Plant Reproduction最新文献

{"title":"BONOBO transcription factors positively regulate STICKY GENERATIVE CELL for generative cell internalisation in Arabidopsis.","authors":"Sung-Aeong Oh, Soon Ki Park","doi":"10.1007/s00497-025-00527-z","DOIUrl":"https://doi.org/10.1007/s00497-025-00527-z","url":null,"abstract":"<p><strong>Key message: </strong>Generative cell internalisation establishes distinctive 'cell-within-a-cell' pollen organization in angiosperms. Comparative and transgenic expression analyses provide valuable insights into the cytological and genetic regulation underlying generative cell internalisation in Arabidopsis. For double fertilisation in flowering plants, a three-celled male gametophyte (pollen grain) delivers two non-motile sperm cells to the female gametes through a rapidly elongating pollen tube. This process is facilitated by a distinctive'cell-within-a-cell' pollen organisation, where the smaller generative cell becomes internalised into the larger vegetative cytoplasm through precisely regulated morphogenetic process and callose dynamics. However, the genetic mechanisms underlying generative cell internalisation remain unclear. Therefore, this study aims to comparatively analyse Arabidopsis wild-type with two different mutants that exhibit immobilised generative cells from the loss-of-function of DUF707 STICKY GENERATIVE CELL (SGC) and bHLH transcription factors BONOBO (BNB1) and BNB2, respectively. The results show that wild-type generative cells internalise through four successive steps. In contrast, bnb1;bnb2 mutants fail to progress to step 3 due to incomplete dissolution of the hemispherical callose and sgc-1 mutant proceeds to step 3 but arrests before step 4 with the intine-side callose dissolution disturbed. Moreover, transgenic analyses employing SGC promoter reporters and various BNB expression constructs revealed that SGC expression is positively regulated by BNB transcription factors. These findings enhance the understanding of the cytological and genetic regulation involving BNB transcription factors and downstream components, including SGC, during generative cell internalisation.</p>","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"38 3","pages":"17"},"PeriodicalIF":2.9,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144621056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Semi-automated high content analysis of pollen performance using tubetracker.","authors":"Sorel V Yimga Ouonkap, Yahir Oseguera, Bryce Okihiro, Mark A Johnson","doi":"10.1007/s00497-025-00526-0","DOIUrl":"https://doi.org/10.1007/s00497-025-00526-0","url":null,"abstract":"<p><strong>Key message: </strong>TubeTracker provides a method to partially automate analysis of pollen tube growth using live imaging. Pollen function is critical for successful plant reproduction and crop productivity and it is important to develop accessible methods to quantitatively analyze pollen performance to enhance reproductive resilience. Here we introduce TubeTracker as a method to quantify key parameters of pollen performance such as, time to pollen grain germination, pollen tube tip velocity and maintenance of pollen tube integrity. TubeTracker integrates manual and automatic image processing routines and the graphical interface allows the user to interact with the software to make manual corrections of automated steps. TubeTracker does not depend on training data sets required to implement machine learning approaches and thus can be immediately implemented using readily available imaging systems. Furthermore, TubeTracker is an excellent tool to produce the pollen performance data sets necessary to take advantage of emerging AI-based methods to fully automate analysis. We tested TubeTracker and found it to be accurate in measuring pollen tube germination and pollen tube tip elongation across multiple cultivars of tomato.</p>","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"38 3","pages":"16"},"PeriodicalIF":2.9,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144303550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The regulation of vacuole morphology in stigma papilla cells is involved in water transfer to pollen in Arabidopsis thaliana.","authors":"Kazuki Fukushima, Maki Hayashi, Masao Watanabe","doi":"10.1007/s00497-025-00525-1","DOIUrl":"10.1007/s00497-025-00525-1","url":null,"abstract":"<p><strong>Key message: </strong>The stigma papilla cells of Arabidopsis thaliana control water transport to pollen by regulating the morphology of vacuoles in papilla cells after pollination. Pollen hydration is the first crucial response after pollination for successful fertilization. In the Brassicaceae family, papilla cells on the stigma supply water to pollen. In pollinated papilla cells, cellular responses essential for pollen hydration are induced. However, it remains unclear how papilla cells release water from inside the cells to the pollen. Here, we set up a live-cell imaging system for observing vacuole dynamics in Arabidopsis thaliana papilla cells and investigated the role of vacuole morphology in these cells in the regulation of water transfer to pollen. Before pollination, vacuoles in the papilla cells changed their morphology through fusion and constriction; however, after pollination, they formed larger vacuoles and exhibited reduced movement. Additionally, when the morphological variation of vacuoles in the papilla cells was inhibited by wortmannin treatment, the pollen hydration rate decreased in a concentration-dependent manner. In contrast, the vacuoles tended to be less constricted even before pollination and showed less variation than wild-type after pollination in Rho-like GTPase from plants 2 (ROP2) mutant papilla cells, where the pollen hydration rate is faster. We propose that the regulation of vacuole morphology in papilla cells is involved in water transfer to pollen during pollination.</p>","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"38 2","pages":"15"},"PeriodicalIF":2.9,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12144065/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144235930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ROS regulation of stigma papillae growth and maturation in Arabidopsis thaliana.","authors":"Subramanian Sankaranarayanan, Sowmiya D Venkatesan, Thomas C Davis, Sharon A Kessler","doi":"10.1007/s00497-025-00524-2","DOIUrl":"10.1007/s00497-025-00524-2","url":null,"abstract":"<p><strong>Key message: </strong>Superoxide accumulates during early stigma papillae growth stages in Arabidopsis. Highly specialized stigma papillae cells play a critical role in plant reproduction. Their main purpose is to catch and interact with pollen, to mediate compatibility responses, to regulate pollen germination, and to guide pollen tubes to the transmitting tract so that the sperm cells carried in the pollen can be delivered to the female gametophyte to achieve double fertilization. In Arabidopsis thaliana, the stigma consists of single-celled stigma papillae that emerge from the apex of the fused carpels. Despite their critical function in plant reproduction, the molecular mechanisms that govern growth and maturation of stigma papillae remain poorly understood. Reactive Oxygen Species (ROS) have been implicated in stigma receptivity, but their roles in papillae development are less explored. Here we show that reactive oxygen species (ROS) also play different roles in stigma papillae development, with superoxide accumulating during the initiation and growth phase and hydrogen peroxide accumulating in mature papillae that are receptive to pollen. Reducing superoxide levels in the stigma by pharmacological treatments or over-expressing superoxide dismutase enzymes under an early stigma promoter inhibited stigma papillae growth, suggesting that ROS homeostasis is critical to papillae growth and differentiation for optimal pollination.</p>","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"38 2","pages":"14"},"PeriodicalIF":2.9,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12137503/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144227503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anisotropic diffuse growth in Arabidopsis thaliana stigma papillae.","authors":"Thomas C Davis, Sharon A Kessler","doi":"10.1007/s00497-025-00523-3","DOIUrl":"10.1007/s00497-025-00523-3","url":null,"abstract":"<p><strong>Key message: </strong>Arabidopsis stigma papillae grow by a diffuse growth mechanism rather than by tip growth. In angiosperms, the stigma is the first point of contact between the pollen (male) and pistil (female) during pollination. The stigma facilitates pollen capture and adhesion, compatibility responses, pollen germination, and pollen tube guidance to the transmitting tract. In Arabidopsis thaliana, the stigma is composed of single-celled stigma papillae that initiate from the apex of the carpels. Despite their critical function in plant reproduction, little is known about the cell and molecular mechanisms that govern stigma papillae growth and development. Using morphometric analysis of stigma papillae growth during different stages of floral development, we show that A. thaliana stigma papillae grow via a diffuse growth mechanism. Consistent with this conclusion, several mutants with reduced growth anisotropy in vegetative tissues due to defective cellulose or microtubule function likewise reduce anisotropy in stigma papillae.</p>","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"38 2","pages":"13"},"PeriodicalIF":2.9,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12137461/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sexual reproduction in land plants: an evolutionary perspective.","authors":"Annette Becker, Xia Chen, Thomas Dresselhaus, Nora Gutsche, Stefanie J Müller-Schüssele, Stefanie Sprunck, Günter Theißen, Sophie de Vries, Sabine Zachgo","doi":"10.1007/s00497-025-00522-4","DOIUrl":"10.1007/s00497-025-00522-4","url":null,"abstract":"<p><strong>Key message: </strong>We link key aspects of land plant reproductive evolution and detail how successive molecular changes leading to novel tissues and organs require co-evolution of communication systems between tissues. The transition of water-dependent reproduction of algae to mechanisms with very limited water dependence in many land plant lineages allowed plants to colonize diverse terrestrial environments, leading to the vast variety of extant plant species. The emergence of modified cell types, novel tissues, and organs enabled this transition; their origin is associated with the co-evolution of novel or adapted molecular communication systems and gene regulatory networks. In the light of an increasing number of genome sequences in combination with the establishment of novel genetic model organisms from diverse green plant lineages, our knowledge and understanding about the origin and evolution of individual traits that arose in a concerted way increases steadily. For example, novel members of gene families in signaling pathways emerged for communication between gametes and gametophytes with additional tissues surrounding the gametes. Here, we provide a comprehensive overview on the origin and evolution of reproductive novelties such as pollen grains, immobile sperms, ovules and seeds, carpels, gamete/gametophytic communication systems, double fertilization, and the molecular mechanisms that have arisen anew or have been co-opted during evolution, including but not limited to the incorporation of phytohormones, reactive oxygen species and redox signaling as well as small RNAs in regulatory modules that contributed to the evolution of land plant sexual reproduction.</p>","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"38 2","pages":"12"},"PeriodicalIF":2.9,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12069490/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Haustorial processes during the female gametophyte formation in Rosularia pallida (Schott & Kotschy) Stapf (Crassulaceae).","authors":"Emilia Brzezicka, Małgorzata Kozieradzka-Kiszkurno","doi":"10.1007/s00497-025-00521-5","DOIUrl":"10.1007/s00497-025-00521-5","url":null,"abstract":"<p><strong>Key message: </strong>Ultrastructural and cytochemical analyses of the megaspore, embryo sac, and synergid haustoria reveal their roles in nutrition, contributing to the successful development of the megagametophyte in R. pallida. In this paper, we present the first cytochemical and ultrastructural analysis of the megaspores, embryo sac, and synergid haustoria in Rosularia pallida (Schott & Kotschy) Stapf (Crassulaceae) are presented. The haustoria in the ovule of R. pallida primarily function to provide nutrition during megasporogenesis and megagametogenesis. Cytochemical staining reveals a significant increase in the accumulation of insoluble polysaccharides, lipids, and proteins within the megaspores and embryo sac. This increase occurs alongside the progressive degradation of nucellar cells and the growth of haustoria towards the integuments. The direction of haustorial growth within sporophyte tissues and the distribution of nutrients within the ovule complement each other, collectively contributing to efficient nutrition for the developing female gametophyte. Callose is present in the walls of both the megaspores and their haustoria. The functional megaspore (FM) haustorium is the only one that extends beyond the nucellus into the integuments during megasporogenesis. The disappearance of callose in the micropylar portion of the FM haustorium enables apoplasmic transport, particularly in this region. These findings suggest that the FM haustorium supports the development of a specific megaspore in the tetrad, indirectly influencing FM selection through nutrient provision. Furthermore, the removal of callose on the chalazal side of the tetrad likely facilitates the development of the embryo sac from the chalazal megaspore. Ultrastructural analyses of the megaspore, embryo sac, and synergid haustoria reveal the presence of transfer-wall ingrowths. No plasmodesmata were detected in the haustorial walls. Additionally, ultrastructural observations of the synergids indicate that their haustorium significantly elongates toward the micropyle and becomes metabolically active.</p>","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"38 2","pages":"11"},"PeriodicalIF":2.9,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143789417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential regulation of cleistogamy in pigeonpea through jasmonic acid and bHLH transcription factor interactions.","authors":"Palak Gupta, Harsha Srivastava, Kuldeep Kumar, Machindra Nirgude, Kumari Arpita, Jyothilakshmi Vadassery, Sandhya Sharma, Malik Zainul Abdin, Kishor Gaikwad","doi":"10.1007/s00497-025-00520-6","DOIUrl":"10.1007/s00497-025-00520-6","url":null,"abstract":"<p><strong>Key message: </strong>This study provides insights into the molecular and hormonal control of cleistogamy in pigeonpea, focusing on bHLH transcription factors and jasmonic acid pathway. Pigeonpea, an annual diploid (2n = 22) grain legume, holds significant nutritional value in cereal-dominated diets. The chasmogamous flowers of pigeonpea have a typical 9 + 1 diadelphous stamen where flowers open pre-fertilization resulting in cross-pollination. In contrast, a cleistogamous genotype characterized by polyadelphous stamens and flowers that open post-fertilization ensuring seed purity was analyzed for identifying causal pathways. Subsequent analysis focused on a set of transcription factors and their interaction with the hormonal networks associated with cleistogamy. Genes of the Jasmonic acid (JA) signaling pathway have been established to play a significant role in inducing cleistogamy and one of the key regulators of the JA pathway is bHLH (basic helix loop helix). A genome-wide survey identified 176 bHLH genes in the pigeonpea genome. Phylogenetic analysis classified 176 bHLH genes into 21 subfamilies distributed randomly across the genome. Gene ontology, cis-motifs analysis in the upstream region, and protein-protein interaction network implied the involvement of these genes in various biological processes. Expression analysis of key genes of the jasmonic acid pathway which includes MYC2 (Cc_bHLH135) along with its interacting partners TIFY/JAZ in chasmogamous and cleistogamous floral tissues revealed their potential role in flower opening. The results of UHPLC-MS/MS quantitation of Jasmonic acid and its bioactive form JA-Ile align with the expression analysis. The congruence of gene expression and hormone profiling highlights the involvement of the JA pathway in regulating flower opening, implying their potential role in cleistogamy in pigeonpea.</p>","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"38 2","pages":"10"},"PeriodicalIF":2.9,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143722442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Providence 2024: meeting of the International Association of Sexual Plant Reproduction Research.","authors":"Naoya Sugi, Sarita Muñoz-Gómez, Daniel S Jones","doi":"10.1007/s00497-025-00519-z","DOIUrl":"10.1007/s00497-025-00519-z","url":null,"abstract":"","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"38 2","pages":"9"},"PeriodicalIF":2.9,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review on the function of arabinogalactan-proteins during pollen grain development.","authors":"Sara Foubert-Mendes, Jessy Silva, Maria João Ferreira, Luís Gustavo Pereira, Sílvia Coimbra","doi":"10.1007/s00497-024-00515-9","DOIUrl":"10.1007/s00497-024-00515-9","url":null,"abstract":"<p><strong>Key message: </strong>Overview of the current understanding of PG development, PT growth and the role of AGPs in these processes. The pollen grain (PG) is a complex structure composed of three cells: the vegetative cell which develops into a pollen tube (PT) and two sperm cells that will fuse with the egg cell and central cell, giving rise to the embryo and endosperm, respectively. This resilient gametophyte is constantly subjected to selective pressures, leading to a diverse range of characteristics, with one of its defining features being the pollen cell wall. In this review, we closely examine the developmental stages of PG formation and PT growth, with a specific focus on the dynamic roles of arabinogalactan-proteins (AGPs) throughout these processes. AGPs are initially present in pollen mother cells and persist throughout PT growth. In the early stages, AGPs play a crucial role in primexine anchoring, followed by nexine and intine formation as well as cellulose deposition, thereby providing essential structural support to the PG. As PGs mature, AGPs continue to be essential, as their absence often leads to the collapse of PGs before they reach full maturity. Moreover, the absence of AGPs during PT growth leads to abnormal growth patterns, likely due to disruptions of cellulose, callose, and F-actin deposition, as well as perturbations in calcium ion (Ca²⁺) signalling. Understanding the intricate interplay between AGPs and PG development sheds light on the underlying mechanisms that drive reproductive success and highlights the indispensable role of AGPs in ensuring the integrity and functionality of PGs.</p>","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"38 1","pages":"8"},"PeriodicalIF":2.9,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11802600/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143257345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}