinocytes, Aliophen-XP was non cytotoxic across tested exposures, markedly reduced tert-butyl hydroperoxide–induced ROS, and suppressed TNFα stimulated expression of IL 1β, IL 6, and IL 8, with concordant reduction of IL 6 protein. Aliophen-XP also increased NQO1 protein levels. In C57BL/6 J mice, preventive topical application (0.8–1.6 mg mL-1, 30 min before imiquimod) attenuated psoriasiform disease, reducing ear thickening, erythema and scaling (PASI components) and lowering tissue IL 17 A levels.</div><div>The findings support further development of Aliophen-XP as a topical adjunct for psoriatic skin, including therapeutic onset regimens, formulation optimization, and fractionation guided mechanistic studies to define the active principles and pathways.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"12 ","pages":"Article 100416"},"PeriodicalIF":6.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148951596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated shotgun metagenomic and culture-based characterization of plant growth-promoting rhizobacteria associated with Sangyod Rice","authors":"Jannisuta Rangchai,&nbsp;Natthasap Moolika,&nbsp;Khunanon Chanasongkhram,&nbsp;Ponsit Sathapondecha,&nbsp;Jutarut Iewkittayakorn,&nbsp;Unitsa Sangket","doi":"10.1016/j.crbiot.2026.100427","DOIUrl":"10.1016/j.crbiot.2026.100427","url":null,"abstract":"<div><div>Sangyod rice (<em>Oryza sativa</em> L. var. <em>indica</em>) is a high-value traditional rice variety in southern Thailand. Organic farming practices may enhance rhizosphere microbial diversity and promote beneficial plant growth-promoting rhizobacteria (PGPR), but the microbial composition and functional potential of the Sangyod rice rhizosphere remain poorly understood. Long-read shotgun metagenomic sequencing (Oxford Nanopore) was used to characterize rhizosphere bacterial communities in organic and chemical cultivation systems. Six samples, comprising three samples the chemical cultivation system (C2, C3, and C4) and three from the organic cultivation system (O2, O3, and O4), were sequenced and analyzed individually. Culturable rhizobacteria were isolated and screened for plant growth-promoting traits, including potential nitrogen-fixing ability, phosphate-solubilizing potential and IAA-producing potential. Selected isolates were identified by 16S rRNA gene sequencing and evaluated for antagonistic interactions using a reciprocal agar disc diffusion assay. Shotgun metagenomic analysis identified Pseudomonadota and Actinomycetota as the predominant phyla, with <em>Streptomyces</em> as the most abundant genus across all samples. Alpha-diversity indices did not differ significantly between the cultivation systems. Isolate SSY16, closely related to <em>Streptomyces</em> sp., showed the highest phosphate-solubilizing index (PSI = 1.799 ± 0.088), while SSN34, closely related to <em>Bacillus</em> sp., showed IAA-producing potential (estimated IAA concentration: 38.96 μg mL⁻¹) and no detectable antagonism with SSY16 under the tested agar conditions. The integration of shotgun metagenomic analysis and culture-based screening enabled the characterization of the rhizosphere bacterial community and the identification of cultivable rhizobacteria with plant growth-promoting traits. SSY16 and SSN34 represent a preliminary candidate consortium requiring further validation in pot experiments.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"12 ","pages":"Article 100427"},"PeriodicalIF":6.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149199503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and pharmacological assessment of pyrrole carboxylic acid derivatives: unexpected pro-inflammatory action of a hexanoic analogue in rats","authors":"Hristina Zlatanova-Tenisheva ,&nbsp;Stanislava Vladimirova ,&nbsp;Yordan Todorov","doi":"10.1016/j.crbiot.2026.100372","DOIUrl":"10.1016/j.crbiot.2026.100372","url":null,"abstract":"<div><div>Pyrrole-based carboxylic acid derivatives are a promising scaffold in medicinal chemistry due to their potential anti-inflammatory and analgesic properties. In this study, we synthesized two series of novel pyrrole derivatives (BB and CC) and evaluated their <em>in vivo</em> antinociceptive and anti-inflammatory activity using male Wistar rats. Compounds were administered intraperitoneally at 40 mg/kg, with metamizole and diclofenac serving as reference drugs. Thermal nociception was assessed via the plantar test, and acute inflammation was induced by carrageenan-mediated paw edema. Reference drugs produced the expected analgesic and anti-inflammatory effects, confirming model reliability. In contrast, none of the BB or CC compounds significantly altered paw withdrawal latency or edema, indicating a lack of analgesic and anti-inflammatory efficacy. Notably, <strong>CC1</strong>, a hexanoic acid derivative, caused a significant increase in paw swelling at 3–4 h post-carrageenan, suggesting a pro-inflammatory effect. Structure-activity analysis revealed that variations in side-chain length and <em>para</em>-substitution on the phenyl ring did not enhance pharmacological activity. The absence of efficacy may be due to limited tissue penetration, insufficient target engagement, or metabolic factors, while the pro-inflammatory effect of <strong>CC1</strong> may involve local accumulation, reactive oxygen species generation, and activation of pro-inflammatory signaling pathways. These findings highlight critical limitations of the current pyrrole scaffold and emphasize the need for thorough <em>in vivo</em> assessment during drug development. Overall, while pyrrole carboxylic acids remain a chemically versatile platform, substantial structural optimization is required to achieve effective analgesic or anti-inflammatory activity.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"11 ","pages":"Article 100372"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147395194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel 2-aminobenzimidazolyl-1-hydrazones with antileukemic activity: Potent alternatives to imatinib and ibrutinib","authors":"Neda Anastassova ,&nbsp;Elena Stoyanova ,&nbsp;Denitsa Yancheva ,&nbsp;Tanya Dimova ,&nbsp;Miroslav Rangelov ,&nbsp;Svetla Todinova ,&nbsp;Maya Guncheva","doi":"10.1016/j.crbiot.2026.100378","DOIUrl":"10.1016/j.crbiot.2026.100378","url":null,"abstract":"<div><div>Chronic leukemias are serious, life-threatening blood malignancies that require long-term treatment. Current drug development focuses on precise, targeted therapies to overcome the limitations of conventional chemotherapeutics, such as low efficacy and adverse side effects. In this study, we synthesized a series of novel 2-aminobenzimidazolyl-1-hydrazones through a three-step reaction and structurally characterized the compounds. Their cytotoxic activity was evaluated in K562 cells (a model of chronic myeloid leukemia) and HG-3 cells (a model of chronic lymphocytic leukemia). The mechanism of cell death, apoptosis or necrosis, was assessed by flow cytometry after 24 h of treatment with the most active compounds at their respective IC₅₀ values. To evaluate potential hepatotoxicity during early-stage drug development, cytotoxicity was also tested in HepG2 cells, a widely used hepatic model. Among the four compounds tested, 3-(2-amino-1H-benzo[d]imidazol-1-yl)-N′-(2,3-dihydroxybenzylidene)propanehydrazide (<strong>4a</strong>) and 3-(2-amino-1H-benzo[d]imidazol-1-yl)-N′-(2-hydroxy-4-methoxybenzylidene)propanehydrazide (<strong>4b</strong>) showed the most potent cytotoxic effects, matching or exceeding the activity of clinically used drugs, imatinib and ibrutinib, in their respective cell line models.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"11 ","pages":"Article 100378"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147395317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking antimicrobial potential of microalgae on food-borne bacteria: A standardized framework and future directions","authors":"Delphine Rapp ,&nbsp;Dieke Schelvis ,&nbsp;Nigel P. French ,&nbsp;Maxence Plouviez","doi":"10.1016/j.crbiot.2025.100362","DOIUrl":"10.1016/j.crbiot.2025.100362","url":null,"abstract":"<div><div>Foodborne infections are a global challenge, costing billions annually through food losses, trade restrictions, and healthcare expenses. Growing concerns over chemical antimicrobials such as antibiotics, sanitizers, and disinfectants, have driven interest in sustainable bio-control strategies for food systems. Microalgae, which produce a plethora of biomolecules including carbohydrates, lipids, proteins, and various secondary metabolites, represent a promising source of antimicrobial compounds. Despite numerous reports demonstrating antimicrobial activity in microalgal extracts, no microalgae-derived antimicrobials have yet reached commercialization.</div><div>This review focuses on some microalgal species already produced at commercial scale, including those with GRAS status (e.g., <em>Chlorella vulgaris</em> and <em>Chlamydomonas reinhardtii</em>). As for other microalgae-based products (e.g., biofuel oil), successful antimicrobial production depends on identifying key species and strains, optimizing growth conditions, and refining harvesting, cell disruption, and extraction protocols. Although research in this area is expanding, further studies are needed to improve our understanding of antimicrobials synthesis and to assess how these factors influence antimicrobial activity. Commonly used antibacterial assays such as disc diffusion and microdilution have limitations that must be considered when evaluating the antimicrobial activity of microalgal extracts. Overall, inconsistencies in testing and reporting have hindered the clear identification of microalgae as sources of effective antimicrobials. This review proposes a framework for future extract preparation and antimicrobial assessment and discusses future prospects to enhance the discovery and yield of microalgal antimicrobials.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"11 ","pages":"Article 100362"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145925293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tailored bacterial co-cultures improve Tisochrysis lutea growth and nutrient profiles under xenic conditions: a new pathway to improve microalgal production","authors":"Tamára F. Santos ,&nbsp;Beatriz Simões ,&nbsp;Veronica Rossetto ,&nbsp;Hugo Pereira ,&nbsp;Inês B. Maia ,&nbsp;Marta Oliveira ,&nbsp;Aschwin Engelen ,&nbsp;João Navalho ,&nbsp;João Varela","doi":"10.1016/j.crbiot.2025.100361","DOIUrl":"10.1016/j.crbiot.2025.100361","url":null,"abstract":"<div><div>The marine haptophyte <em>Tisochrysis lutea</em> is a valuable source of high-value compounds, including polyunsaturated fatty acids like docosahexaenoic acid, and pigments (e.g., fucoxanthin). However, high production costs and variability remain major challenges for its large-scale application in aquaculture, pharmaceuticals, and biotechnology industries. Therefore, strategies to enhance biomass production and quality are actively explored. In natural environments, <em>T. lutea</em> establishes mutualistic interactions with bacteria to obtain essential nutrients such as vitamin B₁₂, yet the role of bacteria in industrial cultures remains poorly understood. In this study, 145 bacterial strains were isolated and taxonomically identified from industrial <em>T. lutea</em> cultures, with members of the class Gammaproteobacteria and Actinomycetia being the most prevalent. Forty isolates were screened individually in co-culture with <em>T. lutea</em> revealing strain-specific effects on growth and biochemical composition. Seven beneficial strains were used to design 21 tailored bacterial blends. Several consortia enhanced biomass production (up to 74 %) and increased key bioactive compounds, particularly methylcobalamin (up to 300 %). These findings demonstrate the potential of tailored bacterial consortia to enhance <em>T. lutea</em> productivity and nutritional quality under production-relevant xenic conditions, enabling strategic microbiome modulation for specific industrial goals.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"11 ","pages":"Article 100361"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145790661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient polyhydroxyalkanoates production by anoxic mixed microbial cultures in two-stage sequencing batch reactors: conceptual validation and microbial insights","authors":"Xueyi Ruan ,&nbsp;Tianyu Zou ,&nbsp;Fengyi She ,&nbsp;Jiacheng Tang ,&nbsp;Hanqi Zhang ,&nbsp;Wenkui Mi ,&nbsp;Hua Lin ,&nbsp;Xiaowu Huang","doi":"10.1016/j.crbiot.2026.100391","DOIUrl":"10.1016/j.crbiot.2026.100391","url":null,"abstract":"<div><div>Single-stage microbial production of polyhydroxyalkanoates (PHA) requests concurrent microbial selection, biomass maintenance, and intracellular polymer storage within the same unit, thereby hindering the attainment of high-level PHA storage. Two-stage sequencing batch reactors (SBRs), designated as A-SBR and B-SBR, were established to develop efficient PHA production by mixed microbial cultures (MMC). Our results showed that A-SBR had developed a continuous biomass with a stable PHA storage capacity of 31.1±6.4%, with a maximum PHA content of 44.3%. On this basis, B-SBR achieved efficient PHA storage of 63.2±10.7% during the steady state with a peak value of 86.8%, with continuously receiving the PHA-storing biomass from A-SBR. Correspondingly, the PHA yields of A-SBR and B-SBR were 0.2±0.1 and 0.5±0.1 g COD_PHA/g COD, respectively. Notably, B-SBR achieved a peak PHA yield of 0.8 g COD_PHA/g COD, promising its superior PHA production potency. The 16S rRNA gene amplicon sequencing revealed that the two-stage SBRs developed rich and distinct PHA-producing populations, which together occupied 37.9%−54.9% and 33.6%−62.3% of the total microbial populations in A-SBR and B-SBR, respectively. Notably, <em>Allobrachymonas</em> and <em>Paracoccus</em> dominated in A-SBR, and <em>Paracoccus</em>, <em>Allobrachymonas</em>, and <em>Thauera</em> dominated in B-SBR. Prediction of microbial functional profiles further validated the dominance of enzymes essential for PHA biosynthesis. This study was the first to validate the high efficiency of MMC-based anoxic PHA production in a two-stage SBR configuration, which promises high rate PHA production in a cost-saving manner owing to the anoxic operation mode and thus encourages further efforts on the development of MMC-based two-stage anoxic PHA production.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"11 ","pages":"Article 100391"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147991344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of in silico prediction model for standardized ileal digestibility of amino acids from protein sources","authors":"Takuya Kikuchi ,&nbsp;Masamichi Takeshita ,&nbsp;Yusuke Adachi ,&nbsp;Yoji Yamada","doi":"10.1016/j.crbiot.2026.100368","DOIUrl":"10.1016/j.crbiot.2026.100368","url":null,"abstract":"<div><div>Digestibility is an important factor in the nutritional evaluation of proteins because it determines the bioavailability of the amino acids derived from them. In this study, we developed a prediction model for standardized ileal digestibility (SID) of amino acids using previously reported pig SID datasets. The prediction was performed using the random forest model with the explanatory variables, including nutrients, protein types, cooking/processing, and other ingredients. Shapley additive explanations and feature importance analysis identified the factors of SID prediction and the correlation between digestibility and nutrient data. The predictability for pig SID data was compared between the prediction model and the reported digestibility data of an existing alternative method–<em>in vitro</em> INFOGEST digestion protocol. Finally, the digestible indispensable amino acid score (DIAAS) evaluation using predicted SID showed high accuracy compared with that using observed SID.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"11 ","pages":"Article 100368"},"PeriodicalIF":4.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146077703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}