The Plant Cell最新文献_第8页

A four-dimensional spatial transcriptome atlas of barley caryopsis development and germination. 大麦颖果发育和萌发的四维空间转录组图谱。

The Plant Cell Pub Date : 2026-04-21 DOI: 10.1093/plcell/koag120

Marta Peirats-Llobet,Zorana Staka,Xiujuan Yang,Yanqiao Zhu,Cunman He,Bhavna Hurgobin,Felipe Ayora,Maria Sofia L Yangzon,Monika W Murcha,Valencia Marisa,Ghazanfar Abbas Khan,Runxuan Zhang,Iain Milne,Huixia Shou,Matthew R Tucker,Mathew G Lewsey,James Whelan

{"title":"A four-dimensional spatial transcriptome atlas of barley caryopsis development and germination.","authors":"Marta Peirats-Llobet,Zorana Staka,Xiujuan Yang,Yanqiao Zhu,Cunman He,Bhavna Hurgobin,Felipe Ayora,Maria Sofia L Yangzon,Monika W Murcha,Valencia Marisa,Ghazanfar Abbas Khan,Runxuan Zhang,Iain Milne,Huixia Shou,Matthew R Tucker,Mathew G Lewsey,James Whelan","doi":"10.1093/plcell/koag120","DOIUrl":"https://doi.org/10.1093/plcell/koag120","url":null,"abstract":"A four-dimensional spatial gene expression atlas of Hordeum vulgare (barley) grain development and germination was generated using spatial transcriptomic analysis of serial sections to reconstruct transcript abundance in three physical dimensions and with temporal kinetics. We investigated the sub-tissue localisations of specific biological activities, using energy biology as an example, including genes encoding proteins involved in starch synthesis and degradation, sugar transport, mitochondrial and chloroplast activity. This atlas revealed different patterns in gene expression across tissues and developmental stages. Heterogeneity in gene expression was observed between clusters, within domains of the individual clusters, across two-dimensional (xy, 55 μm resolution) and three-dimensional (xyz, 8 μm resolution in z-plane) axes. Yet, other genes including typical housekeeping genes such as Actin, Tubulin and others, displayed homogeneous expression patterns. Expression of several genes matched previous gene-specific studies in different barley varieties verifying the robustness of the approach, and indicating that patterns of gene expression are conserved at least for some categories of genes between varieties. Trajectory analysis of aleurone tissue spanning from early development to the completion of germination, provided a comprehensive roadmap of tissue development in terms of processes and identified transcription factors with spatial specificity that play roles in seed development and germination. A public visualization browser is available to view two- and three-dimensional transcription abundance profiles at https://barley-4d.latrobe.edu.au/or https://barley-4d-gene-atlas.hutton.ac.uk/.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147731364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nodulin cleavage by the cysteine protease CYP35 promotes soybean root nodule senescence 半胱氨酸蛋白酶CYP35对根瘤蛋白的裂解促进了大豆根瘤的衰老

The Plant Cell Pub Date : 2026-04-21 DOI: 10.1093/plcell/koag123

Jiashan Wu, Weiyun Wang, Yuxin Guan, Ru Dong, Yan Duan, Aifang Xiao, Haoxing Li, Bingfu Guo, Xiaoli Guo, Hui Zhu, Yangrong Cao

{"title":"Nodulin cleavage by the cysteine protease CYP35 promotes soybean root nodule senescence","authors":"Jiashan Wu, Weiyun Wang, Yuxin Guan, Ru Dong, Yan Duan, Aifang Xiao, Haoxing Li, Bingfu Guo, Xiaoli Guo, Hui Zhu, Yangrong Cao","doi":"10.1093/plcell/koag123","DOIUrl":"https://doi.org/10.1093/plcell/koag123","url":null,"abstract":"In legumes, symbiotic root nodules undergo senescence in response to developmental or environmental cues. This process determines the maintenance and nitrogen-fixing capacity of the root nodules, but the molecular mechanisms underlying its initiation are poorly understood. The cysteine protease CYP35 is a positive regulator of nodule senescence in soybean (Glycine max), but its substrates remain unknown. Here, we demonstrate that CYP35 promotes nodule senescence by cleaving a subset of Nodule-Enriched Nodulin proteins (NENs). Sequence and phylogenetic analyses indicate that CYP35 is a cathepsin L-like cysteine protease, with Cys149 as a key catalytic residue. CYP35 physically interacts with a distinct subfamily of eight NENs, NEN1–8. Soybean quadruple and quintuple nen mutants obtained by multiplex gene editing develop nodules with accelerated senescence and reduced nitrogenase activity, whereas over-expression of NEN2 or NEN5 delays senescence and enhances nodule function. CYP35 proteolyzes NEN2, NEN5, NEN6, and NEN7 in vitro and cleaves NEN2 in vivo in a Cys149-dependent manner. Our findings establish a direct molecular link between cysteine protease–mediated Nodulin cleavage and the onset of nodule senescence in soybean, providing insights into the regulation of nodule lifespan and nitrogen fixation.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147733479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

OsNUOR enhances disease susceptibility by interfering with reactive oxygen species homeostasis and ferroptosis-like cell death. osnor通过干扰活性氧稳态和铁中毒样细胞死亡来增强疾病易感性。

The Plant Cell Pub Date : 2026-04-16 DOI: 10.1093/plcell/koag112

Debashis Sahoo,Ravindra K Chandan,Naveen Goel,Gopaljee Jha

{"title":"OsNUOR enhances disease susceptibility by interfering with reactive oxygen species homeostasis and ferroptosis-like cell death.","authors":"Debashis Sahoo,Ravindra K Chandan,Naveen Goel,Gopaljee Jha","doi":"10.1093/plcell/koag112","DOIUrl":"https://doi.org/10.1093/plcell/koag112","url":null,"abstract":"Necrotrophic fungal pathogens such as Rhizoctonia solani, the causal agent of rice (Oryza sativa) sheath blight disease, enhance reactive oxygen species (ROS) production to induce necrosis in infected tissues. Here, we present evidence that the host alternative NADH:ubiquinone oxidoreductase (OsNUOR) facilitates R. solani infection by promoting an oxidative-stress-enriched environment and inducing iron-dependent ferroptosis-like cell death. OsNUOR overexpression (OE) lines exhibit enhanced disease susceptibility, whereas knock-out (KO) lines developed through genome editing demonstrate increased resistance. Infected OE lines have enhanced accumulation of ROS, lipid peroxides, and ferric ions (Fe3+); a significant reduction in antioxidative enzyme (including glutathione peroxidase) activity; and depletion of glutathione levels. In KO lines, the redox status of infected tissues is maintained, and the antioxidative defense is activated. Our data suggest that upregulation of OsNUOR induces mitochondrial ROS accumulation and modulates redox signalling, leading to Fe3+ accumulation and lipid peroxidation that promote necrosis in rice. KO lines are compromised in these processes and therefore exhibit disease resistance. We demonstrate that treatment with ferroptosis inhibitors prevents necrotic lesions, whereas ferroptosis inducers enhance disease severity. Overall, our study reveals the importance of ferroptosis-like cell death in promoting necrosis during R. solani infection in rice.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147695075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Setting the stage: The sleek cell-cycle machinery of the liverwort Marchantia polymorpha. 舞台布景：多形地茅（Marchantia polymorpha）光滑的细胞周期机制。

The Plant Cell Pub Date : 2026-04-16 DOI: 10.1093/plcell/koag111

Julie Robinson

引用次数: 0

A cytological framework of female meiosis in Arabidopsis. 拟南芥雌性减数分裂的细胞学框架。

The Plant Cell Pub Date : 2026-04-16 DOI: 10.1093/plcell/koag113

Bingyan Hu,Maria Ada Prusicki,Katharina Stahlmann,Yingqi Wang,Arp Schnittger

引用次数: 0

CITF1 interacts with FIT and regulates copper-iron crosstalk in Arabidopsis. 拟南芥中CITF1与FIT相互作用并调控铜铁串扰。

The Plant Cell Pub Date : 2026-04-16 DOI: 10.1093/plcell/koag114

J C Chia,X Liu,S Dey,J Yan,Y Niu,O K Vatamaniuk

{"title":"CITF1 interacts with FIT and regulates copper-iron crosstalk in Arabidopsis.","authors":"J C Chia,X Liu,S Dey,J Yan,Y Niu,O K Vatamaniuk","doi":"10.1093/plcell/koag114","DOIUrl":"https://doi.org/10.1093/plcell/koag114","url":null,"abstract":"Iron (Fe) and copper (Cu) are essential yet potentially toxic metals with interconnected metabolic pathways; however, the mechanisms underlying Fe-Cu crosstalk remain poorly defined. Here, we show that CITF1 (COPPER DEFICIENCY INDUCED TRANSCRIPTION FACTOR 1), a Cu homeostasis regulator in Arabidopsis thaliana, physically interacts with FIT (FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR), the central Fe homeostasis regulator, forming a nutrient-responsive transcriptional module. Under Cu deficiency, the CITF1-FIT complex accumulates and promotes expression of the Cu uptake genes COPT2 (COPPER TRANSPORTER 2), FRO4 (FERRIC REDUCTION OXIDASE 4), and FRO5 (FERRIC REDUCTION OXIDASE 5). Proteasome-dependent degradation regulates CITF1 and FIT stability, with Cu deficiency delaying their turnover in a CITF1-dependent manner. Under Fe deficiency, CITF1 expression is downregulated, allowing FIT to interact with bHLH38/39/100/101 partners and activate Fe uptake genes, as CITF1 disrupts these interactions. Thus, CITF1 negatively regulates Fe acquisition. Consistent with this, citf1-1 and citf1-2 mutants show reduced sensitivity to Fe deficiency. Under Cu deficiency, the citf1-2 and fit-2 mutants have additive effects and under Fe deficiency, the double mutant shows partial suppression of the fit-2 slow growth phenotype, supporting the positive and negative roles of CITF1 in Cu and Fe homeostasis, respectively. Complete loss of CITF1 function in the homozygous citf1-1 fit-2 double mutant causes embryo lethality, revealing roles for CITF1 and FIT in embryo development. These findings establish CITF1 as a nutrient-responsive regulator of Cu/Fe crosstalk, functioning through interactions with FIT to prioritize Cu or Fe acquisition and balance micronutrient homeostasis.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147695073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Growth versus Decline: Root Aging and Plant Performance. 生长与衰退：根系老化与植物性能。

The Plant Cell Pub Date : 2026-04-16 DOI: 10.1093/plcell/koag115

Feng Qin,Wolfgang Busch

引用次数: 0

The LARGE3-OsHDT1 complex controls rice grain size and weight by modulating histone H4 acetylation of OsMKKK10. LARGE3-OsHDT1复合体通过调节OsMKKK10的组蛋白H4乙酰化来控制大米的粒度和重量。

The Plant Cell Pub Date : 2026-04-10 DOI: 10.1093/plcell/koag109

Guozheng Zhang,Junmin Wang,Xiaohui Jing,Sha Tang,Leiying Zheng,Linlin Zhang,Baolan Zhang,Ran Xu,Penggen Duan,Luojiang Huang,Ke Huang,Ying Gao,Limin Zhang,Jianqin Hao,Xianmin Diao,Yunhai Li

{"title":"The LARGE3-OsHDT1 complex controls rice grain size and weight by modulating histone H4 acetylation of OsMKKK10.","authors":"Guozheng Zhang,Junmin Wang,Xiaohui Jing,Sha Tang,Leiying Zheng,Linlin Zhang,Baolan Zhang,Ran Xu,Penggen Duan,Luojiang Huang,Ke Huang,Ying Gao,Limin Zhang,Jianqin Hao,Xianmin Diao,Yunhai Li","doi":"10.1093/plcell/koag109","DOIUrl":"https://doi.org/10.1093/plcell/koag109","url":null,"abstract":"Grain size is an important yield trait in cereal crops and understanding the mechanisms that control grain size is essential for precise breeding and improving cereal crop yields. Epigenetics plays a crucial role in plant growth and development, but how it is involved in grain size regulation remains largely unclear. Here we report that the histone-binding protein LARGE3 associates with the histone deacetylase OsHDT1 (HD-tuins 1) to control grain length by influencing histone H4 acetylation in rice (Oryza sativa). The large3 mutants form large and heavy grains because of increased cell proliferation. LARGE3 physically interacts with OsHDT1 to negatively regulate grain length. LARGE3 and OsHDT1 repress the expression of an important grain size gene OsMKKK10 by decreasing its histone H4 acetylation level and increasing its nucleosome density. Importantly, genome editing of LARGE3 homolog in foxtail millet (Setaria italica) causes large grains and increased grain yield. These findings reveal a molecular mechanism for LARGE3-OsHDT1 mediated epigenetic modulation of grain length by regulating histone H4 acetylation of OsMKKK10, suggesting that this module has potential applications in improving grain size and yield in key cereal crops.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147663743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterodimers of the MADS transcription factors GhAGL1 and GhAGL4 modulate cotton fiber initiation and elongation. MADS转录因子GhAGL1和GhAGL4的异源二聚体调节棉纤维的起始和伸长。

The Plant Cell Pub Date : 2026-04-10 DOI: 10.1093/plcell/koag107

Mengling Sun,Yawen Xiao,Xiaxia Liu,Yuqi Liu,Menghui Zheng,Tian Lei,Yirong Tao,Weiran Wang,Chunyuan You,Zixin Zhou,Xianlong Zhang,Jie Kong,Lili Tu

{"title":"Heterodimers of the MADS transcription factors GhAGL1 and GhAGL4 modulate cotton fiber initiation and elongation.","authors":"Mengling Sun,Yawen Xiao,Xiaxia Liu,Yuqi Liu,Menghui Zheng,Tian Lei,Yirong Tao,Weiran Wang,Chunyuan You,Zixin Zhou,Xianlong Zhang,Jie Kong,Lili Tu","doi":"10.1093/plcell/koag107","DOIUrl":"https://doi.org/10.1093/plcell/koag107","url":null,"abstract":"The initial number of fibers and fiber length affect fiber yield and quality in cotton (Gossypium hirsutum). The mechanism of fiber initiation centers on the transcription factor GhMYB25-like, and the involvement of other regulatory factors remains unclear. Here, we established a GhMYB25-like-based interaction network that regulates fiber initiation, and focused on the interacting protein AGAMOUS-like 4 (GhAGL4), which is a MIKC-MADS transcription factor, and other MIKC-MADS family members. Genome-wide identification of the cotton MIKC-MADS family members identified two highly co-expressed genes, GhAGL1 and GhAGL4, which are predominantly expressed in ovules at 0 days post-anthesis (DPA). CRISPR/Cas9-mediated loss of function of GhAGL1 or GhAGL4 led to a decrease in fiber initials and impeded fiber elongation, with the Ghagl1 Ghagl4 double mutant exhibiting a stronger phenotype. GhAGL1 and GhAGL4 interact in vitro and in vivo. DNA affinity purification and sequencing (DAP-seq) combined with RNA sequencing identified CCCH zinc finger 1 (GhCZF1) as a downstream gene of GhAGL1 and GhAGL4. Interestingly, GhMYB25-like enhances the ability of GhAGL1-GhAGL4 heterodimers to activate downstream genes. During the early fiber elongation stage, GhCZF1 activates the expression of the xyloglucan endotransglycosylase/hydrolase (XTH) genes GhXTH16 and GhXTH23 to regulate fiber elongation. Our findings reveal a molecular mechanism involved in fiber initiation and early elongation, providing a foundation for enhancing cotton fiber yield and quality.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"279 3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147663782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Orchestrating symbiosis: how bacterial auxin programs soybean nodulation. 协调共生：细菌生长素如何控制大豆结瘤。

The Plant Cell Pub Date : 2026-04-10 DOI: 10.1093/plcell/koag110

Ved Prakash

引用次数: 0