Veterinary Quarterly最新文献_第10页

Canine parvovirus in North-East India: a phylogenetic and evolutionary analysis. 印度东北部的犬细小病毒：系统发育和进化分析。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-10-01 DOI: 10.1080/01652176.2024.2408742

Kiran Jayappa, Tridib Kumar Rajkhowa, Satish S Gaikwad

{"title":"Canine parvovirus in North-East India: a phylogenetic and evolutionary analysis.","authors":"Kiran Jayappa, Tridib Kumar Rajkhowa, Satish S Gaikwad","doi":"10.1080/01652176.2024.2408742","DOIUrl":"10.1080/01652176.2024.2408742","url":null,"abstract":"Canine parvovirus type 2 (CPV-2) infection in dogs is considered as one of the most common cause of morbidity and mortality in young dogs and continues to occur with high incidence worldwide. Despite a single-stranded DNA virus, CPV-2 possesses a high mutation rate which has led to the development of new variants from time to time. These variants are classically classified based on the amino acid markers present in the VP2 gene. In this study, we examined 20 different cases of CPV-2 infection from seven different states of the North East region (NER) of India. The near-complete genome sequences of all these isolates were subjected to phylodynamic and phylogeographic analysis to evaluate the genetic diversity and geographical spread of CPV-2 variants. Analysis of the deduced amino acid sequences revealed residues characteristic of the 'Asian CPV-2c lineage' in all the 20 sequences confirming it as the dominant strain circulating in NER, India. The phylogenetic analysis based on the whole genome showed that all 20 sequences formed a monophyletic clade together with other Asian CPV-2c sequences. Furthermore, phylogeographic analysis based on the VP2 gene showed the likely introduction of Asian CPV-2c strain to India from China. This study marks the first comprehensive report elucidating the molecular epidemiology of CPV-2 in India.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-13"},"PeriodicalIF":7.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11445921/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142331965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of the seroprevalence to equine coronavirus and SARS-CoV-2 in healthy adult horses recently imported to the United States. 近期进口美国的健康成年马对马冠状病毒和SARS-CoV-2的血清阳性率调查

IF 6.4 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-03-18 DOI: 10.1080/01652176.2023.2288876

Nicola Pusterla, Kaila Lawton, Samantha Barnum

{"title":"Investigation of the seroprevalence to equine coronavirus and SARS-CoV-2 in healthy adult horses recently imported to the United States.","authors":"Nicola Pusterla, Kaila Lawton, Samantha Barnum","doi":"10.1080/01652176.2023.2288876","DOIUrl":"10.1080/01652176.2023.2288876","url":null,"abstract":"Adult horses are susceptible to equine coronavirus (ECoV) and severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), although, only ECoV has been linked to clinical disease. Little information is available regarding the seroprevalence against ECoV and SARS-CoV-2 in adult healthy horses. The goal of the present study was to determine the seroprevalence against two coronaviruses known to infect horses using convenience samples collected from horses recently imported from Europe to the United States from 2019 to 2023. A total of 385 banked serum samples were tested against ECoV and SARS-CoV-2 using previously validated ELISA assays. Prevalence factors including date of arrival in the United States, signalment and country of origin were available for the majority of the horses. A total of 9/385 (2.3%) and 4/385 (1.0%) horses tested seropositive for ECoV and SARS-CoV-2, respectively. The ECoV seropositive horses were all mares, ages 4 to 26 years (median 9 years) and originated from Germany, the Netherlands, Ireland, Belgium and Italy. These mares were predominantly imported during the summer and fall months. All SARS-CoV-2 seropositive horses were mares ages 5 to 10 years (median 7.5 years) imported from the Netherlands and the United Kingdom. The majority of the SARS-CoV-2 seropositive horses were imported during the colder months of the year. The study results support the presence of ECoV in Europe and report on the first SARS-CoV-2 seropositive healthy adult horses outside the United States. Commingling for movements by air and close contact to humans may predispose transmission with ECoV and SARS-CoV-2, respectively.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":" ","pages":"1-6"},"PeriodicalIF":6.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10949836/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138446988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular vesicles secreted by equine adipose mesenchymal stem cells preconditioned with transforming growth factor β-1 are enriched in anti-fibrotic miRNAs and inhibit the expression of fibrotic genes in an in vitro system of endometrial stromal cells fibrosis. 经转化生长因子β-1预处理的马脂肪间充质干细胞分泌的胞外囊泡富含抗纤维化的miRNA，并能抑制子宫内膜基质细胞纤维化体外系统中纤维化基因的表达。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-07-31 DOI: 10.1080/01652176.2024.2384906

Yat Sen Wong, Ana Carolina Mançanares, Felipe Navarrete, Pamela Poblete, Lídice Mendez-Pérez, Joel Cabezas, Gonzalo Riadi, Lleretny Rodríguez-Alvarez, Fidel Ovidio Castro

{"title":"Extracellular vesicles secreted by equine adipose mesenchymal stem cells preconditioned with transforming growth factor β-1 are enriched in anti-fibrotic miRNAs and inhibit the expression of fibrotic genes in an in vitro system of endometrial stromal cells fibrosis.","authors":"Yat Sen Wong, Ana Carolina Mançanares, Felipe Navarrete, Pamela Poblete, Lídice Mendez-Pérez, Joel Cabezas, Gonzalo Riadi, Lleretny Rodríguez-Alvarez, Fidel Ovidio Castro","doi":"10.1080/01652176.2024.2384906","DOIUrl":"10.1080/01652176.2024.2384906","url":null,"abstract":"Mare endometrosis is a major reproductive problem associated with low fertility and is characterized by persistent inflammation, TGFβ-1 signaling, and consequently, extracellular matrix deposition, which compromises endometrial glands. Mesenchymal stem cell-based products (MSCs), such as extracellular vesicles (EVs), have gained attention due to the regulatory effects exerted by their miRNA cargo. Here, we evaluated the impact of preconditioning equine adipose mesenchymal stem cells with TGFβ-1 for short or long periods on the anti-fibrotic properties of secreted extracellular vesicles. MSCs were isolated from six healthy horses and exposed to TGFβ-1 for 4, 24, and 0 h. The expression of anti-fibrotic and pro-fibrotic miRNAs and mRNAs in treated cells and miRNAs in the cargo of secreted extracellular vesicles was measured. The resulting EVs were added for 48 h to endometrial stromal cells previously induced to a fibrotic status. The expression of anti-fibrotic and pro-fibrotic genes and miRNAs was evaluated in said cells using qPCR and next-generation sequencing. Preconditioning MSCs with TGFβ-1 for 4 h enriched the anti-fibrotic miRNAs (mir29c, mir145, and mir200) in cells and EVs. Conversely, preconditioning the cells for 24 h leads to a pro-fibrotic phenotype overexpressing mir192 and mir433. This finding might have implications for developing an EV-based protocol to treat endometrial fibrosis in mares.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-11"},"PeriodicalIF":7.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11295685/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141861601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection and quantification of Brucella abortus DNA in water buffaloes (bubalus bubalis) using droplet digital polymerase chain reaction. 利用液滴数字聚合酶链式反应检测和量化水牛体内流产布鲁氏菌的DNA

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-08-15 DOI: 10.1080/01652176.2024.2390944

Giovanna Fusco, Lorena Cardillo, Ornella Valvini, Alessia Pucciarelli, Gerardo Picazio, Anna Cerrone, Michele Napoletano, Roberta Pellicanò, Maria Ottaiano, Claudio de Martinis, Francesca De Falco, Anna Cutarelli, Emanuela Sannino, Giorgia Borriello, Manuela Tittarelli, Sante Roperto, Esterina De Carlo

{"title":"Detection and quantification of Brucella abortus DNA in water buffaloes (bubalus bubalis) using droplet digital polymerase chain reaction.","authors":"Giovanna Fusco, Lorena Cardillo, Ornella Valvini, Alessia Pucciarelli, Gerardo Picazio, Anna Cerrone, Michele Napoletano, Roberta Pellicanò, Maria Ottaiano, Claudio de Martinis, Francesca De Falco, Anna Cutarelli, Emanuela Sannino, Giorgia Borriello, Manuela Tittarelli, Sante Roperto, Esterina De Carlo","doi":"10.1080/01652176.2024.2390944","DOIUrl":"10.1080/01652176.2024.2390944","url":null,"abstract":"Brucellosis represents a major public health concern worldwide. Human transmission is mainly due to the consumption of unpasteurized milk and dairy products of infected animals. The gold standard for the diagnosis of Brucella spp in ruminants is the bacterial isolation, but it is time-consuming. Polymerase Chain Reaction (PCR) is a quicker and more sensitive technique than bacterial culture. Droplet digital PCR (ddPCR) is a novel molecular assay showing high sensitivity in samples with low amount of DNA and lower susceptibility to amplification inhibitors. Present study aimed to develop a ddPCR protocol for the detection of Brucella abortus in buffalo tissue samples. The protocol was validated using proficiency test samples for Brucella spp by real time qPCR. Furthermore, 599 tissue samples were examined. Among reference materials, qPCR and ddPCR demonstrated same performance and were able to detect up to 225 CFU/mL. Among field samples, ddPCR showed higher sensitivity (100%), specificity and accuracy of 93.4% and 94.15%, respectively. ddPCR could be considered a promising technique to detect B. abortus in veterinary specimens, frequently characterized by low amount of bacteria, high diversity in matrices and species and poor storage conditions.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-8"},"PeriodicalIF":7.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11328813/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141989496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacokinetics, optimal dosages and withdrawal time of amoxicillin in Nile tilapia (Oreochromis niloticus) reared at 25 and 30 °C. 尼罗罗非鱼（Oreochromis niloticus）在 25 和 30 摄氏度条件下饲养时阿莫西林的药代动力学、最佳剂量和停药时间。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-08-27 DOI: 10.1080/01652176.2024.2396573

Tirawat Rairat, Yi-Ping Lu, Wan-Cih Ho, Hual-Jhong Ke, Chi-Chung Chou

{"title":"Pharmacokinetics, optimal dosages and withdrawal time of amoxicillin in Nile tilapia (Oreochromis niloticus) reared at 25 and 30 °C.","authors":"Tirawat Rairat, Yi-Ping Lu, Wan-Cih Ho, Hual-Jhong Ke, Chi-Chung Chou","doi":"10.1080/01652176.2024.2396573","DOIUrl":"10.1080/01652176.2024.2396573","url":null,"abstract":"Knowledge of amoxicillin (AMX) pharmacokinetics (PK) and tissue residues in fish, which is necessary for prudent drug use, remains limited. The study aimed to explore the PK characteristics of AMX in Nile tilapia (Oreochromis niloticus) reared at 25 and 30 °C as well as to determine optimal dosages and drug withdrawal time (WDT). In the PK investigation, the fish received a single dose of 40 mg/kg AMX via oral gavage, and the optimal dosage was determined by the pharmacokinetic-pharmacodynamic approach. In the tissue residue study, the fish were orally gavaged with 40 mg/kg/day AMX once daily for 5 days and the WDT was established by the linear regression analysis. The results revealed the temperature-dependent drug elimination; the clearance relative to bioavailability (CL/F) and elimination half-life at 30 °C (0.180 L/kg/h and 6.06 h, respectively) were about twice those at 25 °C (0.090 L/kg/h and 10.49 h, respectively). The optimal dosages at the minimum inhibitory concentration (MIC) of 2 μg/mL were 10.97 (25 °C) and 41.03 (30 °C) mg/kg/day, respectively. Finally, following the multiple oral administration, the muscle/skin residue of AMX on day 1 after the last dosing at 25 and 30 °C were 548 and 264 ng/g, respectively. The average tissue residues were depleted below the maximum residue limits (MRL) of 50 μg/kg on day 5 (25 °C) and 3 (30 °C), respectively, and the WDT were 6 and 4 days when rearing at 25 and 30 °C, respectively. This knowledge serves as a practical guideline for responsible use of AMX in treating bacterial diseases in Nile tilapia aquaculture.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-9"},"PeriodicalIF":7.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11360631/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142082524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural and sequence analysis of the RPO30 gene of sheeppox and goatpox viruses from India. 印度羊痘和山羊痘病毒 RPO30 基因的结构和序列分析。

IF 6.4 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-03-25 DOI: 10.1080/01652176.2024.2331524

Gundallahalli Bayyappa Manjunatha Reddy, Krishnappa Sumana, Revanaiah Yogisharadhya, Hosakote Venkatappa Mohan, Vijaykumar Kolar Lavanya, Basavarajappa Harlipura Chethankumar, Nayakwadi Shivasharanappa, Mani Saminathan, Sajjanar Basavaraj, Kuldeep Dhama, Shivachandra Bhadravati Sathish

{"title":"Structural and sequence analysis of the RPO30 gene of sheeppox and goatpox viruses from India.","authors":"Gundallahalli Bayyappa Manjunatha Reddy, Krishnappa Sumana, Revanaiah Yogisharadhya, Hosakote Venkatappa Mohan, Vijaykumar Kolar Lavanya, Basavarajappa Harlipura Chethankumar, Nayakwadi Shivasharanappa, Mani Saminathan, Sajjanar Basavaraj, Kuldeep Dhama, Shivachandra Bhadravati Sathish","doi":"10.1080/01652176.2024.2331524","DOIUrl":"10.1080/01652176.2024.2331524","url":null,"abstract":"Sheeppox and goatpox are transboundary viral diseases of sheep and goats that cause significant economic losses to small and marginal farmers worldwide, including India. Members of the genus Capripoxvirus (CaPV), namely Sheeppox virus (SPPV), Goatpox virus (GTPV), and Lumpy skin disease virus (LSDV), are antigenically similar, and species differentiation can only be accomplished using molecular approaches. The present study aimed to understand the molecular epidemiology and host specificity of SPPV and GTPV circulating in India through sequencing and structural analysis of the RNA polymerase subunit-30 kDa (RPO30) gene. A total of 29 field isolates from sheep (n = 19) and goats (n = 10) belonging to different geographical regions of India during the period: Year 2015 to 2023, were analyzed based on the sequence and structure of the full-length RPO30 gene/protein. Phylogenetically, all the CaPV isolates were separated into three major clusters: SPPV, GTPV, and LSDV. Multiple sequence alignment revealed a highly conserved RPO30 gene, with a stretch of 21 nucleotide deletion in all SPPV isolates. Additionally, the RPO30 gene of the Indian SPPV and GTPV isolates possessed several species-specific conserved signature residues/motifs that could act as genotyping markers. Secondary structure analysis of the RPO30 protein showed four α-helices, two loops, and three turns, similar to that of the E4L protein of vaccinia virus (VACV). All the isolates in the present study exhibited host preferences across different states of India. Therefore, in order to protect vulnerable small ruminants from poxviral infections, it is recommended to take into consideration a homologous vaccination strategy.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-12"},"PeriodicalIF":6.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10964832/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140208178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Predictive value of TP53 RNAscope®in situ hybridization and p53 immunohistochemistry for TP53 mutational status in canine diffuse large B-cell lymphoma. TP53 RNAscope® 原位杂交和 p53 免疫组织化学对犬弥漫大 B 细胞淋巴瘤 TP53 突变状态的预测价值。

IF 6.4 2区农林科学

Veterinary Quarterly Pub Date : 2024-09-16 DOI: 10.1080/01652176.2024.2403453

Greta Foiani,Luca Licenziato,Laura Marconato,Antonella Fanelli,Erica Melchiotti,Claudia Zanardello,Luca Aresu,Marta Vascellari

{"title":"Predictive value of TP53 RNAscope®in situ hybridization and p53 immunohistochemistry for TP53 mutational status in canine diffuse large B-cell lymphoma.","authors":"Greta Foiani,Luca Licenziato,Laura Marconato,Antonella Fanelli,Erica Melchiotti,Claudia Zanardello,Luca Aresu,Marta Vascellari","doi":"10.1080/01652176.2024.2403453","DOIUrl":"https://doi.org/10.1080/01652176.2024.2403453","url":null,"abstract":"TP53 mutations are associated with short survival and poor treatment response in canine diffuse large B-cell lymphoma (cDLBCL). The expression of TP53 by RNAscope® in situ hybridization and p53 by immunohistochemistry (IHC) was investigated in 37 formalin-fixed paraffin-embedded cDLBCL, to assess their correlation with TP53 mutational status and to evaluate their prognostic value. TP53 was detected in all samples by RNAscope®. Ten of 37 (27%) cases expressed p53 by IHC, with highly variable percentage of positive cells. TP53 RNAscope® scores and p53 IHC results were not correlated. The expression of TP53 by RNAscope® was not influenced by its mutational status. Conversely, p53 IHC and TP53 mutations were significantly associated. p53 IHC predicted TP53 genetic mutations with high accuracy (97.3%). All TP53-mutated samples carrying missense mutations exhibited p53 expression by IHC, while all wild-type cases and a single case with frameshift insertion were negative. In univariable analysis, p53 IHC was associated with shorter time to progression (TTP) and lymphoma-specific survival (LSS). Nevertheless, in multivariable analysis, only treatment significantly affected TTP and LSS. These findings suggest p53 IHC is an accurate, cost-effective tool for predicting TP53 mutations in cDLBCL, unlike TP53 RNAscope®, though its prognostic value requires further validation.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"41 1","pages":"1-9"},"PeriodicalIF":6.4,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142251241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the feasibility of equine in-vivo ultrasound technique for the medial branch of the dorsal ramus of the cervical spinal nerves 评估颈脊神经背侧支内侧支马体内超声技术的可行性

IF 6.4 2区农林科学

Veterinary Quarterly Pub Date : 2024-09-13 DOI: 10.1080/01652176.2024.2403456

Irene Nocera, Chiara Di Franco, Benedetta Sorvillo, Benedetta Aliboni, Elena Bucchioni, Micaela Sgorbini, Giulia Sala, Simonetta Citi

引用次数: 0

Modulating effects of heat-killed and live Limosilactobacillus reuteri PSC102 on the immune response and gut microbiota of cyclophosphamide-treated rats 热杀死和活的Limosilactobacillus reuteri PSC102对环磷酰胺治疗大鼠的免疫反应和肠道微生物群的调节作用

IF 6.4 2区农林科学

Veterinary Quarterly Pub Date : 2024-04-29 DOI: 10.1080/01652176.2024.2344765

Md. Sekendar Ali, Eon-Bee Lee, Yixian Quah, Syed Al Jawad Sayem, Muhammad Aleem Abbas, Kyoungho Suk, Seung-Jin Lee, Seung-Chun Park

引用次数: 0

The forecasting power of the mucin-microbiome interplay in livestock respiratory diseases 牲畜呼吸道疾病中粘蛋白-微生物组相互作用的预测能力