Journal of Rapid Methods and Automation in Microbiology最新文献_第6页

EFFECTS OF MEMBRANE PROTONOPHORES ON THE ATP CONTENT OF IMMUNOMAGNETIC BEADS CAPTURED ESCHERICHIA COLI O157:H7 膜原载体对捕获大肠杆菌o157: h7免疫磁珠atp含量的影响

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-11-09 DOI: 10.1111/j.1745-4581.1999.tb00390.x

SHU-I TU, DEIDRE PATTERSON, LINDA S. L. YU, PETER IRWIN

{"title":"EFFECTS OF MEMBRANE PROTONOPHORES ON THE ATP CONTENT OF IMMUNOMAGNETIC BEADS CAPTURED ESCHERICHIA COLI O157:H7","authors":"SHU-I TU, DEIDRE PATTERSON, LINDA S. L. YU, PETER IRWIN","doi":"10.1111/j.1745-4581.1999.tb00390.x","DOIUrl":"10.1111/j.1745-4581.1999.tb00390.x","url":null,"abstract":"An approach to rapidly detect the presence of viable Escherichia coli O157:H7 is described. Specific immunomagnetic beads were applied to capture the bacteria in different solutions. The captured bacteria were then lysed by commercially available reagents to release cellular ATP which was detected by firefly luciferin-luciferase induced chemiluminescence. The bioenergetic status of the bacteria was adjusted by the addition of glucose, a carbon nutrient source, and carbonyl cyanide meta-chlorophenyl hydrazone (CCCP), a membrane protonophore. The addition of glucose restored oxygen consumption and medium acidification activities and increased the ATP content of harvested bacteria after storage. On the other hand, CCCP enhanced the oxygen consumption and medium acidification but significantly decreased the ATP content. None of the glucose and CCCP effects could be detected with heat-killed and γ-ray irradiated E. coli O157:H7. Thus, immunomagnetic capture of the E. coli followed by testing the bioenergetic responses of captured bacteria would qualitatively determine the presence of viable E. coli O157:H7. When applied, the developed procedure could easily detect the presence of less than one CFU of the E. coli per gram of beef hamburg after a 6-h enrichment at 37C.","PeriodicalId":50067,"journal":{"name":"Journal of Rapid Methods and Automation in Microbiology","volume":"7 3","pages":"205-221"},"PeriodicalIF":0.0,"publicationDate":"2011-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4581.1999.tb00390.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63412111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

PERFORMANCE OF PETRIFILM™ KIT-HEC FOR ENUMERATION OF ESCHERICHIA COLI O157:H7 IN ARTIFICIALLY CONTAMINATED GROUND BEEF petrifilm™试剂盒- hec在人工污染碎牛肉中大肠杆菌o157: h7计数的性能

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-11-09 DOI: 10.1111/j.1745-4581.1999.tb00387.x

SUSANA M. I. SAAD, BERNADETTE D.G.M. FRANCO

引用次数: 1

RAPID MICROBIAL GROWTH ON POLYCHLORINATED BIPHENYL AND ITS FATE IN CELLS 微生物对多氯联苯的快速生长及其在细胞中的命运

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-11-09 DOI: 10.1111/j.1745-4581.1999.tb00396.x

M.K HAMDY, Y.C. LIN, R. TOLEDO

{"title":"RAPID MICROBIAL GROWTH ON POLYCHLORINATED BIPHENYL AND ITS FATE IN CELLS","authors":"M.K HAMDY, Y.C. LIN, R. TOLEDO","doi":"10.1111/j.1745-4581.1999.tb00396.x","DOIUrl":"10.1111/j.1745-4581.1999.tb00396.x","url":null,"abstract":"Abstract Nine cultures were examined for growth at 37C in media with different levels of PCB and selected one culture to treat an industrial liquid waste containing high levels of PCB 1248. All the nine cultures grew well in the presence of 100 to 500 μg PCB 1254 mL−1 glucose basal salt broth (GBSB) as well as in tryptic soy broth (TSP) with no significant changes in generation time. The cultures grew well in basal salt agar containing PCB as sole source of carbon. Pseudomonas aeruginosa and Serratia liquefaciens strains survived for 75 to 120 min in the presence of 300 to 2000 μg PCB 1254 mL−1 sodium-phosphate buffer (SPB) pH 7.0. The P. aeruginosa cells removed both PCB 1254 and 14C-PCB from SPB and GBSB within 48 h and the percent uptakes were 26 and 39.7%, respectively. The results of the fate and distribution of PCB 1254, including 14C-PCB, in cells of P. aeruginosa, S. liquefaciens, and two Bacillus strains grown in PCB-media showed that more than 50% of the 14C-PCB were in the alcohol and in alcohol-ether soluble fractions (mostly lipids). This was also confirmed after rupturing the P. aeruginosa cells using French pressure and/or lysozyme and fractionation. Both the intrinsic flora and P. aeruginosa strain were used in a bioreactor system for the treatments of 27 and 33 gallons industrial liquid waste containing 150 and/or 800 mg PCB 12481 1−1, respectively, and complete oxidation was achieved, within approximately 150 days and the biological half-life of the PCB was 30 and 15 days, respectively.","PeriodicalId":50067,"journal":{"name":"Journal of Rapid Methods and Automation in Microbiology","volume":"7 4","pages":"233-249"},"PeriodicalIF":0.0,"publicationDate":"2011-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4581.1999.tb00396.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63412744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

GROWTH RESPONSE AND RECOVERY IN SELECTIVE MEDIA OF A LYSINE AUXOTROPH ESCHERICHIA COLI FOR A RAPID MICROBIOLOGICAL ASSAY 赖氨酸营养不良大肠杆菌在选择性培养基中的生长反应和恢复，用于快速微生物测定

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-11-09 DOI: 10.1111/j.1745-4581.1999.tb00398.x

I.B. ZABALA DÍAZ, A.M. ERICKSON, S.C. RICKE

{"title":"GROWTH RESPONSE AND RECOVERY IN SELECTIVE MEDIA OF A LYSINE AUXOTROPH ESCHERICHIA COLI FOR A RAPID MICROBIOLOGICAL ASSAY","authors":"I.B. ZABALA DÍAZ, A.M. ERICKSON, S.C. RICKE","doi":"10.1111/j.1745-4581.1999.tb00398.x","DOIUrl":"10.1111/j.1745-4581.1999.tb00398.x","url":null,"abstract":"Abstract Human foods and animal feeds vary in their amino acid availability based upon the nature of the protein source and subsequent processing treatments to which the source may have been subjected during manufacture. In this study, growth and recovery of an Escherichia coli lysine auxotroph assay organism was tested in the presence of an antibiotic and antifungal supplemented medium previously developed. Overall growth rate comparisons in amended liquid minimal media showed that addition of antistatic agents did not alter the growth rate of the indicator strain and that it is independent of lysine concentration. Six different animal feeds were studied to determine the potential background contribution of indigenous feed Escherichia coli and whether the selective medium would suppress these organisms. Recovery of the indicator strain used for the rapid bacterial lysine assay was above 94% in all feed suspensions. In addition to this, indigenous microflora of the animal feeds was unable to grow in the presence of the antistatic agents selected. Microbial growth measured as agar plate colonies from short (1 week) and long term storage (6 months) feeds were completely suppressed on the antibiotic supplemented plates after 24 h of incubation. This result confirms that the amendments will suppress the growth of indigenous feed E. coli populations during the time frame typically used to conduct the rapid bacterial lysine assay with the E. coli lysine auxotroph without altering the growth rate response of the auxotroph.","PeriodicalId":50067,"journal":{"name":"Journal of Rapid Methods and Automation in Microbiology","volume":"7 4","pages":"263-278"},"PeriodicalIF":0.0,"publicationDate":"2011-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4581.1999.tb00398.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63412949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

20th Gala Anniversary Celebration of the Workshop July 2000 工作坊二十周年庆典2000年7月

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-11-09 DOI: 10.1111/j.1745-4581.1999.tb00372.x

DANIEL Y.C. FUNG

引用次数: 0

HYDROGEN-DEUTERIUM EXCHANGE AND ULTRAVIOLET RESONANCE RAMAN SPECTROSCOPY OF BACTERIA IN A COMPLEX FOOD MATRIX† 复杂食物基质中细菌的氢-氘交换和紫外共振拉曼光谱研究

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-11-09 DOI: 10.1111/j.1745-4581.1999.tb00369.x

GREGORY P. HARHAY, GREGORY R. SIRAGUSA

引用次数: 5

NEW METHOD FOR EVALUATING BACTERIAL REDUCTION OF TRIMETHYLAMINE N-OXIDE AND ITS APPLICATION TO BACTERIAL POPULATIONS IN FISH MUSCLE 评价细菌还原三甲胺n -氧化物的新方法及其在鱼类肌肉细菌种群中的应用

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-11-09 DOI: 10.1111/j.1745-4581.1999.tb00381.x

MICHEL VALLÉ, PHILIPPE EB, ROGER TAILLIEZ, PIERRE MALLE

{"title":"NEW METHOD FOR EVALUATING BACTERIAL REDUCTION OF TRIMETHYLAMINE N-OXIDE AND ITS APPLICATION TO BACTERIAL POPULATIONS IN FISH MUSCLE","authors":"MICHEL VALLÉ, PHILIPPE EB, ROGER TAILLIEZ, PIERRE MALLE","doi":"10.1111/j.1745-4581.1999.tb00381.x","DOIUrl":"10.1111/j.1745-4581.1999.tb00381.x","url":null,"abstract":"Abstract Bacterial reduction of trimethylamine N-oxide (TMAO) cannot be reliably determined try qualitative methods, which are unusable because of problems relating to incubation time, the indicator and the equilibrium of the redox potential. This makes it difficult to reproduce results. These problems have been observed with various semi-agar media used in the evaluation of TMAO reduction. We propose a new and rapid method of quantitative evaluation by means of assay of trimethylamine (TMA) in a new TMA-free culture medium. This methodology has been used to evaluate the TMAO-reducing capacity of different endogenous and exogenous bacterial strains found in fish flesh (Aeromonas hydrophila, Alteromonas communis, Escherichia coli, Flavobacterium branchiophilum, Micrococcus sedentarius, Proteus mirabilis, Pseudomonas fluorescens, Pseudomonas nautica, Serratia marcescens, Shewanella putrefaciens, Vibrio parahaemolyticus). Unlike the qualitative methods, our method showed that all tested strains were able to reduce TMAO. Fish spoilage bacteria can form TMA under anaerobic conditions, as shown by tests using bacterial suspensions from fish (Helicolenus dactylopterus, Merlangus merlangus, Clupea harengus). Such tests can be used to assess fish spoilage.","PeriodicalId":50067,"journal":{"name":"Journal of Rapid Methods and Automation in Microbiology","volume":"7 2","pages":"119-133"},"PeriodicalIF":0.0,"publicationDate":"2011-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4581.1999.tb00381.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63411607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Safe Eating. 1998. David W. K. Acheson and Robin K. Levinson 《安全饮食》，1998。大卫·艾奇逊和罗宾·列文森

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-11-09 DOI: 10.1111/j.1745-4581.1999.tb00365.x

WILLIAM R. JACOB

引用次数: 0

A RAPID SCREENING METHOD FOR SALMONELLA TYPHIMURIUM DT 104 IN POULTRY BARN ENVIRONMENTAL SAMPLES 禽舍环境样品中鼠伤寒沙门菌d104快速筛选方法研究

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-11-09 DOI: 10.1111/j.1745-4581.1999.tb00386.x

STEPHEN J. SHAW, BURTON W. BLAIS, DEV C. NUNDY, RICHARD L. POCOCK

{"title":"A RAPID SCREENING METHOD FOR SALMONELLA TYPHIMURIUM DT 104 IN POULTRY BARN ENVIRONMENTAL SAMPLES","authors":"STEPHEN J. SHAW, BURTON W. BLAIS, DEV C. NUNDY, RICHARD L. POCOCK","doi":"10.1111/j.1745-4581.1999.tb00386.x","DOIUrl":"10.1111/j.1745-4581.1999.tb00386.x","url":null,"abstract":"Abstract A rapid screening method was developed to presumptively identify S. typhimurium DT 104 in naturally contaminated poultry barn environmental samples. This technique involves a semiselective preenrichment (chloramphenicol in buffered peptone water) procedure followed by screening with a Salmonella-specific chemiluminescence-based detection system (IsoSCREEN™, Stratecon Diagnostics International, USA), then plating on Modified Semi-solid Rappaport-Vassilliadis (MSRV) agar plates incorporating antibiotic disks (ampicillin, chloramphenicol and tetracycline). Environmental samples (183 total) from a poultry barn were analyzed for the suspected presence of Salmonella typhimurium DT 104. Of these, 141 samples were identified as containing Salmonella species, 9 of which were identified as Salmonella typhimurium DT 104 by both the standard culture methods and the combined IsoSCREEN™-MSRV antibiotic susceptibility test (IsoSCREEN™-MAST). In comparison with the standard culture and antibiotic susceptibility testing techniques, which require 7–9 days to complete, the IsoSCREEN™-MAST required only 3–5 days to presumptively identify Salmonella typhimurium DT 104 in the samples.","PeriodicalId":50067,"journal":{"name":"Journal of Rapid Methods and Automation in Microbiology","volume":"7 3","pages":"163-171"},"PeriodicalIF":0.0,"publicationDate":"2011-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4581.1999.tb00386.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63412204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KANSAS STATE UNIVERSITY INTERNATIONAL RAPID METHODS AND AUTOMATION IN MICROBIOLOGY WORKSHOP XIX TENTATIVE AGENDA -JULY 9–16,1999 1999年7月9日至16日，美国堪萨斯州立大学国际微生物学快速方法与自动化研讨会第十九届暂定议程

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-11-09 DOI: 10.1111/j.1745-4581.1999.tb00367.x

引用次数: 0