Journal of Rapid Methods and Automation in Microbiology最新文献_第5页

MOLECULAR DETECTION AND IDENTIFICATION OF ASPERGILLUS NIGER CONTAMINATION IN COSMETIC/PHARMACEUTICAL RAW MATERIALS AND FINISHED PRODUCTS 化妆品/药品原料和成品中黑曲霉污染的分子检测与鉴定

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-11-09 DOI: 10.1111/j.1745-4581.1999.tb00370.x

LUIS JIMENEZ, YOOPA BOSKO, STACEY SMALLS, RAYMOND IGNAR, DONALD ENGLISH

{"title":"MOLECULAR DETECTION AND IDENTIFICATION OF ASPERGILLUS NIGER CONTAMINATION IN COSMETIC/PHARMACEUTICAL RAW MATERIALS AND FINISHED PRODUCTS","authors":"LUIS JIMENEZ, YOOPA BOSKO, STACEY SMALLS, RAYMOND IGNAR, DONALD ENGLISH","doi":"10.1111/j.1745-4581.1999.tb00370.x","DOIUrl":"10.1111/j.1745-4581.1999.tb00370.x","url":null,"abstract":"Abstract A PCR-based assay using a one step sample preparation was developed and validated against standard methods for the rapid detection of Aspergillus niger contamination in cosmetic/pharmaceutical raw materials and finished products. Artificially contaminated samples were added to Sabouraud Dextrose Broth (SDB) with 10% Tween 20 and 3% Soy Lecithin. Samples were then agitated at 200 revolutions per minute for 24 h at 35C. Prior to the PCR assay, sample preparation consisted of adding 10 μL of the 24 h enrichment broth sample mixture to a lysis buffer containing Tris-EDTA and 0.4% SDS. DNA extraction was accomplished by boiling for 1 h to release the mycelial DNA. After DNA extraction, a 50 μL aliquot of the lysate was then added to a PCR tube containing the following ingredients: 4 Ready-To-Go PCR beads, 2 μL of two fungal primers, and 48 μL of sterile water. The DNA primers encoded for specific sequences of the Aspergillus spp. 18S rRNA gene. A 363 bp DNA fragment was detected in all of the artificially contaminated samples. Standard methods require 6–8 days to complete the isolation and identification of mold contamination. However, the PCR-based assay was completed within 27–30 h. Rapid detection of mold contamination in cosmetic/pharmaceutical raw materials and finished products can be used to optimize the quality evaluation of a sample to allow the release in 27–30 h.","PeriodicalId":50067,"journal":{"name":"Journal of Rapid Methods and Automation in Microbiology","volume":"7 1","pages":"39-46"},"PeriodicalIF":0.0,"publicationDate":"2011-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4581.1999.tb00370.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63411923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

RAPID DETECTION OF SALMONELLA TYPHIMURIUM USING AN IMMUNOELECTROCHEMICAL METHOD COUPLED WITH IMMUNOMAGNETIC SEPARATION 免疫电化学联用免疫磁分离快速检测鼠伤寒沙门菌

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-11-09 DOI: 10.1111/j.1745-4581.1999.tb00371.x

YIHUA CHE, ZHONGPING YANG, YANBIN LI, DAVID PAUL, MICHAEL SLAVIK

{"title":"RAPID DETECTION OF SALMONELLA TYPHIMURIUM USING AN IMMUNOELECTROCHEMICAL METHOD COUPLED WITH IMMUNOMAGNETIC SEPARATION","authors":"YIHUA CHE, ZHONGPING YANG, YANBIN LI, DAVID PAUL, MICHAEL SLAVIK","doi":"10.1111/j.1745-4581.1999.tb00371.x","DOIUrl":"10.1111/j.1745-4581.1999.tb00371.x","url":null,"abstract":"Abstract An immunoelectrochemical method coupled with magnetic separation was developed for rapid detection of Salmonella typhimurium. Samples containing Salmonella were mixed with anti-Salmonella coated magnetic beads (ASCMB) and alkaline phosphatase labeled anti-Salmonella (APLAS) simultaneously to form ASCMB-Sdhaoaella-APLAS conjugates by antibody-antigen reaction. The conjugates were separated by a magnetic field and were incubated with phenyl phosphate substrate to produce phenol. The number of Salmonella was determined by measuring the phenol concentration using an amperometric tyrosinase biosensor. This method could be completed within two hours with a detection limit of 5 times 103 cells/mL under optimized conditions (with ***1 mM MgCl2, 0.2 μg/mL APLAS and ***l mM phenyl phosphate in pH 7.0 TBS). A linear response on semi-log scale was found for Salmonella pure culture between 5 times 103 and 1 times 106 cells/mL.","PeriodicalId":50067,"journal":{"name":"Journal of Rapid Methods and Automation in Microbiology","volume":"7 1","pages":"47-59"},"PeriodicalIF":0.0,"publicationDate":"2011-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4581.1999.tb00371.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63411996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

GROWTH OF CAMPYLOBACTER JEJUNI IN VARIOUS MEDIA UNDER DIFFERENT ATMOSPHERIC CONDITIONS 不同大气条件下不同培养基中空肠弯曲杆菌的生长

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-11-09 DOI: 10.1111/j.1745-4581.1999.tb00400.x

BURTON W. BLAIS, L.M. PHILLIPPE

引用次数: 3

DEVELOPMENT OF A PCR ASSAY FOR DETECTION OF SPORE-FORMING BACTERIA 孢子形成细菌PCR检测方法的建立

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-11-09 DOI: 10.1111/j.1745-4581.1999.tb00397.x

EDNA F. ARCURI, MARTIN WIEDMANN, KATHRYN J. BOOR

{"title":"DEVELOPMENT OF A PCR ASSAY FOR DETECTION OF SPORE-FORMING BACTERIA","authors":"EDNA F. ARCURI, MARTIN WIEDMANN, KATHRYN J. BOOR","doi":"10.1111/j.1745-4581.1999.tb00397.x","DOIUrl":"10.1111/j.1745-4581.1999.tb00397.x","url":null,"abstract":"Abstract Degenerate PCR primers were designed based on the published nucleotide sequences of the sporulation sigma factor ***oE (spoIIGB) from Bacillus subtilis, Bacillus thuringiensis, and Clostridium acetobutylicum. The primer set was used in a Hot Start Touch Down-PCR to screen for the presence of the target gene in both spore-forming (eight Bacillus species, eight Clostridium species, Paenibacillus polymyxa, Thermoanaerobacterium thermosaccharolyticum, Moorella thermoacetica) and in nonspore-forming bacteria. Under optimized PCR conditions, all spore-forming bacteria tested yielded a PCR product of the expected size (∼360bp), although the nonspore-forming Listeria monocytogenes and Lactococcus lactis subsp. lactis also yielded PCR products of this approximate size. To improve the specificity and sensitivity of this assay, we Southern blotted gel electrophoresis-separated PCR products with a digoxigenin-labeled B. subtilis spoIIGB probe. This probe hybridized with the ∼ 360 bp PCR product from all spore-forming species but did not hybridize with PCR products of this approximate size from any nonspore-forming bacteria. The PCR-Southern blot assay was 100 to 1,000-fold more sensitive than PCR alone, yielding a lower detection limit of approximately 3 CFU spore-forming bacteria/PCR reaction. We conclude that, based on amplicon size and Southern hybridization, this strategy provides a viable approach for detecting spore-forming bacteria.","PeriodicalId":50067,"journal":{"name":"Journal of Rapid Methods and Automation in Microbiology","volume":"7 4","pages":"251-262"},"PeriodicalIF":0.0,"publicationDate":"2011-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4581.1999.tb00397.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63412812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thoughts on Someone Else's Retirement 对别人退休的想法

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-11-09 DOI: 10.1111/j.1745-4581.1999.tb00392.x

DANIEL Y.C. FUNG

引用次数: 0

DETECTION OF IMMUNOMAGNETIC BEAD CAPTURED ESCHERICHIA COLI O157:H7 BY LIGHT ADDRESSABLE POTENTIOMETRIC SENSOR† 光寻址电位传感器†检测免疫磁珠捕获的大肠杆菌o157: h7

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-11-09 DOI: 10.1111/j.1745-4581.1999.tb00376.x

SHU-I TU, JOSEPH UKNALIS, ANDREW GEHRING

引用次数: 18

COMPARISON OF MINIMAL MEDIA AND INOCULUM CONCENTRATION TO DECREASE THE LYSINE GROWTH ASSAY RESPONSE TIME OF AN ESCHERICHIA COLI LYSINE AUXOTROPH MUTANT 最小培养基和接种量对降低赖氨酸营养不良突变体赖氨酸生长试验响应时间的比较

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-11-09 DOI: 10.1111/j.1745-4581.1999.tb00399.x

X. LI, A.M. ERICKSON, S.C. RICKE

{"title":"COMPARISON OF MINIMAL MEDIA AND INOCULUM CONCENTRATION TO DECREASE THE LYSINE GROWTH ASSAY RESPONSE TIME OF AN ESCHERICHIA COLI LYSINE AUXOTROPH MUTANT","authors":"X. LI, A.M. ERICKSON, S.C. RICKE","doi":"10.1111/j.1745-4581.1999.tb00399.x","DOIUrl":"10.1111/j.1745-4581.1999.tb00399.x","url":null,"abstract":"Abstract Microbiological assays involving an Escherichia coli lysine auxotroph have been developed previously for the estimation of lysine bioavailability. To facilitate routine use of an auxotrophic E. coli lysine mutant for a microbial assay, our objectives in this study were to improve the assay with respect to shortening the assay time via increasing inoculum levels, and examine two different minimal media, a minimal salts medium (M9) and a more extensively supplemented minimal medium (DM). The maximum optical density (MOD) response of the E. coli in M9 medium was significantly (p < 0.05) lower than the MOD in the DM medium. However, the time to reach MOD in M9 medium was significantly (p < 0.05) shortened when compared to the time in the DM medium. The total assay time was also significantly shortened from a mean time 13.98 h to 8.19 h by using higher inoculum levels. The results from the study indicated that the E. coli lysine auxotroph bioassay time is shortened by cultivation of the assay bacteria in a more minimal medium and utilization of a more concentrated inocula.","PeriodicalId":50067,"journal":{"name":"Journal of Rapid Methods and Automation in Microbiology","volume":"7 4","pages":"279-290"},"PeriodicalIF":0.0,"publicationDate":"2011-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4581.1999.tb00399.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63413038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

PRELIMINARY PROGRAM KANSAS STATE UNIVERSITY XX GALA ANNIVERSARY INTERNATIONAL RAPID METHODS AND AUTOMATION IN MICROBIOLOGY WORKSHOP AGENDA* JULY 6–14, 2000 堪萨斯州立大学xx周年庆典国际微生物学快速方法和自动化研讨会议程* 2000年7月6日至14日

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-11-09 DOI: 10.1111/j.1745-4581.1999.tb00373.x

引用次数: 0

COMPARISON OF TWO RAPID METHODS FOR THE DETECTION OF LISTERIA MONOCYTOGENES WITH A STANDARD PROCEDURE IN NATURALLY CONTAMINATED ENVIRONMENTS OF RAW POULTRY PROCESSING 生禽加工自然污染环境中标准程序快速检测单核细胞增生李斯特菌的两种方法比较

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-11-09 DOI: 10.1111/j.1745-4581.1999.tb00384.x

E. CHASSEIGNAUX, Y. MICHEL, M.-T. TOQUIN, G. ERMEL, G. SALVAT, P. COLIN

引用次数: 5

EVALUATION OF THE TECRA™ VISUAL IMMUNOASSAY FOR DETECTING STAPHYLOCOCCUS AUREUS IN COSMETIC/PHARMACEUTICAL RAW MATERIALS AND FINISHED PRODUCTS 用于检测化妆品/制药原料和成品中金黄色葡萄球菌的tecra™视觉免疫分析法的评价

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-11-09 DOI: 10.1111/j.1745-4581.1999.tb00389.x

D. ENGLISH, C. SCALICI, J. HAMILTON, C. DESTRO, L. JIMENEZ

{"title":"EVALUATION OF THE TECRA™ VISUAL IMMUNOASSAY FOR DETECTING STAPHYLOCOCCUS AUREUS IN COSMETIC/PHARMACEUTICAL RAW MATERIALS AND FINISHED PRODUCTS","authors":"D. ENGLISH, C. SCALICI, J. HAMILTON, C. DESTRO, L. JIMENEZ","doi":"10.1111/j.1745-4581.1999.tb00389.x","DOIUrl":"10.1111/j.1745-4581.1999.tb00389.x","url":null,"abstract":"Abstract An assessment of the new TECRA™ Staphylococcus aureus Visual Immunoassay (VIA) was conducted to detect the presence of Staphylococcus aureus in cosmetic/pharmaceutical raw materials and finished products. Samples were inoculated with pure cultures of Staphylococcus aureus. In addition, a mixed bacterial inoculum of Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, and Pseudomonas aeruginosa was used to inoculate samples of raw materials and finished products. After a 24 h incubation period at 35C, each enrichment broth was analyzed for the presence of Staphylococcus aureus by the standard conventional method and the TECRA™ VIA. The following preenrichment broths were analyzed: the TECRA™ Staphylococcus Growth Medium, a modified version of the TECRA™ Staphylococcus Growth Medium with 4% Tween 20. Tryptic Soy Broth (TSB), and TSB with 4% Tween 20. Uninoculated enrichment broth samples were used as negative test controls. There was a 100% correlation between the visual immunoassay and the standard method in both pure and mixed culture studies for the detection of Staphylococcus aureus in preenrichment samples of the modified TECRA™ Staphylococcus Growth Medium and TSB. However, the TECRA™ VIA detected Staphylococcus aureus in 26 h while standard USP detection method required 4–5 days to be completed. These test results indicate that the TECRA™ Staphylococcus aureus VIA is equivalent to the conventional method for detecting the presence of Staphylococcus aureus contamination in cosmetic/pharmaceutical finished products and raw materials, but in a considerably shorter period of time. Furthermore, the TECRA™ Staphylococcus aureus VIA provides a user-friendly alternative to the standard conventional method for the detection of Staphylococcus aureus contamination.","PeriodicalId":50067,"journal":{"name":"Journal of Rapid Methods and Automation in Microbiology","volume":"7 3","pages":"193-203"},"PeriodicalIF":0.0,"publicationDate":"2011-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4581.1999.tb00389.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63412085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6