Sovremennye tekhnologii v meditsine最新文献

{"title":"Total Pancreatectomy with Islet Autotransplantation (TPIAT) as a Technique to Treat Chronic Idiopathic Pancreatitis: Early Experience Analysis.","authors":"V E Zagainov, D M Kuchin, A V Kashina, L A Lugovaya, N V Zarechnova, T A Galanina, N U Naraliev, Y I Kolesnik, E A Vasilchikova, P S Ermakova, E M Zagaynov, Yu A Kucheryavy, A Yu Bogomolova, A L Potapov, I Yu Shirokova","doi":"10.17691/stm2024.16.6.05","DOIUrl":"10.17691/stm2024.16.6.05","url":null,"abstract":"<p><p>Chronic pancreatitis (CP) is a quite common disease with an annual incidence of up to 30 new cases per 100,000 persons in Russia, and 9.6 cases per 100,000 persons abroad. Idiopathic pancreatitis associated with genetic mutations ranks second in frequency. When conservative therapy is ineffective, surgical intervention is considered. Total pancreatectomy reduces pain, but leads to pancreatogenic diabetes mellitus. In case of hereditary pancreatitis caused by mutations in the <i>PRSS1</i>, <i>CFTR</i>, <i>SPINK1</i> genes, pancreatectomy becomes advisable, including for prevention of pancreatic cancer. Isolation of Langerhans islets from the excised pancreas and their autotransplantation (total pancreatectomy with islet autotransplantation, TPIAT) provides additional treatment options. Such surgical interventions are not performed in Russia as of yet. <b>The aim of the study</b> was to assess the effectiveness of total pancreatectomy followed by restoration of glucose tolerance in treatment of patients with chronic genetically determined pain pancreatitis.</p><p><strong>Materials and methods: </strong>Two patients with chronic pain pancreatitis with the <i>SPINK1</i> and <i>PRSS1</i> genetic mutations were examined and underwent surgical total pancreatectomy. Islets were isolated from the excised glands and implanted into the liver. Postoperative followup included an assessment of quality of life and pain intensity based on questionnaires, as well as determination of the glycemic level.</p><p><strong>Results: </strong>Following total pancreatoduodenectomy and autotransplantation, a significant decrease in pain and an improvement in quality of life were noted. Transplanted islets' function was reduced, due to their insufficient number, which required administration of exogenous insulin.</p><p><strong>Conclusion: </strong>The described experience demonstrates the TPIAT effectiveness in treatment of chronic pancreatitis, which can become a basis for further research and introduction of the technique into domestic clinical practice.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 6","pages":"44-56"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11780585/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143082874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stereotactic Photodynamic Therapy of Recurrent Malignant Gliomas.","authors":"A A Rafaelian, B V Martynov, K A Chemodakova, A I Kholyavin, R S Martynov, E Yu Klimenkova, M Yu Prokudin, G V Papayan, I V Boykov, D V Svistov","doi":"10.17691/stm2024.16.2.06","DOIUrl":"10.17691/stm2024.16.2.06","url":null,"abstract":"<p><p><b>The aim of the study</b> is to assess the effectiveness and safety of stereotactic photodynamic therapy (sPDT) with 5-aminolevulinic acid (5-ALA) in patients with recurrent malignant supratentorial gliomas in functionally relevant brain areas.</p><p><strong>Materials and methods: </strong>In a retrospective single-center study the results of sPDT with 5-ALA in 10 patients (6 of 10 were male), aged 30 to 62 years (median: 51.5 years; 95% CI: 38-59 years) with recurrent malignant brain gliomas after standard therapy who underwent surgery during the period of 2020-2023 were analyzed. sPDT was conducted during 15 min using 5-ALA at a dosage of 20 mg/kg, a diode laser with a wavelength of 635 nm and power of 1 W, and the LFT-02-BIOSPEC unit (BIOSPEC, Russia). Three patients got repeated sPDT after 3, 7, and 15 months due to a relapse. The number of target points and the optimal position for intervention paths were determined according to the data of preoperative stereotactic MRI of the brain with contrast intensification using the CRW Precision stereotactic navigation system (Integra, USA) and intraoperative registration of the area with the highest intensity of protoporphyrin IX fluorescence along the path (according to fluorescence biospectroscopy).</p><p><strong>Results: </strong>Glioblastoma (grade IV, WHO) was diagnosed in 7 patients, anaplastic astrocytoma (grade III, WHO) - in 3 persons. Genetic studies were performed for 9 patients, 7 of them had tumors without the <i>IDH1</i> gene mutation. None of the patients had a combined 1p/19q deletion. The median volume of the contrast-enhancing part of the recurrent tumor was 7.95 cm³ (95% CI: 3.3-13.6 cm³). The median time to relapse after sPDT in patients with anaplastic astrocytomas and glioblastomas was 14.5 and 6.5 months, respectively. The median survival time after sPDT in patients with glioblastomas was 15.8 months (95% CI: 0.5-20.1 months), and in patients with anaplastic astrocytomas - 46.3 months (95%, CI not specified). In the early postoperative period, two patients had motor aphasia and hemiparesis, which further regressed.</p><p><strong>Conclusion: </strong>The results of a small group of patients allow to consider sPDT with 5-ALA as a promising technique to treat patients with recurrent high-grade gliomas in functionally relevant brain areas and require further prospective assessment.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 2","pages":"58-65"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11556050/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Composite Tissue-Engineered Small-Diameter Vascular Grafts Based on Polycaprolactone and Polyurethane with Growth Factors and Atrombogenic Drug Coatings: Surface Ultrastructure, Physical and Mechanical Properties.","authors":"E A Senokosova, E S Prokudina, E S Krivkina, T V Glushkova, E A Velikanova, M Yu Khanova, E A Torgunakova, V G Matveeva, L V Antonova","doi":"10.17691/stm2024.16.5.02","DOIUrl":"10.17691/stm2024.16.5.02","url":null,"abstract":"<p><p>There is a growing need for synthetic small-diameter vascular grafts (<6 mm) for bypass surgery since the majority of currently developed products have demonstrated unacceptable high frequency of thrombosis in preclinical studies. The proprietary composite vascular graft based on a nonwoven polymer with anti-thrombogenic and anti-aneurysm effect and functional activity is aimed at stimulating the formation of vascular neotissue at the implantation site. <b>The aim of the investigation</b> is to study the surface morphology, physical and mechanical properties of the polycaprolactone/ polyurethane (PCL/PU) small-diameter tissue-engineered vascular graft with growth factors and an anti-thrombogenic surface coating.</p><p><strong>Materials and methods: </strong>PCL/PU vascular grafts with growth factor mix (GFmix) were manufactured using the electrospinning method. The hydrogel coating containing iloprost (Ilo) and heparin (Hep) was formed by complexation with polyvinylpyrrolidone. The controls were multilayer vascular grafts of similar composition and nonwoven matrices based on 12% PCL and 12% PU. The surface structure was analyzed with the S-3400N scanning electron microscope (Hitachi, Japan). The physical properties of the surface were determined by the wetting angle method. The mechanical properties were evaluated on a Z series universal testing machine (Zwick/ Roell, Germany). Statistical processing of the data was performed using the GraphPad Prism 8 software.</p><p><strong>Results: </strong>Our new manufacturing technique for the composite PU/PCL/GFmix/^Ilo/Hep graft has eliminated the problem of graft delamination. The inner surface of the graft consisted of interwined microfibers (1.34 [1.15; 2.28] μm thick), nanofibers (790.0 [604.0; 853.5] nm thick), and interpenetrating pores of different diameters (5.4 [3.8; 8.4] μm). The process of coating formation did not affect the fibers and did not seal the pores, the surface retained its hydrophilic properties (θ=68.61±11.85°). The tensile strength (3.45 [3.17; 4.03] MPa) and Young's modulus (4.88 [3.95; 5.80] MPa) of PU/PCL/GFmix/^Ilo/Hep grafts were almost similar to the human internal thoracic artery compared to the multilayer analogs. The PU/PCL/GFmix/^Ilo/Hep grafts were superior to the multilayer PCL/PU/GFmix/^Ilo/Hep grafts in terms of reduced excessive elasticity (to 118.0 [111.0; 125.0]%; p=0.043).</p><p><strong>Conclusion: </strong>The composite functionalized vascular PU/PCL/GFmix/^Ilo/Hep grafts have enhanced characteristics and compliance, which, in turn, increases the probability of their high patency in future preclinical studies.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 5","pages":"18-25"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11784878/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143083099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Poly-3-Hydroxybutyrate Enriched with Simvastatin in Bone Regeneration after Tooth Extraction (Experimental Study).","authors":"K M Salekh, A A Muraev, A A Dolgalev, A B Dymnikov, G A Bonartseva, T K Makhina, D V Chesnokova, V V Voinova, A P Bonartsev, M E Mokrenko, S Y Ivanov","doi":"10.17691/stm2024.16.5.03","DOIUrl":"10.17691/stm2024.16.5.03","url":null,"abstract":"<p><p>Physiological resorption of bone tissue after tooth extraction leads to a decrease in the volume of bone tissue available for implantation and makes it difficult to install dental implants. Preservation of the well after tooth extraction is the solution to this problem, with the choice of bone plastic material playing an important role. The development of an \"ideal\" bone plastic material with osteoinductive properties that promotes reparative bone regeneration remains an urgent task. <b>The aim of the study</b> was to evaluate the regeneration of bone tissue of the alveolar ridge during implantation of a new osteoinductive bone plastic material containing simvastatin into the wells of extracted teeth in sheep using microcomputer tomography.</p><p><strong>Materials and methods: </strong>The study was conducted on 24 adult sheep with a total of 48 teeth removed. 12 wells were filled with material based on poly(3-hydroxybutyrate) (PHB) with simvastatin; 12 wells were filled with PHB-based material without simvastatin, 24 wells were used as a control. Micro-CT was used for comparative analysis of bone tissue formation between the test groups after 3 and 6 months.</p><p><strong>Results: </strong>The results of the study confirm the positive effect of simvastatin released from the PHB-based osteoplastic material on the volume of the formed bone tissue and the total bone volume in the defect area (BV/TV) and bone mineral density (BMD) 3 and 6 months after surgery.</p><p><strong>Conclusion: </strong>The study demonstrated that simvastatin, released from the PHB-based osteoplastic material, has an osteoinductive effect, promoting bone tissue regeneration in the wells left after tooth removal. Higher BV/TV and BMD values in the wells indicate better efficacy of the material in terms of regeneration support.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 5","pages":"27-33"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11784884/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143083070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive Assessment of Combatants' Psychological and Psychophysiological State in Exposure Therapy of Post-Traumatic Stress Disorder Using Virtual Reality.","authors":"L N Kasimova, A N Kuznetsov, I I Kropinova, D V Kuznetsov, M G Volovik, M V Svyatogor, E M Sychugov, G Y Borovskoy, M E Khalak","doi":"10.17691/stm2024.16.5.04","DOIUrl":"10.17691/stm2024.16.5.04","url":null,"abstract":"<p><p><b>The aim of the study</b> was to develop the rehabilitation technology that enables the monitoring of psychophysiological stress markers during exposure therapy of post-traumatic stress disorder in combatants using virtual reality (VR).</p><p><strong>Materials and methods: </strong>The study involved 69 men: 31 combatants (mean age - 35.61±9.13 years) and 38 healthy research subjects - a control group (mean age - 24.68±5.71 years) not engaged in active combat.Post-traumatic stress disorder was diagnosed using Structured Clinical Interview for DSM and Mississippi Scale.We suggested an original hardware and software system for exposure therapy in VR. Stimulus material included a number of virtual scenes: three combat scenes and a non-combat one.Heart rate variability was recorded to control a patient's state during the session. As markers of stressogenic situations we used CS-index (suggested by S.V. Bozhokin), as well as the indices of functional reserve and tension degree of the regulatory systems (according to R.M. Baevskiy).</p><p><strong>Results: </strong>There were revealed three main responses on VR scenes with an original content. The three variants were called as follows: \"anxious\", \"neutral\", and \"inverse\". The suggested methodology enables to continuously monitor psychophysiological parameters during a certain session, and analyze their dynamics within a therapy course.Using calculated indicators by Baevsky makes it possible to classify combatants by adaptive potential at the beginning and at the end of the exposure therapy course in VR; make use of online-control of a patient's functional state in virtual environment, and create conditions for controlled information influence (CS-index by Bozhokin).The preliminary results presented in the study are promising regarding the possibility to choose a personalized program of rehabilitation measures for each response type using a developed hardware and software system. Biological feedback on heart rate variability included in hardware and software system will contribute to train and harness a patient's habit of an operative independent correction of the patient's state.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 5","pages":"35-42"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11784879/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143083101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnosis of Colorectal Cancer and Adenomatous Polyps of the Colon Based on the Level of MicroRNA Expression in the Mucous Membrane (Pilot Clinical Study).","authors":"M V Bagryantsev, A A Yanyshev, M G Ryabkov, A I Abelevich, I L Dezortsev, A V Bazayev","doi":"10.17691/stm2024.16.5.05","DOIUrl":"10.17691/stm2024.16.5.05","url":null,"abstract":"<p><p>The gold standard for colorectal cancer (CRC) diagnosis, colonoscopy with biopsy, is an invasive technique and has some limitations, while the known non-invasive methods do not possess sufficient sensitivity and specificity. The application of microRNA as a diagnostic and prognostic CRC biomarker may compensate for the colonoscopy limitations. However, there are no data in the literature on the existence of real test systems based on the evaluation of microRNA expression. Our pilot study is the first step to creating a test system for CRC diagnosis based on the analysis of microRNA expression in the tissue of the intact colon. <b>The aim of the study</b> is to assess the prospects of using the level of microRNA expression as a supplemental method for diagnosing colorectal cancer and adenomatous polyps.</p><p><strong>Materials and methods: </strong>Patients participating in the study were divided into three groups: group 1 included patients with CRC (n=5), group 2 - patients with polyps in the colon (n=4), and patients without oncological pathology treated for hemorrhoidal disease without exacerbation (n=5) composed group 3.Tissue samples of the intact gut were taken from all patients. In groups 1 and 2, biopsy was performed in the process of right-sided laparoscopic resection of the colon with tumor. Samples of the mucous membrane from the distal part of the rectum in patients of group 3 were also collected intraoperatively; they were operated on using Milligan-Morgan technique. In groups 1 and 2, CRC and polyp samples, respectively, were taken for the analysis additionally to the intact gut area.The test panel included the following microRNAs: hsa-miR-10b-5p, hsa-miR-20a-5p, hsa-miR-141-3p, hsa-miR-181b-5p. The levels of the reference genes were analyzed with the help of real-time polymerase chain reaction using intercalating SYBR Green stain.</p><p><strong>Results: </strong>Expression of hsa-miR-141-3p in the mucous membrane of the colon in patients of groups 1 and 2 (with CRC and polyps, respectively) was statistically significantly higher than in patients without bowel tumors. At the same time, the expression level of hsa-miR-10b-5p was statistically significantly lower in the tumor tissue (cancer or polyps) in comparison with patients of group 3.Lower values of expression in all tested microRNAs have been detected in the CRC tissue relative to the intact mucosa of the same patients. A similar tendency was also observed in patients with adenomatous polyps.</p><p><strong>Conclusion: </strong>The results of the study have shown that of four microRNAs, included into the test panel, hsa-miR-141-3p and hsa-miR-10b-5p were found to have the diagnostic value for identifying tumor colorectal lesions. Thus, our data will assume that supplementing the endoscopic tests of the large intestine by the epigenetic analysis of the mucous membrane is a promising approach to cancer screening procedures.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 5","pages":"45-51"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11784883/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143083103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Risk Factors for Nontuberculous Mycobacterial Pulmonary Disease (Systematic Review).","authors":"P S Sviridov, M M Litvinova, M A Karnaushkina, N N Makaryants, M V Gorbunova","doi":"10.17691/stm2024.16.5.07","DOIUrl":"10.17691/stm2024.16.5.07","url":null,"abstract":"<p><p>This paper is a systematic review of the published data describing genetic risk factors for pulmonary diseases caused by nontuberculous mycobacteria (nontuberculous mycobacterial pulmonary disease - NTM-PD). <b>The aim of the study</b> is to compile a specific list of genetic markers associated with the risk of developing NTM-PD. This literature review was prepared according to PRISMA criteria and was registered in the International Prospective Register of Systematic Reviews (PROSPERO) (registration number CRD42019128569). In the process of work, a great number of articles from PubMed, Google Scholar, and ScienceDirect databases have been studied. Using careful analysis and selection procedures, a list of 14 genetic variants associated with an increased risk of developing NTM-PD was generated. <i>SLC11A1, NLRP3, TLR2, CFTR, IFNGR1, PDCD1</i> genes have been found to refer to these variants as well as variants in the intergenic regions affecting expression of <i>STK17A, IFNL3, TNF, IL10</i> genes. The products of these genes take different roles in regulating the response to various pathogenic factors, and some of them are poorly understood. For a more precise and detailed explanation of the influence of these genetic variants, further studies in patient groups of different populations with the evaluation of different combinations of variants and intergenic interaction are required.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 5","pages":"62-71"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11784881/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143083085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effects of Mucolytic Agents N-Acetylcysteine and Erdosteine on Hemostasis in Humans.","authors":"S Oktar, S Doğru, S Motor, M Demirköse, E Ş Erden","doi":"10.17691/stm2024.16.4.06","DOIUrl":"https://doi.org/10.17691/stm2024.16.4.06","url":null,"abstract":"<p><p>There are some indications in a few studies on animals and humans that the mucolytic agents N-acetylcysteine and erdosteine have a disruptive effect on the coagulation cascade. This study <b>aimed</b> to examine the effects of both erdosteine and N-acetylcysteine on the coagulation pathway.</p><p><strong>Materials and methods: </strong>The research was conducted on patients treated with N-acetylcysteine (600 mg/day) or erdosteine (600 mg/day) for 7-14 days. To evaluate the coagulation and hemogram parameters, blood samples were taken from patients before treatment and on days 7-14 after starting the treatment. Hemogram and coagulation parameters were measured in the venous blood samples.</p><p><strong>Results: </strong>The levels of factor II significantly decreased, and the platelet count significantly increased in patients treated with erdosteine. D-dimer levels reduced and factor VII levels increased in patients treated with N-acetylcysteine, but these changes were not significant (p=0.069 and p=0.062, respectively). Other coagulation and hemogram values did not change in both groups. Erdosteine may have a dualistic effect on the coagulation cascade through its different metabolites.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 4","pages":"55-60"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11773143/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143070673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Technology of Combined Identification of Macrophages and Collagen Fibers in Liver Samples.","authors":"I A Nikitina, V A Razenkova, E A Fedorova, O V Kirik, D E Korzhevskii","doi":"10.17691/stm2024.16.3.03","DOIUrl":"10.17691/stm2024.16.3.03","url":null,"abstract":"<p><p>The importance of identifying pathological changes in the liver both in fundamental researches and in diagnostic practice dictates the necessity to have a convenient method of assessing functional condition of resident macrophages and connective tissue fibers. <b>The aim of the study</b> is to assess the technology of combined histo-immunohistochemical detection of collagen fibers of connective tissue and resident liver macrophages using aniline blue histological stain and available antibodies to the microglial marker, the Iba-1 protein.</p><p><strong>Materials and methods: </strong>Liver samples from adult rats (n=6) have been used in the study. The connective tissue was stained with a 2% aqueous solution of aniline blue (Unisource Chemicals Ltd., India). Monoclonal rabbit antibodies to Iba-1 (Clone JM36-62; ET1705-78; HuaBio, China) were used to detect resident liver macrophages, zinc-ethanol-formaldehyde was employed as a fixative.</p><p><strong>Results: </strong>The combined staining method allowed us to detect numerous Iba-1-immunopositive structures corresponding morphologically to Kupffer cells and connective tissue macrophages, background staining was not observed. Staining with aniline blue in the liver samples was selective, uniform, and clear, and allowed for differentiation of the connective tissue in all examined samples. Exclusion of the heat-induced epitope retrieval stage caused no negative effect on identification of macrophages, reduced the probability of non-specific staining of the collagen fibers with aniline blue, and ensured general preservation of tinctorial properties of the liver tissue.</p><p><strong>Conclusion: </strong>The presented protocol of combined histo-immunohistochemical identification of Kupffer cells and connective tissue fibers, applied on the rat liver samples, makes it possible to perform effectively the morphometric analysis and may find its application in pathohistological, clinical, and preclinical investigations.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 3","pages":"24-29"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11618527/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142804420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of a Body Shape Index as an Anthropometric Predictor of Cardiometabolic Risks in Children and Adolescents (Systematic Review).","authors":"S A Todorova","doi":"10.17691/stm2024.16.5.06","DOIUrl":"10.17691/stm2024.16.5.06","url":null,"abstract":"<p><p>A body shape index (ABSI) is a novel anthropometric measure associated with body obesity. However, the research on the relationship between the new anthropometric risk index and metabolic disorders and cardiovascular alterations in the pediatric population is limited. <b>The aim of the study</b> was to perform a systematic review and meta-analysis of the associations between cardiometabolic risk and ABSI and evaluate its discrimination capacity.</p><p><strong>Materials and methods: </strong>A systematic search and analysis of the available scientific literature were conducted to identify relevant articles published up to March 2024 in Web of Science, Science Direct, Scopus, ResearchGate, and PubMed. All full-text publications reporting an association between cardiometabolic risk and ABSI in children and adolescents were considered eligible and reviewed in detail. The reported results and statements were summarized using meta-analysis methods.</p><p><strong>Results: </strong>A systematic review of 17 cross-sectional studies involving 31,849 children and adolescents, predominantly from Asian countries, was conducted. Nine studies included fewer than 1,000 subjects, raising questions about the representativeness of the samples and the reliability of the reported results. The data regarding the relationship between ABSI and cardiometabolic risk remain controversial. Even when applying age- and sex-adjusted ABSI, and when compared with other established or newly introduced anthropometric measurements, the discriminatory capacity of ABSI remains uncertain.</p><p><strong>Conclusions: </strong>The findings to date highlight the variable discriminating capacity of ABSI as a predictor of cardiometabolic risk in children and adolescents.</p>","PeriodicalId":520289,"journal":{"name":"Sovremennye tekhnologii v meditsine","volume":"16 5","pages":"54-60"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11784880/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143083095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}