Sovremennye tekhnologii v meditsine最新文献

{"title":"Diagnosis of Colorectal Cancer and Adenomatous Polyps of the Colon Based on the Level of MicroRNA Expression in the Mucous Membrane (Pilot Clinical Study).","authors":"M V Bagryantsev, A A Yanyshev, M G Ryabkov, A I Abelevich, I L Dezortsev, A V Bazayev","doi":"10.17691/stm2024.16.5.05","DOIUrl":"10.17691/stm2024.16.5.05","url":null,"abstract":"<p><p>The gold standard for colorectal cancer (CRC) diagnosis, colonoscopy with biopsy, is an invasive technique and has some limitations, while the known non-invasive methods do not possess sufficient sensitivity and specificity. The application of microRNA as a diagnostic and prognostic CRC biomarker may compensate for the colonoscopy limitations. However, there are no data in the literature on the existence of real test systems based on the evaluation of microRNA expression. Our pilot study is the first step to creating a test system for CRC diagnosis based on the analysis of microRNA expression in the tissue of the intact colon. <b>The aim of the study</b> is to assess the prospects of using the level of microRNA expression as a supplemental method for diagnosing colorectal cancer and adenomatous polyps.</p><p><strong>Materials and methods: </strong>Patients participating in the study were divided into three groups: group 1 included patients with CRC (n=5), group 2 - patients with polyps in the colon (n=4), and patients without oncological pathology treated for hemorrhoidal disease without exacerbation (n=5) composed group 3.Tissue samples of the intact gut were taken from all patients. In groups 1 and 2, biopsy was performed in the process of right-sided laparoscopic resection of the colon with tumor. Samples of the mucous membrane from the distal part of the rectum in patients of group 3 were also collected intraoperatively; they were operated on using Milligan-Morgan technique. In groups 1 and 2, CRC and polyp samples, respectively, were taken for the analysis additionally to the intact gut area.The test panel included the following microRNAs: hsa-miR-10b-5p, hsa-miR-20a-5p, hsa-miR-141-3p, hsa-miR-181b-5p. The levels of the reference genes were analyzed with the help of real-time polymerase chain reaction using intercalating SYBR Green stain.</p><p><strong>Results: </strong>Expression of hsa-miR-141-3p in the mucous membrane of the colon in patients of groups 1 and 2 (with CRC and polyps, respectively) was statistically significantly higher than in patients without bowel tumors. At the same time, the expression level of hsa-miR-10b-5p was statistically significantly lower in the tumor tissue (cancer or polyps) in comparison with patients of group 3.Lower values of expression in all tested microRNAs have been detected in the CRC tissue relative to the intact mucosa of the same patients. A similar tendency was also observed in patients with adenomatous polyps.</p><p><strong>Conclusion: </strong>The results of the study have shown that of four microRNAs, included into the test panel, hsa-miR-141-3p and hsa-miR-10b-5p were found to have the diagnostic value for identifying tumor colorectal lesions. Thus, our data will assume that supplementing the endoscopic tests of the large intestine by the epigenetic analysis of the mucous membrane is a promising approach to cancer screening procedures.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 5","pages":"45-51"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11784883/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143083103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Poly-3-Hydroxybutyrate Enriched with Simvastatin in Bone Regeneration after Tooth Extraction (Experimental Study).","authors":"K M Salekh, A A Muraev, A A Dolgalev, A B Dymnikov, G A Bonartseva, T K Makhina, D V Chesnokova, V V Voinova, A P Bonartsev, M E Mokrenko, S Y Ivanov","doi":"10.17691/stm2024.16.5.03","DOIUrl":"10.17691/stm2024.16.5.03","url":null,"abstract":"<p><p>Physiological resorption of bone tissue after tooth extraction leads to a decrease in the volume of bone tissue available for implantation and makes it difficult to install dental implants. Preservation of the well after tooth extraction is the solution to this problem, with the choice of bone plastic material playing an important role. The development of an \"ideal\" bone plastic material with osteoinductive properties that promotes reparative bone regeneration remains an urgent task. <b>The aim of the study</b> was to evaluate the regeneration of bone tissue of the alveolar ridge during implantation of a new osteoinductive bone plastic material containing simvastatin into the wells of extracted teeth in sheep using microcomputer tomography.</p><p><strong>Materials and methods: </strong>The study was conducted on 24 adult sheep with a total of 48 teeth removed. 12 wells were filled with material based on poly(3-hydroxybutyrate) (PHB) with simvastatin; 12 wells were filled with PHB-based material without simvastatin, 24 wells were used as a control. Micro-CT was used for comparative analysis of bone tissue formation between the test groups after 3 and 6 months.</p><p><strong>Results: </strong>The results of the study confirm the positive effect of simvastatin released from the PHB-based osteoplastic material on the volume of the formed bone tissue and the total bone volume in the defect area (BV/TV) and bone mineral density (BMD) 3 and 6 months after surgery.</p><p><strong>Conclusion: </strong>The study demonstrated that simvastatin, released from the PHB-based osteoplastic material, has an osteoinductive effect, promoting bone tissue regeneration in the wells left after tooth removal. Higher BV/TV and BMD values in the wells indicate better efficacy of the material in terms of regeneration support.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 5","pages":"27-33"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11784884/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143083070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Risk Factors for Nontuberculous Mycobacterial Pulmonary Disease (Systematic Review).","authors":"P S Sviridov, M M Litvinova, M A Karnaushkina, N N Makaryants, M V Gorbunova","doi":"10.17691/stm2024.16.5.07","DOIUrl":"10.17691/stm2024.16.5.07","url":null,"abstract":"<p><p>This paper is a systematic review of the published data describing genetic risk factors for pulmonary diseases caused by nontuberculous mycobacteria (nontuberculous mycobacterial pulmonary disease - NTM-PD). <b>The aim of the study</b> is to compile a specific list of genetic markers associated with the risk of developing NTM-PD. This literature review was prepared according to PRISMA criteria and was registered in the International Prospective Register of Systematic Reviews (PROSPERO) (registration number CRD42019128569). In the process of work, a great number of articles from PubMed, Google Scholar, and ScienceDirect databases have been studied. Using careful analysis and selection procedures, a list of 14 genetic variants associated with an increased risk of developing NTM-PD was generated. <i>SLC11A1, NLRP3, TLR2, CFTR, IFNGR1, PDCD1</i> genes have been found to refer to these variants as well as variants in the intergenic regions affecting expression of <i>STK17A, IFNL3, TNF, IL10</i> genes. The products of these genes take different roles in regulating the response to various pathogenic factors, and some of them are poorly understood. For a more precise and detailed explanation of the influence of these genetic variants, further studies in patient groups of different populations with the evaluation of different combinations of variants and intergenic interaction are required.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 5","pages":"62-71"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11784881/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143083085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Technology of Combined Identification of Macrophages and Collagen Fibers in Liver Samples.","authors":"I A Nikitina, V A Razenkova, E A Fedorova, O V Kirik, D E Korzhevskii","doi":"10.17691/stm2024.16.3.03","DOIUrl":"10.17691/stm2024.16.3.03","url":null,"abstract":"<p><p>The importance of identifying pathological changes in the liver both in fundamental researches and in diagnostic practice dictates the necessity to have a convenient method of assessing functional condition of resident macrophages and connective tissue fibers. <b>The aim of the study</b> is to assess the technology of combined histo-immunohistochemical detection of collagen fibers of connective tissue and resident liver macrophages using aniline blue histological stain and available antibodies to the microglial marker, the Iba-1 protein.</p><p><strong>Materials and methods: </strong>Liver samples from adult rats (n=6) have been used in the study. The connective tissue was stained with a 2% aqueous solution of aniline blue (Unisource Chemicals Ltd., India). Monoclonal rabbit antibodies to Iba-1 (Clone JM36-62; ET1705-78; HuaBio, China) were used to detect resident liver macrophages, zinc-ethanol-formaldehyde was employed as a fixative.</p><p><strong>Results: </strong>The combined staining method allowed us to detect numerous Iba-1-immunopositive structures corresponding morphologically to Kupffer cells and connective tissue macrophages, background staining was not observed. Staining with aniline blue in the liver samples was selective, uniform, and clear, and allowed for differentiation of the connective tissue in all examined samples. Exclusion of the heat-induced epitope retrieval stage caused no negative effect on identification of macrophages, reduced the probability of non-specific staining of the collagen fibers with aniline blue, and ensured general preservation of tinctorial properties of the liver tissue.</p><p><strong>Conclusion: </strong>The presented protocol of combined histo-immunohistochemical identification of Kupffer cells and connective tissue fibers, applied on the rat liver samples, makes it possible to perform effectively the morphometric analysis and may find its application in pathohistological, clinical, and preclinical investigations.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 3","pages":"24-29"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11618527/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142804420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effects of Mucolytic Agents N-Acetylcysteine and Erdosteine on Hemostasis in Humans.","authors":"S Oktar, S Doğru, S Motor, M Demirköse, E Ş Erden","doi":"10.17691/stm2024.16.4.06","DOIUrl":"https://doi.org/10.17691/stm2024.16.4.06","url":null,"abstract":"<p><p>There are some indications in a few studies on animals and humans that the mucolytic agents N-acetylcysteine and erdosteine have a disruptive effect on the coagulation cascade. This study <b>aimed</b> to examine the effects of both erdosteine and N-acetylcysteine on the coagulation pathway.</p><p><strong>Materials and methods: </strong>The research was conducted on patients treated with N-acetylcysteine (600 mg/day) or erdosteine (600 mg/day) for 7-14 days. To evaluate the coagulation and hemogram parameters, blood samples were taken from patients before treatment and on days 7-14 after starting the treatment. Hemogram and coagulation parameters were measured in the venous blood samples.</p><p><strong>Results: </strong>The levels of factor II significantly decreased, and the platelet count significantly increased in patients treated with erdosteine. D-dimer levels reduced and factor VII levels increased in patients treated with N-acetylcysteine, but these changes were not significant (p=0.069 and p=0.062, respectively). Other coagulation and hemogram values did not change in both groups. Erdosteine may have a dualistic effect on the coagulation cascade through its different metabolites.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 4","pages":"55-60"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11773143/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143070673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prediction of Hospital Mortality in Patients with ST Segment Elevation Myocardial Infarction: Evolution of Risk Measurement Techniques and Assessment of Their Effectiveness (Review).","authors":"B I Geltser, I G Domzhalov, K I Shakhgeldyan, N S Kuksin, E A Kokarev, R L Pak, V N Kotelnikov","doi":"10.17691/stm2024.16.4.07","DOIUrl":"https://doi.org/10.17691/stm2024.16.4.07","url":null,"abstract":"<p><p>Risk stratification of hospital mortality in patients with ST segment elevation myocardial infarction on the electrocardiogram is an important part of the specialized medical care provision. The systematic review presents scientific literature data characterizing the predictive value of both classical prognostic scales (GRACE, CADDILLAC, TIMI risk score for STEMI, RECORD, etc.) and new risk measurement tools developed on the basis of modern machine learning techniques. Most studies on this issue are often focused on the search for new predictors of adverse events, which allow to detail the relations between indicators of the clinical and functional status of patients and the end point of the study. Here, an important task is to develop hospital mortality prognostic algorithms characterized by explainable artificial intelligence and trusted by doctors.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 4","pages":"61-72"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11773138/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143070672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of a Body Shape Index as an Anthropometric Predictor of Cardiometabolic Risks in Children and Adolescents (Systematic Review).","authors":"S A Todorova","doi":"10.17691/stm2024.16.5.06","DOIUrl":"10.17691/stm2024.16.5.06","url":null,"abstract":"<p><p>A body shape index (ABSI) is a novel anthropometric measure associated with body obesity. However, the research on the relationship between the new anthropometric risk index and metabolic disorders and cardiovascular alterations in the pediatric population is limited. <b>The aim of the study</b> was to perform a systematic review and meta-analysis of the associations between cardiometabolic risk and ABSI and evaluate its discrimination capacity.</p><p><strong>Materials and methods: </strong>A systematic search and analysis of the available scientific literature were conducted to identify relevant articles published up to March 2024 in Web of Science, Science Direct, Scopus, ResearchGate, and PubMed. All full-text publications reporting an association between cardiometabolic risk and ABSI in children and adolescents were considered eligible and reviewed in detail. The reported results and statements were summarized using meta-analysis methods.</p><p><strong>Results: </strong>A systematic review of 17 cross-sectional studies involving 31,849 children and adolescents, predominantly from Asian countries, was conducted. Nine studies included fewer than 1,000 subjects, raising questions about the representativeness of the samples and the reliability of the reported results. The data regarding the relationship between ABSI and cardiometabolic risk remain controversial. Even when applying age- and sex-adjusted ABSI, and when compared with other established or newly introduced anthropometric measurements, the discriminatory capacity of ABSI remains uncertain.</p><p><strong>Conclusions: </strong>The findings to date highlight the variable discriminating capacity of ABSI as a predictor of cardiometabolic risk in children and adolescents.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 5","pages":"54-60"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11784880/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143083095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictors of Satisfactory Surgical Outcome in Idiopathic Normal Pressure Hydrocephalus (Review).","authors":"A V Stanishevskiy, G V Gavrilov, M N Radkov, B G Adlejba, D V Svistov","doi":"10.17691/stm2024.16.2.07","DOIUrl":"10.17691/stm2024.16.2.07","url":null,"abstract":"<p><p>Idiopathic normal pressure hydrocephalus is a widespread neurodegenerative disease of the elderly. If not treated surgically early, it results in a severe decrease in quality of life and disability. According to current clinical Russian and foreign guidelines the candidates for CSF shunting procedures are selected based on the results of invasive tests, though treatment outcomes are not always optimal. At the same time, in the last decade there have been published a number of studies on promising noninvasive diagnosis and prognosis of the surgical treatment of idiopathic normal pressure hydrocephalus based on neuroimaging findings. <b>The aim of the present systematic review</b> is to demonstrate the most promising imaging predictors of satisfactory outcomes of CSF shunting procedures in patients with idiopathic normal pressure hydrocephalus based on published literature data.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 2","pages":"68-77"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11556049/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Synthesis of Bombesin-Based Radiopharmaceutical Precursors Modified with Knottin.","authors":"E A Beloborodov, E V Iurova, A N Fomin, Yu V Saenko","doi":"10.17691/stm2024.16.2.01","DOIUrl":"10.17691/stm2024.16.2.01","url":null,"abstract":"<p><p>Bombesin receptors on the cell surface are of great interest as a target for targeted cancer therapy. One of the strategies of targeting bombesin receptors involves the use of tropic short peptides. However, the main limitation for the wide application of peptides as drugs is their low stability <i>in vivo</i> due to their sensitivity to extreme conditions of the internal body environment such as temperature and action of enzymes. In our work, a short bombesin peptide, taken as a basis, was modified with a knottin, a toxin with an inhibitor cystine knot, increasing thereby the stability of the short peptide under various conditions. <b>The aim of the investigation</b> is to study the chemical and radiochemical stability of the structure based on the short bombesin peptide and knottin, as well as the ability of the obtained structure to bind to tumor cells.</p><p><strong>Materials and methods: </strong>The work analyzed the chemical and radiochemical stability of the synthesized peptide labeled with a lutetium radioisotope using high-performance liquid chromatography. A fluorescent-labeled peptide, obtained by a solid-phase peptide synthesis, was used to analyze binding to cultures expressing bombesin receptors.</p><p><strong>Results: </strong>The analysis has shown increased chemical and radiochemical stability of the knottin-modified peptide, as compared to the commercial analog, and maintenance of a high ability to bind to receptors on the surface of cancer cells.</p><p><strong>Conclusion: </strong>The structure created on the basis of a short bombesin peptide and knottin possesses increased stability and retains the ability to bind to cancer cells. All this allows us to consider the creation of these structures as a strategy for fabricating stabilizing scaffolds for short peptides for a peptide-receptor therapy.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 2","pages":"5-14"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11556054/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of DNA Microarray for Parallel Detection of Community-Acquired Pneumonia Bacterial Pathogens.","authors":"N A Sakharnov, E N Filatova, M I Popkova, S L Slavin, O V Utkin","doi":"10.17691/stm2024.16.2.02","DOIUrl":"10.17691/stm2024.16.2.02","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;The aim of the study&lt;/b&gt; was to develop an experimental version of a DNA microarray for parallel detection of community-acquired pneumonia bacterial pathogens.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Materials and methods: &lt;/strong&gt;We studied the samples of the pharyngeal mucosa smears taken from children aged 1-15 years with X-ray confirmed pneumonia. The selection of DNA probes for specific detection of community-acquired pneumonia pathogens (&lt;i&gt;S. pneumoniae&lt;/i&gt;, &lt;i&gt;H. influenzae&lt;/i&gt;, &lt;i&gt;M. pneumoniae&lt;/i&gt;, &lt;i&gt;C. pneumonia&lt;/i&gt;, and &lt;i&gt;L. pneumophila&lt;/i&gt;) and development of the microarray design were carried out using the disprose program. The nucleotide sequences of pathogens were obtained from NCBI Nucleotide database. In the research we used CustomArray microarrays (USA). For a pooled sample containing &lt;i&gt;S. pneumoniae&lt;/i&gt; and &lt;i&gt;H. influenzae&lt;/i&gt; DNA, we performed a sequential selection of the best combinations of hybridization parameters: DNA fragment size, DNA amount, hybridization temperature. The selection criteria were: the percentage of effective probes with a standardized hybridization signal (SHS) ≥3 &lt;i&gt;Z&lt;/i&gt;, and the excess of SHS levels of effective specific probes compared to SHS of effective nonspecific probes. We selected the probes to detect of &lt;i&gt;S. pneumoniae&lt;/i&gt; and &lt;i&gt;H. influenzae&lt;/i&gt; characterized by an effective hybridization signal under optimal conditions. The developed microarray was tested under the selected conditions on clinical samples containing &lt;i&gt;S. pneumoniae&lt;/i&gt; or &lt;i&gt;H. influenzae&lt;/i&gt; DNA. Using ROC analysis there were established threshold values for the signals of specific probes at optimal sensitivity points and the test specificity, the excess of which was interpreted as the evidence of pathogen presence in a sample.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;A microarray design included 142 DNA probes to detect &lt;i&gt;S. pneumoniae&lt;/i&gt;, &lt;i&gt;H. influenzae&lt;/i&gt;, &lt;i&gt;M. pneumoniae&lt;/i&gt;, &lt;i&gt;C. pneumoniae&lt;/i&gt;, and &lt;i&gt;L. pneumophila&lt;/i&gt;, the probes being synthesized onto slides. Using the example of clinical samples containing &lt;i&gt;S. pneumoniae&lt;/i&gt; and/or &lt;i&gt;H. influenza&lt;/i&gt; DNA, we selected optimal parameters for DNA hybridization on microarrays, which enabled to identify bacterial pathogens of community-acquired pneumonia with sufficient efficiency, specificity and reproducibility: the amount of hybridized DNA was 2 μg, the DNA fragment size: 300 nt, hybridization temperature: 47°C. There was selected a list of probes for specific detection of &lt;i&gt;S. pneumoniae&lt;/i&gt; and &lt;i&gt;H. influenzae&lt;/i&gt; characterized by an effective hybridization signal under the identified conditions. We determined the threshold values of standardized probe signals for specific detection of &lt;i&gt;S. pneumoniae&lt;/i&gt; (4.5 &lt;i&gt;Z&lt;/i&gt;) and &lt;i&gt;H. influenzae&lt;/i&gt; (4.9 &lt;i&gt;Z&lt;/i&gt;) in clinical samples.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;A DNA microarray was developed and synthesized for parallel indication of bacterial pathogens of community-acquired pneumonia. There were selected the optim","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 2","pages":"16-26"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11556048/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}