Sovremennye tekhnologii v meditsine最新文献

{"title":"The Data-Driven Definition of \"Complication\" in Medicine.","authors":"G V Danilov, A A Kotov, D Yu Usachev, A G Nazarenko, Yu V Strunina, T V Tsukanova, K B Kotik, A A Potapov","doi":"10.17691/stm2025.17.4.02","DOIUrl":"10.17691/stm2025.17.4.02","url":null,"abstract":"<p><p>The concept of \"complication\" is widely used in the medical domain to designate unfavorable events in the course of medical care. However, the medical community has not yet established a strict and generally accepted definition of \"complication\". This makes it much harder to systematically track and ensure the safety of medical care, whether in an individual clinic or across the entire healthcare system. <b>This study aimed</b> to define the concept of \"complication\" by identifying its generic concept and key distinguishing features using natural language processing.</p><p><strong>Results: </strong>We conducted linguistic and statistical analysis of the term \"complication\" using a large corpus of medical texts from 90,688 completed neurosurgical cases in the digital archive of the N.N. Burdenko National Medical Research Center for Neurosurgery, Ministry of Health of the Russian Federation, spanning 2000 to 2017. The corpus was tokenized and normalized to obtain a vocabulary of 40,121 lexemes. A total of 5853 lexemes were selected as the lexicon of adverse medical events (LAME), supposed to be found in the context of complications. Using n-gram vector representations trained on our corpus, we obtained vector representations of LAME words and selected 4416 words as the sub-LAME core based on their positive cosine similarity with the vector for \"complication\". From the nouns, adjectives, and verbs in the sub-LAME, we extracted features that generalize, characterize, and classify complications. \"Pathology\" was identified as the generic concept for complication. The distinguishing features of complications were determined to be their novelty and emergence during observation of a primary phenomenon.Thus, we propose the following definition of \"complication\" for medical care safety monitoring:<i>A complication (in medicine) is an intercurrent pathology detected during observation of an underlying disease, physiological process, or the result of intervention</i>.Our patented method presented in this paper enables the development of scientifically grounded definitions for unclear or poorly defined concepts.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"17 4","pages":"19-31"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12715485/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145807292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Scale for Predicting the No-Reflow Phenomenon in Myocardial Infarction.","authors":"I G Pochinka, A A Frolov, K V Kuzmichev, N A Shchelchkova, V I Pershin, N S Maximova, M L Budkina, I V Predeina, I A Frolov, M G Kashtanov","doi":"10.17691/stm2025.17.5.05","DOIUrl":"10.17691/stm2025.17.5.05","url":null,"abstract":"<p><p><b>The aim of the study is</b> to investigate the association of the selected single nucleotide polymorphisms (SNPs) with the development of the no-reflow phenomenon during percutaneous coronary intervention (PCI) in patients with ST-segment elevation myocardial infarction (STEMI) and to create a genetic scale for predicting this complication.</p><p><strong>Materials and methods: </strong>A single-center matched case-control study was conducted. The study included 80 STEMI patients: 40 (50%) with no-reflow and 40 (50%) without no-reflow (1:1 matching by sex and age). No-reflow was defined as TIMI flow grade <3 or Myocardial blush grade <2 after PCI. The following SNPs were assessed: rs4961 (<i>ADD1</i>), rs699 and rs4762 (<i>AGT</i>), rs5186 (<i>AGTR1</i>), rs1403543 (<i>AGTR2</i>), rs1799998 (<i>CYP11B2)</i>, rs5443 (<i>GNB3</i>), rs2070744 and rs1799983 (<i>eNOS</i>), rs5370 (<i>EDN1</i>), rs1799963 (<i>F2</i>), rs6025 (<i>F5</i>), rs6046 (<i>F7</i>), rs5985 (F13), rs1800790 (<i>FGB</i>), rs1126643 (<i>ITGA2</i>), rs5918 (<i>ITGB3</i>), rs1799762 (<i>PAI-1</i>), rs1801133 and rs1801131 (<i>MTHFR</i>), rs1805087 (<i>MTR</i>), and rs1801394 (<i>MTRR</i>).</p><p><strong>Results: </strong>The following SNPs were associated with the development of the no-reflow phenomenon: rs4961 (genotype GT or TT) in the <i>ADD1</i> gene, rs1799998 (CC) in the <i>CYP11B2</i> gene, and rs1801133 (CC) in the <i>MTHFR</i> gene (p<0.05, McNemar's test). These SNPs were combined into a genetic prognostic scale, where 1 point was assigned for each genotype associated with no-reflow. The positive predictive value for the maximum score (3 points) was 0.91. The area under the ROC curve was 0.724 (0.611-0.838). The odds ratio for no-reflow development was 5.39 (1.09-26.66) per point (p=0.04; multivariate analysis using conditional logistic regression).</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"17 5","pages":"74-84"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12715492/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145807269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodistribution of the Bombesin Receptor-Targeted Radiopharmaceutical Precursor BBN/C1-C2 in a Prostate Cancer Model.","authors":"E A Beloborodov, E V Iurova, D R Dolgova, A A Ermakova, D E Sugak, A N Fomin, Y V Saenko","doi":"10.17691/stm2025.17.6.03","DOIUrl":"10.17691/stm2025.17.6.03","url":null,"abstract":"<p><p>Gastrin-releasing peptide receptor (GRPR) is a G protein-coupled receptor expressed in the central nervous system, the gastrointestinal tract, the pancreas, and the adrenal cortical tissue regulating their physiological functions. In addition to normal tissues, GRPR is overexpressed in many solid cancers. At present, several radiolabeled ligands targeting GRPR have been introduced into clinical practice for cancer diagnostics and radioligand therapy. However, there were found the high uptake of radiopharmaceuticals in normal organs and low bioavailability of the drugs caused by their stability. Therefore, GRPR radioantagonists with improved proteolytic stability and longer residence time in tumor foci with low uptake in non-target organs are currently being sought to improve the therapeutic efficacy. <b>The aim of the study</b> was to analyze the biodistribution of the BBN/C1-C2 molecule created on the basis of bombesin and knottin and its accumulation dynamics in the tumor in an <i>in vivo</i> model.</p><p><strong>Materials and methods: </strong>The study analyzed the biodistribution of Cy7.5-labeled BBN/C1-C2 peptide based on bombesin and knottin U5-Sth1a and produced using solid-phase peptide synthesis. The investigation was carried out on a mouse model Nu/Nu with a prostate cancer solid tumor (PC-3 culture) transplanted into the right side, expressing GRPR, using real-time surface fluorescent imaging on days 2 and 5 after intravenous administration of the molecules under study.</p><p><strong>Results: </strong>The biodistribution analysis showed the selective binding of the BBN/C1-C2 molecule to a tumor, as well as its holding power on the tumor surface for up to 5 days without internalization into cells with low accumulation in normal organs and tissues.</p><p><strong>Conclusion: </strong>We managed to obtain a stable molecule; however, U5-Sth1a toxin tropic to ion channels was used as a scaffold, so the resulting molecule retained the domain responsible for attaching to the target channel and typical for knottin. In the future, when using the molecule as a ligand for radiotherapy, it can have a negative effect on the patient's heart due to the supplementary radiation load. In this regard, the BBN/C1-C2 molecule requires the extension study.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"17 6","pages":"25-32"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12892846/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146184096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Candidate Genetic Markers of Schizophrenia Based on Exome Sequencing Data and Its Relation to Immunological, Clinical, and Morphometric Changes in the Russian Population.","authors":"S M Rastorguev, S V Tsygankova, M A Kaidan, V L Ushakov, I K Malashenkova, S A Krynskiy, D P Ogurtsov, N A Hailov, N V Zakharova, G P Kostyuk","doi":"10.17691/stm2025.17.2.06","DOIUrl":"10.17691/stm2025.17.2.06","url":null,"abstract":"<p><p><b>The aim of the study</b> was to identify genetic markers of schizophrenia based on exome sequencing data, as well as to specify its potential relation to clinical manifestations of the disease, morphological changes in the brain and immune disorders.</p><p><strong>Materials and methods: </strong>The analyzed sample consisted of 48 patients (23 men and 25 women; average age - 31.5±7.7 years) having a confirmed diagnosis of paranoid schizophrenia.</p><p><strong>Results: </strong>140 genes with differential polymorphisms and enriched categories that may be related to the pathogenesis of schizophrenia were identified. Analysis of genes with differential frequencies of functionally significant common single nucleotide polymorphisms (SNPs) by their major functions showed that the most common were genes involved in regulation of immune system functions and development of the nervous system, as well as genes being structural components of neurons and glia involved in the perception of sensory stimuli. The findings confirm the complexity of the genetic basis of schizophrenia. Analysis of the top 10 genes containing the most differential polymorphisms specifies such genes related to schizophrenia as <i>MUC12</i> and <i>SH3KBP1</i>. The genes involved in regulation of the immune response include <i>HLA-DQB2</i> which is one of the most significantly different SNPs between the group of patients and the general population; <i>HLA-DQB2</i> SNP (rs9276572) in patients is related to the signs of dysfunction of the antiviral component of immune system, structural changes in the brain and cognitive challenges. Although most of the detected genes are unique to the sample studied, additional studies are required to confirm these genes' involvement in the pathogenesis of this disease as well as to identify the mechanisms of the disease onset and development. The rs9276572(C) polymorphism of <i>HLA-DQB2</i> requires further study as a new potential marker of immunological disorders, morphometric changes in the brain and cognitive impairment in schizophrenia. The data obtained indicate the need for personalized medicine, because the majority of genetic prerequisites are patient-specific and highlight the importance of further research to understand the genetic aspects of schizophrenia and develop innovative approaches to its diagnosis and treatment.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"17 2","pages":"55-70"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12096358/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144145207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of L1 Vertebral Anthropomorphic Model for Densitometric Phantom Improvement.","authors":"A V Petraikin, A M Mikhailova, N D Kudryavtsev, M V Cherkasskaya, V O Yastrebova, O V Omelyanskaya, Y A Vasilev","doi":"10.17691/stm2025.17.4.05","DOIUrl":"10.17691/stm2025.17.4.05","url":null,"abstract":"<p><p><b>The aim of the study</b> was to develop L1 vertebral anthropomorphic model to improve the previously developed PHK FK2 phantom.</p><p><strong>Materials and methods: </strong>The vertebra was made using 3D printing by a digital model obtained from DICOM files of the abdominal CT examination. The phantom construction consists of three layers of different X-ray density. In the base made of photopolymer resin there is a cylindrical recess filled with a plastic mixture to imitate the normal state of the spongy substance (high density), osteopenia (moderate density decrease) and osteoporosis (significant decrease in density). Mineral density is regulated by changing β-tricalcium phosphate concentration. The cortical layer is modelled by applying metal foil on the base surface.</p><p><strong>Results: </strong>In X-ray tube voltage of 120 kV, the mean square deviations of the measured X-ray density values of the vertebral body, spongy substance and the cortical layer were 12.40, 3.96, and 57.23 HU, respectively. The mineral density assessment of the spongy substance for three X-ray tube voltages (100, 120, 140 kV) showed the mean absolute error to be 7.4 mg/ml, and the mean relative error - 7.3% (variation coefficient). The correction coefficient equal 7 mg/ml was used to correct the values, and after using the coefficient the mean absolute error decreased up to 0.4 mg/ml, and the mean relative error - up to 0.4% (variation coefficient). The relative measurement errors of the ventral, medial and dorsal vertebral body dimensions were 3.6, 2.7, and 2.9%, respectively.</p><p><strong>Conclusion: </strong>The methods used in developing a vertebral model can be applied in modeling the entire range of the mineral density of bone spongy substance: from osteoporosis to norm. The developed model demonstrates high stability of X-ray characteristics and anatomical accuracy; therefore, it can be used for equipment calibration, quality control of diagnostic systems, and in the training process to demonstrate the bone structure changes.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"17 4","pages":"52-57"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12715482/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145807312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Original Mouse Models of Glioblastoma: Analysis of Pathophysiological Characteristics of Transplanted Tumor Tissue.","authors":"V V Kudelkina, M V Gulyaev, A S Khalansky, E A Miroshnichenko, O V Makarova, T Kh Fatkhutdinov, A M Kosyreva","doi":"10.17691/stm2025.17.5.03","DOIUrl":"10.17691/stm2025.17.5.03","url":null,"abstract":"<p><p><b>The aim of this study</b> was to morphologically, molecularly, and immunologically characterize two new transplantable glioblastoma (GB) tissue models, designated M2 GB and M6 GB.</p><p><strong>Materials and methods: </strong>Two new chemically induced, easily transplantable tissue mouse models of high-grade glioma have been created and characterized. M2 GB and M6 GB tissues were orthotopically transplanted to immunocompetent C57BL/6 mice. The clinical and morphological characteristics of tumor growth, as well as the intratumoral immune response and target gene expression were assessed.</p><p><strong>Results: </strong>Clinical manifestations of M2 GB and M6 GB growth in mice include motility disorders, cachexia, and priapism. Morphologically, M2 GB and M6 GB are characterized by diffuse proliferation, cellular and nuclear polymorphism, and high mitotic activity with pathological mitotic patterns corresponding to the aggressive nature of the mentioned tumors. Both tumors were significantly infiltrated with CD3⁺ T lymphocytes (~32%) and F4/80⁺ macrophages (~28-50%). M2 GB showed a higher content of F4/80⁺ macrophages compared to M6 GB. The <i>Cdkn2a</i>, <i>S100b</i>, <i>Mki67</i>, <i>Pten</i>, <i>Vegfa</i>, <i>Hif1a</i>, <i>Sox2</i>, <i>Abcb1</i>, and <i>Gfap</i> genes were overexpressed in both tumors. Expression of the <i>Cd133</i>, <i>Tp53</i>, and <i>Pdgfra</i> genes was increased in M2 GB. High expression of <i>Pi3k</i> and <i>Gdnf</i> was seen in M6 GB. Expression of <i>Cd44</i>, <i>Pi3k</i>, <i>Hif1a</i>, <i>Gdnf</i>, and <i>Egfr</i> was higher in M6 GB tissues compared to M2 GB, whereas expression of <i>Cdkn2a</i>, <i>Tp53</i>, <i>Cd133</i>, and <i>Pdgfra</i> was higher in M2 GB tissues compared to M6 GB.</p><p><strong>Conclusion: </strong>The M2 GB and M6 GB models of transplanted tissues reproduce key characteristics of human GB, including similar intracellular immune profiles, clinical and morphological features, and gene expression patterns, which are important for further research in neurological oncology. These models can be used to develop diagnostic and treatment methods and to study tumor genesis.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"17 5","pages":"50-64"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12715489/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145807296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peritumoral Brain Zone in Astrocytoma: Morphology, Molecular Aspects, and Clinical Manifestations (Review).","authors":"A S Grishin, K A Achkasova, L S Kukhnina, V A Sharova, M V Ostapyuk, K S Yashin","doi":"10.17691/stm2024.16.2.08","DOIUrl":"10.17691/stm2024.16.2.08","url":null,"abstract":"<p><p>A peritumoral brain zone is an area between a tumor and nontumorous brain tissue with tumor cell infiltration. The identification of this area is sufficiently difficult due to the lack of clear morphological or some other criteria. Besides, its dimensions may vary considerably. In the present review, we have analyzed the available data on the morphological structure and metabolism of peritumoral zone in astrocytomes, and considered the main molecular and genetic aspects and clinical manifestations. Exploration of the peritumoral zone is of great importance for determining the extent of resection to prevent recurrence and to reveal the causes and mechanisms of continued tumor growth.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 2","pages":"79-88"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11556047/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoparticles for Creating a Strategy to Stimulate Liver Regeneration.","authors":"S A Rodimova, D S Kozlov, D P Krylov, L V Mikhailova, V A Kozlova, A I Gavrina, A M Mozherov, V V Elagin, D S Kuznetsova","doi":"10.17691/stm2024.16.3.04","DOIUrl":"10.17691/stm2024.16.3.04","url":null,"abstract":"<p><p>Presently, there is a need in the developing new approaches to stimulate liver regeneration, which would make its recovery more effective after resection. Application of nanoparticles, loaded with small bioactive molecules, with their targeted delivery into the liver is a promising approach. <b>The aim of the investigation</b> is to study the interaction of nanoparticles with various types of hepatic cells on the models of liver slices and primary hepatic cell cultures using the methods of multiphoton microscopy with fluorescence lifetime imaging.</p><p><strong>Materials and methods: </strong>Nanoparticles have been synthetized from polylactide (PLA), gold (Au), and silicon (SiO₂), and characterized using scanning and transmission electron microscopy. These types of particles were labeled with a fluorescent Cy5 dye for their visualization. Liver slices and a primary hepatocyte culture were used as models for biological testing of nanoparticles. Biodistribution of the nanoparticles in the tissue and cells, their cytotoxicity, and the effect on the cell metabolism were assessed using optical bioimaging methods.</p><p><strong>Results: </strong>The silicon nanoparticles are accumulated mainly by macrophages, which generate reactive oxygen species in a large amount and impair the native metabolic state of hepatocytes. The gold nanoparticles accumulate in all types of the liver cells but possess a marked toxic effect, which is indicated by the appearance of necrotic and apoptotic cells and a sharp change in the hepatocyte metabolic state. The polylactide nanoparticles accumulate most effectively in the liver cells, preferably in hepatocytes, do not change their native metabolic state, making this type of nanoparticles most promising for creating the bioactive molecule delivery systems to stimulate liver regeneration.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 3","pages":"31-41"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11618528/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142804418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Major Mutations in <i>CFTR, SERPINA1, HFE</i> Genes in Benign Unconjugated Hyperbilirubinemia Phenotype.","authors":"A A Ivanova, N E Apartseva, A P Kashirina, E G Nemtsova, Y V Ivanova, M V Kruchinina, S A Kurilovich, V N Maksimov","doi":"10.17691/stm2024.16.4.04","DOIUrl":"10.17691/stm2024.16.4.04","url":null,"abstract":"<p><p><b>The aim of the study</b> was to search for the associations of benign unconjugated hyperbilirubinemia phenotype with rs1799945 (H63D), rs1800562 (C282Y), rs1800730 (S65C) mutations of <i>HFE</i> gene, rs113993960 (ΔF508) of <i>CFTR</i> gene, rs28929474 (PIZ), rs17580 (PIS) mutations of <i>SERPINA1</i> gene.</p><p><strong>Material and methods: </strong>The study design is case-control. The group with Gilbert's syndrome (GS) phenotype (n=414; mean age - 36.7±15.9 years; 49.8% men) was formed by gastroenterologists, and included the individuals with unconjugated hyperbilirubinemia who underwent a standard clinical examination. The individuals with known causes of unconjugated hyperbilirubinemia were excluded from the group. The control group (n=429; mean age - 38.5±14.3 years; 52.2% men) was a random sampling from DNA banks of MONICA project participants, the screening of young people aged 25-44 and a one-time study of schoolchildren in Novosibirsk (Russia). DNA was isolated by phenol-chloroform extraction or by the express method (PROBA-RAPID-GENETIKA; DNA-Technology, Moscow, Russia) from venous blood. Genotyping of groups by nucleotide sequence rs1799945 (H63D), rs1800562 (C282Y), rs1800730 (S65C) of <i>HFE</i> gene, rs113993960 (ΔF508) of <i>CFTR</i> gene, rs28929474 (PIZ), rs17580 (PIS) of <i>SERPINA1</i> gene was performed by polymerase chain reaction followed by the analysis of fragment length polymorphism on a polyacrylamide gel.</p><p><strong>Results: </strong>According to the genotypes and alleles of the variants rs1799945 (H63D), rs1800562 (C282Y), rs1800730 (S65C) of <i>HFE</i> gene, rs113993960 (ΔF508) of <i>CFTR</i> gene, rs28929474 (PIZ), rs17580 (PIS) of <i>SERPINA1</i> gene, no statistically significant differences were found between the GS group and the control group (p>0.05).</p><p><strong>Conclusion: </strong>Nucleotide sequence variants rs1799945 (H63D), rs1800562 (C282Y), rs1800730 (S65C) of <i>HFE</i> gene, rs113993960 (ΔF508) of <i>CFTR</i> gene, rs28929474 (PIZ), rs17580 (PIS) of <i>SERPINA1</i> gene, or their combinations with rs3064744 of <i>UGT1A1</i> gene were found to have no association with GS.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 4","pages":"38-44"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11773142/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143070665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A New Neuroanesthetic Protocol of Rendering Specialized Care in Treating Degenerative Lumbar Spine Diseases in High-Risk Patients: Prospective Analysis of the Results.","authors":"A A Kalinin, V Yu Goloborodko, Yu Ya Pestryakov, R A Kundubayev, M Yu Biryuchkov, A V Shchegolev, V A Byvaltsev","doi":"10.17691/stm2024.16.3.06","DOIUrl":"10.17691/stm2024.16.3.06","url":null,"abstract":"<p><p><b>The aim of the study</b> is to assess the effectiveness of a new neuroanesthetic protocol for treating degenerative lumbar spine diseases in high-risk patients.</p><p><strong>Materials and methods: </strong>Two groups of patients with a high risk of anesthesia and surgery determined by the authors' clinical decision support system (CDSS) have been prospectively studied. A new neuroanesthetic protocol was used in the experimental group (EG, n=25), while the control group (CG, n=25) underwent intravenous anesthesia based on propofol and fentanyl. Minimally invasive transforaminal lumbar interbody fusion was performed in all cases. Changes of the intraoperative mean arterial pressure and heart rate, intensity of the local pain syndrome, amount of the opiates used, presence of cognitive disorders, adverse effects of anesthesia, and surgical complications have been compared.</p><p><strong>Results: </strong>The groups were representative (p>0.05) in terms of the age-gender parameters, anthropological data, comorbid background, involvement in smoking, preoperative characteristics of the lumbar spine, as well as the level of cognitive functions. No statistically significant changes of the mean arterial pressure (p=0.17) were registered in EG patients relative to the CG (p=0.0008). Intraoperative reduction of the heart rate in patients of the CG was not noted (p=0.49) in comparison with the EG (p=0.03). In the postoperative period, the best indicators of cognitive functions on the FAB test (p=0.02) and MoCA test (p=0.03) were revealed in EG. A significantly less amount of perioperative opiates (p=0.005) at a low level of the local pain syndrome was also noted (p=0.01). The intergroup analysis has shown fewer adverse effects of anesthesia in EG compared to CG (p=0.01) with a comparable number of postoperative surgical complications (p=0.42).</p><p><strong>Conclusion: </strong>A new neuroanesthetic protocol of rendering a specialized care to patients with a high risk of anesthesia and surgery, assessed by the authors-developed CDSS, has resulted in effective elimination of the local postoperative pain syndrome, reduction of perioperative application of opioids, and stabilization of intraoperative indicators of cardiovascular activity. In addition, no postoperative cognitive disorders, anesthetic side-effects, adverse pharmacological consequences of the complex usage of non-steroidal anti-inflammatory drugs, prolonged local anesthetics, alpha-2-agonist, and non-narcotic analgesics have been registered.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 3","pages":"51-59"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11618530/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142804410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}