A semi in vivo pollination technique to assess the level of gametophytic self-incompatibility and pollen tube growth in pear (Pyrus communis L.).

IF 2.9 4区 生物学 Q2 PLANT SCIENCES
Plant Reproduction Pub Date : 2022-06-01 Epub Date: 2022-01-15 DOI:10.1007/s00497-021-00435-y
Hanne Claessen, Bram Van de Poel, Wannes Keulemans, Nico De Storme
{"title":"A semi in vivo pollination technique to assess the level of gametophytic self-incompatibility and pollen tube growth in pear (Pyrus communis L.).","authors":"Hanne Claessen,&nbsp;Bram Van de Poel,&nbsp;Wannes Keulemans,&nbsp;Nico De Storme","doi":"10.1007/s00497-021-00435-y","DOIUrl":null,"url":null,"abstract":"<p><strong>Key message: </strong>We describe a semi in vivo pollination technique to determine the compatibility relation between different pear cultivars. This assay provides a valuable addition to existing tools in GSI research. The gametophytic self-incompatibility (GSI) system in Pyrus inhibits fertilization by pollen that shares one of the two S-alleles of the style. Depending on their S-locus genotype, two pear cultivars therefore either show a cross-compatible, semi-compatible or incompatible interaction. Because GSI greatly influences seed and fruit set, accurate knowledge of the compatibility type of a cultivar is key for both pear fruit production and breeding. Currently, compatibility relations between different pear cultivars are generally assessed via S-genotyping. However, this approach is restricted to the currently known S-alleles in pear, and does not provide functional assessment of the level of (self-)incompatibility. We here present an optimized semi in vivo pollination assay, that enables quantitative analysis of (self-)incompatibility in pear, and that can also serve useful for more fundamental studies on pollen tube development and pollen-style interactions. This assay involves in vitro incubation of cut pollinated styles followed by microscopic counting of emerging pollen tubes at a specific time interval. The validity and selectivity of this method to determine compatibility interactions in pear is demonstrated in the cultivars \"Celina\" and \"Packham's Triumph.\" Overall, this technique constitutes a valuable tool for quantitatively determining in vivo pollen tube growth and (cross-)compatibility in pear.</p>","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"35 2","pages":"127-140"},"PeriodicalIF":2.9000,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Reproduction","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00497-021-00435-y","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/1/15 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 2

Abstract

Key message: We describe a semi in vivo pollination technique to determine the compatibility relation between different pear cultivars. This assay provides a valuable addition to existing tools in GSI research. The gametophytic self-incompatibility (GSI) system in Pyrus inhibits fertilization by pollen that shares one of the two S-alleles of the style. Depending on their S-locus genotype, two pear cultivars therefore either show a cross-compatible, semi-compatible or incompatible interaction. Because GSI greatly influences seed and fruit set, accurate knowledge of the compatibility type of a cultivar is key for both pear fruit production and breeding. Currently, compatibility relations between different pear cultivars are generally assessed via S-genotyping. However, this approach is restricted to the currently known S-alleles in pear, and does not provide functional assessment of the level of (self-)incompatibility. We here present an optimized semi in vivo pollination assay, that enables quantitative analysis of (self-)incompatibility in pear, and that can also serve useful for more fundamental studies on pollen tube development and pollen-style interactions. This assay involves in vitro incubation of cut pollinated styles followed by microscopic counting of emerging pollen tubes at a specific time interval. The validity and selectivity of this method to determine compatibility interactions in pear is demonstrated in the cultivars "Celina" and "Packham's Triumph." Overall, this technique constitutes a valuable tool for quantitatively determining in vivo pollen tube growth and (cross-)compatibility in pear.

梨配子体自交不亲和和花粉管生长的半体内授粉技术研究。
我们描述了一种半活体授粉技术来确定不同梨品种之间的亲和关系。该分析为GSI研究中的现有工具提供了有价值的补充。梨属植物配子体自交不亲和(GSI)系统抑制花柱两个s等位基因中共享一个的花粉受精。因此,根据它们的s位点基因型,两个梨品种表现出杂交亲和、半亲和或不亲和的相互作用。由于GSI对种子和坐果有很大的影响,因此准确了解一个品种的亲和性类型是梨果实生产和育种的关键。目前,梨品种间的亲和关系一般通过s基因分型进行鉴定。然而,这种方法仅限于目前已知的梨s等位基因,并且不能提供(自)不亲和水平的功能评估。本文提出了一种优化的半体内授粉实验方法,可以定量分析梨的自交不亲和性,也可以为花粉管发育和花粉柱相互作用的更基础研究提供帮助。该试验包括在体外培养的切花授粉花柱,然后在特定时间间隔内对新出现的花粉管进行显微计数。以“塞丽娜”和“派克汉姆的凯旋”品种为研究对象,验证了该方法测定梨亲和互作的有效性和选择性。总的来说,该技术是定量测定梨花粉管生长和(交叉)相容性的有价值的工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Plant Reproduction
Plant Reproduction PLANT SCIENCES-REPRODUCTIVE BIOLOGY
CiteScore
6.30
自引率
2.90%
发文量
19
期刊介绍: Plant Reproduction (formerly known as Sexual Plant Reproduction) is a journal devoted to publishing high-quality research in the field of reproductive processes in plants. Article formats include original research papers, expert reviews, methods reports and opinion papers. Articles are selected based on significance for the field of plant reproduction, spanning from the induction of flowering to fruit development. Topics incl … show all
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信