Plant Reproduction最新文献_第2页

Pollen viability, longevity, and function in angiosperms: key drivers and prospects for improvement. 被子植物花粉的活力、寿命和功能：关键驱动因素和改进前景。

IF 2.9 4区生物学

Plant Reproduction Pub Date : 2024-09-01 Epub Date: 2023-11-05 DOI: 10.1007/s00497-023-00484-5

Rasha Althiab-Almasaud, Eve Teyssier, Christian Chervin, Mark A Johnson, Jean-Claude Mollet

{"title":"Pollen viability, longevity, and function in angiosperms: key drivers and prospects for improvement.","authors":"Rasha Althiab-Almasaud, Eve Teyssier, Christian Chervin, Mark A Johnson, Jean-Claude Mollet","doi":"10.1007/s00497-023-00484-5","DOIUrl":"10.1007/s00497-023-00484-5","url":null,"abstract":"Pollen grains are central to sexual plant reproduction and their viability and longevity/storage are critical for plant physiology, ecology, plant breeding, and many plant product industries. Our goal is to present progress in assessing pollen viability/longevity along with recent advances in our understanding of the intrinsic and environmental factors that determine pollen performance: the capacity of the pollen grain to be stored, germinate, produce a pollen tube, and fertilize the ovule. We review current methods to measure pollen viability, with an eye toward advancing basic research and biotechnological applications. Importantly, we review recent advances in our understanding of how basic aspects of pollen/stigma development, pollen molecular composition, and intra- and intercellular signaling systems interact with the environment to determine pollen performance. Our goal is to point to key questions for future research, especially given that climate change will directly impact pollen viability/longevity. We find that the viability and longevity of pollen are highly sensitive to environmental conditions that affect complex interactions between maternal and paternal tissues and internal pollen physiological events. As pollen viability and longevity are critical factors for food security and adaptation to climate change, we highlight the need to develop further basic research for better understanding the complex molecular mechanisms that modulate pollen viability and applied research on developing new methods to maintain or improve pollen viability and longevity.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71488664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

"Resilience in crop reproduction for food security: introducing RECROP COST action". "作物再生产的复原力促进粮食安全：引入 RECROP COST 行动"。

IF 2.9 4区生物学

Plant Reproduction Pub Date : 2024-09-01 Epub Date: 2024-02-14 DOI: 10.1007/s00497-024-00497-8

Sotirios Fragkostefanakis, Michal Lieberman-Lazarovich, David Honys

引用次数: 0

The synthesis of papaya fruit flavor-related linalool was regulated by CpTPS18 and CpNAC56. 木瓜果实风味相关芳樟醇的合成受CpTPS18和CpNAC56调控。

IF 2.9 4区生物学

Plant Reproduction Pub Date : 2024-09-01 Epub Date: 2023-11-15 DOI: 10.1007/s00497-023-00486-3

Yuan Yao, Wenhui Fu, Yue Yu, Suyan Wan, Wenping Zhang, Ray Ming

{"title":"The synthesis of papaya fruit flavor-related linalool was regulated by CpTPS18 and CpNAC56.","authors":"Yuan Yao, Wenhui Fu, Yue Yu, Suyan Wan, Wenping Zhang, Ray Ming","doi":"10.1007/s00497-023-00486-3","DOIUrl":"10.1007/s00497-023-00486-3","url":null,"abstract":"Papaya is a tropical fruit crop renowned for its rich nutrition, particularly pro-vitamin A. Aroma substances are a major component of fruit quality. While extensive research has been conducted on papaya aroma, there has been a notable lack of in-depth research into a specific class of substances. To bridge this gap, our study focused on analyzing the aroma components of various papaya varieties and their biosynthesis pathways. We compared the volatile components of three papaya varieties with distinct flavors at various ripeness stages. A continuous accumulation of linalool, a volatile compound, in the 'AU9' fruit was detected as it matured. The linalool content reached 56% of the total volatile components upon full ripening. Notably, this percentage was significantly higher than that observed in the other two varieties, 'ZhongBai' and 'Malaysian 7', indicating that linalool serves as the primary component influencing the papaya's odor. Subsequently, we identified CpTPS18, a gene associated with linalool biosynthesis, and demonstrated its ability to catalyze linalool production from GPP and enhance its accumulation through overexpression in papaya fruits, both in vivo and in vitro. Based on transcriptomic analysis, it was predicted that CpMYB56 and CpNAC56 may transcriptionally activate the expression of CpTPS18. Subsequent yeast one-hybrid assay and dual luciferase analysis revealed that CpNAC56 activates the transcription of CpTPS18. Transient overexpression in vivo demonstrated that this gene could upregulate the expression of CpTPS18 and promote linalool accumulation. These results uncovered the primary volatile molecule responsible for papaya fruit odor and identified two major genes influencing its biosynthesis. The genomic resources and information obtained from this study will expedite papaya improvement for fruit quality.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"107592771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

JAGGER localization and function are dependent on GPI anchor addition. JAGGER 的定位和功能取决于 GPI 锚点的添加。

IF 2.9 4区生物学

Plant Reproduction Pub Date : 2024-09-01 Epub Date: 2024-01-31 DOI: 10.1007/s00497-024-00495-w

Raquel Figueiredo, Mónica Costa, Diana Moreira, Miguel Moreira, Jennifer Noble, Luís Gustavo Pereira, Paula Melo, Ravishankar Palanivelu, Sílvia Coimbra, Ana Marta Pereira

{"title":"JAGGER localization and function are dependent on GPI anchor addition.","authors":"Raquel Figueiredo, Mónica Costa, Diana Moreira, Miguel Moreira, Jennifer Noble, Luís Gustavo Pereira, Paula Melo, Ravishankar Palanivelu, Sílvia Coimbra, Ana Marta Pereira","doi":"10.1007/s00497-024-00495-w","DOIUrl":"10.1007/s00497-024-00495-w","url":null,"abstract":"Key message: GPI anchor addition is important for JAGGER localization and in vivo function. Loss of correct GPI anchor addition in JAGGER, negatively affects its localization and function. In flowering plants, successful double fertilization requires the correct delivery of two sperm cells to the female gametophyte inside the ovule. The delivery of a single pair of sperm cells is achieved by the entrance of a single pollen tube into one female gametophyte. To prevent polyspermy, Arabidopsis ovules avoid the attraction of multiple pollen tubes to one ovule-polytubey block. In Arabidopsis jagger mutants, a significant number of ovules attract more than one pollen tube to an ovule due to an impairment in synergid degeneration. JAGGER encodes a putative arabinogalactan protein which is predicted to be anchored to the plasma membrane by a glycosylphosphatidylinositol (GPI) anchor. Here, we show that JAGGER fused to citrine yellow fluorescent protein (JAGGER-cYFP) is functional and localizes mostly to the periphery of ovule integuments and transmitting tract cells. We further investigated the importance of GPI-anchor addition domains for JAGGER localization and function. Different JAGGER proteins with deletions in predicted ω-site regions and GPI attachment signal domain, expected to compromise the addition of the GPI anchor, led to disruption of JAGGER localization in the cell periphery. All JAGGER proteins with disrupted localization were also not able to rescue the polytubey phenotype, pointing to the importance of GPI-anchor addition to in vivo function of the JAGGER protein.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11377618/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139643318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Arabidopsis SNARE complex genes regulate the early stages of pollen-stigma interactions. 拟南芥SNARE复杂基因调控花粉-柱头相互作用的早期阶段。

IF 2.9 4区生物学

Plant Reproduction Pub Date : 2024-09-01 Epub Date: 2023-12-01 DOI: 10.1007/s00497-023-00488-1

Stuart R Macgregor, Paula K S Beronilla, Daphne R Goring

{"title":"The Arabidopsis SNARE complex genes regulate the early stages of pollen-stigma interactions.","authors":"Stuart R Macgregor, Paula K S Beronilla, Daphne R Goring","doi":"10.1007/s00497-023-00488-1","DOIUrl":"10.1007/s00497-023-00488-1","url":null,"abstract":"Key message: The VAMP721, VAMP722, SYP121, SYP122 and SNAP33 SNAREs are required in the Arabidopsis stigma for pollen hydration, further supporting a role for vesicle trafficking in the stigma's pollen responses. In the Brassicaceae, the process of accepting compatible pollen is a key step in successful reproduction and highly regulated following interactions between the pollen and the stigma. Central to this is the initiation of secretion in the stigma, which is proposed to provide resources to the pollen for hydration and germination and pollen tube growth. Previously, the eight exocyst subunit genes were shown to be required in the Arabidopsis stigma to support these pollen responses. One of the roles of the exocyst is to tether secretory vesicles at the plasma membrane for membrane fusion by the SNARE complex to enable vesicle cargo release. Here, we investigate the role of Arabidopsis SNARE genes in the stigma for pollen responses. Using a combination of different knockout and knockdown SNARE mutant lines, we show that VAMP721, VAMP722, SYP121, SYP122 and SNAP33 are involved in this process. Significant disruptions in pollen hydration were observed following pollination of wildtype pollen on the mutant SNARE stigmas. Overall, these results place the Arabidopsis SNARE complex as a contributor in the stigma for pollen responses and reaffirm the significance of secretion in the stigma to support the pollen-stigma interactions.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138464266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ectopic expression of a thaumatin-like protein impairs the timely deposition and dissolution of callose during microsporogenesis, leading to microspore death and male sterility in Arabidopsis. 异位表达一种haumatin样蛋白会影响小孢子发生过程中胼胝质的及时沉积和溶解，导致拟南芥小孢子死亡和雄性不育。

IF 2.9 4区生物学

Plant Reproduction Pub Date : 2024-09-01 Epub Date: 2024-02-13 DOI: 10.1007/s00497-024-00498-7

Sung-Aeong Oh, Soon Ki Park

{"title":"Ectopic expression of a thaumatin-like protein impairs the timely deposition and dissolution of callose during microsporogenesis, leading to microspore death and male sterility in Arabidopsis.","authors":"Sung-Aeong Oh, Soon Ki Park","doi":"10.1007/s00497-024-00498-7","DOIUrl":"10.1007/s00497-024-00498-7","url":null,"abstract":"Key message: Two pollen-preferential thaumatin-like proteins show both common and distinctive expression profiles. Precocious expression of one of them drastically disturbs timely deposition and dissolution of callose during microsporogenesis, leading to microspore death. Thaumatin-like proteins (TLPs), members of the pathogenesis-related protein family 5 (PR-5), are involved in plant defenses against biotic and abiotic stresses through antifungal activity and enhanced tolerance. Accordingly, studies on TLPs have focused on their responses to various pathogens and stresses and on engineering agronomically valuable crops that can be cultivated in suboptimal environments. On the other hand, the role of TLP members in plant development and their genetic regulation remains largely unexplored. Recently, we reported that the generative cell internalization after pollen mitosis I, an essential pollen patterning step for the nonmotile sperm cell delivery through a pollen tube, depends on STICKY GENERATIVE CELL which suppresses callose deposition in the nascent generative cell and interacts with a germline cell preferential GCTLP1 in Arabidopsis. Here, we additionally identified GCTLP2 which is similarly expressed in the germline cells. We generated various transgenic lines and examined their expressions and phenotypes to elucidate GCTLP functions during pollen development. Expression profiles suggest two GCTLP proteins may have common but also distinctive roles during pollen development. Importantly, ectopic expression analyses show that precocious expression of GCTLP2 severely disturbs the timely deposition and degradation of callose during microsporogenesis which is essential to produce viable microspores. Therefore, our study broadens the knowledge of TLP function and callose regulation for successful pollen development in Arabidopsis.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139730991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seedless fruit in Annona squamosa L. is monogenic and conferred by INO locus deletion in multiple accessions. Annona squamosa L.的无籽果实是单基因遗传的，由 INO 基因座缺失在多个品种中遗传。

IF 2.9 4区生物学

Plant Reproduction Pub Date : 2024-06-01 Epub Date: 2023-05-09 DOI: 10.1007/s00497-023-00464-9

Bruno Rafael Alves Rodrigues, Charles S Gasser, Samy Pimenta, Marlon Cristian Toledo Pereira, Silvia Nietsche

{"title":"Seedless fruit in Annona squamosa L. is monogenic and conferred by INO locus deletion in multiple accessions.","authors":"Bruno Rafael Alves Rodrigues, Charles S Gasser, Samy Pimenta, Marlon Cristian Toledo Pereira, Silvia Nietsche","doi":"10.1007/s00497-023-00464-9","DOIUrl":"10.1007/s00497-023-00464-9","url":null,"abstract":"Key message: Inheritance of the presence/absence of seeds in Annona squamosa is mediated by a single fully recessive gene and is caused by a deletion of the INNER NO OUTER (INO) locus. For some fruits, seedless varieties are desirable for consumption and processing. In the sugar apple tree (Annona squamosa L.), the seedless trait in the Thai seedless (Ts) and Brazilian seedless (Bs) accessions was associated with defective ovules and an apparent deletion of the INNER NO OUTER (INO) ovule development gene locus. Segregation analysis of F2 and backcross descendants of crosses of Bs to fertile wild-type varieties in this species with a multi-year generation time showed that seedlessness was recessive and controlled by a single locus. Comparison of whole genome sequence of a wild-type plant and a third accession, Hawaiian seedless (Hs), identified a 16 kilobase deletion including INO in this line. Ts and Bs lines were shown to have an identical deletion, indicating a common origin from a single deletion event. Analysis of microsatellite markers could not preclude the possibility that all three seedless accessions are vegetatively propagated clones. The sequence of the deletion site enabled a codominant assay for the wild-type and mutant genes allowing observation of complete cosegregation of the seedless/defective ovule phenotype with the INO deletion, showing maximal separation of less than 3.5 cM. The observed deletion is the only significant difference between the wild-type and Hs line over 587 kilobases, likely encompassing much more than 3.5 cM, showing that the deletion is the cause of seedless trait. The codominant markers and obtained progenies will be useful for introgression of the seedless trait into elite sugar apple lines and into other Annonas through interspecific crossings.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11180160/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9432324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Looking for non-hermaphrodite cacti: multidisciplinary studies in Gymnocalycium bruchii endemic to central Argentina. 寻找无雌雄同体的仙人掌：对阿根廷中部特有的Gymnocalycium bruchii进行多学科研究。

IF 3.4 4区生物学

Plant Reproduction Pub Date : 2024-06-01 Epub Date: 2023-03-20 DOI: 10.1007/s00497-023-00461-y

Natalia E Delbón, Nayla L Aliscioni, Marina Lorenzati, Sergio García, Rodrigo B Singer, Diego E Gurvich

{"title":"Looking for non-hermaphrodite cacti: multidisciplinary studies in Gymnocalycium bruchii endemic to central Argentina.","authors":"Natalia E Delbón, Nayla L Aliscioni, Marina Lorenzati, Sergio García, Rodrigo B Singer, Diego E Gurvich","doi":"10.1007/s00497-023-00461-y","DOIUrl":"10.1007/s00497-023-00461-y","url":null,"abstract":"Key message: Through a multidisciplinary study we found that Gymnocalycium bruchii, an endemic cactus from central Argentina, acts as a dioecious species, which is the first record in this genus. Cactaceae species are typically hermaphroditic; however, about 2% have other different reproductive systems. These non-hermaphroditic species may develop sexual dimorphism in flowers or other reproductive, vegetative or ecological traits, besides a specific breeding system and floral ontogeny. Therefore, multidisciplinary research is necessary to fully understand reproduction in those species. For this purpose, we studied Gymnocalicium bruchii, a globose cactus endemic to central Argentina that is presumably dioecious or gynodioecious. We made observations in two natural and two cultivated populations. We made morphological observations of plants and flowers, and performed quantitative analyses to determine the sex ratio, size of plants and flowers, flower production, fruiting, among other variables. We performed hand-pollination, self-fertilization and free-pollination tests to determine the breeding system. Finally, we studied the anatomy and ontogeny of the reproductive organs using permanent histological slides of flower morphs at different stages. Our results confirm that Gymnocalicium bruchii is a dioecious species. Female flowers have atrophied anthers and a functional gynoecium that produces fruits and seeds. Male flowers are bigger and have a functional androecium but a sterile gynoecium. In the cultivated population, the sex ratio was 1/1, whereas the number of male individuals was higher in both natural populations. Pollination tests corroborated dioecy. Ontogenetic studies revealed that in female flowers the anthers collapse before microspore maturation, while in male flowers the gynoecium shows normal development of the ovary, style, stigma, and ovules; however, the latter are never fertilized.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9513143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Factors specifying sex determination in maize. 玉米中决定性别的因素。

IF 2.9 4区生物学

Plant Reproduction Pub Date : 2024-06-01 Epub Date: 2023-11-15 DOI: 10.1007/s00497-023-00485-4

Cristina Guerrero-Méndez, María Jazmín Abraham-Juárez

引用次数: 0

EXPANSIN15 is involved in flower and fruit development in Arabidopsis. EXPANSIN15 参与拟南芥花和果实的发育。

IF 2.9 4区生物学

Plant Reproduction Pub Date : 2024-06-01 Epub Date: 2024-01-29 DOI: 10.1007/s00497-023-00493-4

Judith Jazmin Bernal-Gallardo, Karla L González-Aguilera, Stefan de Folter

{"title":"EXPANSIN15 is involved in flower and fruit development in Arabidopsis.","authors":"Judith Jazmin Bernal-Gallardo, Karla L González-Aguilera, Stefan de Folter","doi":"10.1007/s00497-023-00493-4","DOIUrl":"10.1007/s00497-023-00493-4","url":null,"abstract":"Key message: EXPANSIN15 is involved in petal cell morphology and size, the fusion of the medial tissues in the gynoecium and expansion of fruit valve cells. It genetically interacts with SPATULA and FRUITFULL. Cell expansion is fundamental for the formation of plant tissues and organs, contributing to their final shape and size during development. To better understand this process in flower and fruit development, we have studied the EXPANSIN15 (EXPA15) gene, which showed expression in petals and in the gynoecium. By analyzing expa15 mutant alleles, we found that EXPA15 is involved in petal shape and size determination, by affecting cell morphology and number. EXPA15 also has a function in fruit size, by affecting cell size and number. Furthermore, EXPA15 promotes fusion of the medial tissues in the gynoecium. In addition, we observed genetic interactions with the transcription factors SPATULA (SPT) and FRUITFULL (FUL) in gynoecium medial tissue fusion, style and stigma development and fruit development in Arabidopsis. These findings contribute to the importance of EXPANSINS in floral and fruit development in Arabidopsis.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11180156/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139571757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0