{"title":"A TWO DIMENSIONAL GEL ELECTROPHORESIS TECHNIQUE COMBINING A MICRO- AND A MINI-GEL SYSTEM FOR PROTEOMIC ANALYSIS OF FUNGAL HEAT SHOCK PROTEINS","authors":"I.J. XAVIER, G.G. KHACHATOURIANS","doi":"10.1111/j.1745-4581.2003.tb00407.x","DOIUrl":null,"url":null,"abstract":"<p><b>Abstract </b> <i>A two-dimensional gel electrophoresis (2-DGE) technique is described here that combines isoelectric focusing (IEF) based separation of proteins using the PhastGel IEF in the first dimension and the Criterion precast mini-gels in the second dimension. The technique is rapid (2.5 to 3 h including silver staining), convenient, sensitive with high resolution and enables simultaneous analysis of up to 24 samples. This technique also eliminates any inconsistencies arising from gel to gel variation, either from electrophoresis running conditions or differential staining times, especially as in the case of silver stains. The rapidity and efficacy of this technique is demonstrated by the proteome-based analysis of the induction, enhancement and repression of proteins in heat shocked</i> Beauveria bassiana, <i>ah entomopathogenic fungus.</i></p>","PeriodicalId":50067,"journal":{"name":"Journal of Rapid Methods and Automation in Microbiology","volume":"11 1","pages":"47-52"},"PeriodicalIF":0.0000,"publicationDate":"2011-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4581.2003.tb00407.x","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Rapid Methods and Automation in Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/j.1745-4581.2003.tb00407.x","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Abstract A two-dimensional gel electrophoresis (2-DGE) technique is described here that combines isoelectric focusing (IEF) based separation of proteins using the PhastGel IEF in the first dimension and the Criterion precast mini-gels in the second dimension. The technique is rapid (2.5 to 3 h including silver staining), convenient, sensitive with high resolution and enables simultaneous analysis of up to 24 samples. This technique also eliminates any inconsistencies arising from gel to gel variation, either from electrophoresis running conditions or differential staining times, especially as in the case of silver stains. The rapidity and efficacy of this technique is demonstrated by the proteome-based analysis of the induction, enhancement and repression of proteins in heat shocked Beauveria bassiana, ah entomopathogenic fungus.
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