Veterinary Quarterly最新文献

Effects of ex situ conservation on commensal bacteria of crocodile lizard and conservation implications. 迁地保护对鳄鱼蜥蜴共生细菌的影响及其保护意义。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-02-10 DOI: 10.1080/01652176.2025.2463704

Haiying Jiang, Mei Lv, Tengfei He, Mujiao Xie, Zhiwen Zhao, Jiasong He, Shuyi Luo, Yide Guo, Jinping Chen

{"title":"Effects of ex situ conservation on commensal bacteria of crocodile lizard and conservation implications.","authors":"Haiying Jiang, Mei Lv, Tengfei He, Mujiao Xie, Zhiwen Zhao, Jiasong He, Shuyi Luo, Yide Guo, Jinping Chen","doi":"10.1080/01652176.2025.2463704","DOIUrl":"10.1080/01652176.2025.2463704","url":null,"abstract":"Ex situ conservation is an important wildlife conservation strategy, but endangered wildlife in captivity often exhibit high disease rates. Commensal microorganisms are vital for homeostasis, immunity, and linked to diseases. This study analyzed the structure, assembly, variations of the symbiotic microbiota of the endangered crocodile lizard, and their relationship with environment, as well as the effects of captivity on them, to explore why captive reptiles face high dermatosis rates. Results showed that the reptile's microbiota significantly differ from that of its habitat, demonstrating niche specificity. While species richness among organs showed no significant differences, microbial diversity varied considerably. Skin microbiota showed no site-specific clustering. The assembly of skin, oral, and intestinal bacterial communities was dominated by homogeneous selection. The gut and oral bacterial networks were resilient to disturbances, while the skin bacterial network was sensitive. Captivity primarily affected the skin microbiota, reducing its diversity and stability, thereby increasing disease risk, and these effects were not solely attributable to environmental changes. These findings suggested that skin microbial changes in captive reptiles may be responsible for their increased susceptibility to dermatosis in ex situ conservation. This study underscored the importance of understanding reptile-associated microbes for effective conservation strategies and offers potential solutions.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-14"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11816626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143392500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The canine blood-brain barrier in health and disease: focus on brain protection. 犬血脑屏障的健康与疾病：重点关注脑保护。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-01-10 DOI: 10.1080/01652176.2025.2450041

Dimitri Bassalo, Stephen G Matthews, Enrrico Bloise

{"title":"The canine blood-brain barrier in health and disease: focus on brain protection.","authors":"Dimitri Bassalo, Stephen G Matthews, Enrrico Bloise","doi":"10.1080/01652176.2025.2450041","DOIUrl":"10.1080/01652176.2025.2450041","url":null,"abstract":"This review examines the role of the canine blood-brain barrier (BBB) in health and disease, focusing on the impact of the multidrug resistance (MDR) transporter P-glycoprotein (P-gp) encoded by the ABCB1/MDR1 gene. The BBB is critical in maintaining central nervous system homeostasis and brain protection against xenobiotics and environmental drugs that may be circulating in the blood stream. We revise key anatomical, histological and functional aspects of the canine BBB and examine the role of the ABCB1/MDR1 gene mutation in specific dog breeds that exhibit reduced P-gp activity and disrupted drug brain pharmacokinetics. The review also covers factors that may disrupt the canine BBB, including the actions of aging, canine cognitive dysfunction, epilepsy, inflammation, infection, traumatic brain injury, among others. We highlight the critical importance of this barrier in maintaining central nervous system homeostasis and protecting against xenobiotics and conclude that a number of neurological-related diseases may increase vulnerability of the BBB in the canine species and discuss its profound impacts on canine health.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"12-32"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11727060/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142958392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolomics reveals alterations in gut-derived uremic toxins and tryptophan metabolism in feline chronic kidney disease. 代谢组学揭示了猫慢性肾病中肠道源性尿毒症毒素和色氨酸代谢的改变。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-01-02 DOI: 10.1080/01652176.2024.2447601

Laurens Van Mulders, Ellen Vanden Broecke, Ellen De Paepe, Femke Mortier, Lynn Vanhaecke, Sylvie Daminet

{"title":"Metabolomics reveals alterations in gut-derived uremic toxins and tryptophan metabolism in feline chronic kidney disease.","authors":"Laurens Van Mulders, Ellen Vanden Broecke, Ellen De Paepe, Femke Mortier, Lynn Vanhaecke, Sylvie Daminet","doi":"10.1080/01652176.2024.2447601","DOIUrl":"https://doi.org/10.1080/01652176.2024.2447601","url":null,"abstract":"Chronic Kidney Disease (CKD) is one of the most common conditions affecting felines, yet the metabolic alterations underlying its pathophysiology remain poorly understood, hindering progress in identifying biomarkers and therapeutic targets. This study aimed to provide a comprehensive view of metabolic changes in feline CKD across conserved biochemical pathways and evaluate their progression throughout the disease continuum. Using a multi-biomatrix high-throughput metabolomics approach, serum and urine samples from CKD-affected cats (n = 94) and healthy controls (n = 84) were analyzed with ultra-high-performance liquid chromatography-high-resolution mass spectrometry. Significant disruptions were detected in tryptophan (indole, kynurenine, serotonin), tyrosine, and carnitine metabolism, as well as in the urea cycle. Circulating gut-derived uremic toxins, including indoxyl-sulfate, p-cresyl-sulfate, and trimethylamine-N-oxide, were markedly increased, primarily due to impaired renal excretion. However, alternative mechanisms, such as enhanced bacterial formation from dietary precursors like tryptophan, tyrosine, carnitine, and betaine, could not be ruled out. Overall, the findings suggest that metabolic disturbances in feline CKD are largely driven by the accumulation of gut-derived uremic toxins derived from precursors highly abundant in the feline diet. These insights may link the strict carnivorous nature of felines to CKD pathophysiology and highlight potential avenues for studying preventive or therapeutic interventions.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-15"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142916206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Machine learning based diagnostics of veterinary cancer on ultrasound and optical imaging data. 基于超声和光学成像数据的基于机器学习的兽医癌症诊断。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-05-30 DOI: 10.1080/01652176.2025.2510486

Martynas Maciulevičius, Greta Rupšytė, Renaldas Raišutis, Mindaugas Tamošiūnas

{"title":"Machine learning based diagnostics of veterinary cancer on ultrasound and optical imaging data.","authors":"Martynas Maciulevičius, Greta Rupšytė, Renaldas Raišutis, Mindaugas Tamošiūnas","doi":"10.1080/01652176.2025.2510486","DOIUrl":"https://doi.org/10.1080/01652176.2025.2510486","url":null,"abstract":"Study advances current diagnostic efficiency of canine/feline (sub-)cutaneous tumors using machine learning and multimodal imaging data. White light (WL), fluorescence (FL) and ultrasound (US) imaging were combined into hybrid approaches to differentiate between malignant mastocytomas, soft tissue sarcomas and benign lipomas. Support Vector Machine and Ensemble classifiers were optimized via sequential feature selection. US radio-frequency signals were quantitatively analyzed to derive the colormaps of six US estimates, corresponding to spectral and temporal domains of the acoustic field. This resulted in the quantification of 72 morphological features for US; as well as 24 and 12 - for WL and FL data, respectively. Resulting classification efficiency for mastocytoma and sarcoma using US data was >75%; US+FL - 75-80%; US+WL - 85-90% and US+OPTICS - 90-95%. ∼100% classification efficiency was achieved for the differentiation between benign and malignant tumors even using single WL feature for Ensemble classifier. US features, resulting in inferior classification efficiency, were competitive to superior optical, as they were selected during optimization to be added to or replace optical counterparts. Additional tissue differentiation was performed on z-stacks of US colormaps, obtained using 3D arrays of US radio-frequency signals. This resulted in ∼70% differentiation efficiency for mastocytoma and sarcoma as well as >95% for benign and malignant tissues. The obtained additional metric of classification efficiency provides complementary diagnostic support, which for Support Vector Machine can be expressed as: 90.3 ± 1.9% (US+WL)×71.2 ± 0.6% (USDepth Profile). This hybrid criterion adds robustness to diagnostic model and may be very beneficial to characterize heterogeneous tissues.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-19"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144188514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a differentiating of infected from vaccinated animal (DIVA) ELISA to detect antibodies against Senecavirus A in pigs using two expression systems of non-structural proteins. 利用两种非结构蛋白表达系统，建立猪塞内卡病毒a抗体的感染与接种动物区分（DIVA） ELISA检测方法。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-01-08 DOI: 10.1080/01652176.2024.2449082

Parin Watcharavongtip, Patumporn Jermsutjarit, Angkana Tantituvanont, Dachrit Nilubol

{"title":"Development of a differentiating of infected from vaccinated animal (DIVA) ELISA to detect antibodies against Senecavirus A in pigs using two expression systems of non-structural proteins.","authors":"Parin Watcharavongtip, Patumporn Jermsutjarit, Angkana Tantituvanont, Dachrit Nilubol","doi":"10.1080/01652176.2024.2449082","DOIUrl":"10.1080/01652176.2024.2449082","url":null,"abstract":"Senecavirus A (SVA) is the causative agent associated with porcine idiopathic vesicular disease (PIVD), a condition indistinguishable from other foreign vesicular diseases affecting pigs. This complicates differential diagnosis and impacts the global swine industry. A diagnostic ELISA based on a non-structural viral protein has been developed, capable of distinguishing infected from vaccinated animals (DIVA). Different expression systems (eukaryotic and prokaryotic) were used to express recombinant proteins. The baculovirus-expressed SVA 3AB DIVA ELISA demonstrated a sensitivity of 96.67% and specificity of 96.67%. In contrast, the E. coli-expressed SVA 3AB DIVA ELISA achieved 100% sensitivity and 93.33% specificity. Both ELISAs strongly correlated with the reference method and showed no cross-reactivity with other pig pathogens. The E. coli system also provided a higher yield of expressed protein than the baculovirus system. These findings indicate that SVA DIVA ELISAs are effective alternatives for detecting SVA antibodies. They can be valuable tools for sero-surveillance and for evaluating immunity status tests to support and approve vaccination programs for pig herds in the future.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-11"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11721756/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142958387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epidemiology and pathobiology of H5Nx highly pathogenic avian influenza in South Korea (2003-2024): a comprehensive review. 韩国H5Nx高致病性禽流感流行病学和病理生物学（2003-2024）：全面回顾

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-05-07 DOI: 10.1080/01652176.2025.2498918

Sun-Hak Lee, Jung-Hoon Kwon, Sungsu Youk, Sang-Won Lee, Dong-Hun Lee, Chang-Seon Song

{"title":"Epidemiology and pathobiology of H5Nx highly pathogenic avian influenza in South Korea (2003-2024): a comprehensive review.","authors":"Sun-Hak Lee, Jung-Hoon Kwon, Sungsu Youk, Sang-Won Lee, Dong-Hun Lee, Chang-Seon Song","doi":"10.1080/01652176.2025.2498918","DOIUrl":"https://doi.org/10.1080/01652176.2025.2498918","url":null,"abstract":"Since their emergence in Guangdong, China, in 1996, Gs/GD H5 highly pathogenic avian influenza viruses (HPAIVs) have diversified into multiple clades, spreading globally through wild bird migrations and causing substantial losses in poultry and wildlife. In South Korea, HPAIVs, including H5N1, H5N8, and H5N6 subtypes, have been repeatedly introduced since 2003. This review examines the epidemiology, genetic characteristics, and pathobiological features of these viruses in South Korea. Outbreaks typically occur between October and December, aligning with the arrival of wintering migratory birds. While outbreaks in poultry farms dominated before 2018, wild bird cases became more prevalent in subsequent years. Seasonal outbreaks in poultry have declined, but large-scale mortality events in wild birds emerged biennially from 2020. Genotypic diversity has increased since 2014 due to reassortment with low pathogenic viruses, with novel genomic traits detected in recent seasons. Infection studies show consistently fatal outcomes in chickens, while high mortality in domestic ducks was observed only with two of the studied strains, despite efficient transmission. Wild bird studies reveal species-specific roles in viral shedding and transmission. This review underscores the dynamic nature of HPAI outbreaks, highlighting the importance of surveillance, biosecurity, and genetic and pathogenicity analyses to mitigate future risks.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"23-38"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12064103/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144057144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro and in vivo study on the effects of nutritive media compared with water on the resuscitation of a live attenuated Salmonella Typhimurium vaccine. 营养培养基与水对鼠伤寒沙门菌减毒活疫苗复苏效果的体外和体内研究。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-04-10 DOI: 10.1080/01652176.2025.2485480

Siyuan Jia, Andrea R McWhorter, Samiullah Khan, Daniel M Andrews, Gregory J Underwood, Kapil K Chousalkar

{"title":"In vitro and in vivo study on the effects of nutritive media compared with water on the resuscitation of a live attenuated Salmonella Typhimurium vaccine.","authors":"Siyuan Jia, Andrea R McWhorter, Samiullah Khan, Daniel M Andrews, Gregory J Underwood, Kapil K Chousalkar","doi":"10.1080/01652176.2025.2485480","DOIUrl":"https://doi.org/10.1080/01652176.2025.2485480","url":null,"abstract":"In this study, a commercially available Salmonella Typhimurium vaccine (Vaxsafe ST) reconstituted in either water or nutrient broth, was tested to understand its effects on the in vitro invasion of Caco-2 cells and its transcriptional regulation post-reconstitution. Over time, the invasiveness of Vaxsafe ST was significantly higher following reconstitution in nutrient broth compared with water. Incubation temperature post-reconstitution did not significantly affect the invasion rate. Transcriptome data showed that Vaxsafe ST reconstituted in nutrient broth upregulated genes involved in the two-component system and flagella activity pathways; however, genes involved in host colonization and invasion were unaltered. Genes involved in host colonization and invasion were downregulated after reconstitution in water. Vaxsafe ST reconstituted in a nutritive diluent improved the metabolic activities of the vaccine. The animal experiment demonstrated that vaccine colonization was significantly higher in caeca compared with ileum irrespective of the diluent. Incubating the vaccine in nutrient broth for 30 min before administration did not significantly increase its colonization or organ invasion in chicks. Overall, the findings support the use of nutritive media for the reconstitution of Vaxsafe ST, warranting further investigation to optimize vaccine performance. This study provides a foundation for future work on vaccine reconstitution strategies.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-10"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11986869/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144059051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of recombinant extracellular enveloped virion protein candidates for the detection of serological responses to lumpy skin disease virus in cattle. 重组细胞外包膜病毒粒子候选蛋白检测牛肿块性皮肤病病毒血清学反应的评价。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-03-19 DOI: 10.1080/01652176.2025.2475989

Kitipong Angsujinda, Phatpimol Kitchanakan, Nabhasbhichayabha Daewang, Lerdchai Chintapitaksakul, Saruda Wanganurakkul, Sudkate Chaiyo, Nanthika Khongchareonporn, Timothy J Mahony, Wanchai Assavalapsakul

{"title":"Evaluation of recombinant extracellular enveloped virion protein candidates for the detection of serological responses to lumpy skin disease virus in cattle.","authors":"Kitipong Angsujinda, Phatpimol Kitchanakan, Nabhasbhichayabha Daewang, Lerdchai Chintapitaksakul, Saruda Wanganurakkul, Sudkate Chaiyo, Nanthika Khongchareonporn, Timothy J Mahony, Wanchai Assavalapsakul","doi":"10.1080/01652176.2025.2475989","DOIUrl":"10.1080/01652176.2025.2475989","url":null,"abstract":"Lumpy skin disease virus (LSDV) is a significant threat to cattle, particularly in countries like Thailand, where outbreaks have necessitated the importation of diagnostic kits and vaccines. This study aimed to evaluate several recombinant extracellular enveloped virion (EEV) protein candidates, including F13L, A33R, A34R, and B5R, for their potential use in serological detection assays for LSDV specific antibodies in cattle. Given the challenges associated with LSDV research, such as its classification as a Class III biological agent in Thailand, gene synthesis was employed to produce these proteins. The recombinant proteins were expressed in a prokaryotic system and analyzed using SDS-PAGE and Western blotting. Among the candidates, F13L demonstrated the highest correlation with the results from a commercially available and validated ELISA, yielding 85.7%, and 75% positive for the infected and vaccinated groups, respectively, identifying it a promising candidate for serosurveillance activities during active LSDV outbreaks. Sequence analysis confirmed a 100% match between the F13L designed from the Neethling type strain 2490 and various Thai LSDV strains from the 2021 outbreaks, underscoring its potential as a conserved diagnostic marker. The availability of recombinant F13L and its reactivity with cattle sera from LSDV infected or vaccinated animals, demonstrated in this study, suggests it could also serve as a potential candidate for vaccine development. The study concludes that recombinant F13L shows great promise for the development of LSDV serological assays, though further optimization and validation are necessary to harness its diagnostic potential.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-13"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11924265/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic insights into Mycobacterium orygis infection-associated pulmonary granulomas reveal multicellular immune networks and tuberculosis biomarkers in cattle. 转录组学对牛羊分枝杆菌感染相关的肺肉芽肿揭示了多细胞免疫网络和结核病生物标志物。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-05-27 DOI: 10.1080/01652176.2025.2509503

Rishi Kumar, Sripratyusha Gandham, Vinay Bhaskar, Manas Ranjan Praharaj, Hemanta Kumar Maity, Uttam Sarkar, Bappaditya Dey

{"title":"Transcriptomic insights into Mycobacterium orygis infection-associated pulmonary granulomas reveal multicellular immune networks and tuberculosis biomarkers in cattle.","authors":"Rishi Kumar, Sripratyusha Gandham, Vinay Bhaskar, Manas Ranjan Praharaj, Hemanta Kumar Maity, Uttam Sarkar, Bappaditya Dey","doi":"10.1080/01652176.2025.2509503","DOIUrl":"10.1080/01652176.2025.2509503","url":null,"abstract":"Mycobacterium orygis, a member of the Mycobacterium tuberculosis complex (MTBC), has emerged as a significant contributor to tuberculosis (TB) in cattle, wildlife, and humans. However, understanding about its pathogenesis and severity is limited, compounded by the lack of reliable TB biomarkers in cattle. This study delves into the comparative pathology and transcriptomic landscape of pulmonary granulomas in cattle naturally infected with M. orygis, using high-throughput RNA sequencing. Histopathological analysis revealed extensive, multistage granulomatous, necrotic, and cavitary lesions, indicative of severe lung pathology induced by M. orygis. Transcriptomic profiling highlighted numerous differentially expressed genes and dysregulated pathways related to immune response modulation and extracellular matrix remodelling. Additionally, cell type enrichment analysis provided insights into the multicellularity of the granulomatous niche, emphasising complex cell-cell interactions within TB granulomas. Via comparative transcriptomics leveraging publicly available bovine and human TB omics datasets, 14 key immunomodulators (SOD2, IL1α/β, IL15, IL18, CCL2/MCP-1, CCL3/MIP-1α, CCL4/MIP-1β, CCL8/MCP-2, CCL20/MIP-3α, CXCL2/MIP-2, CXCL10/IP-10, CXCL11, and IFN-γ) were identified as potential biomarkers for active TB in cattle. These findings significantly advance our understanding of M. orygis pathogenesis in bovine TB and highlight potential targets for the development of diagnostic tools for managing and controlling the disease.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-19"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12120866/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144163345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developing a novel TaqMan qPCR assay for optimizing Salmonella Pullorum detection in chickens. 建立鸡白痢沙门氏菌TaqMan qPCR检测方法。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-01-30 DOI: 10.1080/01652176.2025.2454473

Hao Wang, Xiamei Kang, Longhai Yu, Haijian Wang, Anja Müller, Corinna Kehrenberg, Yan Li, Min Yue

{"title":"Developing a novel TaqMan qPCR assay for optimizing Salmonella Pullorum detection in chickens.","authors":"Hao Wang, Xiamei Kang, Longhai Yu, Haijian Wang, Anja Müller, Corinna Kehrenberg, Yan Li, Min Yue","doi":"10.1080/01652176.2025.2454473","DOIUrl":"10.1080/01652176.2025.2454473","url":null,"abstract":"Salmonella Pullorum, the causative agent of pullorum disease, posing a significant threat to the global production of poultry meat and eggs. However, existing detection methods have substantial limitations in efficiency and accuracy. Herein, we developed a genomic deletion-targeted TaqMan qPCR assay for identification of Salmonella Pullorum, enabling precise differentiation from other Salmonella serovars. The assay's detection limit was 5 copies/μL of plasmid and 4 CFU/μL of bacterial DNA. Furthermore, we collected 676 chicken samples from an established infection model to compare the performance of the TaqMan qPCR assay with traditional bacterial culturing and antibody-based detection approaches. With superior sensitivity and specificity, the newly developed method detected over 80% of positive chickens, significantly outperforming the two conventional methods. Moreover, we proposed a combined framework that incorporates the advantages of TaqMan qPCR assay and antibody detection method, further enhancing the detection rate of positives to 92%. Additionally, to address the frequent aerosol contamination of amplification products in laboratory settings, we devised an easy-to-deploy anti-contamination system based on T7 exonuclease. Overall, the T7 exonuclease-assisted TaqMan qPCR assay will not only upgrade the current detection for pullorum disease, but also exemplify the feasibility of targeting specific genomic deletions for pathogen detection.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-13"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11784030/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143068667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0