{"title":"petrifilm™试剂盒- hec在人工污染碎牛肉中大肠杆菌o157: h7计数的性能","authors":"SUSANA M. I. SAAD, BERNADETTE D.G.M. FRANCO","doi":"10.1111/j.1745-4581.1999.tb00387.x","DOIUrl":null,"url":null,"abstract":"<p><b>Abstract </b> <i>Petrifilm™ Kit-HEC (3M Company, St. Paul, MN) was used to monitor the growth of</i> Escherichia coli <i>O157:H7 in ground beef co-inoculated with microorganisms of the background flora, including nonpathogenic</i> E.coli, Pseudomonas putida <i>or</i> Leuconostoc <i>sp. The growth was monitored in beef samples stored at room temperature and under refrigeration. A cultural procedure using sorbitol MacConkey agar was used for comparison. The correlation between counts of</i> E.coli <i>O157:H7 using these procedures was high: 99.1% for samples kept at room temperature and 94.9% for those kept under refrigeration.</i></p>","PeriodicalId":50067,"journal":{"name":"Journal of Rapid Methods and Automation in Microbiology","volume":"7 3","pages":"173-181"},"PeriodicalIF":0.0000,"publicationDate":"2011-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4581.1999.tb00387.x","citationCount":"1","resultStr":"{\"title\":\"PERFORMANCE OF PETRIFILM™ KIT-HEC FOR ENUMERATION OF ESCHERICHIA COLI O157:H7 IN ARTIFICIALLY CONTAMINATED GROUND BEEF\",\"authors\":\"SUSANA M. I. SAAD, BERNADETTE D.G.M. FRANCO\",\"doi\":\"10.1111/j.1745-4581.1999.tb00387.x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><b>Abstract </b> <i>Petrifilm™ Kit-HEC (3M Company, St. Paul, MN) was used to monitor the growth of</i> Escherichia coli <i>O157:H7 in ground beef co-inoculated with microorganisms of the background flora, including nonpathogenic</i> E.coli, Pseudomonas putida <i>or</i> Leuconostoc <i>sp. The growth was monitored in beef samples stored at room temperature and under refrigeration. A cultural procedure using sorbitol MacConkey agar was used for comparison. The correlation between counts of</i> E.coli <i>O157:H7 using these procedures was high: 99.1% for samples kept at room temperature and 94.9% for those kept under refrigeration.</i></p>\",\"PeriodicalId\":50067,\"journal\":{\"name\":\"Journal of Rapid Methods and Automation in Microbiology\",\"volume\":\"7 3\",\"pages\":\"173-181\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2011-11-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1111/j.1745-4581.1999.tb00387.x\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Rapid Methods and Automation in Microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/j.1745-4581.1999.tb00387.x\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Rapid Methods and Automation in Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/j.1745-4581.1999.tb00387.x","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
摘要
Petrifilm™Kit-HEC (3M Company, St. Paul, MN)用于监测与背景菌群微生物(包括非致病性大肠杆菌、恶臭假单胞菌或Leuconostoc sp)共接种的碎牛肉中大肠杆菌O157:H7的生长情况。在室温和冷藏保存的牛肉样品中监测其生长情况。采用山梨醇麦康基琼脂培养方法进行比较。使用这些方法的大肠杆菌O157:H7计数之间的相关性很高:室温保存的样品为99.1%，冷藏保存的样品为94.9%。
PERFORMANCE OF PETRIFILM™ KIT-HEC FOR ENUMERATION OF ESCHERICHIA COLI O157:H7 IN ARTIFICIALLY CONTAMINATED GROUND BEEF
Abstract Petrifilm™ Kit-HEC (3M Company, St. Paul, MN) was used to monitor the growth of Escherichia coli O157:H7 in ground beef co-inoculated with microorganisms of the background flora, including nonpathogenic E.coli, Pseudomonas putida or Leuconostoc sp. The growth was monitored in beef samples stored at room temperature and under refrigeration. A cultural procedure using sorbitol MacConkey agar was used for comparison. The correlation between counts of E.coli O157:H7 using these procedures was high: 99.1% for samples kept at room temperature and 94.9% for those kept under refrigeration.
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