印度羊痘和山羊痘病毒 RPO30 基因的结构和序列分析。

IF 7.9 2区 农林科学 Q1 VETERINARY SCIENCES
Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-03-25 DOI:10.1080/01652176.2024.2331524
Gundallahalli Bayyappa Manjunatha Reddy, Krishnappa Sumana, Revanaiah Yogisharadhya, Hosakote Venkatappa Mohan, Vijaykumar Kolar Lavanya, Basavarajappa Harlipura Chethankumar, Nayakwadi Shivasharanappa, Mani Saminathan, Sajjanar Basavaraj, Kuldeep Dhama, Shivachandra Bhadravati Sathish
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引用次数: 0

摘要

绵羊痘(Sheeppox)和山羊痘(Goatpox)是绵羊和山羊的跨境病毒性疾病,给包括印度在内的全世界小型和边缘化养殖户造成了重大经济损失。绵羊痘病毒属(Capripoxvirus,CaPV)的成员,即绵羊痘病毒(Sheeppox virus,SPPV)、山羊痘病毒(Goatpox virus,GTPV)和结节性皮肤病病毒(Lumpy skin disease virus,LSDV),在抗原上非常相似,只有通过分子方法才能进行物种区分。本研究旨在通过对 RNA 聚合酶亚基-30 kDa(RPO30)基因进行测序和结构分析,了解印度流行的 SPPV 和 GTPV 的分子流行病学和宿主特异性。在 2015 年至 2023 年期间,共从印度不同地理区域的绵羊(n = 19)和山羊(n = 10)中分离出 29 个野外样本:根据全长 RPO30 基因/蛋白质的序列和结构进行了分析。从系统发生学角度看,所有 CaPV 分离物被分为三大类:SPPV、GTPV 和 LSDV。多重序列比对显示,所有 SPPV 分离物的 RPO30 基因高度保守,有一段 21 个核苷酸的缺失。此外,印度 SPPV 和 GTPV 分离物的 RPO30 基因具有几个物种特异性保守残基/基序,可作为基因分型标记。RPO30 蛋白的二级结构分析表明有四个 α-螺旋、两个环和三个转折,与疫苗病毒(VACV)的 E4L 蛋白相似。本研究中的所有分离物都表现出对印度不同邦寄主的偏好。因此,为了保护易感小型反刍动物免受痘病毒感染,建议考虑同源疫苗接种策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Structural and sequence analysis of the RPO30 gene of sheeppox and goatpox viruses from India.

Sheeppox and goatpox are transboundary viral diseases of sheep and goats that cause significant economic losses to small and marginal farmers worldwide, including India. Members of the genus Capripoxvirus (CaPV), namely Sheeppox virus (SPPV), Goatpox virus (GTPV), and Lumpy skin disease virus (LSDV), are antigenically similar, and species differentiation can only be accomplished using molecular approaches. The present study aimed to understand the molecular epidemiology and host specificity of SPPV and GTPV circulating in India through sequencing and structural analysis of the RNA polymerase subunit-30 kDa (RPO30) gene. A total of 29 field isolates from sheep (n = 19) and goats (n = 10) belonging to different geographical regions of India during the period: Year 2015 to 2023, were analyzed based on the sequence and structure of the full-length RPO30 gene/protein. Phylogenetically, all the CaPV isolates were separated into three major clusters: SPPV, GTPV, and LSDV. Multiple sequence alignment revealed a highly conserved RPO30 gene, with a stretch of 21 nucleotide deletion in all SPPV isolates. Additionally, the RPO30 gene of the Indian SPPV and GTPV isolates possessed several species-specific conserved signature residues/motifs that could act as genotyping markers. Secondary structure analysis of the RPO30 protein showed four α-helices, two loops, and three turns, similar to that of the E4L protein of vaccinia virus (VACV). All the isolates in the present study exhibited host preferences across different states of India. Therefore, in order to protect vulnerable small ruminants from poxviral infections, it is recommended to take into consideration a homologous vaccination strategy.

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来源期刊
Veterinary Quarterly
Veterinary Quarterly VETERINARY SCIENCES-
CiteScore
13.10
自引率
1.60%
发文量
18
审稿时长
>24 weeks
期刊介绍: Veterinary Quarterly is an international open access journal which publishes high quality review articles and original research in the field of veterinary science and animal diseases. The journal publishes research on a range of different animal species and topics including: - Economically important species such as domesticated and non-domesticated farm animals, including avian and poultry diseases; - Companion animals (dogs, cats, horses, pocket pets and exotics); - Wildlife species; - Infectious diseases; - Diagnosis; - Treatment including pharmacology and vaccination
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