Veterinary Quarterly最新文献

{"title":"Multiplexed immunoassay for a serum autoantibody biomarker panel in diagnostic and prognostic prediction of canine mammary tumors.","authors":"Chih-Ching Wu, Chia-Yu Chang, Pei-Yi Chou, Xiu-Ya Chan, Chun-Chueh Huang, Youngsen Yang, Hao-Ping Liu","doi":"10.1080/01652176.2024.2435978","DOIUrl":"10.1080/01652176.2024.2435978","url":null,"abstract":"<p><p>Canine mammary tumor (CMT) is a prevalent and destructive disease often diagnosed at an advanced stage, leading to poor outcomes. Currently, there is a lack of effective biomarkers for early detection and prognostic prediction of CMT. To improve CMT detection, we established a multiplexed immunoassay using a fluorescence bead-based suspension array system to measure serum levels of autoantibodies against four CMT-associated proteins (AGR2, HAPLN1, IGFBP5, and TYMS) in CMT patients. Our data revealed that serum levels of the four autoantibodies (anti-AGR2, anti-HAPLN1, anti-IGFBP5, and anti-TYMS) were significantly elevated in CMT patients (<i>n</i> = 158) compared to healthy individuals (<i>n</i> = 39). Notably, serum levels of anti-AGR2, anti-HAPLN1, and anti-TYMS in the dogs with stage I CMT (<i>n</i> = 56) were higher than those in the healthy group. Using a marker panel consisting of the four autoantibodies for detecting malignant CMT (<i>n</i> = 125) achieved a sensitivity of 50.4% and a specificity of 90%. Furthermore, higher levels of anti-AGR2, anti-HAPLN1, anti-IGFBP5, and anti-TYMS were associated with poorer survival in CMT patients. Collectively, we established a multiplexed immunoassay platform to detect serum autoantibodies and demonstrated that a tailored autoantibody marker panel shows potential clinical applicability for the diagnosis and prognosis of CMT.</p>","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-12"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11626869/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142787679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular identification and biological characterization of <i>Eimeria columbarum</i> from domestic pigeons (<i>Columba livia domestica</i>) in Guangdong, China.","authors":"Xi He, Xue-Mei Fang, Yu-Tong Qiao, Jia-Li Su, Sheng-Qiu Tang, Ya-Biao Weng, Rui-Qing Lin","doi":"10.1080/01652176.2024.2412297","DOIUrl":"https://doi.org/10.1080/01652176.2024.2412297","url":null,"abstract":"<p><p>Pigeon coccidiosis caused by <i>Eimeria</i> spp. is an important veterinary disease with a significant economic impact on the pigeon industry. Preventive measures for <i>Eimeria columbarum</i> in pigeons have been hampered by the lack of extensive genetic, morphological, and biological data on the oocysts. In this study, we examined the prevalence and identity of <i>Eimeria</i> spp. in domestic pigeons from seven cities in Guangdong Province, China. Data show that coccidiosis was prevalent in domestic pigeons in Guangdong Province, with an overall <i>Eimeria</i> spp. detection rate of 73.4%. Five <i>Eimeria</i> species were identified, including <i>E. columbarum</i> (73.4%), <i>Eimeria kapotei</i> (25.6%), <i>Eimeria labbeana</i> (19.6%), <i>Eimeria duculai</i> (19.6%), and <i>Eimeria tropicalis</i> (6.7%). We obtained single oocyst-derived lines of the dominant <i>E. columbarum</i> from fecal specimens. <i>E. columbarum</i> oocysts measured 20.06 ± 0.69 μm × 18.63 ± 1.03 μm, and sporocysts measured 10.29 ± 0.82 μm × 85.38 ± 0.46 μm. In infection experiment using obtained <i>E. columbarum</i> isolates, 60-day-old coccidia-free pigeons exhibited a prepatent period of 105 h and patent period of 9-10 days followed by severe diarrhea, depression, anorexia, and emaciation. Endogenous development of the parasite was observed mainly in the cytoplasm of epithelial cells in the duodenum, jejunum, ileum, and rectum. Two generations of meronts developed on days 3 and 4 after infection, respectively, while gamont and gamete developed on day 5 after infection. The morphological, genetic, and biological data are expected to be useful in elucidating the biological characterization of pigeon coccidiosis to develop measures against the treatment and containment of this disease.</p>","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-11"},"PeriodicalIF":7.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11486315/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142480145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of chronic stress status and quality of life in cats suffering from chronic kidney disease and suspected feline infectious peritonitis based on hair cortisol concentration analysis and a questionnaire.","authors":"Chien-Hui Chen, Chao-Chin Chang, Wei-Che Chen, Ya-Jane Lee","doi":"10.1080/01652176.2024.2379327","DOIUrl":"10.1080/01652176.2024.2379327","url":null,"abstract":"<p><p>Hair cortisol concentration (HCC) and a questionnaire were used as indicators of chronic stress status and quality of life (QoL), respectively, in cats. To date, there has been limited research on the simultaneous application of both indicators in unwell cats. Our aim was to evaluate HCC and questionnaire data obtained from a healthy cat cohort (<i>n</i> = 61) and cat cohorts with either chronic kidney disease (CKD) (<i>n</i> = 78) or suspected feline infectious peritonitis (FIP) (<i>n</i> = 24). Furthermore, we also investigated the correlation between HCC and clinical pathological data. For this study, hair from the abdomen of cats was collected and analyzed for HCC using a commercial ELISA kit. Owners also completed a questionnaire, from which average-item-weighted-impact-scores (AWISs) were calculated. Cats with late-stage-CKD (median, HCC = 330.15 pg/mg, AWIS = -0.43) presented with a significantly higher HCC (<i>p</i> < 0.01) and a significantly lower AWIS (<i>p</i> < 0.01) than cats with early-stage-CKD (HCC = 183.56 pg/mg, AWIS = 1.08). Similarly, there were significant differences in both HCC (<i>p</i> < 0.001) and AWIS (<i>p</i> < 0.001) between cats with suspected FIP (HCC = 896.27 pg/mg, AWIS = -1.97) and healthy cats (HCC = 181.24 pg/mg, AWIS = 1.24). The degree of consistency between the HCC results and the questionnaire results reminds us that the severity of a chronic disease or the presence of a life-threatening disease can significantly increase stress and thus can affect the QoL of cats.</p>","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-9"},"PeriodicalIF":7.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11262211/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141724983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the clinical usefulness of pancreatic alpha amylase as a novel biomarker in dogs with acute pancreatitis: a pilot study.","authors":"Keon Kim, Hee-Hong Kim, Jae-Beom Joo, Ock-Kyu Kim, Sin-Wook Park, Guk-Hyun Suh, Woong-Bin Ro, Chang-Min Lee","doi":"10.1080/01652176.2024.2326007","DOIUrl":"10.1080/01652176.2024.2326007","url":null,"abstract":"<p><p>Pancreatic alpha amylase (P-AMY) is used as a biomarker of acute pancreatitis (AP) in human medicine. To our knowledge, there are no studies evaluating the usefulness of P-AMY in dogs with AP. In this study, we evaluated the diagnostic value of P-AMY, currently not verified in veterinary medicine. The AP group (<i>n</i> = 40) consisted of dogs with AP diagnosed using clinical signs and laboratory examinations, including abnormal canine pancreatic lipase (cPL) concentration, and compatible abdominal ultrasound examination at first presentation. Evaluation of the canine AP severity (CAPS) score was performed. The control group (<i>n</i> = 38) was composed of normal dogs without any abnormalities in clinical findings, blood exams or diagnostic imaging. The correlation of P-AMY with cPL was confirmed by Pearson's correlation analysis (<i>r</i> = 0.564, <i>p</i> < .001). The sensitivity and specificity for the most appropriate cut-off values of P-AMY were recorded similar to the values of DGGR. The dogs with AP and CAPS ≥11 had significantly higher serum P-AMY (<i>p</i> = .016) contrary to DGGR lipase and cPL. Furthermore, there was a significant difference in the median P-AMY dependent on the presence of systemic inflammatory response syndrome (<i>p</i> = .001). P-AMY showed similar level of diagnostic accuracy along with sensitivity and specificity compared to DGGR lipase. In addition, P-AMY showed a significant association with CAPS score, contrary to cPL and DGGR lipase. Along with other biomarkers associated with AP, P-AMY has the potential of usefulness as a supportive diagnostic and prognostic biomarker of AP in dogs.</p>","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-7"},"PeriodicalIF":6.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10949834/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140144546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Live performance, nutrient digestibility, immune response and fecal microbial load modulation in Japanese quails fed a <i>Bacillus</i>-based probiotic alone or combination with xylanase.","authors":"Asad Sultan, Syed Murtaza, Shabana Naz, Ziaul Islam, Abdulwahed Fahad Alrefaei, Rifat Ullah Khan, Samia H Abdelrahman, A Chandrasekaran","doi":"10.1080/01652176.2024.2364641","DOIUrl":"10.1080/01652176.2024.2364641","url":null,"abstract":"<p><p>Animal industry seeks cost-effective solutions to enhance performance and health of domestic animals. This study investigated the effects of supplementing <i>Bacillus</i> spp. probiotics and xylanase on 2000 one-day-old Japanese quails, randomly assigned to four treatment groups (10 replicates). The control group received no supplementation, while the others were supplemented with a <i>Bacillus</i>-based probiotic at 7.5 × 10⁷ cfu/kg of feed, xylanase enzyme (2,000 U/kg) alone or in combination. Quails receiving both probiotic and enzyme exhibited significantly (<i>p</i> < 0.01) higher weekly and overall weight gain, and lower feed conversion ratios compared to the control group. Dressing percentage was higher (<i>p</i> < 0.01), and mortality lower in birds supplemented with a combination of enzyme and probiotic. Antibody titres against infectious bronchitis and infectious bursal disease were significantly (<i>p</i> < 0.01) higher in quails receiving combined probiotic and enzyme supplementation, while titres against Newcastle disease virus were higher (<i>p</i> < 0.01) in groups supplemented with probiotic and enzyme individually or in combination. Additionally, digestibility was significantly (<i>p</i> < 0.01) higher in groups receiving combined enzyme and probiotic supplementation, with higher apparent metabolizable energy compared to the control. The populations of beneficial <i>Lactobacillus</i> increased, while harmful <i>E. coli</i> and <i>Salmonella</i> decreased significantly in quails supplemented with both probiotic and enzyme. In conclusion, supplementing xylanase enzyme and probiotic together in Japanese quails positively influenced growth, nutrient digestibility, immune response, and cecal microbiota.</p>","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-9"},"PeriodicalIF":7.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11195456/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141433305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diet supplementation with hemp (<i>Cannabis sativa L.</i>) inflorescences: effects on quanti-qualitative milk yield and fatty acid profile on grazing dairy goats.","authors":"Ruggero Amato, Marianna Oteri, Biagina Chiofalo, Fabio Zicarelli, Nadia Musco, Fiorella Sarubbi, Severina Pacifico, Marialuisa Formato, Pietro Lombardi, Federica Di Bennardo, Piera Iommelli, Federico Infascelli, Raffaella Tudisco","doi":"10.1080/01652176.2024.2388715","DOIUrl":"10.1080/01652176.2024.2388715","url":null,"abstract":"<p><p>Hemp (<i>Cannabis sativa L</i>.) is an annual plant belonging to the family of Cannabaceae with several varieties characterized by different fatty acid profile, content in flavonoids, polyphenols, and cannabinoid compounds. Hemp is mostly used in livestock nutrition as oil or as protein cake, but not as inflorescences. The aim of this study was to evaluate the effect of dietary hemp inflorescences on milk yield and composition in grazing dairy goats. Twenty Camosciata delle Alpi goats at their 3rd parity and with a mean body weight of 45.2 ± 2.0 kg, immediately after kidding, were equally allocated into two groups (G: Grazing and GH: grazing and hemp). For three months, all goats were fed on a permanent pasture and received 700/head/day of concentrate; diet of group GH was supplemented with 20 g/head/day of hemp inflorescences. Goats' body weight did not change during the trial. Individual milk yield was daily recorded and samples collected every 20 days for chemical composition and fatty acid profile analysis. No significant differences were found for milk yield and chemical composition. Caproic (C6:0) (1.80 <i>vs.</i> 1.74%; <i>p</i> < 0.01) and lauric acids (C12:0) were significantly higher in milk of group GH (4.83 <i>vs.</i> 4.32%; <i>p</i> < 0.01) as well as linoleic (C18:2) (2.04 <i>vs.</i> 1.93%; <i>p</i> < 0.05), adrenic acid (C22:4) (0.046 <i>vs.</i> 0.031%, <i>p</i> < 0.05), omega-6/omega-3 ratio (3.17 <i>vs.</i> 2.93, <i>p</i> < 0.05) and total conjugated linoleic acids (CLAs) (0.435 <i>vs.</i> 0.417%; <i>p</i> < 0.01). The results of this study suggest that the supplementation of grazing goats' diet with hemp inflorescences may enhance the milk nutritional characteristics by increasing its content of CLAs and other beneficial fatty acids.</p>","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-8"},"PeriodicalIF":7.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11318486/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141908219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A meta-analysis of livestock brucellosis prevalence in Indonesia.","authors":"Muhammad Rosyid Ridlo, Morsid Andityas, Roza Azizah Primatika, Handang Widantara, Shih Keng Loong, Dian Meididewi Nuraini","doi":"10.1080/01652176.2024.2390945","DOIUrl":"10.1080/01652176.2024.2390945","url":null,"abstract":"<p><p>Indonesia has a long history of livestock brucellosis, but the overall pooled prevalence remains unclear. This study aims to determine the pooled estimated prevalence of livestock brucellosis in Indonesia using the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines. Five databases were searched and screened using predefined inclusion and exclusion criteria. Data from included studies were extracted and analyzed using a random effects model in R 4.3.0 for pooled estimated prevalence, subgroup meta-analysis, and meta-regression. Publication bias and sensitivity tests were conducted using Egger's test, funnel plot, trim and fill plot, and leave-one-out. The screening process identified 46 included studies, representing 47,057 samples for brucellosis testing. The pooled estimated prevalence for livestock brucellosis was 3.25% (95% CI, 1.81%-5.78%) with high heterogeneity (<i>Q</i> = 2130.91, <i>p</i> = 0, <i>I²</i>=98%). Subgroup meta-analysis indicated no significant difference in the prevalence of livestock brucellosis across the main islands in Indonesia (<i>p</i> = 0.44) and across provinces in Sulawesi Island (<i>p</i> = 0.83), but significant differences were found among provinces in Java (<i>p</i> < 0.01). The subgroup meta-analysis based on animal type showed no significant difference between cattle, small ruminants, and pig brucellosis estimated prevalence (<i>p</i> = 0.26). Between serological tests, no significant difference was found (<i>p</i> = 0.77). Meta-regression showed no significant difference in brucellosis prevalence from 1988-2023. Egger's test and funnel plot showed publication bias. Trim and fill test indicated 21 studies should be added. As most studies were conducted in Java and Sulawesi Islands, caution should be exercised in interpreting the results, emphasizing the necessity of increasing the study of brucellosis in other regions.</p>","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-14"},"PeriodicalIF":7.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11338204/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142005796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First detection of Omicron variant BA.4.1 lineage in dogs, Chile.","authors":"B Agüero, F Berrios, C Pardo-Roa, N Ariyama, B Bennett, R A Medina, V Neira","doi":"10.1080/01652176.2023.2298089","DOIUrl":"10.1080/01652176.2023.2298089","url":null,"abstract":"<p><p>SARS-CoV-2's rapid global spread caused the declaration of COVID-19 as a pandemic in March 2020. Alongside humans, domestic dogs and cats are also susceptible to infection. However, limited reports on pet infections in Chile prompted a comprehensive study to address this knowledge gap. Between March 2021 and March 2023, the study assessed 65 pets (26 dogs and 39 cats) from 33 COVID-19+ households alongside 700 nasal swabs from animals in households with unknown COVID-19 status. Using RT-PCR, nasal, fecal, and environmental samples were analyzed for the virus. In COVID-19+ households, 6.06% tested positive for SARS-CoV-2, belonging to 3 dogs, indicating human-to-pet transmission. Pets from households with unknown COVID-19 status tested negative for the virus. We obtained 2 SARS-CoV-2 genomes from animals, that belonged to Omicron BA.4.1 variant, marking the first report of pets infected with this lineage globally. Phylogenetic analysis showed these sequences clustered with human sequences collected in Chile during the same period when the BA.4.1 variant was prevalent in the country. The prevalence of SARS-CoV-2 in Chilean pets was relatively low, likely due to the country's high human vaccination rate. Our study highlights the importance of upholding and strengthening human vaccination strategies to mitigate the risk of interspecies transmission. It underscores the critical role of the One Health approach in addressing emerging zoonotic diseases, calling for further research on infection dynamics and risk factors for a comprehensive understanding.</p>","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-10"},"PeriodicalIF":6.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10769545/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139089342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parasitic findings on threatened pudu deer from Central Chile accounts first genetic characterization of lice parasitizing <i>P. puda</i> in Chile and the first molecular report of <i>Taenia hydatigena</i> metacestodes in this species.","authors":"Tamara Muñoz-Caro, María Fernanda González, Rodrigo Villalobos, Alejandro Hidalgo","doi":"10.1080/01652176.2024.2302027","DOIUrl":"10.1080/01652176.2024.2302027","url":null,"abstract":"<p><p>Southern pudu (<i>Pudu puda</i>) is a threatened endemic deer of the temperate forests of Chile. In recent years pudu populations rates have decreased mainly due to anthropogenic causes including forest loss and landscape fragmentation. In this context, the parasitic fauna of Chilean pudu has been scarcely investigated. The aim of this study was to determine the parasitic status of rescued pudu <i>n</i> = 13 from its natural habitat in Central Chile (Maule region) during March 2022 and June 2023 by applying morphological, histopathological, and molecular analyses. As result, we report the presence of transmission of parasites from dogs to pudus as showed by the presence of metacestodes of the parasite <i>Taenia hydatigena</i> on omentum, liver, and pleura of pudus during postmortem examinations, being the first molecular report on the presence of this parasite on Chilean pudu. Meanwhile, ectoparasite examinations determined the presence of chewing and sucking lice on pudu exemplars here analysed. Molecular and phylogenetic analysis of lice revealed new insights on <i>Bovicola</i> and <i>Anoplura</i> lice parasitizing <i>P. puda</i> in Chile, equally being the first genetic characterization of lice parasitizing pudu exemplars in Chile. In addition, parasite loads of lice and metacestodes were analysed. However, no statistically significance was observed when comparing environmental and individual traits influence on parasite load variation. Overall, the study area is the northern limit of habitat distribution of this specie in Chile and we here provide novel information on pudu deer parasites, thus making a useful and valuable contribution to the parasitological knowledge on this threatened species.</p>","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-8"},"PeriodicalIF":7.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10802800/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139503169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial resistance and virulence profiling of <i>Staphylococcus pseudintermedius</i> isolated from cats, Bangladesh.","authors":"Eaftekhar Ahmed Rana, Tanvir Ahmad Nizami, Md Sayedul Islam, Subrata Sarker, Hafizar Rahman, Azizul Hoque, Mizanur Rahman","doi":"10.1080/01652176.2024.2326848","DOIUrl":"10.1080/01652176.2024.2326848","url":null,"abstract":"<p><p><i>Staphylococcus pseudintermedius</i> is a significant bacterial pathogen that frequently colonizes different body sites and mucous membranes of pets. The objectives of the cross-sectional study were to estimate the prevalence, antimicrobial resistance pattern, and detection of diverse resistance as well as virulence genes of <i>S. pseudintermedius</i> in cats. A standard bacteriological method, species-specific gene and different antimicrobial resistance as well as virulence genes were confirmed by PCR assay. A total of 233 swab samples were collected from different body sites of 102 cats, among them 146 swabs from 73 healthy cats, and 87 from 29 diseased cats. Overall, prevalence of <i>S. pseudintermedius</i> in cats was 12.01%, while dermatitis and otitis affected cats were 26.08% and 33.33%, respectively. The highest antimicrobial resistance was observed against penicillin (96.42%) followed by streptomycin (85.71%) and erythromycin (78.57%). Moreover, 89.28% of <i>S. pseudintermedius</i> isolates exhibit multi-drug resistance (MDR) (≥ 3 classes' antimicrobial resistant). In addition, 17.86% isolates harbored the <i>mec</i>A gene; thus, were classified as methicillin-resistant <i>S. pseudintermedius</i> (MRSP). Furthermore, the erythromycin resistance genes <i>erm</i>A and <i>erm</i>B were harbored by 25% and 10.71% of isolates, while 42.86% and 17.86% of isolates carried <i>tet</i>K and <i>tet</i>L (tetracycline resistance) genes, respectively. In virulence profiling, 32.14% (<i>sea</i>) and 10.71% (<i>seb</i>) of isolates were found positive for enterotoxin genes, whereas, the toxic shock syndrome toxin-1 (<i>tst-</i>1) gene and the Panton-Valentine leukocidin gene (<i>pvl</i>) were detected in 25% and 14.29% of isolates, respectively. To our knowledge, this is the first report of cats in Bangladesh for MDR <i>S. pseudintermedius</i>, MRSP, and their virulence profiling.</p>","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-11"},"PeriodicalIF":6.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10946260/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140133198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}