npj Imaging最新文献_第2页

Stochastically structured illumination microscopy scan less super resolution imaging 随机结构照明显微镜扫描少超分辨率成像

npj Imaging Pub Date : 2024-11-06 DOI: 10.1038/s44303-024-00047-x

Denzel Fusco, Emmanouil Xypakis, Ylenia Gigante, Lorenza Mautone, Silvia Di Angelantonio, Giorgia Ponsi, Giancarlo Ruocco, Marco Leonetti

{"title":"Stochastically structured illumination microscopy scan less super resolution imaging","authors":"Denzel Fusco, Emmanouil Xypakis, Ylenia Gigante, Lorenza Mautone, Silvia Di Angelantonio, Giorgia Ponsi, Giancarlo Ruocco, Marco Leonetti","doi":"10.1038/s44303-024-00047-x","DOIUrl":"10.1038/s44303-024-00047-x","url":null,"abstract":"In super-resolution, a varying illumination image stack is required. This enriched dataset typically necessitates precise mechanical control and micron-scale optical alignment and repeatability. Here, we introduce a novel methodology for super-resolution microscopy called stochastically structured illumination microscopy (S2IM), which bypasses the need for illumination control exploiting instead the random, uncontrolled movement of the target object. We tested our methodology within the clinically relevant ophthalmoscopic setting, harnessing the inherent saccadic motion of the eye to induce stochastic displacement of the illumination pattern on the retina. We opted to avoid human subjects by utilizing a phantom eye model featuring a retina composed of human induced pluripotent stem cells (iPSC) retinal neurons and replicating the ocular saccadic movements by custom actuators. Our findings demonstrate that S2IM unlocks scan-less super-resolution with a resolution enhancement of 1.91, with promising prospects also beyond ophthalmoscopy applications such as active matter or atmospheric/astronomical observation.","PeriodicalId":501709,"journal":{"name":"npj Imaging","volume":" ","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s44303-024-00047-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142588291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of photobleaching on quantitative, spatio-temporal, super-resolution imaging of mitochondria in live C. elegans larvae 光漂白对线粒体定量、时空、超分辨率活体线粒体成像的影响

npj Imaging Pub Date : 2024-11-06 DOI: 10.1038/s44303-024-00043-1

Segos Ioannis, Van Eeckhoven Jens, Greig Alan, Redd Michael, Thrasivoulou Christopher, Conradt Barbara

{"title":"Impact of photobleaching on quantitative, spatio-temporal, super-resolution imaging of mitochondria in live C. elegans larvae","authors":"Segos Ioannis, Van Eeckhoven Jens, Greig Alan, Redd Michael, Thrasivoulou Christopher, Conradt Barbara","doi":"10.1038/s44303-024-00043-1","DOIUrl":"10.1038/s44303-024-00043-1","url":null,"abstract":"Super-resolution (SR) 3D rendering allows superior quantitative analysis of intracellular structures but has largely been limited to fixed or ex vivo samples. Here we developed a method to perform SR live imaging of mitochondria during post-embryonic development of C. elegans larvae. Our workflow includes the drug-free mechanical immobilisation of animals using polystyrene nanobeads, which has previously not been used for in vivo SR imaging. Based on the alignment of moving objects and global threshold-based image segmentation, our method enables an efficient 3D reconstruction of individual mitochondria. We demonstrate for the first time that the frequency distribution of fluorescence intensities is not affected by photobleaching, and that global thresholding alone enables the quantitative comparison of mitochondria along timeseries. Our composite approach significantly improves the study of biological structures and processes in SR during C. elegans post-embryonic development. Furthermore, the discovery that image segmentation does not require any prior correction against photobleaching, a fundamental problem in fluorescence microscopy, will impact experimental strategies aimed at quantitatively studying the dynamics of organelles and other intracellular compartments in any biological system.","PeriodicalId":501709,"journal":{"name":"npj Imaging","volume":" ","pages":"1-15"},"PeriodicalIF":0.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s44303-024-00043-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142588277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of dietary zinc on stimulated zinc secretion MRI in the healthy and malignant mouse prostate 膳食锌对健康和恶性小鼠前列腺锌分泌磁共振成像的影响

npj Imaging Pub Date : 2024-11-06 DOI: 10.1038/s44303-024-00051-1

Veronica Clavijo Jordan, André F. Martins, Erica Dao, Kalotina Geraki, Sara Chirayil, Xiaodong Wen, Pooyan Khalighinejad, Daniel Parrott, Xiaojing Wang, Patricia Gonzalez Pagan, Neil Rofsky, Michael Farquharson, A. Dean Sherry

{"title":"Impact of dietary zinc on stimulated zinc secretion MRI in the healthy and malignant mouse prostate","authors":"Veronica Clavijo Jordan, André F. Martins, Erica Dao, Kalotina Geraki, Sara Chirayil, Xiaodong Wen, Pooyan Khalighinejad, Daniel Parrott, Xiaojing Wang, Patricia Gonzalez Pagan, Neil Rofsky, Michael Farquharson, A. Dean Sherry","doi":"10.1038/s44303-024-00051-1","DOIUrl":"10.1038/s44303-024-00051-1","url":null,"abstract":"Previous studies have shown that the zinc-responsive MRI probe, GdL1, can distinguish healthy versus malignant prostate tissues based upon differences in zinc content and secretion. In this study, mice were fed chow containing low, normal, or high zinc content for 3 weeks before imaging glucose stimulated zinc secretion (GSZS) by MRI. The distribution of zinc in prostate tissue in these three groups was imaged by synchrotron radiation X-ray fluorescence (SR-XRF). A zinc deficiency caused systemic and organ-level dysregulation, weight loss, and altered zinc bioavailability. Zinc efflux from the prostate increased in parallel to dietary zinc in healthy mice but not in TRAMP mice, consistent with a lowered capacity to store dietary zinc in malignant cells. This differential zinc efflux suggests that a dietary supplement of zinc prior to a GSZS study may enhance image contrast between healthy and malignant prostate tissue, thereby improving the accuracy of prostate cancer detection in man.","PeriodicalId":501709,"journal":{"name":"npj Imaging","volume":" ","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s44303-024-00051-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142588292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deep tissue photoacoustic imaging with light and sound 用光和声音进行深层组织光声成像

npj Imaging Pub Date : 2024-11-06 DOI: 10.1038/s44303-024-00048-w

Luca Menozzi, Junjie Yao

引用次数: 0

Publisher Correction: Magnetic resonance reveals early lipid deposition in murine prediabetes as predictive marker for cardiovascular injury 出版商更正：磁共振发现小鼠糖尿病前期的早期脂质沉积是心血管损伤的预测标志物

npj Imaging Pub Date : 2024-10-11 DOI: 10.1038/s44303-024-00050-2

Katja Heller, Vera Flocke, Tamara Straub, Zhaoping Ding, Tanu Srivastava, Melissa Nowak, Florian Funk, Bodo Levkau, Joachim Schmitt, Maria Grandoch, Ulrich Flögel

引用次数: 0

Label-free live cell recognition and tracking for biological discoveries and translational applications 用于生物发现和转化应用的无标签活细胞识别与追踪技术

npj Imaging Pub Date : 2024-10-07 DOI: 10.1038/s44303-024-00046-y

Biqi Chen, Zi Yin, Billy Wai-Lung Ng, Dan Michelle Wang, Rocky S. Tuan, Ryoma Bise, Dai Fei Elmer Ker

引用次数: 0

Rapid 3D imaging at cellular resolution for digital cytopathology with a multi-camera array scanner (MCAS) 利用多摄像头阵列扫描仪（MCAS）进行细胞分辨率的快速三维成像，用于数字细胞病理学研究

npj Imaging Pub Date : 2024-10-01 DOI: 10.1038/s44303-024-00042-2

Kanghyun Kim, Amey Chaware, Clare B. Cook, Shiqi Xu, Monica Abdelmalak, Colin Cooke, Kevin C. Zhou, Mark Harfouche, Paul Reamey, Veton Saliu, Jed Doman, Clay Dugo, Gregor Horstmeyer, Richard Davis, Ian Taylor-Cho, Wen-Chi Foo, Lucas Kreiss, Xiaoyin Sara Jiang, Roarke Horstmeyer

{"title":"Rapid 3D imaging at cellular resolution for digital cytopathology with a multi-camera array scanner (MCAS)","authors":"Kanghyun Kim, Amey Chaware, Clare B. Cook, Shiqi Xu, Monica Abdelmalak, Colin Cooke, Kevin C. Zhou, Mark Harfouche, Paul Reamey, Veton Saliu, Jed Doman, Clay Dugo, Gregor Horstmeyer, Richard Davis, Ian Taylor-Cho, Wen-Chi Foo, Lucas Kreiss, Xiaoyin Sara Jiang, Roarke Horstmeyer","doi":"10.1038/s44303-024-00042-2","DOIUrl":"10.1038/s44303-024-00042-2","url":null,"abstract":"Optical microscopy has long been the standard method for diagnosis in cytopathology. Whole slide scanners can image and digitize large sample areas automatically, but are slow, expensive and therefore not widely available. Clinical diagnosis of cytology specimens is especially challenging since these samples are both spread over large areas and thick, which requires 3D capture. Here, we introduce a new parallelized microscope for scanning thick specimens across extremely wide fields-of-view (54 × 72 mm2) at 1.2 and 0.6 μm resolutions, accompanied by machine learning software to rapidly assess these 16 gigapixel scans. This Multi-Camera Array Scanner (MCAS) comprises 48 micro-cameras closely arranged to simultaneously image different areas. By capturing 624 megapixels per snapshot, the MCAS is significantly faster than most conventional whole-slide scanners. We used this system to digitize entire cytology samples (scanning three entire slides in 3D in just several minutes) and demonstrate two machine learning techniques to assist pathologists: first, an adenocarcinoma detection model in lung specimens (0.73 recall); second, a slide-level classification model of lung smears (0.969 AUC).","PeriodicalId":501709,"journal":{"name":"npj Imaging","volume":" ","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s44303-024-00042-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142360081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Author Correction: [18F]FSPG-PET provides an early marker of radiotherapy response in head and neck squamous cell cancer 作者更正：[18F]FSPG-PET是头颈部鳞状细胞癌放疗反应的早期标志物

npj Imaging Pub Date : 2024-10-01 DOI: 10.1038/s44303-024-00049-9

Khrishanthne Sambasivan, Will E. Tyrrell, Rizwan Farooq, Jenasee Mynerich, Richard S. Edwards, Muhammet Tanc, Teresa Guerrero Urbano, Timothy H. Witney

引用次数: 0

KMnO4/Pb staining allows uranium free imaging of tissue architectures in low vacuum scanning electron microscopy KMnO4/Pb 染色技术可在低真空扫描电子显微镜下对组织结构进行无铀成像

npj Imaging Pub Date : 2024-10-01 DOI: 10.1038/s44303-024-00045-z

Akira Sawaguchi, Takeshi Kamimura, Kyoko Kitagawa, Yoko Nagashima, Nobuyasu Takahashi

{"title":"KMnO4/Pb staining allows uranium free imaging of tissue architectures in low vacuum scanning electron microscopy","authors":"Akira Sawaguchi, Takeshi Kamimura, Kyoko Kitagawa, Yoko Nagashima, Nobuyasu Takahashi","doi":"10.1038/s44303-024-00045-z","DOIUrl":"10.1038/s44303-024-00045-z","url":null,"abstract":"Scanning electron microscopy under low-vacuum conditions allows high-resolution imaging of complex cell/tissue architectures in nonconductive specimens. However, the conventional methods for metal staining of biological specimens require harmful uranium compounds, which hampers the applications of electron microscopy. Here, we introduce a uranium-free KMnO4/Pb metal staining protocol that allows multiscale imaging of extensive cell/tissue architectures to intensive subcellular ultrastructures. The obtained image contrast was equivalent to that of Ur/Pb staining and sufficient for ultrastructural observation, showing the fine processes of podocytes in the glomerulus, which were invisible by light microscopy. The stainability in the elastic tissue indicated that the distinct histochemical properties of KMnO4 oxidation led to Pb deposition and BSE signal enhancement superior to Ur staining. Elemental analysis clarified that the determinant of the backscattered electron signal intensity was the amount of Pb deposition enhanced by KMnO4 oxidation. This user-friendly method is anticipated to create a new approach for biomedical electron microscopy.","PeriodicalId":501709,"journal":{"name":"npj Imaging","volume":" ","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s44303-024-00045-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142360072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid full-color serial sectioning tomography with speckle illumination and ultraviolet excitation 利用斑点照明和紫外线激发进行快速全彩序列切片断层扫描

npj Imaging Pub Date : 2024-09-30 DOI: 10.1038/s44303-024-00040-4

Wentao Yu, Yan Zhang, Claudia T. K. Lo, Lei Kang, Terence T. W. Wong

{"title":"Rapid full-color serial sectioning tomography with speckle illumination and ultraviolet excitation","authors":"Wentao Yu, Yan Zhang, Claudia T. K. Lo, Lei Kang, Terence T. W. Wong","doi":"10.1038/s44303-024-00040-4","DOIUrl":"10.1038/s44303-024-00040-4","url":null,"abstract":"Three-dimensional (3D) high-resolution large-volume imaging has remained a challenge. Translational rapid ultraviolet-excited sectioning tomography (TRUST) achieves rapid and cost-effective whole-organ subcellular imaging through iterative optical scanning and mechanical sectioning. However, the axial resolution is limited by the mechanical sectioning thickness or the UV light penetration depth in tissue. Here, assisted with high-and-low-frequency (HiLo) microscopy (HiLoTRUST), the optical sectioning thickness has been reduced from tens of micrometers to ~5.8 µm. In addition, HiLoTRUST has attained a finer mechanical sectioning thickness (10–15 µm) compared to TRUST (50 µm). For high-content imaging as in TRUST, we employed two additional UV light-emitting diodes (LEDs) specifically for uniform illumination. The full-color imaging ability and improved axial resolution of HiLoTRUST have been validated by two-dimensional (2D)/3D imaging of various mouse organs and human lung cancer specimens. HiLoTRUST offers a cost-effective, full-color, and high-resolution 3D imaging approach, showing its great potential in 3D histology applications.","PeriodicalId":501709,"journal":{"name":"npj Imaging","volume":" ","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s44303-024-00040-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142329401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0