npj Imaging最新文献_第4页

PET imaging of mycobacterial infection: transforming the pipeline for tuberculosis drug development. 分枝杆菌感染的PET显像：改变结核病药物开发的管道。

npj Imaging Pub Date : 2025-05-28 DOI: 10.1038/s44303-025-00082-2

Janke Kleynhans, Christiaan A Gouws, Thomas Ebenhan

引用次数: 0

3D imaging of the human temporal bone by X-ray phase-contrast tomography. 人类颞骨的x射线相衬断层扫描三维成像。

npj Imaging Pub Date : 2025-05-20 DOI: 10.1038/s44303-025-00086-y

Jannis J Schaeper, Paul Tafforeau, Christoph A Kampshoff, Carolina Thomas, Alexander Meyer, Christine Stadelmann, M Charles Liberman, Tobias Moser, Tim Salditt

引用次数: 0

Spillover can limit accurate signal quantification in MPI. 在MPI中，溢出效应会限制信号的精确量化。

npj Imaging Pub Date : 2025-05-06 DOI: 10.1038/s44303-025-00084-0

Ali Shakeri-Zadeh, Shreyas Kuddannaya, Adnan Bibic, Jeff W M Bulte

引用次数: 0

Temperature dependent chemical shifts of pyruvate and lactate enable in vivo hyperpolarized ¹³C MRSI thermometry. 丙酮酸盐和乳酸盐的温度依赖化学位移使体内超极化13C磁共振成像测温成为可能。

npj Imaging Pub Date : 2025-05-06 DOI: 10.1038/s44303-025-00081-3

Wolfgang Gottwald, Luca Nagel, Jason G Skinner, Martin Grashei, Sandra Sühnel, Nadine Setzer, Wolfgang Eisenreich, Mary A McLean, Ferdia A Gallagher, Jae Mo Park, Zumrud Ahmadova, Martin Gierse, Senay Karaali, Stephan Knecht, Ilai Schwartz, Irina Heid, Geoffrey J Topping, Frits H A van Heijster, Franz Schilling

{"title":"Temperature dependent chemical shifts of pyruvate and lactate enable in vivo hyperpolarized 13C MRSI thermometry.","authors":"Wolfgang Gottwald, Luca Nagel, Jason G Skinner, Martin Grashei, Sandra Sühnel, Nadine Setzer, Wolfgang Eisenreich, Mary A McLean, Ferdia A Gallagher, Jae Mo Park, Zumrud Ahmadova, Martin Gierse, Senay Karaali, Stephan Knecht, Ilai Schwartz, Irina Heid, Geoffrey J Topping, Frits H A van Heijster, Franz Schilling","doi":"10.1038/s44303-025-00081-3","DOIUrl":"10.1038/s44303-025-00081-3","url":null,"abstract":"The chemical shift of many molecules changes with temperature, which enables non-invasive magnetic resonance imaging (MRI) thermometry. Hyperpolarization methods increase the inherently low 13C MR signal. The commonly-used hyperpolarized probe [1-13C]pyruvate, and its metabolic product [1-13C]lactate, exhibit temperature and concentration dependent chemical shift changes that have not previously been reported. These effects were characterized at 7 T and 11.7 T in vitro and applied for in vivo thermometry both preclinically at 7 T and to human data at 3 T. Apparent temperature values from mouse abdomen and brain were similar to rectally measured temperature. Human brain and kidney apparent temperatures from 13C MRSI were lower than known physiological temperatures, suggesting that additional effects may currently limit the use of this method for determining absolute temperature in humans. The temperature dependent chemical shift changes also have implications for sequence design and for in vitro studies with hyperpolarized pyruvate.","PeriodicalId":501709,"journal":{"name":"npj Imaging","volume":"3 1","pages":"19"},"PeriodicalIF":0.0,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12118740/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144556395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Noninvasive assessment of liver inflammation in metabolic dysfunction associated steatohepatitis using MR cytometry. 利用磁共振细胞术无创评估代谢功能障碍相关脂肪性肝炎的肝脏炎症。

npj Imaging Pub Date : 2025-05-03 DOI: 10.1038/s44303-025-00080-4

Xiaoyu Jiang, Mary Kay Washington, Manhal J Izzy, Gracie Piantek, Ming Lu, Xinqiang Yan, John C Gore, Junzhong Xu

{"title":"Noninvasive assessment of liver inflammation in metabolic dysfunction associated steatohepatitis using MR cytometry.","authors":"Xiaoyu Jiang, Mary Kay Washington, Manhal J Izzy, Gracie Piantek, Ming Lu, Xinqiang Yan, John C Gore, Junzhong Xu","doi":"10.1038/s44303-025-00080-4","DOIUrl":"10.1038/s44303-025-00080-4","url":null,"abstract":"The current diagnostic gold standard for metabolic dysfunction-associated steatohepatitis (MASH) requires invasive biopsy to assess steatosis, inflammation, and ballooning. While MRI-based proton density fat fraction (PDFF) and MR elastography address steatosis and fibrosis, non-invasive methods for evaluating hepatic inflammation remain lacking. This study developed a diffusion MRI (dMRI)-based MR cytometry technique to map liver cellular properties, including MRI-derived cell size (excluding fat content) and cell density. Validation through histology-driven simulations and ex vivo MRI of fixed human liver specimens demonstrated that stromal regions exhibit smaller MRI-derived cell sizes and higher cell densities than both normal and fatty tissues. An in vivo feasibility study, conducted on healthy subjects (n = 5) and MASH patients (n = 5) using a clinical 3 T MRI system, further showcased the potential of MR cytometry to characterize pathological changes in liver microstructure.","PeriodicalId":501709,"journal":{"name":"npj Imaging","volume":"3 1","pages":"17"},"PeriodicalIF":0.0,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12118690/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144556391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Manganese-based type I collagen-targeting MRI probe for in vivo imaging of liver fibrosis. 基于锰的I型胶原靶向MRI探针用于肝纤维化的体内成像。

npj Imaging Pub Date : 2025-04-09 DOI: 10.1038/s44303-025-00075-1

Chunxiang Zhang, Hua Ma, Daniel DeRoche, Eric M Gale, Pamela Pantazopoulos, Nicholas J Rotile, Himashinie Diyabalanage, Valerie Humblet, Peter Caravan, Iris Y Zhou

{"title":"Manganese-based type I collagen-targeting MRI probe for in vivo imaging of liver fibrosis.","authors":"Chunxiang Zhang, Hua Ma, Daniel DeRoche, Eric M Gale, Pamela Pantazopoulos, Nicholas J Rotile, Himashinie Diyabalanage, Valerie Humblet, Peter Caravan, Iris Y Zhou","doi":"10.1038/s44303-025-00075-1","DOIUrl":"10.1038/s44303-025-00075-1","url":null,"abstract":"Liver fibrosis is a common pathway shared by all forms of progressive chronic liver disease. There is an unmet clinical need for noninvasive imaging tools to diagnose and stage fibrosis, which presently relies heavily on percutaneous liver biopsy. Here, we explored the feasibility of using a novel type I collagen-targeted manganese (Mn)-based MRI probe, Mn-CBP20, for liver fibrosis imaging. In vitro characterization of Mn-CBP20 demonstrated its high binding affinity for human collagen (Kd = 9.6 µM), high T1-relaxivity (48.9 mM-1 s-1 at 1.4 T and 27 °C), and kinetic inertness to Mn release under forcing conditions. We demonstrated MRI using Mn-CBP20 performs comparably to previously reported gadolinium-based type I collagen-targeted probe EP-3533 in a mouse model of carbon tetrachloride-induced liver fibrosis, and further demonstrate efficacy to detect fibrosis in a diet-induced mouse model of metabolically-associated steatohepatitis. Biodistribution studies using the Mn-CBP20 radiolabeled with the positron-emitting 52Mn isotope demonstrate efficient clearance of Mn-CBP20 primarily via renal excretion. Mn-CBP20 represents a promising candidate that merits further evaluation and development for molecular imaging of liver fibrosis.","PeriodicalId":501709,"journal":{"name":"npj Imaging","volume":"3 1","pages":"14"},"PeriodicalIF":0.0,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12118721/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144556389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fast and label-free 3D virtual H&E histology via active phase modulation-assisted dynamic full-field OCT. 通过有源相位调制辅助的动态全视野OCT，快速和无标签的3D虚拟H&E组织学。

npj Imaging Pub Date : 2025-04-08 DOI: 10.1038/s44303-025-00068-0

Zichen Yin, Bin He, Yuzhe Ying, Shuwei Zhang, Panqi Yang, Zhengyu Chen, Zhangwei Hu, Yejiong Shi, Ruizhi Xue, Chengming Wang, Shu Wang, Guihuai Wang, Ping Xue

{"title":"Fast and label-free 3D virtual H&E histology via active phase modulation-assisted dynamic full-field OCT.","authors":"Zichen Yin, Bin He, Yuzhe Ying, Shuwei Zhang, Panqi Yang, Zhengyu Chen, Zhangwei Hu, Yejiong Shi, Ruizhi Xue, Chengming Wang, Shu Wang, Guihuai Wang, Ping Xue","doi":"10.1038/s44303-025-00068-0","DOIUrl":"10.1038/s44303-025-00068-0","url":null,"abstract":"Pathological features are the gold standard for tumor diagnosis, guiding treatment and prognosis. However, the standard histopathological process is labor-intensive and time-consuming. Although frozen sections offer a quicker alternative, they often induce severe artifacts and suffer from lower accuracy. Dynamic full-field optical coherence tomography (D-FFOCT) is an innovative optical imaging technique that provides rapid histological information by utilizing subcellular dynamics as an intrinsic source of contrast. Despite its advantages, D-FFOCT images of adjacent tissues frequently exhibit abrupt shifts in hue and brightness, which is confusing for pathologists and diminishes its interpretability and reliability. Here, we present an active phase modulation-assisted D-FFOCT (APMD-FFOCT) to improve the imaging stability and achieve continuity and consistency in image stitching, which also enhances the image contrast of tissues with low metabolic dynamics. This enables us to further employ an unsupervised generative adversarial network to convert APMD-FFOCT images into virtual hematoxylin and eosin (H&E) stained images for the first time. Three-dimensional (3D) virtual H&E-stained images can be obtained at a scanning rate of 1 frame per second. Furthermore, we also demonstrate that this novel technique has been successfully applied in cancer diagnosis for the human central nervous system and breast, which proves that this new method will play a new unique and important role in intraoperative histology.","PeriodicalId":501709,"journal":{"name":"npj Imaging","volume":"3 1","pages":"12"},"PeriodicalIF":0.0,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12118749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144556385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The regulation of reporter transgene expression for diverse biological imaging applications. 多种生物成像应用中报告基因表达的调控。

npj Imaging Pub Date : 2025-03-04 DOI: 10.1038/s44303-025-00070-6

Alexandria S Battison, Joseph R Merrill, Jeremy C Borniger, Scott K Lyons

{"title":"The regulation of reporter transgene expression for diverse biological imaging applications.","authors":"Alexandria S Battison, Joseph R Merrill, Jeremy C Borniger, Scott K Lyons","doi":"10.1038/s44303-025-00070-6","DOIUrl":"10.1038/s44303-025-00070-6","url":null,"abstract":"A common strategy in biological research is to use the expression of a reporter transgene to non-destructively visualize or measure a biological parameter or process that is otherwise not visible or readily detectable. Genetic reporters have greatly impacted practically all fields of biological research, from visualizing biology in bacteria and microbes, through plant biology, to preclinical research in higher eukaryotes. Fundamentally, many reporter gene assays are reliant upon the expression of a relatively small number of transgenes (e.g., green fluorescent protein (GFP) or firefly luciferase (Fluc)), however, by regulating their expression in different ways, it is possible to generate enormous diversity in the specific aspects of biology measured. For the purposes of this review, rather than focus on the non-invasive imaging modalities or reporter transgenes themselves (recently reviewed1,2), we will instead discuss how reporter transgene expression can be manipulated to generate a huge variety of biological readouts, with specific emphasis in the fields of preclinical oncology and neuroscience, now coming together in the nascent field of cancer neuroscience.","PeriodicalId":501709,"journal":{"name":"npj Imaging","volume":"3 1","pages":"9"},"PeriodicalIF":0.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12118718/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144556397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The need for evidence-based, outcome-focused medical imaging research for cancer management. 癌症管理需要循证、注重结果的医学影像研究。

npj Imaging Pub Date : 2025-02-03 DOI: 10.1038/s44303-024-00067-7

Jiazheng Wang, Richard L Hesketh, John C Gore, Kevin M Brindle

引用次数: 0

Interventional real-time molecular MRI for targeting early myocardial injury in a pig model. 靶向猪模型早期心肌损伤的介入实时分子MRI。

npj Imaging Pub Date : 2025-02-03 DOI: 10.1038/s44303-025-00069-z

Timo Heidt, Simon Reiss, Julien Thielmann, Christian Weber, Alexander Maier, Thomas Lottner, Heidi R Cristina-Schmitz, Timon Bühler, Diana Chiang, Claus Jülicher, Carolin Wadle, Ingo Hilgendorf, Dennis Wolf, Gavin Tumlinson, Luis Hortells, Dirk Westermann, Michael Bock, Constantin von Zur Mühlen

{"title":"Interventional real-time molecular MRI for targeting early myocardial injury in a pig model.","authors":"Timo Heidt, Simon Reiss, Julien Thielmann, Christian Weber, Alexander Maier, Thomas Lottner, Heidi R Cristina-Schmitz, Timon Bühler, Diana Chiang, Claus Jülicher, Carolin Wadle, Ingo Hilgendorf, Dennis Wolf, Gavin Tumlinson, Luis Hortells, Dirk Westermann, Michael Bock, Constantin von Zur Mühlen","doi":"10.1038/s44303-025-00069-z","DOIUrl":"10.1038/s44303-025-00069-z","url":null,"abstract":"Myocardial ischemia induces tissue injury with subsequent inflammation and recruitment of immune cells. Besides myocardial tissue characterization, magnetic resonance imaging (MRI) allows for functional assessment using molecular imaging contrast agents. Here, we assessed ischemic cardiac lesions non-invasively directly after ischemia/reperfusion (I/R) in a porcine model by advanced MRI techniques and molecular imaging, targeting the cell adhesion molecule P-selectin functionalized with microparticles of iron oxide (MPIO). We used a closed-chest model of I/R by temporary coronary balloon-occlusion, real time 3T MRI-guided coronary injection of MPIO-based contrast agents, as well as injury, edema and iron-sensitive MRI. Within the first hours after I/R, we found T1 mapping to be most sensitive for tissue injury, with no changes in edema-sensitive MRI. Intriguingly, P-selectin MPIO contrast agent selectively enhanced the ischemic area in iron-sensitive MRI. In conclusion, this approach allows for sensitive detection of early myocardial inflammation beyond traditional edema-sensitive imaging.","PeriodicalId":501709,"journal":{"name":"npj Imaging","volume":"3 1","pages":"7"},"PeriodicalIF":0.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12118736/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144556388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0