Journal of Cytology最新文献

{"title":"Role of FNAC in Identifying the Mysterious Mite","authors":"Premalatha Balakrishnan, K. Dhanalakshmi, V. Ramesh, M. Dharani","doi":"10.4103/joc.joc_2_23","DOIUrl":"https://doi.org/10.4103/joc.joc_2_23","url":null,"abstract":"","PeriodicalId":50217,"journal":{"name":"Journal of Cytology","volume":"21 9","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139150188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liquid-Based Cervical Cytology: Monitoring the Laboratory Quality Indicators","authors":"Sonali Susmita Nayak, Anupurva Dutta, Rutvij Khedkar, Sabyasachi Roy","doi":"10.4103/joc.joc_112_23","DOIUrl":"https://doi.org/10.4103/joc.joc_112_23","url":null,"abstract":"Many developments in cervical cancer screening have happened in the past century, helping women in earlier detection of cervical cancer and its precursors. Cytology still holds the fort as being a specific test, though it suffers in sensitivity. As a part of the quality control program, the aim of the study is to determine the total number of abnormal liquid-based cervical cytology (LBC) at our center and correlate the abnormal LBC with histology and human papillomavirus (HPV) DNA test results. Retrospective analysis of 4286 LBC screening cases was carried out over a period of 5 years. For cytology–histology correlation, cervical biopsy and cytology test results were analyzed. The two-tier grading system for biopsy interpretation was used. HPV DNA test results wherever available were correlated. Of the 4286 LBC cases, 157 samples (3.7%) were unsatisfactory for evaluation, 3915 samples (91.3%) were negative for intra-epithelial lesion or malignancy, and 214 samples (5%) showed epithelial cell abnormality. ASC-US was reported in 60 cases (1.4%), ASC-H in 35 cases (0.8%), LSIL in 47 cases (1.1%), HSIL in 41 cases (1.0%), squamous cell carcinoma in a single case (0.02%), and atypical glandular cells in 30 cases (0.7%). The ASC/SIL ratio was 1.07:1. The CHC major discrepancy was calculated as 16.2%. The concordance of HSIL on cytology and biopsy as a measure of PPV is 94.4%. Of the epithelial cell abnormalities, 24 cases were positive for high-risk HPV (hrHPV). Molecular test results of 2737 samples showed HPV detected in 50 cases, of which 24 cases were positive for hrHPV. The study helped us to analyze the quality parameters of our cytopathology laboratory which are within the acceptable limits.","PeriodicalId":50217,"journal":{"name":"Journal of Cytology","volume":"56 8","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139149450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gross Appearance of Fine Needle Aspiration Smears: Can Adequacy of the Sample be Assessed and Correlated with their Microscopic Cytological Yield","authors":"Sushma Ramraje, Prabal Deb, B. N. Hiwale, B. Deb","doi":"10.4103/joc.joc_149_23","DOIUrl":"https://doi.org/10.4103/joc.joc_149_23","url":null,"abstract":"Fine needle aspiration cytology (FNAC) is a simple procedure that is widely accepted as a first-line investigation. It should ideally be performed near the patient with microscopic assessment for evaluation of adequacy of aspirate. Since this is not always possible, a worthwhile substitute is to assess the gross appearance of the material obtained. This study was aimed to determine the value of this alternative rapid, bed-side approach. This study was carried out in a tertiary care hospital for a duration of 1 year, where 50 cases undergoing FNAC in the out-patient department (OPD) were included. Unstained smears of commonly encountered lesions were photographed and assessed by three independent observers, who graded them into four grades, viz. Grade 1: unlikely to contain diagnostic material. Grade 2: possibly contains diagnostic material. Grade 3: probably contains diagnostic material. Grade 4: material suggesting a specific diagnosis. Results were subsequently compared with the microscopic findings of stained smears. Distribution of the 50 cases between grades 1, 2, 3, and 4 were 5, 6, 17, and 22, respectively. Gross appearances of FNA smears are helpful in predicting the adequacy of the sample and sometimes the final microscopic diagnosis. The cellularity of a sample can be gauged by inspection and sometimes a likely diagnosis can be made. Some lesions show typical gross appearance easily detected by the experienced eye so a likely diagnosis can be predicted prior to microscopic examination. However, near-patient microscopic assessment of FNA specimens for rapid onsite evaluation, if available, should be the preferred mode.","PeriodicalId":50217,"journal":{"name":"Journal of Cytology","volume":"348 6","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139152300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral Paper Abstract","authors":"","doi":"10.4103/0970-9371.388618","DOIUrl":"https://doi.org/10.4103/0970-9371.388618","url":null,"abstract":"","PeriodicalId":50217,"journal":{"name":"Journal of Cytology","volume":"296 1 1","pages":"5 - 35"},"PeriodicalIF":1.3,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139328581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Award Abstract","authors":"","doi":"10.4103/0970-9371.388611","DOIUrl":"https://doi.org/10.4103/0970-9371.388611","url":null,"abstract":"","PeriodicalId":50217,"journal":{"name":"Journal of Cytology","volume":"41 1","pages":"1 - 4"},"PeriodicalIF":1.3,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139325234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Structural Profile of HPV 18 in Terms of Chromosomal and Nuclear Degenerative Changes and the Ratio of Nucleus/Cytoplasm on Liquid based Cervical Cells.","authors":"Zehra Safi Oz,&nbsp;Figen Barut,&nbsp;Furuzan Kokturk,&nbsp;Banu Dogan Gun","doi":"10.4103/joc.joc_72_22","DOIUrl":"10.4103/joc.joc_72_22","url":null,"abstract":"<p><strong>Background: </strong>HPV 18 is one of the important oncogenic types. HPV 18 is generally evaluated together with HPV 16 and/or high-risk HPV types in light microscopic studies. The purpose of this study was to evaluate the impact of only HPV 18 on the nucleus/cytoplasm ratio, and chromosomal and nuclear degenerative changes in liquid-based samples.</p><p><strong>Materials and methods: </strong>Eighty liquid-based cervical samples were used in this retrospective study. These smears were prepared by HPV Deoxyribonucleic Acid (DNA) detection and genotyping with the Cobas 4800 HPV system. Forty HPV 18 infected and forty smears with no infection agent were evaluated for chromosomal (nuclear budding, micronuclei), nuclear degenerative changes (membrane irregularity, nuclear enlargement, hyperchromasia, abnormal chromatin distribution, binucleation (BN), karyorrhexis (KR), karyolysis (KL), karyopyknosis (KP)), and cytologic findings (koilocyte (KC), cells with perinuclear PR) using light microscopy. Cellular diameters were evaluated using image analysis software. Statistical analysis was performed with Statistical Package for Social Sciences (SPSS) 19.0. <i>p</i> values < .05 were considered significant.</p><p><strong>Results: </strong>The statistically significant difference between the presence of HPV 18 and karyorrectic cell, KC, nuclear membrane irregularity, enlargement, the mean nuclear width and height (<i>p</i> < 0.05). No cellular changes other than those mentioned were observed.</p><p><strong>Conclusions: </strong>The present study is significant in that, it reveals the relationship between only and particularly HPV 18 and nucleus/cytoplasm ratio, and chromosomal and nuclear degenerative changes in liquid-based cytology. HPV 18 affects KR, koilocytosis, nuclear membrane irregularity, enlargement, and nuclear diameters. Light microscopic analysis of these abnormalities increases the sensitivity and specificity of cytology in the evaluation of cellular pictures due to HPV 18.</p>","PeriodicalId":50217,"journal":{"name":"Journal of Cytology","volume":"40 3","pages":"133-139"},"PeriodicalIF":1.3,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10516153/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41137688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of Novel Tiered Reporting System in Serous Fluid Cytology and Risk of Malignancy Assessment: A Retrospective Study in a Tertiary Care Center.","authors":"Soumya Alashetty,&nbsp;Balu Sadasivan,&nbsp;Priya Dharmalingam,&nbsp;Nethra Rajagopal,&nbsp;Lucas Kavya,&nbsp;Malathi M Pai","doi":"10.4103/joc.joc_107_22","DOIUrl":"10.4103/joc.joc_107_22","url":null,"abstract":"<p><strong>Background: </strong>Serous effusion cytology (SEC) reporting is important for the management of the cancer patient. The International System for Reporting Serous Fluid Cytology (ISRSFC) provides tiered reporting terminology to standardize practice, looking into the risk of malignancy (MAL) for each category. In this study, we have assessed the utility of the ISRSFC and reported our experience at a tertiary cancer center.</p><p><strong>Materials and methods: </strong>Serous fluid cytology reported from January 2019 to December 2020 was categorized according to ISRSFC diagnostic categories: nondiagnostic (ND), negative for MAL (NFM), atypia of undetermined significance (AUS), suspicious for MAL (SFM), and MAL. The risk of MAL (ROM) and performance parameters were calculated.</p><p><strong>Results: </strong>A total of 2150 serous effusions including 1160 pleural, 929 peritoneal, and 61 pericardial effusions from 2071 patients were reported. There were 742 males and 1329 females. The patient's age ranged from less than 1 yr to 95 years. The volume of the sample ranged from 0.5 ml to 2000 ml. There were 114 ND (5.32%), 1068 NFM (49.67%), 144 AUS (6.69%), 82 SFM (3.81%), and 742 MAL (34.51%) cases. Adenocarcinoma was the most common MAL involving serous fluids (91.50%). The calculated ROM was 15.38% for ND, 24.26% for NFM, 62.96% for AUS, 79.16% for SFM, and 100% for MAL. Sensitivity, specificity, positive predictive value, negative predictive value, and diagnostic accuracy were, respectively, 68.04%, 96.96%, 96.35%, 72.07%, and 81.33%.</p><p><strong>Conclusion: </strong>ISRSFC provides consistent reporting terminology and the ROM for each category aids in clinical correlation and management.</p>","PeriodicalId":50217,"journal":{"name":"Journal of Cytology","volume":"40 3","pages":"107-113"},"PeriodicalIF":1.3,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10516159/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41138867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heat Induced Processing of Cellblocks with Significant Reduction in Overall Turn Around Time.","authors":"Suhas Dhende,&nbsp;Saleem Pathuthara,&nbsp;Neelam Prabhudesai,&nbsp;Dipak Shinde,&nbsp;Nupur Karnik,&nbsp;Kedar Deodhar","doi":"10.4103/joc.joc_34_23","DOIUrl":"10.4103/joc.joc_34_23","url":null,"abstract":"<p><strong>Introduction: </strong>Cellblock (CB) with immunohistochemistry (IHC) is practically indispensable in the diagnostic workup of serous effusions; however, CB requires a minimum of 15-20 h for routine histopathological processing. A reduction in processing time can expedite a faster diagnosis.</p><p><strong>Aim: </strong>This study was undertaken to evaluate the utility of the heat-induced CB (HICB) technique.</p><p><strong>Material and methods: </strong>Two sets of agar-embedded CBs were processed from 50 effusion samples. CBs were further processed by conventional and rapid methods. Conventional CBs (CCB) were processed in a histoprocessor, whereas rapid CB was processed in a heated water bath with an agitation facility. For HICB processing, dehydration and clearing were performed at 50°C followed by paraffin wax impregnation at 65°C temperature. From both CBs, sections of 5 um thickness were cut and stained with hematoxylin and eosin (H and E). Cell morphology, cost, and time were compared between the two methods. The feasibility of IHC was attempted in a few cases.</p><p><strong>Results: </strong>HICB was completed within 4.30 h compared with CCB. Diagnoses on both CBs were concordant in all the cases. Incomplete dehydration was noted in six (12%) cases, but the diagnosis was not compromised. No additional cost was involved in HICB. On IHC, both HICB and CCB exhibited equivalent expression.</p><p><strong>Conclusions: </strong>HICB is a rapid, innovative, simple, and cost-effective technique and expedites faster diagnosis. It does not require any advanced equipment.</p>","PeriodicalId":50217,"journal":{"name":"Journal of Cytology","volume":"40 3","pages":"126-132"},"PeriodicalIF":1.3,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10516157/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41151361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Similarities and Differences between Liquid-based and Conventional Methods in Evaluation of Thyroid Fine Needle Aspiration Cytologies.","authors":"Mehmet Özer,&nbsp;Selver Özekinci","doi":"10.4103/joc.joc_27_23","DOIUrl":"10.4103/joc.joc_27_23","url":null,"abstract":"<p><strong>Aims: </strong>Thyroid nodules are one of the most frequent medical issues in endocrinology in our country and around the world. The appropriate evaluation of the nodule is critical in the management of patients. Fine needle aspiration cytology (FNAC) is one of the most accurate tools for evaluating these nodules. Conventional and liquid-based (LB) methods are available for thyroid FNAC. In this paper, we aim to determine the best cytological method for the evaluation of thyroid fine needle aspiration materials.</p><p><strong>Settings and design: </strong>Fine needle aspiration (FNA) materials obtained and prepared by conventional and LB methods from 310 patients were evaluated. These slides were compared in terms of 12 cytological parameters in a three-tiered system, and the kappa coefficient was calculated.</p><p><strong>Methods and material: </strong>Two slides were prepared from FNA samples, and the rest of the material was left in protective solution for LB cytology. Surepath was used as a LB technique. Conventional slides (CS) were left to dry in the air and stained with May Grunwald Giemsa. Slides prepared by two different techniques were compared in 12 cytomorphological parameters, and Kappa coefficients and correlations were calculated.</p><p><strong>Statistical analysis used: </strong>Kappa statistics.</p><p><strong>Results: </strong>In comparison of LB and CS, the highest kappa coefficient was in chromatin texture (ĸ:0.738) and inflammatory cells (ĸ: 0.482). On other parameters, fair or poor agreement was observed.</p><p><strong>Conclusions: </strong>Although there are some superiorities to LB cytology, co-application of these two methods is more favorable in thyroid fine needle aspiration.</p>","PeriodicalId":50217,"journal":{"name":"Journal of Cytology","volume":"40 3","pages":"140-144"},"PeriodicalIF":1.3,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10516154/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41174297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytodiagnosis of Clival Chordoma.","authors":"Ranjana Giri,&nbsp;Anjali Agarwal,&nbsp;Prajna Dash,&nbsp;Subrat K Sahu,&nbsp;Kabikanta K Samantray","doi":"10.4103/joc.joc_145_22","DOIUrl":"10.4103/joc.joc_145_22","url":null,"abstract":"","PeriodicalId":50217,"journal":{"name":"Journal of Cytology","volume":"40 3","pages":"153-154"},"PeriodicalIF":1.3,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10516158/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41150389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}