Journal of Cytology最新文献_第7页

A Case of Cutaneous Leishmaniasis: An Emphasis on Scrape Cytology as a Diagnostic Modality. 皮肤利什曼病1例：强调刮擦细胞学作为诊断方法。

IF 1 4区医学

Journal of Cytology Pub Date : 2025-04-01 Epub Date: 2025-05-29 DOI: 10.4103/joc.joc_199_23

Ananya Palanisamy, Debasis Gochhait, Mithraa Devi Sekar, Siddaraju Neeliah

引用次数: 0

Role of Immunocytochemistry in Detection of Mycobacterium tuberculosis Antigen by Fine Needle Aspiration Cytology. 免疫细胞化学在细针吸细胞学检测结核分枝杆菌抗原中的作用。

IF 1 4区医学

Journal of Cytology Pub Date : 2025-04-01 Epub Date: 2025-05-29 DOI: 10.4103/joc.joc_37_24

Pampa Ch Toi, Apoorvalakshmi Subramaniam, Noyal Mariya Joseph, Neelaiah Siddaraju

{"title":"Role of Immunocytochemistry in Detection of Mycobacterium tuberculosis Antigen by Fine Needle Aspiration Cytology.","authors":"Pampa Ch Toi, Apoorvalakshmi Subramaniam, Noyal Mariya Joseph, Neelaiah Siddaraju","doi":"10.4103/joc.joc_37_24","DOIUrl":"10.4103/joc.joc_37_24","url":null,"abstract":"Background: Detection of extrapulmonary tuberculosis (EPTB) by Ziehl-Neelsen (Z-N) staining in fine needle aspirates is challenging due to the low yield of acid-fast bacteria (AFB). Mycobacterial culture, the gold standard, takes 4-8 weeks. Polymerase chain reaction with 100% sensitivity and 92.1% specificity is expensive. Mycobacterial antigens produced by the tubercle bacilli consist of several proteins and enzymes. The protein purified from Mycobacterium tuberculosis is called MPT. The MPT64, a 24-kd protein, has not been detected in non-tuberculous mycobacteria. We aim to study the role of immunocytochemical (ICC) in the detection of EPTB in fine needle aspiration cytology materials by using MPT64 antibody and compare it with culture and Z-N staining, as ICC is not routinely practiced for diagnosing EPTB.Materials and methods: A total of 134 patients having enlarged nodes with suspected EPTB were included; however, only 96/134 cases were suitable for statistical analysis. Papanicolaou, May-Grünwald-Giemsa, Z-N staining, ICC, and mycobacteria culture were performed.Results: AFB was positive in 16%, 22.9% of culture-positive, and 11% of MPT64 positive. The sensitivity and specificity of ICC compared to mycobacterial culture were 45.4% and 99%, respectively. ICC and culture had a moderate agreement with the Kappa value of 0.535. The positive and negative predictive values of ICC with culture were 91% and 86%, respectively.Conclusion: This study tried to improve the technique's sensitivity to facilitate its use in routine laboratory practice. Nonetheless, our results showed no significant improvement over the currently popular Z-N stain, although a comparison between the ICC technique performed on smears and cell block sections showed better results in the latter.","PeriodicalId":50217,"journal":{"name":"Journal of Cytology","volume":"42 2","pages":"82-87"},"PeriodicalIF":1.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12165620/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144303488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Solid Pseudopapillary Neoplasm in a 10-Year-Old Boy Diagnosed by Endoscopic Ultrasound-Fine-Needle Aspiration (EUS-FNA). 10岁男童经超声细针穿刺（EUS-FNA）诊断为实性假乳头状肿瘤。

IF 1 4区医学

Journal of Cytology Pub Date : 2025-04-01 Epub Date: 2025-05-29 DOI: 10.4103/joc.joc_188_23

Buse Aki, Senay Erdogan-Durmus, Ozben Yalcin, Refik Selim Atamanalp

引用次数: 0

Endobronchial Ultrasound-Guided Transbronchial Needle Aspiration Cytology: Navigating the Diagnostic Challenge between Tuberculosis and Sarcoidosis in Endemic Regions. 支气管超声引导下经支气管针吸细胞学：在结核病和结节病流行地区之间导航诊断挑战。

IF 1 4区医学

Journal of Cytology Pub Date : 2025-04-01 Epub Date: 2025-05-29 DOI: 10.4103/joc.joc_123_24

Tanvi Jha, Prajwala Gupta, Desh Deepak

{"title":"Endobronchial Ultrasound-Guided Transbronchial Needle Aspiration Cytology: Navigating the Diagnostic Challenge between Tuberculosis and Sarcoidosis in Endemic Regions.","authors":"Tanvi Jha, Prajwala Gupta, Desh Deepak","doi":"10.4103/joc.joc_123_24","DOIUrl":"10.4103/joc.joc_123_24","url":null,"abstract":"Objective: Endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) plays an important diagnostic role in concealed pulmonary diseases. However, diagnosing sarcoidosis and differentiating it from tuberculosis is a diagnostic quandary. We, thus, aimed to evaluate EBUS-TBNA cytology in these cases in a tubercular-endemic zone.Methods: A prospective 5-year study was done in a tertiary care center on 118 patients with tuberculosis versus sarcoidosis with mediastinal lymphadenopathy who underwent EBUS-TBNA. All samples obtained were sent for cytomorphological and microbiological evaluation. On cytology analysis, demonstration of acid-fast bacilli was considered diagnostic of tuberculosis. However, in its absence, a multidisciplinary diagnostic (MDD) approach was followed to establish a diagnosis.Results: EBUS-TBNA cytology contributed in reaching the final diagnosis in 88.1% cases. Of the 55 cases of tuberculosis, cytomorphological features were contributory in 90.9% cases. Out of 29 cases of sarcoidosis, 24 showed granulomas. Microbiological tests were contributory in the final diagnosis of tuberculosis in only 21.8% cases as compared to 90.9% by cytology analysis. In 10 cases, a definitive diagnosis could not be made on cytology analysis and were finally diagnosed on the basis of MDD. A definitive distinction between tuberculosis and sarcoidosis could not be made despite MDD in four cases.Conclusion: While a diagnosis of tuberculosis can be made independently on cytological features, MDD with contributory cytological findings is essential for the diagnosis of sarcoidosis. EBUS-TBNA cytology, thus, plays an important role in the multidisciplinary strategy of approaching thoracic lesions.","PeriodicalId":50217,"journal":{"name":"Journal of Cytology","volume":"42 2","pages":"67-74"},"PeriodicalIF":1.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12165614/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144303473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role of Immunohistochemistry in Reducing the "Atypia of Undetermined Significance (AUS)" Category in Serous Fluid Cytology. 免疫组织化学在减少“不确定意义异型性”（AUS）中的作用浆液细胞学分类。

IF 1 4区医学

Journal of Cytology Pub Date : 2025-01-01 Epub Date: 2025-02-11 DOI: 10.4103/joc.joc_212_23

Enver Yarikkaya, Senay Erdogan-Durmus

{"title":"The Role of Immunohistochemistry in Reducing the \"Atypia of Undetermined Significance (AUS)\" Category in Serous Fluid Cytology.","authors":"Enver Yarikkaya, Senay Erdogan-Durmus","doi":"10.4103/joc.joc_212_23","DOIUrl":"10.4103/joc.joc_212_23","url":null,"abstract":"Background and aims: The International Serous Fluid Cytopathology Reporting System aimed to establish standardized protocols to ensure consistency in the reporting of serous fluid cytological specimens. In the search for higher diagnostic accuracy and a reduction in indeterminate categories, such as atypia of undetermined significance (AUS), ancillary tests like immunohistochemical (IHC) staining panels were performed. In our study, we aimed to evaluate whether the category of cases diagnosed as AUS by initial examination would change at the end of IHC studies.Materials and methods: In total, 375 serous fluid cytology samples were examined in our laboratory for 10 months. Of these, 37 cases that were initially diagnosed as AUS were included in the study. A control group, comprising 20 cases initially diagnosed as negative for malignancy (NFM) was used. For the IHC study, sections from cell blocks were used for each group Then, the slides were stained with Ep-CAM/epithelial specific antigen (MOC31), Hector Battifora mesothelial-1 (HBME-1), and cluster of differentiation 68 (CD68).Results: Following the IHC study involving MOC31, HBME-1, and CD68, a significant reclassification was observed in cases initially diagnosed as AUS. Specifically, in 86.1% of these cases, a definitive categorization into either NFM or malignant was achieved. Statistical analysis revealed a significant difference between the two groups in terms of achieving a definitive category after the IHC study (P < 0.05).Conclusion: Our study emphasizes the critical importance of enhancing the initial IHC panel, initially composed of epithelial and mesothelial markers, with CD68. This strategic addition contributed significantly to the reduction of cases categorized as AUS.","PeriodicalId":50217,"journal":{"name":"Journal of Cytology","volume":"42 1","pages":"43-47"},"PeriodicalIF":1.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11896120/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Diagnostic Study Using STARD Platform on Liquid-Based Cytology in Cervical Smear and Its Positivity Rate among Females with Abnormal Vaginal Conditions Attending a Tertiary Care Center. 使用 STARD 平台对三级医疗中心就诊的阴道异常女性进行宫颈涂片液基细胞学诊断及其阳性率研究。

IF 1 4区医学

Journal of Cytology Pub Date : 2024-10-01 Epub Date: 2024-11-08 DOI: 10.4103/joc.joc_170_23

Mohd Afzal, Abhishek Sharma, Vikas Tiwari, Jaishree Karnwal Tiwari

{"title":"A Diagnostic Study Using STARD Platform on Liquid-Based Cytology in Cervical Smear and Its Positivity Rate among Females with Abnormal Vaginal Conditions Attending a Tertiary Care Center.","authors":"Mohd Afzal, Abhishek Sharma, Vikas Tiwari, Jaishree Karnwal Tiwari","doi":"10.4103/joc.joc_170_23","DOIUrl":"10.4103/joc.joc_170_23","url":null,"abstract":"Background: Liquid-based cytology (LBC) is a newer method of preparing cervical cell samples. This technique involves collecting cells in a liquid fixative and preparing and evaluating them.Aim: This study aims to investigate cervical smears prepared using the Ezi-Prep LBC method and analyze the positivity rate for cervical cancer and assess the diagnostic accuracy of LBC in detecting cervical abnormalities among females with abnormal vaginal conditions attending a tertiary care center.Materials and methods: The present diagnostic study was performed in a tertiary care hospital over a period of 1 year (April 1, 2021-November 30, 2021). A total of 230 women, aged 21-70 years, presented at the gynecology outpatient department for either routine cervical screening or with complaints of vaginal bleeding, discharge, or pelvic pain. Cervical smears were prepared using the Ezi-Prep method and stained with ULTRA-PAP stain.Results: LBC smears are monolayer smears and are without nuclear overlapping with spread over a diameter of 16 mm. Minimum cellular overlapping and crowding were observed in LBC smears. The mean age of the participants was 35.5 ± 5.1 years. The smears were studied by using nine morphological parameters.Conclusion: The majority of the smears were inflammatory smears. A total of 16 cases and three cases were found to be bacterial vaginosis and candidiasis, respectively. In an 8-month study, HSIL was found in two cases, whereas LSIL was found in only one case. The positivity rate was observed to be 0.9% in HSIL cases and 0.4% in LSIL cases, 0.9% ASC-US case, and 0.4% invasive carcinoma case.","PeriodicalId":50217,"journal":{"name":"Journal of Cytology","volume":"41 4","pages":"207-213"},"PeriodicalIF":1.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11676091/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microscopic Papillary Thyroid Carcinoma: What It Taught Us? 显微甲状腺乳头状癌：它教给我们什么？

IF 1 4区医学

Journal of Cytology Pub Date : 2024-10-01 Epub Date: 2024-11-08 DOI: 10.4103/joc.joc_169_23

Gargi Kapatia, Mayur Parkhi, Amber Parwaiz, Archit Goel, Pranab Dey

引用次数: 0

Reed-Sternberg Cells in Pleural Effusion. 胸腔积液中的Reed-Sternberg细胞。

IF 1 4区医学

Journal of Cytology Pub Date : 2024-10-01 Epub Date: 2024-11-08 DOI: 10.4103/joc.joc_111_23

Deepika Rana, Meeta Singh, Tanvi Paul, Shramana Mandal, Puneet Kaur Shahi

引用次数: 0

A Combination of Cell Findings Improves the Accuracy of Voided Urine Cytology. 多种细胞检查结果的结合提高了空尿细胞学检查的准确性。

IF 1 4区医学

Journal of Cytology Pub Date : 2024-10-01 Epub Date: 2024-11-08 DOI: 10.4103/joc.joc_10_24

Sachiko Iwai, Mitsuru Kinjo

{"title":"A Combination of Cell Findings Improves the Accuracy of Voided Urine Cytology.","authors":"Sachiko Iwai, Mitsuru Kinjo","doi":"10.4103/joc.joc_10_24","DOIUrl":"10.4103/joc.joc_10_24","url":null,"abstract":"Introduction: Urine cytology is a morphological diagnostic test that is, patient-friendly and easy to sample but subjective in morphological evaluation. This study aims to evaluate the effect of combining cell findings to assess urine cytology.Materials and methods: Thirty cell findings found in high-grade urothelial carcinoma (HGUC) were selected for morphological abnormalities, each with detailed definitions. All 66 HGUC and 30 benign cases were examined for morphological abnormalities. The number of cases with at least one abnormal cell finding deviating from normal cells was used. The HGUC and benign cases were compared using significance difference tests. Using the cell findings extracted by the exact tests, discriminant analysis was performed to determine the valid cell findings for HGUC diagnosis.Results: There was a difference in the detection rate of abnormal findings between HGUC and benign cases. Additionally, 20 cell findings were detected significantly more frequently in patients with HGUC. Furthermore, the discriminant analysis revealed that six cellular findings (high nucleus-to-cytoplasm ratio, hyperchromasia, eccentric nuclei, nuclear protrusion, unevenly distributed chromatin, and irregular nuclear shape) showed high accuracy in confirming the diagnosis of HGUC.Conclusions: Five- to six-cell findings were individually valid; however, the combination of cell findings is crucial for an objective and accurate diagnosis of HGUC.","PeriodicalId":50217,"journal":{"name":"Journal of Cytology","volume":"41 4","pages":"183-191"},"PeriodicalIF":1.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11676093/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fine Needle Aspiration Cytology of Ovarian Tumors With Histopathological Correlation: A Revisit to Borderline Category. 卵巢肿瘤的细针抽吸细胞学与组织病理学相关性：重新审视边缘类别。

IF 1 4区医学

Journal of Cytology Pub Date : 2024-10-01 Epub Date: 2024-11-08 DOI: 10.4103/joc.joc_196_23

Zoya Hasan, Meeta Singh, Jyoti Singh, Gautam Chellani, Irfana Nisam, Garima Rakheja, Shyama Jain, Nita Khurana, Shramana Mandal, Arun K Rathi, Yedla M Mala, Radhika Batra

{"title":"Fine Needle Aspiration Cytology of Ovarian Tumors With Histopathological Correlation: A Revisit to Borderline Category.","authors":"Zoya Hasan, Meeta Singh, Jyoti Singh, Gautam Chellani, Irfana Nisam, Garima Rakheja, Shyama Jain, Nita Khurana, Shramana Mandal, Arun K Rathi, Yedla M Mala, Radhika Batra","doi":"10.4103/joc.joc_196_23","DOIUrl":"10.4103/joc.joc_196_23","url":null,"abstract":"Background: Borderline ovarian tumors (BOTs) comprise 15%-20% of all ovarian epithelial malignancies. The majority of them are serous tumors followed by mucinous tumors. Pre-operative cytological diagnosis plays an important role with histopathology being the gold standard. The present study aimed to assess the sensitivity, specificity, and diagnostic accuracy of cytology in the diagnosis of epithelial ovarian tumors with special emphasis on the cytologically borderline category.Materials and methods: The present study was conducted in our institute over five years from January 2017 to December 2022. This was a retrospective study and included 92 patients with ovarian masses who were subjected to ultrasound-guided fine needle aspiration (FNA) as a preliminary diagnostic procedure. Cytology smears were examined and categorized as benign, borderline, and malignant. The sensitivity, specificity, and diagnostic accuracy were assessed considering histopathology as the gold standard.Results: In the present study, 92 ovarian FNA cytology were performed. Aspirates were adequate in all but 10 cases. Out of 82 cases, 75 were epithelial tumors. Twelve cases of BOTs were compiled and cytological findings were assessed.Conclusion: To conclude, diagnosis of BOTs on cytology is difficult, however, aspiration from multiple sites from the lesion, critical cytomorphological assessment, and cell-block preparation with immunocytochemistry/immunohistochemistry can overcome the pitfalls.","PeriodicalId":50217,"journal":{"name":"Journal of Cytology","volume":"41 4","pages":"229-235"},"PeriodicalIF":1.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11676087/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0