npj Imaging Pub Date : 2026-05-08 DOI: 10.1038/s44303-026-00148-9

Xusan Yang, Siyang Liu, Fei Xia, Meiqi Wu, Chris Xu, Steven G Adie

引用次数: 0

[⁶⁴Cu]Cu-DOTA-TYPE7: a targeted PET radiotracer for imaging EphA2+ tumors. [64Cu]Cu-DOTA-TYPE7: EphA2+肿瘤的靶向PET示踪剂

npj Imaging Pub Date : 2026-05-04 DOI: 10.1038/s44303-026-00168-5

Alexis M Sanwick, Daiane S Alves, Katherine N Haugh, Francisco N Barrera, Ivis F Chaple

{"title":"[64Cu]Cu-DOTA-TYPE7: a targeted PET radiotracer for imaging EphA2+ tumors.","authors":"Alexis M Sanwick, Daiane S Alves, Katherine N Haugh, Francisco N Barrera, Ivis F Chaple","doi":"10.1038/s44303-026-00168-5","DOIUrl":"https://doi.org/10.1038/s44303-026-00168-5","url":null,"abstract":"The EphA2 receptor tyrosine kinase has been implicated in cellular transformation and malignancy and is frequently overexpressed in a wide range of cancers, including those of the breast, bladder, esophagus, pancreas, prostate, lung, and colon. Due to its oncogenic relevance, EphA2 represents an attractive molecular target for precision imaging and therapy. This study reports the development of a novel 64Cu-labeled peptide, TYPE7, for PET imaging of EphA2 expressing tumors. TYPE7 is a soluble peptide that integrates into the cell membrane where it targets EphA2 to induce receptor oligomerization and phosphorylation. Here, we describe the synthesis and characterization of [64Cu]Cu-DOTA-TYPE7 and evaluate its in vitro binding properties and in vivo performance in an EphA2+ tumor model. [64Cu]Cu-DOTA-TYPE7 was synthesized under mild reaction conditions, exhibiting radiochemical yields > 95%. The results obtained from the cellular binding assays using PANC-1 (EphA2 + ) and HEK-293T (EphA2-) cell lines support EphA2 specific binding. PET imaging enabled clear visualization of EphA2+ tumors, with peak uptake observed 4 h post injection, consistent with ex vivo biodistribution results. Together, these findings establish [64Cu]Cu-DOTA-TYPE7 as a promising PET tracer for imaging EphA2-expressing diseases and provides a foundation for future development of EphA2-targeted theranostic applications.","PeriodicalId":501709,"journal":{"name":"npj Imaging","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13139581/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147848545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-invasive measurement of accelerated gastrointestinal transit in pediatric patients using Contrast-enhanced Multispectral optoacoustic tomography. 使用对比度增强多光谱光声断层扫描无创测量儿科患者胃肠加速传输。

npj Imaging Pub Date : 2026-05-04 DOI: 10.1038/s44303-026-00169-4

Luisa Caselitz, Merle Claßen, Adrian Bühler, Lars-Philip Paulus, Henriette Grieshaber-Bouyer Mandelbaum, Emmanuel Nedoschill, Joachim Wölfle, André Hörning, Ferdinand Knieling, Adrian Philip Regensburger, Felix Wachter

{"title":"Non-invasive measurement of accelerated gastrointestinal transit in pediatric patients using Contrast-enhanced Multispectral optoacoustic tomography.","authors":"Luisa Caselitz, Merle Claßen, Adrian Bühler, Lars-Philip Paulus, Henriette Grieshaber-Bouyer Mandelbaum, Emmanuel Nedoschill, Joachim Wölfle, André Hörning, Ferdinand Knieling, Adrian Philip Regensburger, Felix Wachter","doi":"10.1038/s44303-026-00169-4","DOIUrl":"https://doi.org/10.1038/s44303-026-00169-4","url":null,"abstract":"Functional intestinal disorders are common; however, current imaging modalities are limited. Contrast-enhanced Multispectral optoacoustic tomography (CE-MSOT) represents a novel, non-invasive, and radiation-free imaging technique that enables assessment of gastrointestinal transit time through the use of orally administered contrast agents. This could aid in symptom evaluation and diagnostic clarification in bowel disorders. A clinical pilot-study was conducted (Registry: ClinicalTrials.gov, TRN: NCT06617364, Registration date: 24 September 2024) involving 10 pediatric patients presenting with gastrointestinal complaints undergoing a lactose hydrogen breath test. CE-MSOT was performed following the oral administration of Indocyanine Green (ICG) as a contrast agent. In the terminal ileum and sigmoid colon multispectral optoacoustic tomography (MSOT) signals were spectrally unmixed to isolate ICG-specific signals. The appearance of ICG in these segments was used to estimate gastrointestinal transit time. ICG signals were detected in the terminal ileum as early as 13 min after oral administration (median: 80 min), and in the sigmoid colon as early as 41 min (median: 125 min).","PeriodicalId":501709,"journal":{"name":"npj Imaging","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13139493/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147848626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Homogeneous image-based digital immunoassays with high error tolerance. 具有高容错性的均匀图像数字免疫分析。

npj Imaging Pub Date : 2026-05-04 DOI: 10.1038/s44303-026-00164-9

Darren B McAffee, Qiang Hu, Assame Arnob, Hung-Jen Wu, Jay T Groves

{"title":"Homogeneous image-based digital immunoassays with high error tolerance.","authors":"Darren B McAffee, Qiang Hu, Assame Arnob, Hung-Jen Wu, Jay T Groves","doi":"10.1038/s44303-026-00164-9","DOIUrl":"https://doi.org/10.1038/s44303-026-00164-9","url":null,"abstract":"There is a significant global health need to translate more in vitro diagnostic tests from clinical laboratories to field-based applications, including point-of-care and self-administered test formats. These applications typically require smaller sample sizes, limit sample processing and measurement capabilities, and introduce greater handling variability. Error tolerance is one of the most critical factors for successful field-based assay design. Here, we examine machine-learning (ML) strategies to enhance the error tolerance of image-based nanoparticle immunoassays. Random dispersions of nanoparticles were imaged in microliter sample volumes, and images were processed to determine analyte concentrations based on nanoparticle appearance. Assay performance was characterized using two common blood analytes: C-reactive protein and anti-SARS-CoV-2 IgG. We compare the results from conventional image analysis, a hybrid ML-conventional approach based on pixel segmentation, and end-to-end image regression using a targeted regularization strategy. Using serum samples from SARS-CoV-2 positive individuals, the segmentation-based approach enabled binary classification with 96% specificity and 90% sensitivity, matching seroconversion rates. The end-to-end regression model achieved superior quantitative performance (5.2 ng/mL), approaching ELISA-level detection range (0.01-10 ng/mL, depending on capture antibody affinity) in a single 30 min workflow without sample preprocessing. The limit of detection for digital molecular assays is not fixed, and we perform a theoretical analysis showing how adjusting particle counts and polydispersity can achieve arbitrary sensitivity down to the Poisson limit. Training images for the full image regression approach required only a single label-the analyte concentration-eliminating labor-intensive pixel-level labeling. Ultimately, the image-based readouts significantly improved dynamic range, sensitivity, and reproducibility over conventional readouts.","PeriodicalId":501709,"journal":{"name":"npj Imaging","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13139397/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147848585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-contrast laser endoscopy for in vivo gastrointestinal imaging. 多对比激光内窥镜用于体内胃肠道成像。

npj Imaging Pub Date : 2026-05-04 DOI: 10.1038/s44303-026-00161-y

Taylor L Bobrow, Mayank Golhar, Suchapa Arayakarnkul, Anthony A Song, Saowanee Ngamruengphong, Nicholas J Durr

{"title":"Multi-contrast laser endoscopy for in vivo gastrointestinal imaging.","authors":"Taylor L Bobrow, Mayank Golhar, Suchapa Arayakarnkul, Anthony A Song, Saowanee Ngamruengphong, Nicholas J Durr","doi":"10.1038/s44303-026-00161-y","DOIUrl":"https://doi.org/10.1038/s44303-026-00161-y","url":null,"abstract":"White light endoscopy is the clinical gold standard for detecting diseases in the gastrointestinal tract. Most applications involve identifying visual abnormalities in tissue color, texture, and shape. Unfortunately, the contrast of these features is often subtle, causing many clinically relevant cases to go undetected. To overcome this challenge, we introduce Multi-contrast Laser Endoscopy (MLE): a platform for widefield clinical imaging with rapidly tunable spectral, coherent, and directional illumination. We demonstrate three capabilities of MLE: enhancing tissue chromophore contrast with multispectral diffuse reflectance, quantifying blood flow using laser speckle contrast imaging, and characterizing mucosal topography using photometric stereo. We validate MLE with benchtop models, then demonstrate MLE in vivo during clinical colonoscopies. MLE images from 31 polyps demonstrate an approximate three-fold improvement in contrast and a five-fold improvement in color difference compared to white light and narrow band imaging. With the ability to reveal multiple complementary types of tissue contrast while seamlessly integrating into the clinical environment, MLE shows promise as an investigative tool to improve gastrointestinal imaging.","PeriodicalId":501709,"journal":{"name":"npj Imaging","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13139481/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147848640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of in vivo 7T magnetic resonance neuroimaging via flexible metasurfaces. 通过柔性超表面增强体内7T磁共振神经成像。

npj Imaging Pub Date : 2026-04-16 DOI: 10.1038/s44303-026-00162-x

Paul S Jacobs, Neil Wilson, Wyger Brink, John Detre, Mark Elliott, Ravinder Reddy

引用次数: 0

[3-¹¹C]Pyruvate PET detects alterations in cardiac pyruvate metabolism induced by doxorubicin chemotherapy. [3-11C]丙酮酸PET检测多柔比星化疗引起的心脏丙酮酸代谢改变。

npj Imaging Pub Date : 2026-04-16 DOI: 10.1038/s44303-026-00165-8

Chul-Hee Lee, Thomas Ruan, Shuvra Debnath, Anja S Wacker, Grace Figlioli, John W Babich, Sadek A Nehmeh, Kayvan R Keshari, James M Kelly

{"title":"[3-11C]Pyruvate PET detects alterations in cardiac pyruvate metabolism induced by doxorubicin chemotherapy.","authors":"Chul-Hee Lee, Thomas Ruan, Shuvra Debnath, Anja S Wacker, Grace Figlioli, John W Babich, Sadek A Nehmeh, Kayvan R Keshari, James M Kelly","doi":"10.1038/s44303-026-00165-8","DOIUrl":"10.1038/s44303-026-00165-8","url":null,"abstract":"Changes in cardiac metabolism typically precede cardiac dysfunction and therefore represent an important target for diagnosis and treatment designed to prevent progression to heart failure, a leading cause of death. Profound changes in pyruvate metabolism, including reduced expression of the mitochondrial pyruvate carrier (MPC), are increasingly recognized as early maladaptive alterations in cardiomyopathies, but no methods currently exist to determine MPC expression in vivo. We exposed mice to doxorubicin (DOX), an anthracycline chemotherapeutic known to perturb pyruvate metabolism, and demonstrated that cardiac tissue levels of MPC decrease within 4 weeks of initial DOX exposure. Using a combination of stable isotope tracing metabolomics, hyperpolarized [1-13C]pyruvate magnetic resonance imaging (MRI), and [3-11C]pyruvate positron emission tomography (PET), we found that loss of MPC and monocarboxylate transporter 1 (MCT1) resulted in decreased utilization of pyruvate for mitochondrial oxidative metabolism and resulted in decreased cardiac carbon-11 clearance. Despite recovery of expression levels of pyruvate transporters, including MPC, 16 weeks after initial DOX exposure, cardiac carbon-11 clearance still trends towards differences between control mice and the mice exposed to this chemotherapeutic. [3-11C]Pyruvate PET is therefore a promising approach to imaging cardiac pyruvate transport with potential applications to the identification of early maladaptive changes in pyruvate metabolism and monitoring response to therapy.","PeriodicalId":501709,"journal":{"name":"npj Imaging","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13087250/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147701446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioimage analysis for multiplexed FUCCI acquisitions powered by deep learning. 基于深度学习的多路fuci采集生物图像分析。

npj Imaging Pub Date : 2026-04-14 DOI: 10.1038/s44303-026-00159-6

J Zimmermann, M Pezzotti, E Torchia, A Enrico, S Rigolli, M Di Sante, F S Pasqualini

引用次数: 0

Author Correction: Label-free whole slide virtual multi-staining using dual-excitation photon absorption remote sensing microscopy. 作者更正：使用双激发光子吸收遥感显微镜进行无标签全片虚拟多重染色。

npj Imaging Pub Date : 2026-04-10 DOI: 10.1038/s44303-026-00163-w

James E D Tweel, Benjamin R Ecclestone, James A Tummon Simmons, Parsin Haji Reza

引用次数: 0

MRI of combination immunotherapy in an epithelial ovarian cancer preclinical model. 联合免疫治疗在上皮性卵巢癌临床前模型中的MRI表现。

npj Imaging Pub Date : 2026-04-09 DOI: 10.1038/s44303-026-00157-8

Jessica T Gosse, Caitrin Sobey Skelton, Marie-Laurence Tremblay, Hailey Wyatt, Victoria Gonzalez, Bassel Dawod, Andrea Nuschke, Christa Davis, Brennan Dirk, Ava Vila-Leahey, Alecia Mackay, Kim Bobbitt, Andrea West, Barbara Vanderhyden, Genevieve Weir, Alexandra Merkx-Jacques, Marianne Stanford, Olga Hrytsenko, Kimberly D Brewer

{"title":"MRI of combination immunotherapy in an epithelial ovarian cancer preclinical model.","authors":"Jessica T Gosse, Caitrin Sobey Skelton, Marie-Laurence Tremblay, Hailey Wyatt, Victoria Gonzalez, Bassel Dawod, Andrea Nuschke, Christa Davis, Brennan Dirk, Ava Vila-Leahey, Alecia Mackay, Kim Bobbitt, Andrea West, Barbara Vanderhyden, Genevieve Weir, Alexandra Merkx-Jacques, Marianne Stanford, Olga Hrytsenko, Kimberly D Brewer","doi":"10.1038/s44303-026-00157-8","DOIUrl":"10.1038/s44303-026-00157-8","url":null,"abstract":"Immunotherapies such as checkpoint inhibitors (i.e. anti-PD-1) and peptide-based therapies (DPX-Survivac) have strong potential for treatment of epithelial ovarian cancer, the most lethal gynecological malignancy. Magnetic resonance imaging (MRI) can be used to track tumor growth and iron-labeled immune cells longitudinally at the individual level. We studied MRI immune cell tracking in a murine model of ovarian cancer using a clinically relevant treatment combination of DPX-Survivac, anti-PD-1, and an intermittent low dose of Cyclophosphamide (CPA). HHD-DR1 mice were orthotopically implanted with mouse ovarian surface epithelial (MOSE) cancer cells. Myeloid and CD8+ cells were isolated from matched donor mice, labeled with superparamagnetic iron oxide (SPIO) and were scanned using MRI on days 42, 49 and 56. Tumor volumes in the treatment group as measured by MRI were significantly lower than in the control group (p < 0.01). The density of SPIO-labeled myeloid and CD8+ T cells in tumors was higher in the treatment group than in the control group. This study provides insights into how MRI can be used in concert with biological assays to study how immunotherapy and chemotherapy combinations exert their antitumor effects.","PeriodicalId":501709,"journal":{"name":"npj Imaging","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13066022/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147647915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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