Plant Reproduction最新文献

{"title":"Non-photoperiodic transition of female cannabis seedlings from juvenile to adult reproductive stage.","authors":"Ben Spitzer-Rimon,&nbsp;Hadas Shafran-Tomer,&nbsp;Gilad H Gottlieb,&nbsp;Adi Doron-Faigenboim,&nbsp;Hanita Zemach,&nbsp;Rina Kamenetsky-Goldstein,&nbsp;Moshe Flaishman","doi":"10.1007/s00497-022-00449-0","DOIUrl":"https://doi.org/10.1007/s00497-022-00449-0","url":null,"abstract":"<p><strong>Key message: </strong>Vegetative-to-reproductive phase transition in female cannabis seedlings occurs autonomously with the de novo development of single flowers. To ensure successful sexual reproduction, many plant species originating from seedlings undergo juvenile-to-adult transition. This phase transition precedes and enables the vegetative-to-reproductive shift in plants, upon perception of internal and/or external signals such as temperature, photoperiod, metabolite levels, and phytohormones. This study demonstrates that the juvenile seedlings of cannabis gradually shift to the adult vegetative stage, as confirmed by the formation of lobed leaves, and upregulation of the phase-transition genes. In the tested cultivar, the switch to the reproductive stage occurs with the development of a pair of single flowers in the 7th node. Histological analysis indicated that transition to the reproductive stage is accomplished by the de novo establishment of new flower meristems which are not present in a vegetative stage, or as dormant meristems at nodes 4 and 6. Moreover, there were dramatic changes in the transcriptomic profile of flowering-related genes among nodes 4, 6, and 7. Downregulation of flowering repressors and an intense increase in the transcription of phase transition-related genes occur in parallel with an increase in the transcription of flowering integrators and meristem identity genes. These results support and provide molecular evidence for previous findings that cannabis possesses an autonomous flowering mechanism and the transition to reproductive phase is controlled in this plant mainly by internal signals.</p>","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"35 4","pages":"265-277"},"PeriodicalIF":3.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10364392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and analysis of the promoter region of monodehydroascorbate reductase 4 (CpMDAR4) in papaya.","authors":"Dessireé Zerpa-Catanho,&nbsp;Steven J Clough,&nbsp;Ray Ming","doi":"10.1007/s00497-022-00447-2","DOIUrl":"https://doi.org/10.1007/s00497-022-00447-2","url":null,"abstract":"<p><strong>Key message: </strong>Differential spatial and temporal expression patterns due to regulatory cis-elements and two different isoforms are detected among CpMDAR4 alleles in papaya. The aim of this research was to study the effects of cis-element differences between the X, Y and Y^h alleles on the expression of CpMDAR4, a potential candidate gene for sex differentiation in papaya, using a transcriptional reporter system in a model species Arabidopsis thaliana. Possible effects of a retrotransposon insertion in the Y and Y^h alleles on the transcription and expression of CpMDAR4 alleles in papaya flowers were also examined. When comparing promoters and cis-regulatory elements among genes in the non-recombining region of the sex chromosomes, paired genes exhibited differences. Our results showed that differences in the promoter sequences of the CpMDAR4 alleles drove the expression of a reporter gene to different flower tissues in Arabidopsis. β-glucuronidase staining analysis of T₂ and T₃ lines for constructs containing 5' deletions of native Y and Y^h allele promoters showed the loss of specific expression of the reporter gene in the anthers, confirming the existence and location of cis-regulatory element POLLEN1LELAT52. The expression analysis of CpMDAR4 alleles in papaya flowers also showed that all alleles are actively expressed in different flower tissues, with the existence of a shorter truncated isoform, with unknown function, for the Y and Y^h alleles due to an LTR-RT insertion in the Y and Y^h chromosomes. The observed expression patterns in Arabidopsis thaliana flowers and the expression patterns of CpMDAR4 alleles in papaya flowers suggest that MDAR4 might have a role on development of reproductive organs in papaya, and that it constitutes an important candidate for sex differentiation.</p>","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"35 4","pages":"233-264"},"PeriodicalIF":3.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10714287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparing the efficiency of six clearing methods in developing seeds of Arabidopsis thaliana.","authors":"Venkata Pardha Saradhi Attuluri,&nbsp;Juan Francisco Sánchez López,&nbsp;Lukáš Maier,&nbsp;Kamil Paruch,&nbsp;Hélène S Robert","doi":"10.1007/s00497-022-00453-4","DOIUrl":"https://doi.org/10.1007/s00497-022-00453-4","url":null,"abstract":"<p><strong>Key message: </strong>ClearSee alpha and FAST9 were optimized for imaging Arabidopsis seeds up to the torpedo stages. The methods preserve the fluorescence of reporter proteins and seed shape, allowing phenotyping embryos in intact seeds. Tissue clearing methods eliminate the need for sectioning, thereby helping better understand the 3D organization of tissues and organs. In the past fifteen years, clearing methods have been developed to preserve endogenous fluorescent protein tags. Some of these methods (ClearSee, TDE, PEA-Clarity, etc.) were adapted to clear various plant species, with the focus on roots, leaves, shoot apical meristems, and floral parts. However, these methods have not been used in developing seeds beyond the early globular stage. Tissue clearing is problematic in post-globular seeds due to various apoplastic barriers and secondary metabolites. In this study, we compared six methods for their efficiency in clearing Arabidopsis thaliana seeds at post-globular embryonic stages. Three methods (TDE, ClearSee, and ClearSee alpha) have already been reported in plants, whereas the others (fsDISCO, FAST9, and CHAPS clear) are used in this context for the first time. These methods were assessed for seed morphological changes, clearing capacity, removal of tannins, and spectral properties. We tested each method in seeds from globular to mature stages. The pros and cons of each method are listed herein. ClearSee alpha appears to be the method of choice as it preserves seed morphology and prevents tannin oxidation. However, FAST9 with 60% iohexol as a mounting medium is faster, clears better, and appears suitable for embryonic shape imaging. Our results may guide plant researchers to choose a suitable method for imaging fluorescent protein-labeled embryos in intact Arabidopsis seeds.</p>","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"35 4","pages":"279-293"},"PeriodicalIF":3.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9705463/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10473275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seedless fruit in Annona squamosa L. is monogenic and conferred by INO locus deletion in multiple accessions","authors":"B. Rodrigues, C. Gasser, S. Pimenta, M. C. Pereira, S. Nietsche","doi":"10.1101/2022.10.25.513714","DOIUrl":"https://doi.org/10.1101/2022.10.25.513714","url":null,"abstract":"Understanding the genetic basis and inheritance of a trait facilitates the planning of breeding and development programs of new cultivars. In the sugar apple tree (Annona squamosa L.), the mechanism of the desirable seedless trait in the Thai seedless (Ts) and Brazilian seedless (Bs) accessions was associated with a deletion of the INNER NO OUTER (INO) locus. Genetic analysis of F1, F2 and backcross descendants of crosses of Bs to fertile wild-type varieties showed that seedlessness was recessive and monogenic. Whole genome sequencing of a third accession, Hawaiian seedless (Hs), identified a 16 kilobase deletion including INO. The finding of an identical deletion in Ts and Bs indicated a common origin among genotypes, from a single deletion event. Analysis of microsatellite markers could not preclude the possibility that all three accessions are vegetatively propagated clones. The sequence of the deletion site enabled formulation of a codominant assay for the wild-type and mutant genes that validated the INO gene deletion as the cause of seedless trait, and can be used in the selection of new seedless varieties. The study findings and obtained progenies should be useful in breeding and introgression programs of the trait into elite sugar apple lines and into other Annonas by means of interspecific crossings.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"1 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2022-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"62353704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomes across fertilization and seed development in the water lily Nymphaea thermarum (Nymphaeales): evidence for epigenetic patterning during reproduction.","authors":"Rebecca A Povilus,&nbsp;William E Friedman","doi":"10.1007/s00497-022-00438-3","DOIUrl":"https://doi.org/10.1007/s00497-022-00438-3","url":null,"abstract":"<p><strong>Key message: </strong>The first record of gene expression during seed development within the Nymphaeales provides evidence for a variety of biological processes, including dynamic epigenetic patterning during sexual reproduction in the water lily Nymphaea thermarum. Studies of gene expression during seed development have been performed for a growing collection of species from a phylogenetically broad sampling of flowering plants (angiosperms). However, angiosperm lineages whose origins predate the divergence of monocots and eudicots have been largely overlooked. In order to provide a new resource for understanding the early evolution of seed development in flowering plants, we sequenced transcriptomes of whole ovules and seeds from three key stages of reproductive development in the waterlily Nymphaea thermarum, an experimentally tractable member of the Nymphaeales. We first explore patterns of gene expression, beginning with mature ovules and continuing through fertilization into early- and mid-stages of seed development. We find patterns of gene expression that corroborate histological/morphological observations of seed development in this species, such as expression of genes involved in starch synthesis and transcription factors that have been associated with embryo and endosperm development in other species. We also find evidence for processes that were previously not known to be occurring during seed development in this species, such as epigenetic modification. We then examine the expression of genes associated with patterning DNA and histone methylation-processes that are essential for seed development in distantly related and structurally diverse monocots and eudicots. Around 89% of transcripts putatively homologous to DNA and histone methylation modifiers are expressed during seed development in N. thermarum, including homologs of genes known to pattern imprinting-related epigenetic modifications. Our results suggest that dynamic epigenetic patterning is a deeply conserved aspect of angiosperm seed development.</p>","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"35 3","pages":"161-178"},"PeriodicalIF":3.4,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10713833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of the nucleotides immediately upstream of the AUG start codon on the efficiency of translation initiation in sperm cells.","authors":"Jiao-Jiao Shi,&nbsp;Yuan Cao,&nbsp;Qiu-Hua Lang,&nbsp;Yao Dong,&nbsp;Liu-Yuan Huang,&nbsp;Liu-Jie Yang,&nbsp;Jing-Jing Li,&nbsp;Xue-Xin Zhang,&nbsp;Dan-Yang Wang","doi":"10.1007/s00497-022-00442-7","DOIUrl":"https://doi.org/10.1007/s00497-022-00442-7","url":null,"abstract":"<p><p>It is widely known that an optimal nucleotide sequence context immediately upstream of the AUG start codon greatly improves the efficiency of translation initiation of mRNA in mammalian and plant somatic cells, which in turn increases protein levels. However, it is still unclear whether a similar regulatory mechanism is also present in highly differentiated cells. Here, we surveyed this issue in Arabidopsis thaliana sperm cells and found that the sequence context-mediated regulation of translation initiation in sperm cells is generally similar to that in somatic cells. A simple motif of four adenine nucleotides at positions - 1 to - 4 greatly improved the efficiency of translation initiation, and when the motif was present there, translation was even initiated at some non-AUG codons in sperm cells. However, unlike that in mammalian cells, a mainly effective nucleotide site to regulate the efficiency of translation initiation was not present at positions - 1 to - 4 in sperm cells. Meanwhile, different from somatic cells, sperm cells did not use eukaryotic translation initiation factor 1 to regulate the efficiency in a poor context consisting of the lowest frequency nucleotides. All these results contribute to our understanding of the cytoplasmic event of translation initiation in highly differentiated sperm cells.</p>","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"35 3","pages":"221-231"},"PeriodicalIF":3.4,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10362421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The TCP transcription factor HvTB2 heterodimerizes with VRS5 and controls spike architecture in barley.","authors":"Tatiana de Souza Moraes,&nbsp;Sam W van Es,&nbsp;Inmaculada Hernández-Pinzón,&nbsp;Gwendolyn K Kirschner,&nbsp;Froukje van der Wal,&nbsp;Sylvia Rodrigues da Silveira,&nbsp;Jacqueline Busscher-Lange,&nbsp;Gerco C Angenent,&nbsp;Matthew Moscou,&nbsp;Richard G H Immink,&nbsp;G Wilma van Esse","doi":"10.1007/s00497-022-00441-8","DOIUrl":"https://doi.org/10.1007/s00497-022-00441-8","url":null,"abstract":"<p><strong>Key message: </strong>Understanding the molecular network, including protein-protein interactions, of VRS5 provide new routes towards the identification of other key regulators of plant architecture in barley. The TCP transcriptional regulator TEOSINTE BRANCHED 1 (TB1) is a key regulator of plant architecture. In barley, an important cereal crop, HvTB1 (also referred to as VULGARE SIX-ROWED spike (VRS) 5), inhibits the outgrowth of side shoots, or tillers, and grains. Despite its key role in barley development, there is limited knowledge on the molecular network that is utilized by VRS5. In this work, we performed protein-protein interaction studies of VRS5. Our analysis shows that VRS5 potentially interacts with a diverse set of proteins, including other class II TCP's, NF-Y TF, but also chromatin remodelers. Zooming in on the interaction capacity of VRS5 with other TCP TFs shows that VRS5 preferably interacts with other class II TCP TFs in the TB1 clade. Induced mutagenesis through CRISPR-Cas of one of the putative VRS5 interactors, HvTB2 (also referred to as COMPOSITUM 1 and BRANCHED AND INDETERMINATE SPIKELET 1), resulted in plants that have lost their characteristic unbranched spike architecture. More specifically, hvtb2 mutants exhibited branches arising at the main spike, suggesting that HvTB2 acts as inhibitor of branching. Our protein-protein interaction studies of VRS5 resulted in the identification of HvTB2 as putative interactor of VRS5, another key regulator of spike architecture in barley. The study presented here provides a first step to underpin the protein-protein interactome of VRS5 and to identify other, yet unknown, key regulators of barley plant architecture.</p>","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"35 3","pages":"205-220"},"PeriodicalIF":3.4,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9352630/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10425231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of assimilate competition during early seed development on the pod and seed growth traits in soybean.","authors":"Mohammad Foteh Ali, Paige Brown, John Thomas, Montserrat Salmerόn, Tomokazu Kawashima","doi":"10.1007/s00497-022-00439-2","DOIUrl":"10.1007/s00497-022-00439-2","url":null,"abstract":"<p><p>Although the seed remains small in size during the initial stage of seed development (the lag phase), several studies indicate that environment and assimilate supply level manipulations during the lag phase affect the final seed size. However, the manipulations were not only at the lag phase, making it difficult to understand the specific role of the lag phase in final seed size determination. It also remained unclear whether environmental cues are sensed by plants and regulate seed development or if it is simply the assimilate supply level, changed by the environment, that affects the subsequent seed development. We investigated soybean (Glycine max L. Merr.) seed phenotypes grown in a greenhouse using different source-sink manipulations (shading and removal of flowers and pods) during the lag phase. We show that assimilate supply is the key factor controlling flower and pod abortion and that the assimilate supply during the lag phase affects the subsequent potential seed growth rate during the seed filling phase. In response to low assimilate supply, plants adjust flower/pod abortion and lag phase duration to supply the minimum assimilate per pod/seed. Our results provide insight into the mechanisms whereby the lag phase is crucial for seed development and final seed size potential, essential parameters that determine yield.</p>","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"35 3","pages":"179-188"},"PeriodicalIF":2.4,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10713835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plastid ribosome protein L5 is essential for post-globular embryo development in Arabidopsis thaliana.","authors":"Gilles Dupouy,&nbsp;Emma McDermott,&nbsp;Ronan Cashell,&nbsp;Anna Scian,&nbsp;Marcus McHale,&nbsp;Peter Ryder,&nbsp;Joelle de Groot,&nbsp;Noel Lucca,&nbsp;Galina Brychkova,&nbsp;Peter C McKeown,&nbsp;Charles Spillane","doi":"10.1007/s00497-022-00440-9","DOIUrl":"https://doi.org/10.1007/s00497-022-00440-9","url":null,"abstract":"<p><p>Plastid ribosomal proteins (PRPs) can play essential roles in plastid ribosome functioning that affect plant function and development. However, the roles of many PRPs remain unknown, including elucidation of which PRPs are essential or display redundancy. Here, we report that the nuclear-encoded PLASTID RIBOSOMAL PROTEIN L5 (PRPL5) is essential for early embryo development in A. thaliana, as homozygous loss-of-function mutations in the PRPL5 gene impairs chloroplast development and leads to embryo failure to develop past the globular stage. We confirmed the prpl5 embryo-lethal phenotype by generating a mutant CRISPR/Cas9 line and by genetic complementation. As PRPL5 underwent transfer to the nuclear genome early in the evolution of Embryophyta, PRPL5 can be expected to have acquired a chloroplast transit peptide. We identify and validate the presence of an N-terminal chloroplast transit peptide, but unexpectedly also confirm the presence of a conserved and functional Nuclear Localization Signal on the protein C-terminal end. This study highlights the fundamental role of the plastid translation machinery during the early stages of embryo development in plants and raises the possibility of additional roles of plastid ribosomal proteins in the nucleus.</p>","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"35 3","pages":"189-204"},"PeriodicalIF":3.4,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9352626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10713837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Let's shape again: the concerted molecular action that builds the pollen tube.","authors":"Aslıhan Çetinbaş-Genç,&nbsp;Veronica Conti,&nbsp;Giampiero Cai","doi":"10.1007/s00497-022-00437-4","DOIUrl":"https://doi.org/10.1007/s00497-022-00437-4","url":null,"abstract":"<p><strong>Key message: </strong>The pollen tube is being subjected to control by a complex network of communication that regulates its shape and the misfunction of a single component causes specific deformations. In flowering plants, the pollen tube is a tubular extension of the pollen grain required for successful sexual reproduction. Indeed, maintaining the unique shape of the pollen tube is essential for the pollen tube to approach the embryo sac. Many processes and molecules (such as GTPase activity, phosphoinositides, Ca²⁺ gradient, distribution of reactive oxygen species and nitric oxide, nonuniform pH values, organization of the cytoskeleton, balance between exocytosis and endocytosis, and cell wall structure) play key and coordinated roles in maintaining the cylindrical shape of pollen tubes. In addition, the above factors must also interact with each other so that the cell shape is maintained while the pollen tube follows chemical signals in the pistil that guide it to the embryo sac. Any intrinsic changes (such as erroneous signals) or extrinsic changes (such as environmental stresses) can affect the above factors and thus fertilization by altering the tube morphology. In this review, the processes and molecules that enable the development and maintenance of the unique shape of pollen tubes in angiosperms are presented emphasizing their interaction with specific tube shape. Thus, the purpose of the review is to investigate whether specific deformations in pollen tubes can help us to better understand the mechanism underlying pollen tube shape.</p>","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"35 2","pages":"77-103"},"PeriodicalIF":3.4,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39828926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}