Sovremennye tekhnologii v meditsine最新文献

{"title":"Peritumoral Brain Zone in Astrocytoma: Morphology, Molecular Aspects, and Clinical Manifestations (Review).","authors":"A S Grishin, K A Achkasova, L S Kukhnina, V A Sharova, M V Ostapyuk, K S Yashin","doi":"10.17691/stm2024.16.2.08","DOIUrl":"10.17691/stm2024.16.2.08","url":null,"abstract":"<p><p>A peritumoral brain zone is an area between a tumor and nontumorous brain tissue with tumor cell infiltration. The identification of this area is sufficiently difficult due to the lack of clear morphological or some other criteria. Besides, its dimensions may vary considerably. In the present review, we have analyzed the available data on the morphological structure and metabolism of peritumoral zone in astrocytomes, and considered the main molecular and genetic aspects and clinical manifestations. Exploration of the peritumoral zone is of great importance for determining the extent of resection to prevent recurrence and to reveal the causes and mechanisms of continued tumor growth.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 2","pages":"79-88"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11556047/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoparticles for Creating a Strategy to Stimulate Liver Regeneration.","authors":"S A Rodimova, D S Kozlov, D P Krylov, L V Mikhailova, V A Kozlova, A I Gavrina, A M Mozherov, V V Elagin, D S Kuznetsova","doi":"10.17691/stm2024.16.3.04","DOIUrl":"10.17691/stm2024.16.3.04","url":null,"abstract":"<p><p>Presently, there is a need in the developing new approaches to stimulate liver regeneration, which would make its recovery more effective after resection. Application of nanoparticles, loaded with small bioactive molecules, with their targeted delivery into the liver is a promising approach. <b>The aim of the investigation</b> is to study the interaction of nanoparticles with various types of hepatic cells on the models of liver slices and primary hepatic cell cultures using the methods of multiphoton microscopy with fluorescence lifetime imaging.</p><p><strong>Materials and methods: </strong>Nanoparticles have been synthetized from polylactide (PLA), gold (Au), and silicon (SiO₂), and characterized using scanning and transmission electron microscopy. These types of particles were labeled with a fluorescent Cy5 dye for their visualization. Liver slices and a primary hepatocyte culture were used as models for biological testing of nanoparticles. Biodistribution of the nanoparticles in the tissue and cells, their cytotoxicity, and the effect on the cell metabolism were assessed using optical bioimaging methods.</p><p><strong>Results: </strong>The silicon nanoparticles are accumulated mainly by macrophages, which generate reactive oxygen species in a large amount and impair the native metabolic state of hepatocytes. The gold nanoparticles accumulate in all types of the liver cells but possess a marked toxic effect, which is indicated by the appearance of necrotic and apoptotic cells and a sharp change in the hepatocyte metabolic state. The polylactide nanoparticles accumulate most effectively in the liver cells, preferably in hepatocytes, do not change their native metabolic state, making this type of nanoparticles most promising for creating the bioactive molecule delivery systems to stimulate liver regeneration.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 3","pages":"31-41"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11618528/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142804418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experience of Using Neural Networks to Assess Age-Related Changes in Some Structures of the Skull and Cervical Vertebrae Based on CT Scans (Pilot Project).","authors":"G V Zolotenkova, D K Valetov, M P Poletaeva, Yu V Vassilevski","doi":"10.17691/stm2024.16.2.03","DOIUrl":"10.17691/stm2024.16.2.03","url":null,"abstract":"<p><p><b>The aim of the investigation</b> is to study the possibility of using artificial intelligence technologies for age prediction based on CT studies of some structures of the skull and cervical vertebrae.</p><p><strong>Material and methods: </strong>The study included 223 tomograms of the head and neck in sagittal projection from patients without any pathology of the studied structures. Morphometric analysis was carried out using PjaPro and Gradient programs, statistical analysis was performed by SPSS Statistics software. A fully convolutional EfficientNet-B2 neural network was used, which was trained in two stages: selection of the area of interest and solution of regression tasks.</p><p><strong>Results: </strong>Morphometric assessment and subsequent statistical analysis of the selected group of features have shown presence of the strongest correlation with age in the indicator characterizing the involution of the median atlantoaxial joint. A deep learning method using the convolutional network, which automatically selects the desired area in the image (the area of the vertebral junction), classifies the sample, and makes an assumption about the age of the unknown individual with an accuracy of 7.5 to 10.5 years has been tested.</p><p><strong>Conclusion: </strong>As a result of the study, a positive experience has been obtained indicating the possibility of using convolutional neural networks to determine the age of the unknown person, which expands the evidence base and provides new opportunities for determining group-wide personality traits in forensic medicine.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 2","pages":"29-38"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11556052/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-adhesive Properties of Epoxy-Treated Xenopericardium Modified with Polyvinyl Alcohol: <i>in vitro</i> Study of Leukocyte Adhesion in the Pulsatile Flow Model.","authors":"E A Ovcharenko, T V Glushkova, D K Shishkova, M A Rezvova, E A Velikanova, K Yu Klyshnikov, T N Akentyeva, A E Kostyunin","doi":"10.17691/stm2024.16.2.04","DOIUrl":"10.17691/stm2024.16.2.04","url":null,"abstract":"<p><p><b>The aim of the study</b> is to assess protective capabilities of the polymer coating made of polyvinyl alcohol to prevent leukocyte adhesion to epoxy-treated bovine pericardium, which is used in production of bioprosthetic heart valves.</p><p><strong>Materials and methods: </strong>Fragments of unmodified (control) and modified with polyvinyl alcohol epoxy-treated bovine pericardium were incubated in the dedicated chambers connected to a pulsatile flow system (Ibidi GmbH, Germany). During 48 h incubation was conducted in whole donor plasma containing 3<b>·</b>10⁶ of mononuclear fraction cells. To simulate plasma flow, the shear stress on the inflow and outflow sides of of bioprosthetic heart valve in the aortic position was set to 50 and 20 dynes/cm², respectively. After the experiment was completed, the surface of the studied samples was subjected to scanning electron microscopy and immunofluorescence using antibodies to the pan-leukocyte marker CD45.</p><p><strong>Results: </strong>Adhesion of leukocytes (CD45⁺) was seen for both the serous (outflow side) and fibrous (inflow side) surfaces of the control epoxy-treated bovine pericardium, whereas both surfaces of the material modified with polyvinyl alcohol were clear of immune cells. Scanning electron microscopy confirmed the adhesion of leukocytes to intact biological tissue: the cells on the surface of the control xenopericardium were of an irregular shape and formed numerous pseudopodia.</p><p><strong>Conclusion: </strong>The suggested modification of epoxy-treated bovine pericardium with polyvinyl alcohol prevents the adhesion of immune cells to the implant surface and can potentially protect bioprosthetic heart valves from immune rejection.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 2","pages":"40-44"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11556051/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142636182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A New Neuroanesthetic Protocol of Rendering Specialized Care in Treating Degenerative Lumbar Spine Diseases in High-Risk Patients: Prospective Analysis of the Results.","authors":"A A Kalinin, V Yu Goloborodko, Yu Ya Pestryakov, R A Kundubayev, M Yu Biryuchkov, A V Shchegolev, V A Byvaltsev","doi":"10.17691/stm2024.16.3.06","DOIUrl":"10.17691/stm2024.16.3.06","url":null,"abstract":"<p><p><b>The aim of the study</b> is to assess the effectiveness of a new neuroanesthetic protocol for treating degenerative lumbar spine diseases in high-risk patients.</p><p><strong>Materials and methods: </strong>Two groups of patients with a high risk of anesthesia and surgery determined by the authors' clinical decision support system (CDSS) have been prospectively studied. A new neuroanesthetic protocol was used in the experimental group (EG, n=25), while the control group (CG, n=25) underwent intravenous anesthesia based on propofol and fentanyl. Minimally invasive transforaminal lumbar interbody fusion was performed in all cases. Changes of the intraoperative mean arterial pressure and heart rate, intensity of the local pain syndrome, amount of the opiates used, presence of cognitive disorders, adverse effects of anesthesia, and surgical complications have been compared.</p><p><strong>Results: </strong>The groups were representative (p>0.05) in terms of the age-gender parameters, anthropological data, comorbid background, involvement in smoking, preoperative characteristics of the lumbar spine, as well as the level of cognitive functions. No statistically significant changes of the mean arterial pressure (p=0.17) were registered in EG patients relative to the CG (p=0.0008). Intraoperative reduction of the heart rate in patients of the CG was not noted (p=0.49) in comparison with the EG (p=0.03). In the postoperative period, the best indicators of cognitive functions on the FAB test (p=0.02) and MoCA test (p=0.03) were revealed in EG. A significantly less amount of perioperative opiates (p=0.005) at a low level of the local pain syndrome was also noted (p=0.01). The intergroup analysis has shown fewer adverse effects of anesthesia in EG compared to CG (p=0.01) with a comparable number of postoperative surgical complications (p=0.42).</p><p><strong>Conclusion: </strong>A new neuroanesthetic protocol of rendering a specialized care to patients with a high risk of anesthesia and surgery, assessed by the authors-developed CDSS, has resulted in effective elimination of the local postoperative pain syndrome, reduction of perioperative application of opioids, and stabilization of intraoperative indicators of cardiovascular activity. In addition, no postoperative cognitive disorders, anesthetic side-effects, adverse pharmacological consequences of the complex usage of non-steroidal anti-inflammatory drugs, prolonged local anesthetics, alpha-2-agonist, and non-narcotic analgesics have been registered.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 3","pages":"51-59"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11618530/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142804410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stereotactic Photodynamic Therapy of Recurrent Malignant Gliomas.","authors":"A A Rafaelian, B V Martynov, K A Chemodakova, A I Kholyavin, R S Martynov, E Yu Klimenkova, M Yu Prokudin, G V Papayan, I V Boykov, D V Svistov","doi":"10.17691/stm2024.16.2.06","DOIUrl":"10.17691/stm2024.16.2.06","url":null,"abstract":"<p><p><b>The aim of the study</b> is to assess the effectiveness and safety of stereotactic photodynamic therapy (sPDT) with 5-aminolevulinic acid (5-ALA) in patients with recurrent malignant supratentorial gliomas in functionally relevant brain areas.</p><p><strong>Materials and methods: </strong>In a retrospective single-center study the results of sPDT with 5-ALA in 10 patients (6 of 10 were male), aged 30 to 62 years (median: 51.5 years; 95% CI: 38-59 years) with recurrent malignant brain gliomas after standard therapy who underwent surgery during the period of 2020-2023 were analyzed. sPDT was conducted during 15 min using 5-ALA at a dosage of 20 mg/kg, a diode laser with a wavelength of 635 nm and power of 1 W, and the LFT-02-BIOSPEC unit (BIOSPEC, Russia). Three patients got repeated sPDT after 3, 7, and 15 months due to a relapse. The number of target points and the optimal position for intervention paths were determined according to the data of preoperative stereotactic MRI of the brain with contrast intensification using the CRW Precision stereotactic navigation system (Integra, USA) and intraoperative registration of the area with the highest intensity of protoporphyrin IX fluorescence along the path (according to fluorescence biospectroscopy).</p><p><strong>Results: </strong>Glioblastoma (grade IV, WHO) was diagnosed in 7 patients, anaplastic astrocytoma (grade III, WHO) - in 3 persons. Genetic studies were performed for 9 patients, 7 of them had tumors without the <i>IDH1</i> gene mutation. None of the patients had a combined 1p/19q deletion. The median volume of the contrast-enhancing part of the recurrent tumor was 7.95 cm³ (95% CI: 3.3-13.6 cm³). The median time to relapse after sPDT in patients with anaplastic astrocytomas and glioblastomas was 14.5 and 6.5 months, respectively. The median survival time after sPDT in patients with glioblastomas was 15.8 months (95% CI: 0.5-20.1 months), and in patients with anaplastic astrocytomas - 46.3 months (95%, CI not specified). In the early postoperative period, two patients had motor aphasia and hemiparesis, which further regressed.</p><p><strong>Conclusion: </strong>The results of a small group of patients allow to consider sPDT with 5-ALA as a promising technique to treat patients with recurrent high-grade gliomas in functionally relevant brain areas and require further prospective assessment.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 2","pages":"58-65"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11556050/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radiomics and Quantitative MDA Criteria in Breast Cancer with Bone Metastases by MRI: Examples of Calculation Algorithms and Their Practical Use.","authors":"V Steinhauer, G Hartung","doi":"10.17691/stm2024.16.3.01","DOIUrl":"10.17691/stm2024.16.3.01","url":null,"abstract":"<p><p>In the practical work of a radiologist or oncologist, especially in the context of individualized treatment, there is a need for fast and in-depth diagnostics. Radiomics and artificial intelligence can be of great help here. Quantitative and qualitative assessments obtained in this way act as decision support for the physician. <b>The aim of the study</b> is to enhance the ability of quantitative and qualitative assessment of metastatic spinal lesions in breast cancer, to better evaluate the nature of changes under the influence of therapy, in addition to MDA.</p><p><strong>Materials and methods: </strong>We used MRI data in sagittal projection for a patient diagnosed with T2N3M1 breast cancer when treated according to accepted clinical protocols. Metastases to the spine were assessed by a radiologist and by machine analysis using the described software: image internal structure extraction operators and recognition based on traditional neural networks. Fragments of the program codes used are also given.</p><p><strong>Results: </strong>The structure of metastatically changed vertebrae in sagittal projection was analysed using machine operators of image analysis. Subtle changes in structure such as several types of \"calderas\" and the pattern of change in image complexity as treatment with CDK 4/6 inhibitors were detected. Measurements were supported by metastasis recognition using neural networks, to increase the reliability of the estimates. In addition to the ability to record response to therapy, a fundamental ability to assess the degree of action compared to previous therapy was identified.</p><p><strong>Conclusion: </strong>The study showed high efficiency of using image structure analysis algorithms, good correlation of the results obtained with the radiologist's opinion and with clinical and laboratory data, and allowed to approach the analysis of subtle effects to obtain not only quantitative characteristics in addition to MDA, but also to obtain new qualitative results.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 3","pages":"5-10"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11618525/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142804419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Capabilities of Cephalometric Methods to Study X-rays in Three-Dimensional Space (Review).","authors":"I O Ayupova, A Yu Makhota, A V Kolsanov, N V Popov, M A Davidyuk, I A Nekrasov, P A Romanova, A M Khamadeeva","doi":"10.17691/stm2024.16.3.07","DOIUrl":"10.17691/stm2024.16.3.07","url":null,"abstract":"<p><p><b>The aim of the study</b> was a systematic review of modern methods of three-dimensional cephalometric analysis, and the assessment of their efficiency. The scientific papers describing modern diagnostic methods of MFA in dental practice were searched in databases PubMed, Web of Science, eLIBRARY.RU, as well as in a searching system Google Scholar by the following key words: three-dimensional cephalometry, three-dimensional cephalometric analysis, orthodontics, asymmetric deformities, maxillofacial anomalies, 3D cephalometry, CBCT. The literature analysis showed many methods of cephalometric analysis described as three-dimensional to use two-dimensional reformates for measurements. True three-dimensional methods are not applicable for practical purposes due to the fragmentary nature of the studies. There is the disunity in choosing landmarks and supporting planes that makes the diagnosis difficult and costly. The major issue is the lack of uniform standards for tree-dimensional measurements of anatomical structures of the skull, and the data revealed can be compared to them. In this regard, the use of artificial neuron networks and in-depth study technologies to process three-dimensional images and determining standard indicators appear to be promising.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 3","pages":"62-73"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11618529/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142804411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Digital Electrocardiographic Complex for Risk Stratification of Paroxysmal Atrial Fibrillation.","authors":"A V Frolov, O P Melnikova, A P Vorobiev, T G Vaikhanskaya","doi":"10.17691/stm2024.16.3.05","DOIUrl":"10.17691/stm2024.16.3.05","url":null,"abstract":"<p><p><b>The aim of the study</b> was to develop and clinically test a hardware and software system capable of identifying the predictors of the hidden forms of atrial fibrillation (AF) using 12-lead ECG data in sinus rhythm.</p><p><strong>Materials and methods: </strong>There was developed the hardware and software system \"Intecard 8.1\" to assess a set of markers for atrial electrical instability by 3-5-minute ECG recordings in sinus rhythm. The markers include P-wave amplitude in lead II <0.1 mV, P-wave duration >120 ms, advanced interatrial block, the area of the biphasic P-wave terminal part <-4 mV·ms, and MVP (morphology-voltage- P-wave duration) score >3 points.The clinical testing of \"Intecard 8.1\" system was carried out on 120 patients with ischemic heart disease or dilated cardiomyopathy. The patients' average age was 57.9±13.1 years.</p><p><strong>Results: </strong>P-wave detection is a challenging task due to a low signal amplitude, noise, high error probability in atrioventricular block or T-wave and P-wave superposition in case of marked tachycardia. To improve detection, a phase transformation method was used, according to which there was studied its phase component arctg[<i>x</i>(<i>n</i>)<i>/Rv</i>], where <i>x</i>(<i>n</i>) <i>-</i> ECG signal samples, <i>Rv -</i> a constant. We developed an identification algorithm implemented in \"Intecard 8.1\" software, its clinical trials being conducted.During the 12 [6; 22] month observation period, AF episodes were recorded in 22 from 120 patients (18.3%). The patients with AF episodes exhibited a significant decrease in P-wave amplitude (p=0.029), its duration increase (p<0.001), and a significantly high MVP score (p<0.01). The MVP score with a cut-off point >3 points is of the highest prognostic significance. The area under the ROC curve AUC was 0.988 with a 95% confidence interval: 0.975-0.999 (p<0.001). The prediction model of hidden AF paroxysms has sensitivity and specificity: 92 and 89%, respectively.</p><p><strong>Conclusion: </strong>The digital electrocardiographic complex \"Intecard 8.1\" when analyzing 3-5-minute ECG recordings with sinus rhythm enables to identify the patients with high risk or with hidden AF forms. The dynamic assessment of P-wave parameters offers an opportunity to personalize heart rhythm control in this patient cohort.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 3","pages":"43-48"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11618526/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142804412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Technology of Combined Identification of Macrophages and Collagen Fibers in Liver Samples.","authors":"I A Nikitina, V A Razenkova, E A Fedorova, O V Kirik, D E Korzhevskii","doi":"10.17691/stm2024.16.3.03","DOIUrl":"10.17691/stm2024.16.3.03","url":null,"abstract":"<p><p>The importance of identifying pathological changes in the liver both in fundamental researches and in diagnostic practice dictates the necessity to have a convenient method of assessing functional condition of resident macrophages and connective tissue fibers. <b>The aim of the study</b> is to assess the technology of combined histo-immunohistochemical detection of collagen fibers of connective tissue and resident liver macrophages using aniline blue histological stain and available antibodies to the microglial marker, the Iba-1 protein.</p><p><strong>Materials and methods: </strong>Liver samples from adult rats (n=6) have been used in the study. The connective tissue was stained with a 2% aqueous solution of aniline blue (Unisource Chemicals Ltd., India). Monoclonal rabbit antibodies to Iba-1 (Clone JM36-62; ET1705-78; HuaBio, China) were used to detect resident liver macrophages, zinc-ethanol-formaldehyde was employed as a fixative.</p><p><strong>Results: </strong>The combined staining method allowed us to detect numerous Iba-1-immunopositive structures corresponding morphologically to Kupffer cells and connective tissue macrophages, background staining was not observed. Staining with aniline blue in the liver samples was selective, uniform, and clear, and allowed for differentiation of the connective tissue in all examined samples. Exclusion of the heat-induced epitope retrieval stage caused no negative effect on identification of macrophages, reduced the probability of non-specific staining of the collagen fibers with aniline blue, and ensured general preservation of tinctorial properties of the liver tissue.</p><p><strong>Conclusion: </strong>The presented protocol of combined histo-immunohistochemical identification of Kupffer cells and connective tissue fibers, applied on the rat liver samples, makes it possible to perform effectively the morphometric analysis and may find its application in pathohistological, clinical, and preclinical investigations.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 3","pages":"24-29"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11618527/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142804420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}