GM Crops & Food最新文献_第2页

Transgenic barley over-expressing Aspergillus niger phytase phyA in field trials. 转基因大麦过表达黑曲霉植酸酶phyA的田间试验。

GM Crops & Food Pub Date : 2025-09-15 DOI: 10.1080/21645698.2025.2559488

T Vlčko,V Psota,R Koprna,W Harwood,L Ohnoutková

{"title":"Transgenic barley over-expressing Aspergillus niger phytase phyA in field trials.","authors":"T Vlčko,V Psota,R Koprna,W Harwood,L Ohnoutková","doi":"10.1080/21645698.2025.2559488","DOIUrl":"https://doi.org/10.1080/21645698.2025.2559488","url":null,"abstract":"Phytic acid is the main storage of phosphate in grains of staple crops. As phytic acid is hardly digestible for non-ruminants microbial phytases are used to supplement animal feed to enhance phosphate digestibility. A fungal phytase gene was introduced into barley with the aim of enhancing phosphate digestibility. Transgenic homozygous barley over-expressing fungal phytase phyA showed a 3.3fold increase in mature grain phytase activity. Field trials at two locations in the Czech Republic were conducted in a five-year experiment to test transgene stability and activity under field conditions. Increased phytase activity gradually decreased over the generations showing the most significant drop in the initial years of field trials. Molecular analysis revealed methylation in the coding sequence of the phyA transgene, suggesting transcription gene silencing. On the other hand, herbicide resistance used for selection of transgenic plants was functional over all generations. The feasibility of crossing the transgene into the feeding cultivar Azit was demonstrated with subsequent stabilization of hybrid progeny through androgenesis. Our results indicate that the Azit genetic background tended to reduce phytase activity in mature grains of hybrids. Grain-specific over-expression of fungal phytase driven by an amylase promoter improved phosphate levels during germination. Unfortunately, a malting experiment revealed that phytase over-expression did not significantly improve malting parameters. In fact, the higher nitrogen content in unmalted grain negatively affected the quality of the malt produced from them.","PeriodicalId":501763,"journal":{"name":"GM Crops & Food","volume":"3 1","pages":"607-625"},"PeriodicalIF":0.0,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145059049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifaceted roles and regulatory mechanisms of MYB transcription factors in plant development, secondary metabolism, and stress adaptation: current insights and future prospects. MYB转录因子在植物发育、次生代谢和逆境适应中的多重作用和调控机制：目前的认识和未来展望

GM Crops & Food Pub Date : 2025-09-15 DOI: 10.1080/21645698.2025.2559489

Muhammad Imran,Qiufei Wu,Chen Guanming,Lixia Zhou

{"title":"Multifaceted roles and regulatory mechanisms of MYB transcription factors in plant development, secondary metabolism, and stress adaptation: current insights and future prospects.","authors":"Muhammad Imran,Qiufei Wu,Chen Guanming,Lixia Zhou","doi":"10.1080/21645698.2025.2559489","DOIUrl":"https://doi.org/10.1080/21645698.2025.2559489","url":null,"abstract":"MYB transcription factor family represents one of the largest and most functionally diverse groups of regulatory proteins in plants, playing a crucial role in controlling genes involved in growth, development, and stress responses. MYB proteins are characterized by a conserved N-terminal DNA-binding domain. They are classified based on the number of R repeats, and possess a variable C-terminal region that determines their specific functions. In response to environmental signals, MYB proteins bind to specific DNA elements in target promoters, acting alone or with other regulators to modulate stress-responsive pathways. These factors integrate signaling cascades involving abscisic acid (ABA), jasmonic acid (JA), brassinosteroids (BR), and reactive oxygen species (ROS), aiding plant adaptation to adverse conditions. This review explores structural features, classification, and regulatory mechanisms, focusing on their roles in salinity, drought, extreme temperatures, nutrient deficiencies, heavy metal toxicity, and pathogen defense. Additionally, we highlight the advances and potential of MYB genes as targets for engineering stress-resilient crops through breeding and genetic modification.","PeriodicalId":501763,"journal":{"name":"GM Crops & Food","volume":"65 1","pages":"626-655"},"PeriodicalIF":0.0,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145068368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relative safety of glyphosate-resistant maize (CC-2) in rats. 抗草甘膦玉米（CC-2）在大鼠体内的相对安全性

GM Crops & Food Pub Date : 2025-09-12 DOI: 10.1080/21645698.2025.2550820

Anyi Liu,Xueqian Yin,Jing Wen,Chao Hou,Ruoyu Zhou,Xinran Liu,Ning Yin,Yuanzhi Jian,Shan Liu,Xiaoxuan Zhang,Junbo Wang

引用次数: 0

Overexpression of Leptochloa fusca H + -pyrophosphatase ( LfVP1 ) gene improves salinity and drought tolerance in tobacco 过表达褐藻H + -焦磷酸酶（LfVP1）基因提高烟草耐盐性和耐旱性

GM Crops & Food Pub Date : 2025-09-02 DOI: 10.1080/21645698.2025.2555037

Muhammad Rauf, Khurram Shahzad, Imran Habib, Badr Alharthi, Sajid Fiaz, Mehwish Kanwal, Moddassir Ahmed, Rashid Ali, Gerald A Berkowitz, Asjad Ali, Seung Hwan Yang, Nasir A. Saeed

引用次数: 0

To patent or not to patent: challenges and solutions to the patent ban on new genomic techniques plant in EU 申请或不申请专利：欧盟新基因组技术植物专利禁令的挑战与解决方案

GM Crops & Food Pub Date : 2025-08-31 DOI: 10.1080/21645698.2025.2548638

Li Jiang, Mike Adcock, Hong Yang

引用次数: 0

Genome-wide profiling of GRAS genes in flax ( Linum usitatissimum L.) reveals LuGRAS30 as a key regulator of drought stress resistance 对亚麻（Linum usitatissimum L.） GRAS基因的全基因组分析表明，LuGRAS30是亚麻抗旱性的关键调控因子

GM Crops & Food Pub Date : 2025-08-25 DOI: 10.1080/21645698.2025.2548639

Yihang Bao, Chulin Pan

引用次数: 0

Development of a duplex chamber digital PCR to quantify twelve genetically modified maize events 建立双室数字PCR定量12个转基因玉米事件

GM Crops & Food Pub Date : 2025-08-22 DOI: 10.1080/21645698.2025.2548053

Eun-Ha Kim, Youn-Sung Cho, Byeori Kim, Yelinn Yoo, Jae-In Lee, Young Soon Kim, Tae-Sung Park

引用次数: 0

Effect of prolonged cooking on pro-vitamin A levels of biofortified East African highland bananas 长时间烹调对生物强化东非高原香蕉维生素A原含量的影响

GM Crops & Food Pub Date : 2025-07-09 DOI: 10.1080/21645698.2025.2529637

Stephen Buah, Janefer Kiwummulo, Jean-Yves Paul, Joel Walugembe, Jackline Wegesa, Robooni Tumuhimbise, Wilberforce Tushemereirwe

引用次数: 0

CRISPR/Cas9-mediated editing of barley lipoxygenase genes promotes grain fatty acid accumulation and storability. CRISPR/ cas9介导的大麦脂氧合酶基因编辑促进了谷物脂肪酸的积累和储存能力。

GM Crops & Food Pub Date : 2025-06-26 DOI: 10.1080/21645698.2025.2523069

Zhanghui Zeng,Huiling Wang,Yingjie Luo,Wenjun Chen,Mingrui Xu,Haonan Wei,Zhehao Chen,Taihe Xiang,Lilin Wang,Ning Han,Xiaoping Huang,Hongwu Bian

{"title":"CRISPR/Cas9-mediated editing of barley lipoxygenase genes promotes grain fatty acid accumulation and storability.","authors":"Zhanghui Zeng,Huiling Wang,Yingjie Luo,Wenjun Chen,Mingrui Xu,Haonan Wei,Zhehao Chen,Taihe Xiang,Lilin Wang,Ning Han,Xiaoping Huang,Hongwu Bian","doi":"10.1080/21645698.2025.2523069","DOIUrl":"https://doi.org/10.1080/21645698.2025.2523069","url":null,"abstract":"Plant lipoxygenases (LOXs) catalyze the oxidation of polyunsaturated fatty acids, which can adversely affect grain storability. Although the genetic engineering of LOXs holds great potential for improving grain storage quality, this approach remains largely unexplored in barley. In this study, we identified five LOX genes in the barley genome: HvLOXA, HvLOXB, and HvLOXC1-3. HvLOXC1 exhibited the highest expression in early developing grains, roots, and shoots; HvLOXA was predominantly expressed in embryos, whereas HvLOXB and HvLOXC3 were weakly expressed across tissues. Transgene-free homozygous barley mutants of loxB, loxC1, and loxAloxC1 were generated using CRISPR/Cas9-mediated genome editing. Compared to the wild-type, all mutants displayed normal plant height, tiller number, and grain size, although the loxC1 and loxAloxC1 mutants exhibited significantly lower thousand grain weights. Notably, the total LOX activity in mature grains decreased by 36-42% in loxC1 mutants and by 94% in loxAloxC1 mutants, with no significant change observed in loxB mutants. Additionally, the loxAloxC1 double mutants had a significantly lower malondialdehyde content and accumulated 10-21% more fatty acids than the wild-type. Artificial aging treatment experiments revealed that loxAloxC1 mutants had enhanced grain storability, demonstrated by significantly higher germination rates, reduced lipid peroxidation, and improved seedling growth. Our findings highlight that the targeted knockout of LOX genes, particularly the double mutation of HvLOXA and HvLOXC1, represents a promising genetic strategy for improving grain storability and nutritional value in barley.","PeriodicalId":501763,"journal":{"name":"GM Crops & Food","volume":"43 1","pages":"482-497"},"PeriodicalIF":0.0,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144488182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Feasibility of a safe innovation framework for crop breeding. 作物育种安全创新框架的可行性。

GM Crops & Food Pub Date : 2025-06-26 DOI: 10.1080/21645698.2025.2524236

Marc Groenen,Gijs W Spaans,Lianne M S Bouwman,Gijs A Kleter,Jan Pieter van der Berg

引用次数: 0