Journal of Veterinary and Animal Sciences最新文献

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Molecular characterisation of antimicrobial resistance in coagulase negative staphylococci isolated from bovine subclinical mastitis 牛亚临床乳腺炎凝固酶阴性葡萄球菌耐药性的分子特征
Journal of Veterinary and Animal Sciences Pub Date : 2023-01-01 DOI: 10.51966/jvas.2023.54.1.153-159
{"title":"Molecular characterisation of antimicrobial resistance in coagulase negative staphylococci isolated from bovine subclinical mastitis","authors":"","doi":"10.51966/jvas.2023.54.1.153-159","DOIUrl":"https://doi.org/10.51966/jvas.2023.54.1.153-159","url":null,"abstract":"Coagulase negative staphylococci (CNS) are emerging as the most prevalent causative agent of bovine mastitis. They are resistant to many commonly used antibiotics due to the presence of antimicrobial resistance (AMR) genes. A study was conducted to evaluate the AMR profiling of CNS isolated from bovine subclinical mastitis.Coagulase negative staphylococci were isolated from 49 (44.95 per cent) of the subclinical mastitis samples. Disc diffusion assay revealed that highest resistance was shown against gentamicin (42.85 per cent) followed by methicillin (32.6 per cent), ceftriaxone – tazobactam (24.48 per cent), enrofloxacin (20.4 per cent), tetracycline (16.32 per cent) and least resistance to cotrimoxazole (4 per cent). Genotypic characterisation of AMR genes such as mecA, aacA-aphD and norA by PCR was done for determining resistance to methicillin, gentamicin and fluroquinolone resistance. The CNS carried aacA-aphD, norA and mecA in 44.89 per cent, 32.65 per cent and 14.28 per cent, respectively. Comparison of phenotypic and genotypic characterisation of AMR in CNS was carried out by McNemar test and it was found that there was significant difference between the presence of mecA gene and methicillin resistance. There was no significant difference noticed forcharacterisation of phenotypic and genotypic AMR of CNS for gentamicin and fluroquinolone resistance.","PeriodicalId":52568,"journal":{"name":"Journal of Veterinary and Animal Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70628020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Electrocardiographic changes associated with hyperkalaemia in domestic cats 家猫与高钾血症相关的心电图变化
Journal of Veterinary and Animal Sciences Pub Date : 2023-01-01 DOI: 10.51966/jvas.2023.54.1.115-124
{"title":"Electrocardiographic changes associated with hyperkalaemia in domestic cats","authors":"","doi":"10.51966/jvas.2023.54.1.115-124","DOIUrl":"https://doi.org/10.51966/jvas.2023.54.1.115-124","url":null,"abstract":"Hyperkalaemia is a life-threatening electrolyte imbalance because it affects cardiac conduction and can lead to fatal arrhythmias if left untreated. The present study describes the occurrence of hyperkalaemia in cats and the electrocardiographic changes associated with this electrolyte imbalance. Hyperkalaemia was identified in 83.33 per cent of the study group subjects. Acute kidney injury and obstructive uropathy were the main clinical conditions associated with it. Electrocardiographic findings in hyperkalaemia in different cats under study included peaked T waves in lead II and the precordial lead CV6LL, atrial standstill and sino-ventricular rhythm, normal sinus rhythm, ventricular tachycardia, first-degree atrio-ventricular block, bradycardia, sinus tachycardia, and atrio-ventricular dissociation. Electrocardiography should always be performed in cases suspected of electrolyte imbalances, particularly hyperkalaemia, so as to identify any fatal arrhythmias and initiate treatment at the earliest.","PeriodicalId":52568,"journal":{"name":"Journal of Veterinary and Animal Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70628297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of feeding low protein diets supplemented with selected essential amino acids on growth performance and gut health in early weaned Large White Yorkshire piglets 饲粮中添加特定必需氨基酸对早期断奶大白约克夏仔猪生长性能和肠道健康的影响
Journal of Veterinary and Animal Sciences Pub Date : 2023-01-01 DOI: 10.51966/jvas.2023.54.2.537-543
Nikhila Mohan, M. Dipu, K. Ally, A. Usha, J. Mathew
{"title":"Effect of feeding low protein diets supplemented with selected essential amino acids on growth performance and gut health in early weaned Large White Yorkshire piglets","authors":"Nikhila Mohan, M. Dipu, K. Ally, A. Usha, J. Mathew","doi":"10.51966/jvas.2023.54.2.537-543","DOIUrl":"https://doi.org/10.51966/jvas.2023.54.2.537-543","url":null,"abstract":"A study was conducted to assess the effect of feeding low protein diets supplemented with selected amino acids on growth performance and gut health in early weaned Large White Yorkshire piglets. Thirty Large White Yorkshire female piglets belonging to Centre for Pig Production and Research, Mannuthy, weaned at 21 days of age were allotted to three dietary treatment groups (T1, T2 and T3) with five replicates for each treatment. Piglets were fed pre-starter ration till they gained a body weight of 5 kg and then on starter ration from 5 to 18 kg body weight. The dietary protein in treatments T2 and T3 were reduced by 15 and 25 per cent respectively than that of control treatment (T1). Five amino acids viz., lysine, methionine, threonine, valine and isoleucine were supplemented to all treatment groups to meet the requirements as per NRC (1998). Body weights of animals were taken at weekly intervals. There was no significant difference in average body weight among three treatment groups from first to third week. From fourth to eleventh week, the average weekly body weight of T1 was significantly higher than T3. The average daily gain was significantly different among three treatment groups with highest values observed for group T1, followed by T2 and T3. The feed conversion efficiency was statistically similar among treatment groups. Total viable count and coliform count were significantly lower in the faeces of piglets fed with low protein diets, indicating a reduction in microbial population in faeces of piglets in relation to reduction in dietary protein level. From the overall results, it could be concluded that reducing the dietary protein levels had resulted in reduced growth performance in early weaned piglets, in spite of being supplemented with selected essential amino acids","PeriodicalId":52568,"journal":{"name":"Journal of Veterinary and Animal Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70629443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isolation of acid soluble collagen from daggertooth pike conger (Muraenesox cinereus) and evaluation of its antimicrobial activity 剑齿虎酸溶性胶原蛋白的分离及抑菌活性评价
Journal of Veterinary and Animal Sciences Pub Date : 2023-01-01 DOI: 10.51966/jvas.2023.54.2.437-442
Nimna Ajay, V. P. Panicker, R. Uma, P. Divya, S. S. Devi
{"title":"Isolation of acid soluble collagen from daggertooth pike conger (Muraenesox cinereus) and evaluation of its antimicrobial activity","authors":"Nimna Ajay, V. P. Panicker, R. Uma, P. Divya, S. S. Devi","doi":"10.51966/jvas.2023.54.2.437-442","DOIUrl":"https://doi.org/10.51966/jvas.2023.54.2.437-442","url":null,"abstract":"The study was conducted to isolate acid soluble collagen (ASC) from daggertooth pike conger eel and to evaluate the antimicrobial activity of the isolated protein. Conger eels were collected from Kochi harbour. The proximate composition of eel skin consisted of 68.73 per cent of moisture on a wet basis with a crude protein, crude fat and total ash of 30.24 per cent, 12.17 per cent and 2.16 per cent respectively on a dry matter basis. Conger eel skin collagen was isolated by treating with 0.5 M acetic acid after pretreatment with 0.1 N NaOH and 10 per cent butanol. Salting out and precipitation of ASC was carried out by adding NaCl to a final concentration of 2.3 M. Precipitated ASC was then dialysed against 0.1 M acetic acid for 24h and then in distilled water until neutral pH was obtained. The yield of ASC was 12.78 per cent on a wet basis and 31.95 per cent on a dry basis. The antimicrobial activity of ASC was determined by the agar diffusion method. ASC showed antimicrobial activity against both E. coli and S. aureus.","PeriodicalId":52568,"journal":{"name":"Journal of Veterinary and Animal Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70629564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of Vitamin C enriched Amla Whey drink 富含维生素C的Amla乳清饮料的研制
Journal of Veterinary and Animal Sciences Pub Date : 2023-01-01 DOI: 10.51966/jvas.2023.54.2.571-578
K. Rashmi, H. G. R. Rao
{"title":"Development of Vitamin C enriched Amla Whey drink","authors":"K. Rashmi, H. G. R. Rao","doi":"10.51966/jvas.2023.54.2.571-578","DOIUrl":"https://doi.org/10.51966/jvas.2023.54.2.571-578","url":null,"abstract":"Chhana whey is a nutritious by product of dairy industry and its usage for formulation of whey beverages will facilitate its economic utilisation and prevent environmental pollution. Amla or Aonla is the richest source of vitamin C with many health benefits. In the present study Amla juice was used to enrich vitamin C content and enhance nutritional value of chhana whey. Amla whey drink was prepared by blending amla juice at 5, 10 and 15 per cent of chhana whey. Sugar was added at 10 per cent. Based on sensory evaluation and physico- chemical characteristics like pH, acidity, ascorbic acid, total soluble solids, total solids, viscosity the best level was selected. The effect of different heat treatments like 63°C/ 30 min, 72°C/1 min, 80°C/5 min on sensory characteristics and vitamin C stability of Amla whey drink was also analysed. The beverage prepared from 10 per cent blend had highest overall acceptability scores (8.27). There was 5 times increase in ascorbic acid content of whey when amla juice was added at 10 per cent. The heat treatment at temperature of 72°C /1min was found to be the best as it retained more ascorbic acid.","PeriodicalId":52568,"journal":{"name":"Journal of Veterinary and Animal Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70630046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Differentiation of amphistome species of cattle in Kerala by polymerase chain reaction - restriction fragment length polymorphism 聚合酶链反应-限制性片段长度多态性对喀拉拉邦牛类的鉴别
Journal of Veterinary and Animal Sciences Pub Date : 2023-01-01 DOI: 10.51966/jvas.2023.54.3.722-728
K. Thulasi, K. Devada, H. Shameem, Naicy Thomas, K. K. Jayavardhanan
{"title":"Differentiation of amphistome species of cattle in Kerala by polymerase chain reaction - restriction fragment length polymorphism","authors":"K. Thulasi, K. Devada, H. Shameem, Naicy Thomas, K. K. Jayavardhanan","doi":"10.51966/jvas.2023.54.3.722-728","DOIUrl":"https://doi.org/10.51966/jvas.2023.54.3.722-728","url":null,"abstract":"The present study evaluated the PCR-RFLP of ITS-2+genes for species differentiation of three major amphistomes namely Gastrothylax crumenifer, Fischoederius cobboldi and Paramphistomum spp. Molecular analysis using PCR yielded amplicons of 515 bp for each species. The nucleotide differences among the sequences of the three species of amphistomes at different positions were further used for designing suitable RFLP. The amphistome parasites were distinguished taking into account the differences in the recognition sequences of TspRI on the ITS-2+ region by PCR-RFLP. The enzyme cleaved F.cobboldi at two recognition sites, CAGTG and CACTG and yielded 331, 93 and 91 bp products. Gastrothylax crumenifer had one recognition site CAGTG and resulted in 331 bp and 184 bp fragments. Since no recognition sequence was found in Paramphistomum spp., there was no cleavage. It is concluded that PCRRFLP was a promising molecular tool for species identification of amphistomes.","PeriodicalId":52568,"journal":{"name":"Journal of Veterinary and Animal Sciences","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136304284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Green synthesis of silver nanoparticles from aqueous extract of leaves of Azadirachta indica, its phytochemical screening and antibacterial activity 印楝叶水提物绿色合成纳米银、植物化学筛选及抗菌活性研究
Journal of Veterinary and Animal Sciences Pub Date : 2023-01-01 DOI: 10.51966/jvas.2023.54.3.647-655
Miyada Yoosuf, Deepa Jolly, C. Latha, C. Sethulekshmi, D. K. Deepak Mathew
{"title":"Green synthesis of silver nanoparticles from aqueous extract of leaves of Azadirachta indica, its phytochemical screening and antibacterial activity","authors":"Miyada Yoosuf, Deepa Jolly, C. Latha, C. Sethulekshmi, D. K. Deepak Mathew","doi":"10.51966/jvas.2023.54.3.647-655","DOIUrl":"https://doi.org/10.51966/jvas.2023.54.3.647-655","url":null,"abstract":"Silver nanoparticles are gaining importance on account of its antimicrobial properties and low toxicity to humans. The present study was conducted to synthesise silver nanoparticles (AgNPs) from aqueous extract of neem leaves. The phytochemical screening of the aqueous extract of neem leaves was also performed for determining its active principles. The qualitative phytochemical screening revealed that the aqueous extract of neem leaves contained phytochemicals like steroids, alkaloids, phenolic compounds, tannins, flavonoids, saponins and terpenoids. The AgNPs synthesised were characterised using X-ray diffraction analysis which revealed that synthesised AgNPs were face centred, cubic and crystalline in nature and the sample contained silver nanoparticles in a high concentration. The antibacterial activity of the green AgNPs against E. coli isolates including the reference strain was tested and observed that the AgNPs exhibited antibacterial activity though a significant zone of inhibition was not obtained. Based on the results obtained, it could be concluded that silver nanoparticles could be efficiently synthesised from neem leaves and could be utilised in various fields especially in nanotechnology.","PeriodicalId":52568,"journal":{"name":"Journal of Veterinary and Animal Sciences","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136301819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of intravenous lignocaine on anaesthetic parameters in cattle under dexmedetomidine-butorphanol-ketaminemidazolam- isofluraneanaesthesia 静脉注射利多卡因对右美托咪定-布托啡诺-氯胺酮咪唑仑-异氟烷麻醉下牛麻醉参数的影响
Journal of Veterinary and Animal Sciences Pub Date : 2023-01-01 DOI: 10.51966/jvas.2023.54.1.198-203
V. Jayakrishnan, S. S. Nair, R. Varghese, S. Ramankutty, Preethy John, K. Martin
{"title":"Effects of intravenous lignocaine on anaesthetic parameters in cattle under dexmedetomidine-butorphanol-ketaminemidazolam- isofluraneanaesthesia","authors":"V. Jayakrishnan, S. S. Nair, R. Varghese, S. Ramankutty, Preethy John, K. Martin","doi":"10.51966/jvas.2023.54.1.198-203","DOIUrl":"https://doi.org/10.51966/jvas.2023.54.1.198-203","url":null,"abstract":"The present study was conducted for clinical evaluation of a multimodal anaesthetic protocol using butorphanol-dexmedetomidine-midazolam-ketamine-isoflurane and lignocaine continuous rate infusion (CRI) in six cross-bred female cattle. Preanaesthetics used were butorphanol and dexmedetomidine which were administered intravenously at dose rates of 0.05 mg/kg and 1 μg/ kg body weight, respectively. Ketamine and midazolam were intravenously administered at dose rates of 4.0 and 0.2 mg/kg body weight respectively, to induce anaesthesia. Isoflurane was used to maintain anaesthesia after endotracheal intubation, at a concentration of 1.05 ± 0.97 per cent concentration in 100 per cent oxygen using a large animal anaesthesia machine. Simultaneously, intravenous lignocaine was administered at a bolus dose of 2 mg/kg body weight followed by a CRI of 3 mg/kg body weight/hour using a volumetric infusion pump. Isoflurane sparing effect of intravenous lignocaine reduced the required concentration of isoflurane for maintenance. The third plane of surgical anaesthesia was maintained and various surgical procedures were done. Recovery was smooth. Other than the mild regurgitation of ruminal fluid in an animal, no anaesthetic complications were noticed.","PeriodicalId":52568,"journal":{"name":"Journal of Veterinary and Animal Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70628402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pyothorax in a cat due to Acinetobacter spp. infection : a case report 猫因不动杆菌感染致脓胸1例
Journal of Veterinary and Animal Sciences Pub Date : 2023-01-01 DOI: 10.51966/jvas.2023.54.1.229-233
A. Jameel, K. Davis, A. George, K. V. Kumar, V. J. Udayasree
{"title":"Pyothorax in a cat due to Acinetobacter spp. infection : a case report","authors":"A. Jameel, K. Davis, A. George, K. V. Kumar, V. J. Udayasree","doi":"10.51966/jvas.2023.54.1.229-233","DOIUrl":"https://doi.org/10.51966/jvas.2023.54.1.229-233","url":null,"abstract":"A 3-month-old kitten weighing 1.05 kg was presented to the University veterinary hospital at Kokkalai in Thrissur district of Kerala, India, with the complaint of open-mouth breathing, lethargy and complete anorexia for the past three days, and weight loss. Detailed clinical examination revealed inspiratory dyspnoea without stridor, tachypnoea, muffled lung and heart sounds on auscultation, pale mucous membrane, and weak pulse. Haematology revealed leukocytosis with granulocytosis and monocytosis. Thoracic radiography, ultrasonography and thoracocentesis confirmed pyothorax. Microbial culture of thoracic aspirate revealed heavy growth of Acinetobacte spp. The cat responded to parenteral therapy with ceftiofur followed by cefixime orally for 21 days. Doxycycline was prescribed for concurrent mycoplasmosis. Thoracic radiograph 10 days later revealed a significant reduction in pleural fluid. A review of the case two months later found that the kitten recovered uneventfully. A rare case of Acinetobacter spp. associated pyothorax and its medical management in a cat is reported. The clinical manifestations, radiographic changes and ultrasonographic findings were discussed.","PeriodicalId":52568,"journal":{"name":"Journal of Veterinary and Animal Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70628710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Current Status of Antimicrobial Use in Animals 动物抗微生物药物使用现状
Journal of Veterinary and Animal Sciences Pub Date : 2023-01-01 DOI: 10.51966/jvas.2023.54.2.283-298
Sushim K Gupta, R. Biggs, A. Ramachandran
{"title":"Current Status of Antimicrobial Use in Animals","authors":"Sushim K Gupta, R. Biggs, A. Ramachandran","doi":"10.51966/jvas.2023.54.2.283-298","DOIUrl":"https://doi.org/10.51966/jvas.2023.54.2.283-298","url":null,"abstract":"Antimicrobials are used for preventing and treating infectious diseases in animals and humans. In animals they are also commonly used to improve productivity and welfare. The overuse or misuse of antimicrobials can contribute to the development of antimicrobial resistance (AMR), which can have serious implications on both human and animal health. It is important to increase AMR awareness and coordinated surveillance at the global, continental, and national levels to quantify antimicrobial usage and monitor AMR spread. This will help to formulate better and effective strategies and policies for the judicious use of antimicrobials and combat AMR.","PeriodicalId":52568,"journal":{"name":"Journal of Veterinary and Animal Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70629280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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