npj Imaging最新文献

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Fast histological assessment of adipose tissue inflammation by label-free mid-infrared optoacoustic microscopy 利用无标记中红外光声显微镜对脂肪组织炎症进行快速组织学评估

npj Imaging Pub Date : 2023-12-06 DOI: 10.1038/s44303-023-00003-1

Vito Ko, Marie C. Goess, Lukas Scheel-Platz, Tao Yuan, Andriy Chmyrov, Dominik Jüstel, Jürgen Ruland, Vasilis Ntziachristos, Selina J. Keppler, Miguel A. Pleitez

{"title":"Fast histological assessment of adipose tissue inflammation by label-free mid-infrared optoacoustic microscopy","authors":"Vito Ko, Marie C. Goess, Lukas Scheel-Platz, Tao Yuan, Andriy Chmyrov, Dominik Jüstel, Jürgen Ruland, Vasilis Ntziachristos, Selina J. Keppler, Miguel A. Pleitez","doi":"10.1038/s44303-023-00003-1","DOIUrl":"10.1038/s44303-023-00003-1","url":null,"abstract":"Conventional histology, as well as immunohistochemistry or immunofluorescence, enables the study of morphological and phenotypical changes during tissue inflammation with single-cell accuracy. However, although highly specific, such techniques require multiple time-consuming steps to apply exogenous labels, which might result in morphological deviations from native tissue structures. Unlike these techniques, mid-infrared (mid-IR) microspectroscopy is a label-free optical imaging method that retrieves endogenous biomolecular contrast without altering the native composition of the samples. Nevertheless, due to the strong optical absorption of water in biological tissues, conventional mid-IR microspectroscopy has been limited to dried thin (5–10 µm) tissue preparations and, thus, it also requires time-consuming steps—comparable to conventional imaging techniques. Here, as a step towards label-free analytical histology of unprocessed tissues, we applied mid-IR optoacoustic microscopy (MiROM) to retrieve intrinsic molecular contrast by vibrational excitation and, simultaneously, to overcome water-tissue opacity of conventional mid-IR imaging in thick (mm range) tissues. In this proof-of-concept study, we demonstrated application of MiROM for the fast, label-free, non-destructive assessment of the hallmarks of inflammation in excised white adipose tissue; i.e., formation of crown-like structures and changes in adipocyte morphology.","PeriodicalId":501709,"journal":{"name":"npj Imaging","volume":" ","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2023-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s44303-023-00003-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138866984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The chicken chorioallantoic membrane as a low-cost, high-throughput model for cancer imaging 鸡绒毛膜是一种低成本、高通量的癌症成像模型

npj Imaging Pub Date : 2023-11-29 DOI: 10.1038/s44303-023-00001-3

Lydia M. Smith, Hannah E. Greenwood, Will E. Tyrrell, Richard S. Edwards, Vittorio de Santis, Friedrich Baark, George Firth, Muhammet Tanc, Samantha Y. A. Terry, Anne Herrmann, Richard Southworth, Timothy H. Witney

{"title":"The chicken chorioallantoic membrane as a low-cost, high-throughput model for cancer imaging","authors":"Lydia M. Smith, Hannah E. Greenwood, Will E. Tyrrell, Richard S. Edwards, Vittorio de Santis, Friedrich Baark, George Firth, Muhammet Tanc, Samantha Y. A. Terry, Anne Herrmann, Richard Southworth, Timothy H. Witney","doi":"10.1038/s44303-023-00001-3","DOIUrl":"10.1038/s44303-023-00001-3","url":null,"abstract":"Mouse models are invaluable tools for radiotracer development and validation. They are, however, expensive, low throughput, and are constrained by animal welfare considerations. Here, we assessed the chicken chorioallantoic membrane (CAM) as an alternative to mice for preclinical cancer imaging studies. NCI-H460 FLuc cells grown in Matrigel on the CAM formed vascularized tumors of reproducible size without compromising embryo viability. By designing a simple method for vessel cannulation it was possible to perform dynamic PET imaging in ovo, producing high tumor-to-background signal for both 18F-2-fluoro-2-deoxy-D-glucose (18F-FDG) and (4S)-4-(3-18F-fluoropropyl)-L-glutamate (18F-FSPG). The pattern of 18F-FDG tumor uptake were similar in ovo and in vivo, although tumor-associated radioactivity was higher in the CAM-grown tumors over the 60 min imaging time course. Additionally, 18F-FSPG provided an early marker of both treatment response to external beam radiotherapy and target inhibition in ovo. Overall, the CAM provided a low-cost alternative to tumor xenograft mouse models which may broaden access to PET and SPECT imaging and have utility across multiple applications.","PeriodicalId":501709,"journal":{"name":"npj Imaging","volume":" ","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s44303-023-00001-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138866985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Projective oblique plane structured illumination microscopy 投射斜面结构照明显微镜

npj Imaging Pub Date : 2023-11-28 DOI: 10.1038/s44303-023-00002-2

Bo-Jui Chang, Douglas Shepherd, Reto Fiolka

引用次数: 0

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