Plant Reproduction最新文献

SDJ, a pollen-expressed type III J-protein in Prunus, directly binds S-RNase and promotes recruitment of SLFL6 to an S-RNase-associated complex. SDJ是李属花粉表达的III型j蛋白，直接结合S-RNase并促进SLFL6募集到S-RNase相关复合物。

IF 2.6 4区生物学

Plant Reproduction Pub Date : 2026-08-29 DOI: 10.1007/s00497-026-00545-5

Xuexi Dou, Daiki Matsumoto, Soichiro Nishiyama, Ryutaro Tao

{"title":"SDJ, a pollen-expressed type III J-protein in Prunus, directly binds S-RNase and promotes recruitment of SLFL6 to an S-RNase-associated complex.","authors":"Xuexi Dou, Daiki Matsumoto, Soichiro Nishiyama, Ryutaro Tao","doi":"10.1007/s00497-026-00545-5","DOIUrl":"https://doi.org/10.1007/s00497-026-00545-5","url":null,"abstract":"In Prunus, self-incompatibility (SI) is controlled by S-RNases and pollen-expressed F-box proteins, whereas the molecular processes governing S-RNase regulation in pollen remain incompletely understood. Here, we characterized PavSDJ, a novel pollen protein from sweet cherry (Prunus avium), as a candidate factor involved in pollen-side S-RNase-associated processes. Sequence and structural analyses identified PavSDJ as a type III J-protein. Phylogenetic analyses placed PavSDJ within a distinct SDJ-like sublineage of the type III J-protein group, separate from a closely related sister lineage. Consistent with this divergence, PavSDJ was strongly expressed in anthers and pollen, whereas its sister gene was broadly expressed across organs. Transient expression assays showed that PavSDJ-GFP exhibited a predominantly cell-peripheral fluorescence pattern consistent with intracellular localization. Biochemical analyses showed that PavSDJ associated with recombinant PavS-RNases in pollen extracts and in reconstituted pull-down assays, without obvious allele preference. Proteomic analysis of PavSDJ co-immunoprecipitants from pollen extracts identified a complex including PavSLFL6 and PavSSK1. Reconstitution assays further showed that PavSDJ promoted the co-precipitation of PavSLFL6 with S-RNase. These findings identify PavSDJ as a candidate pollen-side factor in the Prunus SI pathway and provide evidence that a specialized J-protein may contribute to SI-related protein complex assembly.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"39 3-4","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148857880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The SbTDR gene is required for anther development and male fertility in Sorghum bicolor. SbTDR基因是双色高粱花药发育和雄性育性的必需基因。

IF 2.6 4区生物学

Plant Reproduction Pub Date : 2026-08-25 DOI: 10.1007/s00497-026-00546-4

Ashley R Smith, Jian Huang, Uyiosasere D Aigbe, Xia Wan, Junping Chen, Zhanguo Xin, Dazhong Zhao

{"title":"The SbTDR gene is required for anther development and male fertility in Sorghum bicolor.","authors":"Ashley R Smith, Jian Huang, Uyiosasere D Aigbe, Xia Wan, Junping Chen, Zhanguo Xin, Dazhong Zhao","doi":"10.1007/s00497-026-00546-4","DOIUrl":"10.1007/s00497-026-00546-4","url":null,"abstract":"Anthers are essential in plant reproduction and are also crucial in agriculture, because hybrid seed production typically relies on the manipulation of male fertility. Sorghum (Sorghum bicolor L. Moench) is the fifth most produced grain crop in the world. However, relatively little is known about the genetic mechanisms that regulate anther development and male fertility in sorghum. Here, we report the morphological characterization of anther development in wild-type sorghum BTx623 and the Sorghum bicolor tapetal degeneration retardation (Sbtdr) mutant, as well as identification of the causal gene SbTDR. Our results showed that the Sbtdr mutant formed small, white anthers that failed to produce pollen grains. We classified sorghum anther development into 13 main stages based on major cellular landmarks observed in semi-thin sections of anthers. Our analysis demonstrated that the Sbtdr mutant is defective in tapetal cell degeneration. Moreover, in situ hybridization revealed that the SbTDR gene, which encodes a bHLH transcription factor, is primarily expressed in tapetal cells at the microspore stage. Our discoveries highlight the importance of the SbTDR bHLH transcription factor in controlling tapetal cell differentiation and male sterility, which may help develop novel hybrid breeding systems in sorghum.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"39 3-4","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13506635/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148820178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Close contact: role of the extracellular matrix in cell adhesion and separation during plant development and reproduction. 密切接触：在植物发育和繁殖过程中，细胞外基质在细胞粘附和分离中的作用。

IF 2.6 4区生物学

Plant Reproduction Pub Date : 2026-07-30 DOI: 10.1007/s00497-026-00544-6

Juliette Cremer, Marc Ropitaux, Arnaud Lehner, Jean-Claude Mollet

{"title":"Close contact: role of the extracellular matrix in cell adhesion and separation during plant development and reproduction.","authors":"Juliette Cremer, Marc Ropitaux, Arnaud Lehner, Jean-Claude Mollet","doi":"10.1007/s00497-026-00544-6","DOIUrl":"https://doi.org/10.1007/s00497-026-00544-6","url":null,"abstract":"In plant organisms, cells have two ways of being connected to each other. They are either adhering to the same neighbouring cells since mitosis or they enter in contact de novo with a new cell and adhered themselves. These different intercellular interactions have multiple roles depending on the location of the cell studied or the stage of the plant's life cycle. Intercellular adhesion, for example, helps in maintaining tissue integrity, controlling cell shape, and even plays a role in defense. De novo cell adhesion, on the other hand, occurs at specific times in the life of the plant, particularly at three stages: cell elongation of fibres and their adhesion to each other, and during sexual plant reproduction: adhesion of the pollen grain to the stigma and adhesion of the pollen tube to the transmitting tract cells in the style and ovary. It is already well-known that the cell wall regulates cellular interactions and governs intercellular and de novo cell adhesion but only few studies have focused specifically on the molecules involved in the second mechanisms. In this review, we summarize current knowledge on the polymers involved in cell adhesion and separation in sporophyte tissues and male gametophytes on/in the female sporophyte.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"39 3-4","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148622290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PbrFEN-1 positively regulates pear pollen tube growth and is involved in the self-incompatibility response. PbrFEN-1正调控梨花粉管生长，参与自交不亲和反应。

IF 2.6 4区生物学

Plant Reproduction Pub Date : 2026-07-09 DOI: 10.1007/s00497-026-00541-9

Zhi-Heng Sui, Yi-Peng Ye, Ju-Yang Yao, Ya-Ru Wang, Ying Xu, Ce-Xian Cui, Shao-Ling Zhang, Chao Gu

{"title":"PbrFEN-1 positively regulates pear pollen tube growth and is involved in the self-incompatibility response.","authors":"Zhi-Heng Sui, Yi-Peng Ye, Ju-Yang Yao, Ya-Ru Wang, Ying Xu, Ce-Xian Cui, Shao-Ling Zhang, Chao Gu","doi":"10.1007/s00497-026-00541-9","DOIUrl":"https://doi.org/10.1007/s00497-026-00541-9","url":null,"abstract":"S-RNase-mediated gametophytic self-incompatibility (GSI) prevents self‑fertilization by inhibiting the growth of self-pollen tubes, yet the contribution of DNA metabolism, particularly radiation sensitive 2 (RAD2) family nucleases, to this process remains largely unknown. In this study, 49 RAD2 family genes were identified across pear and eight other fruit crops and classified into four conserved subfamilies (XPG, FEN-1, EXO1, and SEND). Of these, six RAD2 members were identified in pear, among which only PbrFEN-1 was significantly downregulated in pollen tubes treated with self S-RNases compared to non-self S-RNases, Furthermore, overexpression of PbrFEN-1 was found to alleviate the toxic effect of self S-RNase, indicating its involvement in the GSI response. Notably, antisense oligonucleotide-mediated knockdown of PbrFEN-1 disrupted ROS homeostasis at the pollen tube tip, leading to reduced ROS levels and subsequent inhibition of pollen tube growth. In contrast, pollen magnetofection-mediated overexpression of PbrFEN-1 helped maintain ROS homeostasis at the pollen tube tip, which was associated with faster pollen tube growth. These results suggest that PbrFEN-1 positively regulates ROS levels at the tip of pollen tube to support pollen tube elongation. These findings extend the mechanistic understanding of S-RNase-mediated GSI to the level of DNA metabolism and genome stability, providing new insights into the molecular basis of pollen tube inhibition in Rosaceae species.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"39 3-4","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148413724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Apomixis frequency in polyploid wild populations of Psidium cattleyanum f. lucidum (Myrteae, Myrtaceae). 桃金娘科（桃金娘科）桃金娘多倍体野生居群无杂交频率的研究。

IF 2.6 4区生物学

Plant Reproduction Pub Date : 2026-07-04 DOI: 10.1007/s00497-026-00543-7

Mercedes Souza-Pérez, Raquel Machado, Magdalena Vaio, Alejandra Borges, José I Hormaza, Gabriela Speroni

{"title":"Apomixis frequency in polyploid wild populations of Psidium cattleyanum f. lucidum (Myrteae, Myrtaceae).","authors":"Mercedes Souza-Pérez, Raquel Machado, Magdalena Vaio, Alejandra Borges, José I Hormaza, Gabriela Speroni","doi":"10.1007/s00497-026-00543-7","DOIUrl":"https://doi.org/10.1007/s00497-026-00543-7","url":null,"abstract":"The link between apomictic reproduction and polyploidy in plants has generated numerous hypotheses. Psidium cattleyanum is a valuable model for investigating this relationship, as it is a polyploid woody species that exhibits pseudogamous gametophytic apomixis. In this study, we aim to determine the predominant reproductive mode in natural populations of Psidium cattleyanum f. lucidum. We assessed its association with ploidy level, genetic diversity, and population structure. We analyzed populations representing the four ploidy levels (2C = 5x, 6x, 7x, 8x) present in Uruguay. Reproductive pathways were inferred by combining flow cytometric seed screening (FCSS) with microsatellite (SSR) genotyping of progeny arrays, and population-level differences were evaluated across both datasets. Diplosporous pseudogamous apomixis was identified as the predominant reproductive mode across populations. Importantly, the relative contributions of apomictic versus sexual reproduction as well as the frequencies of different seed progeny types varied markedly among populations and ploidy levels. Genotyping revealed predominantly clonal seed progeny, yet high within-population genetic variability and strong genetic structure were maintained. Moreover, gamete ploidy levels and their combinations were characteristic of each population, resulting in distinct patterns of seed formation among populations. Overall, facultative pseudogamous apomixis predominates in natural populations of P.c. f. lucidum. Our findings demonstrate previously unrecognized variation in reproductive pathways among populations, linked to differences in ploidy level and population genetic structure.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"39 3-4","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148388004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological basis for the development of pistillate and staminate florets in a dioecious species of Poa (Poeae, Pooideae, Poaceae). 蒲公英（蒲公英科，蒲公英科）雌雄异株雌蕊和雄蕊小花发育的形态学基础。

IF 2.6 4区生物学

Plant Reproduction Pub Date : 2026-06-12 DOI: 10.1007/s00497-026-00542-8

Juca A B San Martin, Abelardo C Vegetti, Raúl E Pozner, Nora G Uberti Manassero, Carlos A Dezar, Liliana M Giussani, Andrea G Reutemann

{"title":"Morphological basis for the development of pistillate and staminate florets in a dioecious species of Poa (Poeae, Pooideae, Poaceae).","authors":"Juca A B San Martin, Abelardo C Vegetti, Raúl E Pozner, Nora G Uberti Manassero, Carlos A Dezar, Liliana M Giussani, Andrea G Reutemann","doi":"10.1007/s00497-026-00542-8","DOIUrl":"10.1007/s00497-026-00542-8","url":null,"abstract":"The formation of unisexual florets in Poaceae occurs through diverse developmental pathways. However, the morphogenetic mechanisms controlling sex determination in dioecious grasses remain poorly understood. Here, we investigated the developmental basis of floral unisexuality in the dioecious forage species Poa lanigera (section Dioicopoa) to determine whether its developmental trajectory conforms to previously described patterns in grasses or represents a distinct pathway. We also compared stamen arrest in pistillate florets of P. lanigera with suppression of the third stamen in the model grass Brachypodium distachyon to assess potential similarities in organ arrest mechanisms. By using scanning electron microscopy and histological analyses, we reconstructed the sequence of floret organ initiation and differentiation in both species. Florets were initially established as perfect and followed a common sequence of organ initiation, with divergence arising shortly after ovary closure, stylar branch initiation, and the transition of anthers to the tetralobed stage. In pistillate florets of P. lanigera, androecium abortion involved early disorganization of hypodermal anther tissues, which prevented sporogenous differentiation and microsporogenesis, whereas, in staminate florets, gynoecium abortion occurred after sporogenous cell formation through localized tissue degeneration, which disrupted megasporogenesis. These developmental routes differ from previously described patterns in Poaceae, indicating a distinct variant of floral unisexuality. In contrast, suppression of the third stamen in B. distachyon occurred at the primordium stage without tissue degeneration, suggesting a mechanistically different process. Together, these findings provide a developmental framework to investigate the genetic regulation of sex determination in P. lanigera, with potential applications for pasture improvement.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"39 3-4","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148228815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Basis of apomixis in flowering plants - state of the art and research perspectives. 开花植物无融合的基础——研究现状与展望。

IF 2.6 4区生物学

Plant Reproduction Pub Date : 2026-05-30 DOI: 10.1007/s00497-026-00540-w

Agnieszka Barbara Springer, Krystyna Musiał

{"title":"Basis of apomixis in flowering plants - state of the art and research perspectives.","authors":"Agnieszka Barbara Springer, Krystyna Musiał","doi":"10.1007/s00497-026-00540-w","DOIUrl":"10.1007/s00497-026-00540-w","url":null,"abstract":"In most angiosperm plants, seeds are produced through a sexual process. Some angiosperms have developed an alternative reproductive strategy termed apomixis which involves producing seeds without the key steps of sexual development (i.e. meiosis, fertilization) and results in progeny genetically identical to the mother plant. Despite significant research advances, the evolutionary origin and molecular nature of apomixis remain unclear. Recent findings suggest that apomixis may have emerged from a sexual pathway deregulated by genetic and epigenetic modifications and stress signals (both endogenous and exogenous) that led to the alteration or omission of selected stages of sexual development. Apomixis, as a natural phenomenon that enables clonal reproduction by seeds, is a desirable trait with great potential in plant breeding, especially in terms of preserving hybrid vigor and gene combinations of elite phenotypes over subsequent generations. Notwithstanding, apomixis does not occur in major crops and therefore research programs focus on how to introgress, induce, or mimic apomixis in agronomically significant sexual species. The present review outlines the history of research on apomixis in flowering plants, its mechanisms, and a summary of the latest and most impactful research advances that may pave the way for the introduction of apomixis to sexually reproducing crops. The reader's attention is also drawn to the partially explained issue of intercellular communication in ovules during early apomictic processes in the context of the cell wall's changed chemical composition in cells that enter the apomictic developmental pathway.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"39 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13221432/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148058173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SWI3A/B regulates the transition from vegetative to reproductive phase in the liverwort Marchantia polymorpha. SWI3A/B调控地茅从营养阶段到生殖阶段的转变。

IF 2.6 4区生物学

Plant Reproduction Pub Date : 2026-05-20 DOI: 10.1007/s00497-026-00537-5

Maria Lozano-Quiles, Parth K Raval, Stefan A Rensing, Sven B Gould

{"title":"SWI3A/B regulates the transition from vegetative to reproductive phase in the liverwort Marchantia polymorpha.","authors":"Maria Lozano-Quiles, Parth K Raval, Stefan A Rensing, Sven B Gould","doi":"10.1007/s00497-026-00537-5","DOIUrl":"10.1007/s00497-026-00537-5","url":null,"abstract":"Land plants alternate between multicellular haploid and diploid phases, requiring a tight coordination between vegetative growth and sexual reproduction. We investigated SWI3A/B, an ancient core subunit of the SWI/SNF complex, of the non-vascular liverwort Marchantia polymorpha. A mutation in the promoter of MpSWI3A/B affected gametangiophore development and spermiogenesis in males, revealing its male-specific role in reproductive development. The MpSWIA/B mutant line amplifies vegetative propagation in males under conditions that would normally induce reproductive growth. The phenotype was underpinned by transcriptomic changes, showing that MpSWI3A/B modulates key regulators of gametangiophore initiation (e.g. BONOBO, GLID), sperm development and motility (e.g. DUO1, PKAR), and asexual reproduction (e.g. KAI2). We propose that, as a chromatin-level regulator, MpSWI3A/B may contribute to balancing vegetative and reproductive phases and highlight the protein's potential when exploring ancient epigenetic functions that coordinate developmental phase transitions in land plants.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"39 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13190419/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147977059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of TERMINAL FLOWER 1 homologs from Macroptilium (Benth.) Urb. 菝葜（Benth.）末端花1同源物的鉴定市区。

IF 2.6 4区生物学

Plant Reproduction Pub Date : 2026-05-11 DOI: 10.1007/s00497-026-00538-4

Yemina Rocío Abelendo, Nicolás Arizpe David, Andrea Gabriela Sarmiento, Juan Marcelo Zabala, Karina Fabiana Ribichich

{"title":"Characterization of TERMINAL FLOWER 1 homologs from Macroptilium (Benth.) Urb.","authors":"Yemina Rocío Abelendo, Nicolás Arizpe David, Andrea Gabriela Sarmiento, Juan Marcelo Zabala, Karina Fabiana Ribichich","doi":"10.1007/s00497-026-00538-4","DOIUrl":"10.1007/s00497-026-00538-4","url":null,"abstract":"In subtropical rangelands of America, the grasses dominate the landscape, but native leguminous plants could be source of nutritional quality as well as benefit suppliers of environment adaptations. Species of Macroptilium have been included in forage improvement programs and characterization of genes involved in plant architecture could contribute to its improvement. TFL1 is responsible for repressing the onset of flowering, sustaining the indeterminate growth and conditioning the complete plant structure. In this study, we report the cloning of candidate genes for TFL1 functional homology from Macroptilium lathyroides (MlTFL1) and M. erythroloma (MeTFL1) and their sequences and phylogenetic analyses. The genes structure was conserved, the deduced amino acid sequences showed also conservation of key residues and structures for function, and the phylogenetic reconstructions resolved them as a sister clade of Phaseolus spp. and near to Vigna spp. sequences. We also described the functional characterization of MlTFL1 through assays of Arabidopsis tfl1 mutant complementation with severe tfl1-1/tfl1-1 or mild tfl1-11/tfl1-11 genotypes. In both mutant backgrounds, the expression of MlTFL1 rescued the determinate growth, the early flowering time, the reduction in number of rosette leaves and the increase of rosette branches of mutants. Besides, MlTFL1 accomplished intermediate compensation of the height reduction. By PCA analyses of traits, longer vegetative phase and more rosette leaves associated to less rosette branches. Moreover, more height and more cauline branches had less weight in their associations with the other three variables. We consider that MlTFL1 of Macroptilium lathyroides behave as a TFL1 functional homolog and its characterization could contribute to increase the knowledge of this species looking to the improvement of this native forage leguminous plant.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"39 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147870299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low-temperature stress modulates pollen tube growth through temperature-dependent multi-level regulatory mechanisms in Camellia sinensis. 低温胁迫通过温度依赖的多层次调控机制调节茶树花粉管生长。

IF 2.6 4区生物学

Plant Reproduction Pub Date : 2026-05-08 DOI: 10.1007/s00497-026-00539-3

Sena Acar, Aslıhan Çetinbaş-Genç

{"title":"Low-temperature stress modulates pollen tube growth through temperature-dependent multi-level regulatory mechanisms in Camellia sinensis.","authors":"Sena Acar, Aslıhan Çetinbaş-Genç","doi":"10.1007/s00497-026-00539-3","DOIUrl":"10.1007/s00497-026-00539-3","url":null,"abstract":"Low temperature is a major environmental stress factor that limits male reproductive success in plants; however, the mechanistic basis of pollen responses to different degrees of low temperature stress remains poorly understood. In this study, we investigated the cytological, biochemical, and molecular responses of tea (Camellia sinensis) pollen grains to low-temperature stress by germinating pollen in vitro at 15, 10, and 5 °C. Although pollen germination rate and pollen tube length were reduced under all low-temperature treatments compared with the control, the underlying regulatory responses differed significantly with the severity of low-temperature stress. At 15 °C, decreases in non-enzymatic antioxidants and stress-related proteins indicated an overall metabolic weakening, while the concomitant accumulation of callose, cellulose, and methyl-esterified pectins reflected an early structural adjustment of the pollen tube cell wall. At 10 °C, the increasing stress load was partially counterbalanced by the induction of enzymatic antioxidant activities, and continued cell wall reinforcement identified this temperature as a transitional state between metabolic limitation and coordinated defense activation. In contrast, exposure to 5 °C resulted in pronounced metabolic suppression, together with a shift of stress-related proteins toward membrane fractions and enhanced deposition of callose, cellulose, and particularly de-esterified acidic pectins, leading to increased cell wall rigidity and mechanical restriction of pollen tube elongation. Overall, this study demonstrates that low-temperature stress constrains pollen tube growth not through a single limiting factor but via a temperature-dependent reorganization of interconnected cytological, biochemical, and molecular mechanisms.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"39 2","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13156120/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147845660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0