Current Research in Biotechnology最新文献

{"title":"Differential effect of nine cinnamic acid derivatives on the biocatalytic activity of Candida antarctica lipase type B","authors":"Federico Zappaterra ,&nbsp;Domenico Meola ,&nbsp;Francesco Presini ,&nbsp;Simona Aprile ,&nbsp;Valentina Venturi ,&nbsp;Chiara Nosengo ,&nbsp;Chiara De Luca ,&nbsp;Martina Catani ,&nbsp;Lindomar Alberto Lerin ,&nbsp;Pier Paolo Giovannini","doi":"10.1016/j.crbiot.2024.100231","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100231","url":null,"abstract":"<div><p>Naturally occurring cinnamic acid derivatives are a broad family of aromatic carboxylic acids with bioactive properties. Among the cinnamic acid derivatives, for instance, are ferulic acid and caffeic acid, which have been widely studied for their antioxidant and anti-inflammatory properties. These active ingredients are mostly poorly soluble in water, which greatly limits their bioavailability. To increase the bioavailability of these acids, green esterification protocols can be developed exploiting lipases. In particular, this article reports the process optimization for the enzymatic esterification of nine cinnamic acid derivatives with erythritol, a polyol highly soluble in water. The study explores how the different substituents present on the aromatic ring of the cinnamic acid derivatives affect the catalytic capacity of the <em>Candida antarctica</em> lipase type B. The study, conducted through both molecular docking and experimental evidence, shows how hydroxyl groups on the aromatic ring can strongly limit the conversion of the acids to the corresponding esters. At the same time, the degree of unsaturation of the derivative also influences the favorable poses in the active site of the lipase. The best results (yields over 95 %) were obtained with 10 g/L of lipase, a temperature of 90 °C, molar ratio (acid/alcohol) of 3:1, for 72 h of reaction time.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"8 ","pages":"Article 100231"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000571/pdfft?md5=b874724fa4df134f59458159b3b02bf1&pid=1-s2.0-S2590262824000571-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141434057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peptide lipidation and shortening optimises antibacterial, antibiofilm and membranolytic actions of an amphiphilic polylysine-polyphenyalanine octapeptide","authors":"Bruno Mendes ,&nbsp;Charlotte Edwards-Gayle ,&nbsp;Glyn Barrett","doi":"10.1016/j.crbiot.2024.100240","DOIUrl":"10.1016/j.crbiot.2024.100240","url":null,"abstract":"<div><p>The demand for broad-spectrum antibacterial agents continues with increasing rates of resistance of microbial pathogens to traditional antibiotics. Peptides and lipopeptides are gaining traction as promising novel, class-reference antibiotics for tackling difficult-to-treat infections caused by multi-drug resistant bacteria. To identify novel candidates and expand treatment options in clinical settings, we explored the <em>in vitro</em> antibacterial potential and mode of action of a short octapeptide combining a cationic block of four lysines and a highly hydrophobic segment of four phenylalanines (K4F4), and two K4F4-inspired lipopeptides (Palmitoyl-K4F4 and K4-NH-Palmitoyl). Preliminary AI-based screening had revealed the antimicrobial potential of the K4F4 peptide coupled with limited haemolytic activity. Broth dilution and haemolytic assays have confirmed these <em>in silico</em> predictions. Overall, our lipidated peptides were more active at lower MIC values compared to non-lipidated species, indicating the beneficial impact of tailing lipidation on design of peptide-based antimicrobials. An integrated view of the membrane-active mechanism of these novel therapeutic templates was obtained using a combination of flow cytometry, fluorescence microscopy and dye-based permeabilization assays. K4F4 and its lipidated derivatives act via a fast-disrupting mechanism without inducing bacterial resistance mechanisms in a long-term exposure assay. A K4F4-inspired lipopeptide together with its shorter version (K4-NH-Palmitoyl), were more stable in environments closer emulating physiological conditions, showing a higher antibacterial response in physiological salts and serum than their parent peptide. Our findings reveal the antibacterial and antibiofilm potential of a novel polylysine-polyphenyalanine peptide and highlight the significant contribution of lipidation and shortening as molecular engineering strategies to improve and guide the future design of next-generation membrane-targeting antibiotics.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"8 ","pages":"Article 100240"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000662/pdfft?md5=fddf19f74fab0f84efe1a00be76e50af&pid=1-s2.0-S2590262824000662-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141951456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation of (Aminomethyl)phosphonic acid (AMPA) by isolated microbial consortia extracted from biological filters at drinking water treatment plants","authors":"Laura Pickering ,&nbsp;Miles Folkes ,&nbsp;Barrie Holden ,&nbsp;Peter Jarvis ,&nbsp;Pablo Campo ,&nbsp;Francis Hassard","doi":"10.1016/j.crbiot.2024.100248","DOIUrl":"10.1016/j.crbiot.2024.100248","url":null,"abstract":"<div><p>The widespread use of glyphosate has significantly increased its presence in drinking water sources. Aminomethylphosphonic Acid (AMPA), a breakdown product of glyphosate, is challenging to remove from water using conventional treatment methods, posing risks to public health and environmental safety. This work investigates the biodegradation of AMPA by bacteria isolated from three environmental sources, with a focus on determining their potential application in water treatment systems. Two samples were collected from granular activated carbon (GAC) filters of different operational durations at a water treatment facility, and one sample was taken from soil that had historically been treated with glyphosate-based herbicides. Bacterial isolates capable of degrading AMPA were identified from these samples through selective enrichment, and kinetic degradation experiments were then conducted to assess their effectiveness. In environmental samples, after 48 hrs AMPA removal was &gt; 70 % using GAC from an active treatment plant and soil samples removed 19 %. After bacterial isolation a consortium was isolated and from these four isolates were identified, comprising three species, including novel AMPA degraders M−S3 and M−SS (<em>Myroides sp</em>. mNGS23), and P-S92 (<em>Pseudochrobactrum saccharolyticum</em>). Within both minimal media supplemented with AMPA and raw untreated showing substrate concentrations above 10 mg/L whilst the specific degradation rates saw a decrease in substrate concentrations above 100 mg/L. AMPA removal occured in pilot scale sand filters augmented with P-S92 but removal was inconsistent. These findings show the potential of using biodegradation as an effective treatment strategy for AMPA removal from water. The identification of AMPA-degrading bacteria offers a promising solution for enhancing the removal of this persistent pollutant from contaminated waters. Further research is recommended to explore the full-scale application of these isolates in water treatment processes. This study contributes to the development of sustainable water treatment technologies by harnessing the natural degradative capacities of environmental bacteria.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"8 ","pages":"Article 100248"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000741/pdfft?md5=e374ae94461dd0bd9ef172b781e1577e&pid=1-s2.0-S2590262824000741-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142152050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the gut microbiome: The revolution of artificial intelligence in microbiota analysis and intervention","authors":"Mohammad Abavisani ,&nbsp;Alireza Khoshrou ,&nbsp;Sobhan Karbas Foroushan ,&nbsp;Negar Ebadpour ,&nbsp;Amirhossein Sahebkar","doi":"10.1016/j.crbiot.2024.100211","DOIUrl":"10.1016/j.crbiot.2024.100211","url":null,"abstract":"<div><p>The human gut microbiome is an intricate ecosystem with profound implications for host metabolism, immune function, and neuroendocrine activity. Over the years, studies have strived to decode this microbial universe, especially its interactions with human health and underlying metabolic processes. Traditional analyses often struggle with the complex interplay within the microbiome due to presumptions of microbial independence. In response, machine learning (ML) and deep learning (DL) provide advanced multivariate and non-linear analytical tools that adeptly capture the complex interactions within the microbiota. With the influx of data from metagenomic next-generation sequencing (mNGS), there's an increasing reliance on these artificial intelligence (AI) subsets to derive actionable insights. This review delves deep into the cutting-edge ML techniques tailored for human gut microbiota research. It further underscores the potential of gut microbiota in shaping clinical diagnostics, prognosis, and intervention strategies, pointing to a future where computational methods bridge the gap between microbiome knowledge and targeted health interventions.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100211"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000376/pdfft?md5=245f0081d11c539786fd3fec74e20573&pid=1-s2.0-S2590262824000376-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140795710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of myogenic potential of purebred chicken muscle stem cells","authors":"Hyun Young Jung ,&nbsp;Minsu Kim ,&nbsp;Chang-Kyu Lee ,&nbsp;Myung Yeo ,&nbsp;Sea Hwan Sohn ,&nbsp;Cheorun Jo","doi":"10.1016/j.crbiot.2024.100241","DOIUrl":"10.1016/j.crbiot.2024.100241","url":null,"abstract":"<div><p>We evaluated the myogenic potential of muscle stem cells (MuSCs) derived from three distinct chicken purebreds—Rhode Island Red (RIR), White Leghorn (WL), and Cornish (CN). Chicken MuSCs were isolated from the breast muscles of chicken embryos on embryonic day 18 using a pre-plating method. Subsequently, the cells underwent a three-day proliferation period, followed by a three-day differentiation phase. WL MuSCs exhibited higher myogenic potential initially compared to RIR and CN. Despite a relatively lower proliferation rate, WL demonstrated a higher proportion of PAX7-positive cells and showed higher expression of myogenic regulatory factor genes than the other breeds. During differentiation, MuSCs from WL formed thicker muscle fibers and showed the highest proportion of myosin-heavy chain-positive cells than MuSCs from other breeds. Additionally, the expression of <em>MYH1</em>, associated with muscle maturation, was highest in WL. Overall, this finding suggests that the myogenic potential of MuSCs from WL surpasses those of RIR and CN. Given the fact that WL is primarily used for egg laying with a low growth rate in the traditional poultry industry, the present study highlights the crucial distinction between selecting production characteristics in conventional animal husbandry and those desirable for cultured meat production.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"8 ","pages":"Article 100241"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000674/pdfft?md5=6a87d9b92152f144f5a06ea51c0b0bf6&pid=1-s2.0-S2590262824000674-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141838358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dolastatins and their analogues present a compelling landscape of potential natural and synthetic anticancer drug candidates","authors":"Sudeshna Nandi ,&nbsp;Somanjana Khatua ,&nbsp;Anish Nag ,&nbsp;Surjit Sen ,&nbsp;Nilanjan Chakraborty ,&nbsp;Arghya Naskar ,&nbsp;Krishnendu Acharya ,&nbsp;Reham Hassan Mekky ,&nbsp;María del Mar Contreras ,&nbsp;Daniela Calina ,&nbsp;Irene Dini ,&nbsp;Javad Sharifi-Rad","doi":"10.1016/j.crbiot.2023.100167","DOIUrl":"10.1016/j.crbiot.2023.100167","url":null,"abstract":"<div><p>Human cancer remains a leading cause of global mortality. Traditional treatment methods, while effective are often associated with substantial side effects, high technical requirements, and considerable expenses. Recently, anticancer peptides, such as dolastatin-type peptides naturally found in marine mollusc <em>Dolabella auricularia</em>, have gained attention due to their enhanced characteristics and specific targeting of cancer cells with minimal toxicity to normal cells. This review aims to provide a comprehensive summary of the anticancer activities of natural dolastatins and synthetic analogues over the past 35 years, focusing on their utilization in advancing cancer treatment strategies. This updated review encompasses a detailed analysis of numerous studies demonstrating the cytotoxic effects of dolastatins and their synthetic analogues on various human tumour cell lines. The analysis includes investigations into their ability to activate apoptosis pathways, inhibit cell cycle progression, and indirectly limit inflammation and angiogenesis in tumours. Both natural dolastatins and synthetic analogues have demonstrated significant anticancer properties through a variety of mechanisms <em>in vitro</em> and <em>in vivo</em> pharmacological studies. Some have even advanced to clinical trials, either alone or in combination with other agents, and have shown promising outcomes. The biological activities of dolastatins and their synthetic analogues offer a promising path in the development of more effective and sustainable anticancer drugs. Their specific action on cancer cells and relative non-toxicity to normal cells highlight their potential as superior cancer therapeutic agents. The current study provides a platform for the most recent preclinical and clinical research on dolastatins and their analogues. Further research into these marine peptides may contribute to the development of sustainable and efficient treatment models for cancer, filling a significant gap in the current cancer therapeutic portfolio.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100167"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262823000497/pdfft?md5=671032c9a2d5c7652226befe9bc9afdf&pid=1-s2.0-S2590262823000497-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139019691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drug Discovery, Diagnostic, and therapeutic trends on Mpox: A patent landscape","authors":"Rohit Sharma ,&nbsp;Ruchi Sharma ,&nbsp;Rajeev K. Singla","doi":"10.1016/j.crbiot.2023.100173","DOIUrl":"10.1016/j.crbiot.2023.100173","url":null,"abstract":"<div><p>As of early 2022, Mpox has resurged and expanded globally, posing a new threat to global health. In close to 110 countries, 87,000 confirmed cases and 112 deaths have been reported as on April 18, 2023. Considering the current pandemic crisis and future developments, it is imperative to understand and characterize the global patent scenario of Mpox. In biological sciences, patents are often promising indicators of technological knowledge production. This patent landscape analysis is the first to provide a solid intellectual foundation for the ongoing development of diagnostic measures, therapeutic drug agents, and vaccines for Mpox. Our search encompassed several patent databases to identify patents related to Mpox, including PatSeer, Google patents, <span>lens.org</span><svg><path></path></svg>, Espacenet, and Patentscope. Patenting trends are discussed, and a Whitespace analysis is carried out in this paper, suggesting the knowledge gaps and promising areas for future research on Mpox. This study could serve as an epicentre for provoking ideas and mitigation strategies.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100173"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262823000552/pdfft?md5=77dcdf1dcbd7b09e2657ea1325206c5f&pid=1-s2.0-S2590262823000552-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139021029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Termite management by entomopathogenic fungi: Recent advances and future prospects","authors":"Ali Hassan ,&nbsp;Zhiqiang Li ,&nbsp;Xuguo Zhou ,&nbsp;Jianchu Mo ,&nbsp;Qiuying Huang","doi":"10.1016/j.crbiot.2024.100183","DOIUrl":"10.1016/j.crbiot.2024.100183","url":null,"abstract":"<div><p>Termites are global pest for man-made structures, and in some parts of the world, they infest agricultural crops as well. Termite control relies predominately on chemical insecticides, but the negative impacts of synthetic termiticides on the environment limit their applications. Entomopathogenic fungi (EPF) are considered an environmentally friendly alternative. Nevertheless, termites have developed various defensive behavioral strategies to circumvent EPF. In this review, we summarize (1) the use of EPF as a biological control agent and strategies/defenses of termites that minimize the effectiveness of EPF, (2) the potential of integrating entomopathogenic fungi with RNA interference (RNAi) as an effective termite control strategy, (3) discussed nanoparticles (silver, chitosan, titanium, etc) which are biosynthesized from different EPF. Nanoparticles have the ability to deliver RNAi triggers (dsRNA/siRNA), so we proposed the synthesis of EPF-based nanoparticles and dsRNA/siRNA complex as potential strategy to control termite pests.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100183"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000091/pdfft?md5=32309d3deaa47272e7f9970337110e32&pid=1-s2.0-S2590262824000091-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139633609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developing gold nanoparticle mycelial composites: Effect of nanoparticle surface functionality on Aspergillus niger viability and cell wall biochemistry","authors":"Ayesha Sadaf ,&nbsp;Juwon S. Afolayan ,&nbsp;Carole C. Perry","doi":"10.1016/j.crbiot.2024.100185","DOIUrl":"10.1016/j.crbiot.2024.100185","url":null,"abstract":"<div><p>Functionalized biomaterials are important structures for wide array of applications due to their tunability, low cost of synthesis, and ease of use. The present study explores the quantitative deposition of coated Au nanoparticles (coating agents: cefaclor, glucose, citrate and NaBH₄) on the mycelium of <em>A. niger</em> as well as exploring the consequences of particle adsorption on the biochemistry (MTT assay, melanin, biofilm and glucan levels) of the fungus. Glucose and NaBH₄ coated Au nanoparticles were found to be denser on the mycelium as compared to cefaclor and citrate coated Au nanoparticles as evidenced by SEM-EDX and ICP-MS analysis. Although all fungal samples remained viable after growth in the presence of (10–20 µg/ml) coated gold nanoparticles stress indicators (production of melanin and biofilm formation) varied with the identity of the coated particles. Citrate coated was found to be highest stress inducing with maximum melanin and biofilm levels followed by cefaclor whereas glucose and NaBH₄ had the least effect on the fungus. This study suggests that though the fungus experiences stress under the effect of nanoparticles, it can still survive and grow in their presence to generate a mycelium with the deposited nanoparticles that could be used for various biosensing and environmental applications.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100185"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S259026282400011X/pdfft?md5=000a76f13ce8ccdc926266fba1e12047&pid=1-s2.0-S259026282400011X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139687779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro and in vivo experimental models for cancer immunotherapy study","authors":"Chunye Zhang ,&nbsp;Yuxiang Sui ,&nbsp;Shuai Liu ,&nbsp;Ming Yang","doi":"10.1016/j.crbiot.2024.100210","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100210","url":null,"abstract":"<div><p>Cancer incidence and mortality are increasing globally. Cancer immunotherapies, such as immune checkpoint inhibitors and adoptive cell therapy, have been recognized as a revolutionary treatment approach to combat cancer. However, immunotherapeutic resistance and cancer recurrence after immunotherapy alarm us to further explore the underlying mechanisms and develop new immunotherapies. Experimental models hold great value in cancer research studies such as deciphering the mechanism of tumor initiation and growth, drug discovery, and evaluation of immunotherapy efficacy. The ideal model is expected to recapitulate and mimic the human tumor microenvironment, including biological, physiological, and immunologic functionality. However, each model has its pros and cons, and the selection of a model depends on many factors, such as model features, study aims, and availability of related resources. In this review, we discussed commonly used models currently used in cancer research and immunotherapy, including 2D and 3D in vitro cell culture models such as spheroid, organoid, hydrogel model, and microfluidic chip, and in vivo mouse tumor models such as genetically engineered models, chemically induced models, cell-derived xenograft (CDX) models, patient-derived xenograft (PDX) models, and humanized mouse models. Both in vitro and in vivo preclinical models are powerful tools for studying cancer immunotherapy, but all these models have their limitations. To promote the success of clinical treatment in cancer therapy, advanced model systems that can better recapitulate the human tumor environment and host immune response are preferable options for preclinical study.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100210"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000364/pdfft?md5=03a21e8b61a40cb9300398ce21488acf&pid=1-s2.0-S2590262824000364-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140543165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}