Veterinary Quarterly最新文献

The canine blood-brain barrier in health and disease: focus on brain protection. 犬血脑屏障的健康与疾病：重点关注脑保护。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-01-10 DOI: 10.1080/01652176.2025.2450041

Dimitri Bassalo, Stephen G Matthews, Enrrico Bloise

{"title":"The canine blood-brain barrier in health and disease: focus on brain protection.","authors":"Dimitri Bassalo, Stephen G Matthews, Enrrico Bloise","doi":"10.1080/01652176.2025.2450041","DOIUrl":"10.1080/01652176.2025.2450041","url":null,"abstract":"This review examines the role of the canine blood-brain barrier (BBB) in health and disease, focusing on the impact of the multidrug resistance (MDR) transporter P-glycoprotein (P-gp) encoded by the ABCB1/MDR1 gene. The BBB is critical in maintaining central nervous system homeostasis and brain protection against xenobiotics and environmental drugs that may be circulating in the blood stream. We revise key anatomical, histological and functional aspects of the canine BBB and examine the role of the ABCB1/MDR1 gene mutation in specific dog breeds that exhibit reduced P-gp activity and disrupted drug brain pharmacokinetics. The review also covers factors that may disrupt the canine BBB, including the actions of aging, canine cognitive dysfunction, epilepsy, inflammation, infection, traumatic brain injury, among others. We highlight the critical importance of this barrier in maintaining central nervous system homeostasis and protecting against xenobiotics and conclude that a number of neurological-related diseases may increase vulnerability of the BBB in the canine species and discuss its profound impacts on canine health.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"12-32"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11727060/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142958392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolomics reveals alterations in gut-derived uremic toxins and tryptophan metabolism in feline chronic kidney disease. 代谢组学揭示了猫慢性肾病中肠道源性尿毒症毒素和色氨酸代谢的改变。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-01-02 DOI: 10.1080/01652176.2024.2447601

Laurens Van Mulders, Ellen Vanden Broecke, Ellen De Paepe, Femke Mortier, Lynn Vanhaecke, Sylvie Daminet

{"title":"Metabolomics reveals alterations in gut-derived uremic toxins and tryptophan metabolism in feline chronic kidney disease.","authors":"Laurens Van Mulders, Ellen Vanden Broecke, Ellen De Paepe, Femke Mortier, Lynn Vanhaecke, Sylvie Daminet","doi":"10.1080/01652176.2024.2447601","DOIUrl":"https://doi.org/10.1080/01652176.2024.2447601","url":null,"abstract":"Chronic Kidney Disease (CKD) is one of the most common conditions affecting felines, yet the metabolic alterations underlying its pathophysiology remain poorly understood, hindering progress in identifying biomarkers and therapeutic targets. This study aimed to provide a comprehensive view of metabolic changes in feline CKD across conserved biochemical pathways and evaluate their progression throughout the disease continuum. Using a multi-biomatrix high-throughput metabolomics approach, serum and urine samples from CKD-affected cats (n = 94) and healthy controls (n = 84) were analyzed with ultra-high-performance liquid chromatography-high-resolution mass spectrometry. Significant disruptions were detected in tryptophan (indole, kynurenine, serotonin), tyrosine, and carnitine metabolism, as well as in the urea cycle. Circulating gut-derived uremic toxins, including indoxyl-sulfate, p-cresyl-sulfate, and trimethylamine-N-oxide, were markedly increased, primarily due to impaired renal excretion. However, alternative mechanisms, such as enhanced bacterial formation from dietary precursors like tryptophan, tyrosine, carnitine, and betaine, could not be ruled out. Overall, the findings suggest that metabolic disturbances in feline CKD are largely driven by the accumulation of gut-derived uremic toxins derived from precursors highly abundant in the feline diet. These insights may link the strict carnivorous nature of felines to CKD pathophysiology and highlight potential avenues for studying preventive or therapeutic interventions.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-15"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142916206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of ex situ conservation on commensal bacteria of crocodile lizard and conservation implications.

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-02-10 DOI: 10.1080/01652176.2025.2463704

Haiying Jiang, Mei Lv, Tengfei He, Mujiao Xie, Zhiwen Zhao, Jiasong He, Shuyi Luo, Yide Guo, Jinping Chen

{"title":"Effects of ex situ conservation on commensal bacteria of crocodile lizard and conservation implications.","authors":"Haiying Jiang, Mei Lv, Tengfei He, Mujiao Xie, Zhiwen Zhao, Jiasong He, Shuyi Luo, Yide Guo, Jinping Chen","doi":"10.1080/01652176.2025.2463704","DOIUrl":"10.1080/01652176.2025.2463704","url":null,"abstract":"Ex situ conservation is an important wildlife conservation strategy, but endangered wildlife in captivity often exhibit high disease rates. Commensal microorganisms are vital for homeostasis, immunity, and linked to diseases. This study analyzed the structure, assembly, variations of the symbiotic microbiota of the endangered crocodile lizard, and their relationship with environment, as well as the effects of captivity on them, to explore why captive reptiles face high dermatosis rates. Results showed that the reptile's microbiota significantly differ from that of its habitat, demonstrating niche specificity. While species richness among organs showed no significant differences, microbial diversity varied considerably. Skin microbiota showed no site-specific clustering. The assembly of skin, oral, and intestinal bacterial communities was dominated by homogeneous selection. The gut and oral bacterial networks were resilient to disturbances, while the skin bacterial network was sensitive. Captivity primarily affected the skin microbiota, reducing its diversity and stability, thereby increasing disease risk, and these effects were not solely attributable to environmental changes. These findings suggested that skin microbial changes in captive reptiles may be responsible for their increased susceptibility to dermatosis in ex situ conservation. This study underscored the importance of understanding reptile-associated microbes for effective conservation strategies and offers potential solutions.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-14"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11816626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143392500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a differentiating of infected from vaccinated animal (DIVA) ELISA to detect antibodies against Senecavirus A in pigs using two expression systems of non-structural proteins. 利用两种非结构蛋白表达系统，建立猪塞内卡病毒a抗体的感染与接种动物区分（DIVA） ELISA检测方法。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-01-08 DOI: 10.1080/01652176.2024.2449082

Parin Watcharavongtip, Patumporn Jermsutjarit, Angkana Tantituvanont, Dachrit Nilubol

{"title":"Development of a differentiating of infected from vaccinated animal (DIVA) ELISA to detect antibodies against Senecavirus A in pigs using two expression systems of non-structural proteins.","authors":"Parin Watcharavongtip, Patumporn Jermsutjarit, Angkana Tantituvanont, Dachrit Nilubol","doi":"10.1080/01652176.2024.2449082","DOIUrl":"10.1080/01652176.2024.2449082","url":null,"abstract":"Senecavirus A (SVA) is the causative agent associated with porcine idiopathic vesicular disease (PIVD), a condition indistinguishable from other foreign vesicular diseases affecting pigs. This complicates differential diagnosis and impacts the global swine industry. A diagnostic ELISA based on a non-structural viral protein has been developed, capable of distinguishing infected from vaccinated animals (DIVA). Different expression systems (eukaryotic and prokaryotic) were used to express recombinant proteins. The baculovirus-expressed SVA 3AB DIVA ELISA demonstrated a sensitivity of 96.67% and specificity of 96.67%. In contrast, the E. coli-expressed SVA 3AB DIVA ELISA achieved 100% sensitivity and 93.33% specificity. Both ELISAs strongly correlated with the reference method and showed no cross-reactivity with other pig pathogens. The E. coli system also provided a higher yield of expressed protein than the baculovirus system. These findings indicate that SVA DIVA ELISAs are effective alternatives for detecting SVA antibodies. They can be valuable tools for sero-surveillance and for evaluating immunity status tests to support and approve vaccination programs for pig herds in the future.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-11"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11721756/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142958387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of recombinant extracellular enveloped virion protein candidates for the detection of serological responses to lumpy skin disease virus in cattle.

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-03-19 DOI: 10.1080/01652176.2025.2475989

Kitipong Angsujinda, Phatpimol Kitchanakan, Nabhasbhichayabha Daewang, Lerdchai Chintapitaksakul, Saruda Wanganurakkul, Sudkate Chaiyo, Nanthika Khongchareonporn, Timothy J Mahony, Wanchai Assavalapsakul

{"title":"Evaluation of recombinant extracellular enveloped virion protein candidates for the detection of serological responses to lumpy skin disease virus in cattle.","authors":"Kitipong Angsujinda, Phatpimol Kitchanakan, Nabhasbhichayabha Daewang, Lerdchai Chintapitaksakul, Saruda Wanganurakkul, Sudkate Chaiyo, Nanthika Khongchareonporn, Timothy J Mahony, Wanchai Assavalapsakul","doi":"10.1080/01652176.2025.2475989","DOIUrl":"10.1080/01652176.2025.2475989","url":null,"abstract":"Lumpy skin disease virus (LSDV) is a significant threat to cattle, particularly in countries like Thailand, where outbreaks have necessitated the importation of diagnostic kits and vaccines. This study aimed to evaluate several recombinant extracellular enveloped virion (EEV) protein candidates, including F13L, A33R, A34R, and B5R, for their potential use in serological detection assays for LSDV specific antibodies in cattle. Given the challenges associated with LSDV research, such as its classification as a Class III biological agent in Thailand, gene synthesis was employed to produce these proteins. The recombinant proteins were expressed in a prokaryotic system and analyzed using SDS-PAGE and Western blotting. Among the candidates, F13L demonstrated the highest correlation with the results from a commercially available and validated ELISA, yielding 85.7%, and 75% positive for the infected and vaccinated groups, respectively, identifying it a promising candidate for serosurveillance activities during active LSDV outbreaks. Sequence analysis confirmed a 100% match between the F13L designed from the Neethling type strain 2490 and various Thai LSDV strains from the 2021 outbreaks, underscoring its potential as a conserved diagnostic marker. The availability of recombinant F13L and its reactivity with cattle sera from LSDV infected or vaccinated animals, demonstrated in this study, suggests it could also serve as a potential candidate for vaccine development. The study concludes that recombinant F13L shows great promise for the development of LSDV serological assays, though further optimization and validation are necessary to harness its diagnostic potential.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-13"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11924265/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developing a novel TaqMan qPCR assay for optimizing Salmonella Pullorum detection in chickens.

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-01-30 DOI: 10.1080/01652176.2025.2454473

Hao Wang, Xiamei Kang, Longhai Yu, Haijian Wang, Anja Müller, Corinna Kehrenberg, Yan Li, Min Yue

{"title":"Developing a novel TaqMan qPCR assay for optimizing Salmonella Pullorum detection in chickens.","authors":"Hao Wang, Xiamei Kang, Longhai Yu, Haijian Wang, Anja Müller, Corinna Kehrenberg, Yan Li, Min Yue","doi":"10.1080/01652176.2025.2454473","DOIUrl":"10.1080/01652176.2025.2454473","url":null,"abstract":"Salmonella Pullorum, the causative agent of pullorum disease, posing a significant threat to the global production of poultry meat and eggs. However, existing detection methods have substantial limitations in efficiency and accuracy. Herein, we developed a genomic deletion-targeted TaqMan qPCR assay for identification of Salmonella Pullorum, enabling precise differentiation from other Salmonella serovars. The assay's detection limit was 5 copies/μL of plasmid and 4 CFU/μL of bacterial DNA. Furthermore, we collected 676 chicken samples from an established infection model to compare the performance of the TaqMan qPCR assay with traditional bacterial culturing and antibody-based detection approaches. With superior sensitivity and specificity, the newly developed method detected over 80% of positive chickens, significantly outperforming the two conventional methods. Moreover, we proposed a combined framework that incorporates the advantages of TaqMan qPCR assay and antibody detection method, further enhancing the detection rate of positives to 92%. Additionally, to address the frequent aerosol contamination of amplification products in laboratory settings, we devised an easy-to-deploy anti-contamination system based on T7 exonuclease. Overall, the T7 exonuclease-assisted TaqMan qPCR assay will not only upgrade the current detection for pullorum disease, but also exemplify the feasibility of targeting specific genomic deletions for pathogen detection.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-13"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11784030/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143068667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding zoonotic pathogens and risk factors from wildlife in Southeast Asia: a systematic literature review.

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-03-10 DOI: 10.1080/01652176.2025.2475990

Ha Thi Thanh Nguyen, Johanna F Lindahl, Bernard Bett, Hung Nguyen-Viet, Steven Lâm, Thang Nguyen-Tien, Fred Unger, Sinh Dang-Xuan, Thanh Xuan Bui, Hien Thanh Le, Åke Lundkvist, Jiaxin Ling, Hu Suk Lee

{"title":"Understanding zoonotic pathogens and risk factors from wildlife in Southeast Asia: a systematic literature review.","authors":"Ha Thi Thanh Nguyen, Johanna F Lindahl, Bernard Bett, Hung Nguyen-Viet, Steven Lâm, Thang Nguyen-Tien, Fred Unger, Sinh Dang-Xuan, Thanh Xuan Bui, Hien Thanh Le, Åke Lundkvist, Jiaxin Ling, Hu Suk Lee","doi":"10.1080/01652176.2025.2475990","DOIUrl":"10.1080/01652176.2025.2475990","url":null,"abstract":"The COVID-19 pandemic has demonstrated the significance of the human-animal interface in the emergence of zoonotic diseases, with wildlife serving as an important source of infection. A better understanding of the specific pathogens and mechanisms involved is vital to prepare against future outbreaks, especially in Southeast Asia, a hotspot for zoonotic diseases. This paper reviews the published literature on wildlife zoonoses in this region from 2012 to 2022. The results show a diverse range of potential zoonotic pathogens and the widespread occurrence of zoonotic diseases from wildlife. Drivers of zoonotic pathogen spillover include (i) environmental factors (e.g. animal habitat disruption, environmental conditions, exposure to contaminated water/food/soil), (ii) animal factors (e.g. movement patterns, age-related susceptibility), (iii) human factors (e.g. lack of awareness, poor hygiene practices, age, gender and income) and (iv) human-animal-environmental interface factors (e.g. close contact between humans and animals, exposure through visiting animals and presence of vectors). The diverse drivers of zoonoses in Southeast Asia put its communities at risk for infection. To mitigate these risks, global health efforts should consider adopting a One Health approach to foster collaboration across human, animal, and wildlife health sectors. This could involve educating communities on safe animal interactions and improving disease surveillance.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-17"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11894755/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and characterization of ciprofloxacin-resistant Salmonella enterica spp. isolated from food animals during 2010-2023 in South Korea.

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-03-17 DOI: 10.1080/01652176.2025.2473733

Md Sekendar Ali, Hee-Seung Kang, Bo-Youn Moon, Ye-Eun Heo, Min Young Kim, Ji-Hyun Choi, Yu-Jeong Hwang, Ji-In Kim, Yeon-Hee Lee, Jae-Myung Kim, Suk-Kyung Lim

{"title":"Prevalence and characterization of ciprofloxacin-resistant Salmonella enterica spp. isolated from food animals during 2010-2023 in South Korea.","authors":"Md Sekendar Ali, Hee-Seung Kang, Bo-Youn Moon, Ye-Eun Heo, Min Young Kim, Ji-Hyun Choi, Yu-Jeong Hwang, Ji-In Kim, Yeon-Hee Lee, Jae-Myung Kim, Suk-Kyung Lim","doi":"10.1080/01652176.2025.2473733","DOIUrl":"10.1080/01652176.2025.2473733","url":null,"abstract":"We isolated 6,561 Salmonella strains from food animals, cattle (n = 217), pigs (n = 1526), chickens (n = 3942), and ducks (n = 876). Isolates were evaluated for antimicrobial sensitivity, mutations in quinolone resistance determination regions (QRDRs), and plasmid-mediated quinolone resistance (PMQR) genes. Clonal relationship and genetic diversity were assessed by multi-locus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). Overall, 3.1% of isolates exhibited resistance to ciprofloxacin. Commonly identified mutations in QRDRs were S83F, D87N, and D87G in gyrA; T57S and S80I in parC; and L416F in parE. Furthermore, mutations differed by serotypes. In S. Albany, S83F mutation in gyrA and T57S in parC were prevalent, while in S. Kentucky, S83F and D87N in gyrA, T57S and S80I in parC; and in S. Indiana, S83F and D87G in gyrA, T57S and S80R in parC, and L416F in parE were common. Amongst PMQRs, qnrS was mainly observed in S. Albany, aac(6')-Ib-cr in S. Indiana, and qnrB1 in S. Albany. Among STs, ST198 S. Kentucky was predominant, followed by ST292 S. Albany and ST17 S. Indiana. Of 26 pulsotypes, KX1KA1 was mainly identified in S. Kentucky, AX1AA1 in S. Albany, and IX1IA1 in S. Indiana. Taken together, ciprofloxacin-resistant Salmonella can pose health hazards to humans and other animals.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-11"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11915734/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143651699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isatidis root polysaccharides ameliorates post-weaning diarrhea by promoting intestinal health and modulating the gut microbiota in piglets. 伊沙提斯根多糖通过促进仔猪肠道健康和调节肠道微生物群来改善断奶后腹泻。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-01-03 DOI: 10.1080/01652176.2024.2447600

Miao Zhou, Zenghao Yan, Deqin Wang, Chuyuan Li, Leli Wang, Rui Li, Jie Yin, Yulong Yin

{"title":"Isatidis root polysaccharides ameliorates post-weaning diarrhea by promoting intestinal health and modulating the gut microbiota in piglets.","authors":"Miao Zhou, Zenghao Yan, Deqin Wang, Chuyuan Li, Leli Wang, Rui Li, Jie Yin, Yulong Yin","doi":"10.1080/01652176.2024.2447600","DOIUrl":"https://doi.org/10.1080/01652176.2024.2447600","url":null,"abstract":"This study aimed to investigate the effects of dietary isatidis root polysaccharide (IRP) on diarrhea, immunity, and intestinal health in weanling piglets. Forty healthy piglets were randomly assigned to five groups receiving varying dosages of IRP. The findings indicated that different concentrations of IRP significantly reduced diarrhea scores (p < 0.01). Notably, the serum levels of immunoglobulin A and immunoglobulin G increased linearly and quadratically (p < 0.01), while immunoglobulin M also showed a linear increase (p < 0.05) in IRP-fed piglets. The secretory immunoglobulin A levels in ileal contents were significantly higher compared to control piglets (p < 0.01). Key intestinal health parameters, including villus height, villus height-to-crypt depth ratio, and goblet cell numbers, showed linear and quadratic increases in both the jejunum and ileum (p < 0.05), while crypt depth decreased significantly (p < 0.01). Additionally, the expression of IL-10, ZO-1, occludin, and mucin2 was upregulated linearly and quadratically in IRP-fed piglets (p < 0.05). In cultured IPEC-J2 cells, ZO-1 and occludin expression levels significantly increased upon exposure to 400 μg/mL IRP (p < 0.01). Furthermore, the relative abundances of Escherichia coli, Ralstonia pickettii, and Desulfovibrio fairfieldensis decreased linearly with increasing dietary IRP concentration. In conclusion, IRP shows promise as an effective dietary supplement for mitigating diarrhea and enhancing intestinal health in early weaned piglets.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-15"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142928693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Supplemental glucose oxidase as an antibiotic substitute alleviates diarrhea and improves intestinal health in weaned piglets. 添加葡萄糖氧化酶作为抗生素替代品可减轻断奶仔猪腹泻，改善肠道健康。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-01-01 DOI: 10.1080/01652176.2024.2447592

Dan Chen, Jiaming Chen, Zemin Dong, Qingyun Cao, Hui Ye, Dingyuan Feng, Changming Zhang, Jianjun Zuo, Weiwei Wang

{"title":"Supplemental glucose oxidase as an antibiotic substitute alleviates diarrhea and improves intestinal health in weaned piglets.","authors":"Dan Chen, Jiaming Chen, Zemin Dong, Qingyun Cao, Hui Ye, Dingyuan Feng, Changming Zhang, Jianjun Zuo, Weiwei Wang","doi":"10.1080/01652176.2024.2447592","DOIUrl":"https://doi.org/10.1080/01652176.2024.2447592","url":null,"abstract":"Weaning stress-induced diarrhea is a serious issue in pig production. This study aimed to evaluate the potential of glucose oxidase (GOD) as an antibiotic substitute to alleviate diarrhea and improve gut health in weaned piglets. According to a randomized complete block design, 250 piglets weaned around 21 d of age were allocated into 5 groups (5 replicates/group), which received a basal diet without or with supplemental 200 mg/kg antibiotic, 500, 1000 and 2000 U/kg GOD, respectively. Dietary treatments did not affect (p > 0.05) growth performance of piglets. However, supplemental all doses of GOD were equivalent or superior to antibiotic to reduce (p < 0.05) diarrhea as well as increase (p < 0.05) thymus index, hepatic and colonic antioxidant properties. GOD addition at 1000 and 2000 U/kg reduced (p < 0.05) cecal and rectal pH value, respectively. They also displayed similar or superior efficacy to antibiotic in improving (p < 0.05) duodenal and jejunal morphology along with certain tight junction proteins expression of jejunum and colon. Collectively, GOD represents an antibiotic alternative to reduce diarrhea of weaned piglets through associating with ameliorations of intestinal structure and functions.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-9"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142916207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0