Plastid ribosome protein L5 is essential for post-globular embryo development in Arabidopsis thaliana.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Gilles Dupouy, Emma McDermott, Ronan Cashell, Anna Scian, Marcus McHale, Peter Ryder, Joelle de Groot, Noel Lucca, Galina Brychkova, Peter C McKeown, Charles Spillane
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引用次数: 5

Abstract

Plastid ribosomal proteins (PRPs) can play essential roles in plastid ribosome functioning that affect plant function and development. However, the roles of many PRPs remain unknown, including elucidation of which PRPs are essential or display redundancy. Here, we report that the nuclear-encoded PLASTID RIBOSOMAL PROTEIN L5 (PRPL5) is essential for early embryo development in A. thaliana, as homozygous loss-of-function mutations in the PRPL5 gene impairs chloroplast development and leads to embryo failure to develop past the globular stage. We confirmed the prpl5 embryo-lethal phenotype by generating a mutant CRISPR/Cas9 line and by genetic complementation. As PRPL5 underwent transfer to the nuclear genome early in the evolution of Embryophyta, PRPL5 can be expected to have acquired a chloroplast transit peptide. We identify and validate the presence of an N-terminal chloroplast transit peptide, but unexpectedly also confirm the presence of a conserved and functional Nuclear Localization Signal on the protein C-terminal end. This study highlights the fundamental role of the plastid translation machinery during the early stages of embryo development in plants and raises the possibility of additional roles of plastid ribosomal proteins in the nucleus.

Abstract Image

Abstract Image

Abstract Image

拟南芥质体核糖体蛋白L5对球状后胚胎发育至关重要。
质体核糖体蛋白(PRPs)在质体核糖体功能中发挥重要作用,影响植物的功能和发育。然而,许多PRPs的作用仍然未知,包括阐明哪些PRPs是必需的或显示冗余。在这里,我们报道了核编码的PLASTID核糖体蛋白L5 (PRPL5)对拟南拟南植物的早期胚胎发育至关重要,因为PRPL5基因的纯合子功能缺失突变会损害叶绿体发育并导致胚胎无法发育过球形阶段。我们通过产生突变的CRISPR/Cas9系和基因互补确认了prpl5胚胎致死性表型。由于PRPL5在胚胎进化的早期就转移到了核基因组中,PRPL5可能已经获得了叶绿体转运肽。我们鉴定并验证了一个n端叶绿体转运肽的存在,但出乎意料的是,也证实了在蛋白质c端存在一个保守的功能性核定位信号。这项研究强调了质体翻译机制在植物胚胎发育早期阶段的基本作用,并提出了质体核糖体蛋白在细胞核中发挥其他作用的可能性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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