Veterinary Quarterly最新文献_第5页

Metabolomic profiling of neat uterine luminal fluid in cows: local enrichment and nutritional modulation. 奶牛纯子宫腔液的代谢组学分析：局部富集和营养调节。

IF 3.9 2区农林科学

Veterinary Quarterly Pub Date : 2026-12-01 Epub Date: 2026-01-07 DOI: 10.1080/01652176.2025.2611849

Masroor Sagheer, Quinn A Hoorn, Mariangela Bc Maldonado, Kasey M Schalich, Nadia Ashrafi, Romana A Mimi, Stewart F Graham, Vimal Selvaraj, Peter J Hansen

{"title":"Metabolomic profiling of neat uterine luminal fluid in cows: local enrichment and nutritional modulation.","authors":"Masroor Sagheer, Quinn A Hoorn, Mariangela Bc Maldonado, Kasey M Schalich, Nadia Ashrafi, Romana A Mimi, Stewart F Graham, Vimal Selvaraj, Peter J Hansen","doi":"10.1080/01652176.2025.2611849","DOIUrl":"10.1080/01652176.2025.2611849","url":null,"abstract":"Uterine luminal fluid influences embryonic development and the subsequent phenotype of offspring, yet its detailed metabolomic composition remains poorly characterized. Here, minimally invasive transcervical techniques were employed to collect neat uterine fluid from postpartum dairy cows and cyclic beef cows to allow for metabolomic profiling via targeted mass spectrometry. Objectives were to 1) compare the metabolomic profile of uterine fluid with plasma in dairy cows and 2) assess the impact of dietary rumen-protected methionine and stage of estrous cycle (day 0 vs 7) on plasma and uterine fluid metabolomic profile in beef cows. Results revealed that the concentrations of many metabolites, including amino acids, signaling molecules (e.g. dopamine, gamma-aminobutyric acid) and lipids (e.g. ceramides, diacylglycerols), were higher in uterine fluid than in plasma. An oral bolus of rumen-protected methionine increased uterine concentration of methionine on day 0 of the estrous cycle. The uterine metabolome remained relatively stable between days 0 and 7 although there was temporal variability for a select number of metabolites (cysteine, methionine, methionine sulfoxide, asymmetric dimethylarginine, ceramides, and glycerophospholipids). Correlations between plasma and uterine fluid concentrations were strong or moderate for many amino acids. Collectively, these findings highlight that the uterine lumen is a specialized, selectively regulated biochemical compartment.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"46 1","pages":"2611849"},"PeriodicalIF":3.9,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12781943/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145913519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacteriophage vB_SalS_KY05 controls Salmonella in poultry without disrupting cecal microbiota composition. 噬菌体vB_SalS_KY05控制家禽沙门氏菌而不破坏盲肠菌群组成。

IF 3.9 2区农林科学

Veterinary Quarterly Pub Date : 2026-12-01 Epub Date: 2026-01-21 DOI: 10.1080/01652176.2026.2617464

Wun-Yuan Wu, Reuben Wang, Yu-Ning An, Yuan-Yu Lin

{"title":"Bacteriophage vB_SalS_KY05 controls Salmonella in poultry without disrupting cecal microbiota composition.","authors":"Wun-Yuan Wu, Reuben Wang, Yu-Ning An, Yuan-Yu Lin","doi":"10.1080/01652176.2026.2617464","DOIUrl":"10.1080/01652176.2026.2617464","url":null,"abstract":"Salmonellosis is a major food safety challenge in poultry production, requiring effective antibiotic alternatives. We isolated a polyvalent Tequintavirus, vB_SalS_KY05, from a Taiwanese poultry farm and evaluated its biological properties and in vivo efficacy against Salmonella enterica serovars Typhimurium and Enteritidis, and Escherichia coli. The objective of this study was to comprehensively characterize vB_SalS_KY05 and assess its potential as a biocontrol agent for poultry farming. Genome analysis confirmed a lytic lifestyle with no detectable virulence or antimicrobial resistance genes. The phage remained stable at pH 4-10, at 41 °C, and in water for 7 days. For improved biosafety, large-scale amplification was achieved by switching the propagation host to non-pathogenic E. coli K12. In vivo, SPF chickens challenged with S. Typhimurium received low-dose (105 PFU/mL) or high-dose (108 PFU/mL) phage via drinking water. Low-dose treatment reduced splenic Salmonella, improved albumin-to-globulin ratios, and enriched beneficial taxa like Lactobacillus crispatus and Blautia coccoides. In contrast, high-dose treatment resulted in phage-bacteria coexistence and increased potentially harmful taxa including Erysipelatoclostridium. Overall, cecal microbiota composition remained largely unchanged. These findings indicate vB_SalS_KY05 is a promising biocontrol candidate, highlighting the importance of dose optimization to enhance pathogen control while preserving gut microbiota stability in poultry production.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"46 1","pages":"2617464"},"PeriodicalIF":3.9,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12829423/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146020478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of the administration route and dose on the expression and antibody responses of a reporter and avian influenza self-amplifying mRNA vaccine in poultry. 给药途径和剂量对禽报告型和禽流感自扩增mRNA疫苗表达和抗体应答的影响

IF 3.9 2区农林科学

Veterinary Quarterly Pub Date : 2026-12-01 Epub Date: 2026-01-06 DOI: 10.1080/01652176.2025.2603307

Janne Snoeck, Xiaole Cui, Pieter Vervaeke, Niek N Sanders, An Garmyn

{"title":"Influence of the administration route and dose on the expression and antibody responses of a reporter and avian influenza self-amplifying mRNA vaccine in poultry.","authors":"Janne Snoeck, Xiaole Cui, Pieter Vervaeke, Niek N Sanders, An Garmyn","doi":"10.1080/01652176.2025.2603307","DOIUrl":"10.1080/01652176.2025.2603307","url":null,"abstract":"Vaccination is routinely used in industrial poultry to control infectious diseases. Vaccines based on mRNA and self-amplifying RNA (saRNA) are approved for human use, but research on their application in poultry is limited. In this study the saRNA vaccine platform is evaluated in poultry. First, a luciferase-encoding saRNA (luc-saRNA) was tested as a model vaccine across different administration routes and doses in broilers. High luciferase expression, and anti-luciferase antibodies were observed after intramuscular (IM), subcutaneous (SC), and in ovo (IO) administration. After a second Luc-saRNA injection, seroconversion rates and antibody titers increased in the IM and SC group to almost 100%. Higher doses of Luc-saRNA increased luciferase production. However, they did not linearly increase antibody production, as all tested doses (0.20-5.0 µg) elicited an equipotent immune response. A vaccination experiment with saRNA encoding the hemagglutinin head-domain (HA-HD) of H5N1 avian influenza showed hemagglutinin inhibition (HI) titers that are indicative for protection after a single injection and these titers remained above the protective threshold during 6 weeks without boosting. When boosted, the HI titers increased four-fold. This study confirms effective protein translation and immune response induction in chickens with IM or SC administered saRNA-LNPs, even at the lowest dose of 0.20 µg.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"46 1","pages":"2603307"},"PeriodicalIF":3.9,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12781945/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145913575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of five viruses commonly implicated with bovine respiratory disease using loop-mediated isothermal amplification. 利用环介导的等温扩增技术检测与牛呼吸道疾病有关的五种病毒。

IF 3.9 2区农林科学

Veterinary Quarterly Pub Date : 2026-12-01 Epub Date: 2026-01-12 DOI: 10.1080/01652176.2025.2607418

Josiah Levi Davidson, Murali Kannan Maruthumuthu, Mohamed Kamel, Suraj Mohan, Ana Pascual-Garrigos, Andres Dextre, Ruth Eunice Centeno-Delphia, Jacquelyn P Boerman, Deepti Pillai, Jennifer Koziol, Aaron Ault, Jon P Schoonmaker, Timothy A Johnson, Mohit S Verma

{"title":"Detection of five viruses commonly implicated with bovine respiratory disease using loop-mediated isothermal amplification.","authors":"Josiah Levi Davidson, Murali Kannan Maruthumuthu, Mohamed Kamel, Suraj Mohan, Ana Pascual-Garrigos, Andres Dextre, Ruth Eunice Centeno-Delphia, Jacquelyn P Boerman, Deepti Pillai, Jennifer Koziol, Aaron Ault, Jon P Schoonmaker, Timothy A Johnson, Mohit S Verma","doi":"10.1080/01652176.2025.2607418","DOIUrl":"10.1080/01652176.2025.2607418","url":null,"abstract":"Herein, we present novel quantitative loop-mediated isothermal amplification (qLAMP) and reverse-transcription qLAMP (RT-qLAMP) assays for the detection of five viruses implicated with the onset and progression of bovine respiratory disease (BRD): Bovine Alphaherpesvirus Type 1 (BHV-1), Bovine Adenovirus Type 3 (BAV-3), Bovine Respiratory Syncytial Virus (BRSV), Bovine Viral Diarrhea Virus Type 1 (BVDV-1), and Bovine Parainfluenza Virus Type 3 (BPIV-3). Using contrived samples spiked with whole viruses, our extraction-free assays have limits of detection between 30 and 1,057 copies per reaction (1.8% final sample concentration) with minimal sample processing. Using dual-tipped swabs and 1.4 mL resuspension volumes, limits of detection are on the order of 2 × 105 copies per swab for BAV-3 and BHV-1 and between 6.31 × 106 to 8.22 × 106 copies per swab for BPIV-3, BRSV, and BVDV-1. Analytical sensitivities ranged from 73 - 100% and analytical specificities ranged from 90 - 100%. Additionally, we introduced a streamlined pipeline to minimize the experimental workload to design, screen, select, and characterize LAMP performance for developing assays. Our assays support the development of colorimetric LAMP assays that enable the sensitive and specific detection of these viruses' chute side to aid in diagnosing and treating BRD. The associated pipeline enables more rapid development of LAMP-based diagnostic tools targeting emerging pathogens.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"46 1","pages":"2607418"},"PeriodicalIF":3.9,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12798664/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145953580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An underlying mechanism of bovine mastitis: PGE₂ regulates Staphylococcus aureus-induced inflammatory response through TLR2, TLR4, and NLRP3 in macrophages. 牛乳腺炎的潜在机制：PGE2通过巨噬细胞中的TLR2、TLR4和NLRP3调节金黄色葡萄球菌诱导的炎症反应。

IF 3.9 2区农林科学

Veterinary Quarterly Pub Date : 2026-12-01 Epub Date: 2026-01-17 DOI: 10.1080/01652176.2026.2615759

Zhiguo Gong, Zhuoya Yu, Peipei Ren, Shuangyi Zhang, Ruifeng Gao, Jiamin Zhao, Yixin Wang, Shaojie Qin, Wenhui Bao, Feng Shuang

{"title":"An underlying mechanism of bovine mastitis: PGE2 regulates Staphylococcus aureus-induced inflammatory response through TLR2, TLR4, and NLRP3 in macrophages.","authors":"Zhiguo Gong, Zhuoya Yu, Peipei Ren, Shuangyi Zhang, Ruifeng Gao, Jiamin Zhao, Yixin Wang, Shaojie Qin, Wenhui Bao, Feng Shuang","doi":"10.1080/01652176.2026.2615759","DOIUrl":"10.1080/01652176.2026.2615759","url":null,"abstract":"Staphylococcus aureus (S. aureus) evades host immunity by modulating macrophage functions, including immune regulation and phagocytosis, ultimately contributing to bovine mastitis. This study aimed to elucidate the molecular mechanisms of S. aureus-induced bovine mastitis from both host and pathogen perspectives, focusing on prostaglandin E2 (PGE2) as a key regulator. During bovine mastitis, macrophages were recruited into the mammary gland with elevated inflammatory mediators. S. aureus lipoproteins amplified inflammation by activating MAPK and NF-κB pathways via TLR2, TLR4, and NLRP3, leading to elevated secretion of mediators, including PGE2, in bBMMs. Inhibition of TLR2, TLR4, or NLRP3 decreased COX-2 and mPGES-1 expression, suppressing PGE2 synthesis, while inhibition of COX-2 or mPGES-1 can regulate the expression of TLR2 and NLRP3, as well as the activation of MAPKs and NF-κB signaling pathways. Excess PGE2 can regulate inflammation and phagocytosis mediated by TLR2, TLR4, and NLRP3. S. aureus lipoproteins promote PGE2 synthesis via TLR2, TLR4, and NLRP3 signaling, while PGE2, in turn, modulates receptor activity, inflammation, and phagocytosis. These findings reveal crucial functional cross-talk between PGE2 and innate immune receptors in S. aureus-induced mastitis, suggesting that targeting this interaction may provide novel therapeutic strategies.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"46 1","pages":"2615759"},"PeriodicalIF":3.9,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12818336/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145991815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Campylobacter bilis caused spotty liver syndrome in laying hens in the Netherlands. 在荷兰，胆汁弯曲杆菌引起蛋鸡斑肝综合征。

IF 3.9 2区农林科学

Veterinary Quarterly Pub Date : 2026-12-01 Epub Date: 2026-02-14 DOI: 10.1080/01652176.2026.2628724

Erik van Engelen, Miriam Koene, Robert Jan Molenaar, Annet Heuvelink

引用次数: 0

Ultrasonographic evaluation of the normal gastrointestinal wall in dogs and cats: a systematic review on study design and imaging outcomes. 狗和猫正常胃肠壁的超声评价：研究设计和成像结果的系统回顾。

IF 3.9 2区农林科学

Veterinary Quarterly Pub Date : 2026-12-01 Epub Date: 2026-02-09 DOI: 10.1080/01652176.2026.2622732

Marisa Esteves-Monteiro, Margarida Duarte-Araújo, Clara Landolt, Cláudia S Baptista

{"title":"Ultrasonographic evaluation of the normal gastrointestinal wall in dogs and cats: a systematic review on study design and imaging outcomes.","authors":"Marisa Esteves-Monteiro, Margarida Duarte-Araújo, Clara Landolt, Cláudia S Baptista","doi":"10.1080/01652176.2026.2622732","DOIUrl":"10.1080/01652176.2026.2622732","url":null,"abstract":"Diagnostic ultrasound (US) is a noninvasive, cost-effective imaging modality widely used for evaluating the gastrointestinal (GI) tract in companion animals. It provides information on wall thickness and layer differentiation, allowing assessment of normal anatomy and pathological changes. Despite its diagnostic relevance, ultrasonographic reference values for the GI tract in dogs and cats remain inconsistent across publications. This study reviewed ultrasonographic characteristics of the normal GI wall in dogs and cats and compiled a consensus-based reference table for overall wall thickness and individual layer proportions to enhance clinical interpretation. A literature search of PubMed and Scopus identified studies assessing the ultrasonographic features of normal GI segments, from stomach to colon, in healthy dogs and cats. Twelve studies met the inclusion criteria: six focused on dogs and six on cats. Reference values for GI wall thickness and its layers were reported in both species. However, discrepancies were noted in weight-based classifications for dogs, and the stomach of adult dogs remains poorly studied. Moreover, evaluation of gastric rugal and inter-rugal folds remains limited in this species. US is valuable for GI assessment, but dispersion of reference values across studies may hinder accessibility. Establishing standardized ultrasonographic parameters could improve diagnostic accuracy and clinical decision-making.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"46 1","pages":"2622732"},"PeriodicalIF":3.9,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12888347/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146144545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antiviral effect of poly(styrene 4-sulfonate) (PSSNa) on feline calicivirus oral infections in cats-field study. 聚苯乙烯磺酸钠（PSSNa）对猫口腔杯状病毒感染的抗病毒作用——实地研究。

IF 3.9 2区农林科学

Veterinary Quarterly Pub Date : 2026-12-01 Epub Date: 2026-01-19 DOI: 10.1080/01652176.2026.2616395

Aleksandra Synowiec, Magdalena Pachota, Martyna Krejmer-Rabalska, Daria Ziemann, Krzysztof Szczubiałka, Michal Jank, Lukasz Rabalski, Maria Nowakowska, Jerzy P Gawor, Krzysztof Pyrć

{"title":"Antiviral effect of poly(styrene 4-sulfonate) (PSSNa) on feline calicivirus oral infections in cats-field study.","authors":"Aleksandra Synowiec, Magdalena Pachota, Martyna Krejmer-Rabalska, Daria Ziemann, Krzysztof Szczubiałka, Michal Jank, Lukasz Rabalski, Maria Nowakowska, Jerzy P Gawor, Krzysztof Pyrć","doi":"10.1080/01652176.2026.2616395","DOIUrl":"10.1080/01652176.2026.2616395","url":null,"abstract":"Feline calicivirus (FCV) infection causes nasal discharge, oral mucosa inflammation, ulcerations, gingivitis, and conjunctivitis, often progressing to chronic gingivostomatitis, severe pneumonia, and fatal systemic infections. With no antivirals currently available, poly(sodium 4-styrene sulfonate) (PSSNa) was identified in 2019 as a safe inhibitor in vitro. In this preliminary single-center, randomized, double-blind, placebo-controlled field study, we further characterized the PSSNa's safety profile and tested its efficacy in cats after topical oral application. Twenty-eight cats were enrolled in the study, and they were initially treated with standard dental therapy, followed by adjuvant local oral application of PSSNa or placebo. After 4 weeks, PSSNa demonstrated a favorable safety profile with no adverse effects. The treatment group showed a significant decrease in viral load (p = 0.001) compared to placebo (p = 0.012). Disease symptoms improved significantly, though the oral health index remained unchanged. Additionally, PSSNa showed activity against multiple genetically diverse isolates, indicating a potential, exploratory link between genetic background and treatment outcome. Summarizing, this study presents initial data on the efficacy and tolerability of PSSNa treatment for FCV infections in cats. Nevertheless, several significant limitations should be acknowledged, including inconsistent drug administration by owners, non-sterile housing, sample size, variable oral disease severity, and concurrent treatments.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"46 1","pages":"2616395"},"PeriodicalIF":3.9,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12818315/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145999615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring thyroid function in main domestic ruminants: a scoping review of physio-anatomy, diseases and diagnostic tools. 探索国内主要反刍动物的甲状腺功能：生理解剖、疾病和诊断工具的范围综述。

IF 5.2 2区农林科学

Veterinary Quarterly Pub Date : 2026-12-01 Epub Date: 2025-12-24 DOI: 10.1080/01652176.2025.2603304

Justine Eppe, Patrick Petrossians, Frédéric Rollin, Sandrina Vandenput, Hugues Guyot

{"title":"Exploring thyroid function in main domestic ruminants: a scoping review of physio-anatomy, diseases and diagnostic tools.","authors":"Justine Eppe, Patrick Petrossians, Frédéric Rollin, Sandrina Vandenput, Hugues Guyot","doi":"10.1080/01652176.2025.2603304","DOIUrl":"10.1080/01652176.2025.2603304","url":null,"abstract":"While thyroid physiology has been studied in domestic ruminants, many uncertainties remain. In fact, this metabolism is rarely assessed in routine veterinary practice, and diseases of the thyroid gland or its metabolism are poorly documented in domestic ruminants. This scoping review aims to summarize current knowledge in anatomy, physiology, diseases, and diagnostic methods related to thyroid function in domestic ruminants. A structured research methodology was followed using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) extension for scoping reviews. Four databases were used: CAB Abstracts, Embase, PubMed and Scopus. Selection and screening process of the identified studies, as well as data extraction, were managed using Covidence software. Finally, 206 studies were included. Most studies involved cattle (n = 104), followed by sheep (n = 65) and goats (n = 28). The main study topic was on thyroid physiology (n = 127), followed by diseases (n = 48), diagnostic methods (n = 22) and histology (n = 9). Although many studies addressed the anatomy and physiology of the thyroid gland, few confirmed the euthyroid status (having a normally functioning thyroid gland) of these animals, warranting cautious interpretation of the results. Hypothyroidism is the most documented thyroid disease in ruminants, predominantly caused by iodine deficiency. The physiology of the thyroid gland has been extensively studied in relation to heat stress, reproduction, animal production and nutrition. However, there is much less literature available on diseases described in domestic ruminants and their diagnostic methods. Diagnostic tools for assessing thyroid metabolism in ruminants include assays for total thyroxine, total triiodothyronine, bovine thyroid stimulating hormone, total serum iodine, milk iodine, urine iodine, and plasmatic inorganic iodine.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"46 1","pages":"2603304"},"PeriodicalIF":5.2,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12777779/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145822178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Machine learning-assisted detection of canine mammary tumors using serum autoantibody signatures. 基于血清自身抗体特征的机器学习辅助犬乳腺肿瘤检测。

IF 3.9 2区农林科学

Veterinary Quarterly Pub Date : 2026-12-01 Epub Date: 2026-01-21 DOI: 10.1080/01652176.2026.2617470

Bluest Lan, Chia-Yu Chang, Shin-Wu Liu, Chih-Ching Wu, Kuan-Ming Lai, Hao-Ping Liu

{"title":"Machine learning-assisted detection of canine mammary tumors using serum autoantibody signatures.","authors":"Bluest Lan, Chia-Yu Chang, Shin-Wu Liu, Chih-Ching Wu, Kuan-Ming Lai, Hao-Ping Liu","doi":"10.1080/01652176.2026.2617470","DOIUrl":"10.1080/01652176.2026.2617470","url":null,"abstract":"Canine mammary tumors (CMTs) are the most common neoplasms in intact female dogs, yet early detection remains challenging due to the lack of clinically validated, noninvasive biomarkers. This study aimed to develop a noninvasive diagnostic model for CMT detection by integrating serum autoantibody biomarkers with machine learning. Serum samples from 154 dogs with mammary tumors (31 benign, 123 malignant) and 39 healthy controls were analyzed using a custom multiplex immunoassay detecting autoantibodies against AGR2, HAPLN1, IGFBP5, and TYMS, normalized to anti-BSA levels. Median fluorescence intensity (MFI), standardized autoantibody ratios, and their combination, together with clinical variables, were used to train random forest classifiers. The model based on standardized autoantibody ratios achieved the best performance, with an AUC of 0.79 (sensitivity 75.3%, specificity 74.4%) for overall CMT detection; 0.78 (92.7%, 61.5%) for malignant CMTs; and 0.77 (82.2%, 71.8%) for early-stagemalignancies. Assuming a CMT prevalence of 0.5 in the hospital-referred population, the positive and negative predictive values ranged from 0.74-0.75 and 0.75-0.91, respectively. This proof-of-concept study demonstrates that a machine learning-assisted multiplex autoantibody assay offers a feasible noninvasive approach for CMT detection. Further validation in larger, independent cohorts is warranted to support clinical translation in veterinary oncology.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"46 1","pages":"2617470"},"PeriodicalIF":3.9,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12825649/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146013333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0