Journal of Rapid Methods and Automation in Microbiology最新文献_第7页

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-11-09 DOI: 10.1111/j.1745-4581.1999.tb00374.x

引用次数: 0

NOVEL METHOD FOR STUDY OF THE EXTRACELLULAR CELL-BOUND PROTEINASE FROM LACTIC ACID BACTERIA† 研究乳酸菌胞外细胞结合蛋白酶的新方法

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-11-09 DOI: 10.1111/j.1745-4581.1999.tb00380.x

DANIEL Y.C. FUNG, DONGHYUN KANG, THANGPONG PAILIN, KAREN A. SCHMIDT

引用次数: 0

A COMPARISON OF THE HEALTH PROTECTION BRANCH AND THE ENZYME LINKED FLUORESCENT ASSAY METHODS FOR THE ISOLATION AND IDENTIFICATION OF LISTERIA SPP. AND LISTERIA MONOCYTOGENES FROM FOODS 食品中李斯特菌和单核增生李斯特菌的保健分支法和酶联荧光法分离鉴定比较

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-11-09 DOI: 10.1111/j.1745-4581.1999.tb00388.x

E.F. DALEY, D. BOOTSVELD, D.W. WARBURTON, J.M. FARBER

{"title":"A COMPARISON OF THE HEALTH PROTECTION BRANCH AND THE ENZYME LINKED FLUORESCENT ASSAY METHODS FOR THE ISOLATION AND IDENTIFICATION OF LISTERIA SPP. AND LISTERIA MONOCYTOGENES FROM FOODS","authors":"E.F. DALEY, D. BOOTSVELD, D.W. WARBURTON, J.M. FARBER","doi":"10.1111/j.1745-4581.1999.tb00388.x","DOIUrl":"10.1111/j.1745-4581.1999.tb00388.x","url":null,"abstract":"Abstract The conventional Health Protection Branch method (MFHPB-30) for the detection of Listeria monocytogenes is labor intensive and may require seven days to complete, depending on the initial levels of the organism in the food sample. Thus, there is a definite need for a rapid isolation and detection method. There are two commercially available versions of the enzyme linked fluorescent assay (ELFA) method for identifying Listeria from foods. One identifies Listeria to the genus level and the other to the species (L. monocytogenes) level, with both giving results within 50 h. In the present study, the two ELFA methods were compared to the HPB method to determine their efficiency.For the genus-specific test, 326 uninoculated foods were compared by both the HPB and ELFA methods. When comparing the HPB method to the genus-specific ELFA method, the latter had a false-negative rate of 4.6%. There were no false positives. For the species-specific ELFA assay, of the 186 foods compared by the two methods, a false-negative rate of 8.1% and a false-positive rate of 3.8% was observed in the ELFA method as compared to the HPB method.","PeriodicalId":50067,"journal":{"name":"Journal of Rapid Methods and Automation in Microbiology","volume":"7 3","pages":"183-192"},"PeriodicalIF":0.0,"publicationDate":"2011-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4581.1999.tb00388.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63412366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

INTEGRATION OF HYDROXYAPATITE CONCENTRATION OF BACTERIA AND SEMINESTED PCR TO ENHANCE DETECTION OF SALMONELLA TYPHIMURIUM FROM GROUND BEEF AND BOVINE CARCASS SPONGE SAMPLES 结合细菌羟基磷灰石浓度和半植式PCR提高对牛肉碎和牛胴体海绵样品中鼠伤寒沙门菌的检测

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-11-09 DOI: 10.1111/j.1745-4581.1999.tb00411.x

ELAINE D. BERRY, GREGORY R. SIRAGUSA

{"title":"INTEGRATION OF HYDROXYAPATITE CONCENTRATION OF BACTERIA AND SEMINESTED PCR TO ENHANCE DETECTION OF SALMONELLA TYPHIMURIUM FROM GROUND BEEF AND BOVINE CARCASS SPONGE SAMPLES","authors":"ELAINE D. BERRY, GREGORY R. SIRAGUSA","doi":"10.1111/j.1745-4581.1999.tb00411.x","DOIUrl":"10.1111/j.1745-4581.1999.tb00411.x","url":null,"abstract":"Abstract Hydroxyapatite (HA) concentration of bacteria from ground beef and bovine carcass sponge samples was examined as a method to enhance the PCR detection of Salmonella typhimurium in these samples. Ground beef and carcass sponge samples were inoculated with progressively lower levels of S. typhimurium. Concentrated (10% HA) and unconcentrated samples were prepared for PCR after 0, 2, 3, or 4 h of nonselective enrichment. Without HA concentration and enrichment, Salmonella in ground beef was not detected by seminested PCR, even when present at levels of 105 cells/mL in the 1:10 ground beef homogenates. However, when bacteria in these samples were extracted with HA, limits of detection in nonenriched samples were 102-103 CFU/mL and in enriched samples were 101 CFU/mL (after 2 and 3 h enrichment) and 100 CFU/mL (after 4 h enrichment). Without concentration or enrichment, the limit of detection of Salmonella in carcass sponge samples was 103 cells/mL. HA concentration of these samples lowered this limit to 100 – 101 CFU/mL. Nine of 14 different Salmonella serotypes adhered to HA at proportions of 98.0% or more.","PeriodicalId":50067,"journal":{"name":"Journal of Rapid Methods and Automation in Microbiology","volume":"7 1","pages":"7-23"},"PeriodicalIF":0.0,"publicationDate":"2011-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4581.1999.tb00411.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63412642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

EDITOR'S CORNER 编辑器的角落

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-11-09 DOI: 10.1111/j.1745-4581.1999.tb00364.x

DANIEL Y.C. FUNG

引用次数: 0

Gala 20th Anniversary International Workshop/Symposium Rapid Methods and Automation in Microbiology July 6–14, 2000 2000年7月6日至14日举行的微生物学快速方法与自动化国际研讨会

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-11-09 DOI: 10.1111/j.1745-4581.1999.tb00393.x

引用次数: 0

IMAGE ANALYSIS OF COLONY SIZE FOR INVESTIGATING SUBLETHAL INJURY IN LISTERIA MONOCYTOGENES 研究单核增生李斯特菌亚致死损伤的菌落大小图像分析

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-11-09 DOI: 10.1111/j.1745-4581.1999.tb00395.x

GARY A. DYKES

{"title":"IMAGE ANALYSIS OF COLONY SIZE FOR INVESTIGATING SUBLETHAL INJURY IN LISTERIA MONOCYTOGENES","authors":"GARY A. DYKES","doi":"10.1111/j.1745-4581.1999.tb00395.x","DOIUrl":"10.1111/j.1745-4581.1999.tb00395.x","url":null,"abstract":"Abstract The traditional method for measuring sublethal injury in bacteria involves differential plating of injured cells on selective and nonselective media. Image analysis of the size of colonies from injured Listeria monocytogenes cells plated on nonselective media only was investigated as an alternative. With colonies from healthy cells, colony area was normally distributed, but heat- and starvation-stressed cells produced colonies with areas that showed a significant (p ≤ 0.05) skewness to the right. Although no relationship between sublethal injury and skewness was apparent, mean colony area was linearly (r2 = 0.90) related to sublethal injury. Mean colony area can therefore be used as a measure of sublethal injury in Listeria monocytogenes, eliminating the need for differential plating in certain experiments examining this phenomenon. Since significantly different (P ≤ 0.05) skewness was apparent between colonies derived from heat-stressed cells as opposed to those derived from starvation-stressed cells, this method also provides further information on variations in injury between individual cells within stressed populations. Due to variations in colony size between strains, the method is limited in many cases to application in single-culture studies.","PeriodicalId":50067,"journal":{"name":"Journal of Rapid Methods and Automation in Microbiology","volume":"7 4","pages":"223-231"},"PeriodicalIF":0.0,"publicationDate":"2011-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4581.1999.tb00395.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63412678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

MULTI-PIPETTE SYSTEM FOR RECOVERY OF LISTERIA SPP. AND SALMONELLA SPP. SIMULTANEOUSLY† 同时回收李斯特菌和沙门氏菌的多移液管系统

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-11-09 DOI: 10.1111/j.1745-4581.1999.tb00378.x

DONG-HYUN KANG, DANIEL Y.C. FUNG, GUANCHENG JIANG

{"title":"MULTI-PIPETTE SYSTEM FOR RECOVERY OF LISTERIA SPP. AND SALMONELLA SPP. SIMULTANEOUSLY†","authors":"DONG-HYUN KANG, DANIEL Y.C. FUNG, GUANCHENG JIANG","doi":"10.1111/j.1745-4581.1999.tb00378.x","DOIUrl":"10.1111/j.1745-4581.1999.tb00378.x","url":null,"abstract":"Abstract A new rapid method to simultaneously isolate Listeria spp. and Salmonella spp. was developed and evaluated. To construct the Multi-Pipette (MP) method, two pipettes were used to hold 1 mL of selective agar media (one pipette: Modified Oxford, MOX medium for Listeria spp. and the other pipette: Xylose Lysine Decarboxylase, XLD medium for Salmonella spp.). One mL of Brain Heart Infusion broth (BHI) was added onto the selective media through the top of 10 mL pipette. The two pipettes were placed together in a sterilized stomacher bag containing 225 mL of sterilized BHI broth that was used as enrichment broth. Listeria spp. or Salmonella spp. were enriched in the BHI broth and moved up to selective agar medium in the pipettes. They changed the color of the agar and also caused turbidity of the BHI on top of the selective agar. Three treatments were used to evaluate the MP method; (1) L. monocytogenes (103–4 CFU/mL), (2) S. typhimurium (103–4 CFU/mL), and (3) L. monocytogenes (103–4 CFU/mL) and S. typhimurium (103–4 CFU/mL) inoculated into enrichment broth with competitors [Escherichia coli (106–7 CFU/mL) and Staphylococcus aureus (106–7 CFU/mL)]. After 24 h of incubation at 35C, the colors of selective media in the MP (MOX in treatment 1, XLD in treatment 2, and MOX and XLD in treatment 3) were changed to black, and the cultures were isolated from BHI broth in the 10 mL pipettes. This approach is simple, rapid, and economical for simultaneous isolation of motile foodborne pathogens.","PeriodicalId":50067,"journal":{"name":"Journal of Rapid Methods and Automation in Microbiology","volume":"7 2","pages":"95-102"},"PeriodicalIF":0.0,"publicationDate":"2011-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4581.1999.tb00378.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63411580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

COMPARISON OF METHODS FOR PROCESSING LITTER AND AIR FILTER MATRICES FROM POULTRY HOUSES TO OPTIMIZE POLYMERASE CHAIN REACTION DETECTION OF SALMONELLA TYPHIMURIUM 鸡舍垃圾和空气过滤基质处理方法的比较优化鼠伤寒沙门菌聚合酶链反应检测

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-11-09 DOI: 10.1111/j.1745-4581.1999.tb00379.x

Y.M. KWON, C.L. WOODWARD, J. PEÑA, D.E. CORRIER, S.D. PILLAI, S.C. RICKE

{"title":"COMPARISON OF METHODS FOR PROCESSING LITTER AND AIR FILTER MATRICES FROM POULTRY HOUSES TO OPTIMIZE POLYMERASE CHAIN REACTION DETECTION OF SALMONELLA TYPHIMURIUM","authors":"Y.M. KWON, C.L. WOODWARD, J. PEÑA, D.E. CORRIER, S.D. PILLAI, S.C. RICKE","doi":"10.1111/j.1745-4581.1999.tb00379.x","DOIUrl":"10.1111/j.1745-4581.1999.tb00379.x","url":null,"abstract":"Abstract An integrated effort to reduce the occurrence of Salmonella in poultry products should include the control of Salmonella in poultry production in early stages, which requires an efficient monitoring of Salmonella spp. in poultry houses. Studies were conducted to evaluate the potential advantages of using air samples as an alternative to litter samples for PCR detection of Salmonella spp. from poultry houses. The air filter and litter samples from a poultry house were spiked with a culture of Salmonella typhimurium. The intact samples and the DNA extracted from the samples were used for amplification of Salmonella-specific DNA using hns gene specific primers. The ***hns gene was more effectively amplified with filter samples than litter samples when the intact sample was used as template. When extracted DNA was used, however, there was no difference between the two samples in their intensities of PCR products as visualized on an agarose gel. The dilution of the matrices from both sampling methods greatly increased the amplification efficiency, indicating that it was the background matrices that hindered the PCR amplification. The results in this study suggest that air filter sampling may be a labor-saving alternative to litter sampling, which can be used to increase the sensitivity of PCR detection of Salmonella spp. without prior sample processing.","PeriodicalId":50067,"journal":{"name":"Journal of Rapid Methods and Automation in Microbiology","volume":"7 2","pages":"103-111"},"PeriodicalIF":0.0,"publicationDate":"2011-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4581.1999.tb00379.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63411588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

A RAPID METHOD FOR DETECTING BACTERIA IN DRINKING WATER 一种快速检测饮用水中细菌的方法

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-11-09 DOI: 10.1111/j.1745-4581.1999.tb00382.x

JIYOUNG LEE, ROLF A. DEININGER

{"title":"A RAPID METHOD FOR DETECTING BACTERIA IN DRINKING WATER","authors":"JIYOUNG LEE, ROLF A. DEININGER","doi":"10.1111/j.1745-4581.1999.tb00382.x","DOIUrl":"10.1111/j.1745-4581.1999.tb00382.x","url":null,"abstract":"Abstract A rapid determination of the total bacterial count in drinking water is important to the operators of treatment plants and distribution systems. It will allow corrective measures in real-time, such as increasing the disinfectant dose or removing water that has high bacterial numbers. The present heterotrophic plate count (HPC) analysis takes seven days and is not useful for operational intervention. The purpose of this study was to determine if a rapid adenosine triphosphate (ATP) assay would estimate the total number of bacteria in minutes. For quality control purposes and also to test the accuracy of both the ATP and HPC test, direct enumeration of the bacteria in a water sample was done using two epifluorescence methods. One was acridine orange direct count (AODC) method, which allows enumeration of both viable and nonviable bacteria. The other was direct viable count (DVC) method, which enumerates viable bacteria. Water samples originated from local, national, and international locations. The sample selection criteria were based on proximity to the laboratory, cooperating water utilities, and the travel of the authors. The results of the study show that the rapid ATP assay is highly correlated with the conventional plate count method and the DVC method, and estimates the bacterial quality of drinking water in minutes.","PeriodicalId":50067,"journal":{"name":"Journal of Rapid Methods and Automation in Microbiology","volume":"7 2","pages":"135-145"},"PeriodicalIF":0.0,"publicationDate":"2011-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4581.1999.tb00382.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63411671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27