Veterinary Quarterly最新文献_第4页

Characterization of early-stage lesions and investigation on the role of mucosal trauma in hemorrhagic bowel syndrome in cattle. 牛出血性肠道综合征早期病变的特征和粘膜创伤作用的研究。

IF 6.4 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-06-04 DOI: 10.1080/01652176.2024.2360422

Bert De Jonge, Bart Pardon, Jozefien Callens, Koen Chiers

{"title":"Characterization of early-stage lesions and investigation on the role of mucosal trauma in hemorrhagic bowel syndrome in cattle.","authors":"Bert De Jonge, Bart Pardon, Jozefien Callens, Koen Chiers","doi":"10.1080/01652176.2024.2360422","DOIUrl":"10.1080/01652176.2024.2360422","url":null,"abstract":"Hemorrhagic bowel syndrome (HBS) is characterized by a dissecting intramucosal hematoma at the small bowel, causing obstruction and severe hemorrhage in dairy cattle. Recent investigation revealed the presence of early-stage lesions in cows affected by HBS. These are presumed to be the initial stage of the hematoma, as both share unique dissection of the lamina muscularis mucosae (LMM) as histological hallmark. Early-stage lesions of HBS have not been characterized in greater detail, and neither has the hypothesis of mucosal abrasion as etiology been explored. Therefore, the first objective of the present study was to characterize the morphology of early-stage lesions, by gross examination, histochemistry, immunohistochemistry and transmission electron microscopy. The second objective was to determine the effect of mucosal abrasion to the small intestine in an ex vivo model. A total of 86 early-stage lesions from 10 cows with HBS were characterized. No underlying alterations at the LMM were evident which could explain their occurrence. However, degeneration at the ultrastructural level of the LMM smooth muscle cells was present in 3 of 4 lesions, it is however unclear whether this is primary or secondary. Bacteriological examination did not reveal any association with a specific bacterium. Experimental-induced and early-stage lesions were gross and histologically evaluated and scored in three cows with HBS and seven controls. Experimentally induced lesions in both affected cows and controls, were histologically very similar to the naturally occurring early-stage lesions. Altogether, the results are suggestive for mucosal trauma to play a role in the pathogenesis of HBS.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-11"},"PeriodicalIF":6.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11151803/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141237642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial susceptibility and resistome of Actinobacillus pleuropneumoniae in Taiwan: a next-generation sequencing analysis. 台湾胸膜肺炎放线杆菌的抗菌药敏感性和耐药性组：新一代测序分析。

IF 6.4 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-04-30 DOI: 10.1080/01652176.2024.2335947

Chiao-Hsu Ke, Pan-Yun Lai, Feng-Yang Hsu, Po-Ren Hsueh, Ming-Tang Chiou, Chao-Nan Lin

{"title":"Antimicrobial susceptibility and resistome of Actinobacillus pleuropneumoniae in Taiwan: a next-generation sequencing analysis.","authors":"Chiao-Hsu Ke, Pan-Yun Lai, Feng-Yang Hsu, Po-Ren Hsueh, Ming-Tang Chiou, Chao-Nan Lin","doi":"10.1080/01652176.2024.2335947","DOIUrl":"https://doi.org/10.1080/01652176.2024.2335947","url":null,"abstract":"Actinobacillus pleuropneumoniae infection causes a high mortality rate in porcine animals. Antimicrobial resistance poses global threats to public health. The current study aimed to determine the antimicrobial susceptibilities and probe the resistome of A. pleuropneumoniae in Taiwan. Herein, 133 isolates were retrospectively collected; upon initial screening, 38 samples were subjected to next-generation sequencing (NGS). Over the period 2017-2022, the lowest frequencies of resistant isolates were found for ceftiofur, cephalexin, cephalothin, and enrofloxacin, while the highest frequencies of resistant isolates were found for oxytetracycline, streptomycin, doxycycline, ampicillin, amoxicillin, kanamycin, and florfenicol. Furthermore, most isolates (71.4%) showed multiple drug resistance. NGS-based resistome analysis revealed aminoglycoside- and tetracycline-related genes at the highest prevalence, followed by genes related to beta-lactam, sulfamethoxazole, florphenicol, and macrolide. A plasmid replicon (repUS47) and insertion sequences (IS10R and ISVAp11) were identified in resistant isolates. Notably, the multiple resistance roles of the insertion sequence IS10R were widely proposed in human medicine; however, this is the first time IS10R has been reported in veterinary medicine. Concordance analysis revealed a high consistency of phenotypic and genotypic susceptibility to florphenicol, tilmicosin, doxycycline, and oxytetracycline. The current study reports the antimicrobial characterization of A. pleuropneumoniae for the first time in Taiwan using NGS.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-13"},"PeriodicalIF":6.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11064736/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140871640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The development of a lateral flow immunochromatographic test strip for measurement of specific IgA and IgG antibodies level against porcine epidemic diarrhea virus in pig milk. 开发侧流免疫层析试纸，用于测量猪奶中猪流行性腹泻病毒特异性 IgA 和 IgG 抗体水平。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-11-21 DOI: 10.1080/01652176.2024.2429472

Patumporn Jermsutjarit, Dhithya Venkateswaran, Nitaya Indrawattana, Jessada Na Plord, Angkana Tantituvanont, Dachrit Nilubol

{"title":"The development of a lateral flow immunochromatographic test strip for measurement of specific IgA and IgG antibodies level against porcine epidemic diarrhea virus in pig milk.","authors":"Patumporn Jermsutjarit, Dhithya Venkateswaran, Nitaya Indrawattana, Jessada Na Plord, Angkana Tantituvanont, Dachrit Nilubol","doi":"10.1080/01652176.2024.2429472","DOIUrl":"10.1080/01652176.2024.2429472","url":null,"abstract":"Porcine epidemic diarrhea virus (PEDV) causes severe enteric disease and high mortality in neonatal piglets, leading to significant economic losses in the swine industry. Considering that passive lactogenic immunity is crucial for preventing infection in piglets, necessitating a rapid and accurate tool to measure immunity levels. This study aims to develop a lateral flow immunochromatographic strip (LFICS) to assess IgA and IgG antibodies in colostrum and milk, using PEDV S protein. The performance of LFICS was compared to viral neutralization (VN) and enzyme-linked immunosorbent assay (ELISA) as reference methods, with a visual scoring system applied for field monitoring. Colostrum (n = 82) and milk (n = 106) samples were analyzed, showing strong correlation with reference methods and no cross-reactivity with other pig pathogens. The LFICS exhibited high relative sensitivity (Se) and specificity (Sp), with colostrum showing 98.73% Se and 66.67% Sp for IgA, and 96.15% Se and 75.00% Sp for IgG. Milk demonstrated 95.60% Se and 80.00% Sp for IgA, and 84.88% Se and 85.00% Sp for IgG. These findings indicate that the LFICS is a reliable, simple, and rapid method for measuring PEDV-specific IgA and IgG levels, offering valuable support for monitoring herd immunity and evaluating vaccination programs.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-15"},"PeriodicalIF":7.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11583322/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142683500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum protein electrophoresis in European mink (Mustela lutreola): reference intervals and comparison of agarose gel electrophoresis and capillary zone electrophoresis. 欧洲水貂（Mustela lutreola）血清蛋白电泳：参考区间以及琼脂糖凝胶电泳和毛细管区带电泳的比较。

IF 6.4 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-02-22 DOI: 10.1080/01652176.2024.2318195

Sergio Villanueva-Saz, María Del Carmen Aranda, María de Los Ángeles Jiménez, Paloma Jimena de Andrés, Maite Verde, María Climent, María Eugenia Lebrero Berna, Diana Marteles Aragüés, Antonio Fernández

{"title":"Serum protein electrophoresis in European mink (Mustela lutreola): reference intervals and comparison of agarose gel electrophoresis and capillary zone electrophoresis.","authors":"Sergio Villanueva-Saz, María Del Carmen Aranda, María de Los Ángeles Jiménez, Paloma Jimena de Andrés, Maite Verde, María Climent, María Eugenia Lebrero Berna, Diana Marteles Aragüés, Antonio Fernández","doi":"10.1080/01652176.2024.2318195","DOIUrl":"10.1080/01652176.2024.2318195","url":null,"abstract":"Background: Knowledge of reference intervals for blood analytes, including serum protein fractions, is of great importance for the identification of infectious and inflammatory diseases and is often lacking in wild animal species.Material and methods: Serum samples were obtained from European minks enrolled in the breeding program (n = 55). Agarose gel electrophoresis (AGE) and capillary zone electrophoresis (CZE) were used to separate and identify protein fractions. Albumin, α1, α2, β, and γ-globulins fractions were identified in all mink sera by both electrophoresis methods. Reference intervals (90% CI) were determined following the 2008 guidelines of the Clinical Laboratory Standard Institute. The methods were compared using Passing-Bablok regression, Bland-Altman analysis, and Lin's concordance correlation.Results: A significant bias was found between methods for α1, α2, and γ-globulin. Lin's concordance correlation was considered unacceptable for α1, α2, and β-globulins. Differences for gender between methods were found for albumin and α2-globuins, which were higher for males than females. γ-globulins were higher for adults than young minks using both methods; however, α1 and α2-globulins were lower.Conclusion: Both methods are adequate for identifying serum protein disorders, but the AGE and CZE methods are not equivalent. Therefore, reference intervals for each technique are required.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-11"},"PeriodicalIF":6.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10896155/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139934087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunotherapy in mastitis: state of knowledge, research gaps and way forward. 乳腺炎的免疫疗法：知识现状、研究差距和前进方向。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-07-07 DOI: 10.1080/01652176.2024.2363626

Afnan Saleem, Sahar Saleem Bhat, Faith A Omonijo, Nazir A Ganai, Eveline M Ibeagha-Awemu, Syed Mudasir Ahmad

{"title":"Immunotherapy in mastitis: state of knowledge, research gaps and way forward.","authors":"Afnan Saleem, Sahar Saleem Bhat, Faith A Omonijo, Nazir A Ganai, Eveline M Ibeagha-Awemu, Syed Mudasir Ahmad","doi":"10.1080/01652176.2024.2363626","DOIUrl":"10.1080/01652176.2024.2363626","url":null,"abstract":"Mastitis is an inflammatory condition that affects dairy cow's mammary glands. Traditional treatment approaches with antibiotics are increasingly leading to challenging scenarios such as antimicrobial resistance. In order to mitigate the unwanted side effects of antibiotics, alternative strategies such as those that harness the host immune system response, also known as immunotherapy, have been implemented. Immunotherapy approaches to treat bovine mastitis aims to enhance the cow's immune response against pathogens by promoting pathogen clearance, and facilitating tissue repair. Various studies have demonstrated the potential of immunotherapy for reducing the incidence, duration and severity of mastitis. Nevertheless, majority of reported therapies are lacking in specificity hampering their broad application to treat mastitis. Meanwhile, advancements in mastitis immunotherapy hold great promise for the dairy industry, with potential to provide effective and sustainable alternatives to traditional antibiotic-based approaches. This review synthesizes immunotherapy strategies, their current understanding and potential future perspectives. The future perspectives should focus on the development of precision immunotherapies tailored to address individual pathogens/group of pathogens, development of combination therapies to address antimicrobial resistance, and the integration of nano- and omics technologies. By addressing research gaps, the field of mastitis immunotherapy can make significant strides in the control, treatment and prevention of mastitis, ultimately benefiting both animal and human health/welfare, and environment health.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-23"},"PeriodicalIF":7.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11232650/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141555901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxidative stress in relation to serotonin under general anaesthesia in dogs undergoing ovariectomy. 接受卵巢切除术的狗在全身麻醉状态下的氧化应激与血清素的关系。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-07-19 DOI: 10.1080/01652176.2024.2379319

Giuseppe Bruschetta, Fabio Leonardi, Patrizia Licata, Nicola Maria Iannelli, Rocìo Fernàndez-Parra, Fabio Bruno, Laura Messina, Giovanna Lucrezia Costa

{"title":"Oxidative stress in relation to serotonin under general anaesthesia in dogs undergoing ovariectomy.","authors":"Giuseppe Bruschetta, Fabio Leonardi, Patrizia Licata, Nicola Maria Iannelli, Rocìo Fernàndez-Parra, Fabio Bruno, Laura Messina, Giovanna Lucrezia Costa","doi":"10.1080/01652176.2024.2379319","DOIUrl":"10.1080/01652176.2024.2379319","url":null,"abstract":"Abdominal surgery such as ovariectomy is a traumatic event that can cause oxidative stress. The aim of the present study was to evaluate the concentration of serotonin in relation to ovariectomy-induced oxidative stress in dogs undergoing general anesthesia. Thirty-two female dogs, under general anesthesia, received meloxicam before surgery (0.2 mgkg-1 SC) and after surgery (0.1 mgkg-1 OS every 24 h). The physiological, hematological, and biochemical parameters: glycemia, aspartate transaminase (AST), alanine aminotransferase (ALT), total protein, albumin and BUN were evaluated. Oxidative stress was determined by malondialdehyde (MDA) assay, catalase (CAT), superoxide dismutase (SOD), myeloperoxidase (MPO) and butyrylcholinesterase (BuChe) at baseline, 36 and 48 h after the last administration of meloxicam. Serotonin (5-HT) concentration was also evaluated at baseline, 36 and 48 h after the last administration of meloxicam. Responses to surgical stimulus were evaluated. Physiological and hematological parameters they fell within the normal ranges for anesthetized dogs. Glycemia increased, albumin levels decreased after surgery. No rescue analgesia was required. MDA and 5-HT concentrations significantly increased from the baseline at 36 and 48 h after surgery (p < .001). 5-HT levels could be used as an indicator for oxidative stress induced by surgery and it might be employed for objectively quantifying the well-being of the surgical patient.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-8"},"PeriodicalIF":7.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11262201/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141724984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seroprevalence and risk factors of bluetongue virus in domestic cattle, sheep, goats and camels in Africa: a systematic review and meta-analysis. 非洲家畜牛、绵羊、山羊和骆驼的蓝舌病病毒血清流行率和风险因素：系统回顾和荟萃分析。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-08-30 DOI: 10.1080/01652176.2024.2396118

Bachir Medrouh, Amine Abdelli, Salem Belkessa, Yacine Ouinten, Malika Brahimi, Ahcène Hakem, Tahar Kernif, Steven M Singer, Hocine Ziam, Anastasios D Tsaousis, Pikka Jokelainen, Giovanni Savini, Edoardo Pasolli

{"title":"Seroprevalence and risk factors of bluetongue virus in domestic cattle, sheep, goats and camels in Africa: a systematic review and meta-analysis.","authors":"Bachir Medrouh, Amine Abdelli, Salem Belkessa, Yacine Ouinten, Malika Brahimi, Ahcène Hakem, Tahar Kernif, Steven M Singer, Hocine Ziam, Anastasios D Tsaousis, Pikka Jokelainen, Giovanni Savini, Edoardo Pasolli","doi":"10.1080/01652176.2024.2396118","DOIUrl":"10.1080/01652176.2024.2396118","url":null,"abstract":"Bluetongue (BT) is a vector-borne disease affecting wild and domestic ruminants in many parts of the world. Although bluetongue virus (BTV) is widespread in ungulates in Africa, available epidemiological information on BT in this continent is limited. This systematic review and meta-analysis aimed to estimate the seroprevalence of BTV and summarize information on associated risk factors in domestic ruminants and camels in Africa. Systematic searches were conducted from the inception of the database to November 2022 on PubMed/MEDLINE, ScienceDirect, Web of Science, and Google/Google Scholar. Forty-four eligible publications were identified, published in the range from 1973 to 2020, and statistically analyzed. The pooled overall seroprevalence of BTV was 45.02% (95% confidence interval [CI]: 36.00-54.00%). The pooled seroprevalence was 49.70% (95% CI: 34.50-65.00%) in cattle, 47.00% (95% CI: 29.90-64.50%) in goats, 40.80% (95% CI: 19.60-63.90%) in camels, and 36.30% (95% CI: 29.00-44.90%) in sheep. The pooled seroprevalence decreased after 1990 and increased again after 2010. The highest pooled overall seroprevalence was found in the southeastern region, and the highest pooled overall seroprevalence was obtained by Competitive Enzyme-Linked Immunosorbent Assay. Finally, the seroprevalence in females (53.30%, 95% CI: 34.80-71.00%) was significantly higher than in males (28.10%, 95% CI: 17.40-40.30%) (p < 0.05). We showed that antibodies against BTV were common in African ruminants and camels. Monitoring the seroprevalence of BTV, as well as systematic and continuous surveillance of the Culicoides population, are encouraged to prevent and control the spread of BT.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-12"},"PeriodicalIF":7.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11370698/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142114541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transboundary viral diseases of pigs, poultry and ruminants in Southeast Asia: a systematic review. 东南亚猪、家禽和反刍动物跨境病毒性疾病：系统综述。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-08-30 DOI: 10.1080/01652176.2024.2397796

Thi Ngan Mai, Thanh Trung Nguyen, Sinh Dang-Xuan, Hung Nguyen-Viet, Fred Unger, Hu Suk Lee

{"title":"Transboundary viral diseases of pigs, poultry and ruminants in Southeast Asia: a systematic review.","authors":"Thi Ngan Mai, Thanh Trung Nguyen, Sinh Dang-Xuan, Hung Nguyen-Viet, Fred Unger, Hu Suk Lee","doi":"10.1080/01652176.2024.2397796","DOIUrl":"10.1080/01652176.2024.2397796","url":null,"abstract":"Livestock is a strategic part of the small-farm economy in Southeast Asia's society, providing food income, clothing, fertilizer, and draught power. However, incidences or outbreaks of transboundary animal diseases (TADs) are due to converging factors such as the natural hazards' aftermath, climate change, deforestation, urban growth, changing production conditions, and market chains. Therefore, this affects productivity and impacts farmers' livelihoods with small holdings. The literature review was carried out to understand the current situation of TADs in Southeast Asia, identifying knowledge gaps to provide actions for disease control and prevention in the region. We have attempted to summarise the scientific literature in English on the prevalence data of TADs in Southeast Asia between 2011 and March 2022. Relatively few studies evaluated the distribution of TAD, where most of the studies focused on diseases that are important for international trade, such as avian influenza (AI), African swine fever (ASF), classical swine fever (CSF), foot-and-mouth disease (FMD) and Newcastle disease (ND). Traditional production systems have received little attention in such studies as they belonged to mainly smallholders. The outbreaks of ASF and lumpy skin disease (LSD) in 2019 resulted in increased research activity between 2019-2022, while the other TADs were ignored in this period. For new emerging TADs diseases such as ASF and LSD, there is only information about the first detection without prevalence information. Therefore, further epidemiological investigations are necessary to reduce the gaps in disease surveillance reporting systems and support the prevention and reduction of further outbreaks.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"13-25"},"PeriodicalIF":7.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11370669/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142114543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Circulating nucleosomes as a potential cancer biomarker in dogs with splenic nodular lesions. 循环核小体是患脾结节病狗的潜在癌症生物标志物。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-09-03 DOI: 10.1080/01652176.2024.2399648

Sara Meazzi, Valeria Martini, Laura Marconato, Marina Aralla, Luca Licenziato, Matteo Olimpo, Paola Roccabianca, Silvia Sabattini, Alessandra Ubiali, Riccardo Zaccone, Luca Aresu

{"title":"Circulating nucleosomes as a potential cancer biomarker in dogs with splenic nodular lesions.","authors":"Sara Meazzi, Valeria Martini, Laura Marconato, Marina Aralla, Luca Licenziato, Matteo Olimpo, Paola Roccabianca, Silvia Sabattini, Alessandra Ubiali, Riccardo Zaccone, Luca Aresu","doi":"10.1080/01652176.2024.2399648","DOIUrl":"10.1080/01652176.2024.2399648","url":null,"abstract":"Splenic nodular lesions in dogs can be either benign or malignant. They might be discovered incidentally or, in case of rupture, they may lead to hemoabdomen. Nevertheless, splenectomy followed by histopathology is essential for diagnosis and to prevent rupture. Yet, this invasive procedure might be postponed for dogs with benign splenic nodular lesions. Conversely, owners may opt for euthanasia over surgery for malignancies with poor prognosis like hemangiosarcoma. Thus, anticipating diagnosis with non-invasive biomarkers is crucial for proper patient management. In this prospective study, plasma samples were collected from 66 dogs with histologically confirmed splenic nodular lesions. A canine-specific ELISA kit was applied to assess nucleosome concentration, with histopathology of the spleen serving as the gold standard. Nucleosome concentration was found to be significantly higher in dogs with malignant splenic nodular lesions, particularly in those with hemangiosarcoma and other malignancies. The presence of hemoabdomen, more prevalent in dogs with splenic malignancy, also resulted in increased plasmatic nucleosome concentrations. Plasma nucleosomes could serve as a biomarker for detecting malignant splenic nodular lesions in dogs. More research is needed to understand how nucleosome concentration relate to disease stage and prognosis in dogs with hemangiosarcoma.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-7"},"PeriodicalIF":7.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11373370/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142121120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing in vitro stability of remdesivir (GS-5734) and conversion to GS-441524 in feline plasma and whole blood. 评估雷米替韦（GS-5734）在猫血浆和全血中的体外稳定性以及向 GS-441524 的转化。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-01-30 DOI: 10.1080/01652176.2024.2305731

Sally J Coggins, Benjamin Kimble, Richard Malik, Mary F Thompson, Jacqueline M Norris, Merran Govendir

{"title":"Assessing in vitro stability of remdesivir (GS-5734) and conversion to GS-441524 in feline plasma and whole blood.","authors":"Sally J Coggins, Benjamin Kimble, Richard Malik, Mary F Thompson, Jacqueline M Norris, Merran Govendir","doi":"10.1080/01652176.2024.2305731","DOIUrl":"10.1080/01652176.2024.2305731","url":null,"abstract":"Feline infectious peritonitis (FIP) is a potentially fatal coronavirus-driven disease of cats. Treatment with nucleoside analogue GS-441524 and or prodrug remdesivir (RDV) have produced remission in both experimentally induced and naturally occurring FIP, yet information regarding metabolism of RDV into GS-441524 in cats is scarce. This study assessed possible phase I metabolism of RDV in cats, utilising an in vitro feline microsome model with in vitro t1/2 and in vitro Clint calculated using the substrate depletion method. A previously validated high-performance liquid chromatography (HPLC) fluorescence method was utilised for detection and analysis of RDV and GS-441524. Qualitative yield of RDV and intermediate metabolite GS-441524 were determined following microsome incubation, then compared to whole blood and plasma incubations. In vitro microsome incubation resulted in rapid depletion of RDV, though it did not appear to resemble a conventional phase I-dependent reaction in cats, as it is in humans and dogs. Depletion of RDV into GS-441524 was demonstrated in whole blood in vitro, suggesting cats convert RDV to GS-441524, likely via blood esterases, as observed in mice and rats. RDV metabolism is unlikely to be impacted by impaired liver function in cats. Furthermore, as RDV depletes within minutes, whereas GS-441524 is very stable, whole blood or plasma GS-441524 concentrations, rather than plasma RDV concentrations, are more appropriate for therapeutic drug monitoring (TDM) in cats receiving RDV.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-9"},"PeriodicalIF":7.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10829815/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139576181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0