Journal of Veterinary and Animal Sciences最新文献_第5页

Expression of Angiopoietin 2 in ovarian follicles of crossbred Malabari goat 杂交马拉巴里山羊卵巢卵泡血管生成素2的表达

Journal of Veterinary and Animal Sciences Pub Date : 2023-01-01 DOI: 10.51966/jvas.2023.54.1.43-52

M.S. Adithian, V. Babitha, R. Uma, I. Sajitha, E. Navya

{"title":"Expression of Angiopoietin 2 in ovarian follicles of crossbred Malabari goat","authors":"M.S. Adithian, V. Babitha, R. Uma, I. Sajitha, E. Navya","doi":"10.51966/jvas.2023.54.1.43-52","DOIUrl":"https://doi.org/10.51966/jvas.2023.54.1.43-52","url":null,"abstract":"Angiopoietin-2 (Ang-2) is a local ovarian growth factor belonging to the angiopoietin/Tie (tyrosine kinase with Ig and EGF homology domains) signalling pathway, one of the main pathways involved in ovarian follicular angiogenesis. The main objective of this study is to assess the differential protein expression of Ang-2 in the developmental phases of ovarian follicles of crossbred Malabari goats employing an immunohistochemical technique. Ang-2 protein expression was found in all developmental phases of ovarian follicles, including primordial, primary, small, and large antral ovarian follicles. Ang-2 was found in the granulosa cells, theca interna cells, follicular fluid (granulosa cells in the FF), endothelial cells of capillaries and germinal epithelium of the goat ovaries. The Quantitative Method-Percentage Positivity of Ang-2 expression revealed that it was higher in the initial stages of ovarian follicular development and decreased with later stages of follicular development in cross-bred Malabari goats. Moreover, the protein expression of Ang2 was found to be higher in granulosa cells (GC) than in theca interna (TI) cells of antral follicles as evident from the higher staining intensity in GC cells compared to TI cells. It is therefore concluded that the Ang-2 protein expression in ovarian follicular cells decreased with an increase in follicle size and development and based on the combined scores for immunostaining (staining intensity and the number of positive staining cells), the Ang-2 was found to be expressed more in the granulosa cell layer compared to the theca interna cells of the antral follicle in crossbred Malabari goats.","PeriodicalId":52568,"journal":{"name":"Journal of Veterinary and Animal Sciences","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70628450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of serum mineral status in dermatological disorders of dogs 犬皮肤病患者血清矿物质状态的评价

Journal of Veterinary and Animal Sciences Pub Date : 2023-01-01 DOI: 10.51966/jvas.2023.54.2.354-359

G. Sai, V. Babitha, V. Ramnath, V. Beena, N. Unny

{"title":"Evaluation of serum mineral status in dermatological disorders of dogs","authors":"G. Sai, V. Babitha, V. Ramnath, V. Beena, N. Unny","doi":"10.51966/jvas.2023.54.2.354-359","DOIUrl":"https://doi.org/10.51966/jvas.2023.54.2.354-359","url":null,"abstract":"The study was undertaken to evaluate serum mineral status, specifically zinc, copper and selenium in dogs affected with dermatological disorders in comparison to normal healthy dogs. The study included 40 dogs of 2-5 years of age with skin diseases presented to University Veterinary Hospitals at Mannuthy and Kokkalai under the Kerala Veterinary and Animal Sciences University, and ten apparently healthy dogs brought to the hospital for routine health check-up which served as control. Serum concentrations of the three minerals were compared not only between normal and dogs with skin diseases as a whole but also between individual skin diseases- six different skin diseases- with the control group. On comparing the dogs with skin ailments (n=40) and control group, the serum mineral levels analysed through Atomic Absorption Spectrophotometry (AAS) revealed that the concentration of zinc was significantly lower (p<0.01) in the diseased group (0.53 ± 0.04 mg/L) of dogs (n=40) than the control group (1.11 ± 0.09 mg/L) and the serum copper concentration was also significantly reduced (p<0.05) in diseased dogs (0.91 ± 0.08 mg/L) with respect to the control animals (1.23 ± 0.11 mg/L). The serum selenium concentration of diseased group of animals(n=40), however failed to show any significant difference from the control group of animals. The outcome of the study suggests a need for supplementation of zinc and copper in dogs with skin ailments like canine demodicosis that relapse even after prolonged therapy with acaricides.","PeriodicalId":52568,"journal":{"name":"Journal of Veterinary and Animal Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70629012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary inclusion of flaxseed oil, fish oil and their combinations on production performance and egg quality traits in White Leghorn hens 饲粮中添加亚麻籽油、鱼油及其组合对来港白母鸡生产性能和蛋品质性状的影响

Journal of Veterinary and Animal Sciences Pub Date : 2023-01-01 DOI: 10.51966/jvas.2023.54.2.360-367

S. Archana, S. Sankaralingam, P. Anitha, B. Joseph, V. Vasudevan

引用次数: 0

Effect of ginger extract on sensory quality of spent Japanese quail meat tandoori 姜提取物对废鹌鹑肉感官品质的影响

Journal of Veterinary and Animal Sciences Pub Date : 2023-01-01 DOI: 10.51966/jvas.2023.54.1.98-104

D. Santhi, A. Kalaikannan, K. Rajendran

{"title":"Effect of ginger extract on sensory quality of spent Japanese quail meat tandoori","authors":"D. Santhi, A. Kalaikannan, K. Rajendran","doi":"10.51966/jvas.2023.54.1.98-104","DOIUrl":"https://doi.org/10.51966/jvas.2023.54.1.98-104","url":null,"abstract":"Spent Japanese quail (SJQ) meat was tenderized with ginger extract and utilized in the preparation of tandoori. The ginger extract level for tenderization of SJQ meat was optimized by physico-chemical and sensory analyses. The SJQ meat was treated with two levels of ginger extract and used for tandoori preparation along with two controls (broiler quail meat and spent quail meat). Japanese quail tandoori was prepared as four treatments namely CYB (Control young broiler), CSA (Control spent adult), G20 (Spent adult - ginger treatment 20%) and G40 (Spent adult - ginger treatment 40%). The aqueous ginger extract was prepared from fresh ginger rhizome (Zingiber officinale Roscoe). The SJQ was marinated in ginger extract marinade, tumbled, and placed in the chiller (4±1⁰C) for 24 hours and utilized for tandoori preparation. The cooked tandoori of all the treatments were analysed for the physico-chemical and sensory parameters. With ginger extract marination, the pH of cooked tandoori was not affected and the product yield was improved. In the organoleptic evaluation, the appearance score increased in the treatment with 20% ginger extract. The flavour score was significantly (p<0.05) higher in CYB and G20. The SJQ was significantly (p<0.05) tenderized by treatment with 20% and 40% ginger extract. CYB and G20 had significantly (p<0.01) higher scores for overall acceptability scores. It is concluded that 20% ginger extract level could effectively be used to tenderize the SJQ meat for the preparation of tandoori without affecting the physico-chemical and sensory properties.","PeriodicalId":52568,"journal":{"name":"Journal of Veterinary and Animal Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70629268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Observations on tiletamine-zolazepambutorphanol- dexmedetomidine anaesthesia in six dogs undergoing various surgeries 替letamine-zolazepambutorphanol-右美托咪定麻醉6只手术犬的观察

Journal of Veterinary and Animal Sciences Pub Date : 2023-01-01 DOI: 10.51966/jvas.2023.54.2.336-342

Sruthi Chandramohan, S. Sooryadas, P. Dinesh, N. S. Jineshkumar, V. Remya, S. Anoop, P. David

引用次数: 0

Determination of the median lethal dose of indoxacarb pesticide in Wistar rat 茚虫威农药对Wistar大鼠中位致死量的测定

Journal of Veterinary and Animal Sciences Pub Date : 2023-01-01 DOI: 10.51966/jvas.2023.54.2.510-515

A. Raj, N. Nair, A. Nisha, S. Sujith, C. Divya, R. Shankar

引用次数: 0

Analysis of differential expression of microRNA, bta-miR-93 in lipopolysaccharide challenged peripheral blood mononuclear cells of cattle 脂多糖刺激牛外周血单个核细胞中microRNA、bta-miR-93的差异表达分析

Journal of Veterinary and Animal Sciences Pub Date : 2023-01-01 DOI: 10.51966/jvas.2023.54.2.457-464

A. Venu, P. Divya, R. Uma, V. P. Panicker, V. Ramnath, M. Shynu., M. Chinnu

{"title":"Analysis of differential expression of microRNA, bta-miR-93 in lipopolysaccharide challenged peripheral blood mononuclear cells of cattle","authors":"A. Venu, P. Divya, R. Uma, V. P. Panicker, V. Ramnath, M. Shynu., M. Chinnu","doi":"10.51966/jvas.2023.54.2.457-464","DOIUrl":"https://doi.org/10.51966/jvas.2023.54.2.457-464","url":null,"abstract":"The present study was undertaken to analyse the differences in the microRNA (miRNA) expression in lipopolysaccharide (LPS) challenged and unchallenged peripheral blood mononuclear cells (PBMCs) of cattle. The research work was carried out in adult apparently healthy female crossbred cattle maintained at the University Livestock Farm and Fodder Research and Development Scheme, Mannuthy. The expression of miRNA and bta-miR-93 was profiled in the present study by qRT-PCR assay. In silico analysis was done using various online bioinformatic tools for the prediction of target genes, gene ontology analysis and also to study the cellular pathways associated to the target genes of bta-miR-93. The expression analysis of bta-miR- 93 showed decrease in its expression in LPS treated PBMCs when compared to control cells. Besides, significant enrichment of target genes of bta-miR-93 was noticed in many immune-related GO terms and in critical immune-associated cellular pathways. The findings of the current study will help in understanding the regulatory role of bta-miR-93 in LPS-mediated immune responses in cattle.","PeriodicalId":52568,"journal":{"name":"Journal of Veterinary and Animal Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70629745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of factors associated with biosecurity, antimicrobial use and mortality in broiler farms in Northern Kerala 喀拉拉邦北部肉鸡养殖场生物安全、抗菌药物使用和死亡率相关因素评价

Journal of Veterinary and Animal Sciences Pub Date : 2023-01-01 DOI: 10.51966/jvas.2023.54.2.583-588

S. Femin, R. Akash, C. Sachin, S. Sumayya, S. Aarathi, P. Deepa

{"title":"Evaluation of factors associated with biosecurity, antimicrobial use and mortality in broiler farms in Northern Kerala","authors":"S. Femin, R. Akash, C. Sachin, S. Sumayya, S. Aarathi, P. Deepa","doi":"10.51966/jvas.2023.54.2.583-588","DOIUrl":"https://doi.org/10.51966/jvas.2023.54.2.583-588","url":null,"abstract":"A survey was conducted (March 2020 to October 2020) on biosecurity practices, disease occurrence and antimicrobial usage of 77 broiler farms from five districts of Northern Kerala. Data were collected through a questionnaire and field-level disease diagnostic tests were conducted. Biosecurity status varied from farm to farm with 97.4 per cent promoted vaccination and 61.04 per cent disposed carcasses by burning. However, limitations were observed in the use of a footbath, quarantine of new stock, isolation of diseased birds and quality of water. The five most common diseases recorded were Chronic respiratory disease (48.1%), Acute Death Syndrome (27.3%), Colibacillosis (23.4%), Pullorum (18.2%) and Coccidiosis (18.2%). The commonly used antibiotics in poultry farms were Enrofloxacin (35.1%), Cephalexin (14.3%), Tylosin (11.7%) and Levofloxacin (10.4%). Some of the farmers (14.3%) were unaware of the antibiotics used in their farms, a potent risk factor for the emergence of antimicrobial resistance. About 19.49 per cent of farms restricted antibiotic use and 44.15 per cent of farms supported herbal traditional medicines as an alternative to antimicrobials.","PeriodicalId":52568,"journal":{"name":"Journal of Veterinary and Animal Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70630290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative evaluation of the probiotic and antioxidant potential of indigenous Lactic Acid Bacteria 本地乳酸菌的益生菌和抗氧化能力的比较评价

Journal of Veterinary and Animal Sciences Pub Date : 2023-01-01 DOI: 10.51966/jvas.2023.54.3.763-771

S.M. Anija, U. Sreya, S.V. Aparna, A.K. Beena, J. Ligimol, R.L. Beena

引用次数: 0

Heterologous expression of Cysteine Protease 8 from Trichomonas foetus in Pichia pastoris 胎儿毛滴虫半胱氨酸蛋白酶8在毕赤酵母中的异源表达

Journal of Veterinary and Animal Sciences Pub Date : 2023-01-01 DOI: 10.51966/jvas.2023.54.1.13-20

K. Geethanjali, P. Rathnagiri, V. Kalarani

{"title":"Heterologous expression of Cysteine Protease 8 from Trichomonas foetus in Pichia pastoris","authors":"K. Geethanjali, P. Rathnagiri, V. Kalarani","doi":"10.51966/jvas.2023.54.1.13-20","DOIUrl":"https://doi.org/10.51966/jvas.2023.54.1.13-20","url":null,"abstract":"Bovine trichomonosis is one of the most neglected venereal diseases of cattle. Trichomonas foetus, the causative organism was known over decades and is responsible for severe reproductive failure. Except for a few lab-based assays, to date, there are no point-of-care diagnostics developed to screen for the presence of infectious agents in cattle. In this study, we have identified cysteine protease 8 as a suitable antigenic protein for developing sero-diagnostics. A 960 bp Tf CP8 gene was cloned into methylotrophic Pichia pastoris X-33 by homologous recombination using a pPICZαA vector for recombinant protein expression. The traditional fed-batch method of induction with methanol resulted in inconsistent expression in 48h incubation, hence a novel single batch culture with 1% methanol induction for 24h was standardised and obtained optimal recovery of approximately 36 KDa recombinant protein secreted into media. To the best of our knowledge, this is the first report of cloning and expression of genes from Trichomonas foetus. This CP8 protein could be further optimised for developing lateral flow assays and ELISA as point-of-care tools.","PeriodicalId":52568,"journal":{"name":"Journal of Veterinary and Animal Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70627955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0