Canadian Journal of Infectious Diseases & Medical Microbiology最新文献

{"title":"Morphological Characterization and Genotyping of <i>Acanthamoeba</i> Isolates From Oral and Nasal Samples of Cancer Patients in Kashan, Iran.","authors":"Sima Rasti, Tayebeh Taghipour, Mahdi Delavari, Hossein Hooshyar, Gholam Abbas Moosavi, Mohsen Arbabi","doi":"10.1155/2024/4071707","DOIUrl":"10.1155/2024/4071707","url":null,"abstract":"<p><p><b>Background:</b> <i>Acanthamoeba</i> species are recognized as the most prevalent free-living amoebae (FLA). They can cause granulomatous amebic encephalitis (GAE) and pulmonary and ocular infections. The present study aimed to isolate and identify <i>Acanthamoeba</i> genotypes in cancer patients referred to Kashan's hospitals in Central Iran. <b>Methods:</b> This cross-sectional study was conducted with oral and nasal swab samples collected from a hundred cancer patients referred to Kashan's Beheshti and Yasrebi hospitals during 2019-2020. The samples were cultured in 1.5% non-nutrient agar (NNA) with heat-killed <i>Escherichia coli</i> and examined for \"FLA.\" A polymerase chain reaction (PCR) assay amplifying the 18S rRNA gene was performed, and <i>Acanthamoeba</i>-positive isolates were subjected to nucleotide sequencing to identify their genotypes. <b>Results:</b> The prevalence of <i>Acanthamoeba</i> infection was 51% in the oral cavity and 38% in the nasal samples of cancer patients. The most frequent <i>Acanthamoeba</i> cysts were (51.3%) wrinkled polygonal and sized 9.55-11.5 μm (Group II). <i>Acanthamoeba</i> genotypes T4, T11, and T5 were identified in the oral cavity samples, whereas T4 and T11 were detected in the nasal samples. <b>Conclusion:</b> The prevalence of <i>Acanthamoeba</i> infection in oral and nasal cancer patients was higher in Kashan, Iran, compared to other countries. Due to the high rate of oral <i>Acanthamoeba</i> contamination, oral sampling is recommended for better detection of this protozoan. Since T4 is the predominant genotype that can cause serious diseases in high-risk groups, increasing physicians' awareness of infections associated with <i>Acanthamoeba</i> and preventive and control measures are strongly suggested.</p>","PeriodicalId":50715,"journal":{"name":"Canadian Journal of Infectious Diseases & Medical Microbiology","volume":"2024 ","pages":"4071707"},"PeriodicalIF":2.6,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11578656/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142683167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does Fever Caused by the COVID-19 Virus Before Labor Increase the Rate of MSAF and Affect Maternal and Fetal Outcomes?","authors":"Runrun Feng, Yu Tao, Haiyan Sun, Cen Cao, Hairong Gu, Junmei Hu, Wenwen Chang, Xia Li, Ziyan Jiang","doi":"10.1155/2024/8456910","DOIUrl":"10.1155/2024/8456910","url":null,"abstract":"<p><p><b>Objective:</b> The main objective of this study is to investigate whether there is a difference in the occurrence of MSAF between fever and nonfever-pregnant women during the COVID-19 pandemic. <b>Study Design:</b> We conducted a multicenter retrospective study including pregnant women during the COVID-19 pandemic. Among the 400 pregnant women included in the final data analysis, 238 had fever during delivery, while 162 nonfever-pregnant women met the inclusion and exclusion criteria and served as controls. We collected various obstetric and neonatal parameters for both groups of patients, compared, and statistically tested the significance of these parameters. For parameters with significant statistical differences and clinical significance, we performed logistic regression analysis to explore potential risk factors for MSAF. <b>Result:</b> In a sample of 400 parturients, a total of 69 individuals (17.25%) were discovered to have MSAF with the prevalence increasing to 21.85% in the fever population. A statistically significant association was observed between fever during pregnancy and MSAF, with a higher risk of development observed in fever-pregnant women compared to nonfever ones. Specifically, the odds of developing MSAF increased by a factor of 0.979 in fever-pregnant women compared to nonfever ones, as determined by a logistic regression model (OR = 1.979, 95% CI = 1.061∼3.693, <i>p</i>=0.032). Moreover, pregnant women with COVID-19 infection had a significantly higher risk of developing MSAF, with the odds increasing by a factor of 2.567 compared to uninfected pregnant women (OR = 3.567, 95% CI = 1.622∼7.845, <i>p</i>=0.002). In addition, the study also identified abnormal fetal heart monitoring (<i>p</i> < 0.05) and gestational age (<i>p</i> < 0.05) as independent risk factors for the occurrence of MSAF. <b>Conclusion:</b> For pregnant women infected with COVID-19, the rate of MSAF disturbance significantly increases, therefore, it is necessary to pay more attention to fetal heart changes and amniotic fluid conditions, and actively managing labor is beneficial for improving delivery outcomes.</p>","PeriodicalId":50715,"journal":{"name":"Canadian Journal of Infectious Diseases & Medical Microbiology","volume":"2024 ","pages":"8456910"},"PeriodicalIF":2.6,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11573438/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142669782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phage Therapy Against Antibiotic-Resistant and Multidrug-Resistant Infections Involving Nonhealing Wounds and Prosthetic Joint Infections Associated With Biofilms: A Mini-Review.","authors":"Kashif Haq, Martin Figgitt, David Lee","doi":"10.1155/2024/6252415","DOIUrl":"10.1155/2024/6252415","url":null,"abstract":"<p><p>Chronic wounds and prosthetic joint infections are difficult to treat and are associated with a high burden of disease and economic cost. The rise of antibiotic resistance and the understanding of biofilm formation has inflamed an already challenging situation. Bacteriophage therapy has been used throughout the last century to treat bacterial infections. However, in the last 10 years, there has been a resurgence in phage therapy as a novel innovative treatment for nonhealing wounds. This mini systemic review assesses relevant clinical studies, case series and trials over 5 years associated with safety, treatment and success rates of phage therapy concerning nonhealing and prosthetic joint infections. A search of PubMed, Web of Science, Cochrane and Clinical Trials.gov databases resulted in 3151 studies, 27 met the criteria, and a total of 152 bacterial infections were treated from 130 individuals. Most common pathogen isolated in wounds was <i>P. aeruginosa</i>, and <i>S. aureus</i> was mostly associated with prosthetic joint infections. Treatment modalities differed across studies, adverse effects were limited, and success rate was deemed to be 91%.</p>","PeriodicalId":50715,"journal":{"name":"Canadian Journal of Infectious Diseases & Medical Microbiology","volume":"2024 ","pages":"6252415"},"PeriodicalIF":2.6,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11563716/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142631967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Healthcare-Associated Infections' Characteristics Among Burn Patients and Risk Factors of Mortality: A Study Based on Data From a Tertiary Center in Iran: Nosocomial Infections Among Burn Patients.","authors":"Nima Mozafari, Effat Abbasi Montazeri, Sasan Moogahi, Seyed Mohammad Amin Alavi","doi":"10.1155/2024/8707245","DOIUrl":"10.1155/2024/8707245","url":null,"abstract":"<p><p><b>Background:</b> Burn patients are more likely to get healthcare-associated infections (HAIs). The current study aimed to investigate the characteristics of HAI and mortality risk factors among burn patients admitted to a tertiary center in Iran. <b>Methods:</b> A retrospective study was conducted in 2021 on burn patients who developed HAI after hospitalization in a tertiary center in Ahvaz, Iran. The records of patients admitted and managed between March 2019 and March 2020 were reviewed. Statistical analysis was carried out using IBM SPSS Version 26, with <i>p</i> < 0.05 considered statistically significant. <b>Results:</b> Of the 1,659 admitted patients, 395 (23.8%) developed HAI during the study period. After excluding patients with incomplete medical records and those with fungal or viral infections, 363 patients remained. The majority of these cases occurred in male patients. The most common type of HAI was burn wound infection (56.2%). Patients were mainly affected by Gram-negative bacteria, <i>Pseudomonas aeruginosa</i> (39.7%), while the most common Gram-positive bacteria was <i>Staphylococcus epidermididis</i> (9.6%). The most common antibiotic resistance among <i>P. aeruginosa</i>-infected patients was reported against imipenem, followed by gentamicin and ciprofloxacin; however, the mentioned organism was mainly sensitive to colistin. Gender, age, bloodstream infection (BSI), ventilator-associated infection (VAI), ICU admission, and total burned surface area (TBSA) resulted in 3.585, 1.028, 2.222, 7.469, 5.278-, and 1.031 times higher mortality rates, respectively. <b>Conclusion:</b> Female gender, advanced age, BSI, VAI, and ICU admission are risk factors for HAI. These findings emphasize the need for focused infection prevention and management to improve high-risk burn patient survival.</p>","PeriodicalId":50715,"journal":{"name":"Canadian Journal of Infectious Diseases & Medical Microbiology","volume":"2024 ","pages":"8707245"},"PeriodicalIF":2.6,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11535417/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142584848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Haematological Profile and Antibiotic Resistance of Bacteria Responsible for Enteric Infections Isolated From Patients Suffering From Malaria and Enteric Infections on Consultation at the Dschang Regional Hospital.","authors":"Roland Y Ngai, Wiliane J T Marbou, Armelle T Mbaveng, Victor Kuete","doi":"10.1155/2024/3383995","DOIUrl":"10.1155/2024/3383995","url":null,"abstract":"<p><p>Malarial and bacterial coinfections in low-income countries are a serious cause of morbidity and mortality, necessitating coadministration of antibiotics and antimalarials. This study investigated the relationship between malaria infection and bacterial drug resistance in malaria and nonmalaria patients on consultation at the Dschang Regional Hospital. A follow-up study was carried out from October 2020 to December 2021 on 127 malaria and 174 nonmalaria patients having enteric infections. Clinical and haematological parameters were measured using standard methods. CD4 and CD8 cells were determined using flow cytometry. Enteric bacteria pathogens were isolated from stool, and antimicrobial and antimalarial profiles were determined using agar diffusion and microdilution methods, respectively. Significant reduction of RBCs, WBCs, CD4, CD8, granulocytes, monocytes and platelets was seen in coinfected patients compared to monoinfected participants (<i>p</i> ≤ 0.0491). <i>E. coli</i> was the main pathogenic bacteria isolated from the digestive tract of coinfected patients (40.63%) and monoinfected patients (59.37%). <i>E. coli</i> showed a high level of resistance to AMX (57.69%) and CDA (61.54%) in coinfected patients compared to 55.26% and 41.67%, respectively, in monoinfected patients. Quinine (53[50.00%]; 6[42.86%]) presented a minimal inhibitory concentration (MIC) of 32 μg/mL on the bacteria isolates from coinfected and monoinfected patients, respectively, while Artemether 89 (83.96%), Maloxine 5 (3.94%) and Surquina 250 (39.37%) presented a MIC of 64 μg/mL on bacterial isolates of coinfected and monoinfected patients. <i>E. coli</i> showed high resistance against AKI (45.93%), AMX (43.75%) and ERY (59.37%) in malaria patients who were under antimalarial drugs compared to malaria patients who were not under malaria drugs (29.68%, 34.37% and 32.81%, respectively). This study highlights that antimalarial drugs might certainly have an influence on the acquisition and emergence of bacterial resistance in the case of malaria bacterial coinfection, and therefore, adequate management and planning effective control programmes might certainly go a long way to reduce the rate of morbidity and mortality.</p>","PeriodicalId":50715,"journal":{"name":"Canadian Journal of Infectious Diseases & Medical Microbiology","volume":"2024 ","pages":"3383995"},"PeriodicalIF":2.6,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11530289/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142570353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial Analysis of the Whole Blood in Chinese Healthy Donors Using 16S rDNA-Targeted Metagenomic Sequencing.","authors":"Jingjing Zhang, Yanmin He, Chen Chen, Wei Hu, Ji He, Yanling Ying, Faming Zhu","doi":"10.1155/2024/6635560","DOIUrl":"https://doi.org/10.1155/2024/6635560","url":null,"abstract":"<p><p><b>Background:</b> The presence of bacteria in the blood of healthy individuals remains controversial. This study explored the comprehensive bacterial profiles and specific biomarkers in different components of healthy Chinese blood donors. <b>Methods:</b> A total of 5230 whole blood (WB) specimens were collected. Among them, 5200 random samples were pooled into 26 mixed samples for bacterial profile analysis. The remaining 30 random samples were divided into 4 groups based on components: WB, plasma, red blood cells (RBCs), and buffy coat (BC). Subsequently, the amplicons of the bacterial 16S rDNA V3-V4 fragments were sequenced to measure the diversity and composition of the bacteria using next-generation sequencing. <b>Results:</b> The bacterial DNAs in the blood primarily originated from the Proteobacteria phylum. A total of 301 species of bacterial DNA were found in blood specimens, with 46 species being present among all groups. A significantly higher abundance of bacterial DNA was found in the plasma and RBCs compared to those in BC and WB. However, the plasma and RBC groups showed significantly higher species diversity and richness compared to the BC and WB groups. In addition, the WB group had a significantly different community structure and composition compared to the plasma and RBC groups but was similar to the BC group. <b>Conclusion:</b> The presence of bacterial DNA fragments was confirmed in blood from healthy Chinese donors. The bacterial DNA fragments enriched in plasma showed the highest diversity, followed by RBC, WB, and BC. These results provide a foundation for further research on the microbiome in the blood of healthy individuals.</p>","PeriodicalId":50715,"journal":{"name":"Canadian Journal of Infectious Diseases & Medical Microbiology","volume":"2024 ","pages":"6635560"},"PeriodicalIF":2.6,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11498981/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142512364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Rare but Not so Rare? The Evolving Spectrum of Whipple's Disease\".","authors":"John M Conly, B Lynn Johnston","doi":"10.1155/2024/9809135","DOIUrl":"10.1155/2024/9809135","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1155/2001/547516.].</p>","PeriodicalId":50715,"journal":{"name":"Canadian Journal of Infectious Diseases & Medical Microbiology","volume":"2024 ","pages":"9809135"},"PeriodicalIF":2.6,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11489003/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142479878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and Bacterial Characteristics of Bloodstream Infections Caused by <i>Listeria monocytogenes</i> in Western China.","authors":"Nan Wang, Liuqing Yang, Yu Yuan, Chongyang Wu, Chao He","doi":"10.1155/2024/7785327","DOIUrl":"10.1155/2024/7785327","url":null,"abstract":"<p><strong>Objective: </strong>Bloodstream infections (BSIs) caused by <i>Listeria monocytogenes</i> are linked to high mortality of the patients. Case-specific details related to this disease and causative strains in different districts remain to be characterized.</p><p><strong>Methods: </strong>In this study, medical data of BSIs admitted to West China Hospital from October 2017 to March 2023 were retrieved from the hospital information system. The <i>in vitro</i> antimicrobial susceptibility testing and whole-genome sequencing were performed for <i>L. monocytogenes</i> strains isolated from blood specimens. The genetic relationship of these strains with those in public databases was also analyzed.</p><p><strong>Result: </strong>The in-hospital mortality of <i>L. monocytogenes</i> BSIs was 25.7% (9/35). The changes in consciousness and elevated serum C-reactive protein (CRP) level were found to be the differential factors of <i>L. monocytogenes</i> BSIs (<i>P</i> < 0.05). All the 27 strains studied were susceptible to ampicillin, meropenem, and erythromycin. Only 22.2% of them were susceptible to trimethoprim-sulfamethoxazole. The <i>Listeria</i> pathogenicity islands 1 (LIPI-1), truncated LIPI-2, and multiple virulence-related genes outside the LIPIs were determined from these strains. Also, 12 sequence types (STs) and 12 clonal complexes (CCs) were identified and classified into clonal lineages I (9/27, 33.3%) and lineages II (18/27, 66.7%), demonstrating genetic differences with the strains in the database. ST451/CC11 (5/27, 18.5%) and ST8/CC8 (4/27, 14.8%) were the common genotypes.</p><p><strong>Conclusions: </strong>The consciousness change and elevated serum CRP level were found to be the differential factors of <i>L. monocytogenes</i> BSIs. Considering the high virulence of the strains, it is needed to pay more attention to the dissemination of the predominant genotype.</p>","PeriodicalId":50715,"journal":{"name":"Canadian Journal of Infectious Diseases & Medical Microbiology","volume":"2024 ","pages":"7785327"},"PeriodicalIF":2.6,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11452242/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142382230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal Drug Susceptibility Profile of <i>Candida kefyr</i> Isolated from Clinical Samples and Dairy Products.","authors":"Zahra Zareshahrabadi, Samin Khaliji, Maryam Roudbari, Kamiar Zomorodian","doi":"10.1155/2024/6594366","DOIUrl":"10.1155/2024/6594366","url":null,"abstract":"<p><p>Exploring drug susceptibility is a critical endeavor in the scientific community, setting the stage for advancements in understanding and combating various pathogens. <i>Candida kefyr</i> has emerged as a significant pathogen, particularly affecting immunocompromised individuals with hematologic malignancies and HIV/AIDS conditions. This study aimed to assess the antifungal susceptibility profile of <i>Candida kefyr</i> isolates obtained from clinical samples and dairy products. A total of 134 <i>Candida kefyr</i> yeast isolates were retrieved from three distinct groups: (1) healthy individuals (<i>n</i> = 41), (2) patients (<i>n</i> = 24) including hematologic malignancy (<i>n</i> = 9), HIV/AIDS (<i>n</i> = 7), and diabetes (<i>n</i> = 8), (3) dairy products (milk, yogurt, and cheese, <i>n</i> = 69) stored at -70°C in the Shiraz University of Medical Science. All <i>Candida kefyr</i> isolates were previously identified using conventional and molecular methods. Susceptibility to antifungal drugs, including caspofungin, fluconazole, itraconazole, voriconazole, and amphotericin B, was determined using the microdilution method following CLSI-M27-A3 protocols, with results interpreted according to CLSI-M27-S4 guidelines. The study emphasizes the clear variation in antifungal susceptibility testing of <i>Candida kefyr</i> strains when compared across different groups, including patients, healthy people, and dairy products. According to the results, across all groups, a high minimum inhibitory concentration of fluconazole is evident, and healthy individuals show the highest minimum inhibitory concentration geometric means (4.0681). Also, 79.1% of the isolates were wild type to amphotericin B, with the lowest minimum inhibitory concentration compared to other antifungals tested. This suggests that amphotericin B was more effective against <i>Candida kefyr</i>. These findings showed fewer susceptibilities of <i>Candida kefyr</i> to both triazole and echinocandin classes of antifungal agents. Additionally, it is noteworthy that individuals without medical conditions exhibited higher minimum inhibitory concentration rates to these antifungal agents in comparison to those with underlying health conditions. Consequently, timely diagnosis and appropriate therapeutic interventions emerge as imperative in the effective management of candidiasis cases.</p>","PeriodicalId":50715,"journal":{"name":"Canadian Journal of Infectious Diseases & Medical Microbiology","volume":"2024 ","pages":"6594366"},"PeriodicalIF":2.6,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11442036/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142331722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Virulence Genotypes, Antimicrobial Resistance Patterns, and Biofilm Synthesis in <i>Salmonella</i> spp Isolated from Foodborne Outbreaks.","authors":"Mohammad Mehdi Soltan Dallal, Ahmad Nasser, Samira Karimaei","doi":"10.1155/2024/4805228","DOIUrl":"https://doi.org/10.1155/2024/4805228","url":null,"abstract":"<p><p><i>Salmonella</i> is the main bacterial pathogen that causes foodborne disease, particularly in developing countries. Nontyphoidal <i>Salmonella</i> (NTS) include <i>Enteritidis</i> and <i>Typhimurium</i> as the most prevalent strains which are one of the significant causes of acute gastroenteritis in children. Therefore, identifying the most predominant serovars, types of common contaminated food, and paying attention to their antibiotic resistance are the main factors in the prevention and control strategy of salmonellosis. This study was undertaken to evaluate the prevalence rate of serovars, the biofilm formation, antimicrobial resistance (AMR) status, and phenotypic virulence factors of <i>Salmonella</i> strains isolated from diarrhea samples in some cities of Iran. A total of 40 (10.41%) <i>Salmonella</i> isolates were recovered from 384 diarrhea samples processed and the most common serovar was <i>Salmonella</i> serovar <i>Typhimurium</i> (82.5). Also, all isolates belonging to serovar <i>Typhimurium</i> showed more virulence factors compared to other serovars. The isolates showed a high resistance rate to ampicillin (95%) and nalidixic acid (87.5%), while a low resistance rate was found for chloramphenicol (2.5%). Moreover, significant variances in the capacity of biofilm formation were found between different <i>Salmonella</i> serotypes. The resistance of NTS to extant choice drugs is a potential public health problem. Constant monitoring of AMR pattern and virulence profile of NTS serovars is suggested for the prevention of salmonellosis in humans.</p>","PeriodicalId":50715,"journal":{"name":"Canadian Journal of Infectious Diseases & Medical Microbiology","volume":"2024 ","pages":"4805228"},"PeriodicalIF":2.6,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11436275/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142331723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}