Current Research in Biotechnology最新文献

{"title":"Deciphering the gut microbiome: The revolution of artificial intelligence in microbiota analysis and intervention","authors":"Mohammad Abavisani ,&nbsp;Alireza Khoshrou ,&nbsp;Sobhan Karbas Foroushan ,&nbsp;Negar Ebadpour ,&nbsp;Amirhossein Sahebkar","doi":"10.1016/j.crbiot.2024.100211","DOIUrl":"10.1016/j.crbiot.2024.100211","url":null,"abstract":"<div><p>The human gut microbiome is an intricate ecosystem with profound implications for host metabolism, immune function, and neuroendocrine activity. Over the years, studies have strived to decode this microbial universe, especially its interactions with human health and underlying metabolic processes. Traditional analyses often struggle with the complex interplay within the microbiome due to presumptions of microbial independence. In response, machine learning (ML) and deep learning (DL) provide advanced multivariate and non-linear analytical tools that adeptly capture the complex interactions within the microbiota. With the influx of data from metagenomic next-generation sequencing (mNGS), there's an increasing reliance on these artificial intelligence (AI) subsets to derive actionable insights. This review delves deep into the cutting-edge ML techniques tailored for human gut microbiota research. It further underscores the potential of gut microbiota in shaping clinical diagnostics, prognosis, and intervention strategies, pointing to a future where computational methods bridge the gap between microbiome knowledge and targeted health interventions.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100211"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000376/pdfft?md5=245f0081d11c539786fd3fec74e20573&pid=1-s2.0-S2590262824000376-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140795710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of myogenic potential of purebred chicken muscle stem cells","authors":"Hyun Young Jung ,&nbsp;Minsu Kim ,&nbsp;Chang-Kyu Lee ,&nbsp;Myung Yeo ,&nbsp;Sea Hwan Sohn ,&nbsp;Cheorun Jo","doi":"10.1016/j.crbiot.2024.100241","DOIUrl":"10.1016/j.crbiot.2024.100241","url":null,"abstract":"<div><p>We evaluated the myogenic potential of muscle stem cells (MuSCs) derived from three distinct chicken purebreds—Rhode Island Red (RIR), White Leghorn (WL), and Cornish (CN). Chicken MuSCs were isolated from the breast muscles of chicken embryos on embryonic day 18 using a pre-plating method. Subsequently, the cells underwent a three-day proliferation period, followed by a three-day differentiation phase. WL MuSCs exhibited higher myogenic potential initially compared to RIR and CN. Despite a relatively lower proliferation rate, WL demonstrated a higher proportion of PAX7-positive cells and showed higher expression of myogenic regulatory factor genes than the other breeds. During differentiation, MuSCs from WL formed thicker muscle fibers and showed the highest proportion of myosin-heavy chain-positive cells than MuSCs from other breeds. Additionally, the expression of <em>MYH1</em>, associated with muscle maturation, was highest in WL. Overall, this finding suggests that the myogenic potential of MuSCs from WL surpasses those of RIR and CN. Given the fact that WL is primarily used for egg laying with a low growth rate in the traditional poultry industry, the present study highlights the crucial distinction between selecting production characteristics in conventional animal husbandry and those desirable for cultured meat production.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"8 ","pages":"Article 100241"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000674/pdfft?md5=6a87d9b92152f144f5a06ea51c0b0bf6&pid=1-s2.0-S2590262824000674-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141838358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dolastatins and their analogues present a compelling landscape of potential natural and synthetic anticancer drug candidates","authors":"Sudeshna Nandi ,&nbsp;Somanjana Khatua ,&nbsp;Anish Nag ,&nbsp;Surjit Sen ,&nbsp;Nilanjan Chakraborty ,&nbsp;Arghya Naskar ,&nbsp;Krishnendu Acharya ,&nbsp;Reham Hassan Mekky ,&nbsp;María del Mar Contreras ,&nbsp;Daniela Calina ,&nbsp;Irene Dini ,&nbsp;Javad Sharifi-Rad","doi":"10.1016/j.crbiot.2023.100167","DOIUrl":"10.1016/j.crbiot.2023.100167","url":null,"abstract":"<div><p>Human cancer remains a leading cause of global mortality. Traditional treatment methods, while effective are often associated with substantial side effects, high technical requirements, and considerable expenses. Recently, anticancer peptides, such as dolastatin-type peptides naturally found in marine mollusc <em>Dolabella auricularia</em>, have gained attention due to their enhanced characteristics and specific targeting of cancer cells with minimal toxicity to normal cells. This review aims to provide a comprehensive summary of the anticancer activities of natural dolastatins and synthetic analogues over the past 35 years, focusing on their utilization in advancing cancer treatment strategies. This updated review encompasses a detailed analysis of numerous studies demonstrating the cytotoxic effects of dolastatins and their synthetic analogues on various human tumour cell lines. The analysis includes investigations into their ability to activate apoptosis pathways, inhibit cell cycle progression, and indirectly limit inflammation and angiogenesis in tumours. Both natural dolastatins and synthetic analogues have demonstrated significant anticancer properties through a variety of mechanisms <em>in vitro</em> and <em>in vivo</em> pharmacological studies. Some have even advanced to clinical trials, either alone or in combination with other agents, and have shown promising outcomes. The biological activities of dolastatins and their synthetic analogues offer a promising path in the development of more effective and sustainable anticancer drugs. Their specific action on cancer cells and relative non-toxicity to normal cells highlight their potential as superior cancer therapeutic agents. The current study provides a platform for the most recent preclinical and clinical research on dolastatins and their analogues. Further research into these marine peptides may contribute to the development of sustainable and efficient treatment models for cancer, filling a significant gap in the current cancer therapeutic portfolio.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100167"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262823000497/pdfft?md5=671032c9a2d5c7652226befe9bc9afdf&pid=1-s2.0-S2590262823000497-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139019691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drug Discovery, Diagnostic, and therapeutic trends on Mpox: A patent landscape","authors":"Rohit Sharma ,&nbsp;Ruchi Sharma ,&nbsp;Rajeev K. Singla","doi":"10.1016/j.crbiot.2023.100173","DOIUrl":"10.1016/j.crbiot.2023.100173","url":null,"abstract":"<div><p>As of early 2022, Mpox has resurged and expanded globally, posing a new threat to global health. In close to 110 countries, 87,000 confirmed cases and 112 deaths have been reported as on April 18, 2023. Considering the current pandemic crisis and future developments, it is imperative to understand and characterize the global patent scenario of Mpox. In biological sciences, patents are often promising indicators of technological knowledge production. This patent landscape analysis is the first to provide a solid intellectual foundation for the ongoing development of diagnostic measures, therapeutic drug agents, and vaccines for Mpox. Our search encompassed several patent databases to identify patents related to Mpox, including PatSeer, Google patents, <span>lens.org</span><svg><path></path></svg>, Espacenet, and Patentscope. Patenting trends are discussed, and a Whitespace analysis is carried out in this paper, suggesting the knowledge gaps and promising areas for future research on Mpox. This study could serve as an epicentre for provoking ideas and mitigation strategies.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100173"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262823000552/pdfft?md5=77dcdf1dcbd7b09e2657ea1325206c5f&pid=1-s2.0-S2590262823000552-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139021029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Termite management by entomopathogenic fungi: Recent advances and future prospects","authors":"Ali Hassan ,&nbsp;Zhiqiang Li ,&nbsp;Xuguo Zhou ,&nbsp;Jianchu Mo ,&nbsp;Qiuying Huang","doi":"10.1016/j.crbiot.2024.100183","DOIUrl":"10.1016/j.crbiot.2024.100183","url":null,"abstract":"<div><p>Termites are global pest for man-made structures, and in some parts of the world, they infest agricultural crops as well. Termite control relies predominately on chemical insecticides, but the negative impacts of synthetic termiticides on the environment limit their applications. Entomopathogenic fungi (EPF) are considered an environmentally friendly alternative. Nevertheless, termites have developed various defensive behavioral strategies to circumvent EPF. In this review, we summarize (1) the use of EPF as a biological control agent and strategies/defenses of termites that minimize the effectiveness of EPF, (2) the potential of integrating entomopathogenic fungi with RNA interference (RNAi) as an effective termite control strategy, (3) discussed nanoparticles (silver, chitosan, titanium, etc) which are biosynthesized from different EPF. Nanoparticles have the ability to deliver RNAi triggers (dsRNA/siRNA), so we proposed the synthesis of EPF-based nanoparticles and dsRNA/siRNA complex as potential strategy to control termite pests.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100183"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000091/pdfft?md5=32309d3deaa47272e7f9970337110e32&pid=1-s2.0-S2590262824000091-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139633609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developing gold nanoparticle mycelial composites: Effect of nanoparticle surface functionality on Aspergillus niger viability and cell wall biochemistry","authors":"Ayesha Sadaf ,&nbsp;Juwon S. Afolayan ,&nbsp;Carole C. Perry","doi":"10.1016/j.crbiot.2024.100185","DOIUrl":"10.1016/j.crbiot.2024.100185","url":null,"abstract":"<div><p>Functionalized biomaterials are important structures for wide array of applications due to their tunability, low cost of synthesis, and ease of use. The present study explores the quantitative deposition of coated Au nanoparticles (coating agents: cefaclor, glucose, citrate and NaBH₄) on the mycelium of <em>A. niger</em> as well as exploring the consequences of particle adsorption on the biochemistry (MTT assay, melanin, biofilm and glucan levels) of the fungus. Glucose and NaBH₄ coated Au nanoparticles were found to be denser on the mycelium as compared to cefaclor and citrate coated Au nanoparticles as evidenced by SEM-EDX and ICP-MS analysis. Although all fungal samples remained viable after growth in the presence of (10–20 µg/ml) coated gold nanoparticles stress indicators (production of melanin and biofilm formation) varied with the identity of the coated particles. Citrate coated was found to be highest stress inducing with maximum melanin and biofilm levels followed by cefaclor whereas glucose and NaBH₄ had the least effect on the fungus. This study suggests that though the fungus experiences stress under the effect of nanoparticles, it can still survive and grow in their presence to generate a mycelium with the deposited nanoparticles that could be used for various biosensing and environmental applications.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100185"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S259026282400011X/pdfft?md5=000a76f13ce8ccdc926266fba1e12047&pid=1-s2.0-S259026282400011X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139687779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro and in vivo experimental models for cancer immunotherapy study","authors":"Chunye Zhang ,&nbsp;Yuxiang Sui ,&nbsp;Shuai Liu ,&nbsp;Ming Yang","doi":"10.1016/j.crbiot.2024.100210","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100210","url":null,"abstract":"<div><p>Cancer incidence and mortality are increasing globally. Cancer immunotherapies, such as immune checkpoint inhibitors and adoptive cell therapy, have been recognized as a revolutionary treatment approach to combat cancer. However, immunotherapeutic resistance and cancer recurrence after immunotherapy alarm us to further explore the underlying mechanisms and develop new immunotherapies. Experimental models hold great value in cancer research studies such as deciphering the mechanism of tumor initiation and growth, drug discovery, and evaluation of immunotherapy efficacy. The ideal model is expected to recapitulate and mimic the human tumor microenvironment, including biological, physiological, and immunologic functionality. However, each model has its pros and cons, and the selection of a model depends on many factors, such as model features, study aims, and availability of related resources. In this review, we discussed commonly used models currently used in cancer research and immunotherapy, including 2D and 3D in vitro cell culture models such as spheroid, organoid, hydrogel model, and microfluidic chip, and in vivo mouse tumor models such as genetically engineered models, chemically induced models, cell-derived xenograft (CDX) models, patient-derived xenograft (PDX) models, and humanized mouse models. Both in vitro and in vivo preclinical models are powerful tools for studying cancer immunotherapy, but all these models have their limitations. To promote the success of clinical treatment in cancer therapy, advanced model systems that can better recapitulate the human tumor environment and host immune response are preferable options for preclinical study.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100210"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000364/pdfft?md5=03a21e8b61a40cb9300398ce21488acf&pid=1-s2.0-S2590262824000364-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140543165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring emerging water treatment technologies for the removal of microbial pathogens","authors":"Oluwatobi Victoria Obayomi ,&nbsp;Damilare Cornelius Olawoyin ,&nbsp;Olumide Oguntimehin ,&nbsp;Lukman Shehu Mustapha ,&nbsp;Samuel Oluwaseun Kolade ,&nbsp;Peter Olusakin Oladoye ,&nbsp;Seungdae Oh ,&nbsp;Kehinde Shola Obayomi","doi":"10.1016/j.crbiot.2024.100252","DOIUrl":"10.1016/j.crbiot.2024.100252","url":null,"abstract":"<div><p>The availability of potable and clean water has become a global challenge. There are many variables that affect how equally people have access to clean water. Disparities are a result of inadequate infrastructure, which includes a deficiency of suitable pipelines, sanitation systems, and water treatment facilities. The presence of pathogenic microbes such as viruses, bacteria and protozoa in water has become a global public health concern. Pathogens present in water caused various disease outbreaks, health emergencies and increased cost of treatments. To address this challenge, a variety of methods for removing microbial pathogens from water sources have been developed and implemented. This review provides a thorough exploration of diverse methods utilized for pathogen removal in water treatment, encompassing physical, chemical, and biological approaches. It delves into the efficacy of each method, scrutinizing their constraints and practical implications. Furthermore, recent advancements and emerging technologies within the domain are explored, offering insights into potential future developments and enhancements. Future research efforts should focus on addressing these challenges to enhance the efficiency, reliability, and sustainability of water treatment systems for safeguarding public health and ensuring access to safe drinking water worldwide.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"8 ","pages":"Article 100252"},"PeriodicalIF":3.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000789/pdfft?md5=ab04715655f3b30cc253fb6e727a828c&pid=1-s2.0-S2590262824000789-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142240346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent technologies for transforming textile waste into value-added products: A review","authors":"Manikant Tripathi ,&nbsp;Minaxi Sharma ,&nbsp;Saroj Bala ,&nbsp;Vijay Kumar Thakur ,&nbsp;Anoop Singh ,&nbsp;Kavya Dashora ,&nbsp;Phil Hart ,&nbsp;Vijai Kumar Gupta","doi":"10.1016/j.crbiot.2024.100225","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100225","url":null,"abstract":"<div><p>The disposal of textile waste has become a growing issue worldwide. The rising consumption of clothing and textile materials has resulted in high waste generation. This could adversely impact environmental health, including humans, animals and plants. The three textile recycling methods can be divided into mechanical, chemical, and biological processes. There has been a focus on mechanical and chemical processes, pyrolysis, enzymatic hydrolysis, biological recycling, and microbial engineering in the area of textile waste managment. This review highlight the important parameters that affect the textile recycling performance and are significant for the success of the transformation process. To reach the zero-waste goal, textile recycling and converting this waste into value-added bioproducts are the necessary steps. The present review addresses the current status of textile recycling strategies, and the valorisation processes for converting textile waste materials into value-added products like biofuels, bioplastics, and others sustainable materials.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100225"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000510/pdfft?md5=42ac1e9f7b5a630cf5089c05b42ddddd&pid=1-s2.0-S2590262824000510-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140905651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition mechanisms of high salinities on flocculation of marine Algal-Mycelial pellets","authors":"Sujie Li ,&nbsp;Shuyi Xie ,&nbsp;Yuting Yang ,&nbsp;Xunan Yang ,&nbsp;Yaobin Lu ,&nbsp;Lijuan Luo ,&nbsp;Shanshan Chen ,&nbsp;Tiangang Luan","doi":"10.1016/j.crbiot.2024.100222","DOIUrl":"https://doi.org/10.1016/j.crbiot.2024.100222","url":null,"abstract":"<div><p>Microalgae are widely used in wastewater treatment because they can absorb nitrogen and phosphorus pollutions and reduce CO₂ emissions. However, they are hard to collected due to tiny cell sizes. Flocculation of microalgae by fungi to form the algal-mycelial pellets (AMPs) is one of the efficient collecting methods from wastewater. With the large amount of saline wastewater being discharged, the flocculating effects and mechanisms of AMPs in high saline wastewater are still unknown. Flocculation experiments were performed by <em>Aspergillus niger</em> and <em>Chlorella</em> sp. to study the effects and mechanisms in 0 %-4% salinities. Results showed that the flocculating efficiency (FE) in the 0 %–2% salinities exceeded 95 % at 24 h, whereas the FE reached only 63 % ± 2 % in the 4 % salinity. The flocculating biomass were also decreased with the increasing salinity. Fungi pellets increased in volume and mass at high salinity, resulting in a more compact mycelium structure with less space for microalgae to attach, which was not conducive to flocculation. Furthermore, contents of proteins (PN) and polysaccharides (PS) in the tightly bound EPS (TB-EPS) of AMPs at 4 % salinity decreased by 44 % ± 8 % and 33 % ± 4 % respectively compared to those at 0 % salinity. The decrease in the content of PN and PS led to a weakening of hydrophobicity, a rise in electrostatic repulsion, and an increase in the energy barrier of AMPs, all of which impeded flocculation. This study will provide theoretical bases for the treatment and the recovery of microalgae in high saline wastewater.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100222"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262824000480/pdfft?md5=04ece61ce377c843e6b54da6b58a8c7c&pid=1-s2.0-S2590262824000480-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140816925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}