A Critical Review of the CRISPR-Cas Technology in the Detection of SARS-CoV-2 Variants.

IF 2.6 4区医学 Q3 INFECTIOUS DISEASES

Canadian Journal of Infectious Diseases & Medical Microbiology Pub Date : 2025-07-09 eCollection Date: 2025-01-01 DOI:10.1155/cjid/9107724

Jie Zhang, Juezhuo Li, Jiawei Zhou, Jiaye Zhong, Yue Xu, Xiaolei Mao, Minghui Xu, Shuyin Luo, Yi Yang, Ruiyao Hu, Dong-Ang Liu, Shiyu Chen, Yuting Qiu, Keyi Chen, Jinghua Yuan, Xinling Zhang, Xiaoping Li

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引用次数: 0

Abstract

Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is still going on, and as the epidemic situation continues, the genome of SARS-CoV-2 is also mutating and evolving, resulting in more and more SARS-CoV-2 mutant strains, which have brought serious pressure on the prevention and control of COVID-19. Given that the COVID-19 is still spreading, it is extremely important to rapidly identify SARS-CoV-2 variants by nucleic acid assays. Thus, developing highly sensitive and specific assays that are suitable for field testing, high-throughput, and automation, as well as other diagnostic applications for SARS-CoV-2 variants, is urgently needed. This paper reviews the research progress of novel CRISPR-based diagnostic methods for SARS-CoV-2 variants.

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CRISPR-Cas技术检测SARS-CoV-2变体的综述

由严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）引起的2019冠状病毒病（COVID-19）仍在继续，随着疫情的持续，SARS-CoV-2基因组也在发生突变和进化，导致SARS-CoV-2突变株越来越多，这给COVID-19的防控带来了严重的压力。鉴于COVID-19仍在传播，通过核酸检测快速识别SARS-CoV-2变体至关重要。因此，迫切需要开发适用于现场测试、高通量和自动化以及其他诊断应用的高灵敏度和特异性检测方法。本文综述了基于crispr的新型SARS-CoV-2变异诊断方法的研究进展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Canadian Journal of Infectious Diseases & Medical Microbiology 医学-传染病学

CiteScore

4.30

自引率

0.00%

发文量

108

审稿时长

>12 weeks

期刊介绍： Canadian Journal of Infectious Diseases and Medical Microbiology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies related to infectious diseases of bacterial, viral and parasitic origin. The journal welcomes articles describing research on pathogenesis, epidemiology of infection, diagnosis and treatment, antibiotics and resistance, and immunology.