Veterinary Quarterly最新文献

{"title":"High-resolution melting analysis for simultaneous detection and discrimination between wild-type and vaccine strains of feline calicivirus.","authors":"Kannika Phongroop, Jatuporn Rattanasrisomporn, Sahatchai Tangtrongsup, Anudep Rungsipipat, Chutchai Piewbang, Somporn Techangamsuwan","doi":"10.1080/01652176.2023.2272188","DOIUrl":"10.1080/01652176.2023.2272188","url":null,"abstract":"<p><p>High-resolution melting (HRM) analysis, a post-polymerase chain reaction (PCR) application in a single closed tube, is the straightforward method for simultaneous detection, genotyping, and mutation scanning, enabling more significant dynamic detection and sequencing-free turnaround time. This study aimed to establish a combined reverse-transcription quantitative PCR and HRM (RT-qPCR-HRM) assay for diagnosing and genotyping feline calicivirus (FCV). This developed method was validated with constructed FCV plasmids, clinical swab samples from living cats, fresh-frozen lung tissues from necropsied cats, and four available FCV vaccines. We performed RT-qPCR to amplify a 99-base pair sequence, targeting a segment between open reading frame (ORF) 1 and ORF2. Subsequently, the HRM assay was promptly applied using Rotor-Gene Q® Software. The results significantly revealed simultaneous detection and genetic discrimination between commercially available FCV vaccine strains, wild-type Thai FCV strains, and VS-FCV strains within a single PCR reaction. There was no cross-reactivity with other feline common viruses, including feline herpesvirus-1, feline coronavirus, feline leukemia virus, feline immunodeficiency virus, and feline morbillivirus. The detection limit of the assay was 6.18 × 10¹ copies/µl. This study, therefore, is the first demonstration of the uses and benefits of the RT-qPCR-HRM assay for FCV detection and strain differentiation in naturally infected cats.</p>","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":" ","pages":"1-12"},"PeriodicalIF":6.4,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11003490/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49684806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular surveillance of potential SARS-CoV-2 reservoir hosts in wildlife rehabilitation centers.","authors":"Juan Mena, Christian Hidalgo, Daniela Estay-Olea, Nicole Sallaberry-Pincheira, Antonella Bacigalupo, André V Rubio, Diego Peñaloza, Carolina Sánchez, Javiera Gómez-Adaros, Valeria Olmos, Javier Cabello, Kendra Ivelic, María José Abarca, Diego Ramírez-Álvarez, Marisol Torregrosa Rocabado, Natalia Durán Castro, Martina Carreño, Gabriela Gómez, Pedro E Cattan, Galia Ramírez-Toloza, Sofía Robbiano, Carla Marchese, Eduardo Raffo, Paulina Stowhas, Gonzalo Medina-Vogel, Carlos Landaeta-Aqueveque, René Ortega, Etienne Waleckx, Daniel Gónzalez-Acuña, Gemma Rojo","doi":"10.1080/01652176.2023.2164909","DOIUrl":"10.1080/01652176.2023.2164909","url":null,"abstract":"<p><strong>Background: </strong>The COVID-19 pandemic, caused by SARS-CoV-2 infection, has become the most devastating zoonotic event in recent times, with negative impacts on both human and animal welfare as well as on the global economy. Although SARS-CoV-2 is considered a human virus, it likely emerged from animals, and it can infect both domestic and wild animals. This constitutes a risk for human and animal health including wildlife with evidence of SARS-CoV-2 horizontal transmission back and forth between humans and wild animals.</p><p><strong>Aim: </strong>Molecular surveillance in different wildlife rehabilitation centers and wildlife associated institutions in Chile, which are critical points of animal-human interaction and wildlife conservation, especially since the aim of wildlife rehabilitation centers is to reintroduce animals to their original habitat.</p><p><strong>Materials and methods: </strong>The survey was conducted in six WRCs and three wildlife associated institutions. A total of 185 samples were obtained from 83 individuals belonging to 15 different species, including vulnerable and endangered species. Each specimen was sampled with two different swabs: one oropharyngeal or nasopharyngeal according to the nostril diameter, and/or a second rectal sample. RNA was extracted from the samples and two different molecular assays were performed: first, a conventional RT-PCR with pan-coronavirus primers and a second SARS-CoV-2 qPCR targeting the N and S genes.</p><p><strong>Results: </strong>All 185 samples were negative for SARS-CoV-2.</p><p><strong>Clinical relevance: </strong>This study constitutes the first report on the surveillance of SARS-CoV-2 from wildlife treated in rehabilitation centers in Chile, and supports the biosafety procedures adopted in those centers.</p>","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"43 1","pages":"1-10"},"PeriodicalIF":7.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9858396/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10628890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms of circovirus immunosuppression and pathogenesis with a focus on porcine circovirus 2: a review.","authors":"Enikő Fehér, Ferenc Jakab, Krisztián Bányai","doi":"10.1080/01652176.2023.2234430","DOIUrl":"10.1080/01652176.2023.2234430","url":null,"abstract":"<p><p>Certain pathogens, due to their adverse effects on the immune reaction, aggravate the course of concomitant heterologous infections. Here we summarize mechanisms by which circoviruses, including the most studied porcine circovirus 2, and other mammalian and avian circoviruses, trigger their own replication and confound the hosts' immune response. At different stages of infection, from latent state to disease induction, these viruses markedly influence the cellular signaling pathways. Circoviruses have been found to interfere with interferon and proinflammatory cytokine producing and responsive pathways. Apoptotic processes, altered cellular transport and constraint of the mitotic phase all support the viral replication. The cytokine imbalance and lymphocyte depletion, thus the impaired immunity, favors invasion of super- or co-infecting agents, which in concert with circoviruses induce illnesses with increased severity. The information summarized in this review point out the diversity of host and viral factors involved in the mechanisms of disease progression during circovirus infections.</p>","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"43 1","pages":"1-18"},"PeriodicalIF":7.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/84/25/TVEQ_43_2234430.PMC10367577.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9873617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel pathologic findings and viral antigen distribution in cattle and buffalo calves naturally infected with Foot-and-Mouth disease virus.","authors":"Monalisa Sahoo, Rajendra Singh, Pawan Kumar, Asok Kumar Mariappan, Palanivelu Munnuswamy, Karampal Singh, Saminathan Mani, Kuldeep Dhama, Ganesh Kondabattula, Tareni Das, Jigarji Chaturji Thakor, Gayatri Kashyap, Nihar Ranjan Sahoo","doi":"10.1080/01652176.2023.2260435","DOIUrl":"10.1080/01652176.2023.2260435","url":null,"abstract":"<p><p>The Foot-and-Mouth disease is highly contagious acute viral disease of livestock inflicting huge economic loss to the farmers. The limited knowledge regarding the pathological lesions vis-a-vis distribution of the FMDV in lesser explored endocrine glands and important vital organs other than the target organs of infected calves prompted us to take the present investigation to have detailed insight into the pathogenesis. The systematic necropsy of 37 dead calves (cattle-28 and buffalo-9) was conducted, and thin representative tissue pieces from the affected organs were collected in 10% neutral buffered formalin (NBF) for pathological and immunohistochemical investigations. The genomic detection and its serotyping were done by RT-PCR and multiplex-PCR, respectively. Necropsy examination in all cases showed myocardial lesions resembling 'tigroid heart appearance'. Other organ specific lesions include vesiculo-ulcerative stomatitis, edema of the lungs, petechial hemorrhages, edema of the endocrines, and gastroenteritis. Histopathological examination showed varying sizes of vesicles and ulcerations in stratified squamous epithelium of the tongue, acute necrotizing myocarditis, lymphoid depletion in lymphoid tissues, hepatitis, pancreatitis, thymic hyperplasia, thyroiditis, adrenitis, and enteritis. Positive immunolabeling for viral antigens was observed in endocrine glands, lymphoid organs, lungs, liver, kidneys, and intestine, in addition to other typical locations. The thyroid, adrenal glands, and pancreas, in addition to the tongue and heart, are the tissue of choice for sampling in the field during epidemics. Further, the viral genome and serotype A was confirmed in the affected tissues. This study provides insights into novel tissue tropism and pathogenesis in young calves naturally infected with FMDV.</p>","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"43 1","pages":"1-13"},"PeriodicalIF":6.4,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/cf/7c/TVEQ_43_2260435.PMC10548843.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41138821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and characterization of mouse anti-canine PD-L1 monoclonal antibodies and their expression in canine tumors by immunohistochemistry <i>in vitro</i>.","authors":"Sirintra Sirivisoot, Chatikorn Boonkrai, Tossapon Wongtangprasert, Tanapati Phakham, Phijitra Muanwein, Trairak Pisitkun, Chenphop Sawangmake, Araya Radtanakatikanon, Anudep Rungsipipat","doi":"10.1080/01652176.2023.2240380","DOIUrl":"10.1080/01652176.2023.2240380","url":null,"abstract":"<p><p>Immune escape is the hallmark of carcinogenesis. This widely known mechanism is the overexpression of immune checkpoint ligands, such as programmed cell death protein 1 and programmed death-ligand 1 (PD-1/PD-L1), leading to T cell anergy. Therefore, cancer immunotherapy with specific binding to these receptors has been developed to treat human cancers. Due to the lack of cross-reactivity of these antibodies in dogs, a specific canine PD-1/PD-L1 antibody is required. The aim of this study is to develop mouse anti-canine PD-L1 (cPD-L1) monoclonal antibodies and characterize their <i>in vitro</i> properties. Six mice were immunized with recombinant cPD-L1 with a fusion of human Fc tag. The hybridoma clones that successfully generated anti-cPD-L1 antibodies and had neutralizing activity were selected for monoclonal antibody production. Antibody properties were tested by immunosorbent assay, surface plasmon resonance, and immunohistochemistry. Four hybridomas were effectively bound and blocked to recombinant cPD-L1 and cPD-1-His-protein, respectively. Candidate mouse monoclonal antibodies worked efficiently on formalin-fixed paraffin-embedded tissues of canine cancers, including cutaneous T-cell lymphomas, mammary carcinomas, soft tissue sarcomas, squamous cell carcinomas, and malignant melanomas. However, functional assays of these anti-cPD-L1 antibodies need further investigation to prove their abilities as therapeutic drugs in dogs as well as their applications as prognostic markers.</p>","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"43 1","pages":"1-9"},"PeriodicalIF":6.4,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2e/af/TVEQ_43_2240380.PMC10388796.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10276070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Faecal metabolome responses to an altered dietary protein:carbohydrate ratio in adult dogs.","authors":"Yang Lyu,&nbsp;Jia Xu,&nbsp;Fien Verdoodt,&nbsp;Lynn Vanhaecke,&nbsp;Lieselot Y Hemeryck,&nbsp;Myriam Hesta","doi":"10.1080/01652176.2023.2273891","DOIUrl":"10.1080/01652176.2023.2273891","url":null,"abstract":"<p><p>High-protein diets may aid weight loss and weight maintenance programs in both humans and dogs, although the effect of dietary protein levels on gut metabolism and functionality has not been studied in depth. The current study aimed to investigate the effect of an altered dietary protein:carbohydrate ratio on gut function in adult dogs by means of faecal metabolomic fingerprinting. More specifically, functional metabolic differences in dogs fed a high-protein/low-carbohydrate (HPLC) <i>vs.</i> low-protein/high-carbohydrate (LPHC) diet were studied by equally allocating twelve clinically healthy (6 lean and 6 obese) Beagles into two groups in a cross-over design, with each group receiving two isocaloric diets for four weeks. The faecal metabolome revealed that different protein:carbohydrate ratio can influence host and/or gut microbiome metabolism and function, while no effect was observed on the body condition. Targeted analysis demonstrated that the HPLC diet significantly increased the concentration of indole, spermidine, and pipecolinic acid and decreased the concentration of azelaic acid, D-fructose, mannose, and galactose (<i>p</i> < 0.05). Multivariate modelling (OPLS-DA) of the untargeted faecal metabolome revealed distinctly different metabolomic profiles following the HPLC <i>vs.</i> LPHC diet, with 18 altered pathways. The HPLC diet influenced amino acid and lipid metabolism, potentially promoting weight loss and immune function, whereas the LPHC diet affected carbohydrate fermentation and may promote anti-oxidative function.</p>","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":" ","pages":"1-10"},"PeriodicalIF":6.4,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10614716/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49693748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathological changes of highly pathogenic <i>Bacillus cereus</i> on <i>Pelodiscus sinensis</i>.","authors":"Zidong Xiao, Mengmeng Cheng, Xiaowei Hu, Mingyang Xue, Nan Jiang, Wei Liu, Yuding Fan, Yan Meng, Chen Xu, Yong Zhou","doi":"10.1080/01652176.2023.2287191","DOIUrl":"10.1080/01652176.2023.2287191","url":null,"abstract":"<p><p>An outbreak of a disease with a high mortality rate occurred in a Chinese Softshell Turtle (<i>Pelodiscus sinensis</i>) farm in Hubei Province. This study isolated a highly pathogenic <i>Bacillus cereus</i> strain (Y271) from diseased <i>P. sinensis</i>. Y271 has <i>β</i> hemolysis, containing both Hemolysin BL (<i>hblA</i>, <i>hblC,</i> and <i>hblD</i>), Non-hemolytic enterotoxin, NHE (<i>nheA</i>, <i>nheB,</i> and <i>nheC</i>), and Enterotoxin FM (<i>entFM</i>) genes. Y271 is highly pathogenic against <i>P. sinensis</i> with an LD₅₀ = 6.80 × 10³ CFU/g weight. <i>B. cereus</i> was detected in multiple tissues of the infected <i>P. sinensis</i>. Among them, spleen tissue showed the highest copy number density (1.54 ± 0.12 × 10⁴ copies/mg). Multiple tissues and organs of diseased <i>P. sinensis</i> exhibited significant pathological damage, especially the spleen, liver, kidney, and intestine. It showed obvious tissue structure destruction, lesions, necrosis, red blood cells, and inflammatory cell infiltration. <i>B. cereus</i> proliferating in the spleen, liver, and other tissues was observed. The intestinal microbiota of the diseased <i>P. sinensis</i> was altered, with a greater abundance of Firmicutes, Fusobacterium, and Actinomyces than in the healthy group. <i>Allobaculum</i>, <i>Rothia</i>, <i>Aeromonas,</i> and <i>Clostridium</i> abundance were higher in the diseased group than in the healthy group. The number of unique microbial taxa (472) in the disease group was lower than that of the healthy group (705). Y271 was sensitive to multiple drugs, including florfenicol, enrofloxacin, neomycin, and doxycycline. <i>B. cereus</i> is the etiological agent responsible for the massive death of <i>P. sinensis</i> and reveals its potential risks during <i>P. sinensis</i> cultivation.</p>","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":" ","pages":"1-10"},"PeriodicalIF":6.4,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11003482/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138446989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Domestic and wild animal samples and diagnostic testing for SARS-CoV-2.","authors":"Megan R Miller,&nbsp;Elias Braun,&nbsp;Hon S Ip,&nbsp;Gregory H Tyson","doi":"10.1080/01652176.2023.2263864","DOIUrl":"10.1080/01652176.2023.2263864","url":null,"abstract":"<p><p>From the first cases in 2019, COVID-19 infections caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have resulted in over 6 million human deaths in a worldwide pandemic. SARS-CoV-2 is commonly spread from human to human through close contact and is capable of infecting both humans and animals. Worldwide, there have been over 675 animal outbreaks reported that resulted in over 2000 animal infections including domestic and wild animals. As the role of animal infections in the transmission, pathogenesis, and evolution of SARS-CoV-2 is still unfolding, accurate and reliable animal diagnostic tests are critical to aid in managing both human and animal health. This review highlights key animal samples and the three main diagnostic approaches used for animal testing: PCR, serology, and Next Generation Sequencing. Diagnostic results help inform (often difficult) clinical decision-making, but also possible ways to mitigate spread among pets, food supplies, or wildlife. A One Health approach has been key to monitoring the SARS-CoV-2 pandemic, as consistent human-animal interactions can lead to novel variants. Having multiple animal diagnostic tests for SARS-CoV-2 available is critical to ensure human, animal, and environmental health.</p>","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":" ","pages":"1-11"},"PeriodicalIF":6.4,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10614713/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41155701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of serum amyloid a within animal species focusing on the 1-25 amino acid region.","authors":"Natalie G Horgan,&nbsp;Kendall B E Moore,&nbsp;Jessica S Fortin","doi":"10.1080/01652176.2023.2267605","DOIUrl":"10.1080/01652176.2023.2267605","url":null,"abstract":"<p><p>AA amyloidosis, characterized by the misfolding of serum amyloid A (SAA) protein, is the most common amyloid protein disorder across multiple species. SAA is a positive-acute phase protein synthesized by the liver in response to inflammation or stress, and it normally associates with high-density lipoprotein at its N-terminus. In this study, we focused on the 1-25 amino acid (aa) region of the complete 104 aa SAA sequence to examine the aggregation propensity of AA amyloid. A library comprising eight peptides from different species was assembled for analysis. To access the aggregation propensity of each peptide region, a bioinformatic study was conducted using the algorithm TANGO. Congo red (CR) binding assays, Thioflavin T (ThT) assays, and transmission electron microscopy (TEM) were utilized to evaluate whether the synthesized peptides formed amyloid-like fibrils. All synthetic SAA 1-25 congeners resulted in amyloid-like fibrils formation (per CR and/or ThT staining and TEM detection) at the exception of the ferret SAA1-25 fragment, which generated plaque-like materials by TEM. Ten residues were preserved among SAA 1-25 congeners resulting in amyloid-like fibrils, i.e. F6, E9, A10, G13, D16, M17, A20, Y21, D23, and M24. Amino acid residues highlighted by this study may have a role in increasing the propensity for amyloid-like fibril formation. This study put an emphasis on region 1-25 in the mechanism of SAA1 misfolding.</p>","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":" ","pages":"1-8"},"PeriodicalIF":6.4,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10614707/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41173917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiple roles of LncRNA-BMNCR on cell proliferation and apoptosis by targeting miR-145/CBFB axis in BMECs.","authors":"Yujia Sun,&nbsp;Tianqi Zhao,&nbsp;Yaoyao Ma,&nbsp;Xinyi Wu,&nbsp;Shuangfeng Chu,&nbsp;Zhangping Yang","doi":"10.1080/01652176.2023.2262525","DOIUrl":"10.1080/01652176.2023.2262525","url":null,"abstract":"<p><p>Bovine mastitis is one of the most serious and costly disease affecting dairy cattle production. The present study explored the inflammatory response and autoprotective mechanism of a novel specific high expression BMNCR (bovine mastitis related long non-coding RNA) in <i>S. aureus</i> induced mastitis by miR-145/CBFB axis in dairy cows from the perspective of molecular genetics. In bovine mammary epithelial cells, we preformed loss of function experiments to detect changes in cytokine, proliferation and apoptosis by qRT-PCR, western blot, flow cytometry and EdU staining. The results demonstrated that BMNCR significantly increased cell apoptosis, and inhibited cell proliferation. However, the secretion of IL-1α, IL-2, IL-6, IL-8 and IL-12 were enhanced after knock-down BMNCR. Bioinformatics analysis demonstrated that BMNCR could target 8 miRNAs, in-depth analyses indicated that BMNCR acts as a molecular sponge for bta-miR-145 and CBFB was one of 23 target gene of bta-miR-145 . The results of the present study demonstrated that the role of BMNCR in <i>S. aureus</i> induced mastitis can be mediated by sponge bta-miR-145 activating CBFB expression. BMNCR could be a potential target for mastitis diagnosis and therapy, which may enrich the theoretical research of therapeutic intervention, and further increase milk yield and improve milk quality.</p>","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":" ","pages":"1-11"},"PeriodicalIF":6.4,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/16/99/TVEQ_43_2262525.PMC10566406.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41177446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}