Plant Reproduction最新文献_第2页

Localization and activity of lipoxygenase in the ovule of Larix kaempferi (Lamb.) Carr. during female gametophyte maturation. Larix kaempferi (Lamb.) Carr.雌配子体成熟过程中胚珠中脂氧合酶的定位和活性。

IF 2.9 4区生物学

Plant Reproduction Pub Date : 2024-12-01 Epub Date: 2024-07-26 DOI: 10.1007/s00497-024-00507-9

Aleksandra Seta-Koselska, Ewa Szczuka, Mateusz Koselski

{"title":"Localization and activity of lipoxygenase in the ovule of Larix kaempferi (Lamb.) Carr. during female gametophyte maturation.","authors":"Aleksandra Seta-Koselska, Ewa Szczuka, Mateusz Koselski","doi":"10.1007/s00497-024-00507-9","DOIUrl":"10.1007/s00497-024-00507-9","url":null,"abstract":"Key message: Lipoxygenase activity and localization vary throughout the development of Larix kaempferi ovules, with the highest enzyme activity observed in ovules at the cellular stage and the most intense immunogold reaction noted at the mature archegonium stage of gametophyte development. Lipoxygenases are a family of oxidoreductases with a significant role in biological systems, widespread in living organisms e.g. mammals, fish, corals, plants, mosses, algae, fungi, yeasts, and bacteria. Lipoxygenase activity in plants leads to the formation of phytooxylipins, i.e. signaling molecules, which play a crucial role in many significant physiological processes such as male and female gametophyte maturation, germination and seedling growth, pathogen resistance, abiotic stress response, fruit ripening, and senescence. The activity and localization of lipoxygenase change during plant growth and development. The localization of lipoxygenase in a developing ovule of Larix kaempferi was analyzed using the immunogold labeling method, and the activity was determined spectrophotometrically with linolenic acid as a substrate. Among the investigated stages, the immunogold reaction was the most intense at the mature archegonium stage in the ovule. Lipoxygenase was found in all parts of the L. kaempferi ovule. The largest number of immunogold particles was detected in the integument cells of all the analyzed stages of ovule development. Only one isoform of lipoxygenase with an optimum at pH 8 was active in the ovules during female gametophyte maturation. The highest enzyme activity was determined at the cellular stage, whereas the mature archegonium stage was characterized by its lowest level, which means that LOX activity in developing ovules of the Japanese larch is not correlated with the number of antibody-labeled molecules of the enzyme.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":" ","pages":"507-520"},"PeriodicalIF":2.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11511710/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141768006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hormonal influence on maize inflorescence development and reproduction. 激素对玉米花序发育和繁殖的影响

IF 2.9 4区生物学

Plant Reproduction Pub Date : 2024-12-01 Epub Date: 2024-10-05 DOI: 10.1007/s00497-024-00510-0

Amina Chaudhry, Zongliang Chen, Andrea Gallavotti

引用次数: 0

Application of genome editing in plant reproductive biology: recent advances and challenges. 基因组编辑在植物生殖生物学中的应用：最新进展与挑战。

IF 2.9 4区生物学

Plant Reproduction Pub Date : 2024-12-01 Epub Date: 2024-07-02 DOI: 10.1007/s00497-024-00506-w

Nilesh D Gawande, Hemal Bhalla, Anshul Watts, Rahul Mahadev Shelake, Subramanian Sankaranarayanan

{"title":"Application of genome editing in plant reproductive biology: recent advances and challenges.","authors":"Nilesh D Gawande, Hemal Bhalla, Anshul Watts, Rahul Mahadev Shelake, Subramanian Sankaranarayanan","doi":"10.1007/s00497-024-00506-w","DOIUrl":"10.1007/s00497-024-00506-w","url":null,"abstract":"Key message: This comprehensive review underscores the application of genome editing in plant reproductive biology, including recent advances and challenges associated with it. Genome editing (GE) is a powerful technology that has the potential to accelerate crop improvement by enabling efficient, precise, and rapid engineering of plant genomes. Over the last decade, this technology has rapidly evolved from the use of meganucleases (homing endonucleases), zinc-finger nucleases, transcription activator-like effector nucleases to the use of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (CRISPR/Cas), which has emerged as a popular GE tool in recent times and has been extensively used in several organisms, including plants. GE has been successfully employed in several crops to improve plant reproductive traits. Improving crop reproductive traits is essential for crop yields and securing the world's food supplies. In this review, we discuss the application of GE in various aspects of plant reproductive biology, including its potential application in haploid induction, apomixis, parthenocarpy, development of male sterile lines, and the regulation of self-incompatibility. We also discuss current challenges and future prospects of this technology for crop improvement, focusing on plant reproduction.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":" ","pages":"441-462"},"PeriodicalIF":2.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141494230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The plant early recombinosome: a high security complex to break DNA during meiosis. 植物早期重组体：减数分裂过程中断裂 DNA 的高度安全复合体。

IF 2.9 4区生物学

Plant Reproduction Pub Date : 2024-12-01 Epub Date: 2024-09-27 DOI: 10.1007/s00497-024-00509-7

Nahid Rafiei, Arnaud Ronceret

{"title":"The plant early recombinosome: a high security complex to break DNA during meiosis.","authors":"Nahid Rafiei, Arnaud Ronceret","doi":"10.1007/s00497-024-00509-7","DOIUrl":"10.1007/s00497-024-00509-7","url":null,"abstract":"Key message: The formacion of numerous unpredictable DNA Double Strand Breaks (DSBs) on chromosomes iniciates meiotic recombination. In this perspective, we propose a 'multi-key lock' model to secure the risky but necesary breaks as well as a 'one per pair of cromatids' model for the topoisomerase-like early recombinosome. During meiosis, homologous chromosomes recombine at few sites of crossing-overs (COs) to ensure correct segregation. The initiation of meiotic recombination involves the formation of DNA double strand breaks (DSBs) during prophase I. Too many DSBs are dangerous for genome integrity: if these DSBs are not properly repaired, it could potentially lead to chromosomal fragmentation. Too few DSBs are also problematic: if the obligate CO cannot form between bivalents, catastrophic unequal segregation of univalents lead to the formation of sterile aneuploid spores. Research on the regulation of the formation of these necessary but risky DSBs has recently advanced in yeast, mammals and plants. DNA DSBs are created by the enzymatic activity of the early recombinosome, a topoisomerase-like complex containing SPO11. This opinion paper reviews recent insights on the regulation of the SPO11 cofactors necessary for the introduction of temporally and spatially controlled DSBs. We propose that a 'multi-key-lock' model for each subunit of the early recombinosome complex is required to secure the formation of DSBs. We also discuss the hypothetical implications that the established topoisomerase-like nature of the SPO11 core-complex can have in creating DSB in only one of the two replicated chromatids of early prophase I meiotic chromosomes. This hypothetical 'one per pair of chromatids' DSB formation model could optimize the faithful repair of the self-inflicted DSBs. Each DSB could use three potential intact homologous DNA sequences as repair template: one from the sister chromatid and the two others from the homologous chromosomes.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":" ","pages":"421-440"},"PeriodicalIF":2.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11511760/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142332027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pectin methylesterase activities in reproductive tissues of maize plants with different haplotypes of the Ga1 and Ga2 cross incompatibility systems. Ga1和Ga2杂交不亲和系统不同单倍型玉米植株生殖组织中果胶甲基酯酶的活性。

IF 2.9 4区生物学

Plant Reproduction Pub Date : 2024-12-01 Epub Date: 2024-05-03 DOI: 10.1007/s00497-024-00502-0

Amruta R Bapat, M Paul Scott

{"title":"Pectin methylesterase activities in reproductive tissues of maize plants with different haplotypes of the Ga1 and Ga2 cross incompatibility systems.","authors":"Amruta R Bapat, M Paul Scott","doi":"10.1007/s00497-024-00502-0","DOIUrl":"10.1007/s00497-024-00502-0","url":null,"abstract":"Key message: Total PME activity in reproductive tissues was related to haplotypes at maize cross incompatibility loci, suggesting that these loci function by controlling PME activity. In maize, the pollination outcome depends on the haplotypes of the interacting male gametophyte (germinated pollen) and female sporophyte (silk) at several cross-incompatibility loci. Functional alleles (-S haplotypes) of the cross-incompatibility loci Ga1 and Ga2, both encode two pectin methylesterases (PMEs), one that is expressed in silk and the other in pollen. We examined total PME activity in reproductive tissues containing functional and null haplotypes at the Ga1 or Ga2 loci. In pollinated silks, there was a correlation between total PME activity and the -S haplotype pollen in both Ga1 and Ga2 systems. We did not detect a significant relationship between PME activity and pollination outcome of either system. We re-examined previously reported active site amino acid substitutions in PMEs encoded by cross incompatibility loci. We observed that different active site substitutions are present in the pollen and silk PMEs of cross incompatibility loci and these differences are conserved across Ga1, Ga2 and Tcb-1. This work establishes a relationship between total PME activity and the haplotypes of the Ga1 locus in pollinated silks.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":" ","pages":"479-488"},"PeriodicalIF":2.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11511756/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140874003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrating single nuclei and bulk RNA sequencing in rice shoot apical meristems uncovers candidate early floral transition gene networks. 整合水稻茎尖分生组织的单核和大量RNA测序揭示了候选的早期花过渡基因网络。

IF 2.9 4区生物学

Plant Reproduction Pub Date : 2024-11-29 DOI: 10.1007/s00497-024-00514-w

Daniele Traversa, Giulio Vicentini, Paolo Korwin Krukowski, Lucio Conti, Matteo Chiara, Vittoria Brambilla

引用次数: 0

Twin embryo formation by induced parthenogenesis. 通过诱导孤雌生殖形成双胚胎。

IF 2.9 4区生物学

Plant Reproduction Pub Date : 2024-11-29 DOI: 10.1007/s00497-024-00512-y

Hannah Peha, Hui Ren, Debra Skinner, Venkatesan Sundaresan

{"title":"Twin embryo formation by induced parthenogenesis.","authors":"Hannah Peha, Hui Ren, Debra Skinner, Venkatesan Sundaresan","doi":"10.1007/s00497-024-00512-y","DOIUrl":"10.1007/s00497-024-00512-y","url":null,"abstract":"Key message: Induction of parthenogenesis (embryo formation from unfertilized egg cells) by embryogenic transcription factors is associated with twin formation at high frequencies, and involves two distinct mechanisms. Synthetic apomixis has been achieved through the induction of parthenogenesis by ectopic expression of the Baby Boom family of transcription factors. An associated phenomenon from this process is the formation of polyembryony including twin progeny at high frequencies, but the underlying mechanisms have not been explored. Here, we provide a brief description of the phenomenon, discuss potential mechanisms for twin formation in flowering plants, propose two possible models for their occurrence, and evaluate the available evidence from both dizygotic and monozygotic twins in relation to these models. The two proposed models are independent, but they can operate in combination. We conclude that both models are required to explain the types of twins and triplets that we and others have observed. These models provide future directions for basic research, as well as suggest possible approaches towards reducing polyembryony when incorporating synthetic apomixis into crop plants such as maize where twinning is not desirable.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"38 1","pages":"3"},"PeriodicalIF":2.9,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142752030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From lock and key to molecular diplomacy: understanding pollen recognition and discrimination in brassicaceae. 从锁和钥匙到分子外交：了解黄铜科植物的花粉识别和分辨能力。

IF 2.9 4区生物学

Plant Reproduction Pub Date : 2024-11-27 DOI: 10.1007/s00497-024-00511-z

Hemal Bhalla, Kumari Ankita, Kumar Abhinandan, Trivima Sharma, Subramanian Sankaranarayanan

{"title":"From lock and key to molecular diplomacy: understanding pollen recognition and discrimination in brassicaceae.","authors":"Hemal Bhalla, Kumari Ankita, Kumar Abhinandan, Trivima Sharma, Subramanian Sankaranarayanan","doi":"10.1007/s00497-024-00511-z","DOIUrl":"10.1007/s00497-024-00511-z","url":null,"abstract":"Key message: Hybridization barriers in Brassicaceae play a pivotal role in governing reproductive success and maintaining speciation. In this perspective, we highlight recent advances revealing the intricate molecular mechanisms and the interplay among key players governing these barriers. Recent studies have shed light on the molecular mechanisms that govern hybridization barriers in Brassicaceae. The interplay between pollen coat proteins, stigmatic receptors, and signaling peptides plays a crucial role in determining the success of pollination. At the core of this system, autocrine stigmatic RALF peptides (sRALF) maintain the stigmatic barrier by activating the FERONIA (FER) and ANJEA (ANJ) receptor complex, triggering the RAC/ROP-RBOHD pathway and subsequent reactive oxygen species (ROS) production. It is now established that incompatible pollen rejection is mediated by two parallel pathways: the FER-RAC/ROP-RBOHD pathway, which generates ROS, and the ARC1-mediated pathway, which degrades compatible factors required for pollen growth. Conversely, compatible pollen overcomes the stigmatic barrier through the action of pollen coat proteins (PCP-B) and paracrine pollen-derived RALF peptides (pRALF), which compete with autocrine sRALF for receptor binding, enabling successful pollen hydration and tube penetration. The \"lock-and-key\" mechanism involving sRALF and pRALF provides species-specific recognition of compatible pollen. These findings offer valuable insights into the molecular basis of hybridization barriers and open new possibilities for overcoming these barriers in interspecific and intergeneric crosses within Brassicaceae, with potential applications in plant breeding and crop improvement. Future research should focus on elucidating the evolutionary dynamics of these signaling pathways and exploring their manipulation for crop breeding purposes.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"38 1","pages":"2"},"PeriodicalIF":2.9,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142734242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellular mechanism of polarized auxin transport on fruit shape determination revealed by time-lapse live imaging. 通过延时实时成像揭示果实形状决定过程中极化辅助素运输的细胞机制。

IF 2.9 4区生物学

Plant Reproduction Pub Date : 2024-11-21 DOI: 10.1007/s00497-024-00513-x

Yao Zhang, Hao-Ran Sun, Zhi-Cheng Hu, Yang Dong

{"title":"Cellular mechanism of polarized auxin transport on fruit shape determination revealed by time-lapse live imaging.","authors":"Yao Zhang, Hao-Ran Sun, Zhi-Cheng Hu, Yang Dong","doi":"10.1007/s00497-024-00513-x","DOIUrl":"10.1007/s00497-024-00513-x","url":null,"abstract":"Key message: Polarized auxin transport regulates fruit shape determination by promoting anisotropic cell growth. Angiosperms produce organs with distinct shape resultant from adaptive evolution. Understanding the cellular basis underlying the development of plant organ has been a central topic in plant biology as it is key to unlock the mechanisms leading to the diversification of plants. Variations in the location of synthesis, polarized auxin transport (PAT) have been proposed to account for the development of diverse organ shapes, but the exact cellular mechanism has yet to be elucidated. The Capsella rubella develops a perfect heart-shaped fruit from an ovate shape gynoecium that is tightly linked to the localized auxin synthesis in the valve tips and provides a unique opportunity to address this question. In this study, we studied auxin movement in the fruits and the cellular effect of N-1-Naphthylphthalamic Acid (NPA) on the fruit shape determination by constructing the pCrPIN3:PIN3:GFP reporter and live-imaging. We found PAT in the valve epidermis is in congruent with fruit shape development and NPA treatment disrupts the heat-shaped fruit development mainly by repressing cell anisotropic growth with minor effect on division. As the Capsella fruit is unusually big in size, we also included a detailed step-by-step protocol on how to conduct live-imaging experiment. We further test the utility of this protocol by conducting a live-imaging analysis of the gynophore in Arachis hypogaea. Collectively, the results of this study elucidated the mechanism on how auxin signal was translated into instructions guiding cell growth during organ shape determination. In addition, the description of the detailed live-imaging protocol will encourage further studies of the cellular mechanisms underlying shape diversification in angiosperms.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"38 1","pages":"1"},"PeriodicalIF":2.9,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142683534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Arabidopsis KASH protein SINE3 is involved in male and female gametogenesis 拟南芥 KASH 蛋白 SINE3 参与雌雄配子的发生

IF 3.4 4区生物学

Plant Reproduction Pub Date : 2024-09-16 DOI: 10.1007/s00497-024-00508-8

Morgan Moser, Norman R. Groves, Iris Meier

{"title":"The Arabidopsis KASH protein SINE3 is involved in male and female gametogenesis","authors":"Morgan Moser, Norman R. Groves, Iris Meier","doi":"10.1007/s00497-024-00508-8","DOIUrl":"https://doi.org/10.1007/s00497-024-00508-8","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Key message</h3>The Arabidopsis KASH protein SINE3 is involved in male and female gametophyte development, likely affecting the first post-meiotic mitosis in both cases, and is required for full seed set.<h3 data-test=\"abstract-sub-heading\">Abstract</h3>Linker of nucleoskeleton and cytoskeleton (LINC) complexes are protein complexes spanning the inner and outer membranes of the nuclear envelope (NE) and are key players in nuclear movement and positioning. Through their roles in nuclear movement and cytoskeletal reorganization, plant LINC complexes affect processes as diverse as pollen tube rupture and stomatal development and function. KASH proteins are the outer nuclear membrane component of the LINC complex, with conserved C-termini but divergent N-terminal cytoplasmic domains. Of the known Arabidopsis KASH proteins, SUN-INTERACTING NUCLEAR ENVELOPE PROTEIN 3 (SINE3) has not been functionally characterized. Here, we show that SINE3 is expressed at all stages of male and female gametophyte development. It is located at the NE in male and female gametophytes. Loss of SINE3 results in a female-derived seed set defect, with sine3 mutant ovules arresting at stage FG1. Pollen viability is also significantly reduced, with microspores arresting prior to pollen mitosis I. In addition, sine3 mutants have a minor male meiosis defect, with some tetrads containing more than four spores. Together, these results demonstrate that the KASH protein SINE3 plays a crucial role in male and female gametophyte development, likely affecting the first post-meiotic nuclear division in both cases.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"21 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142258909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0