npj Imaging最新文献_第3页

Rapid 3D imaging at cellular resolution for digital cytopathology with a multi-camera array scanner (MCAS) 利用多摄像头阵列扫描仪（MCAS）进行细胞分辨率的快速三维成像，用于数字细胞病理学研究

npj Imaging Pub Date : 2024-10-01 DOI: 10.1038/s44303-024-00042-2

Kanghyun Kim, Amey Chaware, Clare B. Cook, Shiqi Xu, Monica Abdelmalak, Colin Cooke, Kevin C. Zhou, Mark Harfouche, Paul Reamey, Veton Saliu, Jed Doman, Clay Dugo, Gregor Horstmeyer, Richard Davis, Ian Taylor-Cho, Wen-Chi Foo, Lucas Kreiss, Xiaoyin Sara Jiang, Roarke Horstmeyer

{"title":"Rapid 3D imaging at cellular resolution for digital cytopathology with a multi-camera array scanner (MCAS)","authors":"Kanghyun Kim, Amey Chaware, Clare B. Cook, Shiqi Xu, Monica Abdelmalak, Colin Cooke, Kevin C. Zhou, Mark Harfouche, Paul Reamey, Veton Saliu, Jed Doman, Clay Dugo, Gregor Horstmeyer, Richard Davis, Ian Taylor-Cho, Wen-Chi Foo, Lucas Kreiss, Xiaoyin Sara Jiang, Roarke Horstmeyer","doi":"10.1038/s44303-024-00042-2","DOIUrl":"10.1038/s44303-024-00042-2","url":null,"abstract":"Optical microscopy has long been the standard method for diagnosis in cytopathology. Whole slide scanners can image and digitize large sample areas automatically, but are slow, expensive and therefore not widely available. Clinical diagnosis of cytology specimens is especially challenging since these samples are both spread over large areas and thick, which requires 3D capture. Here, we introduce a new parallelized microscope for scanning thick specimens across extremely wide fields-of-view (54 × 72 mm2) at 1.2 and 0.6 μm resolutions, accompanied by machine learning software to rapidly assess these 16 gigapixel scans. This Multi-Camera Array Scanner (MCAS) comprises 48 micro-cameras closely arranged to simultaneously image different areas. By capturing 624 megapixels per snapshot, the MCAS is significantly faster than most conventional whole-slide scanners. We used this system to digitize entire cytology samples (scanning three entire slides in 3D in just several minutes) and demonstrate two machine learning techniques to assist pathologists: first, an adenocarcinoma detection model in lung specimens (0.73 recall); second, a slide-level classification model of lung smears (0.969 AUC).","PeriodicalId":501709,"journal":{"name":"npj Imaging","volume":" ","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s44303-024-00042-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142360081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Author Correction: [18F]FSPG-PET provides an early marker of radiotherapy response in head and neck squamous cell cancer 作者更正：[18F]FSPG-PET是头颈部鳞状细胞癌放疗反应的早期标志物

npj Imaging Pub Date : 2024-10-01 DOI: 10.1038/s44303-024-00049-9

Khrishanthne Sambasivan, Will E. Tyrrell, Rizwan Farooq, Jenasee Mynerich, Richard S. Edwards, Muhammet Tanc, Teresa Guerrero Urbano, Timothy H. Witney

引用次数: 0

KMnO4/Pb staining allows uranium free imaging of tissue architectures in low vacuum scanning electron microscopy KMnO4/Pb 染色技术可在低真空扫描电子显微镜下对组织结构进行无铀成像

npj Imaging Pub Date : 2024-10-01 DOI: 10.1038/s44303-024-00045-z

Akira Sawaguchi, Takeshi Kamimura, Kyoko Kitagawa, Yoko Nagashima, Nobuyasu Takahashi

{"title":"KMnO4/Pb staining allows uranium free imaging of tissue architectures in low vacuum scanning electron microscopy","authors":"Akira Sawaguchi, Takeshi Kamimura, Kyoko Kitagawa, Yoko Nagashima, Nobuyasu Takahashi","doi":"10.1038/s44303-024-00045-z","DOIUrl":"10.1038/s44303-024-00045-z","url":null,"abstract":"Scanning electron microscopy under low-vacuum conditions allows high-resolution imaging of complex cell/tissue architectures in nonconductive specimens. However, the conventional methods for metal staining of biological specimens require harmful uranium compounds, which hampers the applications of electron microscopy. Here, we introduce a uranium-free KMnO4/Pb metal staining protocol that allows multiscale imaging of extensive cell/tissue architectures to intensive subcellular ultrastructures. The obtained image contrast was equivalent to that of Ur/Pb staining and sufficient for ultrastructural observation, showing the fine processes of podocytes in the glomerulus, which were invisible by light microscopy. The stainability in the elastic tissue indicated that the distinct histochemical properties of KMnO4 oxidation led to Pb deposition and BSE signal enhancement superior to Ur staining. Elemental analysis clarified that the determinant of the backscattered electron signal intensity was the amount of Pb deposition enhanced by KMnO4 oxidation. This user-friendly method is anticipated to create a new approach for biomedical electron microscopy.","PeriodicalId":501709,"journal":{"name":"npj Imaging","volume":" ","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s44303-024-00045-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142360072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid full-color serial sectioning tomography with speckle illumination and ultraviolet excitation 利用斑点照明和紫外线激发进行快速全彩序列切片断层扫描

npj Imaging Pub Date : 2024-09-30 DOI: 10.1038/s44303-024-00040-4

Wentao Yu, Yan Zhang, Claudia T. K. Lo, Lei Kang, Terence T. W. Wong

{"title":"Rapid full-color serial sectioning tomography with speckle illumination and ultraviolet excitation","authors":"Wentao Yu, Yan Zhang, Claudia T. K. Lo, Lei Kang, Terence T. W. Wong","doi":"10.1038/s44303-024-00040-4","DOIUrl":"10.1038/s44303-024-00040-4","url":null,"abstract":"Three-dimensional (3D) high-resolution large-volume imaging has remained a challenge. Translational rapid ultraviolet-excited sectioning tomography (TRUST) achieves rapid and cost-effective whole-organ subcellular imaging through iterative optical scanning and mechanical sectioning. However, the axial resolution is limited by the mechanical sectioning thickness or the UV light penetration depth in tissue. Here, assisted with high-and-low-frequency (HiLo) microscopy (HiLoTRUST), the optical sectioning thickness has been reduced from tens of micrometers to ~5.8 µm. In addition, HiLoTRUST has attained a finer mechanical sectioning thickness (10–15 µm) compared to TRUST (50 µm). For high-content imaging as in TRUST, we employed two additional UV light-emitting diodes (LEDs) specifically for uniform illumination. The full-color imaging ability and improved axial resolution of HiLoTRUST have been validated by two-dimensional (2D)/3D imaging of various mouse organs and human lung cancer specimens. HiLoTRUST offers a cost-effective, full-color, and high-resolution 3D imaging approach, showing its great potential in 3D histology applications.","PeriodicalId":501709,"journal":{"name":"npj Imaging","volume":" ","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s44303-024-00040-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142329401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MALDI imaging combined with two-photon microscopy reveals local differences in the heterogeneity of colorectal cancer MALDI 成像结合双光子显微镜揭示结直肠癌异质性的局部差异

npj Imaging Pub Date : 2024-09-23 DOI: 10.1038/s44303-024-00041-3

Arora Bharti, Kulkarni Ajinkya, Markus M. Andrea, Ramos-Gomes Fernanda, Bohnenberger Hanibal, Ströbel Philipp, Alves Frauke, Klein Oliver

{"title":"MALDI imaging combined with two-photon microscopy reveals local differences in the heterogeneity of colorectal cancer","authors":"Arora Bharti, Kulkarni Ajinkya, Markus M. Andrea, Ramos-Gomes Fernanda, Bohnenberger Hanibal, Ströbel Philipp, Alves Frauke, Klein Oliver","doi":"10.1038/s44303-024-00041-3","DOIUrl":"10.1038/s44303-024-00041-3","url":null,"abstract":"Colorectal cancer (CRC) remains a leading cause of cancer-related mortality worldwide, accentuated by its heterogeneity and complex tumour microenvironment (TME). The role of TME on tumour pathophysiology is pivotal, especially the influence of components of the extracellular matrix (ECM), such as collagen. We introduce a novel multimodal imaging strategy to unravel the complex spatial heterogeneity of CRC by integrating the imaging features from two-photon laser scanning microscopy (2PLSM) and histology with proteomics signatures from matrix-assisted laser desorption ionization-mass spectrometry imaging (MALDI MSI). Our study is the first to correlate the structural coherence of collagen fibres and the nuclei distribution profile of tumour tissue with the peptide signatures, offering insights into the proteomic landscape of CRC within regions of high nuclei distribution (HND), as well as chaotic and organised regions of collagen. We use this approach to distinguish the patient tissues originating from left-sided colorectal cancer (LSCC) and from right-sided colorectal cancer (RSCC). This discriminative signature highlights the role of high nuclei distribution and collagen architecture in tumour progression. Complementary m/z values of several proteins associated to components of ECM, such as plectin, vinculin, vimentin, and myosin, have shown differentially intensity distributions between LSCC and RSCC. Our findings demonstrate the potential of combining structural information with peptide features to identify molecular signatures in different tumour regions and retrieve new insights into CRC pathophysiology.","PeriodicalId":501709,"journal":{"name":"npj Imaging","volume":" ","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s44303-024-00041-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142276661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnetic resonance reveals early lipid deposition in murine prediabetes as predictive marker for cardiovascular injury 磁共振发现小鼠糖尿病前期的早期脂质沉积是心血管损伤的预测标志物

npj Imaging Pub Date : 2024-09-23 DOI: 10.1038/s44303-024-00044-0

Katja Heller, Vera Flocke, Tamara Straub, Zhaoping Ding, Tanu Srivastava, Melissa Nowak, Florian Funk, Bodo Levkau, Joachim Schmitt, Maria Grandoch, Ulrich Flögel

{"title":"Magnetic resonance reveals early lipid deposition in murine prediabetes as predictive marker for cardiovascular injury","authors":"Katja Heller, Vera Flocke, Tamara Straub, Zhaoping Ding, Tanu Srivastava, Melissa Nowak, Florian Funk, Bodo Levkau, Joachim Schmitt, Maria Grandoch, Ulrich Flögel","doi":"10.1038/s44303-024-00044-0","DOIUrl":"10.1038/s44303-024-00044-0","url":null,"abstract":"People with diabetes have an increased cardiovascular risk and a poorer outcome after myocardial infarction (MI). However, the exact underlying mechanisms are still unclear, as is the question of which non-invasive measures could be used to predict the altered risk for the patient at early stages of the disease and adapt personalized treatment. Here, we used a holistic magnetic resonance approach to monitor longitudinally not only the main target heart, but also liver, peripheral/skeletal muscle, bone marrow, and hematopoiesis during disease development and subsequent MI. In prediabetic mice, we found a strong accumulation of lipids in all organs which preceded even a significant whole-body weight gain. Intramyocellular lipids (IMCLs) were most sensitive to reveal in vivo very early alterations in tissue properties during the prediabetic state. Subsequent induction of MI led to a persistent impairment of contractile function in septal/posterior segments of prediabetic hearts which correlated with their lipid load prior MI. At the same time, prediabetic cardiomyocytes exhibited sarcomere function at its limit resulting in overload and lower compensatory contractility of the healthy myocardium after MI. In summary, we identified IMCLs as very early marker in murine prediabetes and together with the cardiac lipid load as predictive for the functional outcome after MI.","PeriodicalId":501709,"journal":{"name":"npj Imaging","volume":" ","pages":"1-16"},"PeriodicalIF":0.0,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s44303-024-00044-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142276646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-invasive in vivo imaging of changes in Collagen III turnover in myocardial fibrosis 对心肌纤维化过程中胶原蛋白 III 更替变化的无创活体成像

npj Imaging Pub Date : 2024-09-17 DOI: 10.1038/s44303-024-00037-z

Nadia Chaher, Sara Lacerda, Giuseppe Digilio, Sergio Padovan, Ling Gao, Begoña Lavin, Rachele Stefania, Carlos Velasco, Gastão Cruz, Claudia Prieto, René M. Botnar, Alkystis Phinikaridou

{"title":"Non-invasive in vivo imaging of changes in Collagen III turnover in myocardial fibrosis","authors":"Nadia Chaher, Sara Lacerda, Giuseppe Digilio, Sergio Padovan, Ling Gao, Begoña Lavin, Rachele Stefania, Carlos Velasco, Gastão Cruz, Claudia Prieto, René M. Botnar, Alkystis Phinikaridou","doi":"10.1038/s44303-024-00037-z","DOIUrl":"10.1038/s44303-024-00037-z","url":null,"abstract":"Heart failure (HF) affects 64 million people globally with enormous societal and healthcare costs. Myocardial fibrosis, characterised by changes in collagen content drives HF. Despite evidence that collagen type III (COL3) content changes during myocardial fibrosis, in vivo imaging of COL3 has not been achieved. Here, we discovered the first imaging probe that binds to COL3 with high affinity and specificity, by screening candidate peptide-based probes. Characterisation of the probe showed favourable magnetic and biodistribution properties. The probe’s potential for in vivo molecular cardiac magnetic resonance imaging was evaluated in a murine model of myocardial infarction. Using the new probe, we were able to map and quantify, previously undetectable, spatiotemporal changes in COL3 after myocardial infarction and monitor response to treatment. This innovative probe provides a promising tool to non-invasively study the unexplored roles of COL3 in cardiac fibrosis and other cardiovascular conditions marked by changes in COL3.","PeriodicalId":501709,"journal":{"name":"npj Imaging","volume":" ","pages":"1-15"},"PeriodicalIF":0.0,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s44303-024-00037-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142236106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Real-time tracking of the Bragg peak during proton therapy via 3D protoacoustic Imaging in a clinical scenario 在临床场景中通过三维原声成像对质子治疗过程中的布拉格峰进行实时跟踪

npj Imaging Pub Date : 2024-09-17 DOI: 10.1038/s44303-024-00039-x

Siqi Wang, Gilberto Gonzalez, Leshan Sun, Yifei Xu, Prabodh Pandey, Yong Chen, Shawn (Liangzhong) Xiang

{"title":"Real-time tracking of the Bragg peak during proton therapy via 3D protoacoustic Imaging in a clinical scenario","authors":"Siqi Wang, Gilberto Gonzalez, Leshan Sun, Yifei Xu, Prabodh Pandey, Yong Chen, Shawn (Liangzhong) Xiang","doi":"10.1038/s44303-024-00039-x","DOIUrl":"10.1038/s44303-024-00039-x","url":null,"abstract":"Proton radiotherapy favored over X-ray photon therapy due to its reduced radiation exposure to surrounding healthy tissues, is highly dependent on the accurate positioning of the Bragg peak. Existing methods like PET and prompt gamma imaging to localize Bragg peak face challenges of low precision and high complexity. Here we introduce a 3D protoacoustic imaging with a 2D matrix array of 256 ultrasound transducers compatible with 256 parallel data acquisition channels provides real-time imaging capability (up to 75 frames per second with 10 averages), achieving high precision (5 mm/5% Gamma index shows accuracy better than 95.73%) at depths of tens of centimeters. We have successfully implemented this method in liver treatment with 5 pencil beam scanning and in prostate cancer treatment on a human torso phantom using a clinical proton machine. This demonstrates its capability to accurately identify the Bragg peak in practical clinical scenarios. It paves the way for adaptive radiotherapy with real-time feedback, potentially revolutionizing radiotherapy by enabling closed-loop treatment for improved patient outcomes.","PeriodicalId":501709,"journal":{"name":"npj Imaging","volume":" ","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s44303-024-00039-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142236104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of [18F]FDG-PET/CT in Staphylococcus aureus bacteremia: A clinical perspective 18F]FDG-PET/CT 在金黄色葡萄球菌菌血症中的作用：临床视角

npj Imaging Pub Date : 2024-09-13 DOI: 10.1038/s44303-024-00036-0

Ilse J. E. Kouijzer, Nesrin Ghanem-Zoubi

引用次数: 0

Quantitative microbiology with widefield microscopy: navigating optical artefacts for accurate interpretations 使用宽视野显微镜进行微生物定量分析：利用光学伪影进行准确解释

npj Imaging Pub Date : 2024-09-02 DOI: 10.1038/s44303-024-00024-4

Georgeos Hardo, Ruizhe Li, Somenath Bakshi

{"title":"Quantitative microbiology with widefield microscopy: navigating optical artefacts for accurate interpretations","authors":"Georgeos Hardo, Ruizhe Li, Somenath Bakshi","doi":"10.1038/s44303-024-00024-4","DOIUrl":"10.1038/s44303-024-00024-4","url":null,"abstract":"Time-resolved live-cell imaging using widefield microscopy is instrumental in quantitative microbiology research. It allows researchers to track and measure the size, shape, and content of individual microbial cells over time. However, the small size of microbial cells poses a significant challenge in interpreting image data, as their dimensions approache that of the microscope’s depth of field, and they begin to experience significant diffraction effects. As a result, 2D widefield images of microbial cells contain projected 3D information, blurred by the 3D point spread function. In this study, we employed simulations and targeted experiments to investigate the impact of diffraction and projection on our ability to quantify the size and content of microbial cells from 2D microscopic images. This study points to some new and often unconsidered artefacts resulting from the interplay of projection and diffraction effects, within the context of quantitative microbiology. These artefacts introduce substantial errors and biases in size, fluorescence quantification, and even single-molecule counting, making the elimination of these errors a complex task. Awareness of these artefacts is crucial for designing strategies to accurately interpret micrographs of microbes. To address this, we present new experimental designs and machine learning-based analysis methods that account for these effects, resulting in accurate quantification of microbiological processes.","PeriodicalId":501709,"journal":{"name":"npj Imaging","volume":" ","pages":"1-16"},"PeriodicalIF":0.0,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s44303-024-00024-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142123432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0