GM Crops & Food最新文献

The NtBAG5-CaM complex integrates Ca2+ signals to regulate leaf senescence via the antioxidant system in tobacco. NtBAG5-CaM复合物通过烟草抗氧化系统整合Ca2+信号调控叶片衰老。

GM Crops & Food Pub Date : 2025-10-24 DOI: 10.1080/21645698.2025.2578048

Langlang Zhang,Bing Hou,Xiao Chen,Xinghui Liu,Wenxin Xie,Xinxiang Chen,Mingli Chen,Minmin Xie,Jili Zhang,Daping Gong,Quan Sun

{"title":"The NtBAG5-CaM complex integrates Ca2+ signals to regulate leaf senescence via the antioxidant system in tobacco.","authors":"Langlang Zhang,Bing Hou,Xiao Chen,Xinghui Liu,Wenxin Xie,Xinxiang Chen,Mingli Chen,Minmin Xie,Jili Zhang,Daping Gong,Quan Sun","doi":"10.1080/21645698.2025.2578048","DOIUrl":"https://doi.org/10.1080/21645698.2025.2578048","url":null,"abstract":"Leaf yellowing critically impacts tobacco quality and economic value. The Bcl-2-associated athanogene (BAG) gene family regulates plant development and senescence, yet the role of NtBAG5 in tobacco remains poorly understood. Here, we demonstrate that NtBAG5 acts as a key promoter of leaf senescence. CRISPR/Cas9-generated NtBAG5 mutants exhibited delayed senescence, enhanced activities of antioxidant enzymes, and reduced malondialdehyde (MDA) content, whereas NtBAG5-overexpressing plants showed the opposite effects. Promoter-GUS analysis revealed high NtBAG5 expression in roots and stems. Hormone treatments indicated that NtBAG5 expression is upregulated by ABA, ETH, IAA, and GA (at late stage) but downregulated by MeJA. Mechanistically, NtBAG5 interacts with calmodulin (CaM) via its BAG domain and IQ motif, as confirmed by yeast two-hybrid and BiFC assays. Ca2 + was found to modulate CaM conformation and strengthen the NtBAG5-CaM interaction. Silencing NtCaM via VIGS induced severe leaf yellowing and growth defects. Our results reveal that the NtBAG5-CaM complex, regulated by Ca2 + and hormones, modulates leaf senescence through the antioxidant system, providing new insights for improving tobacco quality.","PeriodicalId":501763,"journal":{"name":"GM Crops & Food","volume":"39 1","pages":"837-851"},"PeriodicalIF":0.0,"publicationDate":"2025-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145351790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An environmental risk assessment of maize containing event, DP-Ø51291-2, with activity against corn rootworms (Diabrotica spp.) via expression of the protein, IPD072Aa. 通过表达IPD072Aa蛋白对玉米根虫（Diabrotica spp.）具有抗虫活性的事件蛋白DP-Ø51291-2进行环境风险评估。

GM Crops & Food Pub Date : 2025-10-22 DOI: 10.1080/21645698.2025.2572192

Kursten A Spegar,Bridget F O'Neill,Veríssimo Sá,Kristine LeRoy,Emily Moellring,Sarah Brustkern,Reba Bruyere,Taylor Olson,Tim Gunderson,Philip Utley,Anne B Carlson,Rachel Woods,Brian Stolte,Chris Linderblood,Nick Schmidt

{"title":"An environmental risk assessment of maize containing event, DP-Ø51291-2, with activity against corn rootworms (Diabrotica spp.) via expression of the protein, IPD072Aa.","authors":"Kursten A Spegar,Bridget F O'Neill,Veríssimo Sá,Kristine LeRoy,Emily Moellring,Sarah Brustkern,Reba Bruyere,Taylor Olson,Tim Gunderson,Philip Utley,Anne B Carlson,Rachel Woods,Brian Stolte,Chris Linderblood,Nick Schmidt","doi":"10.1080/21645698.2025.2572192","DOIUrl":"https://doi.org/10.1080/21645698.2025.2572192","url":null,"abstract":"Maize event DP-Ø51291-2 expresses the protein, IPD072Aa, which is derived from Pseudomonas chlororaphis, encoded by the ipd072Aa gene, to provide corn rootworm control. An environmental risk assessment was conducted for DP-Ø51291-2 maize which characterized potential exposure and hazard of the IPD072Aa protein to non-target organisms (NTOs). To estimate potential exposure to the IPD072Aa protein, worst-case estimated environmental concentrations (EECs) and refined EECs, where applicable, were calculated. To characterize potential hazard from the IPD072Aa protein, laboratory dietary toxicity studies were conducted with surrogate NTOs representing functional groups selected via problem formulation. Margins of exposure for each surrogate species were determined by comparing hazard and exposure values which indicated negligible potential risk to NTO populations. To add an additional line of evidence, a field assessment was conducted for DP-Ø51291-2 maize as compared to a near isoline control. Overall, no unreasonable adverse effects to NTO populations are anticipated from DP-Ø51291-2 maize cultivation.","PeriodicalId":501763,"journal":{"name":"GM Crops & Food","volume":"47 1","pages":"811-836"},"PeriodicalIF":0.0,"publicationDate":"2025-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145339087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environmental risk assessment of the Cry1B.34.1, Cry1B.61.1, and IPD083Cb proteins to non-target organisms. Cry1B.34.1、Cry1B.61.1和IPD083Cb蛋白对非靶生物的环境风险评价

GM Crops & Food Pub Date : 2025-10-17 DOI: 10.1080/21645698.2025.2572186

Joseph E Iboyi,Chad J Boeckman,Kristine LeRoy,Stephen L Ballou,Reba J Bruyere,Verissimo G de Sá,Timothy Gunderson,Emily Huang,Christopher Linderblood,Taylor Olson,Bridget F O'Neill,Josh J Parsons,Mathew Sandmann,Brian Stolte,Yiwei Wang

{"title":"Environmental risk assessment of the Cry1B.34.1, Cry1B.61.1, and IPD083Cb proteins to non-target organisms.","authors":"Joseph E Iboyi,Chad J Boeckman,Kristine LeRoy,Stephen L Ballou,Reba J Bruyere,Verissimo G de Sá,Timothy Gunderson,Emily Huang,Christopher Linderblood,Taylor Olson,Bridget F O'Neill,Josh J Parsons,Mathew Sandmann,Brian Stolte,Yiwei Wang","doi":"10.1080/21645698.2025.2572186","DOIUrl":"https://doi.org/10.1080/21645698.2025.2572186","url":null,"abstract":"Lepidopteran pests are a serious threat to soybean production worldwide and have developed resistance to several pest management options, including the use of transgenic soybean expressing crystalline (Cry) proteins derived from the soil bacterium Bacillus thuringiensis Berliner (Bt). For this reason, there is great interest in discovering insecticidal proteins that function via new modes and/or sites of action against lepidopteran pests to support a sustainable and durable management plan. Event COR-23134-4 (hereafter referred to as COR23134 soybean), which expresses Bt-derived insecticidal proteins Cry1B.34.1 and Cry1B.61.1 and a novel plant-derived IPD083Cb insecticidal protein, was developed to provide additional sites of action to confer protection against certain susceptible lepidopteran pests. As part of the environmental safety assessment, the potential risks (exposure and hazard) posed by the cultivation of COR23134 soybean to non-target organisms (NTOs) were assessed. The environmental risk was characterized by comparing the Tier I laboratory hazard study results to worst-case or refined estimated environmental concentrations (EECs) to establish the margin of exposure (MOE) for the Cry1B.34.1, Cry1B.61.1, and IPD083Cb proteins in COR23134 soybean. Overall, results from the exposure and hazard assessments and the MOE values show that the Cry1B.34.1, Cry1B.61.1, and IPD083Cb insecticidal proteins expressed in COR23134 soybean are not expected to result in unreasonable adverse effects on NTO populations at environmentally realistic concentrations; hence, the risk to NTOs from the cultivation of COR23134 soybean is considered negligible.","PeriodicalId":501763,"journal":{"name":"GM Crops & Food","volume":"3 1","pages":"733-759"},"PeriodicalIF":0.0,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145305654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Safety assessment of membrane-associated protoporphyrinogen IX oxidase from Enterobacter cloacae expressed in genetically modified crops. 阴沟肠杆菌膜相关原卟啉原IX氧化酶在转基因作物中表达的安全性评价。

GM Crops & Food Pub Date : 2025-10-16 DOI: 10.1080/21645698.2025.2572188

Cunxi Wang,Wenze Li,Matias S Attene-Ramos,Colton Kessenich,Jennifer Calcaterra,Rong Wang,Bosong Xiang,Sergio Couoh-Cardel,Kimberly Hodge-Bell,Scott A Saracco

{"title":"Safety assessment of membrane-associated protoporphyrinogen IX oxidase from Enterobacter cloacae expressed in genetically modified crops.","authors":"Cunxi Wang,Wenze Li,Matias S Attene-Ramos,Colton Kessenich,Jennifer Calcaterra,Rong Wang,Bosong Xiang,Sergio Couoh-Cardel,Kimberly Hodge-Bell,Scott A Saracco","doi":"10.1080/21645698.2025.2572188","DOIUrl":"https://doi.org/10.1080/21645698.2025.2572188","url":null,"abstract":"Crops tolerant to protoporphyrinogen IX oxidase (PPO)-inhibiting herbicides were developed by expressing an herbicide insensitive PPO, a membrane-associated protein from Enterobacter cloacae. E. cloacae is commonly found in the environment, and PPO is present in thousands of species, including probiotic organisms with a history of safe use. Comparisons with current allergen and protein toxin databases revealed no sequence similarities between PPO and known allergens or toxins. To validate the use of Escherichia coli-produced PPO in safety studies, physicochemical and functional characterization demonstrated that the PPO produced by genetically modified (GM) soybean has comparable immunoreactivity and functional activity to that produced from E. coli, with neither being glycosylated. PPO was fully digested after exposure to pepsin and pancreatin for 2 and 5 minutes, respectively, and its activity is completely lost at temperatures of 55°C or higher. Mice dosed orally with PPO at a level of 5000 mg protein per kg body weight showed no adverse effects, as indicated in body weight gains, food consumption, and clinical observations. This comprehensive safety assessment indicates that PPO protein from GM crops is safe for food and feed consumption. Additionally, we present methods to demonstrate the functional equivalence of a membrane-associated protein from E. coli and plant, along with a novel process for formulating PPO in gram quantities at 76.3 mg/ml.","PeriodicalId":501763,"journal":{"name":"GM Crops & Food","volume":"40 1","pages":"760-778"},"PeriodicalIF":0.0,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145305655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of the public perception and acceptance of gene-editing technology and gene-edited agricultural products in South Korea. 韩国公众对基因编辑技术和基因编辑农产品的认知和接受度分析。

GM Crops & Food Pub Date : 2025-10-16 DOI: 10.1080/21645698.2025.2576272

Sung-Dug Oh,Bumkyu Lee

{"title":"Analysis of the public perception and acceptance of gene-editing technology and gene-edited agricultural products in South Korea.","authors":"Sung-Dug Oh,Bumkyu Lee","doi":"10.1080/21645698.2025.2576272","DOIUrl":"https://doi.org/10.1080/21645698.2025.2576272","url":null,"abstract":"Genome editing (GE) is a promising agricultural technology; however, its effective adoption relies on safety assurance and public trust. To investigate Korean perceptions, a 2national survey (n = 1,055) was conducted in 2024 on awareness, attitudes, acceptance, and information behavior. Awareness was high for familiar terms such as \"gene scissors,\" but low for scientific terms such as CRISPR. Willingness to purchase GE products was 70%, exceeding that for GMO reported previously, although respondents favored conditional adoption (research, imports) over domestic cultivation. Safety was the most decisive factor, not only in the form of scientific verification but also in transparency and institutional safeguards. Expert organizations were trusted, yet mass media remained the preferred information channels, revealing a credibility - accessibility gap. Respondents also emphasized expert and government leadership in policymaking. Overall, Korean perceptions align with global patterns but show stronger emphasis on policy trust and media reliance, underscoring the need for transparent safety verification, expert-led yet mass-mediated communication, and tailored strategies.","PeriodicalId":501763,"journal":{"name":"GM Crops & Food","volume":"46 1","pages":"795-810"},"PeriodicalIF":0.0,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145305656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolite profiling to evaluate metabolic changes in drought-tolerant transformant (Agb0103) under salt stress 盐胁迫下耐旱转化体Agb0103代谢变化的代谢物分析

GM Crops & Food Pub Date : 2025-10-14 DOI: 10.1080/21645698.2025.2569162

Gyeong-Min Lee, Sung-Dug Oh, Ye-Jin Jang, Jong-Chan Park, Hyun-Min Park, Seon-Woo Oh, Sang-Gu Lee, Nam-Jin Chung, Soo-Yun Park, An-Cheol Chang

引用次数: 0

Advancing nutritional quality in oilseed crops through genome editing: a comprehensive review. 通过基因组编辑提高油籽作物营养品质：综述

GM Crops & Food Pub Date : 2025-10-13 DOI: 10.1080/21645698.2025.2572864

Asmamaw Menelih,Abayeneh Girma,Aleka Aemiro

引用次数: 0

How labeling of genetically modified foods affects consumers' purchase intentions: a multi-contextual analysis. 转基因食品的标签如何影响消费者的购买意愿：一个多背景分析。

GM Crops & Food Pub Date : 2025-10-08 DOI: 10.1080/21645698.2025.2572191

Zheng Yang,Yingdi Jiang,Yun Feng,Guoyan Wang

{"title":"How labeling of genetically modified foods affects consumers' purchase intentions: a multi-contextual analysis.","authors":"Zheng Yang,Yingdi Jiang,Yun Feng,Guoyan Wang","doi":"10.1080/21645698.2025.2572191","DOIUrl":"https://doi.org/10.1080/21645698.2025.2572191","url":null,"abstract":"As a critical carrier for ensuring consumer right-to-know and facilitating risk communication, the effectiveness of genetically modified (GM) labeling is influenced by cognitive biases, yet its behavioral impact remains underexplored, particularly in non-Western contexts. Through a dual-context online experiment (edible soybean oil vs. non-edible cotton, n = 800) conducted in China, this study examines how GM labeling affects purchase intentions, incorporating the roles of risk perception and moderating effect of metacognitive bias. The results reveal that risk perception mediates this relationship, while metacognitive ability significantly moderates it. More importantly, the results indicate that the audiences with different cognitive characteristics also have diverse cognitive effects and psychological pathways toward similar GM labels. These findings surpass traditional \"knowledge-attitude\" linear paradigms and further offer practical insights for policymakers: tailored GM labeling regulations and segmented communication strategies should be developed based on cognitive typologies to improve consumer understanding and decision-making.","PeriodicalId":501763,"journal":{"name":"GM Crops & Food","volume":"15 1","pages":"688-708"},"PeriodicalIF":0.0,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145246604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Promoting biotechnology applications in China through effective biosafety education and communication 通过有效的生物安全教育和宣传，促进生物技术在中国的应用

GM Crops & Food Pub Date : 2025-10-08 DOI: 10.1080/21645698.2025.2569930

Ying Wang, Bao-Rong Lu

引用次数: 0

Impact of genetically modified Brinjal (Bt brinjal) on farmers' income and production in Pabna District, Bangladesh. 转基因茄子（Bt茄子）对孟加拉国Pabna地区农民收入和生产的影响。

GM Crops & Food Pub Date : 2025-09-22 DOI: 10.1080/21645698.2025.2560698

Md Amzad Hossain,Kazi Khalid Hossain,Niraj Prakash Joshi

{"title":"Impact of genetically modified Brinjal (Bt brinjal) on farmers' income and production in Pabna District, Bangladesh.","authors":"Md Amzad Hossain,Kazi Khalid Hossain,Niraj Prakash Joshi","doi":"10.1080/21645698.2025.2560698","DOIUrl":"https://doi.org/10.1080/21645698.2025.2560698","url":null,"abstract":"Approval of Bt brinjal cultivation represents a crucial step forward for Bangladesh in agricultural biotechnology. However, the scalability of Bt brinjal adoption faces barriers, mainly due to resistance from traditional farmers, the limited evidence suggesting its socioeconomic impacts and full realization of its benefits. This study evaluates the socio-economic impacts of Bt brinjal adoption in the Pabna District. The study analyzed the impacts based on data from 489 brinjal farmers, comprising 197 adopters of Bt brinjal and 292 non-adopters employing propensity score matching, a method that helps to reduce selection bias in observational studies. The findings reveal that Bt brinjal adoption increased brinjal yield by 5,845.33 kg per hectare and raised profits by 226,577.54 BD taka (equivalent to 1,884.95 USD) per hectare. Additionally, pesticide costs were reduced by 41,269.499 BD taka (equivalent to 343.38 USD) per hectare. The increased yield and income and reduced use of pesticides demonstrate the economic and environmental advantages of Bt brinjal adoption. To harness the full potential of Bt brinjal, policymakers could adopt strategies that enhance farmers' access to Bt brinjal technology and disseminate its positive socio-economic and environmental advantages through targeted educational programs. Such initiatives encourage widespread adoption and contribute to sustainable growth in the agricultural sector.","PeriodicalId":501763,"journal":{"name":"GM Crops & Food","volume":"98 4 1","pages":"656-673"},"PeriodicalIF":0.0,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145103540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0