Sovremennye tekhnologii v meditsine最新文献

{"title":"Redox-Sensitive Fluorescent Nanoparticles for Biovisualization of Malignant Tumors.","authors":"O Peltek, E A Kopoleva, M V Zyuzin","doi":"10.17691/stm2025.17.1.05","DOIUrl":"10.17691/stm2025.17.1.05","url":null,"abstract":"<p><p>Application of fluorescent redox-sensitive nanoparticles in current biomedicine ensures high sensitivity and accuracy of biovisualization. Nanoparticles are potent as they can long circulate in the blood, where the level of glutathione is relatively low, and are destroyed in tumor cells, releasing loaded dyes or drugs. <b>The aim of the study</b> was to develop new nanoparticles based on trithiocyanuric acid for biovisualization of malignant tumors and study capabilities of the developed nanoparticles.</p><p><strong>Materials and methods: </strong>Nanoparticles were obtained by polycondensation of trithiocyanuric acid using iodine. Scanning and transmission electron microscopy was used for their characterization, the loading of fluorescent dyes was assessed by means of spectrophotometry. Confocal laser scanning microscopy was applied to study the impact of nanoparticles on the viability of the 4T1 and A549 cell lines as well as their interaction with cells. The distribution of nanoparticles in tissues and organs of BALB/c model mice with grafted tumors was performed using fluorescence visualization.</p><p><strong>Results: </strong>According to scanning microscopy, the size of the synthesized particles reached 100±20 nm. The adsorption isotherm demonstrated that adsorption of 0.27 mg of the RhB fluorescent dye per 1 mg of nanoparticles could be achieved. Enhanced release of the packed fluorescent dye was seen in the presence of glutathione and acetylcysteine. The particles did not significantly affect the viability of 4T1 and A549 cells. After intratumoral administration, they ensured a more intense fluorescent signal in the tumor area compared to a regular fluorescent dye solution.</p><p><strong>Conclusion: </strong>The developed system of trithiocyanuric-acid-based nanoparticles demonstrated high efficiency in biovisualization of malignant tumors and has a potential for targeted delivery of treatment agents.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"17 1","pages":"50-56"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11892567/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143607753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impact of Silicon Nanoparticle Porosity on Their Ability to Sensitize Low-Intensity Medical Ultrasound.","authors":"L A Osminkina, P A Tyurin-Kuzmin, M V Sumarokova, A A Kudryavtsev","doi":"10.17691/stm2025.17.1.04","DOIUrl":"10.17691/stm2025.17.1.04","url":null,"abstract":"<p><p>This study investigates the role of porosity in silicon nanoparticles' ability to act as sonosensitizers for sonodynamic therapy of malignant tumors. Structural analysis showed that porous nanoparticles are composed of nanocrystals approximately 4 nm in size and contain 15 nm pores, whereas non-porous nanoparticles have a dense structure with nanocrystals ranging from 10 to 50 nm. Porous nanoparticles exhibit pronounced photoluminescent properties, associated with quantum confinement effects in their small nanocrystals. The cytotoxicity of the nanoparticles was investigated in vitro using Hep2 cells. The results showed that both porous and non-porous nanoparticles in the studied concentration range (2-500 μg/ml) are non-toxic. Low-intensity ultrasound (0.88 MHz, <1 W) also does not have a toxic effect on the cells. However, the combined use of porous nanoparticles and ultrasound led to a significant decrease in cell viability, which was not observed when non-porous nanoparticles were used. This effect is associated with mechanical destruction of the cell membranes, as well as the potential activation of additional cell death mechanisms, such as apoptosis. The results highlight the importance of porosity as a key factor determining the effectiveness of silicon nanoparticles as sonosensitizers. The high efficiency, low toxicity, and unique structural properties of porous nanoparticles make them a promising material for further research and development of targeted, non-invasive treatments for malignant tumors in the context of sonodynamic therapy.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"17 1","pages":"40-47"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11892568/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143607760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoparticles for Creating a Strategy to Stimulate Liver Regeneration.","authors":"S A Rodimova, D S Kozlov, D P Krylov, L V Mikhailova, V A Kozlova, A I Gavrina, A M Mozherov, V V Elagin, D S Kuznetsova","doi":"10.17691/stm2024.16.3.04","DOIUrl":"10.17691/stm2024.16.3.04","url":null,"abstract":"<p><p>Presently, there is a need in the developing new approaches to stimulate liver regeneration, which would make its recovery more effective after resection. Application of nanoparticles, loaded with small bioactive molecules, with their targeted delivery into the liver is a promising approach. <b>The aim of the investigation</b> is to study the interaction of nanoparticles with various types of hepatic cells on the models of liver slices and primary hepatic cell cultures using the methods of multiphoton microscopy with fluorescence lifetime imaging.</p><p><strong>Materials and methods: </strong>Nanoparticles have been synthetized from polylactide (PLA), gold (Au), and silicon (SiO₂), and characterized using scanning and transmission electron microscopy. These types of particles were labeled with a fluorescent Cy5 dye for their visualization. Liver slices and a primary hepatocyte culture were used as models for biological testing of nanoparticles. Biodistribution of the nanoparticles in the tissue and cells, their cytotoxicity, and the effect on the cell metabolism were assessed using optical bioimaging methods.</p><p><strong>Results: </strong>The silicon nanoparticles are accumulated mainly by macrophages, which generate reactive oxygen species in a large amount and impair the native metabolic state of hepatocytes. The gold nanoparticles accumulate in all types of the liver cells but possess a marked toxic effect, which is indicated by the appearance of necrotic and apoptotic cells and a sharp change in the hepatocyte metabolic state. The polylactide nanoparticles accumulate most effectively in the liver cells, preferably in hepatocytes, do not change their native metabolic state, making this type of nanoparticles most promising for creating the bioactive molecule delivery systems to stimulate liver regeneration.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 3","pages":"31-41"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11618528/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142804418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peritumoral Brain Zone in Astrocytoma: Morphology, Molecular Aspects, and Clinical Manifestations (Review).","authors":"A S Grishin, K A Achkasova, L S Kukhnina, V A Sharova, M V Ostapyuk, K S Yashin","doi":"10.17691/stm2024.16.2.08","DOIUrl":"10.17691/stm2024.16.2.08","url":null,"abstract":"<p><p>A peritumoral brain zone is an area between a tumor and nontumorous brain tissue with tumor cell infiltration. The identification of this area is sufficiently difficult due to the lack of clear morphological or some other criteria. Besides, its dimensions may vary considerably. In the present review, we have analyzed the available data on the morphological structure and metabolism of peritumoral zone in astrocytomes, and considered the main molecular and genetic aspects and clinical manifestations. Exploration of the peritumoral zone is of great importance for determining the extent of resection to prevent recurrence and to reveal the causes and mechanisms of continued tumor growth.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 2","pages":"79-88"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11556047/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Major Mutations in <i>CFTR, SERPINA1, HFE</i> Genes in Benign Unconjugated Hyperbilirubinemia Phenotype.","authors":"A A Ivanova, N E Apartseva, A P Kashirina, E G Nemtsova, Y V Ivanova, M V Kruchinina, S A Kurilovich, V N Maksimov","doi":"10.17691/stm2024.16.4.04","DOIUrl":"https://doi.org/10.17691/stm2024.16.4.04","url":null,"abstract":"<p><p><b>The aim of the study</b> was to search for the associations of benign unconjugated hyperbilirubinemia phenotype with rs1799945 (H63D), rs1800562 (C282Y), rs1800730 (S65C) mutations of <i>HFE</i> gene, rs113993960 (ΔF508) of <i>CFTR</i> gene, rs28929474 (PIZ), rs17580 (PIS) mutations of <i>SERPINA1</i> gene.</p><p><strong>Material and methods: </strong>The study design is case-control. The group with Gilbert's syndrome (GS) phenotype (n=414; mean age - 36.7±15.9 years; 49.8% men) was formed by gastroenterologists, and included the individuals with unconjugated hyperbilirubinemia who underwent a standard clinical examination. The individuals with known causes of unconjugated hyperbilirubinemia were excluded from the group. The control group (n=429; mean age - 38.5±14.3 years; 52.2% men) was a random sampling from DNA banks of MONICA project participants, the screening of young people aged 25-44 and a one-time study of schoolchildren in Novosibirsk (Russia). DNA was isolated by phenol-chloroform extraction or by the express method (PROBA-RAPID-GENETIKA; DNA-Technology, Moscow, Russia) from venous blood. Genotyping of groups by nucleotide sequence rs1799945 (H63D), rs1800562 (C282Y), rs1800730 (S65C) of <i>HFE</i> gene, rs113993960 (ΔF508) of <i>CFTR</i> gene, rs28929474 (PIZ), rs17580 (PIS) of <i>SERPINA1</i> gene was performed by polymerase chain reaction followed by the analysis of fragment length polymorphism on a polyacrylamide gel.</p><p><strong>Results: </strong>According to the genotypes and alleles of the variants rs1799945 (H63D), rs1800562 (C282Y), rs1800730 (S65C) of <i>HFE</i> gene, rs113993960 (ΔF508) of <i>CFTR</i> gene, rs28929474 (PIZ), rs17580 (PIS) of <i>SERPINA1</i> gene, no statistically significant differences were found between the GS group and the control group (p>0.05).</p><p><strong>Conclusion: </strong>Nucleotide sequence variants rs1799945 (H63D), rs1800562 (C282Y), rs1800730 (S65C) of <i>HFE</i> gene, rs113993960 (ΔF508) of <i>CFTR</i> gene, rs28929474 (PIZ), rs17580 (PIS) of <i>SERPINA1</i> gene, or their combinations with rs3064744 of <i>UGT1A1</i> gene were found to have no association with GS.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 4","pages":"38-44"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11773142/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143070665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experience of Using Neural Networks to Assess Age-Related Changes in Some Structures of the Skull and Cervical Vertebrae Based on CT Scans (Pilot Project).","authors":"G V Zolotenkova, D K Valetov, M P Poletaeva, Yu V Vassilevski","doi":"10.17691/stm2024.16.2.03","DOIUrl":"10.17691/stm2024.16.2.03","url":null,"abstract":"<p><p><b>The aim of the investigation</b> is to study the possibility of using artificial intelligence technologies for age prediction based on CT studies of some structures of the skull and cervical vertebrae.</p><p><strong>Material and methods: </strong>The study included 223 tomograms of the head and neck in sagittal projection from patients without any pathology of the studied structures. Morphometric analysis was carried out using PjaPro and Gradient programs, statistical analysis was performed by SPSS Statistics software. A fully convolutional EfficientNet-B2 neural network was used, which was trained in two stages: selection of the area of interest and solution of regression tasks.</p><p><strong>Results: </strong>Morphometric assessment and subsequent statistical analysis of the selected group of features have shown presence of the strongest correlation with age in the indicator characterizing the involution of the median atlantoaxial joint. A deep learning method using the convolutional network, which automatically selects the desired area in the image (the area of the vertebral junction), classifies the sample, and makes an assumption about the age of the unknown individual with an accuracy of 7.5 to 10.5 years has been tested.</p><p><strong>Conclusion: </strong>As a result of the study, a positive experience has been obtained indicating the possibility of using convolutional neural networks to determine the age of the unknown person, which expands the evidence base and provides new opportunities for determining group-wide personality traits in forensic medicine.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 2","pages":"29-38"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11556052/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-adhesive Properties of Epoxy-Treated Xenopericardium Modified with Polyvinyl Alcohol: <i>in vitro</i> Study of Leukocyte Adhesion in the Pulsatile Flow Model.","authors":"E A Ovcharenko, T V Glushkova, D K Shishkova, M A Rezvova, E A Velikanova, K Yu Klyshnikov, T N Akentyeva, A E Kostyunin","doi":"10.17691/stm2024.16.2.04","DOIUrl":"10.17691/stm2024.16.2.04","url":null,"abstract":"<p><p><b>The aim of the study</b> is to assess protective capabilities of the polymer coating made of polyvinyl alcohol to prevent leukocyte adhesion to epoxy-treated bovine pericardium, which is used in production of bioprosthetic heart valves.</p><p><strong>Materials and methods: </strong>Fragments of unmodified (control) and modified with polyvinyl alcohol epoxy-treated bovine pericardium were incubated in the dedicated chambers connected to a pulsatile flow system (Ibidi GmbH, Germany). During 48 h incubation was conducted in whole donor plasma containing 3<b>·</b>10⁶ of mononuclear fraction cells. To simulate plasma flow, the shear stress on the inflow and outflow sides of of bioprosthetic heart valve in the aortic position was set to 50 and 20 dynes/cm², respectively. After the experiment was completed, the surface of the studied samples was subjected to scanning electron microscopy and immunofluorescence using antibodies to the pan-leukocyte marker CD45.</p><p><strong>Results: </strong>Adhesion of leukocytes (CD45⁺) was seen for both the serous (outflow side) and fibrous (inflow side) surfaces of the control epoxy-treated bovine pericardium, whereas both surfaces of the material modified with polyvinyl alcohol were clear of immune cells. Scanning electron microscopy confirmed the adhesion of leukocytes to intact biological tissue: the cells on the surface of the control xenopericardium were of an irregular shape and formed numerous pseudopodia.</p><p><strong>Conclusion: </strong>The suggested modification of epoxy-treated bovine pericardium with polyvinyl alcohol prevents the adhesion of immune cells to the implant surface and can potentially protect bioprosthetic heart valves from immune rejection.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 2","pages":"40-44"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11556051/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142636182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential of Current Direct Mechanical Testing Methods in Assessing Intraoperative Samples of Aortic Aneurysm Caused by Uncontrolled Arterial Hypertension.","authors":"V N Nikolenko, Y V Belov, M V Oganesyan, Y M Efremov, N A Rizaeva, A D Vovkogon, A V Sankov, L A Gridin, P S Timashev, K V Bulygin, M V Sankova","doi":"10.17691/stm2024.16.4.05","DOIUrl":"https://doi.org/10.17691/stm2024.16.4.05","url":null,"abstract":"<p><p><b>The aim of the study</b> was to investigate the potential of direct mechanical testing methods in clinical practice to assess the strength and elastic-deformative characteristics of intraoperative samples of aortic arch aneurysm caused by uncontrolled arterial hypertension.</p><p><strong>Materials and methods: </strong>The study experimental material was the resected parts of the aortic aneurysm obtained during aneurysm replacement surgery in a patient with uncontrolled arterial hypertension. The direct mechanical testing methods such as instrumental indentation and uniaxial extension were used.</p><p><strong>Results: </strong>It was shown that by the direct instrumental indentation it is possible to accurately assess and compare the stiffness of all three layers of the aortic wall. In this clinical case, the inner aorta layer was subject to the greatest atherosclerotic damage. In the media area, the values of this indicator were widely scattered, whereas the material was greatly dissected. By uniaxial extension method it is possible to obtain accurate parameters of the vascular wall strength, as well as to assess the stiffness, elasticity, and deformability of the intraoperatively resected aortic tissue. It was found that the aneurysm aortic wall, compared with the non-dilated aortic section, was characterized by a significantly lower strength in both longitudinal (by 4.25 times) and transverse (by 3.75 times) directions. In addition, aneurysm tissues demonstrated a significantly lower elasticity and deformability.</p><p><strong>Conclusion: </strong>The study demonstrated the perspectives and options of using in clinical practice current methods of direct mechanical testing, which makes it possible to obtain more accurate indicators of the strength and elastic-deformative vascular characteristics, to clarify the pathophysiological mechanisms of cardiovascular accidents, and to justify the need for regular monitoring of vascular wall stiffness in clinical practice, in particular in patients with uncontrolled arterial hypertension.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 4","pages":"46-53"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11773140/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143070671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Deep Learning Algorithms Based on the Multilayer Y0L0v8 Neural Network to Identify Fungal Keratitis.","authors":"A V Sitnova, E R Valitov, S N Svetozarskiy","doi":"10.17691/stm2024.16.4.01","DOIUrl":"https://doi.org/10.17691/stm2024.16.4.01","url":null,"abstract":"<p><p><b>The aim of the study</b> is to develop a method for diagnosing fungal keratitis based on the analysis of photographs of the anterior segment of the eye using deep learning algorithms with subsequent evaluation of sensitivity and specificity of the method on a test data set in comparison with the results of practicing ophthalmologists.</p><p><strong>Materials and methods: </strong>The study has included the stages of data acquisition, image pre-training and markup, selection of training approach and neural network architecture, training with input data augmentation, validation with hyperparameter correction, evaluation of algorithm performance on a test sample, and determination of sensitivity and specificity of fungal keratitis detection by practicing doctors. A total of 274 anterior segment images were used, including 130 photographs of the eyes affected by fungal keratitis and 144 photographs illustrating normal eyes, keratitis of other etiologies, and various anterior segment pathologies. Photographs taken after the treatment onset, illustrations of keratitis of mixed etiology and corneal perforation were excluded from the study. Images of the training sample were marked up using the VGG Image Annotator web application and then used to train the YOLOv8 convolutional neural network. Images from the test data set were also offered to practicing ophthalmologists to determine the diagnostic accuracy of fungal keratitis.</p><p><strong>Results: </strong>The sensitivity of the model was 56.0%, the specificity level reached 96.1%, and the proportion of correct answers of the algorithm was 76.5%. The accuracy of image recognition by practicing ophthalmologists was 50.0%, specificity - 41.7%, sensitivity - 57.7%.</p><p><strong>Conclusion: </strong>The study showed the high potential of deep learning algorithms in the diagnosis of fungal keratitis and its advantages in accuracy compared to expert judgment in the absence of metadata. The use of computer vision technologies may find application as a complementary diagnostic method in decision making in complex cases and in telemedicine care settings. Further research is required to compare the developed model with alternative approaches, to expand and standardize databases.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 4","pages":"5-13"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11773139/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143070657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Skin Recovery after Fractional CO₂ Laser Treatment Lichen Sclerosus Using Multimodal Optical Coherence Tomography.","authors":"M A Sirotkina, A L Potapov, M M Loginova, A E Bychkova, A A Moiseev, M V Kochuyeva, A Yu Bogomolova, E B Kiseleva, A V Asaturova, A V Maslennikova, S G Radenska-Lopovok, L A Apolikhina, N D Gladkova","doi":"10.17691/stm2024.16.4.02","DOIUrl":"https://doi.org/10.17691/stm2024.16.4.02","url":null,"abstract":"<p><p><b>The aim of the study</b> is to demonstrate the effect of fractional CO₂ laser treatment on vulvar skin in lichen sclerosus using multimodal optical coherence tomography (MM OCT).</p><p><strong>Materials and methods: </strong>The study included 3 clinical cases of vulvar lichen sclerosus (VLS) (histologically classified as early, early with dermal edema, late) and 2 control cases without vulvar pathology. Patients with VLS underwent 3 procedures of fractional CO₂ laser treatment with an interval of 30-40 days. In patients without vulvar pathology, the MM OCT examination was performed once, in patients with VLS it was done at the point of the greatest visible skin damage just before a punch biopsy, immediately after the first laser session, and 1 month after a full course of treatment.</p><p><strong>Results: </strong>Analyzing the case series of vulvar skin recovery 1 month after fractional CO₂ laser treatment, it has been found that the recovery depended on the dermal condition before the treatment. In early VLS and early VLS with dermal edema (clinical cases 1 and 2) before the treatment, the MM OCT examination has shown a decreased epidermal thickness, unclear contrast between the epidermis and dermis, a decrease in the values of the OCT signal attenuation coefficient in the dermis, and a sharp reduction in the density of blood and lymphatic vessels. After treatment, the MM OCT examination demonstrated a complete recovery of vulvar skin structure and all quantitative values reached the level characteristic of normal vulvar skin. Before the treatment, a homogenization zone (sclerosis) was clearly visible in the MM OCT images of the dermis in late VLS (clinical case 3). After the treatment, complete recovery of the vulvar skin structure was not observed; the area of sclerosis was preserved; however, visually, there was an increase in the density of blood and lymphatic vessels in the affected area, which almost reached the level of normal vulvar skin.</p><p><strong>Conclusion: </strong>MM OCT can be proposed as a promising non-invasive method for monitoring skin recovery after fractional CO₂ laser treatment of VLS.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 4","pages":"15-26"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11773141/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143070670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}