Journal of Infectious Diseases最新文献

{"title":"Characterization of c-Src during Listeria monocytogenes cell-to-cell spreading.","authors":"Petra McLeod, Aaron S Dhanda, Julian A Guttman","doi":"10.1093/infdis/jiaf063","DOIUrl":"https://doi.org/10.1093/infdis/jiaf063","url":null,"abstract":"<p><p>Listeria monocytogenes replicates within host cells and spreads from cell-to-cell using actin-based motility. Cell-to-cell movement of L. monocytogenes is achieved by creating actin-rich membrane protrusions (listeriopods), which generate corresponding invaginations in adjacent cells through caveolin-mediated endocytosis. We show that c-Src, a multifunctional tyrosine kinase, is enriched at invaginations and is crucial for efficient cell-to-cell spreading of the bacteria as cells expressing c-Src mutants that were either constitutively active or those that impeded the function of c-Src resulted in significantly more (or less) cell-to-cell spreading. This work demonstrates the importance of c-Src in influencing L. monocytogenes' ability to spread intercellularly.</p>","PeriodicalId":50179,"journal":{"name":"Journal of Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143081908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization of RSV infections in elderly patients during the 2023/2024 season in the era of nirsevimab introduction.","authors":"Amandine Caillault, Laurent Softic, Pierre Bay, Arnaud Ly, Alexandre Soulier, Giovanna Melica, Christophe Rodriguez, Nicolas de Prost, Jean-Michel Pawlotsky, Slim Fourati","doi":"10.1093/infdis/jiaf062","DOIUrl":"https://doi.org/10.1093/infdis/jiaf062","url":null,"abstract":"<p><p>RSV can cause severe infections in the elderly. Nirsevimab is a novel prophylactic monoclonal antibody, widely used in infants in France during the 2023/2024 season. It may select for resistant RSV variants that, if transmitted in the community, could compromise vaccine efficacy in the elderly. In this study, we analyzed RSV full-length genomes (68% RSV-A, 32% RSV-B) from 125 patients >60y during 2023/2024 season. Genetic diversity of RSV-F was low, with no resistance-associated substitutions (RASs) detected. While no RASs were identified, ongoing monitoring is essential to prevent the emergence of resistant variants that could affect vaccine efficacy in the elderly.</p>","PeriodicalId":50179,"journal":{"name":"Journal of Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143081913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"10 years of Severe Acute Respiratory Infections Network (SARInet plus): accomplishments and way forward.","authors":"Marc Rondy, Eduardo Azziz-Baumgartner, Rebecca Kondor, Rakhee S Palekar, Wenqing Zhang, Andrea S Vicari","doi":"10.1093/infdis/jiaf039","DOIUrl":"https://doi.org/10.1093/infdis/jiaf039","url":null,"abstract":"","PeriodicalId":50179,"journal":{"name":"Journal of Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pandemic preparedness: analyzing national plans for respiratory pathogen pandemics in the Americas region.","authors":"Andrea Patricia Villalobos, Iyanna Wellington, Shoa Moosavi, Ana de la Garza, Ángel Rodríguez, Britney McMurren, Juliana Almeida Leite, Jairo Andrés Méndez-Rico, Lidia Redondo, Miriam Esther Blanco, Olaya Astudillo, Paula Couto, Priscila Born, Sara Hess, Shoshanna Goldin, Hannah Lewis, Tamara Mancero, Andrea Vicari, Marc Rondy","doi":"10.1093/infdis/jiaf047","DOIUrl":"https://doi.org/10.1093/infdis/jiaf047","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;The International Health Regulations (2005) (IHR), requires that States Parties develop their capacities to detect, assess, and respond to public health threats and report to the World Health Assembly through the States Parties Annual Report (SPAR). The National Pandemic Preparedness and Response Plans (PPRP) contribute to countries capacities however there are some discrepancies between both tools. To identify gaps and define priority actions to strengthen pandemic plans, we assessed the concordance between national pandemic preparedness and response plans for respiratory pathogens against the pandemic checklist published in 2023 and the SPAR.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;In this retrospective, semi-quantitative study, conducted in August 2024, we reviewed the most recent respiratory pandemic plans for 35 PAHO member states and assessed their concordance with (1) actionable guidelines in the World Health Organization pandemic checklist and (2) IHR (2005) core capacities using the latest SPAR tool. We developed 25 tracking questions to identify gaps, strengths, and opportunities for improvement in the pandemic plans, using the pandemic checklist built on the capacities and capabilities described in the WHO's Preparedness and Resilience for Emerging Threats (PRET) Module 1. We used a five-point scale (from 1, when the subcomponent was not mentioned, to 5, when the subcomponent was described at all levels), and we calculated the average pandemic plans score (PP score) for each component. Data from pandemic plans (2005-2024) were compiled, selected, analyzed, and scored. We compared the average SPAR score and the PP score to assess areas of convergence and variance between preparedness and capacities. The analysis was carried out using R and Excel.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;We analyzed 35 respiratory pandemic plans: 29 were influenza-specific, five were COVID-19-specific, and one was not pathogen-specific. Most current national plans showed limited alignment with the content recommended in the PRET pandemic checklist. At regional level, the lowest concordance between plans and pandemic checklist was in the following subcomponents Public Health and Social Measures (80% of the plans had a score of 1); Emergency, Logistics and Supply Chain Management (74%); and Research and Development (71%). Conversely, the strongest subcomponents (≥40% of plans with a score of 4 or 5) were: Policy, Legal, and Normative Instruments (45%); Coordination (46%); and Surveillance: early detection and assessment (43%). In most countries, the SPAR scores tended to be higher than PP scores, except for Argentina (the newest plan reviewed) for which the pattern was reversed, and the PP scores exceeded the SPAR scores.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;Given the gaps identified between current plans and the global standards espoused by the PRET Module 1 initiative, it is recommended that countries build on the strengths of their national","PeriodicalId":50179,"journal":{"name":"Journal of Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strengthening the Surveillance and Response to Public Health Events with a One Health approach: a perspective from 12 countries in Latin America and the Caribbean.","authors":"Angel Rodriguez, Paula Couto, Alejandra Acevedo, Belsy Acosta Herrera, Olaya Astudillo, Martín Avaro, Gisela Barrera Badillo, Alfredo Bruno Caicedo, Patricia Bustos, Mauricio Cerpa, Hector Chiparelli, Dulce Curon, Rodrigo Fasce, Axel Galo, Elías Guilarte, Irma López Martínez, Mariela G Martínez, Jairo Mendez-Rico, María Fernanda Olivares Barraza, Elena Penayo, María Paz Rojas Mena, Paula Rodríguez Ferrari, Marc Rondy, Viviana Sotomayor Proschle, Priscila S Born, Lidia Redondo, Juliana A Leite, Natalia Vergara Mallegas, Marta von Horoch, Andrea Patricia Villalobos, Andrea Vicari","doi":"10.1093/infdis/jiae629","DOIUrl":"https://doi.org/10.1093/infdis/jiae629","url":null,"abstract":"","PeriodicalId":50179,"journal":{"name":"Journal of Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From Epidemics to Pandemics: Over a Decade of SARInet Strengthening and Laboratory Surveillance and Response for Respiratory Viruses in the Americas.","authors":"Juliana A Leite, Priscila S Born, Angel Rodriguez, Paula Couto, Mauricio Cerpa, Jean-Marc Gabastou, Lionel Gresh, Leticia Franco, Mariela G Martínez, Madelaine Sugasti, Jorge Jara, Lidia Redondo Bravo, Iyanna W Perkins, Andrea Patricia Villalobos, Rosa Ramirez, Ximena Terrazas, Juliana Barbosa, Marc Rondy, Andrea Vicari, Sylvain Aldighieri, Richard Webby, Wenqing Zhang, Dimitry Pereyaslov, Magdi Samaan, Jairo Mendez-Rico","doi":"10.1093/infdis/jiaf046","DOIUrl":"https://doi.org/10.1093/infdis/jiaf046","url":null,"abstract":"","PeriodicalId":50179,"journal":{"name":"Journal of Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Review: Current Laboratory and Point-of-Care Pharyngitis Diagnostic Testing and Knowledge Gaps.","authors":"","doi":"10.1093/infdis/jiaf024","DOIUrl":"https://doi.org/10.1093/infdis/jiaf024","url":null,"abstract":"","PeriodicalId":50179,"journal":{"name":"Journal of Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143069307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resistance Analyses in Heavily Treatment-Experienced People with HIV Treated with the Novel HIV Capsid Inhibitor Lenacapavir After 2 years.","authors":"Nicolas A Margot, Vamshi Jogiraju, Nina Pennetzdorfer, Vidula Naik, Laurie A VanderVeen, John Ling, Renu Singh, Hadas Dvory-Sobol, Onyema Ogbuagu, Sorana Segal-Maurer, Jean-Michel Molina, Martin S Rhee, Christian Callebaut","doi":"10.1093/infdis/jiaf050","DOIUrl":"https://doi.org/10.1093/infdis/jiaf050","url":null,"abstract":"<p><strong>Background: </strong>Lenacapavir is a highly potent first-in-class inhibitor of HIV-1 capsid approved for the treatment of heavily treatment-experienced (HTE) people with HIV-1 (PWH) harboring multidrug resistant (MDR) virus, in combination with an optimized background regimen (OBR). Resistance analyses conducted after 2 years of lenacapavir treatment in the phase 2/3 CAPELLA study are described.</p><p><strong>Methods: </strong>CAPELLA enrolled viremic HTE PWH with resistance to 2 or more drugs per class in at least 3 of the 4 main drug classes. Post-baseline resistance was evaluated in participants experiencing virologic failure using resistance assays (HIV-1 capsid, protease, reverse transcriptase, and integrase genotypic/phenotypic tests). Adherence to OBR was assessed by plasma drug measurement using tandem liquid chromatography/mass spectrometry.</p><p><strong>Results: </strong>After 2 years, lenacapavir plus OBR treatment led to HIV-1 RNA suppression in 82% of participants (missing=excluded). Treatment-emergent capsid resistance occurred in 19% (14/72) of participants, including capsid mutations M66I, Q67H/K/N, K70H/N/R/S, and/or N74D/H/K, which were all associated with functional lenacapavir monotherapy. Seven participants with lenacapavir resistance reattained HIV-1 RNA <50 copies/mL upon OBR resumption or change, while remaining on lenacapavir.</p><p><strong>Conclusions: </strong>Emergence of lenacapavir resistance after 2 years in CAPELLA was a consequence of functional lenacapavir monotherapy. In half of participants with lenacapavir resistance, continued treatment with lenacapavir + active OBR led to HIV-1 RNA resuppression.</p>","PeriodicalId":50179,"journal":{"name":"Journal of Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143054075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global Impact of Mass Vaccination Campaigns on Circulating Type 2 Vaccine-Derived Poliovirus Outbreaks: An Interrupted Time-Series Analysis.","authors":"Laura V Cooper, Ananda S Bandyopadhyay, Nicholas C Grassly, Elizabeth J Gray, Arie Voorman, Simona Zipursky, Isobel M Blake","doi":"10.1093/infdis/jiae614","DOIUrl":"https://doi.org/10.1093/infdis/jiae614","url":null,"abstract":"<p><strong>Background: </strong>Between 2016 and 2023, 3248 cases of circulating vaccine-derived type 2 poliomyelitis (cVDPV2) were reported globally and supplementary immunization activities (SIAs) with monovalent type 2 oral poliovirus vaccine (mOPV2) and novel type 2 oral poliovirus vaccine (nOPV2) targeted an estimated 356 and 525 million children, respectively. This analysis estimates the community-level impact of nOPV2 relative to mOPV2 SIAs.</p><p><strong>Methods: </strong>We fitted interrupted time-series regressions to surveillance data between January 2016 and November 2023 to estimate the impact of nOPV2 and mOPV2 SIAs on cVDPV2 poliomyelitis incidence and prevalence in environmental surveillance across 37 countries, directly comparing the impact of SIAs in 13 countries where both vaccines were used.</p><p><strong>Results: </strong>We did not find any statistically significant differences between nOPV2 and mOPV2 SIA impact except for in the Democratic Republic of Congo (DRC), where nOPV2 SIAs had lower impact (adjusted relative risk [aRR] for cVDPV2 poliomyelitis incidence per nOPV2 SIA, 0.505; 95% confidence interval [CI], .409-.623) compared to mOPV2 (aRR, 0.193; 95% CI, .137-.272); P value for difference in RRs = 3e-6.</p><p><strong>Conclusions: </strong>We find variation in OPV2 SIA impacts globally, with greater certainty about Nigeria and DRC, where large outbreaks provided an opportunity to assess impact at scale. In most countries, we find no significant difference between nOPV2 and mOPV2 SIA impact. We are unable to identify the reason for the significant difference in DRC, which could include differential SIA coverage, timing, vaccine effectiveness, or outbreak dynamics.</p>","PeriodicalId":50179,"journal":{"name":"Journal of Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143054072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associations between the gut microbiome, inflammation and cardiovascular profiles in people with HIV.","authors":"Rachel MacCann, Junhui Li, Alejandro Abner Garcia Leon, Riya Negi, Dana Alalwan, Willard Tinago, Padraig McGettrick, Aoife G Cotter, Alan Landay, Caroline Sabin, Paul W O'Toole, Patrick W Mallon","doi":"10.1093/infdis/jiaf043","DOIUrl":"https://doi.org/10.1093/infdis/jiaf043","url":null,"abstract":"<p><strong>Background: </strong>Inflammation and innate immune activation are associated with chronic HIV infection, despite effective treatment. Although gut microbiota alterations are linked to systemic inflammation, the relationships between the gut microbiome, inflammation and HIV remain unclear.</p><p><strong>Methods: </strong>The UPBEAT-CAD sub-study, examining cardiovascular disease (CVD) risk in HIV, enrolled participants matched on HIV status and traditional CVD risk factors. Subclinical CVD was assessed using coronary computed tomography angiography (CCTA). 34 biomarkers were measured using quantitative immunoassays. Microbiota composition was analysed by 16S rRNA sequencing of stool samples, with taxonomic assignment via the SPINGO pipeline. Differentially abundant species were identified by Analysis of Compositions of Microbiomes with Bias Correction (ANCOM-BC) and correlated to biomarkers, diet and CCTA outcomes using Spearman correlation.</p><p><strong>Results: </strong>Among 81 participants (median age 51 years, 73% male), people with HIV (n=44 , 54%) had a higher prevalence of hypercholesterolaemia (p <0.025) and statin use (p <0.001). A significant separation in gut microbiome β-diversity was observed between people with and without HIV. ANCOM-BC analysis identified 42 differentially abundant species and 10 genera in those with HIV. Enrichment of Bifidobacterium pseudocatenulatum, Megamonas hypermegale and Selenomonas ruminantium and depletion of Fusicatenenibacter correlated with lower plaque burden. Depletion of SCFA-producing Ruminococcus bromii correlated with higher plaque burden and fat intake, while depletion of Bacteroides spp and Alistepes spp correlated with elevated inflammatory biomarkers (D-dimer, CD40-ligand, CRP and IFN-γ).</p><p><strong>Conclusion: </strong>Significant gut microbiota differences in people with HIV were linked to subclinical CVD, diet, and inflammation, suggesting a role for the microbiome in cardiovascular risk in HIV infection.</p>","PeriodicalId":50179,"journal":{"name":"Journal of Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143034024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}