Sovremennye tekhnologii v meditsine最新文献

{"title":"Visualization of Gastric Adenocarcinoma Lymph Node Metastases by Microscopy with Ultraviolet Surface Excitation.","authors":"G M Denisenko, Y M Valieva, S E Solovyeva, N B Serejnikova, V A Petrov, G S Budylin, P S Timashev, A L Fayzullin","doi":"10.17691/stm2024.16.6.03","DOIUrl":"10.17691/stm2024.16.6.03","url":null,"abstract":"<p><p>The detection of lymph node metastases is crucial in oncopathology, as it makes it possible to determine the TNM stage, to design a treatment plan, and predict the survival for cancer patients. The current gold standard for this process is hematoxylin and eosin staining. However, new alternative methods leveraging the unique optical properties of tissue structures are being developed for rapid intraoperative or postoperative application. <b>The aim of the study</b> is to evaluate the effectiveness of identifying lymph node metastases using microscopy with ultraviolet surface excitation (MUSE).</p><p><strong>Materials and methods: </strong>17 lymph nodes from the Sechenov University archive (Russia) collected intraoperatively from 6 patients with gastric cancer have been investigated.In this study, we utilized a MUSE optical system consisting of three UV light-emitting diodes (265 nm) and the Axio Scope A1 microscope (Carl Zeiss, Germany) with various objectives. We introduced a novel combination of fluorescent dyes - Nile red and Hoechst - that had not been previously used with MUSE.</p><p><strong>Results: </strong>The combination of fluorescent dyes yielded high-contrast images with blue-stained nuclei and orange-to-red stained cytoplasm, effectively visualizing gastric adenocarcinoma cells characterized by abundant cytoplasmic components and large polymorphic nuclei. The presence of irregularly shaped cavities, formed by adenocarcinoma metastases, was also detectable by MUSE.</p><p><strong>Conclusion: </strong>Biophotonics provides alternative methods for tissue imaging. However, traditional methods are still unsurpassed in the accuracy of detecting cancer metastases and other pathologies. Further refinement of imaging protocols and expanded research into other cancer types are needed to make methods like MUSE applicable for intraoperative diagnosis.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 6","pages":"25-32"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11780589/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143082933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Deep Learning Algorithms Based on the Multilayer Y0L0v8 Neural Network to Identify Fungal Keratitis.","authors":"A V Sitnova, E R Valitov, S N Svetozarskiy","doi":"10.17691/stm2024.16.4.01","DOIUrl":"https://doi.org/10.17691/stm2024.16.4.01","url":null,"abstract":"<p><p><b>The aim of the study</b> is to develop a method for diagnosing fungal keratitis based on the analysis of photographs of the anterior segment of the eye using deep learning algorithms with subsequent evaluation of sensitivity and specificity of the method on a test data set in comparison with the results of practicing ophthalmologists.</p><p><strong>Materials and methods: </strong>The study has included the stages of data acquisition, image pre-training and markup, selection of training approach and neural network architecture, training with input data augmentation, validation with hyperparameter correction, evaluation of algorithm performance on a test sample, and determination of sensitivity and specificity of fungal keratitis detection by practicing doctors. A total of 274 anterior segment images were used, including 130 photographs of the eyes affected by fungal keratitis and 144 photographs illustrating normal eyes, keratitis of other etiologies, and various anterior segment pathologies. Photographs taken after the treatment onset, illustrations of keratitis of mixed etiology and corneal perforation were excluded from the study. Images of the training sample were marked up using the VGG Image Annotator web application and then used to train the YOLOv8 convolutional neural network. Images from the test data set were also offered to practicing ophthalmologists to determine the diagnostic accuracy of fungal keratitis.</p><p><strong>Results: </strong>The sensitivity of the model was 56.0%, the specificity level reached 96.1%, and the proportion of correct answers of the algorithm was 76.5%. The accuracy of image recognition by practicing ophthalmologists was 50.0%, specificity - 41.7%, sensitivity - 57.7%.</p><p><strong>Conclusion: </strong>The study showed the high potential of deep learning algorithms in the diagnosis of fungal keratitis and its advantages in accuracy compared to expert judgment in the absence of metadata. The use of computer vision technologies may find application as a complementary diagnostic method in decision making in complex cases and in telemedicine care settings. Further research is required to compare the developed model with alternative approaches, to expand and standardize databases.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 4","pages":"5-13"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11773139/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143070657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experience of Using Neural Networks to Assess Age-Related Changes in Some Structures of the Skull and Cervical Vertebrae Based on CT Scans (Pilot Project).","authors":"G V Zolotenkova, D K Valetov, M P Poletaeva, Yu V Vassilevski","doi":"10.17691/stm2024.16.2.03","DOIUrl":"10.17691/stm2024.16.2.03","url":null,"abstract":"<p><p><b>The aim of the investigation</b> is to study the possibility of using artificial intelligence technologies for age prediction based on CT studies of some structures of the skull and cervical vertebrae.</p><p><strong>Material and methods: </strong>The study included 223 tomograms of the head and neck in sagittal projection from patients without any pathology of the studied structures. Morphometric analysis was carried out using PjaPro and Gradient programs, statistical analysis was performed by SPSS Statistics software. A fully convolutional EfficientNet-B2 neural network was used, which was trained in two stages: selection of the area of interest and solution of regression tasks.</p><p><strong>Results: </strong>Morphometric assessment and subsequent statistical analysis of the selected group of features have shown presence of the strongest correlation with age in the indicator characterizing the involution of the median atlantoaxial joint. A deep learning method using the convolutional network, which automatically selects the desired area in the image (the area of the vertebral junction), classifies the sample, and makes an assumption about the age of the unknown individual with an accuracy of 7.5 to 10.5 years has been tested.</p><p><strong>Conclusion: </strong>As a result of the study, a positive experience has been obtained indicating the possibility of using convolutional neural networks to determine the age of the unknown person, which expands the evidence base and provides new opportunities for determining group-wide personality traits in forensic medicine.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 2","pages":"29-38"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11556052/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-adhesive Properties of Epoxy-Treated Xenopericardium Modified with Polyvinyl Alcohol: <i>in vitro</i> Study of Leukocyte Adhesion in the Pulsatile Flow Model.","authors":"E A Ovcharenko, T V Glushkova, D K Shishkova, M A Rezvova, E A Velikanova, K Yu Klyshnikov, T N Akentyeva, A E Kostyunin","doi":"10.17691/stm2024.16.2.04","DOIUrl":"10.17691/stm2024.16.2.04","url":null,"abstract":"<p><p><b>The aim of the study</b> is to assess protective capabilities of the polymer coating made of polyvinyl alcohol to prevent leukocyte adhesion to epoxy-treated bovine pericardium, which is used in production of bioprosthetic heart valves.</p><p><strong>Materials and methods: </strong>Fragments of unmodified (control) and modified with polyvinyl alcohol epoxy-treated bovine pericardium were incubated in the dedicated chambers connected to a pulsatile flow system (Ibidi GmbH, Germany). During 48 h incubation was conducted in whole donor plasma containing 3<b>·</b>10⁶ of mononuclear fraction cells. To simulate plasma flow, the shear stress on the inflow and outflow sides of of bioprosthetic heart valve in the aortic position was set to 50 and 20 dynes/cm², respectively. After the experiment was completed, the surface of the studied samples was subjected to scanning electron microscopy and immunofluorescence using antibodies to the pan-leukocyte marker CD45.</p><p><strong>Results: </strong>Adhesion of leukocytes (CD45⁺) was seen for both the serous (outflow side) and fibrous (inflow side) surfaces of the control epoxy-treated bovine pericardium, whereas both surfaces of the material modified with polyvinyl alcohol were clear of immune cells. Scanning electron microscopy confirmed the adhesion of leukocytes to intact biological tissue: the cells on the surface of the control xenopericardium were of an irregular shape and formed numerous pseudopodia.</p><p><strong>Conclusion: </strong>The suggested modification of epoxy-treated bovine pericardium with polyvinyl alcohol prevents the adhesion of immune cells to the implant surface and can potentially protect bioprosthetic heart valves from immune rejection.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 2","pages":"40-44"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11556051/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142636182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Skin Recovery after Fractional CO₂ Laser Treatment Lichen Sclerosus Using Multimodal Optical Coherence Tomography.","authors":"M A Sirotkina, A L Potapov, M M Loginova, A E Bychkova, A A Moiseev, M V Kochuyeva, A Yu Bogomolova, E B Kiseleva, A V Asaturova, A V Maslennikova, S G Radenska-Lopovok, L A Apolikhina, N D Gladkova","doi":"10.17691/stm2024.16.4.02","DOIUrl":"10.17691/stm2024.16.4.02","url":null,"abstract":"<p><p><b>The aim of the study</b> is to demonstrate the effect of fractional CO₂ laser treatment on vulvar skin in lichen sclerosus using multimodal optical coherence tomography (MM OCT).</p><p><strong>Materials and methods: </strong>The study included 3 clinical cases of vulvar lichen sclerosus (VLS) (histologically classified as early, early with dermal edema, late) and 2 control cases without vulvar pathology. Patients with VLS underwent 3 procedures of fractional CO₂ laser treatment with an interval of 30-40 days. In patients without vulvar pathology, the MM OCT examination was performed once, in patients with VLS it was done at the point of the greatest visible skin damage just before a punch biopsy, immediately after the first laser session, and 1 month after a full course of treatment.</p><p><strong>Results: </strong>Analyzing the case series of vulvar skin recovery 1 month after fractional CO₂ laser treatment, it has been found that the recovery depended on the dermal condition before the treatment. In early VLS and early VLS with dermal edema (clinical cases 1 and 2) before the treatment, the MM OCT examination has shown a decreased epidermal thickness, unclear contrast between the epidermis and dermis, a decrease in the values of the OCT signal attenuation coefficient in the dermis, and a sharp reduction in the density of blood and lymphatic vessels. After treatment, the MM OCT examination demonstrated a complete recovery of vulvar skin structure and all quantitative values reached the level characteristic of normal vulvar skin. Before the treatment, a homogenization zone (sclerosis) was clearly visible in the MM OCT images of the dermis in late VLS (clinical case 3). After the treatment, complete recovery of the vulvar skin structure was not observed; the area of sclerosis was preserved; however, visually, there was an increase in the density of blood and lymphatic vessels in the affected area, which almost reached the level of normal vulvar skin.</p><p><strong>Conclusion: </strong>MM OCT can be proposed as a promising non-invasive method for monitoring skin recovery after fractional CO₂ laser treatment of VLS.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 4","pages":"15-26"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11773141/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143070670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential of Current Direct Mechanical Testing Methods in Assessing Intraoperative Samples of Aortic Aneurysm Caused by Uncontrolled Arterial Hypertension.","authors":"V N Nikolenko, Y V Belov, M V Oganesyan, Y M Efremov, N A Rizaeva, A D Vovkogon, A V Sankov, L A Gridin, P S Timashev, K V Bulygin, M V Sankova","doi":"10.17691/stm2024.16.4.05","DOIUrl":"10.17691/stm2024.16.4.05","url":null,"abstract":"<p><p><b>The aim of the study</b> was to investigate the potential of direct mechanical testing methods in clinical practice to assess the strength and elastic-deformative characteristics of intraoperative samples of aortic arch aneurysm caused by uncontrolled arterial hypertension.</p><p><strong>Materials and methods: </strong>The study experimental material was the resected parts of the aortic aneurysm obtained during aneurysm replacement surgery in a patient with uncontrolled arterial hypertension. The direct mechanical testing methods such as instrumental indentation and uniaxial extension were used.</p><p><strong>Results: </strong>It was shown that by the direct instrumental indentation it is possible to accurately assess and compare the stiffness of all three layers of the aortic wall. In this clinical case, the inner aorta layer was subject to the greatest atherosclerotic damage. In the media area, the values of this indicator were widely scattered, whereas the material was greatly dissected. By uniaxial extension method it is possible to obtain accurate parameters of the vascular wall strength, as well as to assess the stiffness, elasticity, and deformability of the intraoperatively resected aortic tissue. It was found that the aneurysm aortic wall, compared with the non-dilated aortic section, was characterized by a significantly lower strength in both longitudinal (by 4.25 times) and transverse (by 3.75 times) directions. In addition, aneurysm tissues demonstrated a significantly lower elasticity and deformability.</p><p><strong>Conclusion: </strong>The study demonstrated the perspectives and options of using in clinical practice current methods of direct mechanical testing, which makes it possible to obtain more accurate indicators of the strength and elastic-deformative vascular characteristics, to clarify the pathophysiological mechanisms of cardiovascular accidents, and to justify the need for regular monitoring of vascular wall stiffness in clinical practice, in particular in patients with uncontrolled arterial hypertension.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 4","pages":"46-53"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11773140/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143070671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Capabilities of Cephalometric Methods to Study X-rays in Three-Dimensional Space (Review).","authors":"I O Ayupova, A Yu Makhota, A V Kolsanov, N V Popov, M A Davidyuk, I A Nekrasov, P A Romanova, A M Khamadeeva","doi":"10.17691/stm2024.16.3.07","DOIUrl":"10.17691/stm2024.16.3.07","url":null,"abstract":"<p><p><b>The aim of the study</b> was a systematic review of modern methods of three-dimensional cephalometric analysis, and the assessment of their efficiency. The scientific papers describing modern diagnostic methods of MFA in dental practice were searched in databases PubMed, Web of Science, eLIBRARY.RU, as well as in a searching system Google Scholar by the following key words: three-dimensional cephalometry, three-dimensional cephalometric analysis, orthodontics, asymmetric deformities, maxillofacial anomalies, 3D cephalometry, CBCT. The literature analysis showed many methods of cephalometric analysis described as three-dimensional to use two-dimensional reformates for measurements. True three-dimensional methods are not applicable for practical purposes due to the fragmentary nature of the studies. There is the disunity in choosing landmarks and supporting planes that makes the diagnosis difficult and costly. The major issue is the lack of uniform standards for tree-dimensional measurements of anatomical structures of the skull, and the data revealed can be compared to them. In this regard, the use of artificial neuron networks and in-depth study technologies to process three-dimensional images and determining standard indicators appear to be promising.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 3","pages":"62-73"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11618529/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142804411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Digital Electrocardiographic Complex for Risk Stratification of Paroxysmal Atrial Fibrillation.","authors":"A V Frolov, O P Melnikova, A P Vorobiev, T G Vaikhanskaya","doi":"10.17691/stm2024.16.3.05","DOIUrl":"10.17691/stm2024.16.3.05","url":null,"abstract":"<p><p><b>The aim of the study</b> was to develop and clinically test a hardware and software system capable of identifying the predictors of the hidden forms of atrial fibrillation (AF) using 12-lead ECG data in sinus rhythm.</p><p><strong>Materials and methods: </strong>There was developed the hardware and software system \"Intecard 8.1\" to assess a set of markers for atrial electrical instability by 3-5-minute ECG recordings in sinus rhythm. The markers include P-wave amplitude in lead II <0.1 mV, P-wave duration >120 ms, advanced interatrial block, the area of the biphasic P-wave terminal part <-4 mV·ms, and MVP (morphology-voltage- P-wave duration) score >3 points.The clinical testing of \"Intecard 8.1\" system was carried out on 120 patients with ischemic heart disease or dilated cardiomyopathy. The patients' average age was 57.9±13.1 years.</p><p><strong>Results: </strong>P-wave detection is a challenging task due to a low signal amplitude, noise, high error probability in atrioventricular block or T-wave and P-wave superposition in case of marked tachycardia. To improve detection, a phase transformation method was used, according to which there was studied its phase component arctg[<i>x</i>(<i>n</i>)<i>/Rv</i>], where <i>x</i>(<i>n</i>) <i>-</i> ECG signal samples, <i>Rv -</i> a constant. We developed an identification algorithm implemented in \"Intecard 8.1\" software, its clinical trials being conducted.During the 12 [6; 22] month observation period, AF episodes were recorded in 22 from 120 patients (18.3%). The patients with AF episodes exhibited a significant decrease in P-wave amplitude (p=0.029), its duration increase (p<0.001), and a significantly high MVP score (p<0.01). The MVP score with a cut-off point >3 points is of the highest prognostic significance. The area under the ROC curve AUC was 0.988 with a 95% confidence interval: 0.975-0.999 (p<0.001). The prediction model of hidden AF paroxysms has sensitivity and specificity: 92 and 89%, respectively.</p><p><strong>Conclusion: </strong>The digital electrocardiographic complex \"Intecard 8.1\" when analyzing 3-5-minute ECG recordings with sinus rhythm enables to identify the patients with high risk or with hidden AF forms. The dynamic assessment of P-wave parameters offers an opportunity to personalize heart rhythm control in this patient cohort.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 3","pages":"43-48"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11618526/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142804412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radiomics and Quantitative MDA Criteria in Breast Cancer with Bone Metastases by MRI: Examples of Calculation Algorithms and Their Practical Use.","authors":"V Steinhauer, G Hartung","doi":"10.17691/stm2024.16.3.01","DOIUrl":"10.17691/stm2024.16.3.01","url":null,"abstract":"<p><p>In the practical work of a radiologist or oncologist, especially in the context of individualized treatment, there is a need for fast and in-depth diagnostics. Radiomics and artificial intelligence can be of great help here. Quantitative and qualitative assessments obtained in this way act as decision support for the physician. <b>The aim of the study</b> is to enhance the ability of quantitative and qualitative assessment of metastatic spinal lesions in breast cancer, to better evaluate the nature of changes under the influence of therapy, in addition to MDA.</p><p><strong>Materials and methods: </strong>We used MRI data in sagittal projection for a patient diagnosed with T2N3M1 breast cancer when treated according to accepted clinical protocols. Metastases to the spine were assessed by a radiologist and by machine analysis using the described software: image internal structure extraction operators and recognition based on traditional neural networks. Fragments of the program codes used are also given.</p><p><strong>Results: </strong>The structure of metastatically changed vertebrae in sagittal projection was analysed using machine operators of image analysis. Subtle changes in structure such as several types of \"calderas\" and the pattern of change in image complexity as treatment with CDK 4/6 inhibitors were detected. Measurements were supported by metastasis recognition using neural networks, to increase the reliability of the estimates. In addition to the ability to record response to therapy, a fundamental ability to assess the degree of action compared to previous therapy was identified.</p><p><strong>Conclusion: </strong>The study showed high efficiency of using image structure analysis algorithms, good correlation of the results obtained with the radiologist's opinion and with clinical and laboratory data, and allowed to approach the analysis of subtle effects to obtain not only quantitative characteristics in addition to MDA, but also to obtain new qualitative results.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 3","pages":"5-10"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11618525/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142804419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Model of Crohn's Disease on Large Laboratory Animals - Pigs.","authors":"E N Fedulova, S N Saralov, O V Shumilova, N Yu Shirokova, K N Ilyina, E A Farafontova, Yu P Rubtsova, M N Egorikhina","doi":"10.17691/stm2024.16.6.04","DOIUrl":"10.17691/stm2024.16.6.04","url":null,"abstract":"<p><p>Dissatisfactory results of treating inflammatory bowel disease require the development of new modern methods of therapy. <b>The aim of the study</b> is to create a model of Crohn's disease on large animals, in which ulcerative defects can be formed, for testing new treatment techniques and assessing their effectiveness by endoscopic and morphological methods.</p><p><strong>Materials and methods: </strong>The model was created and tested on 12 castrated male pigs (hybrids between the Wiesenau and the Vietnamese black potbellied pigs), aged 6 months. The animals were manipulated under general sedation in the operating room of the SPF-vivarium for large laboratory animals at Privolzhsky Research Medical University (Russia). Endoscopic techniques and a highfrequency electrosurgical apparatus were used to create the required defects. The results were assessed endoscopically and with histological and morphometrical techniques on days 7, 14, and 21.</p><p><strong>Results: </strong>The morphological examination of the pigs' intestinal mucous membrane has detected the signs typical of Crohn's disease, demonstrating the possibility of using pigs as a model of ulcerative defects in Crohn's disease.</p><p><strong>Conclusion: </strong>This model of Crohn's disease on large animals (pigs, in particular) significantly widens the borders of using new treatment techniques at the preclinical stage and will improve therapy effectiveness in patients with this disease reducing the risk of surgical intervention.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 6","pages":"34-42"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11780586/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143082776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}