Journal of Rapid Methods and Automation in Microbiology最新文献_第8页

STATISTICAL VALIDATION OF THE TRACK-DILUTION PLATING METHOD FROM GROUND BEEF AND CARCASS SURFACE SAMPLES† 牛肉碎和胴体表面样品道迹稀释镀法的统计验证

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-11-09 DOI: 10.1111/j.1745-4581.1999.tb00385.x

GREGORY R. SIRAGUSA

引用次数: 19

A TWO DIMENSIONAL GEL ELECTROPHORESIS TECHNIQUE COMBINING A MICRO- AND A MINI-GEL SYSTEM FOR PROTEOMIC ANALYSIS OF FUNGAL HEAT SHOCK PROTEINS 结合微凝胶和微凝胶系统的二维凝胶电泳技术用于真菌热休克蛋白的蛋白质组学分析

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-01-19 DOI: 10.1111/j.1745-4581.2003.tb00407.x

I.J. XAVIER, G.G. KHACHATOURIANS

引用次数: 0

A MODIFIED DNA EXTRACTION MINIPREPARATION PROTOCOL FOR FUSARIUM ISOLATES 一种改良的镰刀菌分离物DNA提取微型制备方案

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-01-19 DOI: 10.1111/j.1745-4581.2003.tb00410.x

KAMEL A. ABD-ELSALAM, FRANK SCHNIEDER, JIAN-RONG GUO

引用次数: 18

Dr. Dong-Hyun Kang-New Assistant Editor 姜东炫博士-新任助理编辑

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-01-19 DOI: 10.1111/j.1745-4581.2003.tb00401.x

DANIEL Y.C. FUNG

引用次数: 0

DETECTION OF VIABLE LISTERIA MONOCYTOGENES IN MILK USING AN ELECTROCHEMICAL METHOD 电化学法检测牛奶中活的单核增生李斯特菌

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-01-19 DOI: 10.1111/j.1745-4581.2003.tb00404.x

CHUANMIN RUAN, HONG WANG, LIJU YANG, YANBIN LI

{"title":"DETECTION OF VIABLE LISTERIA MONOCYTOGENES IN MILK USING AN ELECTROCHEMICAL METHOD","authors":"CHUANMIN RUAN, HONG WANG, LIJU YANG, YANBIN LI","doi":"10.1111/j.1745-4581.2003.tb00404.x","DOIUrl":"10.1111/j.1745-4581.2003.tb00404.x","url":null,"abstract":"Abstract Growth of Listeria monocytogenes in a Listeria enrichment broth (LEB) was automatically monitored by electrochemical cyclic voltammetric scan using a gold working electrode. Changes in cyclic voltammograms were observed during growth of L. monocytogenes in LEB. The reduction peak at −0.4 V (vs Ag/AgCl) corresponding to the reduction of oxygen dissolved in LEB on cyclic voltammograms was decreasing with proliferation of L. monocytogenes, and disappeared eventually. A pair of reversible redox peaks appeared during growth of L. monocytogenes in LEB. These cyclic voltammetric characteristics can be used to detect L. monocytogenes in various samples. Threshold values (detection time) obtained from the oxygen consumption curves were inversely related to the concentrations of L. monocytogenes in the broth. A calibration curve was obtained by plotting initial cell concentrations (CFU/mL) determined by conventional plate counting, as a function of the detection time. A linear response was found on the calibration curve for L. monocytogenes between 1 ∼ 9 times 100 and 1 ∼ 9 times 105 cells/mL. The detection time was approximately 17 and 6 h for 1 ∼ 9 times 100 and 1 ∼ 9 × 105 cells/mL of viable L. monocytogenes in the broth, respectively. The method was evaluated in detection of L. monocytogenes in milk samples.","PeriodicalId":50067,"journal":{"name":"Journal of Rapid Methods and Automation in Microbiology","volume":"11 1","pages":"11-22"},"PeriodicalIF":0.0,"publicationDate":"2011-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4581.2003.tb00404.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63418605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

A COMPARATIVE STUDY OF THE ENRICHMENT MEDIA FOR RECOVERY AND DETECTION OF SALMONELLA ENTERITIDIS IN KOREAN FOOD SYSTEM 韩国食品系统中肠炎沙门氏菌回收与检测富集培养基的比较研究

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-01-19 DOI: 10.1111/j.1745-4581.2003.tb00405.x

YUN HEE CHANG, SANG MO LEE

{"title":"A COMPARATIVE STUDY OF THE ENRICHMENT MEDIA FOR RECOVERY AND DETECTION OF SALMONELLA ENTERITIDIS IN KOREAN FOOD SYSTEM","authors":"YUN HEE CHANG, SANG MO LEE","doi":"10.1111/j.1745-4581.2003.tb00405.x","DOIUrl":"10.1111/j.1745-4581.2003.tb00405.x","url":null,"abstract":"Abstract This study was performed to establish standard methods and media for detecting S. Enteritidis in Korean style foods. For the preliminary experiment, the enrichment procedures for the recovery of acid or heat injured S. Enteritidis was compared. In the mixed culture system, Salmonella Enteritidis ATCC 13076, Shigella sonnei ATCC 9290, Staphylococcus aureus ATCC 25923, Listeria monocytogenes ATCC 19111, Escherichia coli ATCC 25922 were used. S. Enteritidis in food system were evaluated by using 5 different preenrichment media (TSB: Tryptic Soy Broth, LB: Lactose Broth, BPW: Buffered Peptone Water, SB: Selenite Broth, SCB: Selenite Cystine Broth) and 2 different enrichment media (TT: Tetrathionate Broth, RV: Rappaport and Vassiliadis).TSB was the most effective medium to detect acid and heat injured S. Enteritidis after 6 h incubation, but SB and SCB were also effective after 24 h incubation. For the recovery of heat injured S. Enteritidis at 65C, 5 pre-enrichment media did not show significant differences (P<0.05). But after 6 h incubation, TSB and BPW were more effective than other media for the recovery of heat injured S. Enteritidis at 60C. BPW, TSB and TT were most effective for detecting S. Enteritidis in Kimbob (rice roll with lover), salad, Japchae (Chinese noodle with vegetable), mussel, pan-fried fish fillet, broiler carcasses, ground pork and Jokbal (boiled pork hock). TSB and TT were most effective for milk and eggs, and SCB was also effective for broiler carcasses.","PeriodicalId":50067,"journal":{"name":"Journal of Rapid Methods and Automation in Microbiology","volume":"11 1","pages":"23-34"},"PeriodicalIF":0.0,"publicationDate":"2011-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4581.2003.tb00405.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63418661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FACTORS AFFECTING THE BACTERIAL CAPTURE EFFICIENCY OF IMMUNO BEADS: A COMPARISON BETWEEN BEADS WITH DIFFERENT SIZE AND DENSITY† 影响免疫微球细菌捕获效率的因素:不同大小和密度微球的比较

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-01-19 DOI: 10.1111/j.1745-4581.2003.tb00406.x

S.-I. TU, J. UKNALIS, M. GORE, P. IRWIN, I. FEDER

{"title":"FACTORS AFFECTING THE BACTERIAL CAPTURE EFFICIENCY OF IMMUNO BEADS: A COMPARISON BETWEEN BEADS WITH DIFFERENT SIZE AND DENSITY†","authors":"S.-I. TU, J. UKNALIS, M. GORE, P. IRWIN, I. FEDER","doi":"10.1111/j.1745-4581.2003.tb00406.x","DOIUrl":"10.1111/j.1745-4581.2003.tb00406.x","url":null,"abstract":"Abstract Biotinylated anti E. coli O157 antibodies were linked to streptavidin coated magnetic and polystyrene beads of different sizes and densities. These beads were then used to capture cultured E. coli O157:H7. Complexes formed between fluorescein-conjugated anti E. coli O157 antibodies and urease-conjugated anti-fluorescein antibodies further labeled bead-captured bacteria. The extent of the bacterial capture was related to the signal intensity of a light addressable potentiometric sensor (LAPS) that measured the pH changes associated with urease-catalyzed hydrolysis of urea. Under the conditions of applying the same amounts of biotinylated anti E. coli O157 antibody to beads, analyses of the capture kinetics indicated that heavier beads exhibited a better efficiency in capturing the target bacteria than that associated with the lighter beads of the same size. Furthermore, with the same density, larger bead diameters were more effective in capturing the bacteria. Since sedimentation volumes for the heavier and larger beads are greater than those of the lighter and smaller beads, more interactions with the target by the heavier and larger beads are expected. Consequently, the observation is in agreement with a hydrodynamic kinetic model that predicts the bacterial capture is controlled by the sedimentation motion of the beads.","PeriodicalId":50067,"journal":{"name":"Journal of Rapid Methods and Automation in Microbiology","volume":"11 1","pages":"35-46"},"PeriodicalIF":0.0,"publicationDate":"2011-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4581.2003.tb00406.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63418725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

DEVELOPMENT OF A MICROTITER - BASED ASSAY FOR ASSESSMENT OF VIRULENCE RESPONSE IN SALMONELLA TYPHIMURIUM EXPOSED TO FRESH PRODUCE EXTRACTS 建立以微量滴度为基础的鼠伤寒沙门菌暴露于新鲜农产品提取物毒力反应评估方法

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-01-19 DOI: 10.1111/j.1745-4581.2003.tb00403.x

J.D. NUTT, X. LI, I.B. ZABALA-DÍAZ, C.L. WOODWARD, S.C. RICKE

{"title":"DEVELOPMENT OF A MICROTITER - BASED ASSAY FOR ASSESSMENT OF VIRULENCE RESPONSE IN SALMONELLA TYPHIMURIUM EXPOSED TO FRESH PRODUCE EXTRACTS","authors":"J.D. NUTT, X. LI, I.B. ZABALA-DÍAZ, C.L. WOODWARD, S.C. RICKE","doi":"10.1111/j.1745-4581.2003.tb00403.x","DOIUrl":"10.1111/j.1745-4581.2003.tb00403.x","url":null,"abstract":"Abstract The purpose of this research was to develop a microtiter-based assay procedure for assessment of virulence gene response of Salmonella Typhimurium to fresh homogenized vegetables. The microtiter procedure was adapted from a assay for β-galactosidase activity of a hilA fusion strain of S. Typhimurium with volumes of all reagents adjusted for microtiter plate wells. S. Typhimurium exposed to pH 7 saline yielded a higher level of hilA expression than responses after incubation in pH 4, 5 and 6 saline. Bacterial cells exposed to broccoli extracts yielded the higher levels of hilA expression while bell pepper and tomato yielded the overall lowest amounts of hilA expression. From this study, it appears that fresh produce extracts have different abilities to significantly alter virulence response in Salmonella. In conclusion, the hilA fusion stain assay can be successfully adapted to microtiter plates for testing Salmonella virulence response to vegetable extracts.","PeriodicalId":50067,"journal":{"name":"Journal of Rapid Methods and Automation in Microbiology","volume":"11 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2011-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4581.2003.tb00403.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63418538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

A RAPID AND EFFECTIVE METHOD FOR TOTAL RNA EXTRACTION FROM CONIDIA AND MYCELIUM OF SEPTORIA TRITICI 一种快速有效地提取小麦隔孢孢子和菌丝体总RNA的方法

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-01-19 DOI: 10.1111/j.1745-4581.2003.tb00408.x

JIAN-RONG GUO, KAMEL A. ABD-ELSALAM, FRANK SCHNIEDER, JOSEPH-ALEXANDER VERREET

{"title":"A RAPID AND EFFECTIVE METHOD FOR TOTAL RNA EXTRACTION FROM CONIDIA AND MYCELIUM OF SEPTORIA TRITICI","authors":"JIAN-RONG GUO, KAMEL A. ABD-ELSALAM, FRANK SCHNIEDER, JOSEPH-ALEXANDER VERREET","doi":"10.1111/j.1745-4581.2003.tb00408.x","DOIUrl":"10.1111/j.1745-4581.2003.tb00408.x","url":null,"abstract":"Abstract An effective and rapid method for RNA extraction from conidia and mycelium of S. tritici was developed, in which guanidinium isothiocyanate, CTAB and insoluble PVP were combined in one step, simplifying the extraction process greatly. The procedure can be completed in less than 6 h. Yield and purity of RNA could be estimated by electrophoresis on 1% formaldhyde-denatured agarose gel and by measuring the absorbance at 280, 260, 230 and 210 nm by UV spectroscopy. The result of electrophoresis showed two clear bands of 18S and 28S rRNA and no DNA contamination in the isolated RNA solution. The ratios A260/A280, A260/A230, A260/A210 were 1.76–1.82, 2.79–3.21 and 0.81–0.86, respectively. A high yield with 532–881 ng total RNA per gram fresh conidia and mycelium of S. tritici was obtained. The RNA was of sufficient purity for RT-PCR to detect the expression of β-tubulin gene in S. tritici. This protocol may also be suitable for RNA extraction from spores and mycelium of other fungi.","PeriodicalId":50067,"journal":{"name":"Journal of Rapid Methods and Automation in Microbiology","volume":"11 1","pages":"53-59"},"PeriodicalIF":0.0,"publicationDate":"2011-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4581.2003.tb00408.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63419084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

DEVELOPMENT OF A MULTIPLEX PCR ASSAY FOR THE SPECIFIC DETECTION OF SALMONELLA, CAMPYLOBACTER JEJUNI, ESCHERICHIA COLI O157:H7, AND LISTERIA MONOCYTOGENES 建立沙门氏菌、空肠弯曲杆菌、大肠杆菌o157: h7和单核细胞增生李斯特菌特异性检测的多重PCR方法

Journal of Rapid Methods and Automation in Microbiology Pub Date : 2011-01-19 DOI: 10.1111/j.1745-4581.2003.tb00409.x

C. GILBERT, A. O'LEARY, D. WINTERS, M. SLAVIK

引用次数: 8