Current Research in Biotechnology最新文献

{"title":"Exploring emerging water treatment technologies for the removal of microbial pathogens","authors":"Oluwatobi Victoria Obayomi ,&nbsp;Damilare Cornelius Olawoyin ,&nbsp;Olumide Oguntimehin ,&nbsp;Lukman Shehu Mustapha ,&nbsp;Samuel Oluwaseun Kolade ,&nbsp;Peter Olusakin Oladoye ,&nbsp;Seungdae Oh ,&nbsp;Kehinde Shola Obayomi","doi":"10.1016/j.crbiot.2024.100252","DOIUrl":"10.1016/j.crbiot.2024.100252","url":null,"abstract":"<div><p>The availability of potable and clean water has become a global challenge. There are many variables that affect how equally people have access to clean water. Disparities are a result of inadequate infrastructure, which includes a deficiency of suitable pipelines, sanitation systems, and water treatment facilities. The presence of pathogenic microbes such as viruses, bacteria and protozoa in water has become a global public health concern. Pathogens present in water caused various disease outbreaks, health emergencies and increased cost of treatments. To address this challenge, a variety of methods for removing microbial pathogens from water sources have been developed and implemented. This review provides a thorough exploration of diverse methods utilized for pathogen removal in water treatment, encompassing physical, chemical, and biological approaches. It delves into the efficacy of each method, scrutinizing their constraints and practical implications. Furthermore, recent advancements and emerging technologies within the domain are explored, offering insights into potential future developments and enhancements. Future research efforts should focus on addressing these challenges to enhance the efficiency, reliability, and sustainability of water treatment systems for safeguarding public health and ensuring access to safe drinking water worldwide.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"8 ","pages":"Article 100252"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000789/pdfft?md5=ab04715655f3b30cc253fb6e727a828c&pid=1-s2.0-S2590262824000789-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142240346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Algal-based membrane bioreactors for effective removal of hazardous and toxic contaminants: A comprehensive review","authors":"Soumitra Nath ,&nbsp;Ashim Das Astapati ,&nbsp;Aniket Naha ,&nbsp;Indu Sharma ,&nbsp;Maulin P. Shah","doi":"10.1016/j.crbiot.2024.100201","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100201","url":null,"abstract":"<div><p>In present times, there is increasing potential of algal-based membrane bioreactors (MBRs) considering the removal of hazardous and toxic contaminants from different wastewater sources. The article summarizes on various types of contaminants that can be effectively removed using algal-based MBRs, including heavy metals and emerging contaminants. The selection criteria, advantages and limitations of different algal species used in algal-based MBRs are also discussed. For optimal performance of algal-based MBRs, operating parameters viz. hydraulic retention time, organic loading rate, nutrient levels, light intensity and duration are essential. The review further highlights the efficient removal of hazardous and toxic contaminants, biomass productivity, and membrane fouling as critical performance indicators. Various applications of algal-based MBRs, particularly in the treatment of municipal and industrial discharges, landfill leachate treatment, and bioremediation of contaminated sites, are also discussed. Finally, the current review identifies the technological limitations, scale-up challenges, and economic feasibility of algal-based MBRs and provides directions of future researches. On the whole, algal-based MBRs offer a sustainable and worthwhile solution in the eradication of harmful and lethal contaminants from various effluent sources.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100201"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000273/pdfft?md5=24ffb892fbacf1e76336638a253a3d23&pid=1-s2.0-S2590262824000273-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140164036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The metatranscriptome of resident microbiota in the gut of blue mussels, Mytilus edulis, under standard laboratory conditions","authors":"Tyler W. Griffin ,&nbsp;Lisa M. Nigro ,&nbsp;Hannah I. Collins ,&nbsp;Bridget A. Holohan ,&nbsp;J. Evan Ward","doi":"10.1016/j.crbiot.2024.100208","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100208","url":null,"abstract":"<div><p>Metatranscriptomic methods involving RNA sequencing (RNA-seq) are powerful tools for evaluating the gene expression profiles of transcriptionally-active microbes that inhabit the tissues of animals. Bivalve molluscs, like all invertebrates, are holobionts and sites of interactions between host animals and both prokaryotic and eukaryotic symbionts. The present communication describes the metatranscriptomic profile of the resident microorganisms that inhabit the gut of blue mussels, <em>Mytilus edulis</em>, under standard laboratory conditions. Each of the eight mussels described herein were housed in isolated microcosms with routine husbandry for 14 days before their gut tissues were sampled and subjected to RNA-seq. Subsequent mRNA reads that aligned to the mussel genome were removed, and the non-host reads were annotated for function and pathway analyses. Under laboratory conditions, the resident gut microbiota expressed genes associated mostly with aerobic energy processes, with other notable contributions from metabolism and protein processing genes. At the pathway level, the most abundant complete pathways expressed in the resident gut microbial communities were related to aerobic cellular respiration, nucleotide biosynthesis, and catabolism. These data represent novel, baseline microbial gene expression information from the gut of mussels, which are crucial for future research examining the mussel holobiont and bivalve microbial ecology. Public retrieval and secondary analyses of these metatranscriptomic profiles are highly encouraged.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100208"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000340/pdfft?md5=01d735a470709f2acfd9618f3fd35fe4&pid=1-s2.0-S2590262824000340-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140351797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A conductive film produced by the supernatant from Serratia marcescens cultivation containing prodigiosin increases electricity generation in a microbial fuel cell","authors":"Ana Clara Bonizol Zani ,&nbsp;João Carlos de Souza ,&nbsp;João Pedro Rueda Furlan ,&nbsp;Eliana Guedes Stehling ,&nbsp;Adalgisa Rodrigues de Andrade ,&nbsp;Valeria Reginatto","doi":"10.1016/j.crbiot.2024.100215","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100215","url":null,"abstract":"<div><p>Although <em>Serratia marcescens</em> is known for its natural ability to produce the red pigment prodigiosin, it has been little explored as a biocatalyst in bioelectrochemical systems (BES). Here, we have employed an environmental <em>S. marcescens</em> isolate S734 as biocatalyst in a microbial fuel cell (MFC) anode to oxidize glycerol and to produce energy; we have evaluated how the anode behaves in three conditions: (i) as an abiotic electrode (FC-A); (ii) as a biotic electrode after <em>S. marcescens</em> biofilm growth (MFC-B); and (iii) as an abiotic electrode added with the supernatant containing prodigiosin (FC-P). Scanning electron microscopy and electrochemical measurements indicated that prodigiosin formed a conductive film over FC-P, which increased charge transfer by 424 times compared to FC-A. The maximum power density during the FC-P operation was 10.0 mW/m⁻². Nevertheless, only in the presence of <em>S. marcescens</em> (MFC-B) was glycerol oxidized and electricity generated. Cyclic voltammetry indicated that the prodigiosin was the electrochemical active substance in the supernatant, and that its process was irreversible and controlled by adsorption. Electrochemical impedance spectroscopy confirmed that the prodigiosin-containing supernatant decreased the load resistance from 8396.3 Ω in FC-A to 58.10 Ω in FC-P. Genomic analysis showed that the prodigiosin biosynthesis gene cluster in strain S734 belonged to the <em>Serratia</em> 274 type, which contains <em>pigA</em> to <em>pigN</em> genes flanked by <em>cueR</em> and <em>copA</em> homologues. In conclusion, the supernatant produced by <em>S. marcescens</em> strain S734, containing prodigiosin could be explored as a green conductor in BES without further purification steps.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100215"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000418/pdfft?md5=582cba5ad88091764dde6770499a9e46&pid=1-s2.0-S2590262824000418-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140644559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expanding the chitin oligosaccharide portfolio by engineering NodC chitin synthases in Escherichia coli","authors":"Chiara Guidi ,&nbsp;Xevi Biarnés ,&nbsp;Antoni Planas ,&nbsp;Marjan De Mey","doi":"10.1016/j.crbiot.2024.100255","DOIUrl":"10.1016/j.crbiot.2024.100255","url":null,"abstract":"<div><p>Synthetic biology greatly accelerated the building process of potential microbial cell factories for the production of industrially relevant compounds, e.g., chitooligosaccharides (COS) which have an enormous application potential in multiple industries, i.e., pharma, cosmetics and agrifood. COS are produced by the heterologous expression of the chitin oligosaccharide synthase, NodC, in <em>Escherichia coli,</em> mainly yielding mixtures of chitintetraose (A4) and/or chitinpentaose (A5). We rationalised here product formation limitations based on molecular modelling of the structures of several NodC enzymes. We used this information to protein engineer NodC, rendering longer COS. Hence, an in vivo platform of defined COS-producing strains with different degrees of polymerisation was developed and experimentally characterised. Significantly, several strains were producing long COS, such as chitinhexaose (A6) and −heptaose (A7), not identified in any other natural producer. Additionally, other engineered strains efficiently produce almost 100% specific A4 or A5 product. Altogether, our results indicate that electrostatics-driven dynamics effects are to be considered in the molecular ruler hypothesis. Charge density at the transmembrane helices of NodC affects the opening of the integral binding pocket and in this way the length of the produced chitin oligomers can be modulated. As a result, the internal ruler mechanism elaborated and validated in this manuscript can serve as a guideline to perform site-directed mutagenesis at positions in related NodC and chitin synthase enzymes for both industrial applications as for identification of therapeutic targets.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"8 ","pages":"Article 100255"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000819/pdfft?md5=bba3a7e17025b0c8fbb38a83b56b1df2&pid=1-s2.0-S2590262824000819-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142240033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering protein translocation pathway to improve recombinant proteins in Pichia pastoris","authors":"Shengyan Wang ,&nbsp;Huijia Dai ,&nbsp;Qingling Tang ,&nbsp;Yujing Yu ,&nbsp;Yaying Xie ,&nbsp;Tao Wang ,&nbsp;Yide Huang","doi":"10.1016/j.crbiot.2024.100182","DOIUrl":"10.1016/j.crbiot.2024.100182","url":null,"abstract":"<div><p><em>Pichia pastoris</em> is one of the most commonly used hosts for producing heterologous proteins, whereas production levels vary depending on the protein of interest and are also regulated by regulatory factors. We conducted RNA-seq by expressing the reporter EGFP and observed significant upregulation of certain subunits (Sec61p, Sbh1p, Sss1p, Sec66p and Sec72p) of the Sec complex in the high-expression recombinant GS115 stains. The overexpression of these genes may increase the expression levels of heterogeneous proteins. In this study, the endogenous promoters of the Sec complex subunits Sbh1p, Sss1p, Sec66p and Sec72p were isolated and verified their activity using the <em>Lac-Z</em> reporter gene. <em>Sss1</em>, <em>Sbh1</em>, <em>Sec66</em> and <em>Sec72</em> were overexpressed under the control of their own promoters in <em>Pichia pastoris</em>, respectively. The overexpression of <em>Sss1</em>, <em>Sbh1</em>, <em>Sec66</em> and <em>Sec72</em> in cells was confirmed by fluorescent microscope and Western blot analysis. The α-amylase was employed to evaluate the effect of overexpression of the Sec subunits on the heterologous protein expression. The results demonstrated that the α-amylase activity increased by 16%, 58%, 16% and 17% in the strains overexpressing <em>Sss1</em>, <em>Sbh1, Sec66</em> and <em>Sec72</em>, respectively. Engineering the protein translocation pathway can be an alternative to enhance heterogeneous proteins in <em>Pichia pastoris</em> expression system.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100182"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S259026282400008X/pdfft?md5=eb38d0464d385675025ebfc06b6a2987&pid=1-s2.0-S259026282400008X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139687650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metagenomics analysis of sediments of river Ganga, India for bacterial diversity, functional genomics, antibiotic resistant genes and virulence factors","authors":"Ajaya Kumar Rout ,&nbsp;Partha Sarathi Tripathy ,&nbsp;Sangita Dixit ,&nbsp;Dibyajyoti Uttameswar Behera ,&nbsp;Bhaskar Behera ,&nbsp;Basanta Kumar Das ,&nbsp;Bijay Kumar Behera","doi":"10.1016/j.crbiot.2024.100187","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100187","url":null,"abstract":"<div><p>The river Ganges in India has faced considerable issues due to water quality degradation caused by various anthropogenic activities. This study employs metagenomic analysis to comprehensively characterize bacterial communities, explore functional genomics, and investigate the prevalence of virulence factors and antibiotic resistant genes (ARGs) within the sediment environment of the river Ganges. Taxonomic profiling revealed that <em>Proteobacteria</em> were the most dominating phyla found in all samples, whereas the abundance of <em>Pseudomonas</em> at the genus level was the highest in all the samples. Functional annotation and pathway analysis uncover the genomic potential of sediment associated bacteria, shedding light on metabolic pathways, biogeochemical processes, human diseases and adaptive mechanisms within the riverine ecosystem. Moreover, identifying the highest number of genes related to virulence factors was observed in K1 samples (3), and the highest number of genes related to ARGs found in K3 (25) samples emphasizes the need to understand potential pathogenicity in these environments. Characterization of ARGs provides crucial insights into the prevalence of resistance determinants, their genetic contexts, and potential sources of antibiotic resistance in this vital aquatic ecosystem. Overall metagenomics analysis in different sampling sites of river Ganga observed nearly the same OTUs at microbial communities at the taxonomic level but not at a functional level. This research can be a critical foundation for assessing the ecological implications of microbial communities, functional genomics, and the resistome in the river Ganges sediments. The findings underscore the importance of metagenomic approaches in elucidating the intricate microbial ecology and the prevalence of genetic elements relevant to environmental health and antimicrobial resistance in aquatic ecosystems. Further investigation would be required to understand the underlying cause behind the restoration of microbial functional profiles, including ARGs and VFs, to unravel the rejuvenation aspects of this unique ecosystem.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100187"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000133/pdfft?md5=e740d72e4853c9d0e2b6beb3b8bacc9b&pid=1-s2.0-S2590262824000133-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139719288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microplastics contamination in food products: Occurrence, analytical techniques and potential impacts on human health","authors":"Suman Giri ,&nbsp;Gopal Lamichhane ,&nbsp;Dipendra Khadka ,&nbsp;Hari Prasad Devkota","doi":"10.1016/j.crbiot.2024.100190","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100190","url":null,"abstract":"<div><p>Chemically, microplastics (MPs) are synthetic materials composed of plastic monomers and additives and vary in size from 0.1-5,000 μm. Due to their chemical stability and the widespread use of plastics for various purposes, MPs pollution of the environment has increased dramatically, leading to the contamination of daily consumer products as well. Although previous studies have reported the environmental impacts of MPs, only a few studies have highlighted the occurrence of MPs in food products and their possible effects on human health. Recent investigations have identified MP particles in drinking water and other beverages, seafood, plant products, salt, sugar, and honey, raising an alarm over the safety and quality of these food items. Ingestion, inhalation, and dermal contact of such food and other consumer goods are the common routes through which MPs may enter the human body and can have several deleterious health impacts including oxidative stress, inflammation, immunotoxicity, increased risk of neoplasia, cellular metabolism impairment, neurotoxicity, gut microbiome dysbiosis, disruption of reproductive system among others. A collective approach employing source control, recycling, biodegradable plastics, strengthening legislation, and bioremediation could be a promising and sustainable solution to control the MPs pollution. The key challenge appears to standarize detection methods along with reducing the MPs contamination from the food products as well as from the environment. Therefore, this review focuses on the occurrence of MPs in several food products, current methods of analysis, potential health impacts, and strategies to mitigate the widespread MPs pollution. It also adds novel findings, knowledge gaps, and recommendations that can guide future research in this field.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100190"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000169/pdfft?md5=d3a8de7d49ca85356a1c5e66a7e45d64&pid=1-s2.0-S2590262824000169-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140024440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of field portable potentiostat using electrochemical aptasensing technology for detection of Cr(VI) in aquatic environment","authors":"Jyotsna Dei ,&nbsp;Soumyadeb Bhattacharyya ,&nbsp;Koustuv Ghosh ,&nbsp;Subrata Sarkar ,&nbsp;Souvik Pal ,&nbsp;Subhankar Mukherjee ,&nbsp;Dhruba Jyoti Sarkar ,&nbsp;Alokesh Ghosh ,&nbsp;Rajib Bandyopadhyay ,&nbsp;Basanta Kumar Das ,&nbsp;Bijay Kumar Behera","doi":"10.1016/j.crbiot.2024.100193","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100193","url":null,"abstract":"<div><p>Hexavalent chromium or Cr(VI) is highly toxic for humans as it causes high oxidative reactions inside cells, leading to diseases like chronic ulcers and damage to the kidneys, mucous membranes, throat, skin, and respiratory tract. Due to rapid urbanization, Cr(VI) comes into our food chain through unmonitored and uncontrolled application in agriculture fields, refineries, mills, the tanning industry, automobiles, road works, etc. Presently, the standard Cr(VI) detection is done using conventional processes, which, though accurate, has severe drawbacks in on-the-spot rapid detection in the field. Here, we have represented a handheld potentiostat towards trace-level Cr(VI) detection in aquatic environments. A Cr(VI) specific DNA aptamer immobilized screen printed electrode (SPE) has been used as the main biosensor. The device operates on an electronic peak current dumping event through mass deposition in the presence of an aptamer coupled with Cr(VI) onto the working electrode. The working range of the developed prototype is in the range of 0–1000 ppb Cr(VI), where the maximum linearity has been observed in the range of 0–500 ppb with a limit of Detection (LOD) as low as 10 ppb. The device has exhibited an excellent correlation with commercially available electrochemical workstations with a coefficient of 0.972. Moreover, the applicability of the developed device has been validated for 7 different types of water samples. To our knowledge, this is the first-ever reported simplistic resource-limited on-spot aptasensing device for Cr(VI) detection in an aquatic environment.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100193"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000194/pdfft?md5=fe1a4ec25f5718704121e5b717e7fe7c&pid=1-s2.0-S2590262824000194-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140042236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition mechanisms of high salinities on flocculation of marine Algal-Mycelial pellets","authors":"Sujie Li ,&nbsp;Shuyi Xie ,&nbsp;Yuting Yang ,&nbsp;Xunan Yang ,&nbsp;Yaobin Lu ,&nbsp;Lijuan Luo ,&nbsp;Shanshan Chen ,&nbsp;Tiangang Luan","doi":"10.1016/j.crbiot.2024.100222","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100222","url":null,"abstract":"<div><p>Microalgae are widely used in wastewater treatment because they can absorb nitrogen and phosphorus pollutions and reduce CO₂ emissions. However, they are hard to collected due to tiny cell sizes. Flocculation of microalgae by fungi to form the algal-mycelial pellets (AMPs) is one of the efficient collecting methods from wastewater. With the large amount of saline wastewater being discharged, the flocculating effects and mechanisms of AMPs in high saline wastewater are still unknown. Flocculation experiments were performed by <em>Aspergillus niger</em> and <em>Chlorella</em> sp. to study the effects and mechanisms in 0 %-4% salinities. Results showed that the flocculating efficiency (FE) in the 0 %–2% salinities exceeded 95 % at 24 h, whereas the FE reached only 63 % ± 2 % in the 4 % salinity. The flocculating biomass were also decreased with the increasing salinity. Fungi pellets increased in volume and mass at high salinity, resulting in a more compact mycelium structure with less space for microalgae to attach, which was not conducive to flocculation. Furthermore, contents of proteins (PN) and polysaccharides (PS) in the tightly bound EPS (TB-EPS) of AMPs at 4 % salinity decreased by 44 % ± 8 % and 33 % ± 4 % respectively compared to those at 0 % salinity. The decrease in the content of PN and PS led to a weakening of hydrophobicity, a rise in electrostatic repulsion, and an increase in the energy barrier of AMPs, all of which impeded flocculation. This study will provide theoretical bases for the treatment and the recovery of microalgae in high saline wastewater.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100222"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000480/pdfft?md5=04ece61ce377c843e6b54da6b58a8c7c&pid=1-s2.0-S2590262824000480-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140816925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}