Journal of Veterinary and Animal Sciences最新文献

Polymorphism study of TLR4 gene in crossbred cattle of Kerala 喀拉拉邦杂交牛TLR4基因多态性研究

Journal of Veterinary and Animal Sciences Pub Date : 2023-01-01 DOI: 10.51966/jvas.2023.54.1.1-6

{"title":"Polymorphism study of TLR4 gene in crossbred cattle of Kerala","authors":"","doi":"10.51966/jvas.2023.54.1.1-6","DOIUrl":"https://doi.org/10.51966/jvas.2023.54.1.1-6","url":null,"abstract":"The present study was conducted to identify single nucleotide variations in exon 3 region of the bovine Toll-Like Receptor gene (TLR4) associated with somatic cell score (SCS) in crossbred cattle of Kerala using polymerase chain reaction-single stranded conformational polymorphisms (PCR-SSCP) technique. Blood samples were collected from 200 lactating crossbred cattle in different farms of Kerala Veterinary and Animal Sciences University (KVASU) and field centres of ICAR- FPT scheme, KVASU. Genomic DNA was isolated and polymorphisms were identified by SSCP analysis. The 231 bp fragment of exon 3 in TLR4 gene was found to polymorphic and two genotypes viz. CC and CD were obtained with frequencies 77 per cent and 23 per cent respectively. The frequencies of C and D allele in crossbred were found to be 0.88 and 0.12. On sequencing, C to T transition had led to one non-synonymous change at 2021th position of ORF. Cattle with CC genotype were associated with significantly lower SCS (p≤0.05) as compared to CD genotype. The association between TLR4 polymorphism and SCS described in this study suggests the possible role of SNP during mastitis infection. Thus, could be employed as a useful marker for genetic selection to promote mastitis resistance in dairy cattle.","PeriodicalId":52568,"journal":{"name":"Journal of Veterinary and Animal Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70628053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of tumour associated macrophages in different histopathological types and grades of canine mammary tumours 肿瘤相关巨噬细胞在犬乳腺肿瘤中不同病理类型和分级的评价

Journal of Veterinary and Animal Sciences Pub Date : 2023-01-01 DOI: 10.51966/jvas.2023.54.1.35-42

Navya Elizabeth Sajeev, I. Sajitha, S. Devi, B. Krishna, Sudheesh S. Nair, K. Prasanna

{"title":"Evaluation of tumour associated macrophages in different histopathological types and grades of canine mammary tumours","authors":"Navya Elizabeth Sajeev, I. Sajitha, S. Devi, B. Krishna, Sudheesh S. Nair, K. Prasanna","doi":"10.51966/jvas.2023.54.1.35-42","DOIUrl":"https://doi.org/10.51966/jvas.2023.54.1.35-42","url":null,"abstract":"Canine mammary tumours (CMTs) are the second highest reported tumours in female dogs, following skin tumours. Human breast cancers (HBCs) and CMTs share common clinical and molecular features and hence, CMTs are considered as ideal models to study the different aspects of HBC. The study utilised samples from 25 CMT suspected cases presented to University Veterinary Hospitals in Thrissur district from December 2020 to October 2021. The tumour samples were analysed histopathologically and the lesions were classified. Among the 25 cases, one was identified as ductal hyperplasia, one as a benign myxoma and all the others were found to be malignant neoplasms. Malignant tumours were further categorised into different histotypes.Histological Malignancy Grading (HMG) was also done in 23 malignant CMTs and 21.74 percent were found to be of grade I, 47.83 per cent were grade II and 30.43 per cent were grade III. Majority of the malignant tumours were simple carcinomas which comprised tubulopapillary, ductal, cribriform, solid and comedocarcinomas. Highly aggressive tumours like cribriform, solid, comedo and inflammatory carcinomas belonged to higher grades, either II or III. Infiltration of tumour associated macrophages (TAMs) was studied in different histotypes and grades of CMTs. It was identified that malignant high grade CMTs had greater TAM infiltration and hence, with further validation TAMs could be effectively used in predicting prognosis and also as a therapeutic target.","PeriodicalId":52568,"journal":{"name":"Journal of Veterinary and Animal Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70628425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of carbapenem resistant Escherichia coli in cattle and pigs 牛和猪耐碳青霉烯类大肠杆菌的检测

Journal of Veterinary and Animal Sciences Pub Date : 2023-01-01 DOI: 10.51966/jvas.2023.54.2.313-321

P. Sandeep, R. Ambily, A. Irshad, S. Joseph

{"title":"Detection of carbapenem resistant Escherichia coli in cattle and pigs","authors":"P. Sandeep, R. Ambily, A. Irshad, S. Joseph","doi":"10.51966/jvas.2023.54.2.313-321","DOIUrl":"https://doi.org/10.51966/jvas.2023.54.2.313-321","url":null,"abstract":"The aim of this study was to detect the presence of carbapenem resistant Escherichia coli (E. coli) in cattle and pigs. Altogether, 100 samples were collected, 50 each from cattle and pigs including mastitic milk and faecal samples from healthy as well as diarrhoeic cattle and faecal samples and slaughter specimens from pigs. Bacteria were isolated from the samples and antibiotic sensitivity test was carried out. Eight isolates of E. coli were obtained from cattle and 15 isolates from pigs. The phenotypic resistance patterns against carbapenems (ertapenem, imipenem and meropenem) were analysed. In cattle, the four isolates from milk were observed to be sensitive to all the three carbapenems whereas, all the isolates obtained from faecal samples were resistant to all the three carbapenems. The four faecal and three intestinal isolates of E. coli from pigs showed sensitivity to all the three carbapenems. From a total of 10 faecal samples from pigs, four, three and one isolates showed resistance to ertapenem, imipenem and meropenem respectively. Among five isolates from intestine, one isolate showed resistance to ertapenem and imipenem and no isolate showed resistance to meropenem. Polymerase chain reaction (PCR) was carried out to find the presence of carbapenem resistance genes (blaSHV, blaCTX, blaTEM and blaNDM) in all the 23 isolates and the results revealed that seven (58.3 per cent) E. coli isolates carried blaSHV gene and four (33.3 per cent) contained blaCTX gene which encodes carbapenem resistant enzymes. Antibiogram using 14 commonly used antimicrobial agents revealed varied multidrug resistant patterns among the E. coli isolates.","PeriodicalId":52568,"journal":{"name":"Journal of Veterinary and Animal Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70629112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developmental competence of bovine oocytes in maturation media supplemented with follicular fluid exosomes 牛卵母细胞在补充卵泡液外泌体的成熟培养基中的发育能力

Journal of Veterinary and Animal Sciences Pub Date : 2023-01-01 DOI: 10.51966/jvas.2023.54.2.465-471

Theres Arya, R. Abhilash, C. Jayakumar, A. Amritha, T. Naicy, H. M. Harshan, M. Revathy

{"title":"Developmental competence of bovine oocytes in maturation media supplemented with follicular fluid exosomes","authors":"Theres Arya, R. Abhilash, C. Jayakumar, A. Amritha, T. Naicy, H. M. Harshan, M. Revathy","doi":"10.51966/jvas.2023.54.2.465-471","DOIUrl":"https://doi.org/10.51966/jvas.2023.54.2.465-471","url":null,"abstract":"The present study evaluated the role offollicular fluid exosomes on the developmental competence of bovine oocytes. Ovaries from slaughtered crossbred cattle were collected, all visible surface follicles aspirated, and culture-grade oocytes were subjected to further study. Exosomes were isolated from bovine follicular fluid by differential ultracentrifugation. A total of 358 oocytes selected for the study were randomly divided into two groups. Group I constituted 111 oocytes, in which normal maturation was carried. Group II constituted 247 oocytes, in which in vitro maturation (IVM) medium was supplemented with follicular fluid exosomes at 1 μL/100 μL IVM medium. Maturation was assessed after 24h of culture in a CO2 incubator maintained at 38.5°C in 95 per cent humidified atmosphere of 5 per cent CO2. Following IVM of oocytes for 24 h, in vitro fertilisation (IVF)was carried out by co-incubating with capacitated spermatozoa for 18 h and embryo culture was carried out subsequently. In group II oocytes supplemented with exosomes, a significantly higher maturation rate (p ≤0.01) (95.80 ±1.67 vs76.10 ± 0.95), fertilisation rate (53.68 ± 3.02vs37.85 ± 7.01) and cleavage rate (p ≤0.01) (43.66 ± 2.13vs(32.47 ± 5.23) was noticed compared to oocytes in group I without any supplementation.The present study established that supplementation of follicular fluid exosomescould improve the developmental competence of bovine oocytes.","PeriodicalId":52568,"journal":{"name":"Journal of Veterinary and Animal Sciences","volume":"250 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70629808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation on the prevalence of chicken anaemia virus in poultry flocks of Kerala, India 印度喀拉拉邦鸡群中鸡贫血病毒流行病学调查

Journal of Veterinary and Animal Sciences Pub Date : 2023-01-01 DOI: 10.51966/jvas.2023.54.3.687-692

P. Vidya, S. Sankar, M. Mini, P. M. Priya, I. S. Sajitha

引用次数: 0

Immunohistochemical localisation of Glutamate ammonia ligase (GLUL) in cerebrum in different clinical forms of canine rabies 不同临床形式犬狂犬病大脑谷氨酸氨连接酶(GLUL)的免疫组织化学定位

Journal of Veterinary and Animal Sciences Pub Date : 2023-01-01 DOI: 10.51966/jvas.2023.54.3.629-635

P. Nikhithasree, C. Divya, M.L. Arya Nair, K. Krithiga, K.S. Prasanna, Lali F. Anand, John Bernet Johnson

{"title":"Immunohistochemical localisation of Glutamate ammonia ligase (GLUL) in cerebrum in different clinical forms of canine rabies","authors":"P. Nikhithasree, C. Divya, M.L. Arya Nair, K. Krithiga, K.S. Prasanna, Lali F. Anand, John Bernet Johnson","doi":"10.51966/jvas.2023.54.3.629-635","DOIUrl":"https://doi.org/10.51966/jvas.2023.54.3.629-635","url":null,"abstract":"Rabies, one of the earliest known diseases reported in the history of mankind, is a fatal acute viral disease of the central nervous system. Despite numerous studies on etiology of rabies, its exact mechanism of neuropathogenesis remains unidentified. Glutamate ammonia ligase (GLUL) is a protein present in brain which play an important role in the pathogenesis of many neurological conditions. GLUL was identified to be over expressed in brain of human rabies. Cerebral cortex is the part of brain primarily responsible for coordination of movements and behaviour in animals and humans. Hence the work was designed to study the immunohistochemical localisation of GLUL in cerebrum in rabid carcasses in order to elucidate its role in the pathology of this deadly viral infection. The carcasses of 219 rabies suspected dogs that were brought to the Department of Veterinary Pathology, CVAS, Mannuthy, for necropsy between January 2021 and August 2022 formed the materials for the study. One hundred and thirty-three cases were found positive for rabies using the gold standard test for rabies - Direct Fluorescent Antibody Test (dFAT) which was further confirmed by polymerase chain reaction targeting N gene with amplicon size 533 bp. Among these 133 confirmed cases, 30 selected samples (frontal, occipital, temporal, parietal lobes of cerebrum) were further processed for histopathological and immunohistochemical (IHC) studies. The IHC signals of GLUL obtained were compared with the clinical form of rabies. Immunohistochemical staining revealed localisation of GLUL in glial cells with different staining intensity in encephalitic and paralytic form of rabies. The study concluded that the IHC signals for GLUL were stronger for furious form of rabies and weak for dumb form of the disease.","PeriodicalId":52568,"journal":{"name":"Journal of Veterinary and Animal Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136304283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dimethylacetamide - an alternative to glycerol as cryoprotectant of Malabari buck semen 二甲基乙酰胺——一种替代甘油作为马拉巴利雄鹿精液冷冻保护剂的方法

Journal of Veterinary and Animal Sciences Pub Date : 2023-01-01 DOI: 10.51966/jvas.2023.54.1.64-70

{"title":"Dimethylacetamide - an alternative to glycerol as cryoprotectant of Malabari buck semen","authors":"","doi":"10.51966/jvas.2023.54.1.64-70","DOIUrl":"https://doi.org/10.51966/jvas.2023.54.1.64-70","url":null,"abstract":"The key role of a cryoprotectant is to minimize the chemical and physical stress which occurs during cooling, freezing and thawing of semen. The difference between the cryoprotectant (CPA) occurs in their permeability coefficient and the structural model of the cryogenic agent. The beneficial effect of dimethylacetamide (DMA) as a cryoprotectant especially for sperms had been observed in several studies. The aim of the study was to study the cryoprotective effect of DMA in freezing the Malabari buck semen compared to glycerol. Ten ejaculates were taken from fourMalabaribucks . After preliminary evaluation sample split technique was followed with Tris based extender containing glycerol (6.7 per cent) as cryoprotectant (control) and Trisextender containing DMA (3 per cent) as cryoprotectant (treatment group). The semen straws (0.25mL) after filling were subjected for equilibration and manual freezing. Sperm kinetics was studied using computer-aided sperm analyzer. Pre-freeze and post-thaw evaluation included sperm viability, sperm abnormality, hypo osmotic test, acrosome integrity test and DNA fragmentation. Results indicated that inclusion of 6.7 per cent glycerol had significantly higher (p<0.05) post-thaw values than DMA. From our study we conclude that 6.7 per cent glycerol was better than 3 per cent DMA in cryopreservation of Malabari buck semen.","PeriodicalId":52568,"journal":{"name":"Journal of Veterinary and Animal Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70628588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of nutrient digestibility in four dry dog foods containing different levels of protein, fat and carbohydrate 四种含有不同水平蛋白质、脂肪和碳水化合物的狗粮的营养物质消化率评估

Journal of Veterinary and Animal Sciences Pub Date : 2023-01-01 DOI: 10.51966/jvas.2023.54.2.348-353

Prem Akhil, K. Ally, K. Ajith, Rani Jasmine, T. Sathu

{"title":"Assessment of nutrient digestibility in four dry dog foods containing different levels of protein, fat and carbohydrate","authors":"Prem Akhil, K. Ally, K. Ajith, Rani Jasmine, T. Sathu","doi":"10.51966/jvas.2023.54.2.348-353","DOIUrl":"https://doi.org/10.51966/jvas.2023.54.2.348-353","url":null,"abstract":"A study was undertaken in switch over design to find out the effects of four different treatment combinations of protein, fat and carbohydrates in dry dog foods on the apparent digestibility of nutrients. Four medium sized healthy adult nondescript dogs of about one to two years of age and around 10 to 15 kg body weight were selected and allotted to four dietary treatments- T1 (40 per cent CP, 23 per cent fat, 30 per cent carbohydrate and 3626 kcal ME), T2 (35 per cent CP, 20 per cent fat, 38 per cent carbohydrate and 3528 kcal ME), T3 (26 per cent CP, 15 per cent fat, 52 per cent carbohydrate and 3475 kcal ME) and T4 (20 per cent CP, 11 per cent fat 62 per cent carbohydrate and 3370 kcal ME). The feeding trial was conducted in switch over model of design with one week in between the periods to nullify carry over effect. Results revealed that levels of nutrients in diet had a significant influence on nutrient digestibility in the experimental animals. Among the dietary treatments, T1 with higher fat and protein levels had better dry matter digestibility while T3 diet containing 26 per cent protein, 15 per cent fat and 52 per cent carbohydrate had better crude protein and ether extract digestibility, hence can be recommended.","PeriodicalId":52568,"journal":{"name":"Journal of Veterinary and Animal Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70628999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of response to combination therapy with enalapril and torasemide in dogs with mitral valve disease 依那普利和托拉塞米联合治疗二尖瓣疾病的疗效评价

Journal of Veterinary and Animal Sciences Pub Date : 2023-01-01 DOI: 10.51966/jvas.2023.54.2.374-381

J. Greeshma, S. Rajan, U. Pillai, N. Unny, V. Gleeja

{"title":"Evaluation of response to combination therapy with enalapril and torasemide in dogs with mitral valve disease","authors":"J. Greeshma, S. Rajan, U. Pillai, N. Unny, V. Gleeja","doi":"10.51966/jvas.2023.54.2.374-381","DOIUrl":"https://doi.org/10.51966/jvas.2023.54.2.374-381","url":null,"abstract":"The present study was carried out with the objective of evaluating the response to a combination therapy of enalapril and torasemide in dogs with mitral valve disease (MVD). Dogs diagnosed with stage C of MVD as per the American College of Veterinary Internal Medicine guidelines were included in the study. Treatment was initiated with enalapril at 0.5 mg/kg BID and torasemide at 0.2 mg/kg OD orally on 0th day. Detailed clinical examination with special reference to the cardiovascular system including measurement of blood pressure, radiographic, electrocardiographic and echocardiographic parameters of the animals were performed on 0th and 30th day of treatment. The treatment was well tolerated by all the animals. Amelioration of clinical signs with a noticeable reduction in cough was noticed in all the animals. On 30th day of treatment, a significant decrease was noticed in the vertebral heart score and left ventricular internal diameter during diastole and a non-significant decrease was noticed in left atrium to aortic root ratio, left ventricular internal diameter during systole, with a considerable reduction in severity of mitral regurgitation. Post- treatment clearing of lung fields was noticed in dogs with radiographic evidence of pulmonary oedema on 0th day. In addition to this, ventricular premature complexes noticed in three animals pre- treatment was not noticed post treatment.","PeriodicalId":52568,"journal":{"name":"Journal of Veterinary and Animal Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70629200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Constraints perceived by the field extension functionaries of Dairy Development Department, Kerala 喀拉拉邦乳业发展部现场推广人员所感受到的限制

Journal of Veterinary and Animal Sciences Pub Date : 2023-01-01 DOI: 10.51966/jvas.2023.54.1.130-137

B. Akhila, S. K. Mohan, R. Jiji, A. George, A. George

{"title":"Constraints perceived by the field extension functionaries of Dairy Development Department, Kerala","authors":"B. Akhila, S. K. Mohan, R. Jiji, A. George, A. George","doi":"10.51966/jvas.2023.54.1.130-137","DOIUrl":"https://doi.org/10.51966/jvas.2023.54.1.130-137","url":null,"abstract":"The study was conducted in Kerala state in 2021–2022, with the objective of identifying the constraints related to effective role performance as perceived by the Field Extension Functionaries (FEFs) of the Dairy Development Department (DDD). Through non-proportionate stratified random sampling, a sample of 120 FEFs was chosen, of whom 60 were Dairy Extension Officers (DEOs) and 60 were Dairy Farm Instructors (DFIs). The study found that inadequate infrastructure facilities and lack of transport facilities were the major physical constraints perceived by the respondents. Among organisational constraints, inadequate staff strength and heavy administrative work were cited as the major constraints. The most significant technological constraints perceived were inadequate knowledge about dairy innovations among the farmers and lack of technical support for implementing schemes. Inadequate budget allocation for programme execution and lack of monitoring and evaluation of schemes were the major managerial constraints. Under communication constraints, weak research-extension-farmer linkage, and lack of feedback from farmers were perceived as the major ones.","PeriodicalId":52568,"journal":{"name":"Journal of Veterinary and Animal Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70627924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0