Veterinary Quarterly最新文献_第5页

Pathological, immunological and molecular epidemiological analysis of lumpy skin disease virus in Indian cattle during a high-mortality epidemic. 高死亡率流行病期间印度牛身上块状皮肤病病毒的病理学、免疫学和分子流行病学分析。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-09-05 DOI: 10.1080/01652176.2024.2398211

Gundallhalli Bayyappa Manjunathareddy, Mani Saminathan, Lalasangi Sanjeevakumar, Sugana Rao, Murali Dinesh, Kuldeep Dhama, Karam Pal Singh, Bhupendra Nath Tripathi

{"title":"Pathological, immunological and molecular epidemiological analysis of lumpy skin disease virus in Indian cattle during a high-mortality epidemic.","authors":"Gundallhalli Bayyappa Manjunathareddy, Mani Saminathan, Lalasangi Sanjeevakumar, Sugana Rao, Murali Dinesh, Kuldeep Dhama, Karam Pal Singh, Bhupendra Nath Tripathi","doi":"10.1080/01652176.2024.2398211","DOIUrl":"10.1080/01652176.2024.2398211","url":null,"abstract":"Lumpy skin disease (LSD) is an economically significant, emerging viral disease of Cattle and Buffaloes. This study aimed to investigate the causes of high mortality in a recent LSD epidemic in India. We examined 1618 animals across seventy outbreaks and conducted post-mortem on 48 cattle out of 513 clinically suspected LSD cases. The morbidity, mortality and case fatality rates recorded were 31.70%, 2.97 and 9.37% respectively. Disease stages were categorized as early (20.81%), mid (42.02%), and late (37.17%) and the distribution of skin lesions was classified as mild (34.14%), moderate (39.39%), and severe (26.47%). Post-mortem findings revealed systemic infection with necrotic and ulcerative nodules on multiple internal organs. Histologically, necrotizing vasculitis and mononuclear cell infiltration with intracytoplasmic inclusions were observed in various organs. The highest viral load was found in skin nodules/scabs, trachea, tongue, and lymph nodes. The viral load was significantly higher in mid- and late-stages of skin nodules and internal organs; whereas, blood from early-stage showed high viral load. The expression of Th1-type and Th2-type cytokines varied significantly across different stages of the disease. The downregulation of the apoptotic intrinsic and upregulation of the extrinsic pathway genes, suggesting that the latter plays a role in LSDV infection. Genetic analysis revealed that the LSD virus (LSDV) isolates were derived from a Kenyan ancestral strain with unique nucleotide changes in RPO30 and P32 gene. In conclusion, the high mortality in the recent Indian LSD epidemic can be attributed to a newly identified, highly virulent strain of LSDV causing systemic infection.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-22"},"PeriodicalIF":7.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11378666/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142134405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of clopidogrel, hypercoagulability, and platelet count in dogs undergoing splenectomy for splenic masses. 对因脾脏肿块接受脾脏切除术的狗进行氯吡格雷、高凝状态和血小板计数评估。

IF 6.4 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-06-01 DOI: 10.1080/01652176.2024.2347926

Guk-Il Joung, Jeong-Yeol Bae, Jung-Il Kim, Jin-Young Kim, Joong-Hyun Song

{"title":"Evaluation of clopidogrel, hypercoagulability, and platelet count in dogs undergoing splenectomy for splenic masses.","authors":"Guk-Il Joung, Jeong-Yeol Bae, Jung-Il Kim, Jin-Young Kim, Joong-Hyun Song","doi":"10.1080/01652176.2024.2347926","DOIUrl":"10.1080/01652176.2024.2347926","url":null,"abstract":"Dogs that had splenectomy are predisposed to fatal thrombotic conditions, and thrombocytosis is a risk factor for post-splenectomy hypercoagulability. However, in veterinary medicine, there are no specific therapeutic approaches for managing this hypercoagulability. This study aimed to determine the preventive effect of clopidogrel on post-operative hypercoagulability during the first 2 weeks post-splenectomy in dogs with splenic masses. This study included 12 dogs that had splenectomy. Seven dogs received no treatment (group A), and five were treated with clopidogrel (group B). Clopidogrel was loaded at 10 mg/kg on day 2 and continued at 2 mg/kg until day 14. Blood samples were collected on the day of surgery and 2, 7, and 14 days after splenectomy in both groups. In group B, thromboelastography (TEG) was performed on the same days. In group A, there was significant elevation of platelet counts on days 7 (p = 0.007) and 14 (p = 0.001) compared to day 0. In group B, the platelet counts were significantly elevated on day 7 (p = 0.032) but no significant difference was found on day 14 compared to day 0. Platelet counts on day 14 were significantly higher in group A than in group B (p = 0.03). The lower platelet counts were correlated with alterations in TEG parameters, and no significant differences were found in the K and α-angle values at all postoperative assessment points compared to day 0. Our study suggests that clopidogrel may reduce post-operative thrombocytosis and hypercoagulability in dogs that undergo splenectomy for splenic masses.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-8"},"PeriodicalIF":6.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11146242/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141186643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyomavirus surveillance in cetaceans of Brazil: first detection of polyomavirus in Guiana dolphins (Sotalia guianensis). 巴西鲸目动物多瘤病毒监测：首次在圭亚那海豚（Sotalia guianensis）中发现多瘤病毒。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-10-20 DOI: 10.1080/01652176.2024.2413185

Aricia Duarte-Benvenuto, Josué Díaz-Delgado, Ana Carolina Ewbank, Kátia R Groch, Vanessa Dal Bianco, Samira Costa-Silva, Roberta Zamana-Ramblas, Cíntia Favero, Adriana Castaldo Colosio, Hernani da Cunha Gomes Ramos, Elitieri Santos-Neto, Jose Lailson-Brito, Vitor L Carvalho, Vanessa L Ribeiro, Carolina Pacheco Bertozzi, Caroline F Pessi, Irene Sacristán, José Luiz Catão-Dias, Carlos Sacristán

{"title":"Polyomavirus surveillance in cetaceans of Brazil: first detection of polyomavirus in Guiana dolphins (Sotalia guianensis).","authors":"Aricia Duarte-Benvenuto, Josué Díaz-Delgado, Ana Carolina Ewbank, Kátia R Groch, Vanessa Dal Bianco, Samira Costa-Silva, Roberta Zamana-Ramblas, Cíntia Favero, Adriana Castaldo Colosio, Hernani da Cunha Gomes Ramos, Elitieri Santos-Neto, Jose Lailson-Brito, Vitor L Carvalho, Vanessa L Ribeiro, Carolina Pacheco Bertozzi, Caroline F Pessi, Irene Sacristán, José Luiz Catão-Dias, Carlos Sacristán","doi":"10.1080/01652176.2024.2413185","DOIUrl":"10.1080/01652176.2024.2413185","url":null,"abstract":"Polyomaviruses (PyVs) are small double-stranded DNA viruses able to infect species across all vertebrate taxa. In cetaceans, PyVs have been reported only in short-beaked common dolphin (Delphinus delphis), common bottlenose dolphin (Tursiops truncatus) and killer whale (Orcinus orca). Herein, we surveyed PyV in 119 cetaceans (29 mysticetes and 90 odontocetes) stranded along the Brazilian coast, from 2002 to 2022, comprising 18 species. DNA extracted from the lungs was tested using a nested PCR targeting the major capsid protein gene of PyV. Polyomavirus was detected in lung samples of 1.7% (2/119) cetaceans: two juvenile female Guiana dolphins (Sotalia guianensis) stranded in Rio de Janeiro (Rio de Janeiro state) and Guriri (Espírito Santo state), in 2018. Both retrieved sequences were identical and presented 93.3% amino acid identity with Zetapolyomavirus delphini, suggesting a novel species. On histopathology, one of the PyV-positive individuals presented basophilic intranuclear inclusion bodies morphologically consistent with polyomavirus in the lungs. Other available tissues from both cases were PyV-PCR-negative; however, both individuals tested positive for Guiana dolphin morbillivirus. To our knowledge, this is the first report of PyV infection in cetaceans of the Southern Hemisphere and the first description of a co-infection with morbillivirus.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-7"},"PeriodicalIF":7.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11492386/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142480146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of grazing on grassland or wood-pasture, slaughtering age and ageing time on meat production and quality in Podolian young bulls. 草地或林地放牧、屠宰年龄和老化时间对波多利亚小公牛产肉量和肉质的影响。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-08-01 DOI: 10.1080/01652176.2024.2381544

Francesco Giannico, Despoina Karatosidi, Claudia Carbonara, Marco Ragni, Simona Tarricone, Anna Caputi Jambrenghi, Luigi Tedone, Maria Antonietta Colonna

{"title":"Effects of grazing on grassland or wood-pasture, slaughtering age and ageing time on meat production and quality in Podolian young bulls.","authors":"Francesco Giannico, Despoina Karatosidi, Claudia Carbonara, Marco Ragni, Simona Tarricone, Anna Caputi Jambrenghi, Luigi Tedone, Maria Antonietta Colonna","doi":"10.1080/01652176.2024.2381544","DOIUrl":"10.1080/01652176.2024.2381544","url":null,"abstract":"Podolian cattle is an autochthonous breed well adapted to the harsh semi-arid environments of the Southern Italy regions; the extensive rearing system used for these indigenous animals is based on grazing on spontaneous pastures, such as grasslands or wood pastures These grazing systems respect animal welfare and enrich animal products with characteristics closely related to the feeding system and the farming environment. The aim of the present study was to characterize the nutritional value of a forage crop and a wood-pasture and to evaluate the effects of grazing by Podolian young bulls on the performances and meat quality in relation to the age at slaughter (14 or 18 months) and to the ageing time of meat (3, 9 or 14 days). The metabolizable energy and the gas production were greater in April and June for both pasture systems. Young bulls raised on the grassland showed greater slaughter weights (p < 0.05) as compared to those fed on the woodland system, at both the slaughtering ages. The Warner Bratzler Shear (WBS) force values for raw and cooked meat were not influenced by the pasture system but they significantly (p < 0.01) decreased in relation to the ageing time in all the groups. Ageing markedly (p < 0.05) increased the malondialdehyde (MDA) concentration from 3 to 14 days of storage, regardless of the pasture system and the slaughtering age. The n-6/n-3 polyunsaturated fatty acid ratio of meat was markedly lower in grassland animals, regardless of the age of slaughter. In conclusion, 18 months old grassland beef showed better performances and yield of meat cuts. Ageing for 9 days positively affected meat WBS without increasing MDA concentration.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-12"},"PeriodicalIF":7.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11299441/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141876580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Successful phage-antibiotic therapy of P. aeruginosa implant-associated infection in a Siamese cat. 用噬菌体抗生素成功治疗了一只暹罗猫的铜绿假单胞菌植入相关感染。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-05-10 DOI: 10.1080/01652176.2024.2350661

Ron Braunstein, Goran Hubanic, Ortal Yerushalmy, Sivan Oren-Alkalay, Amit Rimon, Shunit Coppenhagen-Glazer, Ofir Niv, Hilik Marom, Alin Barsheshet, Ronen Hazan

{"title":"Successful phage-antibiotic therapy of P. aeruginosa implant-associated infection in a Siamese cat.","authors":"Ron Braunstein, Goran Hubanic, Ortal Yerushalmy, Sivan Oren-Alkalay, Amit Rimon, Shunit Coppenhagen-Glazer, Ofir Niv, Hilik Marom, Alin Barsheshet, Ronen Hazan","doi":"10.1080/01652176.2024.2350661","DOIUrl":"10.1080/01652176.2024.2350661","url":null,"abstract":"Antibiotic-resistant pathogens are a growing global issue, leading to untreatable infectious diseases in both humans and animals. Personalized bacteriophage (phage) therapy, the use of specific anti-bacterial viruses, is currently a leading approach to combat antibiotic-resistant infections. The implementation of phage therapy has primarily been focused on humans, almost neglecting the impact of such infections on the health and welfare of companion animals. Pets also have the potential to spread resistant infections to their owners or the veterinary staff through zoonotic transmission. Here, we showcase personalized phage-antibiotic treatment of a cat with a multidrug-resistant Pseudomonas aeruginosa implant-associated infection post-arthrodesis surgery. The treatment encompassed a tailored combination of an anti-P. aeruginosa phage and ceftazidime, precisely matched to the pathogen. The phage was topically applied to the surgical wound while the antibiotic was administered intramuscularly. After two treatment courses spanning 7 and 3 weeks, the surgical wound, which had previously remained open for five months, fully closed. To the best of our knowledge, this is the first case of personalized phage therapy application in felines, which provides further evidence of the effectiveness of this approach. The successful outcome paves the way for personalized phage-antibiotic treatments against persistent infections therapy in veterinary practice.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-9"},"PeriodicalIF":7.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11089911/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140900281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A transmandibular lateral transsphenoidal navigated surgical approach to access a pituitary macroadenoma in a warmblood mare. 采用经颌外侧经蝶导航手术方法切除一匹温血母马的垂体大腺瘤。

IF 6.4 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-02-23 DOI: 10.1080/01652176.2023.2300947

Mathieu de Preux, Christina Precht, Julien Guevar, Claudia Graubner, Sebastian Thenhaus-Schnabel, Larissa Buser, Anton Lukes, Christoph Koch

{"title":"A transmandibular lateral transsphenoidal navigated surgical approach to access a pituitary macroadenoma in a warmblood mare.","authors":"Mathieu de Preux, Christina Precht, Julien Guevar, Claudia Graubner, Sebastian Thenhaus-Schnabel, Larissa Buser, Anton Lukes, Christoph Koch","doi":"10.1080/01652176.2023.2300947","DOIUrl":"10.1080/01652176.2023.2300947","url":null,"abstract":"A 16-year-old warmblood mare was referred with a progressive history of behavioral changes and left-sided blindness. Following neuroanatomical localization to the forebrain, magnetic resonance imaging of the head revealed a well-delineated, 4.5 cm in diameter, round pituitary mass causing marked compression of the midbrain and optic chiasm. Euthanasia was recommended but declined by the owners. Veterinary specialists and a human neurosurgeon collaboratively prepared for surgical case management. A novel navigated transmandibular lateral transsphenoidal approach was developed to access the region of the sella turcica and practiced on cadaver specimens. The horse was anesthetized and placed in sternal recumbency with the head above the heart line. Using a cone beam computed tomography (CBCT)-coupled navigation system, a navigated pin traversing the vertical ramus of the mandible and the lateral pterygoid muscle was placed in a direct trajectory to the predetermined osteotomy site of the basisphenoid bone. A safe corridor to the osteotomy site was established using sequential tubular dilators bypassing the guttural pouch, internal and external carotid arteries. Despite the use of microsurgical techniques, visualization of critical structures was limited by the long and narrow working channel. Whilst partial resection of the mass was achieved, iatrogenic trauma to the normal brain parenchyma was identified by intraoperative imaging. With consent of the owner the mare was euthanized under the same general anesthesia. Post-mortem magnetic resonance imaging and gross anatomical examination confirmed partial removal of a pituitary adenoma, but also iatrogenic damage to the surrounding brain parenchyma, including the thalamus.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-10"},"PeriodicalIF":6.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10896130/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139934086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bovine anaplasmosis in Zimbabwe: spatio-temporal distribution and environmental drivers. 津巴布韦的牛无形体病：时空分布与环境驱动因素。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-01-27 DOI: 10.1080/01652176.2024.2306210

Natasher Madyavanhu, Munyaradzi Davis Shekede, Samuel Kusangaya, Davies Mubika Pfukenyi, Sylvester Chikerema, Isaiah Gwitira

{"title":"Bovine anaplasmosis in Zimbabwe: spatio-temporal distribution and environmental drivers.","authors":"Natasher Madyavanhu, Munyaradzi Davis Shekede, Samuel Kusangaya, Davies Mubika Pfukenyi, Sylvester Chikerema, Isaiah Gwitira","doi":"10.1080/01652176.2024.2306210","DOIUrl":"10.1080/01652176.2024.2306210","url":null,"abstract":"Understanding the spatial and temporal distribution of Bovine anaplasmosis is crucial for identifying areas of high prevalence for targeted disease control. This research was aimed at modelling and mapping the B. anaplasmosis potential distribution, and identify hotspots as well as significant variables explaining the occurrence of the disease. The Getis Ord Gi* statistic for Hotspot analysis was used as well as MaxEnt ecological niche modelling. The effects of time, land-use, and agro-ecological regions on B. anaplasmosis occurrence were tested using Analysis of Variance (ANOVA). Results showed that several districts in Zimbabwe are suitable for the occurence of the disease for example Binga, Seke, Buhera, Kwekwe, Gweru, Mhondoro, Chegutu, Sanyati, and in the North: Mbire, Muzarabani, Mt Darwin, Shamva, Bindura, Zvimba and Makonde. Morbidity and mortality hotspots were detected in Gokwe-south, Kwekwe, and Chirumhanzu districts. Binga, Gokwe-south, Gutu, Hurungwe, Mazoe, Nkayi, Shamva, and Kwekwe districts also experienced high disease incidences. Temperature seasonality, precipitation seasonality, mean diurnal range, and isothermality were the most important variables in explaining 93% of B. anaplasmosis distribution. Unlike land-use and agro-ecological regions, time (months) had a significant effect on B. anaplasmosis occurrence with July and September having significantly (p < 0.05) higher cases and deaths than the rest of the months. The results of this study provide insights into the management strategies and control of B. anaplasmosis in Zimbabwe. It is thus concluded that geo-spatial techniques, combined with ecological niche modelling can provide useful insights into disease prevalence and distribution and hence can contribute to effective management and control of B. anaplasmosis in Zimbabwe.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-16"},"PeriodicalIF":7.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10823892/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139567611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A high titer antibody response against P22 protein immunocomplex is not correlated with protection in naturally tuberculosis-infected goats. 针对 P22 蛋白免疫复合物的高滴度抗体反应与天然结核病感染山羊的保护无关。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-11-19 DOI: 10.1080/01652176.2024.2429851

Ortega J, Agulló-Ros I, Roy A, Moreno I, Gómez-Buendía A, Romero B, Ferreras-Colino E, de Juan L, Domínguez M, Domínguez L, Risalde M A, Bezos J

{"title":"A high titer antibody response against P22 protein immunocomplex is not correlated with protection in naturally tuberculosis-infected goats.","authors":"Ortega J, Agulló-Ros I, Roy A, Moreno I, Gómez-Buendía A, Romero B, Ferreras-Colino E, de Juan L, Domínguez M, Domínguez L, Risalde M A, Bezos J","doi":"10.1080/01652176.2024.2429851","DOIUrl":"10.1080/01652176.2024.2429851","url":null,"abstract":"Caprine livestock are significant reservoirs of the Mycobacterium tuberculosis complex (MTBC), contributing to tuberculosis (TB) transmission among animals and humans. The P22 protein immunocomplex (P22PI), derived from bovine tuberculin, shows immunostimulating capacity and is used for TB diagnosis. This study assessed the immunogenicity and protective efficacy of P22PI in two groups of goats: 24 naïve goats (12 immunised, 12 controls) from a TB-free herd, and 24 infected goats (12 immunised, 12 controls), referred to as pre-infected animals, from a M. bovis-infected herd. Both were exposed for 5 months to M. bovis-naturally infected goats. Reactors to single and comparative intradermal tuberculin (SIT and SCIT, respectively) tests and interferon-gamma release assay (IGRA) significantly increased (p < 0.05) in both groups 5 months' post-exposure, with no significant differences between immunised and control animals. However, immunised animals exhibited a significantly higher (p < 0.05) antibody response against P22PI. Most naïve animals (83.3%) and all pre-infected animals developed TB-compatible lesions, with extensive necrosis in the lungs and associated lymph nodes, compared to 50% and 83.3% of control animals, respectively. These findings suggest that while P22PI stimulates an intense antibody response under the conditions of the present study, it does not confer protection against TB and may exacerbate disease severity.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"16-30"},"PeriodicalIF":7.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11578410/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142669997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TGFβ in malignant canine mammary tumors: relation with angiogenesis, immunologic markers and prognostic role. 恶性犬乳腺肿瘤中的 TGFβ：与血管生成、免疫标记物和预后作用的关系。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-08-20 DOI: 10.1080/01652176.2024.2390941

Maria Isabel Carvalho, Ricardo Silva-Carvalho, Justina Prada, Carla Pinto, Hugo Gregório, Luis Lobo, Isabel Pires, Felisbina L Queiroga

{"title":"TGFβ in malignant canine mammary tumors: relation with angiogenesis, immunologic markers and prognostic role.","authors":"Maria Isabel Carvalho, Ricardo Silva-Carvalho, Justina Prada, Carla Pinto, Hugo Gregório, Luis Lobo, Isabel Pires, Felisbina L Queiroga","doi":"10.1080/01652176.2024.2390941","DOIUrl":"10.1080/01652176.2024.2390941","url":null,"abstract":"Transforming growth factor-β (TGFβ) and FoxP3 regulatory T cells (Treg) are involved in human breast carcinogenesis. This topic is not well documented in canine mammary tumors (CMT). In this work, the tumoral TGFβ expression was assessed by immunohistochemistry in 67 malignant CMT and its correlation to previously determined FoxP3, VEGF, and CD31 markers and other clinicopathologic parameters was evaluated. The high levels of TGFβ were statistically significantly associated with skin ulceration, tumor necrosis, high histological grade of malignancy (HGM), presence of neoplastic intravascular emboli and presence of lymph node metastases. The observed levels of TGFβ were positively correlated with intratumoral FoxP3 (strong correlation), VEGF (weak correlation) and CD31 (moderate correlation). Tumors that presented a concurrent high expression of TGFβ/FoxP3, TGFβ/VEGF, and TGFβ/CD31 markers were statistically significantly associated with parameters of tumor malignancy (high HGM, presence of vascular emboli and nodal metastasis). Additionally, shorter overall survival (OS) time was statistically significantly associated with tumors with an abundant TGFβ expression and with concurrent high expression of TGFβ/FoxP3, TGFβ/VEGF, and TGFβ/CD31. The presence of lymph node metastasis increased 11 times the risk of disease-related death, arising as an independent predictor of poor prognosis in the multivariable analysis. In conclusion, TGFβ and Treg cells seem involved in tumor progression emerging as potential therapeutic targets for future immunotherapy studies.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-12"},"PeriodicalIF":7.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11340227/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142009927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New preclinical biomarkers for prion diseases in the cerebrospinal fluid proteome revealed by mass spectrometry. 质谱法揭示脑脊液蛋白质组中朊病毒疾病的新临床前生物标志物。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-11-09 DOI: 10.1080/01652176.2024.2424837

Sonia Pérez-Lázaro, Tomás Barrio, Susana B Bravo, Eloisa Sevilla, Alicia Otero, María Del Pilar Chantada-Vázquez, Inmaculada Martín-Burriel, Jesús R Requena, Juan J Badiola, Rosa Bolea

{"title":"New preclinical biomarkers for prion diseases in the cerebrospinal fluid proteome revealed by mass spectrometry.","authors":"Sonia Pérez-Lázaro, Tomás Barrio, Susana B Bravo, Eloisa Sevilla, Alicia Otero, María Del Pilar Chantada-Vázquez, Inmaculada Martín-Burriel, Jesús R Requena, Juan J Badiola, Rosa Bolea","doi":"10.1080/01652176.2024.2424837","DOIUrl":"10.1080/01652176.2024.2424837","url":null,"abstract":"Current diagnostic methods for prion diseases only work in late stages of the disease when neurodegeneration is irreversible. Therefore, biomarkers that can detect the disease before the onset of clinical symptoms are necessary. High-throughput discovery proteomics is of great interest in the search for such molecules. Here we used mass spectrometry to analyse the cerebrospinal fluid proteome in an animal prion disease: preclinical and clinical sheep affected with natural scrapie, and healthy sheep. Interestingly, we found 46 proteins in the preclinical stage that were significantly altered (p < 0.01) compared to healthy sheep, mainly associated with biological processes such as stress and inflammatory responses. Five of them were selected for validation by enzyme-like immunosorbent assay: synaptotagmin binding, cytoplasmic RNA interacting protein (SYNCRIP), involved in nucleic acid metabolism; phospholipase D3 (PLD3) and cathepsin D (CTSD), both related to lysosomal apoptosis; complement component 4 (C4), an element of the classical immune response; and osteopontin (SPP1), a proinflammatory cytokine. These proteins significantly increased in the preclinical stage and maintained their levels in the clinical phase, except for CTSD, whose concentration returned to basal levels in the clinical group. Further research is ongoing to explore their potential as preclinical biomarkers of prion diseases.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-15"},"PeriodicalIF":7.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11552261/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142631924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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