{"title":"Transcriptional regulation of GmNAC3-mediated drought stress tolerance in soybean.","authors":"Nooral Amin,Liu Lu,Faizur Rehman,Muhammad Imran,Gai Yuhong,Piwu Wang,Wei Jian","doi":"10.1080/21645698.2025.2516295","DOIUrl":"https://doi.org/10.1080/21645698.2025.2516295","url":null,"abstract":"Drought stress is a major limiting factor that adversely affects both the yield and quality of soybean crops. Transcription factors (TFs) play a pivotal role in regulating gene expression, facilitating plant adaptation and response to various abiotic stresses. Among the 179 NAC TFs encoded in the soybean genome, several are differentially expressed under stress conditions; however, the functional role of GmNAC3 in drought tolerance remains largely unknown. In this study, we cloned the 840 bp coding sequence of GmNAC3 and developed transgenic soybean hairy roots via Agrobacterium-mediated transformation to explore its role in drought response. The physiological and molecular responses of GmNAC3 overexpression (OE) chimeric soybean plants were assessed under polyethylene glycol (PEG)-simulated drought stress using Hoagland nutrient solution. Compared to empty vector (EV) controls, OE plants exhibited enhanced drought tolerance, including improved phenotypic traits, better root development, and stress resilience. Notably, OE plants showed a 23.9% reduction in hydrogen peroxide accumulation and a 31.25% decrease in superoxide anion levels. Biomass analysis on MS medium revealed significantly higher fresh and dry weights of OE hairy roots across different mannitol concentrations compared to EV roots. Furthermore, GmNAC3 overexpression led to the upregulation of key downstream genes involved in stress response, particularly GmLAC5 and GmLAC7. These findings suggest that GmNAC3 enhances drought tolerance in soybean by regulating both physiological and molecular pathways. Overall, GmNAC3 represents a promising target for genetic engineering aimed at improving drought resistance in soybean and potentially other crops.","PeriodicalId":501763,"journal":{"name":"GM Crops & Food","volume":"16 1","pages":"435-449"},"PeriodicalIF":0.0,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144370193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
GM Crops & FoodPub Date : 2025-06-20DOI: 10.1080/21645698.2025.2520664
Tianheng Mou,Qiuchi Song,Yonghong Liu,Jun Song
{"title":"Initiating the commercialization of genetically modified staple crops in China: domestic biotechnological advancements, regulatory milestones, and governance frameworks.","authors":"Tianheng Mou,Qiuchi Song,Yonghong Liu,Jun Song","doi":"10.1080/21645698.2025.2520664","DOIUrl":"https://doi.org/10.1080/21645698.2025.2520664","url":null,"abstract":"Historically, China's approach to genetically modified (GM) staple crops has been cautious. However, in 2021, China launched its first pilot program for the commercial cultivation of GM food crops and subsequently expanded their cultivation. Previously, the only GM plants cultivated in China were insect-resistant cotton and virus-resistant papaya. The regulatory and policy shifts, from initial research and cautious development to accelerated commercialization, led to significant changes in China's safety-related regulatory framework for genetically modified organism (GMOs). Here, China's progress in GMO research and commercialization over the past three decades is comprehensively analyzed. This review traces the evolution of core regulations governing GMO safety, summarizes the Chinese model of GMO safety governance, and highlights the remaining challenges as commercialization progresses. The goal was to present the international community with a GMO safety governance model reflecting China's characteristics and practices, offering a Chinese solution to balancing GMO commercial adoption with ecological preservation.","PeriodicalId":501763,"journal":{"name":"GM Crops & Food","volume":"51 1","pages":"450-481"},"PeriodicalIF":0.0,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144328857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The invasion of fall armyworm and green pest control: an analysis of farmers' willingness to adopt genetically modified insect-resistant maize in Southwest China.","authors":"Yanfang Huang,Minglong Zhang,Xiangping Jia,Fengying Nie,Xianming Yang,Kongming Wu","doi":"10.1080/21645698.2025.2497909","DOIUrl":"https://doi.org/10.1080/21645698.2025.2497909","url":null,"abstract":"The invasion of fall armyworm poses a serious threat to global food security, necessitating the urgent promotion of environmentally friendly pest control technologies. As a key strategy in green pest control, the effectiveness of genetically modified (GM) insect-resistant crops largely depends on the extent of farmer acceptance. Based on survey data from 426 GM maize pilot farmers in Yunnan Province, this study employs an ordered Logit model to analyze the key factors influencing farmers' adoption intentions from a behavioral decision-making perspective. The results indicate that (1) cognition, value orientation, and social trust are the primary drivers of farmers' willingness to adopt GM maize; (2) the impact of risk perception is context-dependent, exerting a significant positive moderating effect on planting decisions among farmers with higher levels of social trust; (3) planting experience reinforces the effects of cognition and social trust and amplifies the role of value orientation. This study provides policy-relevant insights for the industrial promotion of GM crops and the green control of fall armyworms, with important implications for safeguarding national food security.","PeriodicalId":501763,"journal":{"name":"GM Crops & Food","volume":"33 1","pages":"381-397"},"PeriodicalIF":0.0,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144146194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
GM Crops & FoodPub Date : 2025-05-04DOI: 10.1080/21645698.2025.2499998
Wanzhen Xu,Arshad Ali
{"title":"The moderating role of environment-related technologies in the link between transportation infrastructure, agricultural, and environmental contamination in highly congested countries of the world.","authors":"Wanzhen Xu,Arshad Ali","doi":"10.1080/21645698.2025.2499998","DOIUrl":"https://doi.org/10.1080/21645698.2025.2499998","url":null,"abstract":"Densely populated countries have greater demand for agricultural output, greater vehicle usage, and busier traffic, so these countries devote more agricultural land to agricultural production and invest more in transportation infrastructure, resulting in higher environmental emissions. Most studies in literature assume that environment-related technologies directly diminish environmental contamination, while this study suggests that environment-related technologies indirectly mitigate environmental hazards through interactions with agricultural land use, agricultural value addition, and transportation infrastructure. Thus, this study determines to disclose the influence of agricultural land use, agricultural value added, and transportation infrastructure investment on ecological damage in countries with the highest population density during the period 1990-2022; and the sole moderating effect of environment-related technologies in the association of the proposed regressors with CO2 emissions. Results illustrate that agricultural land use and investment in transportation infrastructure contribute significantly to CO2 emissions, but only in the long term. However, agriculture value addition strongly promotes environmental contamination in both the short and long term. Furthermore, in the long run, the reversed U-designed EKC premise holds only for the top five most populous countries. Environmental contamination can be mitigated through environmentally relevant technologies when interacting with agriculture land use, value addition of agricultural and investment in transportation infrastructure. Finally, (>-1.115) is the estimated threshold level of environment-related technologies, at which agricultural land use, agricultural value added, and transportation infrastructure can significantly improve environmental quality. Policymakers in densely populated countries should prioritize the adoption of environmentally friendly technologies in agriculture and transportation to achieve environmental sustainability.","PeriodicalId":501763,"journal":{"name":"GM Crops & Food","volume":"47 1","pages":"340-359"},"PeriodicalIF":0.0,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143915238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
GM Crops & FoodPub Date : 2025-05-02DOI: 10.1080/21645698.2025.2499997
Rosalyn B Angeles-Shim,Georgina V Vergara
{"title":"Is the Philippines ready for the large-scale cultivation of Bt cotton?","authors":"Rosalyn B Angeles-Shim,Georgina V Vergara","doi":"10.1080/21645698.2025.2499997","DOIUrl":"https://doi.org/10.1080/21645698.2025.2499997","url":null,"abstract":"The Philippine Bureau of Plant Industry released on August 24, 2023, a biosafety permit approving the commercial propagation of Bt cotton in the country. Bt cotton is genetically engineered to have built-in resistance to bollworm, the most destructive pest of the cotton plant. With the issuance of the permit and after more than two decades of economic slump, the Philippines is now gearing towards a renaissance of the cotton industry. But is the Philippines ready to implement the agri-technological innovation that is the Bt cotton? This paper provides insights into the promise of the proposed large-scale adoption of Bt cotton, as well as a commentary on the potential challenges that can impede the long-awaited revival of the cotton industry in this island country. Both challenges and potential solutions are presented in the context of institutional, financial and technical capacity of the Philippines to support the wide scale Bt cotton initiative.","PeriodicalId":501763,"journal":{"name":"GM Crops & Food","volume":"43 1","pages":"360-365"},"PeriodicalIF":0.0,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143903095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
GM Crops & FoodPub Date : 2025-01-17DOI: 10.1080/21645698.2024.2448869
Chunlai Wang,Yangyang Zhou,Yimeng Wang,Peng Jiao,Siyan Liu,Shuyang Guan,Yiyong Ma
{"title":"CRISPR-Cas9-mediated editing of ZmPL1 gene improves tolerance to drought stress in maize.","authors":"Chunlai Wang,Yangyang Zhou,Yimeng Wang,Peng Jiao,Siyan Liu,Shuyang Guan,Yiyong Ma","doi":"10.1080/21645698.2024.2448869","DOIUrl":"https://doi.org/10.1080/21645698.2024.2448869","url":null,"abstract":"Maize (Zea mays L.) is a widely grown food crop around the world. Drought stress seriously affects the growth and development process of plants and causes serious damage to maize yield. In the early stage, our research group conducted transcriptome sequencing analysis on the drought-resistant maize inbred line H8186 and screened out a gene with significantly down-regulated expression, Phylloplanin-like (ZmPL1). The ZmPL1 gee expression pattern was analyzed under various abiotic stresses, and the results showed that this gene was greatly affected by drought stress. Subcellular localization analysis showed that the protein was localized on the cell membrane. In order to verify the role of ZmPL1 in drought stress, we overexpressed ZmPL1 in yeast and found that the expression of ZmPL1 could significantly increase the drought sensitivity of yeast. Next, ZmPL1 transgenic plants were obtained by infecting maize callus using Agrobacterium-mediated method. Under drought stress, compared with overexpression lines, gene-edited lines had higher germination rate and seedling survival rate, lower accumulation of MDA, relative conductivity and ROS, higher antioxidant enzyme activity, and the expression levels of stress-related genes and ROS scavenging-related genes were significantly increased. Exogenous application of ABA to each lines under drought stress attenuated the damage caused by drought stress on ZmPL overexpressing plants. In summary, ZmPL1 negatively regulates drought tolerance in maize.","PeriodicalId":501763,"journal":{"name":"GM Crops & Food","volume":"20 1","pages":"1-16"},"PeriodicalIF":0.0,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142988498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
GM Crops & FoodPub Date : 2025-01-15DOI: 10.1080/21645698.2025.2450852
Hussein F Hassan,Hasan Yassine,Ahlam Chaaban,Ahmad Chehaitly,Zeinab Skaineh,Nagham Cherri,Sahar Moussawi,Nour Baytamouni,Philippe Hussein Kobeissy,Hani Dimassi,José-Noel Ibrahim
{"title":"Public knowledge, attitudes, and perceptions toward genetically modified foods in Lebanon.","authors":"Hussein F Hassan,Hasan Yassine,Ahlam Chaaban,Ahmad Chehaitly,Zeinab Skaineh,Nagham Cherri,Sahar Moussawi,Nour Baytamouni,Philippe Hussein Kobeissy,Hani Dimassi,José-Noel Ibrahim","doi":"10.1080/21645698.2025.2450852","DOIUrl":"https://doi.org/10.1080/21645698.2025.2450852","url":null,"abstract":"Genetically modified foods (GMFs) have garnered significant attention due to their implications for health, agriculture, and food security. Understanding knowledge and perceptions of GMFs is essential, as these factors influence acceptance and attitudes. This study, the first of its kind in Lebanon, aimed to assess the knowledge, attitudes, and perceptions regarding GMFs and to explore their association with socio-demographic characteristics. A cross-sectional study was conducted with 1,001 participants who completed a 50-item questionnaire, followed by a 15-minute educational session on GMFs. Statistical analysis was performed using SPSS. Prior to the educational session, participants had an average knowledge score of 60.3 ± 17.4%, which significantly increased to 83.0 ± 15.8% afterward. Attitude and perception scores improved from an average of 30.3 ± 25.1% pre-intervention to 38.9 ± 12.4% post-intervention. Females demonstrated significantly higher knowledge scores, and educational attainment was positively correlated with knowledge levels both pre- and post-intervention. Younger participants and those in health-related fields scored higher before the intervention, while unemployed individuals scored lower. Additionally, higher educational levels and health-related educational backgrounds were linked to better attitudes and perceptions pre-intervention, while unemployment correlated with lower scores. Interestingly, multivariate regression analysis indicated that being under 35 years of age, having a health-related educational background, and holding a university degree were predictors of higher GMF knowledge. Consequently, individuals with initially lower knowledge level benefited the most from the educational intervention, exhibiting the greatest knowledge increases post-education. Our findings underscore the importance of targeted educational initiatives to bridge knowledge gaps and address misconceptions regarding GMFs.","PeriodicalId":501763,"journal":{"name":"GM Crops & Food","volume":"24 1","pages":"144-155"},"PeriodicalIF":0.0,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142988497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Distribution status of genetically modified soybeans from the United States and Canada to Japan in 2021 and 2022.","authors":"Keisuke Soga,Yu Hashimoto,Tomohiro Egi,Chie Taguchi,Satoko Yoshiba,Norihito Shibata,Kazunari Kondo,Reona Takabatake","doi":"10.1080/21645698.2024.2444048","DOIUrl":"https://doi.org/10.1080/21645698.2024.2444048","url":null,"abstract":"The number of authorized genetically modified (GM) soybeans has increased worldwide. In Japan, 34 GM soybeans containing single events and their stacked varieties have been approved as food. However, not all approved GM events are commercially cultivated or distributed. In this study, we evaluated domestically distributed samples from the United States (US) and Canada using 17 event-specific detection methods for GM soybeans. Identity-preserved (IP) soybean samples imported from the US and Canada, and non-IP samples from the US in 2021 and 2022 were analyzed. Four GM soybean events consisting of MON89788, A5547-127, MON87708, and DAS-44406 were detected in all lots in the non-IP samples. Furthermore, a single-kernel-based analysis was conducted to determine whether the detected GM soybean events are stacked. The results suggest that DAS-44406 is rapidly increasing, particularly as a single event among GM soybeans.","PeriodicalId":501763,"journal":{"name":"GM Crops & Food","volume":"14 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142887913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
GM Crops & FoodPub Date : 2024-04-23DOI: 10.1080/21645698.2024.2345401
Gideon Sadikiel Mmbando
{"title":"The Adoption of Genetically Modified Crops in Africa: the Public’s Current Perception, the Regulatory Obstacles, and Ethical Challenges","authors":"Gideon Sadikiel Mmbando","doi":"10.1080/21645698.2024.2345401","DOIUrl":"https://doi.org/10.1080/21645698.2024.2345401","url":null,"abstract":"ABSTRACT Genetically modified (GM) crops are the most important agricultural commodities that can improve the yield of African smallholder farmers. The intricate circumstances surrounding the introduction of GM agriculture in Africa, however, underscore the importance of comprehending the moral conundrums, regulatory environments, and public sentiment that exist today. This review examines the current situation surrounding the use of GM crops in Africa, focusing on moral conundrums, regulatory frameworks, and public opinion. Only eleven of the fifty-four African countries currently cultivate GM crops due to the wide range of opinions resulting from the disparities in cultural, socioeconomic, and environmental factors. This review proposed that addressing public concerns, harmonizing regulations, and upholding ethical standards will improve the adoption of GM crops in Africa. This study offers ways to enhance the acceptability of GM crops for boosting nutrition and food security globally.","PeriodicalId":501763,"journal":{"name":"GM Crops & Food","volume":"60 6","pages":"1 - 15"},"PeriodicalIF":0.0,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140668055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}