YIHUA CHE, ZHONGPING YANG, YANBIN LI, DAVID PAUL, MICHAEL SLAVIK
{"title":"免疫电化学联用免疫磁分离快速检测鼠伤寒沙门菌","authors":"YIHUA CHE, ZHONGPING YANG, YANBIN LI, DAVID PAUL, MICHAEL SLAVIK","doi":"10.1111/j.1745-4581.1999.tb00371.x","DOIUrl":null,"url":null,"abstract":"<p><b>Abstract </b> <i>An immunoelectrochemical method coupled with magnetic separation was developed for rapid detection of</i> Salmonella typhimurium. <i>Samples containing</i> Salmonella <i>were mixed with anti</i>-Salmonella <i>coated magnetic beads (ASCMB) and alkaline phosphatase labeled anti</i>-Salmonella <i>(APLAS) simultaneously to form ASCMB</i>-Sdhaoaella-<i>APLAS conjugates by antibody-antigen reaction. The conjugates were separated by a magnetic field and were incubated with phenyl phosphate substrate to produce phenol. The number of</i> Salmonella <i>was determined by measuring the phenol concentration using an amperometric tyrosinase biosensor. This method could be completed within two hours with a detection limit of 5 times 10<sup>3</sup> cells/mL under optimized conditions (with ***1 mM MgCl<sub>2</sub>, 0.2 μg/mL APLAS and ***l mM phenyl phosphate in pH 7.0 TBS). A linear response on semi-log scale was found for</i> Salmonella <i>pure culture between 5 times 10<sup>3</sup> and 1 times 10<sup>6</sup> cells/mL.</i></p>","PeriodicalId":50067,"journal":{"name":"Journal of Rapid Methods and Automation in Microbiology","volume":"7 1","pages":"47-59"},"PeriodicalIF":0.0000,"publicationDate":"2011-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4581.1999.tb00371.x","citationCount":"24","resultStr":"{\"title\":\"RAPID DETECTION OF SALMONELLA TYPHIMURIUM USING AN IMMUNOELECTROCHEMICAL METHOD COUPLED WITH IMMUNOMAGNETIC SEPARATION\",\"authors\":\"YIHUA CHE, ZHONGPING YANG, YANBIN LI, DAVID PAUL, MICHAEL SLAVIK\",\"doi\":\"10.1111/j.1745-4581.1999.tb00371.x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><b>Abstract </b> <i>An immunoelectrochemical method coupled with magnetic separation was developed for rapid detection of</i> Salmonella typhimurium. <i>Samples containing</i> Salmonella <i>were mixed with anti</i>-Salmonella <i>coated magnetic beads (ASCMB) and alkaline phosphatase labeled anti</i>-Salmonella <i>(APLAS) simultaneously to form ASCMB</i>-Sdhaoaella-<i>APLAS conjugates by antibody-antigen reaction. The conjugates were separated by a magnetic field and were incubated with phenyl phosphate substrate to produce phenol. The number of</i> Salmonella <i>was determined by measuring the phenol concentration using an amperometric tyrosinase biosensor. This method could be completed within two hours with a detection limit of 5 times 10<sup>3</sup> cells/mL under optimized conditions (with ***1 mM MgCl<sub>2</sub>, 0.2 μg/mL APLAS and ***l mM phenyl phosphate in pH 7.0 TBS). A linear response on semi-log scale was found for</i> Salmonella <i>pure culture between 5 times 10<sup>3</sup> and 1 times 10<sup>6</sup> cells/mL.</i></p>\",\"PeriodicalId\":50067,\"journal\":{\"name\":\"Journal of Rapid Methods and Automation in Microbiology\",\"volume\":\"7 1\",\"pages\":\"47-59\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2011-11-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1111/j.1745-4581.1999.tb00371.x\",\"citationCount\":\"24\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Rapid Methods and Automation in Microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/j.1745-4581.1999.tb00371.x\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Rapid Methods and Automation in Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/j.1745-4581.1999.tb00371.x","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
RAPID DETECTION OF SALMONELLA TYPHIMURIUM USING AN IMMUNOELECTROCHEMICAL METHOD COUPLED WITH IMMUNOMAGNETIC SEPARATION
Abstract An immunoelectrochemical method coupled with magnetic separation was developed for rapid detection of Salmonella typhimurium. Samples containing Salmonella were mixed with anti-Salmonella coated magnetic beads (ASCMB) and alkaline phosphatase labeled anti-Salmonella (APLAS) simultaneously to form ASCMB-Sdhaoaella-APLAS conjugates by antibody-antigen reaction. The conjugates were separated by a magnetic field and were incubated with phenyl phosphate substrate to produce phenol. The number of Salmonella was determined by measuring the phenol concentration using an amperometric tyrosinase biosensor. This method could be completed within two hours with a detection limit of 5 times 103 cells/mL under optimized conditions (with ***1 mM MgCl2, 0.2 μg/mL APLAS and ***l mM phenyl phosphate in pH 7.0 TBS). A linear response on semi-log scale was found for Salmonella pure culture between 5 times 103 and 1 times 106 cells/mL.
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