Canadian Journal of Infectious Diseases & Medical Microbiology最新文献

{"title":"Alterations of the Intestinal Mucosal Barrier and Gut Fungal Microbiome in Asymptomatic HIV-Infected Patients.","authors":"Wenjie Li, Yong Qing, Qiuyue Yu, Hulian Zhang, Zhen Rang, Shuangli Li, Fan Cui","doi":"10.1155/cjid/6995192","DOIUrl":"10.1155/cjid/6995192","url":null,"abstract":"<p><p>Damage to the intestinal mucosal barrier and dysbiosis of the gut microbiota are critical factors in HIV progression, reciprocally influencing each other. Besides bacteria, the fungal microbiota, a significant component of the gut, plays a pivotal role in this dysregulation. This study aims to investigate changes in the gut mucosal barrier and mycobiota during the initial stages of HIV infection, focusing on the involvement of intestinal fungi and their secretions in mucosal damage. Peripheral blood, intestinal mucosa, and fecal samples were collected from 13 asymptomatic HIV-infected individuals at the non-AIDS stage and 13 healthy controls. Assessments included colonoscopy, immune function analysis, and measurement of mucosal damage markers (LPS, I-FABP, and D-LA) and inflammatory cytokines (IL-6 and IL-18). Additionally, Claudin-1 levels in mucosal samples and fungal profiles in fecal samples were evaluated. The study found that colonic abnormalities were significantly more prevalent in the HIV group compared to healthy controls (<i>p</i> < 0.001) and Claudin-1 levels were notably lower in the HIV group (<i>p</i> <  0.001). <i>Candida albicans</i> (<i>p</i>=0.0084), its secretion SAP1 (<i>p</i>=0.023), and the levels of IL-18 (<i>p</i>=0.0016) and IL-6 (<i>p</i> < 0.001) were all significantly higher in the HIV group. CD4+ T-cell counts were positively correlated with Claudin-1 expression (<i>p</i>=0.034, <i>r</i> = 0.417). <i>Candida albicans</i> showed negative correlations with several virulence factors, while other fungi exhibited varied correlations. Additionally, Claudin-1 levels were significantly negatively correlated with <i>Candida albicans</i> (<i>p</i>=0.013, <i>r</i> = -0.668), SAP1 (<i>p</i>=0.027, <i>r</i> = -0.609), IL-18 (<i>p</i> < 0.001, <i>r</i> = -0.922), and IL-6 (<i>p</i> < 0.001, <i>r</i> = -0.920). Overall, these findings suggest that asymptomatic HIV-infected individuals have already exhibited intestinal mucosal damage in the early stage and highlight the critical role of <i>Candida albicans</i> and its secretions in early-stage intestinal mucosal barrier damage.</p>","PeriodicalId":50715,"journal":{"name":"Canadian Journal of Infectious Diseases & Medical Microbiology","volume":"2024 ","pages":"6995192"},"PeriodicalIF":2.6,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11779998/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143068725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Features of Long COVID Patients Coinfected With <i>Mycoplasma pneumoniae</i>.","authors":"Xiaodan Zhu, Yanhua Li, Jinghua Wang, Weifei Gao","doi":"10.1155/cjid/7213129","DOIUrl":"10.1155/cjid/7213129","url":null,"abstract":"<p><p><b>Background:</b> Since the SARS-CoV-2 pandemic, many patients have suffered prolonged complications, called \"long COVID.\" <i>Mycoplasma pneumoniae</i> is a common respiratory pathogen. Reports of simultaneous long COVID and <i>M. pneumoniae</i> infections are rare in the literature. <b>Methods:</b> We analyzed the clinical data of patients with long COVID-19 who visited the Respiratory Clinic of The Affiliated Hospital of Hangzhou Normal University between January 1 and January 31, 2023, together with their laboratory and radiographic findings, with Pearson's <i>χ</i> ² test. <b>Results:</b> Fifty-two patients diagnosed with both long COVID and <i>M. pneumoniae</i> infection and 77 with long COVID only were compared. The ages, clinical symptoms, and comorbidities of the two groups did not differ significantly (<i>p</i> > 0.05). However, sex and imaging findings differed between the groups. <b>Conclusions:</b> Our study showed that long COVID-<i>M. pneumoniae</i> coinfection was more commonly seen in females and patients with typical chest computed tomography (CT) images.</p>","PeriodicalId":50715,"journal":{"name":"Canadian Journal of Infectious Diseases & Medical Microbiology","volume":"2024 ","pages":"7213129"},"PeriodicalIF":2.6,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11646145/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142830703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retrospective Analysis of Coronavirus SARS-CoV-2 Antibody Levels in COVID-19 Convalescent Plasma From Blood Donors.","authors":"Ji He, Yanjun Zhang, Jie Dong, Wei Zhang, Faming Zhu","doi":"10.1155/cjid/4366502","DOIUrl":"10.1155/cjid/4366502","url":null,"abstract":"<p><p><b>Objective:</b> To detect and analyze coronavirus SARS-CoV-2 antibody levels in convalescent plasma from donors who have recovered from COVID-19. <b>Methods:</b> Plasma samples from 88 donors aged 20-54 years who were diagnosed with COVID-19 and who were eligible to donate from Zhejiang Province, China, were collected as the experimental group, and 56 samples from healthy blood donors were used as controls. Anti-SARS-CoV-2 antibodies, including Ab and IgM, were detected via chemiluminescent immunoassay, and neutralizing antibodies were measured via the microneutralization method. <b>Results:</b> The positive rates of total and IgM antibodies against SARS-CoV-2 were 97.7% (86/88) and 52.3% (46/88), respectively, in the plasma samples of 88 patients who recovered from COVID-19. After 160 and 320 dilutions of the total antibody-positive samples, the positive rates were 61.6% (53/86) and 39.5% (34/86), respectively. The titer of neutralizing antibodies was 16-256 in 53 SARS-CoV-2-positive samples after 160-fold dilution of total antibodies. The titer of neutralizing antibody was 48-256 in 34 samples that were still positive after 320-fold dilution of total antibody. Among the 88 samples, 86 had titers > 4, and 10 had high titers > 80. In 2 patients with neutralizing antibody titers < 4, SARS-CoV-2 total antibody and IgM antibodies were negative. The correlation coefficient between total antibody strength and neutralizing antibody titer was 0.5198 (high correlation). The total antibody and IgM antibodies of 56 healthy blood donors were negative. <b>Conclusions:</b> There are individual differences in plasma antibody titers among convalescent patients. Antibody detection is helpful for screening out plasma with high antibody titers for the treatment of patients with severe disease.</p>","PeriodicalId":50715,"journal":{"name":"Canadian Journal of Infectious Diseases & Medical Microbiology","volume":"2024 ","pages":"4366502"},"PeriodicalIF":2.6,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11637619/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142820087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mucosal Hub Bacteria as Potential Targets for Improving High-Fat Diet-Related Intestinal Barrier Injury.","authors":"Li Shao, Binbin Zhang, Yu Song, Zhe Lyu, Weishi Zhang, Wenjun Yang, Jinlong Fu, Jie Li, Junping Shi","doi":"10.1155/cjid/3652740","DOIUrl":"10.1155/cjid/3652740","url":null,"abstract":"<p><p><b>Background:</b> Intestinal barrier injury contributes to multiple diseases such as obesity and diabetes, whereas no treatment options are available. <b>Methods:</b> Due to close interactions between mucosal microbiome and intestinal barrier, we evaluated the potential of mucosal bacteria in providing targets for high-fat diet (HFD)-related intestinal barrier injury. Whole-genome metagenomics was used to evaluate mucosal microbiome, while intestinal barrier injury was estimated using serum LPS, FITC-dextran intensity, and ZO-1 protein. <b>Results:</b> We found that HFD induced significant fat accumulation in epididymal tissue at weeks 4 and 12, while ALT, LDL, and TC increased at week 12. Intestinal barrier injury was confirmed by elevated serum LPS at both weeks, upregulated FITC-dextran intensity, and decreased ZO-1 protein at week 12. Fourteen species such as <i>Phocaeicola vulgatus</i> differed in HFD-fed mice. The co-occurrence network of mucosal microbiome shifted from scale-free graph in controls to nearly random graph in HFD-fed mice. Besides, 10 hub bacteria especially <i>Bacteroides ovatus</i> decreased drastically in both mucosal and fecal samples of HFD-fed mice, correlated with intestinal permeability, ALT, and KEGG pathways such as \"Mitochondrial biogenesis\" and \"metabolism\". Moreover, <i>Bacteroides ovatus</i> has been confirmed to improve intestinal barrier function in a recent study. <b>Conclusion:</b> Mucosal hub bacteria can provide potential targets for improving HFD-related intestinal barrier function.</p>","PeriodicalId":50715,"journal":{"name":"Canadian Journal of Infectious Diseases & Medical Microbiology","volume":"2024 ","pages":"3652740"},"PeriodicalIF":2.6,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11617042/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142781367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological Characterization and Genotyping of <i>Acanthamoeba</i> Isolates From Oral and Nasal Samples of Cancer Patients in Kashan, Iran.","authors":"Sima Rasti, Tayebeh Taghipour, Mahdi Delavari, Hossein Hooshyar, Gholam Abbas Moosavi, Mohsen Arbabi","doi":"10.1155/2024/4071707","DOIUrl":"10.1155/2024/4071707","url":null,"abstract":"<p><p><b>Background:</b> <i>Acanthamoeba</i> species are recognized as the most prevalent free-living amoebae (FLA). They can cause granulomatous amebic encephalitis (GAE) and pulmonary and ocular infections. The present study aimed to isolate and identify <i>Acanthamoeba</i> genotypes in cancer patients referred to Kashan's hospitals in Central Iran. <b>Methods:</b> This cross-sectional study was conducted with oral and nasal swab samples collected from a hundred cancer patients referred to Kashan's Beheshti and Yasrebi hospitals during 2019-2020. The samples were cultured in 1.5% non-nutrient agar (NNA) with heat-killed <i>Escherichia coli</i> and examined for \"FLA.\" A polymerase chain reaction (PCR) assay amplifying the 18S rRNA gene was performed, and <i>Acanthamoeba</i>-positive isolates were subjected to nucleotide sequencing to identify their genotypes. <b>Results:</b> The prevalence of <i>Acanthamoeba</i> infection was 51% in the oral cavity and 38% in the nasal samples of cancer patients. The most frequent <i>Acanthamoeba</i> cysts were (51.3%) wrinkled polygonal and sized 9.55-11.5 μm (Group II). <i>Acanthamoeba</i> genotypes T4, T11, and T5 were identified in the oral cavity samples, whereas T4 and T11 were detected in the nasal samples. <b>Conclusion:</b> The prevalence of <i>Acanthamoeba</i> infection in oral and nasal cancer patients was higher in Kashan, Iran, compared to other countries. Due to the high rate of oral <i>Acanthamoeba</i> contamination, oral sampling is recommended for better detection of this protozoan. Since T4 is the predominant genotype that can cause serious diseases in high-risk groups, increasing physicians' awareness of infections associated with <i>Acanthamoeba</i> and preventive and control measures are strongly suggested.</p>","PeriodicalId":50715,"journal":{"name":"Canadian Journal of Infectious Diseases & Medical Microbiology","volume":"2024 ","pages":"4071707"},"PeriodicalIF":2.6,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11578656/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142683167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of Varicella Zoster Virus (VZV) in Central Nervous System Infectious Syndromes.","authors":"Viktoria Yakovenko, Pnina Ciobotaro, Rita Bardenstein, Margalit Zusev, Oren Zimhony","doi":"10.1155/cjid/6664417","DOIUrl":"https://doi.org/10.1155/cjid/6664417","url":null,"abstract":"<p><p><b>Background:</b> We describe the proportion of VZV infection in central nervous system (CNS) infectious syndromes in a single Israeli medical center. <b>Methods:</b> An observational cohort study was conducted in Kaplan Medical Center (a secondary hospital, Israel) between July 1, 2014, and March 31, 2019. Included were adult patients (≥ 16 years old) with CNS infection with an aseptic CSF profile that were subjected to molecular tests for herpes viruses, HSV either 1 or 2, VZV, enteroviruses, and IgM for West Nile virus (WNV). <b>Results:</b> Clinical presentation suggestive of CNS infection led to lumbar puncture and CSF analysis in 1500 patients yielding 178 cases with aseptic CSF profile. For 62/109 (55.9%) cases of meningitis, the etiology remained unknown, enterovirus accounted for 21 cases (18.9%), VZV for 16 (14.4%), and HSV 1 for 7 (6.3%). One case each of bacterial, fungal, and parainfluenza virus accounted for the other 3 cases. For 30/63 (47.6%) cases of encephalitis, the etiology remained unknown, HSV accounted for 11 cases (17.5%), VZV for 8 (12.7%), and WNV for 10 (15.9%); in two cases, enterovirus was identified and one case of influenza A and one of mycoplasma, accounted for the rest. In six patients with myelitis VZV was identified in 4 (66.7%). Notably, a typical herpetic rash was identified in only 11/28 (39.28%) of cases of VZV CNS infection. <b>Conclusions:</b> VZV is a significant cause of viral CNS infections. In the majority of patients with neurologic syndrome and evident VZV there is no association with a typical herpetic rash. These results apply to Israeli population and likely to other populations with similar background of VZV past infections.</p>","PeriodicalId":50715,"journal":{"name":"Canadian Journal of Infectious Diseases & Medical Microbiology","volume":"2024 ","pages":"6664417"},"PeriodicalIF":2.6,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11611393/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142774564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does Fever Caused by the COVID-19 Virus Before Labor Increase the Rate of MSAF and Affect Maternal and Fetal Outcomes?","authors":"Runrun Feng, Yu Tao, Haiyan Sun, Cen Cao, Hairong Gu, Junmei Hu, Wenwen Chang, Xia Li, Ziyan Jiang","doi":"10.1155/2024/8456910","DOIUrl":"10.1155/2024/8456910","url":null,"abstract":"<p><p><b>Objective:</b> The main objective of this study is to investigate whether there is a difference in the occurrence of MSAF between fever and nonfever-pregnant women during the COVID-19 pandemic. <b>Study Design:</b> We conducted a multicenter retrospective study including pregnant women during the COVID-19 pandemic. Among the 400 pregnant women included in the final data analysis, 238 had fever during delivery, while 162 nonfever-pregnant women met the inclusion and exclusion criteria and served as controls. We collected various obstetric and neonatal parameters for both groups of patients, compared, and statistically tested the significance of these parameters. For parameters with significant statistical differences and clinical significance, we performed logistic regression analysis to explore potential risk factors for MSAF. <b>Result:</b> In a sample of 400 parturients, a total of 69 individuals (17.25%) were discovered to have MSAF with the prevalence increasing to 21.85% in the fever population. A statistically significant association was observed between fever during pregnancy and MSAF, with a higher risk of development observed in fever-pregnant women compared to nonfever ones. Specifically, the odds of developing MSAF increased by a factor of 0.979 in fever-pregnant women compared to nonfever ones, as determined by a logistic regression model (OR = 1.979, 95% CI = 1.061∼3.693, <i>p</i>=0.032). Moreover, pregnant women with COVID-19 infection had a significantly higher risk of developing MSAF, with the odds increasing by a factor of 2.567 compared to uninfected pregnant women (OR = 3.567, 95% CI = 1.622∼7.845, <i>p</i>=0.002). In addition, the study also identified abnormal fetal heart monitoring (<i>p</i> < 0.05) and gestational age (<i>p</i> < 0.05) as independent risk factors for the occurrence of MSAF. <b>Conclusion:</b> For pregnant women infected with COVID-19, the rate of MSAF disturbance significantly increases, therefore, it is necessary to pay more attention to fetal heart changes and amniotic fluid conditions, and actively managing labor is beneficial for improving delivery outcomes.</p>","PeriodicalId":50715,"journal":{"name":"Canadian Journal of Infectious Diseases & Medical Microbiology","volume":"2024 ","pages":"8456910"},"PeriodicalIF":2.6,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11573438/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142669782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phage Therapy Against Antibiotic-Resistant and Multidrug-Resistant Infections Involving Nonhealing Wounds and Prosthetic Joint Infections Associated With Biofilms: A Mini-Review.","authors":"Kashif Haq, Martin Figgitt, David Lee","doi":"10.1155/2024/6252415","DOIUrl":"10.1155/2024/6252415","url":null,"abstract":"<p><p>Chronic wounds and prosthetic joint infections are difficult to treat and are associated with a high burden of disease and economic cost. The rise of antibiotic resistance and the understanding of biofilm formation has inflamed an already challenging situation. Bacteriophage therapy has been used throughout the last century to treat bacterial infections. However, in the last 10 years, there has been a resurgence in phage therapy as a novel innovative treatment for nonhealing wounds. This mini systemic review assesses relevant clinical studies, case series and trials over 5 years associated with safety, treatment and success rates of phage therapy concerning nonhealing and prosthetic joint infections. A search of PubMed, Web of Science, Cochrane and Clinical Trials.gov databases resulted in 3151 studies, 27 met the criteria, and a total of 152 bacterial infections were treated from 130 individuals. Most common pathogen isolated in wounds was <i>P. aeruginosa</i>, and <i>S. aureus</i> was mostly associated with prosthetic joint infections. Treatment modalities differed across studies, adverse effects were limited, and success rate was deemed to be 91%.</p>","PeriodicalId":50715,"journal":{"name":"Canadian Journal of Infectious Diseases & Medical Microbiology","volume":"2024 ","pages":"6252415"},"PeriodicalIF":2.6,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11563716/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142631967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Healthcare-Associated Infections' Characteristics Among Burn Patients and Risk Factors of Mortality: A Study Based on Data From a Tertiary Center in Iran: Nosocomial Infections Among Burn Patients.","authors":"Nima Mozafari, Effat Abbasi Montazeri, Sasan Moogahi, Seyed Mohammad Amin Alavi","doi":"10.1155/2024/8707245","DOIUrl":"10.1155/2024/8707245","url":null,"abstract":"<p><p><b>Background:</b> Burn patients are more likely to get healthcare-associated infections (HAIs). The current study aimed to investigate the characteristics of HAI and mortality risk factors among burn patients admitted to a tertiary center in Iran. <b>Methods:</b> A retrospective study was conducted in 2021 on burn patients who developed HAI after hospitalization in a tertiary center in Ahvaz, Iran. The records of patients admitted and managed between March 2019 and March 2020 were reviewed. Statistical analysis was carried out using IBM SPSS Version 26, with <i>p</i> < 0.05 considered statistically significant. <b>Results:</b> Of the 1,659 admitted patients, 395 (23.8%) developed HAI during the study period. After excluding patients with incomplete medical records and those with fungal or viral infections, 363 patients remained. The majority of these cases occurred in male patients. The most common type of HAI was burn wound infection (56.2%). Patients were mainly affected by Gram-negative bacteria, <i>Pseudomonas aeruginosa</i> (39.7%), while the most common Gram-positive bacteria was <i>Staphylococcus epidermididis</i> (9.6%). The most common antibiotic resistance among <i>P. aeruginosa</i>-infected patients was reported against imipenem, followed by gentamicin and ciprofloxacin; however, the mentioned organism was mainly sensitive to colistin. Gender, age, bloodstream infection (BSI), ventilator-associated infection (VAI), ICU admission, and total burned surface area (TBSA) resulted in 3.585, 1.028, 2.222, 7.469, 5.278-, and 1.031 times higher mortality rates, respectively. <b>Conclusion:</b> Female gender, advanced age, BSI, VAI, and ICU admission are risk factors for HAI. These findings emphasize the need for focused infection prevention and management to improve high-risk burn patient survival.</p>","PeriodicalId":50715,"journal":{"name":"Canadian Journal of Infectious Diseases & Medical Microbiology","volume":"2024 ","pages":"8707245"},"PeriodicalIF":2.6,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11535417/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142584848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Haematological Profile and Antibiotic Resistance of Bacteria Responsible for Enteric Infections Isolated From Patients Suffering From Malaria and Enteric Infections on Consultation at the Dschang Regional Hospital.","authors":"Roland Y Ngai, Wiliane J T Marbou, Armelle T Mbaveng, Victor Kuete","doi":"10.1155/2024/3383995","DOIUrl":"10.1155/2024/3383995","url":null,"abstract":"<p><p>Malarial and bacterial coinfections in low-income countries are a serious cause of morbidity and mortality, necessitating coadministration of antibiotics and antimalarials. This study investigated the relationship between malaria infection and bacterial drug resistance in malaria and nonmalaria patients on consultation at the Dschang Regional Hospital. A follow-up study was carried out from October 2020 to December 2021 on 127 malaria and 174 nonmalaria patients having enteric infections. Clinical and haematological parameters were measured using standard methods. CD4 and CD8 cells were determined using flow cytometry. Enteric bacteria pathogens were isolated from stool, and antimicrobial and antimalarial profiles were determined using agar diffusion and microdilution methods, respectively. Significant reduction of RBCs, WBCs, CD4, CD8, granulocytes, monocytes and platelets was seen in coinfected patients compared to monoinfected participants (<i>p</i> ≤ 0.0491). <i>E. coli</i> was the main pathogenic bacteria isolated from the digestive tract of coinfected patients (40.63%) and monoinfected patients (59.37%). <i>E. coli</i> showed a high level of resistance to AMX (57.69%) and CDA (61.54%) in coinfected patients compared to 55.26% and 41.67%, respectively, in monoinfected patients. Quinine (53[50.00%]; 6[42.86%]) presented a minimal inhibitory concentration (MIC) of 32 μg/mL on the bacteria isolates from coinfected and monoinfected patients, respectively, while Artemether 89 (83.96%), Maloxine 5 (3.94%) and Surquina 250 (39.37%) presented a MIC of 64 μg/mL on bacterial isolates of coinfected and monoinfected patients. <i>E. coli</i> showed high resistance against AKI (45.93%), AMX (43.75%) and ERY (59.37%) in malaria patients who were under antimalarial drugs compared to malaria patients who were not under malaria drugs (29.68%, 34.37% and 32.81%, respectively). This study highlights that antimalarial drugs might certainly have an influence on the acquisition and emergence of bacterial resistance in the case of malaria bacterial coinfection, and therefore, adequate management and planning effective control programmes might certainly go a long way to reduce the rate of morbidity and mortality.</p>","PeriodicalId":50715,"journal":{"name":"Canadian Journal of Infectious Diseases & Medical Microbiology","volume":"2024 ","pages":"3383995"},"PeriodicalIF":2.6,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11530289/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142570353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}