Sovremennye tekhnologii v meditsine最新文献

{"title":"Development of DNA Microarray for Indication of Viral Community-Acquired Pneumonia Pathogens.","authors":"N A Sakharnov, E N Filatova, M I Popkova, L B Lukovnikova, M O Bahmeteva, S L Slavin, O V Utkin","doi":"10.17691/stm2025.17.3.02","DOIUrl":"10.17691/stm2025.17.3.02","url":null,"abstract":"<p><p><b>The aim of the study</b> is to develop a DNA microarray for the indication of viral pathogens causing community-acquired pneumonia.</p><p><strong>Materials and methods: </strong>The study materials were swab samples from the nasopharyngeal and oropharyngeal mucous membranes of patients aged 2 months to 18 years with X-ray confirmed pneumonia. The selection of DNA probes for the specific detection of viral community-acquired pneumonia pathogens and development of the microarray design were carried out using our previously developed disprose program. The nucleotide sequences of pathogens were obtained from the NCBI Nucleotide and GISAID databases. The selected DNA probes were synthesized on CustomArray slides (USA). The optimal hybridization temperature was selected on a model pooled sample containing adenovirus DNA and SARS-CoV-2 coronavirus RNA. The selection criteria were the percentage of effective probes with a standardized hybridization signal (SHS) ≥3 Z and the excess of SHS levels of effective specific probes compared to SHS of effective non-specific probes. The DNA probes were selected for the specific detection of viral community-acquired pneumonia pathogens, characterized by an effective hybridization signal under the identified conditions. Using ROC analysis, threshold values of specific probe signals were established, the excess of which was interpreted as the evidence of the pathogen presence in a sample.</p><p><strong>Results: </strong>A microarray design included 544 DNA probes for the detection of adenovirus, bocavirus, respiratory syncytial virus, metapneumovirus, parainfluenza virus, rhinovirus, and coronavirus. The DNA probes were synthesized on slides. The optimal DNA hybridization temperature on microarrays was established (47°C). A list of probes for specific detection of adenovirus group B, bocavirus, parainfluenza virus type 3, respiratory syncytial virus, rhinovirus, and SARS-CoV-2 coronavirus, characterized by an effective hybridization signal under the identified conditions, was selected. The threshold values of probe signals for specific detection of these pathogens in clinical samples were determined.</p><p><strong>Conclusion: </strong>A DNA microarray for the indication of viral community-acquired pneumonia pathogens was developed and synthesized. The interpretation of the hybridization results corresponds to the results obtained by the PCR method. The developed microarray can be used to improve laboratory diagnostics of viral community-acquired pneumonia pathogens.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"17 3","pages":"17-28"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12261293/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144651964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of Macrophages in Implementing the Effects of Secretome of Mesenchymal Stromal Cells in the Spermatogonial Stem Cell Niche.","authors":"A O Monakova, N A Basalova, V Yu Balabanyan, K L Kryshen, A A Matichin, G D Sagaradze, V S Popov, A Yu Efimenko","doi":"10.17691/stm2025.17.2.04","DOIUrl":"10.17691/stm2025.17.2.04","url":null,"abstract":"<p><p>Postnatal stem cells surrounded by a niche support the renewal and regeneration of tissues and organs throughout life. The dysfunction of niche components can lead to the development of diseases, which are hard to cure. We previously showed that the subtunical injection of mesenchymal stromal cell (MSC) secretome induced the spermatogonial stem cell niche restoration, although the mechanisms of the process are not fully revealed. <b>The study aimed</b> at analyzing the effect of the MSC secretome on resident macrophages in animal models of male infertility and on peritoneal macrophages of intact animals.</p><p><strong>Materials and methods: </strong>To study the effect of the MSC secretome on resident macrophages, doxorubicin-induced damage of murine spermatogenesis was modeled by intraperitoneal injections of 1 mg/kg of doxorubicin once in two days to reach a cumulative dose of 10 mg/kg. The second animal model of spermatogenesis injury was the abdominal cryptorchidism in rats. The MSC secretome was injected under the tunica albuginea. The animals were divided into the following groups: \"intact\", \"damage\", \"MSC secretome\". After isolating the testes, the number of macrophages was estimated using the immunohistochemical analysis. To investigate the phagocytic activity of macrophages mice were intramuscularly injected into the thigh with the MSC secretome with the following isolation of peritoneal macrophages. The ability of peritoneal macrophages to absorb FITC latex particles was analyzed.</p><p><strong>Results: </strong>In cryptorchidism model the number of CD163⁺ M2 macrophages in the interstitium of testes increased significantly. The MSC secretome injection under the tunica albuginea of the testicle led to decreasing the number of CD163⁺ M2 macrophages. In the model of the toxic damage of spermatogenesis with doxorubicin the number of CD163⁺ M2 macrophages in the interstitium increased, however, there were no effects in the group with the MSC secretome injection. The number of M2 macrophages in this model, positive for another classical marker CD206, also increased, but the administration of MSC secretome reduced their number neither during the early nor in the late periods after damage. The study of MSC secretome effects on peritoneal macrophages demonstrated that a single intramuscular injection of MSC secretome in doses lower and higher than the therapeutic dose didn't reduce, but conversely increased the phagocytic activity of macrophages.</p><p><strong>Conclusion: </strong>Our findings indicate the impact of the damage etiology and pathogenesis on the involvement of M2 macrophages in the implementation of the MSC secretome effects and the absence of its systemic immunotoxicity.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"17 2","pages":"37-45"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12096361/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144145144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Redox-Sensitive Fluorescent Nanoparticles for Biovisualization of Malignant Tumors.","authors":"O Peltek, E A Kopoleva, M V Zyuzin","doi":"10.17691/stm2025.17.1.05","DOIUrl":"10.17691/stm2025.17.1.05","url":null,"abstract":"<p><p>Application of fluorescent redox-sensitive nanoparticles in current biomedicine ensures high sensitivity and accuracy of biovisualization. Nanoparticles are potent as they can long circulate in the blood, where the level of glutathione is relatively low, and are destroyed in tumor cells, releasing loaded dyes or drugs. <b>The aim of the study</b> was to develop new nanoparticles based on trithiocyanuric acid for biovisualization of malignant tumors and study capabilities of the developed nanoparticles.</p><p><strong>Materials and methods: </strong>Nanoparticles were obtained by polycondensation of trithiocyanuric acid using iodine. Scanning and transmission electron microscopy was used for their characterization, the loading of fluorescent dyes was assessed by means of spectrophotometry. Confocal laser scanning microscopy was applied to study the impact of nanoparticles on the viability of the 4T1 and A549 cell lines as well as their interaction with cells. The distribution of nanoparticles in tissues and organs of BALB/c model mice with grafted tumors was performed using fluorescence visualization.</p><p><strong>Results: </strong>According to scanning microscopy, the size of the synthesized particles reached 100±20 nm. The adsorption isotherm demonstrated that adsorption of 0.27 mg of the RhB fluorescent dye per 1 mg of nanoparticles could be achieved. Enhanced release of the packed fluorescent dye was seen in the presence of glutathione and acetylcysteine. The particles did not significantly affect the viability of 4T1 and A549 cells. After intratumoral administration, they ensured a more intense fluorescent signal in the tumor area compared to a regular fluorescent dye solution.</p><p><strong>Conclusion: </strong>The developed system of trithiocyanuric-acid-based nanoparticles demonstrated high efficiency in biovisualization of malignant tumors and has a potential for targeted delivery of treatment agents.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"17 1","pages":"50-56"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11892567/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143607753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impact of Silicon Nanoparticle Porosity on Their Ability to Sensitize Low-Intensity Medical Ultrasound.","authors":"L A Osminkina, P A Tyurin-Kuzmin, M V Sumarokova, A A Kudryavtsev","doi":"10.17691/stm2025.17.1.04","DOIUrl":"10.17691/stm2025.17.1.04","url":null,"abstract":"<p><p>This study investigates the role of porosity in silicon nanoparticles' ability to act as sonosensitizers for sonodynamic therapy of malignant tumors. Structural analysis showed that porous nanoparticles are composed of nanocrystals approximately 4 nm in size and contain 15 nm pores, whereas non-porous nanoparticles have a dense structure with nanocrystals ranging from 10 to 50 nm. Porous nanoparticles exhibit pronounced photoluminescent properties, associated with quantum confinement effects in their small nanocrystals. The cytotoxicity of the nanoparticles was investigated in vitro using Hep2 cells. The results showed that both porous and non-porous nanoparticles in the studied concentration range (2-500 μg/ml) are non-toxic. Low-intensity ultrasound (0.88 MHz, <1 W) also does not have a toxic effect on the cells. However, the combined use of porous nanoparticles and ultrasound led to a significant decrease in cell viability, which was not observed when non-porous nanoparticles were used. This effect is associated with mechanical destruction of the cell membranes, as well as the potential activation of additional cell death mechanisms, such as apoptosis. The results highlight the importance of porosity as a key factor determining the effectiveness of silicon nanoparticles as sonosensitizers. The high efficiency, low toxicity, and unique structural properties of porous nanoparticles make them a promising material for further research and development of targeted, non-invasive treatments for malignant tumors in the context of sonodynamic therapy.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"17 1","pages":"40-47"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11892568/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143607760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Potential of Near-Infrared Spectroscopy as a Therapeutic Tool Following a Stroke (Review).","authors":"O A Mokienko","doi":"10.17691/stm2025.17.2.07","DOIUrl":"10.17691/stm2025.17.2.07","url":null,"abstract":"<p><p>The advancement of novel technologies for the rehabilitation of post-stroke patients represents a significant challenge for a range of interdisciplinary fields. Near-infrared spectroscopy (NIRS) is an optical neuroimaging technique based on recording local hemodynamic changes at the cerebral cortex level. The technology is typically employed in post-stroke patients for diagnostic purposes, including the assessment of neuroplastic processes accompanying therapy, the study of hemispheric asymmetry, and the examination of functional brain networks. However, functional NIRS can also be used for therapeutic purposes, including the provision of biofeedback during rehabilitation tasks, as well as the navigation method during transcranial stimulation. The effectiveness of therapeutic NIRS application in stroke patients remains insufficiently studied, despite existing scientific evidence confirming its promising potential as a treatment method. The review examines the published literature on the therapeutic applications of NIRS after stroke, evaluating its potential role in the rehabilitation process. The paper describes NIRS features, advantages, and disadvantages, determining its position among other neuroimaging technologies; analyzes the findings of neurophysiological studies, which justified the clinical trials of NIRS technology; and evaluates the results of the studies on the therapeutic use of NIRS in post-stroke patients. Two potential applications of NIRS for therapeutic purposes following a stroke were suggested: the first was to provide real-time feedback during movement training (motor or ideomotor ones, including that in brain-computer interface circuits), and the second was to facilitate navigation during transcranial stimulation. Based on a comprehensive literature review, there were proposed and justified further research lines and development in this field.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"17 2","pages":"73-83"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12096357/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144145142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Candidate Genetic Markers of Schizophrenia Based on Exome Sequencing Data and Its Relation to Immunological, Clinical, and Morphometric Changes in the Russian Population.","authors":"S M Rastorguev, S V Tsygankova, M A Kaidan, V L Ushakov, I K Malashenkova, S A Krynskiy, D P Ogurtsov, N A Hailov, N V Zakharova, G P Kostyuk","doi":"10.17691/stm2025.17.2.06","DOIUrl":"10.17691/stm2025.17.2.06","url":null,"abstract":"<p><p><b>The aim of the study</b> was to identify genetic markers of schizophrenia based on exome sequencing data, as well as to specify its potential relation to clinical manifestations of the disease, morphological changes in the brain and immune disorders.</p><p><strong>Materials and methods: </strong>The analyzed sample consisted of 48 patients (23 men and 25 women; average age - 31.5±7.7 years) having a confirmed diagnosis of paranoid schizophrenia.</p><p><strong>Results: </strong>140 genes with differential polymorphisms and enriched categories that may be related to the pathogenesis of schizophrenia were identified. Analysis of genes with differential frequencies of functionally significant common single nucleotide polymorphisms (SNPs) by their major functions showed that the most common were genes involved in regulation of immune system functions and development of the nervous system, as well as genes being structural components of neurons and glia involved in the perception of sensory stimuli. The findings confirm the complexity of the genetic basis of schizophrenia. Analysis of the top 10 genes containing the most differential polymorphisms specifies such genes related to schizophrenia as <i>MUC12</i> and <i>SH3KBP1</i>. The genes involved in regulation of the immune response include <i>HLA-DQB2</i> which is one of the most significantly different SNPs between the group of patients and the general population; <i>HLA-DQB2</i> SNP (rs9276572) in patients is related to the signs of dysfunction of the antiviral component of immune system, structural changes in the brain and cognitive challenges. Although most of the detected genes are unique to the sample studied, additional studies are required to confirm these genes' involvement in the pathogenesis of this disease as well as to identify the mechanisms of the disease onset and development. The rs9276572(C) polymorphism of <i>HLA-DQB2</i> requires further study as a new potential marker of immunological disorders, morphometric changes in the brain and cognitive impairment in schizophrenia. The data obtained indicate the need for personalized medicine, because the majority of genetic prerequisites are patient-specific and highlight the importance of further research to understand the genetic aspects of schizophrenia and develop innovative approaches to its diagnosis and treatment.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"17 2","pages":"55-70"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12096358/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144145207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Major Mutations in <i>CFTR, SERPINA1, HFE</i> Genes in Benign Unconjugated Hyperbilirubinemia Phenotype.","authors":"A A Ivanova, N E Apartseva, A P Kashirina, E G Nemtsova, Y V Ivanova, M V Kruchinina, S A Kurilovich, V N Maksimov","doi":"10.17691/stm2024.16.4.04","DOIUrl":"https://doi.org/10.17691/stm2024.16.4.04","url":null,"abstract":"<p><p><b>The aim of the study</b> was to search for the associations of benign unconjugated hyperbilirubinemia phenotype with rs1799945 (H63D), rs1800562 (C282Y), rs1800730 (S65C) mutations of <i>HFE</i> gene, rs113993960 (ΔF508) of <i>CFTR</i> gene, rs28929474 (PIZ), rs17580 (PIS) mutations of <i>SERPINA1</i> gene.</p><p><strong>Material and methods: </strong>The study design is case-control. The group with Gilbert's syndrome (GS) phenotype (n=414; mean age - 36.7±15.9 years; 49.8% men) was formed by gastroenterologists, and included the individuals with unconjugated hyperbilirubinemia who underwent a standard clinical examination. The individuals with known causes of unconjugated hyperbilirubinemia were excluded from the group. The control group (n=429; mean age - 38.5±14.3 years; 52.2% men) was a random sampling from DNA banks of MONICA project participants, the screening of young people aged 25-44 and a one-time study of schoolchildren in Novosibirsk (Russia). DNA was isolated by phenol-chloroform extraction or by the express method (PROBA-RAPID-GENETIKA; DNA-Technology, Moscow, Russia) from venous blood. Genotyping of groups by nucleotide sequence rs1799945 (H63D), rs1800562 (C282Y), rs1800730 (S65C) of <i>HFE</i> gene, rs113993960 (ΔF508) of <i>CFTR</i> gene, rs28929474 (PIZ), rs17580 (PIS) of <i>SERPINA1</i> gene was performed by polymerase chain reaction followed by the analysis of fragment length polymorphism on a polyacrylamide gel.</p><p><strong>Results: </strong>According to the genotypes and alleles of the variants rs1799945 (H63D), rs1800562 (C282Y), rs1800730 (S65C) of <i>HFE</i> gene, rs113993960 (ΔF508) of <i>CFTR</i> gene, rs28929474 (PIZ), rs17580 (PIS) of <i>SERPINA1</i> gene, no statistically significant differences were found between the GS group and the control group (p>0.05).</p><p><strong>Conclusion: </strong>Nucleotide sequence variants rs1799945 (H63D), rs1800562 (C282Y), rs1800730 (S65C) of <i>HFE</i> gene, rs113993960 (ΔF508) of <i>CFTR</i> gene, rs28929474 (PIZ), rs17580 (PIS) of <i>SERPINA1</i> gene, or their combinations with rs3064744 of <i>UGT1A1</i> gene were found to have no association with GS.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 4","pages":"38-44"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11773142/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143070665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoparticles for Creating a Strategy to Stimulate Liver Regeneration.","authors":"S A Rodimova, D S Kozlov, D P Krylov, L V Mikhailova, V A Kozlova, A I Gavrina, A M Mozherov, V V Elagin, D S Kuznetsova","doi":"10.17691/stm2024.16.3.04","DOIUrl":"10.17691/stm2024.16.3.04","url":null,"abstract":"<p><p>Presently, there is a need in the developing new approaches to stimulate liver regeneration, which would make its recovery more effective after resection. Application of nanoparticles, loaded with small bioactive molecules, with their targeted delivery into the liver is a promising approach. <b>The aim of the investigation</b> is to study the interaction of nanoparticles with various types of hepatic cells on the models of liver slices and primary hepatic cell cultures using the methods of multiphoton microscopy with fluorescence lifetime imaging.</p><p><strong>Materials and methods: </strong>Nanoparticles have been synthetized from polylactide (PLA), gold (Au), and silicon (SiO₂), and characterized using scanning and transmission electron microscopy. These types of particles were labeled with a fluorescent Cy5 dye for their visualization. Liver slices and a primary hepatocyte culture were used as models for biological testing of nanoparticles. Biodistribution of the nanoparticles in the tissue and cells, their cytotoxicity, and the effect on the cell metabolism were assessed using optical bioimaging methods.</p><p><strong>Results: </strong>The silicon nanoparticles are accumulated mainly by macrophages, which generate reactive oxygen species in a large amount and impair the native metabolic state of hepatocytes. The gold nanoparticles accumulate in all types of the liver cells but possess a marked toxic effect, which is indicated by the appearance of necrotic and apoptotic cells and a sharp change in the hepatocyte metabolic state. The polylactide nanoparticles accumulate most effectively in the liver cells, preferably in hepatocytes, do not change their native metabolic state, making this type of nanoparticles most promising for creating the bioactive molecule delivery systems to stimulate liver regeneration.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 3","pages":"31-41"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11618528/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142804418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peritumoral Brain Zone in Astrocytoma: Morphology, Molecular Aspects, and Clinical Manifestations (Review).","authors":"A S Grishin, K A Achkasova, L S Kukhnina, V A Sharova, M V Ostapyuk, K S Yashin","doi":"10.17691/stm2024.16.2.08","DOIUrl":"10.17691/stm2024.16.2.08","url":null,"abstract":"<p><p>A peritumoral brain zone is an area between a tumor and nontumorous brain tissue with tumor cell infiltration. The identification of this area is sufficiently difficult due to the lack of clear morphological or some other criteria. Besides, its dimensions may vary considerably. In the present review, we have analyzed the available data on the morphological structure and metabolism of peritumoral zone in astrocytomes, and considered the main molecular and genetic aspects and clinical manifestations. Exploration of the peritumoral zone is of great importance for determining the extent of resection to prevent recurrence and to reveal the causes and mechanisms of continued tumor growth.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 2","pages":"79-88"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11556047/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A New Neuroanesthetic Protocol of Rendering Specialized Care in Treating Degenerative Lumbar Spine Diseases in High-Risk Patients: Prospective Analysis of the Results.","authors":"A A Kalinin, V Yu Goloborodko, Yu Ya Pestryakov, R A Kundubayev, M Yu Biryuchkov, A V Shchegolev, V A Byvaltsev","doi":"10.17691/stm2024.16.3.06","DOIUrl":"10.17691/stm2024.16.3.06","url":null,"abstract":"<p><p><b>The aim of the study</b> is to assess the effectiveness of a new neuroanesthetic protocol for treating degenerative lumbar spine diseases in high-risk patients.</p><p><strong>Materials and methods: </strong>Two groups of patients with a high risk of anesthesia and surgery determined by the authors' clinical decision support system (CDSS) have been prospectively studied. A new neuroanesthetic protocol was used in the experimental group (EG, n=25), while the control group (CG, n=25) underwent intravenous anesthesia based on propofol and fentanyl. Minimally invasive transforaminal lumbar interbody fusion was performed in all cases. Changes of the intraoperative mean arterial pressure and heart rate, intensity of the local pain syndrome, amount of the opiates used, presence of cognitive disorders, adverse effects of anesthesia, and surgical complications have been compared.</p><p><strong>Results: </strong>The groups were representative (p>0.05) in terms of the age-gender parameters, anthropological data, comorbid background, involvement in smoking, preoperative characteristics of the lumbar spine, as well as the level of cognitive functions. No statistically significant changes of the mean arterial pressure (p=0.17) were registered in EG patients relative to the CG (p=0.0008). Intraoperative reduction of the heart rate in patients of the CG was not noted (p=0.49) in comparison with the EG (p=0.03). In the postoperative period, the best indicators of cognitive functions on the FAB test (p=0.02) and MoCA test (p=0.03) were revealed in EG. A significantly less amount of perioperative opiates (p=0.005) at a low level of the local pain syndrome was also noted (p=0.01). The intergroup analysis has shown fewer adverse effects of anesthesia in EG compared to CG (p=0.01) with a comparable number of postoperative surgical complications (p=0.42).</p><p><strong>Conclusion: </strong>A new neuroanesthetic protocol of rendering a specialized care to patients with a high risk of anesthesia and surgery, assessed by the authors-developed CDSS, has resulted in effective elimination of the local postoperative pain syndrome, reduction of perioperative application of opioids, and stabilization of intraoperative indicators of cardiovascular activity. In addition, no postoperative cognitive disorders, anesthetic side-effects, adverse pharmacological consequences of the complex usage of non-steroidal anti-inflammatory drugs, prolonged local anesthetics, alpha-2-agonist, and non-narcotic analgesics have been registered.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 3","pages":"51-59"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11618530/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142804410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}