牛肉碎和胴体表面样品道迹稀释镀法的统计验证

GREGORY R. SIRAGUSA
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引用次数: 19

摘要

摘要:本研究采用track -稀释法对接种沙门氏菌或大肠杆菌O157:H7的碎牛肉和牛胴体表面组织样品进行了快速、简便的活菌计数测定,并与螺旋平板法进行了比较。基于数据集(n = 125个样本)的统计分析,径迹稀释法结果无差异(P >0.1),比螺旋镀法得到的高。螺旋镀法和径迹稀释法的散点图线性相关表明两种方法的一致性较高(r2 = 0.97)。迹迹稀释法是估计细菌计数大于100 cfu/mL的肉类动物来源样品的活菌计数的有效方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
STATISTICAL VALIDATION OF THE TRACK-DILUTION PLATING METHOD FROM GROUND BEEF AND CARCASS SURFACE SAMPLES†

Abstract A rapid and easy method of obtaining a viable culture count, the Track-Dilution method, was evaluated and compared to Spiral Plating to obtain viable culture counts from ground beef samples and beef carcass surface tissue samples inoculated with salmonellae or Escherichia coli O157:H7. Based on the statistical analysis of the dataset (n = 125 samples), the Track-Dilution method results were not different (P > 0.1) than those obtained from Spiral Plating. Linear correlation of the scatterplot of Spiral Plating and Track-Dilution indicated a high level of agreement between these two methods (r2 = 0.97). The Track-Dilution method was a valid method for estimating viable culture counts for meat animal-derived samples with bacterial counts of more than 100 cfu/mL.

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Journal of Rapid Methods and Automation in Microbiology
Journal of Rapid Methods and Automation in Microbiology 生物-生物工程与应用微生物
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