Transcriptome Analysis of Monocytes Treated with Dengue NS1 Revealed a Shift in Transcripts Involving in Self-Propagated Proinflammation and Antiviral Responses.
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引用次数: 0
Abstract
Non-structural protein 1 (NS1) of dengue virus (DENV) can influence dengue severity. In this study, we used RNA sequencing analysis to assess blood monocyte response to different concentrations of NS1. Here we showed that NS1 at the level found in severe dengue may be involved in severe dengue development through two potential mechanisms which were induction of excessive inflammation and suppression of antiviral responses. At high levels, NS1 significantly up-regulated S100A8 and S100A9, ACOD1, and TREM-1, that might help amplifying the inflammatory loops. In terms of antiviral suppression, we found that high NS1 concentration significantly suppressed interferon signalling and MHCII transcripts. This potentially delayed the clearance of both DENV and NS1 protein. Our study highlighted the possible role of NS1-activated monocytes in dengue severity.
期刊介绍：
Published continuously since 1904, The Journal of Infectious Diseases (JID) is the premier global journal for original research on infectious diseases. The editors welcome Major Articles and Brief Reports describing research results on microbiology, immunology, epidemiology, and related disciplines, on the pathogenesis, diagnosis, and treatment of infectious diseases; on the microbes that cause them; and on disorders of host immune responses. JID is an official publication of the Infectious Diseases Society of America.
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