Development of field portable potentiostat using electrochemical aptasensing technology for detection of Cr(VI) in aquatic environment

Abstract

Hexavalent chromium or Cr(VI) is highly toxic for humans as it causes high oxidative reactions inside cells, leading to diseases like chronic ulcers and damage to the kidneys, mucous membranes, throat, skin, and respiratory tract. Due to rapid urbanization, Cr(VI) comes into our food chain through unmonitored and uncontrolled application in agriculture fields, refineries, mills, the tanning industry, automobiles, road works, etc. Presently, the standard Cr(VI) detection is done using conventional processes, which, though accurate, has severe drawbacks in on-the-spot rapid detection in the field. Here, we have represented a handheld potentiostat towards trace-level Cr(VI) detection in aquatic environments. A Cr(VI) specific DNA aptamer immobilized screen printed electrode (SPE) has been used as the main biosensor. The device operates on an electronic peak current dumping event through mass deposition in the presence of an aptamer coupled with Cr(VI) onto the working electrode. The working range of the developed prototype is in the range of 0–1000 ppb Cr(VI), where the maximum linearity has been observed in the range of 0–500 ppb with a limit of Detection (LOD) as low as 10 ppb. The device has exhibited an excellent correlation with commercially available electrochemical workstations with a coefficient of 0.972. Moreover, the applicability of the developed device has been validated for 7 different types of water samples. To our knowledge, this is the first-ever reported simplistic resource-limited on-spot aptasensing device for Cr(VI) detection in an aquatic environment.